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CN110903979A - Temporary storage device for biological cell culture dish - Google Patents

Temporary storage device for biological cell culture dish Download PDF

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Publication number
CN110903979A
CN110903979A CN201911138850.7A CN201911138850A CN110903979A CN 110903979 A CN110903979 A CN 110903979A CN 201911138850 A CN201911138850 A CN 201911138850A CN 110903979 A CN110903979 A CN 110903979A
Authority
CN
China
Prior art keywords
cabinet body
connecting pipe
cell culture
storage device
temporary storage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201911138850.7A
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Chinese (zh)
Inventor
宫莉
王超普
张辉华
陈柏东
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Foshan University
Original Assignee
Foshan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Foshan University filed Critical Foshan University
Priority to CN201911138850.7A priority Critical patent/CN110903979A/en
Publication of CN110903979A publication Critical patent/CN110903979A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a temporary storage device for biological cell culture dishes, which comprises a cabinet body, wherein a plurality of horizontal partition plates are fixedly arranged in the cabinet body, a plurality of placing grooves are arranged on the partition plates, a cylinder is fixedly arranged at the top of the cabinet body, a piston is connected in the cylinder in a sliding manner, a connecting rod is hinged to the right side of the piston, a first one-way valve is connected to the upper side of the cylinder, the other end of the first one-way valve is connected to a filter through a pipeline, the filter comprises a rectangular shell, a first penetrating connecting pipe is fixedly connected to one end of the shell, an air filter element is connected to the inner end of the first connecting pipe, a second penetrating connecting pipe is connected to the shell, and the first connecting pipe is connected to the inner cavity of the cabinet. This device is internal with the air escape cabinet after the filtration purification, and the air flow in the reinforcing cabinet guarantees that oxygen is sufficient, provides good growing environment for the cell in the culture dish.

