CN110161007A - A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence - Google Patents

Abstract

A kind of optical detection apparatus for the synchronous detection of multi-wavelength fluorescence, including detection pedestal and the detection module for being placed with reagent, it is provided with excitation circle in the detection pedestal and receives circle, the excitation circle is connect by excitation fiber with detection module, and the reception circle is connect by reception optical fiber with detection module；Stepper motor is installed in the detection pedestal, the detection turntable that rotation is driven by it is installed on output shaft of stepper motor, the one-to-one detection components of the multiple groups rotated with it and light source assembly are installed on the detection turntable, the light source assembly is located at the underface of excitation circle, and the detection components are located at the underface for receiving circle.Detection turntable of the invention, which rotates a circle, can be realized multiple Channel Synchronous detection of different reagents, greatly improve detection efficiency.

Description

An optical detection device for simultaneous detection of multi-wavelength fluorescence

technical field

The invention belongs to the technical field of biology and medical detection, and relates to an optical detection device for synchronous detection of multi-wavelength fluorescence.

Background technique

Real-time fluorescence quantitative PCR technology can realize the quantitative analysis of DNA templates, which is of great significance to molecular biology research and medical research. At present, most quantitative PCR instruments basically use photography or optical fiber to detect reagents. The light source generally uses halogen lamps, which will attenuate after long-term use, resulting in inaccurate measurement results, and photographs have edge effects. It will lead to inaccurate measurement results, while the other uses optical fiber and cooperates with PMT photomultiplier tubes for detection. Because PMT photomultiplier tubes are relatively expensive and relatively large in size, it can only be used with linear guides or screw rods for reagent detection. Carry out detection by scanning one by one. If the reagent is a multi-channel reagent, it is necessary to wait for one channel to be detected before switching to the next channel for detection when changing the channel during detection. In this way, the detection rate is relatively slow. If the reagent has requirements for the detection rate, this detection method does not apply.

Contents of the invention

Aiming at the current situation, the present invention provides an optical detection device for synchronous detection of multi-wavelength fluorescence. It first leads the required detection reagents to a detection disk through an optical fiber, and uses PD and slip rings to perform simultaneous detection. , greatly improving the detection efficiency.

The technical scheme adopted in the present invention is:

An optical detection device for synchronous detection of multi-wavelength fluorescence, comprising a detection base and a detection module placed with reagents, characterized in that: the detection base is provided with an excitation circle and a reception circle, and the excitation circle passes through the excitation The optical fiber is connected to the detection module, and the receiving ring is connected to the detection module through the receiving optical fiber; a stepper motor is installed on the detection base, and a detection turntable driven by it to rotate is installed on the output shaft of the stepper motor. A plurality of sets of one-to-one corresponding detection components and light source components are installed on the detection turntable as it rotates, the light source components are located directly below the excitation circle, and the detection components are located directly below the receiving circle. The detection turntable of the present invention can realize synchronous detection of multiple channels of different reagents by one rotation, greatly improving the detection efficiency.

Further, the light source control line of the light source assembly and the signal line of the detection assembly are connected to the external control board through the slip ring on the detection turntable, which can avoid the wires being entangled when rotating.

Further, 6 sets of detection components and light source components are evenly distributed on the detection turntable, and each set of detection components and light source components are staggered by 30°.

Further, the exciting ring is arranged inside the receiving ring, and both the exciting ring and the receiving ring are provided with 96 holes.

Further, 96 test tube holes for placing reagents are arranged in the detection module.

Further, the detection assembly includes a detection sleeve, in which a first convex lens, a receiving filter, an isolation column, and a PD detection board are sequentially installed, and the end cover of the detection sleeve is provided with a PD detection board. The cover, the first convex lens is set facing the detection ring.

Further, the light source assembly includes a light source cover, in which a second convex lens, a convex lens seat, an excitation filter, a plastic column, and an LED light board are installed in sequence, and the LED light board is arranged at the end of the light source cover. The second convex lens is set facing the excitation circle.

Further, the origin detection Hall plate is installed on the detection base, the origin magnetic steel base is installed on the detection turntable, and the magnetic steel for inductive origin detection Hall plate operation is installed in the origin magnetic steel base. The stepping motor of the present invention operates one cycle through the magnetic steel induction origin Hall plate in the origin magnetic steel base running to the origin position to ensure the reliability and accuracy of detection.

The present invention has the following outstanding advantages and beneficial effects: multiple sets of detection components and light source components with different channels are provided, and multiple channels of different reagents can be synchronously detected by one rotation, greatly improving the detection efficiency.

Description of drawings

Fig. 1 is a structural schematic diagram of the present invention.

Fig. 2 is a schematic top view of a partial structure of the present invention.

Fig. 3 is a bottom perspective schematic view of part of the structure of the present invention.

Fig. 4 is a schematic top view of the present invention after the detection base is removed.

Fig. 5 is a schematic diagram of the exploded structure of the detection assembly of the present invention.

Fig. 6 is a schematic diagram of the exploded structure of the light source assembly of the present invention.

