CN110292655A - A kind of injection fillers preparation of hydroxyl apatite and preparation method thereof - Google Patents
A kind of injection fillers preparation of hydroxyl apatite and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/06—Flowable or injectable implant compositions
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Abstract
The present invention relates to injection fillers preparations of a kind of hydroxyl apatite and preparation method thereof, belong to medical cosmetology and bio-medical technology field.The injection fillers preparation of hydroxyl apatite provided by the invention, it is comprised the following components in parts by weight: 20~80 parts of hydroxyapatite, 1~20 part of gel-type vehicle, 1~20 part of osmotic pressure regulator, 100 parts of buffer salt solution.Using the injection fillers preparation of the hydroxyl apatite of method provided by the invention preparation, the residence time of hydroxyapatite in vivo can be extended, it prevents hydroapatite particles from migrating in injection site, improves hydroxyapatite micro-sphere in the persistence of injection site, simultaneously, it is low to the irritation of tissue, it can reach most natural effect after injection, the tissue of injection site is soft, no scleroma, it is not easy to cause granuloma reaction, it can be with the flaw on Fast Filling and greasy skin.
Description
Technical field
The invention belongs to medical cosmetologies and bio-medical technology field, and in particular to a kind of injection of hydroxyl apatite is filled out
Fill preparation and preparation method thereof.
Background technique
Soft tissue defect is corrected by shaping and beauty technology and repairs the skin of aging shrinkage, keeps the perfection of facial shape
A kind of universal phenomenon is had become with the youth.In various shaping and beauty technologies, injection beautifying technique accounts for the largest percentage, and accounts for about whole
The 40% of a beauty market.Injection beautifying technique is to carry out local modification to human body by way of injection, reaches local form
The beautifying technique that exquisite, configuration is coordinated.The technology has the characteristics that zero restores, is quick and highly-safe, is that beauty needs
The first choice for the person of asking.
Injection beauty is one kind of non-operating shaping beauty, life that can be artificial synthesized by biomaterial using the method for injection
The good Material injection of object compatibility enters skin corium or subcutaneous, is reached by the different mechanism of action and reduces skinfold or moulding
A kind of shaping and beauty method.
Hydroxyapatite is the main inorganic composition for constituting organism bone, tooth, and it is hard to have rapidly become human body in recent years
One of alternative materials of tissue, bioengineered tissue bracket, reparation, shaping and the medicine sustained and controlled release in terms of from more
Carry out more important role, it is shown that good development prospect.Hydroxyapatite has high-caliber biocompatibility, and particle can
The generation of stimulation of endogenous collagen.
But hydroxyapatite itself is very crisp, is not easy to shape, is slightly soluble in water.Hydroxyapatite in vivo about 3 months i.e. quilt
It absorbs, and may migrate, lead to that expected cosmetic result cannot be reached.
Summary of the invention
The purpose of the present invention is on the basis of existing technology, providing a kind of injection fillers preparation of hydroxyl apatite,
The residence time of hydroxyapatite in vivo can be extended, prevent hydroapatite particles from migrating in injection site, with
When correcting soft tissue defect and repairing the skin of aging shrinkage, the facial soft tissue of corium deep layer and skin corium can be enhanced,
Reach expected cosmetic result.
It is a further object of the present invention to provide a kind of preparation methods of the injection fillers preparation of above-mentioned hydroxyl apatite.
Technical scheme is as follows:
A kind of injection fillers preparation of hydroxyl apatite, it is comprised the following components in parts by weight: hydroxyapatite 20~
80 parts, 1~20 part of gel-type vehicle, 1~20 part of osmotic pressure regulator, 100 parts of buffer salt solution.
In a preferred embodiment, the injection fillers preparation of hydroxyl apatite prepared by the present invention, it includes following heavy
The component of amount part: 45~65 parts of hydroxyapatite, 5~15 parts of gel-type vehicle, 5~15 parts of osmotic pressure regulator, buffer salt solution
100 parts.
In mixture of the present invention using sodium carboxymethylcellulose, hypromellose, carbomer and triethanolamine
One or more are used as gel-type vehicle, and the gel of available high elastic modulus and high dynamic viscosity extends hydroxyapatite and exists
It the intracorporal residence time, prevents hydroapatite particles from migrating in injection site, improves hydroxyapatite micro-sphere and injecting
The persistence at position.
