CN110389057B

CN110389057B - Integrated multifunctional biological sample processor

Info

Publication number: CN110389057B
Application number: CN201810364982.0A
Authority: CN
Inventors: 李响球; 罗垲炜; 李纪盛; 李润海; 倪军
Original assignee: Shanghai Borton Medical Equipment Co ltd
Current assignee: Shanghai Borton Medical Equipment Co ltd
Priority date: 2018-04-23
Filing date: 2018-04-23
Publication date: 2024-12-31
Anticipated expiration: 2038-04-23
Also published as: CN110389057A

Abstract

The invention relates to an integrated multifunctional biological sample processor, which comprises a test tube body, wherein the test tube body comprises a sampling treatment section and an optical observation section, the sampling treatment section is communicated with the optical observation section to form the whole test tube body, a test tube plug is arranged on an upper cover of a test tube body inlet at one end of the sampling treatment section, the optical observation section comprises an observation part, a biological sample entering the sampling treatment section is processed to form sampling liquid, the sampling liquid enters the optical observation section, and external inspection equipment observes a sample and acquires sample parameters through the observation part. The invention adopts the integrated processor to realize the integration of various biological sample processing functions, and has the characteristics of convenient operation and high efficiency.

Description

Integrated multifunctional biological sample processor

Technical Field

The invention relates to an integrated multifunctional biological sample processor, in particular to an intelligent integrated multifunctional biological sample processor, and belongs to the field of biological sample processing.

Background

Current biological sample processing procedures in the field of medical testing or life sciences generally include manual procedures and instrument procedures. The manual operation process is that an operator collects a sample by using a sampling needle, a sampling sample, a sampling spoon and the like and puts the sample into a test tube, if the sample is to be diluted, the sample is mixed uniformly after the diluent is added, and if the sample is to be centrifuged (urine sediment, blood and the like), the sample is to be centrifuged according to the requirement. Inserting a liquid-transferring gun into a suction nozzle, sucking supernatant by the liquid-transferring gun or sucking sediment after pouring supernatant, injecting a sample in the suction head of the liquid-transferring gun into a glass slide or a counting pool plate, adding a cover glass on the glass slide or the counting pool, and then putting the glass slide or the counting pool under a microscope to watch the morphology and the count of the components. Another way is to inject the sample in the pipette tip into a dipstick reaction plate or other reactor, then place the reaction plate or reactor into an analytical instrument for detection and analysis, and finally pipette tip destroy. In the automatic analyzer treatment process, the pipette is changed into an automatic analyzer suction needle and a silica gel pipeline, the suction needle sucks samples and then injects the samples into a glass slide or a reactor, or the samples are sucked into a counting pool through the silica gel pipeline after the suction needle sucks the samples, and the other samples are the same as manual operation. The treatment process of each sample comprises the steps of liquid suction, liquid discharge, cleaning and disinfection.

The pipetting process of the pipette is particularly complicated, namely, the pipette needs to add a suction head, then move a sample into a counting pool from a test tube, and then throw the suction head away, so that the time and the labor are consumed. And the problems of influencing quantitative accuracy quality, biological sample pollution and the like in the process of pouring out the supernatant and transferring the sample are also related. The automatic instrument can only replace hands and a pipette with a sample sucking needle and a manipulator, and meanwhile, the automatic instrument also involves pipeline blockage, a large amount of waste liquid and reagent are consumed by cleaning and disinfecting pipelines, particularly when the centrifugal sediment is sucked, the sample sucking needle is not sucked well because of high sediment viscosity, and the sample sucking needle is not conveyed to a flow counting cell through a silica gel pipe, so that the automatic instrument is time-consuming and labor-consuming. And some samples are required to be subjected to layered liquid extraction after centrifugation, for example, the supernatant liquid of centrifugation is required to be extracted from some detection items of blood samples, and the cell sediment after centrifugation, for example, the cell sediment after centrifugation is required to be extracted from urine sediment, and the upper layer and the lower layer are mutually influenced in the liquid extraction process, so that the operation is not facilitated.

Disclosure of Invention

The invention discloses a novel scheme of an integrated multifunctional biological sample processor, which integrates various biological sample processing functions by adopting the integrated processor, and solves the problems of troublesome operation and low efficiency of the existing scheme.

The invention relates to an integrated multifunctional biological sample processor, which comprises a test tube body, wherein the test tube body comprises a sampling treatment section and an optical observation section, the sampling treatment section is communicated with the optical observation section to form the whole test tube body, a test tube plug is arranged on an upper cover of a test tube body inlet at one end of the sampling treatment section, the optical observation section comprises an observation part, a biological sample entering the sampling treatment section is processed to form a sampling liquid, and the sampling liquid enters the optical observation section, and external inspection equipment observes a sample and acquires sample parameters through the observation part.

Further, the partial side wall of the sampling processing section of the scheme is of a cylindrical surface structure, the partial side wall of the observation part of the optical observation section is of a plane structure, the cylindrical surface structure and the plane structure are connected through an inclined plane structure to form the whole partial side wall of the test tube body, and the external inspection equipment observes samples and collects sample parameters through the plane structure.

Further, the test tube stopper of this scheme be the appearance with test tube body import inboard matched columnar structure is equipped with a plurality of bellied sealing rings that extend along circumference on above-mentioned columnar structure's the outside, promotes the test tube stopper and covers tightly and arrange the sampling liquid into test paper reaction unit, and the sealing ring prevents the biological sample in the test tube body extravasation.

Further, be equipped with the hollow ball of elasticity on the outer end of test tube stopper of this scheme, extrude the hollow ball of elasticity and discharge the test paper reaction unit with the sampling liquid.

Further, the test tube stopper of this scheme includes the test tube stopper cavity, and the test tube stopper cavity is fixed in the test tube body import, and the test tube stopper cavity is equipped with the flowing back piston on the position of test tube body import side, promotes flowing back piston and discharges into test paper reaction unit with the sampling liquid.

Further, be equipped with the columnar ration flowing back piston on the outer end of the test tube stopper of this scheme, the outside of columnar ration flowing back piston is equipped with the flowing back scale along flowing back direction, promotes columnar ration flowing back piston and will sample liquid and arrange into test paper response subassembly according to the flowing back scale.

Further, the test tube stopper of this scheme is established and is equipped with the square spoon of sample on the internal one end of test tube, and the square spoon of sample includes spoon head, spoon handle, and the spoon handle is tubular structure, and the square spoon of sample is established on the connecting handle of test tube stopper on one end in the test tube through spoon handle cover.

Still further, the spoon head of the sampling Fang Shao of the scheme is provided with a plurality of through hole structures.

Further, the test tube stopper of this scheme is established and is equipped with the fiber test on the internal one end of test tube, and the fiber test includes fiber test head, fiber test rod, and fiber test rod is tubular structure, and the fiber test is established on the connecting handle of test tube stopper on the internal one end of test tube through fiber test rod cover.

Further, be equipped with sampling baffle A in the back end of the sampling section of this scheme test tube body, be equipped with the circulation clearance between sampling baffle A and the inside wall of sampling section of handling, sampling baffle A includes slope guide face, and biological sample in the sampling section of handling is through slope guide face deposit to the observation portion of optical observation section after centrifugal processing.

Further, be equipped with sampling baffle B in the back end of the sampling section of this scheme's test tube body, sampling baffle B and the inside wall of sampling section of handling are along circumference close combination, are equipped with a plurality of filtration through-holes on the sampling baffle B, and biological sample in the sampling section of handling gets into the observation portion of optical observation section after filtering through the filtration through-hole.

Further, be equipped with sampling combination baffle in the back end of the section is handled in the sampling of test tube body of this scheme, sampling combination baffle and the inside wall of sampling processing section paste the tight contact along circumference, sampling combination baffle includes and connects gradually through the elastic part and forms holistic baffle a of sampling combination baffle, baffle b, baffle c, be equipped with the connecting rod of being connected with the test tube stopper on the baffle b, the inner movable sleeve of connecting rod is established in fixed guide ring, baffle a's distal end side passes through the elastic part and is connected with the guide ring, baffle c's distal end side passes through the elastic part and is connected with the guide ring, promote test tube stopper linkage connecting rod promotion baffle b and make sampling combination baffle warp and roll up and form the circulation clearance with between the sampling processing section inside wall, biological sample in the sampling processing section gets into the observation portion of optical observation section through the circulation clearance, withdraw from the test tube stopper, sampling combination baffle resets under the effect of elastic part.

Further, be equipped with the sampling baffle in the sampling processing section of this scheme's the test tube body, the observation portion that the one end of sampling baffle extended into the optics observation section forms and carries the sample piece, and outside check out equipment passes through the sample on the observation portion observation and carries sample piece, gathers sample parameter.

