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CN110603327A - Pcr引物对及其应用 - Google Patents

Pcr引物对及其应用 Download PDF

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Publication number
CN110603327A
CN110603327A CN201780090488.3A CN201780090488A CN110603327A CN 110603327 A CN110603327 A CN 110603327A CN 201780090488 A CN201780090488 A CN 201780090488A CN 110603327 A CN110603327 A CN 110603327A
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China
Prior art keywords
primer
sequence
pcr
universal
pair
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CN201780090488.3A
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杨林
蒋浩君
曾鹏
庄雪寒
高雅
张艳艳
蒋慧
郭晶
陈芳
徐讯
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MGI Tech Co Ltd
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MGI Tech Co Ltd
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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Abstract

提供了PCR引物对及其应用。其中，该PCR引物对包括：第一引物和第二引物，其中，所述第一引物包含第一特异性序列、第一随机序列和第一通用序列，所述第一特异性序列位于所述第一引物的3’端，所述第一随机序列位于所述第一引物的5’端，所述第一通用序列位于所述第一特异性序列和所述第一随机序列之间，所述第二引物包含第二特异性序列、第二随机序列和第二通用序列，所述第二特异性序列位于所述第二引物的3’端，所述第二随机序列位于所述第二引物的5’端，所述第二通用序列位于所述第二特异性序列和所述第二随机序列之间，其中，所述第一随机序列和所述第二随机序列反向互补。

Description

PCT国内申请，说明书已公开。

Claims (22)

  1. PCT国内申请，权利要求书已公开。
CN201780090488.3A 2017-06-20 2017-06-20 Pcr引物对及其应用 Pending CN110603327A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2017/089199 WO2018232598A1 (zh) 2017-06-20 2017-06-20 Pcr引物对及其应用

Publications (1)

Publication Number Publication Date
CN110603327A true CN110603327A (zh) 2019-12-20

Family

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CN201780090488.3A Pending CN110603327A (zh) 2017-06-20 2017-06-20 Pcr引物对及其应用

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US (1) US12104205B2 (zh)
EP (1) EP3643789A4 (zh)
CN (1) CN110603327A (zh)
WO (1) WO2018232598A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111455114A (zh) * 2020-05-22 2020-07-28 深圳华大智造科技有限公司 SARS-CoV-2的高通量检测试剂盒

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102175718B1 (ko) * 2016-03-25 2020-11-06 카리우스, 인코포레이티드 합성 핵산 스파이크-인
US11760995B2 (en) * 2017-06-20 2023-09-19 Mgi Tech Co., Ltd. PCR primer pair and application thereof
EP3884065B1 (en) 2018-11-21 2024-08-14 Karius, Inc. Direct-to-library methods, systems, and compositions
CN110029147B (zh) * 2019-01-25 2023-03-24 上海何因生物科技有限公司 一种单管实现连续区域扩增同时降低非特异性扩增的多重pcr方法
CN117343929B (zh) * 2023-12-06 2024-04-05 广州迈景基因医学科技有限公司 一种pcr随机引物及用其加强靶向富集的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090130720A1 (en) * 2001-01-19 2009-05-21 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
CN101809170A (zh) * 2007-09-28 2010-08-18 3M创新有限公司 用于检测核酸的双寡核苷酸方法
US20160026758A1 (en) * 2012-02-27 2016-01-28 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
WO2016181128A1 (en) * 2015-05-11 2016-11-17 Genefirst Ltd Methods, compositions, and kits for preparing sequencing library

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003276765A1 (en) * 2003-11-10 2005-05-26 Seegene, Inc. Method for amplifying unknown dna sequence adjacent to known sequence
CA2615323A1 (en) * 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
US9410173B2 (en) * 2012-10-24 2016-08-09 Clontech Laboratories, Inc. Template switch-based methods for producing a product nucleic acid
CN109971826B (zh) * 2014-01-31 2022-12-30 斯威夫特生物科学股份有限公司 用于加工dna底物的改进方法
CN103820561B (zh) * 2014-03-10 2016-04-20 广西壮族自治区农业科学院园艺研究所 一种柑橘黄龙病亚洲种巢氏pcr扩增检测体系及应用

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090130720A1 (en) * 2001-01-19 2009-05-21 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
CN101809170A (zh) * 2007-09-28 2010-08-18 3M创新有限公司 用于检测核酸的双寡核苷酸方法
US20160026758A1 (en) * 2012-02-27 2016-01-28 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
WO2016181128A1 (en) * 2015-05-11 2016-11-17 Genefirst Ltd Methods, compositions, and kits for preparing sequencing library

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JAVIER GARCÍA-NAFRÍA ET AL.: "IVA cloning: A single-tube universal cloning system exploiting bacterial In Vivo Assembly", 《SCIENTIFIC REPORTS》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111455114A (zh) * 2020-05-22 2020-07-28 深圳华大智造科技有限公司 SARS-CoV-2的高通量检测试剂盒
CN111455114B (zh) * 2020-05-22 2023-09-05 深圳华大智造科技股份有限公司 SARS-CoV-2的高通量检测试剂盒

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EP3643789A4 (en) 2021-01-06
US12104205B2 (en) 2024-10-01
EP3643789A1 (en) 2020-04-29
WO2018232598A1 (zh) 2018-12-27
US20200216874A1 (en) 2020-07-09

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