In a preferred embodiment, gel-type vehicle can be the mixture of carbomer and triethanolamine.
It is further preferred that the mass ratio of carbomer and triethanolamine is 1:0.5~2.0, preferably 1:1.0~1.5.
The osmotic pressure regulator that the present invention refers to is one or more of glycerol, mannitol, propylene glycol or sorbierite；It is excellent
It is selected as glycerol or mannitol.
It is 0.9% sodium chloride solution or phosphate buffer solution as buffer salt solution that the present invention, which uses mass concentration,.
Wherein, phosphate buffer solution is the mixed solution of sodium dihydrogen phosphate, disodium phosphate soln or both.Preferably, originally
Invent the phosphate buffer solution that the phosphate buffer solution used is 5.5~7.5 for pH.For example, the phosphorus that pH is 5.5~7.5
The sodium dihydrogen phosphate and phosphoric acid that the disodium phosphate soln or pH that acid dihydride sodium solution, pH are 5.5~7.5 are 5.5~7.5
The mixed solution of disodium hydrogen solution.
The present invention also provides the preparation method of the injection fillers preparation of above-mentioned hydroxyl apatite, it the following steps are included:
Hydroxyapatite is ground, after 10~70 μm of microballoons are made, then with gel-type vehicle, osmotic pressure regulator and buffer salt solution
Be uniformly mixed to get.
In a preferred embodiment, the preparation method of the injection fillers preparation of above-mentioned hydroxyl apatite, it includes following
In further detail the step of: hydroxyapatite is ground, after 10~70 μm of microballoons are made, then with gel-type vehicle, osmotic pressure tune
Agent and buffer salt solution are saved, is stirred evenly under condition of negative pressure, transparent or semitransparent viscous gum, then filling, sterilizing is made.
The condition of negative pressure that the present invention refers to, refer to be stirred under conditions of partial vacuum uniformly, such as-
0.02MPa~-0.1MPa bubble can occurs to avoid when stirring, extend the reaction time.
Hydroxyapatite is first ground, is made in the injection fillers preparation for preparing hydroxyl apatite by the present invention
10~70 μm of microballoons grind hydroxyapatite in the case where not influencing effect of the present invention, be made 20~60 μm it is micro-
Ball；It is preferably made from 25~45 μm of microballoons.When hydroxyapatite will be made to 10~70 μm of microballoons, in the cooperation of other conditions
Under, the injection fillers preparation of the hydroxyl apatite of preparation is low to the irritation of tissue, can reach most natural effect after injection
The tissue of fruit, injection site is soft, no scleroma, granuloma reaction is not easy to cause, in addition, ensuring that while facilitating injection
Microballoon is not swallowed by human monocyte, macrophage, is taken away, and can enduringly stay in therapentic part, reaches expected beauty
Effect.
The injection fillers preparation of hydroxyl apatite prepared by the present invention, after being injected into tissue, the injection fillers system
Gel-type vehicle in agent is gradually degraded and absorbed after 1~3 month, no longer plays a supportive role；And hydroxyapatite micro-sphere is timely
Ground stimulation generates fibrous connective tissue or collagen, continues to play a supporting role.
The injection fillers preparation of hydroxyl apatite prepared by the present invention, can be with the flaw on Fast Filling and greasy skin
Defect, such as wrinkle, striae of pregnancy, promote new dermal cell growth, promote facial state rejuvenation, and have anti-senescence function.
The gel-type vehicle that the present invention uses is sodium carboxymethylcellulose, hypromellose or carbomer and triethanolamine
One or more of mixture.
In a preferred embodiment, gel-type vehicle can be the mixture of carbomer and triethanolamine.
It is further preferred that the mass ratio of carbomer and triethanolamine is 1:0.5~2.0, preferably 1:1.0~1.5.
In a kind of scheme, the above-mentioned osmotic pressure regulator referred to is one in glycerol, mannitol, propylene glycol or sorbierite
Kind is several；Preferably glycerol or mannitol.
It is 0.9% sodium chloride solution or phosphate buffer solution as buffer salt solution that the present invention, which uses mass concentration,.