Further, the observation portion of the optical observation section of this scheme includes the slide glass, is equipped with the coverslip on the slide glass, and fixed pressing is equipped with fixed frame on the coverslip, and fixed frame withholds the coverslip on the slide glass, and outside inspection equipment observes the sample on the slide glass, gathers sample parameter through the coverslip.

Further, the optical observation section of this scheme is confined structure, is equipped with on the position that observation portion corresponds and carries the appearance piece, and the outside inspection equipment passes through the sample on the observation portion observation and carries appearance piece, gathers sample parameter.

Further, be equipped with the fluid-discharge tube body on the outside of the test tube body of this scheme, be equipped with on the fluid-discharge tube body with the liquid taking through-hole of test tube body's optics observation section intercommunication, with establish the flowing back through-hole of test paper reaction unit intercommunication on the fluid-discharge tube body outside, be equipped with the flowing back guide pillar in the fluid-discharge tube body, the flowing back guide pillar includes cut-off section, the section that leads to, the external diameter of flow-through section is less than the external diameter of cut-off section, cut-off section and the inside wall zero clearance sliding contact of fluid-discharge tube body, the inside wall that leads to the flow-through section and the fluid-discharge tube body forms the flowing back ring way, with liquid taking through-hole, flowing back through-hole and flowing back ring way intercommunication, promote the test tube stopper to let into test paper reaction unit with the sampling liquid from liquid taking through-hole, flowing back ring way, flowing back through-hole.

Furthermore, a reset spring is arranged between the liquid discharging guide post and the inner bottom of the liquid discharging pipe body, the liquid discharging guide post is driven to communicate the liquid taking through hole, the liquid discharging through hole and the liquid discharging loop to finish liquid discharging operation, and the liquid discharging guide post resets under the action of the reset spring.

Further, be equipped with the drainage groove on the outside of the test tube body of this scheme, be equipped with the liquid through-hole of getting on the drainage groove, be equipped with the flowing back slider in the drainage groove, be equipped with the flowing back through-hole on the flowing back slider, with flowing back through-hole and liquid through-hole intercommunication of getting, promote the test tube stopper and follow liquid through-hole, flowing back through-hole and arrange into the test paper reaction unit who fixes on the flowing back slider with the sampling liquid.

Still further, the test paper reaction subassembly of this scheme includes rectangular groove base plate, is equipped with the test paper picture peg in the slot of rectangular groove base plate, is equipped with the test paper in the test paper picture peg, takes place to detect chemical reaction from flowing back through-hole exhaust sample liquid and test paper.

Still further, the strip-shaped groove substrate of the scheme is of a cuboid structure, the long side wide surface A of one side of the strip-shaped groove substrate is open, the short side narrow surface A of one side adjacent to the long side wide surface A is open, and the test strip plugboard is inserted into the strip-shaped groove substrate from the short side narrow surface A

Inside, be equipped with a plurality of bellied button head stopper structures in long side broad A on long side broad A's the opening border, button head stopper structure prevents that the test paper strip picture peg from dropping from long side broad A.

Still further, the long side broad face C opening of this scheme's test paper strip picture peg one side, the test paper strip is put into the test paper strip picture peg inside from long side broad face C, is equipped with a plurality of bellied button head stopper structures to the test paper strip picture peg inside on long side broad face C's the opening inboard border, and button head stopper structure prevents that the test paper strip from dropping from long side broad face C.

Still further, the test paper reaction subassembly of this scheme includes rectangular shape groove base plate, be equipped with the test paper groove in the slot of rectangular shape groove base plate, test paper groove bottom is equipped with reaction circle through-hole A, the long through-hole A of reaction, be equipped with the test paper in the test paper groove, test paper groove top in the rectangular shape groove base plate is equipped with the test paper clamp plate, be equipped with reaction circle through-hole B on the test paper clamp plate, the long through-hole B of reaction, reaction circle through-hole B corresponds with reaction circle through-hole A, reaction long through-hole B corresponds with the long through-hole A of reaction, follow flowing back through hole exhaust sample liquid entering reaction circle through-hole B, reaction long through-hole B takes place to detect chemical reaction with the test paper.

Further, be equipped with L shape fluid-discharge tube on the outside of this scheme's test tube body, the one end and the optics observation section intercommunication of L shape fluid-discharge tube are equipped with flowing back control cap on the other end of L shape fluid-discharge tube, promote the test tube stopper and follow the other end of L shape fluid-discharge tube and discharge into test paper reaction unit with the sampling liquid.

Furthermore, the other end of the L-shaped liquid discharge pipe is of a pipe structure with the outer diameter gradually reduced along the liquid discharge direction, the liquid discharge control cap is pushed along the release direction to form limit contact with the cap stop block arranged on the outer side of the test tube body, and the sampling liquid is discharged along the gap between the other end of the L-shaped liquid discharge pipe and the inner side of the liquid discharge control cap.

Still further, the end portion of the other end of the L-shaped liquid discharge pipe is of a closed structure, a liquid discharge hole is formed in the outer side of the other end of the L-shaped liquid discharge pipe and is communicated with an optical observation section through an inner cavity of the L-shaped liquid discharge pipe, a liquid discharge control cap is pushed in a disengaging direction to form limiting contact with a cap stop block arranged on the outer side of a test tube body, and sampling liquid is discharged from the liquid discharge hole.

Still further, the cover is equipped with the spring on the L shape fluid-discharge tube other end of this scheme, and the spring presses the cap dog that forms spacing contact on the cap on the test tube body outside with flowing back control cap, and the end portion of L shape fluid-discharge tube other end is enclosed construction, is equipped with the apopore on the outside of L shape fluid-discharge tube other end, promotes flowing back control cap to overcome the stress opening apopore of spring, and the sampling liquid is discharged from the apopore.

Further, the liquid discharge nozzle is arranged at the far end of the optical observation section, the liquid discharge control cap is sleeved on the liquid discharge nozzle, and the test tube plug is pushed to discharge the sampling liquid from the liquid discharge nozzle into the test paper reaction assembly.

The integrated multifunctional biological sample processor provided by the invention realizes the integration of various biological sample processing functions by adopting the integrated processor, and has the characteristics of convenience in operation and high efficiency.

Drawings

FIG. 1 is a block diagram of an integrated multifunctional biological sample processor of the present invention.

FIG. 2 is a cross-sectional view of the test tube body along the flow direction of the sample liquid.

Fig. 3 is an assembly structure diagram of the test tube plug and the sampling square spoon.

Fig. 4 is a structural view of the sampling baffle a.

Fig. 5 is a structural view of the sampling barrier B.

Fig. 6 is a block diagram of a closed flow state of the sampling combination barrier.

Fig. 7 is a block diagram of the sample assembly baffle in an open flow state.

Fig. 8 is an assembly structure diagram of the drainage guide post and the return spring.

FIG. 9 is a schematic diagram of the test tube and the drain tube.

Fig. 10 is a block diagram of a first embodiment of an elongated slot substrate.

Fig. 11 is a structural view of the test strip insertion plate.

FIG. 12 is a schematic view of a test tube and a drain slider.

FIG. 13 is a schematic view of a drain groove on the outside of the test tube body.

Fig. 14 is a structural view of the liquid discharge slider.

Fig. 15 is a structural diagram of a second embodiment of an elongated slot substrate.

Fig. 16 is a structural view of the test strip pressing plate.

FIG. 17 is a schematic diagram of a test tube and an L-shaped drain.

FIG. 18 is a view showing a constitution of a first embodiment of a test tube body and an L-shaped liquid discharge tube.

FIG. 19 is a schematic view showing a constitution of a test tube and an L-shaped liquid discharge tube according to a second embodiment.

FIG. 20 is a schematic view of a third embodiment of a test tube and an L-shaped drain.

Wherein 100 is a test tube body, 101 is a sample carrying sheet, 110 is a sampling processing section, 120 is an optical observation section, 121 is an observation section, 130 is a test tube plug, 131 is a sealing ring, 132 is a liquid discharge piston, 140 is a sampling square spoon, 150 is a sampling baffle a,151 is a slope-shaped flow guiding surface, 160 is a sampling baffle B,170 is a sampling combination baffle, 171 is a baffle a,172 is a baffle B,173 is a baffle c,174 is a connecting rod, 175 is a guide ring, 176 is an elastic section, 200 is a liquid discharge tube body, 210 is a liquid discharge through hole, 220 is a liquid discharge guide post, 221 is a cut-off section, 222 is a through-flow section, 230 is a return spring, 240 is a liquid discharge groove, 250 is a liquid discharge slider, 300 is a test paper reaction assembly, 301 is a round head limiting block structure, 310 is a strip-shaped groove substrate, 320 is a test strip insertion plate, 331 is a test strip groove, 332 is a reaction round through hole a,340 is a reaction round through hole a, 341 is a reaction round through hole B,342 is an L-shaped liquid discharge tube, 411 is a hole, 420 is a control cap, 430 is a stop, and 440 is a stop.