Description

Temporary storage device for biological cell culture dish
Technical Field
The invention relates to the technical field of biology, in particular to a temporary storage device for a biological cell culture dish.
Background
In biological teaching, especially in biological experiments, biological cells are cultured and observed, which is a biological experiment that needs to be done frequently, and the biological cells to be cultured are arranged in a culture dish and then are put into an incubator with well-set temperature and humidity conditions for culture in the conventional biological cell culture device. In actual use, a plurality of culture dishes can be manufactured at one time, and the manufactured culture dishes are placed into a special incubator in the future and need to be kept in a temporary storage device, or are exposed to the outside and easily polluted. The conventional temporary storage device is mostly replaced by a common box or cabinet, and the closed box or cabinet body is not beneficial to aerobic cell culture.
Disclosure of Invention
The invention aims to provide a temporary storage device for biological cell culture dishes, which solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides an interim strorage device of biological cell culture dish, includes the cabinet body, the internal fixed mounting of cabinet has the horizontally baffle of a plurality of, be equipped with a plurality of standing groove on the baffle, the top fixed mounting of the cabinet body has the drum, sliding connection has the piston in the drum, the right side of piston articulates there is the connecting rod, the upside of drum is connected with first check valve, the pipe connection for the other end of first check valve is to the filter, the filter includes the casing of rectangle, the first connecting pipe that the one end fixedly connected with of casing runs through, the inner end of first connecting pipe is connected with air filter, the second that is connected with of casing runs through is taken over, the inner chamber of the pipe connection to the cabinet body for the first connecting pipe.
Preferably, a cabinet door is installed on the front side of the cabinet body through a hinge, and an ultraviolet lamp is installed on the inner side of the cabinet door.
Preferably, the top of the cabinet body is provided with an L-shaped support, the side surface of the support is fixedly connected with a U-shaped mounting rack, the mounting rack is rotatably connected with a crankshaft, and a journal of the crankshaft is rotatably connected with the other end of the connecting rod.
Preferably, the motor is installed to the upside of mounting bracket, the output and the bent axle of motor are connected.
Preferably, a second one-way valve is arranged at the left end of the cylinder and penetrates through the cylinder, and the first one-way valve is communicated with the second connecting pipe.
Specifically, the bottom of the cabinet body is provided with a circular air outlet, and the diameter of the air outlet is 2-3 cm.
Compared with the prior art, the invention has the beneficial effects that: after the motor is started, the crankshaft rotates, the piston is pulled to reciprocate through the connecting rod, air is continuously extracted from the outside and conveyed to the filter, dust and impurities in the air are removed by an air filter element of the filter, the filtered and purified air is discharged into the cabinet body, air flow in the cabinet is enhanced, sufficient oxygen is guaranteed, and a good growing environment is provided for cells in the culture dish.
Drawings
FIG. 1 is a schematic side view of the present invention;
FIG. 2 is a schematic front view of the present invention;
FIG. 3 is an enlarged view of a portion of FIG. 2;
FIG. 4 is a schematic view of the filter structure of the present invention.
In the figure: 1. a cabinet body; 2. a cabinet door; 3. a partition plate; 4. a placement groove; 5. an ultraviolet lamp; 6. a filter; 61. a housing; 62. an air filter element; 63. a first connecting pipe; 64. a second adapter tube; 7. a cylinder; 8. A piston; 9. a connecting rod; 10. a crankshaft; 11. a mounting frame; 12. a motor; 13. a second one-way valve; 14. A first one-way valve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: the utility model provides a interim strorage device of biological cell culture dish, includes the cabinet body 1, the internal fixed mounting of cabinet has a plurality of horizontally baffle 3, be equipped with a plurality of standing groove 4 on the baffle 3, the top fixed mounting of the cabinet body 1 has drum 7, sliding connection has piston 8 in the drum 7, piston 8's right side articulates there is connecting rod 9, the upside of drum 7 is connected with first check valve 14, the other end of first check valve 14 is with pipe connection to filter 6, filter 6 includes the casing 61 of rectangle, the first connecting pipe 63 that the one end fixedly connected with of casing 61 runs through, the inner end of first connecting pipe 63 is connected with air filter 62, the second of being connected with of casing 61 running through takes over 64, the inner chamber of the cabinet body 1 of pipe connection for 63.
Specifically, a cabinet door 2 is installed on the front side of the cabinet body 1 through a hinge, and an ultraviolet lamp 5 is installed on the inner side of the cabinet door 2. Before the culture dish is placed, the inside is sterilized and disinfected by an ultraviolet lamp 5.
Specifically, the support of L shape is installed at the top of the cabinet body 1, the side fixedly connected with U-shaped mounting bracket 11 of support, mounting bracket 11 rotates and is connected with bent axle 10, the axle journal of bent axle 10 rotates with the other end of connecting rod 9 and is connected. The crankshaft 10 pulls the piston 8 to reciprocate through the connecting rod 9 when rotating.
Specifically, motor 12 is installed to the upside of mounting bracket 11, the output of motor 12 is connected with bent axle 10.
Specifically, a second one-way valve 13 is installed at the left end of the cylinder 7, and the first one-way valve 14 is communicated with the second connecting pipe 64. The second check valve 13 allows gas to be introduced into the cylinder 7, and the gas in the cylinder 7 is discharged by the first check valve 14.
Specifically, the bottom of the cabinet body 1 is provided with a circular air outlet, and the diameter of the air outlet is 2-3 cm. The air outlet is used for discharging air in the cabinet, so that the internal air pressure and the external air pressure are balanced.
The working principle is as follows: when in use, the culture dish is clamped in the placing groove 4, and then the cabinet door 2 is closed. After the motor 12 is started, the crankshaft 10 rotates to drive the piston 8 to reciprocate through the connecting rod 9, when the piston 8 moves rightwards, air is sucked into the cylinder 7 through the second one-way valve 13, when the piston 8 moves leftwards, air in the cylinder 7 is pressed out through the first one-way valve 14, the pressed air is filtered through the filter 6, dust and impurities in the air are removed through the air filter element 62 of the filter 6, the filtered and purified air is discharged into the cabinet body 1, air flow in the cabinet is enhanced, and oxygen is sufficient.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. The utility model provides a biological cell culture dish temporary storage device, includes cabinet body (1), its characterized in that: a plurality of horizontal partition plates (3) are fixedly arranged in the cabinet body (1), a plurality of placing grooves (4) are arranged on the partition plates (3), a cylinder (7) is fixedly arranged at the top of the cabinet body (1), a piston (8) is connected in the cylinder (7) in a sliding way, the right side of the piston (8) is hinged with a connecting rod (9), the upper side of the cylinder (7) is connected with a first one-way valve (14), the other end of the first one-way valve (14) is connected to the filter (6) by a pipeline, the filter (6) comprises a rectangular shell (61), one end of the shell (61) is fixedly connected with a first connecting pipe (63) which penetrates through the shell, the inner end of the first connecting pipe (63) is connected with an air filter element (62), the shell (61) is connected with a second connecting pipe (64) which penetrates through the shell, the first connecting pipe (63) is connected to the inner cavity of the cabinet body (1) through a pipeline.
2. The temporary storage device for biological cell culture dishes, according to claim 1, wherein: the front side of the cabinet body (1) is provided with a cabinet door (2) through a hinge, and the inner side of the cabinet door (2) is provided with an ultraviolet lamp (5).
3. The temporary storage device for biological cell culture dishes, according to claim 1, wherein: the support of L shape is installed at the top of the cabinet body (1), the side fixedly connected with U-shaped's of support mounting bracket (11), mounting bracket (11) rotate and are connected with bent axle (10), the axle journal of bent axle (10) rotates with the other end of connecting rod (9) and is connected.
4. The temporary storage device for biological cell culture dishes, according to claim 3, wherein: the motor (12) is installed to the upside of mounting bracket (11), the output and the bent axle (10) of motor (12) are connected.
5. The temporary storage device for biological cell culture dishes, according to claim 1, wherein: and a second one-way valve (13) penetrating through the cylinder (7) is installed at the left end of the cylinder, and the first one-way valve (14) is communicated with the second connecting pipe (64).
6. The temporary storage device for biological cell culture dishes, according to claim 1, wherein: the cabinet body (1) bottom is equipped with circular shape venthole, the diameter of venthole is 2 ~ 3 cm.
CN201911138850.7A 2019-11-20 2019-11-20 Temporary storage device for biological cell culture dish Pending CN110903979A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911138850.7A CN110903979A (en) 2019-11-20 2019-11-20 Temporary storage device for biological cell culture dish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911138850.7A CN110903979A (en) 2019-11-20 2019-11-20 Temporary storage device for biological cell culture dish