Wherein, phosphate buffer solution can be the mixed solution of sodium dihydrogen phosphate, disodium phosphate soln or both.For example,
The phosphoric acid that the disodium phosphate soln or pH that sodium dihydrogen phosphate that pH is 5.5~7.5, pH are 5.5~7.5 are 5.5~7.5
The mixed solution of dihydro sodium solution and disodium phosphate soln.
Using technical solution of the present invention, advantage is as follows:
The injection fillers preparation of hydroxyl apatite prepared by the present invention, after being injected into tissue, the injection fillers system
Gel-type vehicle in agent is gradually degraded and absorbed after 1~3 month, no longer plays a supportive role；And hydroxyapatite micro-sphere is timely
Ground stimulation generates fibrous connective tissue or collagen, continues to play a supporting role.
The injection fillers preparation of hydroxyl apatite provided by the invention can extend the delay of hydroxyapatite in vivo
Time prevents hydroapatite particles from migrating in injection site, improves hydroxyapatite micro-sphere in the lasting of injection site
Property.It is low to the irritation of tissue when for correcting soft tissue defect and repairing the skin of aging shrinkage, it can reach most after injection
The tissue of natural effect, injection site is soft, no scleroma, is not easy to cause granuloma reaction, with Fast Filling and can lubricate
Flaw on skin, such as wrinkle, striae of pregnancy, promote new dermal cell growth, promote facial state rejuvenation, and have anti-
Aging function.
Specific embodiment
The following description is intended to explain the principle of the present invention and main feature, not to the raw material group in the present invention
There is specific restriction effect at, ratio.
Embodiment 1
55 parts of hydroxyapatite are weighed, the microballoon at 25~45 μm is smashed and ground；To ground hydroxyapatite micro-sphere
Middle 3 parts of addition sodium carboxymethylcellulose, 3.9 parts of mannitol, continue grinding uniformly, and the mixed material after grinding is added 100 parts
In the phosphate buffer solution that pH is 6.5, stirred 3 hours under the conditions of negative pressure (- 0.02Mpa), blowing, it is filling to 2.25ml inoculated
It seals in syringe, gamma-ray irradiation sterilizing.
Embodiment 2
40 parts of hydroxyapatite are weighed, the microballoon at 10~20 μm is smashed and ground；To ground hydroxyapatite micro-sphere
Middle 1 part of addition carbomer, 2 parts of triethanolamine, 5 parts of glycerol, continue grinding uniformly, and the mixed material after grinding is added 100 parts
In the phosphate buffer solution that pH is 5.5, stirred 5 hours under the conditions of negative pressure (- 0.05Mpa), blowing, it is filling to 2.25ml inoculated
It seals in syringe, gamma-ray irradiation sterilizing.
Embodiment 3
65 parts of hydroxyapatite are weighed, the microballoon at 50~70 μm is smashed and ground；To ground hydroxyapatite micro-sphere
Middle 5 parts of addition hypromellose, 2 parts of mannitol, continue grinding uniformly, and the mixed material after grinding is added 100 parts
In 0.9% sodium chloride solution, stirred 5 hours under the conditions of negative pressure (- 0.07MPa), blowing, it is filling to 2.25ml pre-encapsulated injector
In, gamma-ray irradiation sterilizing.
Embodiment 4
80 parts of hydroxyapatite are weighed, the microballoon at 25~45 μm is smashed and ground；To ground hydroxyapatite micro-sphere
Middle 1.5 parts of addition carbomer, 3.5 parts of triethanolamine, 3 parts of mannitol continue grinding uniformly, the mixed material after grinding are added
In the phosphate buffer solution that 100 parts of pH are 6.5, stirred 8 hours under the conditions of negative pressure (- 0.04MPa), blowing, it is filling extremely
In 2.25ml pre-encapsulated injector, gamma-ray irradiation sterilizing.
Embodiment 5
30 parts of hydroxyapatite are weighed, the microballoon at 25~45 μm is smashed and ground；To ground hydroxyapatite micro-sphere
Middle 2.5 parts of addition sodium carboxymethylcellulose, 2 parts of glycerol, continue grinding uniformly, and 100 parts of pH are added in the mixed material after grinding
It is filling to the pre- encapsulating of 2.25ml to be stirred 3 hours under the conditions of negative pressure (- 0.06MPa), blowing in 7.5 phosphate buffer solution
In syringe, gamma-ray irradiation sterilizing.