Detailed Description

As shown in fig. 1 and 2, the integrated multifunctional biological sample processor of the invention comprises a test tube body, the test tube body comprises a sampling treatment section and an optical observation section, the sampling treatment section is communicated with the optical observation section to form a whole test tube body, a test tube plug is arranged on an upper cover of a test tube body inlet at one end of the sampling treatment section, the optical observation section comprises an observation part, a biological sample entering the sampling treatment section is processed to form a sampling liquid, and the sampling liquid enters the optical observation section, and external inspection equipment observes a sample and acquires sample parameters through the observation part. Further, the partial side wall of the sampling processing section of the scheme is of a cylindrical surface structure, the partial side wall of the observation part of the optical observation section is of a plane structure, the cylindrical surface structure and the plane structure are connected through an inclined plane structure to form the whole partial side wall of the test tube body, and the external inspection equipment observes samples and collects sample parameters through the plane structure. The above-mentioned scheme adopts the multiple functions such as integration sampling processing, sampling observation of integration processor realization, and wherein, the improvement of test tube body structure is favorable to adjusting the capacity of test tube, is convenient for external equipment observe, inspection sampling simultaneously to biological sample inspection's operating efficiency has been improved by a wide margin.

In order to realize the liquid draining function of the test tube plug, the scheme discloses the following specific embodiments.

Example 1

As shown in fig. 3, the test tube plug of the scheme is a columnar structure with the appearance matched with the inner side of the inlet of the test tube body, a plurality of raised sealing rings extending along the circumferential direction are arranged on the outer side of the columnar structure, the test tube plug is pushed to be tightly covered, sampling liquid is discharged into the test paper reaction assembly, and the sealing rings prevent the biological sample in the test tube body from being infiltrated outwards.

Example two

The test tube plug is fixedly covered on the inlet, an elastic hollow ball is arranged at the outer end of the test tube plug, and the elastic hollow ball is extruded to discharge sampling liquid into the observation part. The test paper reaction component can be pressed into the test paper reaction component by adopting a rubber hollow ball (liquid draining air bag) communicated with the test paper body and increasing the air pressure in the test paper body by extruding the liquid draining air bag by an operator.

Example III

As shown in fig. 2, the test tube stopper comprises a test tube stopper cavity, the test tube stopper cavity is fixed in the inlet of the test tube body, a liquid draining piston is arranged on the part of the inlet side of the test tube body, and the liquid draining piston is pushed to drain the sampling liquid into the test paper reaction component. The liquid draining piston forms an air pressure effect with the test tube body through the cavity of the test tube plug, and the sampling liquid is hydraulically pressed into the test paper reaction assembly.

Example IV

The test tube plug is fixedly covered tightly with the inlet, a columnar quantitative liquid discharge piston is arranged at the outer end of the test tube plug, a liquid discharge scale is arranged on the outer side of the columnar quantitative liquid discharge piston along the liquid discharge direction, and the columnar quantitative liquid discharge piston is pushed to discharge sampling liquid into the test paper reaction component according to the liquid discharge scale.

In order to realize the biological sample sampling operation function, the following specific embodiments are disclosed.

Example 1

As shown in fig. 3, the test tube stopper of this scheme is equipped with the square spoon of sample on the internal one end of test tube, and the square spoon of sample includes spoon head, spoon handle, and the spoon handle is tubular structure, and the square spoon of sample is established on the connecting handle of test tube stopper on one end in the test tube through spoon handle cover. For the convenience of stirring and evenly mixing the sample, a plurality of through hole structures are arranged on the spoon head of the sampling Fang Shao in the scheme.

Example two

In order to realize that sampling liquid or objects meeting requirements are screened and discharged into an observation part so as to be convenient for observation and detection, the scheme discloses the following specific embodiments.

Example 1

As shown in fig. 2 and 4, a sampling baffle a is arranged in the rear section of the sampling treatment section of the test tube body in the scheme, a circulation gap is arranged between the sampling baffle a and the inner side wall of the sampling treatment section, the sampling baffle a comprises a slope-shaped guide surface, and a biological sample in the sampling treatment section is centrifugally treated and then deposited to an observation part of the optical observation section through the slope-shaped guide surface. The slope-shaped flow guide surface is beneficial to the precipitation of components such as cells and the like from the flow guide surface to a counting pool area at the tail part of the test tube during the centrifugation of the sample.

Example two

As shown in fig. 2 and 5, a sampling baffle B is disposed in the rear section of the sampling treatment section of the test tube body in this scheme, the sampling baffle B is tightly combined with the inner sidewall of the sampling treatment section along the circumferential direction, a plurality of filtering through holes are disposed on the sampling baffle B, and the biological sample in the sampling treatment section enters the observation portion of the optical observation section after being filtered by the filtering through holes. The filter holes can filter out unwanted impurities or components according to the set size.

Example III

As shown in fig. 2, 6 and 7, a sampling combined baffle is arranged in the rear section of the sampling treatment section of the test tube body, the sampling combined baffle is in close contact with the inner side wall of the sampling treatment section along the circumferential direction, the sampling combined baffle comprises a baffle a, a baffle b and a baffle c which are connected in sequence to form the whole sampling combined baffle through an elastic part, a connecting rod connected with a test tube plug is arranged on the baffle b, the inner end of the connecting rod is movably sleeved in a fixed guide ring, the distal side edge of the baffle a is connected with the guide ring through the elastic part, the distal side edge of the baffle c is connected with the guide ring through the elastic part, the pushing test tube plug linkage connecting rod pushes the baffle b to enable the sampling combined baffle to deform and fold up to form a circulation gap with the inner side wall of the sampling treatment section, a biological sample in the sampling treatment section enters the test paper reaction assembly through the circulation gap, the test tube plug is withdrawn, and the sampling combined baffle resets under the action of the elastic part. The sampling combined baffle is opened while the liquid discharging piston discharges liquid, so that the sampling liquid can enter the observation part conveniently.

In order to facilitate equipment such as an external microscope to observe a sample and realize sampling operation, the following specific embodiments are disclosed.

Example 1

As shown in fig. 2,4 and 5, a sampling baffle is arranged in a sampling treatment section of the test tube body in the scheme, one end of the sampling baffle extends into an observation portion of an optical observation section to form a sample carrying sheet, and external inspection equipment observes samples on the sample carrying sheet through the observation portion and collects sample parameters.

Example two

As shown in fig. 12, the observation portion of the optical observation section of the present embodiment includes a sample slide, a cover glass is provided on the sample slide, a fixing frame is fixedly pressed on the cover glass, the cover glass is buckled on the sample slide by the fixing frame, and an external inspection device observes a sample on the sample slide and collects parameters of the sample through the cover glass.

Example III

The optical observation section of this scheme is confined structure, is equipped with on the position that observation portion corresponds and carries the appearance piece, and the outside inspection equipment passes through the sample on the observation portion observation carries the appearance piece, gathers sample parameter.

In order to realize the purpose of leading the sampling liquid out of the test tube body, the scheme discloses the following specific embodiments.

Example 1

As shown in fig. 9, the liquid draining tube body is arranged on the outer side of the test tube body, the liquid taking through hole communicated with the optical observation section of the test tube body is arranged on the liquid draining tube body, the liquid draining through hole communicated with the test paper reaction assembly arranged on the outer side of the liquid draining tube body is arranged in the liquid draining tube body, the liquid draining guide post comprises a stop section and a through flow section, the outer diameter of the through flow section is smaller than that of the stop section, the stop section is in clearance-free sliding contact with the inner side wall of the liquid draining tube body, the through flow section and the inner side wall of the liquid draining tube body form a liquid draining ring channel, the liquid taking through hole, the liquid draining through hole and the liquid draining ring channel are communicated, and the test paper plug is pushed to drain sampling liquid from the liquid taking through hole, the liquid draining ring channel and the liquid draining through hole into the test paper reaction assembly.

Example two

On the basis of the scheme, a reset spring is arranged between the liquid discharge guide pillar and the inner bottom of the liquid discharge pipe body, as shown in fig. 8, the liquid discharge guide pillar is driven to communicate the liquid taking through hole, the liquid discharge through hole and the liquid discharge loop to finish liquid discharge operation, and the liquid discharge guide pillar resets under the action of the reset spring.