Publications (1)

Publication Number Publication Date
CN110903979A true CN110903979A (en) 2020-03-24

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113528340A (en) * 2021-08-04 2021-10-22 河南省肿瘤医院 A tumor cell culture system

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998051777A1 (en) * 1997-05-15 1998-11-19 Genx International, Inc. Improved filtering system and method for incubators
CN208882392U (en) * 2018-08-01 2019-05-21 郑州澍青医学高等专科学校 A kind of Embryo tissue storing unit
CN110179265A (en) * 2019-04-25 2019-08-30 杜衍生 A kind of medical instrument dedusting placement cabinet
CN209397225U (en) * 2018-11-13 2019-09-17 东华理工大学 A kind of biotechnology cell culture apparatus
CN209548434U (en) * 2018-10-30 2019-10-29 启迪汉洱康(嘉兴)生物科技有限公司 A kind of multiple Sterilization disinfectant cabinet of cell culture
CN209584172U (en) * 2018-11-15 2019-11-05 马浩予 A kind of culture apparatus of biological inoculum

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998051777A1 (en) * 1997-05-15 1998-11-19 Genx International, Inc. Improved filtering system and method for incubators
CN208882392U (en) * 2018-08-01 2019-05-21 郑州澍青医学高等专科学校 A kind of Embryo tissue storing unit
CN209548434U (en) * 2018-10-30 2019-10-29 启迪汉洱康(嘉兴)生物科技有限公司 A kind of multiple Sterilization disinfectant cabinet of cell culture
CN209397225U (en) * 2018-11-13 2019-09-17 东华理工大学 A kind of biotechnology cell culture apparatus
CN209584172U (en) * 2018-11-15 2019-11-05 马浩予 A kind of culture apparatus of biological inoculum
CN110179265A (en) * 2019-04-25 2019-08-30 杜衍生 A kind of medical instrument dedusting placement cabinet

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
徐庚达等: "生物洁净室在人脑瘤细胞体外长期培养的应用", 《苏州大学学报(医学版)》 *
王亮 等: "《流体力学与流体机械》", 31 January 2016, 吉林大学出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113528340A (en) * 2021-08-04 2021-10-22 河南省肿瘤医院 A tumor cell culture system

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Application publication date: 20200324

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