Comparative example 1
50 parts of hydroxyapatite are weighed, the microballoon at 2~5 μm is smashed and ground；Into ground hydroxyapatite micro-sphere
1.5 parts of carbomer, 3.5 parts of triethanolamine, 3 parts of mannitol is added, continues grinding uniformly, the mixed material after grinding is added
It in the phosphate buffer solution of 100 parts of pH6.5, is stirred 8 hours under the conditions of negative pressure (- 0.05MPa), blowing is filling to 2.25ml
In pre-encapsulated injector, gamma-ray irradiation sterilizing.
Comparative example 2
40 parts of hydroxyapatite are weighed, the microballoon at 80~100 μm is smashed and ground；To ground hydroxyapatite micro-sphere
Middle 2.5 parts of addition sodium carboxymethylcellulose, 2 parts of glycerol, continue grinding uniformly, and the mixed material after grinding is added 100 parts
In the phosphate buffer solution of pH7.5, stirred 3 hours under the conditions of negative pressure (- 0.04MPa), blowing, it is filling to the pre- encapsulating of 2.25ml
In syringe, gamma-ray irradiation sterilizing.
Injection fillers preparation obtained by above-described embodiment is detected, the results are shown in Table 1.
1 sample measurement result of table
Stability test:
Influence factor hot test: sample made from Examples 1 to 5 being set in 60 DEG C of baking ovens and is placed 10 days, observes hydroxyl
Apatite settles situation.
The test of influence factor strong illumination: sample made from Examples 1 to 5 being set in the lighting box equipped with fluorescent lamp,
Illumination is placed 10 days under conditions of being 4500lx scholar 500lx, and observation hydroxyapatite settles situation.
Accelerated test: by sample made from Examples 1 to 5, in 40 DEG C of temperature, 2 DEG C of scholar, the item of relative humidity 75% ± 5%
It is placed 6 months under part, observation hydroxyapatite settles situation.
Long term test: by sample made from Examples 1 to 5, at 25 DEG C ± 2 DEG C of temperature, relative humidity 60% ± 10%
Under the conditions of place 12 months, observation hydroxyapatite settle situation.
Stability test the results show that the product for preparing of the embodiment of the present invention 1~5 and comparative example 1 test in influence factor,
It is placed under the conditions of accelerated test, long term test etc., hydroapatite particles are dispersed in gelinite always, do not there is deposition feelings
Condition.And comparative example 2 accelerate 6 months and it is long-term place 12 months after, particle has sedimentation phenomenon, illustrate hydroxyapatite partial size with
Sedimentation has certain relationship.
It is subcutaneously implanted effect:
Sample prepared by the sample and comparative example 1 for taking the 0.5ml embodiment of the present invention 1~5 to prepare, is implanted into rat skin respectively
Under, observation.
After Examples 1 to 5 sample injection, the reaction such as red, swollen, livid purple is not found.
It is subcutaneously implanted 1 month, sample implant site filling effect prepared by comparative example 1 is unobvious, hydroapatite particles
It migrates, illustrates that the partial size of hydroxyapatite is too small, easily migrate, or swallowed by internal macrophage, do not have and answer
It is effective.The sample filling effect of Examples 1 to 5 preparation is obvious, surrounding tissue variation without exception.
It is subcutaneously implanted 6 months, Examples 1 to 5 is fully wrapped around by fibrous connective tissue, and filling effect is obvious, surrounding tissue
Variation without exception.
In order to verify the effect (be used as dermal filler) of product of the present invention, following trial test is carried out:
Subject:
40,30~60 one full year of life of age, health, face has obvious decree line, crow's feet, glabella line, eye rill, volume
Head line, lower eyelid recess and other Facial Depression persons etc., male or female.
Test method:
It takes the embodiment of the present invention 1 to prepare resulting product, is injected with 25G needle or 27G syringe needle, injection depth and dosage
Depending on position and degree.By needle in about 30 ° of angle insertion corium deep layer, inclined-plane downward, to reduce to the maximum extent
Implantation material deposits to more superficial face.By applying mild, continuous pressure on plunger rod come injected gel, while slowly taking out
Syringe needle out, thus organizing the formation of single uniform injected gel line.When correcting deeper wrinkle, should be carried out below wrinkle
It is placed in parallel.After the completion of injection, gently massage therapy region, it is ensured that gel is evenly distributed, and gel is moulded into tissue contours.?