Example III

As shown in fig. 12, 13 and 14, a liquid draining groove is arranged on the outer side of the test tube body, a liquid taking through hole is arranged on the liquid draining groove, a liquid draining sliding block is arranged in the liquid draining groove, the liquid draining sliding block is provided with a liquid draining through hole, the liquid draining through hole is communicated with the liquid taking through hole, and the test tube plug is pushed to drain sampling liquid from the liquid taking through hole and the liquid draining through hole into a test paper reaction component fixed on the liquid draining sliding block.

Example IV

The scheme that the liquid outlet is directly formed in the observation section can also be adopted, namely, the liquid outlet is formed in the far end of the optical observation section in the scheme, a liquid outlet control cap is sleeved on the liquid outlet, and the sampling liquid is pushed to be discharged into external inspection equipment from the liquid outlet by the test tube plug. The liquid discharge control cap is used for controlling the discharge of the sampling liquid.

Example five

As shown in fig. 17, an L-shaped liquid discharge tube is arranged on the outer side of the test tube body in the scheme, one end of the L-shaped liquid discharge tube is communicated with the optical observation section, a liquid discharge control cap is arranged on the other end of the L-shaped liquid discharge tube, and the test tube plug is pushed to discharge sampling liquid from the other end of the L-shaped liquid discharge tube into the test paper reaction assembly.

As shown in fig. 18, on the basis of the scheme of fig. 17, the other end of the L-shaped liquid discharge tube of the scheme is of a tube structure with the outer diameter gradually reduced along the liquid discharge direction, the liquid discharge control cap is pushed along the disengaging direction to form limit contact with the cap stop block arranged on the outer side of the test tube body, and the sampling liquid is discharged along the gap between the other end of the L-shaped liquid discharge tube and the inner side of the liquid discharge control cap.

As shown in fig. 19, on the basis of the scheme of fig. 17, the end head of the other end of the L-shaped liquid discharge tube is of a closed structure, a liquid discharge hole is formed in the outer side of the other end of the L-shaped liquid discharge tube, the liquid discharge hole is communicated with the optical observation section through an inner cavity of the L-shaped liquid discharge tube, a liquid discharge control cap is pushed in a disengaging direction to form limit contact with a cap stop block arranged on the outer side of the test tube body, and sampling liquid is discharged from the liquid discharge hole.

As shown in fig. 20, on the basis of the scheme of fig. 17, a spring is sleeved on the other end of the L-shaped liquid discharge tube in the scheme, the spring presses the liquid discharge control cap against a cap stop block on the outer side of the test tube body to form limit contact, the end head of the other end of the L-shaped liquid discharge tube is of a closed structure, a liquid discharge hole is formed in the outer side of the other end of the L-shaped liquid discharge tube, the liquid discharge control cap is pushed to overcome the stress of the spring to open the liquid discharge hole, and sampling liquid is discharged from the liquid discharge hole.

In order to realize the reaction of the sample liquid leading-in test paper, the scheme also discloses the following specific embodiments of the test paper reaction assembly.

Example 1

As shown in fig. 10 and 11, the test paper reaction component of the present embodiment includes a substrate with a long strip-shaped groove, a test strip insertion board is disposed in a slot of the substrate with a test strip, and a test strip is disposed in the test strip insertion board, and a sample liquid discharged from a liquid discharge through hole and the test strip undergo a chemical reaction.

On the basis of the scheme, the strip-shaped groove substrate of the scheme is of a cuboid structure, the long side wide surface A of one side of the strip-shaped groove substrate is open, the short side narrow surface A of one side adjacent to the long side wide surface A is open, the test strip inserting plate is inserted into the strip-shaped groove substrate from the short side narrow surface A, a plurality of round head limiting block structures protruding into the long side wide surface A are arranged on the opening edge of the long side wide surface A, and the round head limiting block structures prevent the test strip inserting plate from falling from the long side wide surface A. Similarly, the long side broad face C opening of test paper strip picture peg one side of this scheme, the test paper strip is put into inside the test paper strip picture peg from long side broad face C, is equipped with a plurality of bellied button head stopper structures to test paper strip picture peg inside on long side broad face C's the inboard border of opening, and button head stopper structure prevents that the test paper strip from dropping from long side broad face C.

Example two

As shown in fig. 15 and 16, the test paper reaction component of the scheme comprises a strip-shaped groove substrate, a test paper strip groove is arranged in a slot of the strip-shaped groove substrate, a reaction round through hole a and a reaction long through hole a are arranged at the bottom of the test paper strip groove, test paper strips are arranged in the test paper strip groove, a test paper strip pressing plate is arranged above the test paper strip groove in the strip-shaped groove substrate, a reaction round through hole B and a reaction long through hole B are arranged on the test paper strip pressing plate, the reaction round through hole B corresponds to the reaction round through hole a, the reaction long through hole B corresponds to the reaction long through hole a, and sampling liquid discharged from a liquid discharge through hole enters the reaction round through hole B, and the reaction long through hole B and the test paper strips are subjected to detection chemical reaction. The reaction condition of the test paper can be observed through the reaction round through hole A and the reaction long through hole A.

The scheme discloses a collection sampler, a loading test tube, a filtering and isolating baffle plate, an optical observation area counting pool, a liquid discharging opening and extruding device and a test strip chemical reaction plate. The technical scheme includes that the device comprises a test tube, a counting pool (optical observation section), a sampling spoon (sampler), a baffle plate (baffle plate or a drain screen) in the tube, a piston (a tube cover or a tube plug), a liquid discharge switch device, a test strip chemical reaction plate and other components, wherein the components are connected into a whole in an inner cavity container which is mutually communicated. The counting pool (optical observation section) is communicated with the inner cavity of the test tube, is a sealing structure with an interlayer, a convex plane (sample carrying sheet) is arranged in the middle of the optical observation area, and a groove is arranged on the edge. The middle convex plane is an optical observation area, and the grooves are beneficial to flowing liquid into the middle optical observation area and are beneficial to processing and manufacturing. The baffle in the pipe is provided with a half baffle, a net baffle and an umbrella baffle. The baffle plate is used for separating and filtering the liquid sample. The half gear has a plate slope structure, so that effective components in the sample can enter a counting cell, and particularly during centrifugation. The mesh baffle plate is provided with a small hole structure to filter out unwanted components in the sample. The umbrella-shaped baffle (sampling combined baffle) is provided with an inner pipe and an outer pipe, 2 semicircular baffles are connected with the outer pipe through elastic foldable connecting sheets, the baffles are folded and unfolded through pushing and pulling the outer pipe, liquid in the test tube is communicated during folding, and sample liquid is separated during opening. The liquid discharge switch device (liquid discharge tube body) is provided with a mechanism for opening and closing a liquid discharge small hole on the test tube. The piston (cap or plug) has a mechanism for squeezing out the liquid in the tube. The test paper strip chemical reaction plate is clamped on the side surface of the liquid discharge piston through a clamping groove, a hole communicated with the liquid discharge piston hole is formed in the side surface of the liquid discharge piston, and a small plug board with a chemical test paper strip is inserted in the middle of the hole. By opening the drain switch (drain guide post), the piston is pushed to drain the liquid sample in the test tube into the test strip chemical reaction plate or other container.

In view of the complicated steps of the existing scheme in the operation process, the scheme provides a biological sample processor which does not need a pipette gun, a pipette needle, a peristaltic pump and a liquid pipeline, is time-saving and labor-saving, is low in manufacturing cost and has less pollution than an integral body, can realize microscopic morphological examination without transferring liquid, realizes liquid transfer to a test strip chemical reaction plate or other containers through a liquid discharge switch device, realizes liquid layering through a baffle, and simultaneously has a sampling tool and a container space for sample processing. The test tube inner chamber and the counting cell inner chamber of this scheme are a whole that communicates, and the treater is the bottom parallel, and the upper portion is half arc test tube body, and the afterbody is the counting cell (optical observation section) that has the cavity in the partial flat, and both sides face or bottom can adorn flowing back switching device (flowing back body or groove piece mechanism), and flowing back switching device side can insert test paper strip chemical reaction board (test paper reaction subassembly). A baffle plate can be inserted into the inner cavity of the test tube body. The front half part of the pipe plug is provided with a plurality of raised leakage preventing lines, and the front half part can be inserted with a sampling spoon handle or a spoon to be integrally formed on the pipe plug.