After treatment in 24 hours, subject should carry out cold compress using ice bag to treatment region, to reduce rubescent, swelling and stimulation.
Conclusion:
Injection is observed after 48 hours, and in 40 people, 39 people injection sites are without redness, ecchymosis etc., no allergic reaction.1 people injection
There is erythema in position, and swelling is subsided after massage, ice bag cold compress.
Injection is observed after 3 months, is had 32 people's wrinkles or recess to completely disappear in 40 people, is reached and fill and lead up effect, accounting 80%；
There are 5 people's wrinkles or recess to improve obvious, reaches satisfied and fill and lead up effect, but wrinkle or recess naked eyes are still seen, accounting 12.5%；
There are 2 people's wrinkles or recess to have a degree of improvement, but improves result and be unsatisfied with, wrinkle or more obvious, the accounting 5% that is recessed；
There are 1 people's wrinkle or recess preceding without significant change, wrinkle or high-visible, the accounting 2.5% that is recessed with injection.
Verified, product of the present invention is safe to the human body effectively, and total effective rate is up to 97.5%.
The experimental data of above-described embodiment has absolutely proved the injection fillers system of hydroxyl apatite prepared by the present invention
Agent, form stable homogeneous, physicochemical properties meet clinical requirement, are suitable for beauty and fill plastotype.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that: it still may be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features；And these modification or
Replacement, the range for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (10)
1. a kind of injection fillers preparation of hydroxyl apatite, which is characterized in that it is comprised the following components in parts by weight: hydroxyl phosphorus
20~80 parts of lime stone, 1~20 part of gel-type vehicle, 1~20 part of osmotic pressure regulator, 100 parts of buffer salt solution.
2. the injection fillers preparation of hydroxyl apatite according to claim 1, which is characterized in that it includes following weight
The component of part: 45~65 parts of hydroxyapatite, 5~15 parts of gel-type vehicle, 5~15 parts of osmotic pressure regulator, buffer salt solution 100
Part.
3. the injection fillers preparation of hydroxyl apatite according to claim 1, which is characterized in that the gel-type vehicle is
One or more of sodium carboxymethylcellulose, hypromellose or carbomer and the mixture of triethanolamine.
4. the injection fillers preparation of hydroxyl apatite according to claim 3, which is characterized in that the gel-type vehicle is
The mixture of carbomer and triethanolamine, it is preferable that the mass ratio of carbomer and triethanolamine be 1:0.5~2.0, preferably 1:
1.0~1.5.
5. the injection fillers preparation of hydroxyl apatite according to claim 1, which is characterized in that the osmotic pressure is adjusted
Agent is one or more of glycerol, mannitol, propylene glycol or sorbierite；Preferably glycerol or mannitol.
6. the injection fillers preparation of hydroxyl apatite according to claim 1, which is characterized in that the buffer salt solution
For mass concentration be 0.9% sodium chloride solution or phosphate buffer solution；The phosphate buffer solution is sodium dihydrogen phosphate
The mixed solution of solution, disodium phosphate soln or both.
7. the injection fillers preparation of hydroxyl apatite according to claim 6, which is characterized in that the phosphate-buffered
Solution, selected from pH be 5.5~7.5 sodium dihydrogen phosphate, pH be 5.5~7.5 disodium phosphate soln or pH be 5.5~
7.5 sodium dihydrogen phosphate and the mixed solution of disodium phosphate soln.
8. a kind of preparation method of the injection fillers preparation of hydroxyl apatite, which is characterized in that it is the following steps are included: by hydroxyl
Base apatite is ground, and after 10~70 μm of microballoons are made, then is mixed with gel-type vehicle, osmotic pressure regulator and buffer salt solution
Uniformly to get.
9. the preparation method of the injection fillers preparation of hydroxyl apatite according to claim 8, which is characterized in that by hydroxyl
Base apatite is ground, and 20~60 μm of microballoons are made；It is preferably made from 25~45 μm of microballoons.