Several counter cell configurations are shown in figures 2,4, 5, 12. The 1 st structure is that the upper and lower surfaces of the tail part of the test tube are flat interlayer cavities, the front half sheet of the baffle plate is inserted into the counting tank, the thickness of the sheet is smaller than the height of the cavity of the counting tank, the width is smaller than the width of the inner cavity of the counting cell, so that the sample carrying sheet with the convex plane in the middle and the concave groove on the edge is formed. The 2 nd structure is that the tail part of the test tube is provided with a convex plane (sample carrying sheet) in the middle, the edge is provided with a groove, four corners are provided with four small holes, a cover glass is covered on a base plate (sample carrying sheet), the cover glass is buckled and pressed on the base plate by using a frame-shaped pressing strip, and four pressing strip column tips of the frame-shaped pressing strip are pressed into the four small holes of the base plate. The 3 rd structure is integrated, namely the counting cell and the test tube body are formed at one time, the upper surface and the lower surface of the tail part of the test tube are flat interlayer inner cavities, the lower surface of the tail part is provided with a convex plane (sample carrying sheet), and the edge of the tail part is provided with a groove counting cell. The counting cell material with the above 3 structures is a plastic material with high transparency close to optical glass, and the cover glass in the 2 nd structure can also be a glass quartz material.

Several drain switch arrangements are shown in figures 8, 9, 12, 13, 14. The 1 st structure, the side of the test tube body is a cylindrical cavity (drain tube body) into which a cylindrical drain hole piston (drain guide post) with alternate thickness is inserted, a spring is arranged below the cylindrical cavity, two corresponding holes are arranged on two sides of the inner wall of the cylindrical cavity, one side hole is arranged on the side of the test tube body, the holes are communicated with the inner cavity of the test tube body, and the other side hole is arranged on the side of the cylindrical cavity and communicated with the outside. When the spring is sprung, the two side holes are just plugged at the position of the liquid outlet piston, the liquid outlet piston is pressed from the pipe orifice, the spring is compressed, the thin part of the liquid outlet piston faces the two side holes, and the thin part of the liquid outlet piston is communicated with the 2 side holes through a gap. Thus, the liquid outlet of the test tube body can realize the switch action. The 2 nd structure, the side of the test tube body is a cylindrical cavity, a liquid outlet piston with alternate thickness is inserted into the cylindrical cavity, no spring is arranged below the cylindrical cavity, two corresponding holes are arranged on two sides of the inner wall of the cylindrical cavity, one side hole is arranged on the test tube body side, the holes are communicated with the inner cavity of the test tube body, and the other side hole is arranged on the side of the cylindrical cavity and is communicated with the outside. When the liquid outlet piston is pulled to the outermost surface, the thick part of the rod body just plugs the two side holes, and when the liquid outlet piston is pushed inwards from the pipe orifice to the bottommost surface, the thin part of the liquid outlet piston faces the two side holes, and the thin part of the liquid outlet piston is communicated with the 2 side holes through a gap, so that the liquid outlet of the test tube body can realize the switching action. In the 3 rd structure, the side surface of the test tube body is provided with a circular long groove (liquid discharge groove), the middle part of the long groove hook is provided with a small hole which is communicated with the inner cavity of the test tube, a convex long circular chuck on a liquid discharge valve (liquid discharge sliding block) can be sleeved into the groove on the test tube body, and the liquid discharge valve is provided with a liquid discharge port, a positioning pin and a groove hanging hole. When the push-pull liquid draining valve is pushed forward to the left positioning pin to block, the small hole on the test tube body is blocked and closed by the liquid draining valve, and when the push-pull liquid draining valve is pulled backward to the right positioning pin to block, the small hole on the test tube body and the small hole on the liquid draining valve are opened in opposite directions, so that the liquid draining port of the test tube body realizes the opening and closing action. The 4 th structure, the optical observation area (section) of the test tube body is provided with a convex liquid discharge nozzle, the liquid discharge nozzle can be sleeved with a cap, the cap is taken down when liquid discharge is needed, the liquid discharge piston is pushed, and the cap is not needed to be covered when liquid discharge is needed. Besides the above 4 structures, the structure is also in various modes such as a plug type structure and the like, so that the opening and closing actions of the liquid outlet of the inner cavity of the test tube body are realized, the sealing is good, and the manual and mechanical automation operation is convenient. The liquid outlet piston and the liquid outlet valve component with the structures are made of soft elastic plastic materials, so that the sealing effect is facilitated.

Several liquid discharge nozzle switch structures are shown in fig. 17-20. The optical observation section is provided with an L-shaped liquid discharging nozzle, the front section of the L-shaped liquid discharging nozzle is thin, the rear section of the L-shaped liquid discharging nozzle is thick, the center of the L-shaped liquid discharging nozzle is provided with a pipe hole communicated with a test tube body, a liquid discharging control cap is sleeved on the liquid discharging nozzle, one surface of the cap, which is close to the test tube body, of the cap is flat, the side surface, which is close to the test tube body, of the cap, a cap baffle is arranged on the test tube body, when the cap is pushed to the baffle, a part of the thin section of the L-shaped liquid discharging nozzle is exposed, a gap channel is formed between the pipe hole and the thin section of the liquid discharging nozzle, and the test tube plug is pushed to discharge sampling liquid from the liquid discharging nozzle into external inspection equipment. When the liquid discharge nozzle is opened, the liquid discharge control cap is not required to be taken up from the test tube body and is sleeved on the liquid discharge nozzle column. The second optical observation section is provided with an L-shaped liquid discharging nozzle, the front section of the L-shaped liquid discharging nozzle is solid, the rear section of the L-shaped liquid discharging nozzle is a hollow pipe body, a small hole is formed in the hollow part at the joint of the solid section and the hollow section, a liquid discharging control cap is sleeved on the liquid discharging nozzle, one surface of the cap, which is close to the test pipe body, is flat, the side surface, which is close to the test pipe body, of the cap is also flat, the test pipe body is provided with a cap baffle, and when the cap is pushed to the baffle, the small hole of the thin hollow section of the L-shaped liquid discharging nozzle is just exposed to push a test pipe plug to discharge sampling liquid from the liquid discharging nozzle into external inspection equipment. When the liquid discharge nozzle is opened, the liquid discharge control cap is not required to be taken off from the test tube body and is sleeved on the liquid discharge nozzle column. The side of the optical observation section is provided with an L-shaped liquid discharging nozzle, the front section of the L-shaped liquid discharging nozzle column is sleeved with a spring, the rear section of the L-shaped liquid discharging nozzle column is sleeved with a liquid discharging control cylinder sleeve, the inner wall of the liquid discharging control cylinder sleeve is in seamless cling with the L-shaped liquid discharging nozzle column, a liquid discharging small hole is formed near a liquid discharging nozzle hole, a blocking cover is inserted into the liquid discharging nozzle hole, the surfaces of the cylinder sleeve and the blocking cover, which lean against the test tube body, of the test tube are flat, the center of the liquid discharging nozzle is provided with a pipe hole communicated with the test tube body, the test tube is tightly clung to the side surface of the test tube body by the cylinder sleeve and the blocking cover, the liquid discharging small hole is exposed by pressing the liquid discharging control cylinder sleeve, and the test tube plug is pushed to discharge sampling liquid from the liquid discharging nozzle into external inspection equipment. The liquid discharge opening function is realized by only pressing the liquid discharge control cylinder sleeve, so that the liquid discharge small hole is exposed, and the spring liquid discharge control cylinder sleeve is loosened to reset so as to block and close the liquid discharge small hole.

The scheme also comprises a plurality of liquid discharging piston structures, wherein the liquid discharging piston structures comprise a test tube plug with a sealing ring, a test tube plug with an air bag, a cavity with a certain capacity, an exhaust hole communicated with the inner cavity of the test tube, a liquid discharging piston sleeved outside the test tube plug, and a small liquid discharging piston (a columnar quantitative liquid discharging piston) with scales. The liquid discharge piston component with the 4 structures is made of soft elastic plastic material, and is favorable for sealing. When the liquid discharge hole is opened, the liquid discharge piston is pushed inwards to advance, the pressure in the test tube is increased, the sample is extruded out of the test tube through the liquid discharge hole, and the sample is dripped into a test strip chemical reaction plate (test strip reaction assembly) or other containers.

As shown in the spoon structure shown in FIG. 3, the spoon body consists of a pipe plug, a spoon handle and a spoon, the three parts are spliced by a mounting hole sleeve, and the spoon is provided with small holes, so that the stirring and uniformly mixing of samples are facilitated. The spoon structure can also be a non-porous structure, and the shape can also be round, grooved hole-shaped, bar-shaped (the appearance of the bar is spiral, or pricked), etc.

Several baffle structures are shown in fig. 2, 4, 5, 6, 7. The semi-baffle plate consists of a slope-shaped guide surface, a positioning hole and a thin sheet. The slope-shaped diversion surface is favorable for the components such as cells and the like to be precipitated from the diversion surface to a counting pool area at the tail part of the test tube when the sample is centrifuged, the locating hole is matched with the locating column in the inner cavity of the test tube to locate the baffle plate at the fixed position of the test tube, and the lamellar sheet is inserted into the optical observation area of the test tube to serve as a convex plane (sample carrying sheet). The baffle plate of the semi-baffle plate is about half of the height of the inner cavity of the test tube, the inner cavity of the test tube is communicated, and when the optical observation area of the test tube is placed in parallel under a microscope for observation, the components such as sediment cells at the tail of the centrifuged test tube are blocked at the side of the counting pool (observation part). The full baffle plate with filtering holes consists of filtering hole surfaces, positioning holes and thin sheets. The surface of the filtering holes can filter out large impurities or unwanted components, and the size of the mesh can be designed according to the components to be filtered. The locating hole cooperates with the locating column in the test tube inner chamber to locate the baffle in the test tube fixed position, the lamellar sheet inserts the test tube optical observation area as the protruding plane (carrying the sample sheet). The baffle plate of the full baffle plate of the filtering hole type is identical to the inner cavity of the test tube in height, and the inner cavity of the test tube is communicated with the filtering hole. The umbrella type baffle (sampling combined baffle) consists of a baffle main body, a guide sleeve, a test tube cover and an operating handle. Like umbrella-shaped inner and outer tube structures, the operating handle is an inner tube, the guide sleeve (outer tube) is sleeved outside the operating handle, the baffle main body consists of two semicircular plates (baffle a and baffle c), and the size and shape of the two semicircular plates are the same as the cross-section size and shape of the inner cavity of the test tube. The two semicircular baffle plates of the baffle body are respectively provided with 1 elastic soft sheet (elastic part) which is connected with the guide sleeve, and the guide sleeve and the test tube cover (plug) are integrated. The operating handle is pinched by one hand, the test tube cover is pressed inwards by one hand, the umbrella-shaped baffle plate is opened by the two semicircular baffle plates, the test tube cover is pulled outwards, and the two semicircular baffle plates are closed. By the operation, the baffle is opened and closed, the inner cavity of the test tube is communicated when the baffle is opened, and the inner cavity of the test tube is divided into two closed cavities when the baffle is closed. In view of the many blood testing projects that require the centrifuged serum, the upper serum and the lower blood sediment components can be separated by umbrella type baffles.

The structure of the strip chemical reaction plate (strip reaction module) as shown in fig. 1, 10, 11, 15, 16. The test strip chemical reaction plate consists of a main plate body (a strip-shaped groove base plate) and a test strip small plugboard. The side of the test strip chemical reaction plate is provided with a convex cylindrical groove head, the cylindrical groove head is sleeved into a groove on the side of the liquid discharge piston, the two grooves are spliced together, the holes on the spliced test strip chemical reaction plate are aligned with the holes of the liquid discharge piston, the holes are communicated, the middle of the test strip chemical reaction plate is provided with a long groove, a small inserting plate with test strips can be inserted into the test strip chemical reaction plate, the small inserting plate of the test strip has inserting plates with various sizes according to different test strip sizes in the market, and two sides of the middle inserting groove of the small inserting plate are respectively provided with a test strip inserting plate clamping groove for fixing the test strip, and the test strip is fixed in the middle groove of the small inserting plate.

The biological sample processor of the scheme is mainly used for single microscopic morphological examination and combined microscopic morphological examination and test strip chemical project examination. The following classification describes.

The processor comprises a test tube body, an optical observation area, a test tube plug (cover), a spoon and a baffle.

Example 1

And (5) checking urinary sediment. The baffle is a half baffle, and a spoon is not needed. Adding urine into a test tube, plugging a test tube stopper on a test tube port, centrifuging in a centrifuge, centrifuging cell sediment to a test tube optical observation area, and then inserting the test tube optical observation area (a counting tank and an observation part) into a microscope for observation form and counting, wherein a semi-baffle plate is used for enabling the cell sediment in the optical observation area not to flow back into a supernatant of the test tube when the test tube is horizontally placed on a microscope object stage.

Example two

And (5) stool examination. A filtering hole type baffle plate is selected, a spoon is needed, and diluent is added in advance when a test tube leaves a factory. The stool is sampled and inserted into the test tube plug, the test tube plug is tightly plugged by the spoon, the spoon and the test tube plug are integrated, the oscillator is put into the spoon to mix the sample uniformly, the spoon meshes are favorable for mixing the stool sample uniformly, and then the optical observation area of the test tube is inserted into the microscope to observe the shape and count.

Example III

Examination of gynecological leucorrhea secretion. The same procedure as in the example was followed except that the spoon was replaced with a cotton swab (the swab was mounted on the plug) and the leucorrhea secretion was removed with the cotton swab.

Example IV

Blood examination. An umbrella-shaped baffle plate is selected, a spoon is not needed, and anticoagulation or other treatment liquid is added in advance when the test tube leaves a factory. Adding blood into a test tube, plugging the test tube mouth with a tube plug, putting the umbrella-shaped baffle plate in a folded state, centrifuging the cell sediment in a centrifugal machine, centrifuging the cell sediment in an optical observation area of the test tube, opening the umbrella-shaped baffle plate after centrifuging, separating the upper serum from the lower cell sediment of the test tube by the baffle plate, and checking the upper serum as a project requiring a serum sample, wherein the optical observation area is inserted into a microscope for checking the observation form and counting.

Example five

Other cells, bacteria, microorganisms were directly biopsied and stained for examination. According to the requirements of different samples and inspection items, different treatment liquids or no treatment liquid are added in advance in a test tube, different sampling tools are inserted in a tube plug, centrifugation or no centrifugation is carried out, dyeing liquid or no dyeing liquid is added, and a baffle plate is optionally matched, so that the samples in an optical observation area are suitable for the inspection requirements of the items after combined assembly is carried out.

The processor comprises a test tube body, an optical observation area, a test tube plug (cover), a spoon, a baffle plate, a liquid discharge switch device and a test strip chemical reaction plate.

Example six

Urine dry chemistry analysis is performed simultaneously with urine sediment examination. The sediment inspection step is the same as the morphological inspection of a single microscope. And the urine dry chemical analysis step is to insert a urine test strip into a test strip chemical reaction plate (test strip reaction component), open a liquid discharge opening mechanism, push a liquid discharge plug or a liquid discharge air bag, squeeze a liquid sample in a test tube to flow into the test strip in the test strip reaction plate from a liquid discharge port for chemical reaction, and then put the test strip into a urine analyzer for scanning analysis.

Example seven

Stool test strips (occult blood, rotavirus, etc.) were examined simultaneously with stool conventional microscope cell morphology. The routine cell morphology examination procedure of stool is the same as that of the microscope alone. The stool test strip checking step includes inserting one test strip into the test strip chemical reaction board, opening the liquid draining opening mechanism, pushing the liquid draining plug or the liquid draining air bag, extruding the liquid sample inside the test tube to flow from the liquid draining port onto the test strip to perform chemical reaction, and finally judging by human eye or automatic judging by the instrument after taking picture with the camera.

Example eight

Gynecological leucorrhea secretion test strips (BV cell bacterial vaginitis test strips and the like) and leucorrhea conventional microscope morphology are checked simultaneously. The white-banded conventional microscopy morphological examination procedure is the same as the single microscopy morphological examination. And the step of checking the white test strip, namely inserting a test strip into the test strip chemical reaction plate, opening a liquid discharge opening mechanism, pushing a liquid discharge plug or a liquid discharge air bag, extruding a liquid sample in the test tube to flow into the test strip in the test strip reaction plate from a liquid discharge port for chemical reaction, and finally judging by human eyes or automatically judging by an instrument after a camera shoots pictures.

Example nine

Serum various test strips were examined simultaneously with the blood cell microscope morphology. The blood cell microscopy morphological examination procedure is the same as the microscopy morphological examination alone. The detection step of various test strips of serum comprises the steps of inserting a test strip into a test strip chemical reaction plate, opening a liquid discharge opening mechanism, pushing a liquid discharge plug or a liquid discharge air bag, extruding a liquid sample in a test tube to flow into the test strip in the test strip reaction plate from a liquid discharge port for chemical reaction, and finally judging by human eyes or automatically judging by an instrument.