10. the preparation method of the injection fillers preparation of hydroxyl apatite according to claim 8, which is characterized in that institute
State one of mixture that gel-type vehicle is sodium carboxymethylcellulose, hypromellose or carbomer and triethanolamine or
It is several；The osmotic pressure regulator is one or more of glycerol, mannitol, propylene glycol or sorbierite；The buffer salt is molten
Liquid is the sodium chloride solution or phosphate buffer solution that mass concentration is 0.9%；The phosphate buffer solution is biphosphate
The mixed solution of sodium solution, disodium phosphate soln or both.
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Cited By (6)
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| CN110787319A (en) * | 2019-11-19 | 2020-02-14 | 上海摩漾生物科技有限公司 | Implant for facial cosmetic lifting and application thereof |
| CN114146219A (en) * | 2021-12-29 | 2022-03-08 | 上海璞聚生物科技有限公司 | Soft tissue filler and preparation method thereof |
| CN115137877A (en) * | 2022-03-25 | 2022-10-04 | 杭州诺嫣生物科技有限公司 | Injectable gel composition, preparation method and application thereof |
| CN116570765A (en) * | 2023-06-02 | 2023-08-11 | 河北优固生物科技有限公司 | Human soft tissue filling material and preparation method thereof |
| CN116832209A (en) * | 2022-03-25 | 2023-10-03 | 杭州诺嫣生物科技有限公司 | Soft tissue filler composition, preparation method and application thereof, and facial injection preparation |
| CN118924948A (en) * | 2024-05-31 | 2024-11-12 | 复向丝泰医疗科技(苏州)有限公司 | Silk protein composite hydroxyapatite microsphere filler for injection and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63317159A (en) * | 1987-06-22 | 1988-12-26 | Sangi:Kk | Bone filling material |
| CN1826147A (en) * | 2003-05-22 | 2006-08-30 | 阿托斯有限责任公司 | Inorganic resorbable bone substitute material |
| CN108744054A (en) * | 2018-06-15 | 2018-11-06 | 北京水元生生物科技有限公司 | A kind of injection-type beauty and shaping facial bulking agent compositions gel and preparation method thereof |
-
2019
- 2019-07-18 CN CN201910650833.5A patent/CN110292655A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63317159A (en) * | 1987-06-22 | 1988-12-26 | Sangi:Kk | Bone filling material |
| CN1826147A (en) * | 2003-05-22 | 2006-08-30 | 阿托斯有限责任公司 | Inorganic resorbable bone substitute material |
| CN108744054A (en) * | 2018-06-15 | 2018-11-06 | 北京水元生生物科技有限公司 | A kind of injection-type beauty and shaping facial bulking agent compositions gel and preparation method thereof |
Non-Patent Citations (7)
| Title |
|---|
| D.R. PEREIRA AT EL.: "《Injectable gellan-gum/hydroxyapatite-based bilayered hydrogel composites for osteochondral tissue regeneration》", 《APPLIED MATERIALS TODAY》 * |
| 傅超美等: "《药用辅料学》", 31 October 2008, 中国中医药出版社 * |
| 孟胜男等: "《药剂学实验指导》", 29 February 2016, 中国医药科技出版社 * |
| 潘求真等: "《细胞工程》", 31 July 2009, 哈尔滨工程大学出版社 * |
| 盛华刚等: "《中药药剂学课堂笔记》", 31 October 2018, 同济大学出版社 * |
| 莫锡荣等译: "《合成材料工业经济》", 21 August 1963, 中国工业出版社出版 * |
| 陈大明等: "《先进陶瓷材料的注凝技术与应用》", 30 November 2011, 国防工业出版社 * |
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| CN110787319A (en) * | 2019-11-19 | 2020-02-14 | 上海摩漾生物科技有限公司 | Implant for facial cosmetic lifting and application thereof |
| CN114146219A (en) * | 2021-12-29 | 2022-03-08 | 上海璞聚生物科技有限公司 | Soft tissue filler and preparation method thereof |
| CN115137877A (en) * | 2022-03-25 | 2022-10-04 | 杭州诺嫣生物科技有限公司 | Injectable gel composition, preparation method and application thereof |
| CN116832209A (en) * | 2022-03-25 | 2023-10-03 | 杭州诺嫣生物科技有限公司 | Soft tissue filler composition, preparation method and application thereof, and facial injection preparation |
| CN116570765A (en) * | 2023-06-02 | 2023-08-11 | 河北优固生物科技有限公司 | Human soft tissue filling material and preparation method thereof |
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