Examples ten

The test strips in the above examples were changed to inspection of various standard test paper boards on the market (each manufacturer already installed the test strips in a colloidal board). The operation method comprises the steps of placing a standard test paper chemical reaction plate on the market below a test tube liquid discharge opening mechanism, aligning a liquid adding hole of the standard test paper reaction plate with a liquid outlet of the liquid discharge opening mechanism, opening the liquid discharge opening mechanism without installing the test paper chemical reaction plate on the side surface of the liquid discharge opening mechanism, pushing a liquid discharge plug or a liquid discharge air bag, extruding a liquid sample in the test tube to be dripped into the liquid adding hole of the standard test paper reaction plate below from the liquid outlet, and finally judging by human eyes or automatically judging by an instrument.

The 10 embodiments above can be operated either manually or automatically. Meanwhile, more than 1 test strip chemical reaction plate can be added on the side face of the test strip chemical reaction plate, the test strip chemical reaction plate can be arranged on the two side faces and the parallel bottom of the test tube body, the number of liquid discharge holes can be multiple, the cylindrical liquid discharge column can be changed into a plurality of equidistant thick-thin interphase structures to realize simultaneous opening and closing of the liquid discharge holes, the strip-shaped liquid discharge type can be changed into a plurality of equidistant liquid discharge holes with the liquid discharge opening mechanism, and the same number of equidistant liquid discharge holes are also added in the place where liquid discharge is inserted and opened in the test tube body.

The processor of the scheme integrates biological sample treatment (sampling, loading, dissolving and centrifuging), a cell counting pool (optical observation area), liquid drainage and a test strip chemical reaction plate, so that the operation steps are more concise and convenient, the time is saved, the cost is saved, the quality can be effectively improved, and the pollution is reduced. In the aspect of biological samples to be subjected to microscopic examination, a manual operator does not need to use a pipette to suck the biological samples from a test tube and then inject the biological samples into a glass slide, a counting plate and a test strip chemical reaction plate, and an automatic instrument does not need to adopt a sample sucking needle, a peristaltic pump, an electromagnetic valve and a complex silica gel pipeline to convey the biological samples to the glass slide, the counting plate and the test strip chemical reaction plate. The scheme avoids complex liquid layering and transferring (such as taking sediment or supernatant after centrifugation) operation of biological samples, liquid transferring is not needed after centrifugation, liquid is not needed to be removed firstly, then cell components such as sediment are taken, and liquid draining is controlled by a switch device due to detection and sample treatment in a container communicated with the container. Based on the characteristics, the integrated multifunctional biological sample processor of the scheme has outstanding substantive characteristics and remarkable progress compared with the existing similar scheme.

The integrated multifunctional biological sample processor of the present solution is not limited to the disclosure in the specific embodiments, and the technical solutions presented in the examples may be extended based on the understanding of the person skilled in the art, and simple alternatives made by the person skilled in the art according to the present solution in combination with common general knowledge also belong to the scope of the present solution.

Claims

1. An integrated multifunctional biological sample processor is characterized in that,

The biological sample processing device comprises a test tube body, wherein the test tube body comprises a sampling processing section and an optical observation section, the sampling processing section is communicated with the optical observation section to form a whole test tube body, a test tube plug is arranged on an upper cover of a test tube body inlet at one end of the sampling processing section, a biological sample entering the sampling processing section is processed to form a sampling liquid, the sampling liquid enters the optical observation section, and external inspection equipment observes a sample and acquires sample parameters through the optical observation section;

The test tube comprises a test tube body, and is characterized in that a liquid discharge tube body is arranged on the outer side of the test tube body, a liquid taking through hole communicated with an optical observation section of the test tube body and a liquid discharge through hole communicated with a test paper reaction assembly arranged on the outer side of the test tube body are arranged on the liquid discharge tube body, a liquid discharge guide post is arranged in the liquid discharge tube body, the liquid discharge guide post comprises a cut-off section and a through flow section, the outer diameter of the through flow section is smaller than that of the cut-off section, the cut-off section is in gapless sliding contact with the inner side wall of the liquid discharge tube body, a liquid discharge loop is formed by the through flow section and the inner side wall of the liquid discharge tube body, the liquid taking through hole, the liquid discharge through hole and the liquid discharge loop are communicated, and the test paper plug is pushed to discharge sampling liquid from the liquid taking through hole, the liquid discharge loop and the liquid discharge through hole into the test paper reaction assembly;

A sampling baffle A is arranged in the rear section of the sampling treatment section of the test tube body, a circulation gap is arranged between the sampling baffle A and the inner side wall of the sampling treatment section, the sampling baffle A comprises a slope-shaped diversion surface, a biological sample in the sampling treatment section is centrifugally treated and then deposited to the optical observation section through the slope-shaped diversion surface, and backflow is prevented by the sampling baffle A when the test tube body is horizontally placed, or,

A sampling baffle B is arranged in the rear section of the sampling treatment section of the test tube body, the sampling baffle B is tightly combined with the inner side wall of the sampling treatment section along the circumferential direction, a plurality of filtering through holes are arranged on the sampling baffle B, biological samples in the sampling treatment section are uniformly mixed by an oscillator and then enter the optical observation section after being filtered by the filtering through holes, or,

The rear section of the sampling treatment section of the test tube body is internally provided with a sampling combined baffle which is in close contact with the inner side wall of the sampling treatment section along the circumferential direction, the sampling combined baffle comprises a baffle a, a baffle b and a baffle c which are sequentially connected through an elastic part to form the whole sampling combined baffle, the baffle b is provided with a connecting rod connected with the test tube plug, the inner end of the connecting rod is movably sleeved in a fixed guide ring, the side edge of the far end of the baffle a is connected with the guide ring through the elastic part, the remote side of the baffle c is connected with the guide ring through an elastic part, the test tube plug is pushed to be linked, the connecting rod is pushed to push the baffle b to enable the sampling combined baffle to be deformed and folded and form a circulation gap with the inner side wall of the sampling treatment section, a biological sample in the sampling treatment section enters the optical observation section through the circulation gap and exits the test tube plug, and the sampling combined baffle is reset under the action of the elastic part.

2. The integrated multifunctional biological sample processor according to claim 1, wherein part of the side wall of the sampling processing section is a cylindrical surface structure, part of the side wall of the optical observation section is a planar structure, the cylindrical surface structure and the planar structure are connected through an inclined surface structure to form part of the side wall of the whole test tube body, and the external inspection equipment observes a sample and collects sample parameters through the planar structure.

3. The integrated multifunctional biological sample processor according to claim 1, wherein the test tube plug is a columnar structure with the shape matched with the inner side of the inlet of the test tube body, a plurality of circumferentially extending protruding sealing rings are arranged on the outer side of the columnar structure, the test tube plug is pushed to be tightly covered to discharge sampling liquid into the test paper reaction assembly, and the sealing rings prevent the biological sample in the test tube body from being infiltrated outwards.

4. The integrated multifunctional biological sample processor of claim 1, wherein an elastic hollow ball is arranged at the outer end of the test tube plug, and the elastic hollow ball is extruded to discharge sampling liquid into the test paper reaction component.

5. The integrated multifunctional biological sample processor of claim 1, wherein the test tube stopper comprises a test tube stopper cavity, the test tube stopper cavity is fixed in the test tube body inlet, and a liquid draining piston is arranged on the part of the test tube body inlet side of the test tube stopper cavity, and pushes the liquid draining piston to drain sampling liquid into the test paper reaction component.

6. The integrated multifunctional biological sample processor according to claim 1, wherein a columnar quantitative liquid discharge piston is arranged at the outer end of the test tube plug, a liquid discharge scale is arranged on the outer side of the columnar quantitative liquid discharge piston along the liquid discharge direction, and the columnar quantitative liquid discharge piston is pushed to discharge sampling liquid into the test paper reaction component according to the liquid discharge scale.

7. The integrated multifunctional biological sample processor of claim 1, wherein the test tube plug is arranged at one end in the test tube body, the sampling square spoon comprises a spoon head and a spoon handle, the spoon handle is of a cylindrical structure, and the sampling Fang Shao is sleeved on a connecting handle arranged at one end in the test tube body through the spoon handle.

8. The integrated multifunctional biological sample processor of claim 7, wherein the spoon head of the sampling Fang Shao is provided with a plurality of through hole structures.

9. The integrated multifunctional biological sample processor of claim 1, wherein the test tube plug is arranged at one end in the test tube body and is provided with a fiber test probe, the fiber test probe comprises a fiber test probe head and a fiber test probe rod, the fiber test probe rod is of a cylindrical structure, and the fiber test probe is sleeved on a connecting handle arranged at one end in the test tube body through the fiber test probe rod.

10. The integrated multifunctional biological sample processor of claim 1, wherein the optical observation section comprises a slide, a cover slip is arranged on the slide, a fixing frame is fixedly pressed on the cover slip, the cover slip is buckled on the slide by the fixing frame, and an external inspection device observes a sample on the slide and collects sample parameters through the cover slip.

11. The integrated multifunctional biological sample processor according to claim 1, wherein the optical observation section is of a closed structure, a sample carrying sheet is arranged at a position corresponding to the optical observation section, and an external inspection device observes a sample on the sample carrying sheet and collects sample parameters through the optical observation section.

12. The integrated multifunctional biological sample processor according to claim 1, wherein a reset spring is arranged between the liquid discharge guide post and the inner bottom of the liquid discharge pipe body, the liquid discharge guide post is driven to communicate the liquid taking through hole, the liquid discharge through hole and the liquid discharge loop to complete liquid discharge operation, and the liquid discharge guide post is reset under the action of the reset spring.

13. The integrated multifunctional biological sample processor according to claim 1, wherein a liquid discharge groove is arranged on the outer side of the test tube body, a liquid taking through hole is formed in the liquid discharge groove, a liquid discharge sliding block is arranged in the liquid discharge groove, a liquid discharge through hole is formed in the liquid discharge sliding block, the liquid discharge through hole is communicated with the liquid taking through hole, and the test tube plug is pushed to discharge sampling liquid from the liquid taking through hole and the liquid discharge through hole into a test paper reaction assembly fixed on the liquid discharge sliding block.

14. The integrated multifunctional biological sample processor according to claim 1 or 13, wherein the test paper reaction component comprises an elongated slot substrate, a test paper strip insertion plate is arranged in a slot of the elongated slot substrate, a test paper strip is arranged in the test paper strip insertion plate, and a sampling liquid discharged from the liquid discharge through hole and the test paper strip are subjected to detection chemical reaction.

15. The integrated multifunctional biological sample processor according to claim 14, wherein the long-strip-shaped groove substrate is of a cuboid structure, a long-side wide surface A on one side of the long-strip-shaped groove substrate is open, a short-side narrow surface A on one side adjacent to the long-side wide surface A is open, the test strip inserting plate is inserted into the long-strip-shaped groove substrate from the short-side narrow surface A, a plurality of round-head limiting block structures protruding into the long-side wide surface A are arranged on the opening edge of the long-side wide surface A, and the round-head limiting block structures prevent the test strip inserting plate from falling from the long-side wide surface A.

16. The integrated multifunctional biological sample processor according to claim 15, wherein a long side wide surface C on one side of the test strip plugboard is provided with an opening, a test strip is placed into the test strip plugboard from the long side wide surface C, a plurality of round head limiting block structures protruding towards the inside of the test strip plugboard are arranged on the inner side edge of the opening of the long side wide surface C, and the round head limiting block structures prevent the test strip from falling from the long side wide surface C.

17. The integrated multifunctional biological sample processor according to claim 11 or 13, wherein the test paper reaction component comprises a strip-shaped groove substrate, a test paper strip groove is arranged in a slot of the strip-shaped groove substrate, a reaction round through hole A and a reaction long through hole A are arranged at the bottom of the test paper strip groove, a test paper strip is arranged in the test paper strip groove, a test paper strip pressing plate is arranged above the test paper strip groove in the strip-shaped groove substrate, a reaction round through hole B and a reaction long through hole B are arranged on the test paper strip pressing plate, the reaction round through hole B corresponds to the reaction round through hole A, the reaction long through hole B corresponds to the reaction long through hole A, and sampling liquid discharged from the liquid discharge through hole enters the reaction round through hole B and the reaction long through hole B to carry out detection chemical reaction with the test paper strip.

18. The integrated multifunctional biological sample processor according to claim 1, wherein an L-shaped liquid discharge pipe is arranged on the outer side of the test tube body, one end of the L-shaped liquid discharge pipe is communicated with the optical observation section, a liquid discharge control cap is arranged on the other end of the L-shaped liquid discharge pipe, and the test tube plug is pushed to discharge sampling liquid from the other end of the L-shaped liquid discharge pipe into the test paper reaction assembly.

19. The integrated multifunctional biological sample processor according to claim 18, wherein the other end of the L-shaped liquid discharge tube has a tube structure with an outer diameter tapered along a liquid discharge direction, the liquid discharge control cap is pushed along a release direction to form a limit contact with a cap stopper provided on the outside of the test tube body, and the sampling liquid is discharged along a gap between the other end of the L-shaped liquid discharge tube and the inside of the liquid discharge control cap.

20. The integrated multifunctional biological sample processor according to claim 19, wherein the end head of the other end of the L-shaped liquid discharge tube is of a closed structure, a liquid discharge hole is formed in the outer side of the other end of the L-shaped liquid discharge tube, the liquid discharge hole is communicated with the optical observation section through an inner cavity of the L-shaped liquid discharge tube, the liquid discharge control cap is pushed in the disengaging direction to form limiting contact with a cap stop block arranged on the outer side of the test tube body, and sampling liquid is discharged from the liquid discharge hole.

21. The integrated multifunctional biological sample processor according to claim 19, wherein a spring is sleeved on the other end of the L-shaped liquid discharge tube, the spring presses the liquid discharge control cap against a cap stop block on the outer side of the test tube body to form limit contact, the end head of the other end of the L-shaped liquid discharge tube is of a closed structure, a liquid discharge hole is formed in the outer side of the other end of the L-shaped liquid discharge tube, the liquid discharge control cap is pushed to overcome the stress of the spring to open the liquid discharge hole, and sampling liquid is discharged from the liquid discharge hole.

22. The integrated multifunctional biological sample processor of claim 1, wherein a liquid discharge nozzle is arranged on the distal end of the optical observation section, a liquid discharge control cap is sleeved on the liquid discharge nozzle, and the test tube plug is pushed to discharge sampling liquid from the liquid discharge nozzle into the test paper reaction component.

23. An integrated multifunctional biological sample processor is characterized in that,

The test tube plug comprises a test tube plug cavity, the test tube plug cavity is fixed in the inlet of the test tube body, a liquid draining piston is arranged on the position of the inlet side of the test tube body in the test tube plug cavity, and the liquid draining piston is pushed to drain sampling liquid into the test paper reaction assembly;

The test tube is characterized in that a liquid discharge tube body is arranged on the outer side of the test tube body, a liquid taking through hole communicated with the optical observation section of the test tube body and a liquid discharge through hole communicated with the test paper reaction assembly arranged on the outer side of the liquid discharge tube body are arranged on the liquid discharge tube body, a liquid discharge guide pillar is arranged in the liquid discharge tube body, the liquid discharge guide pillar comprises a cut-off section and a through flow section, the outer diameter of the through flow section is smaller than that of the cut-off section, the cut-off section is in gapless sliding contact with the inner side wall of the liquid discharge tube body, the through flow section and the inner side wall of the liquid discharge tube body form a liquid discharge loop, the liquid taking through hole, the liquid discharge through hole and the liquid discharge loop are communicated with each other, and the test tube plug is pushed to discharge sampling liquid into the test paper reaction assembly.

24. An integrated multifunctional biological sample processor is characterized in that,

The test tube is characterized in that a liquid discharge groove is arranged on the outer side of the test tube body, a liquid taking through hole is formed in the liquid discharge groove, a liquid discharge sliding block is arranged in the liquid discharge groove, a liquid discharge through hole is formed in the liquid discharge sliding block and communicated with the liquid taking through hole, and the test tube plug is pushed to discharge sampling liquid from the liquid taking through hole and the liquid discharge through hole into a test paper reaction assembly fixed on the liquid discharge sliding block.

25. An integrated multifunctional biological sample processor is characterized in that,

The test tube plug comprises a test tube plug cavity, the test tube plug cavity is fixed in the inlet of the test tube body, a liquid draining piston is arranged on the position of the inlet side of the test tube body in the test tube plug cavity, and the liquid draining piston is pushed to drain sampling liquid into the optical observation section;

An L-shaped liquid discharge pipe is arranged on the outer side of the test tube body, one end of the L-shaped liquid discharge pipe is communicated with the optical observation section, a liquid discharge control cap is arranged on the other end of the L-shaped liquid discharge pipe, the liquid discharge control cap is pushed, and the test tube plug is pushed to discharge sampling liquid from the other end of the L-shaped liquid discharge pipe into the test paper reaction assembly.

26. An integrated multifunctional biological sample processor is characterized in that,

The remote end of the optical observation section is provided with a liquid discharge nozzle, the liquid discharge nozzle is sleeved with a liquid discharge control cap, the liquid discharge control cap is used for controlling the discharge of sampling liquid, and the test tube plug is pushed to discharge the sampling liquid from the liquid discharge nozzle into the test paper reaction assembly.