In the figure: 1-stepper motor, 2-detection base, 3-detection component, 4-light source component, 5-slip ring, 6-detection turntable, 7-excitation fiber, 8-reception fiber, 9-origin magnet Seat, 10-Hall plate for origin detection, 11-Detection cover, 12-First convex lens, 13-Receiving filter, 14-Isolation column, 15-PD detection board, 16-PD cover, 17-Light source cover, 18 -convex lens seat, 19-excitation filter, 20-plastic column, 21-LED light board, 22-module, 23-second convex lens, 24-receiving circle, 25-exciting circle

Detailed ways

The present invention will be further described below in conjunction with specific examples, but the present invention is not limited to these specific implementations. Those skilled in the art will realize that the present invention covers all alternatives, modifications and equivalents as may be included within the scope of the claims.

In describing the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", " The orientations or positional relationships indicated by "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "clockwise", "counterclockwise" are based on the attached The orientation or positional relationship shown in the figure is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a reference to this invention. Invention Limitations. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly specifying the quantity of indicated technical features. Thus, a feature defined as "first" and "second" may explicitly or implicitly include one or more of these features. In the description of the present invention, unless otherwise specified, "plurality" means two or more, unless otherwise clearly defined.

In the present invention, unless otherwise clearly specified and limited, terms such as "installation", "connection", "connection" and "fixation" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection , or integrally connected; it may be mechanically connected or electrically connected; it may be directly connected or indirectly connected through an intermediary, and it may be the internal communication of two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention according to specific situations.

In the present invention, unless otherwise clearly specified and limited, a first feature being "on" or "under" a second feature may include direct contact between the first and second features, and may also include the first and second features Not in direct contact but through another characteristic contact between them. Moreover, "above", "above" and "above" the first feature on the second feature include that the first feature is directly above and obliquely above the second feature, or simply means that the first feature is horizontally higher than the second feature. "Below", "beneath" and "under" the first feature to the second feature include that the first feature is directly below and obliquely below the second feature, or simply means that the first feature has a lower level than the second feature.

Referring to Figures 1-4, this embodiment provides an optical detection device for simultaneous detection of multi-wavelength fluorescence, including a detection base 2 and a detection module 22 on which reagents are placed, and the detection base 2 is provided with an excitation ring 25 and a receiving ring 24, the exciting ring 25 is arranged on the inner side of the receiving ring 24, the exciting ring 25 and the receiving ring 24 are both provided with 96 holes, correspondingly, the detection module 22 is provided with 96 test tube holes for placing reagents, the excitation ring 25 is connected to the detection module 22 through the excitation optical fiber 8 , and the receiving ring 24 is connected to the detection module 22 through the receiving optical fiber 7 .

A stepper motor 1 is installed on the detection base 2 of this embodiment, and a detection turntable 6 driven by it to rotate is installed on the output shaft of the stepper motor 1. The six groups of rotating one-to-one corresponding detection components 3 and light source components 4, the detection components 3 are equipped with different receiving filters, and the light source components 4 are equipped with different excitation filters, thereby forming different detection channels and excitation channels, The detection assembly 3 and the light source assembly 4 of each group are arranged staggered by 30°; the light source assembly 4 is located directly below the excitation ring 25 and corresponds to the corresponding fiber hole, and the detection assembly 3 is located directly below the receiving ring 24 and corresponds to the corresponding fiber hole. Corresponding; the light source control line of the light source assembly 4 and the signal line of the detection assembly 3 are connected to the external control board through the slip ring 5 on the detection turntable 6, and the control board performs data processing, which can avoid winding the wire when rotating Together. The origin detection Hall plate 10 is installed on the detection base 2, the origin magnetic steel seat 9 is installed on the detection turntable 6, and the magnet for inductive origin detection Hall plate 10 operation is installed in the origin magnetic steel seat 9. steel. The stepping motor of the present invention operates one cycle through the magnetic steel induction origin Hall plate in the origin magnetic steel base running to the origin position to ensure the reliability and accuracy of detection. The detection turntable of the present invention can realize synchronous detection of multiple channels of different reagents by one rotation, greatly improving the detection efficiency.

The detection assembly 3 described in this embodiment includes a detection sleeve 11, in which a first convex lens 12, a receiving filter 13, an isolation column 14, and a PD detection plate 15 are sequentially installed in the detection sleeve 11, and the detection sleeve 11 is provided with a PD The end cover of the detection board 15 is provided with a PD cover 16 , and the first convex lens 12 is disposed facing the detection ring 24 , see FIG. 5 . The light source assembly 4 includes a light source cover 17, in which a second convex lens 23, a convex lens seat 18, an excitation filter 19, a plastic column 20, and an LED lamp board 21 are sequentially installed, and the LED lamp board 21 is provided with At the end of the light source cover 17, the second convex lens 23 is arranged facing the excitation ring 25, see FIG. 6 .

The detection components 3 of different channels and the light source components 4 of different channels of the present invention are fixed on the detection turntable 6, and the control lines of the light source group 3 and the signal lines of the detection component 4 are welded on the transfer circuit board, and fixed on the detection The wires on the slip ring 5 on the turntable 6 are led out to the control board for data processing, and the wires on the slip ring 5 are connected to circuit boards by amateurs. One end of multiple excitation optical fibers and receiving optical fibers is fixed on the detection base 2, and the other end It is fixed on the detection module 22, the reagent is put into the detection module 22, the stepper motor 1 is fixed on the detection base 2, and the detection turntable 6 is fixed on the output shaft of the stepper motor 1 by screws. 1 Rotate one circle, 6 sets of detection components 3 and light source components 4 detect at the same time, just complete the detection of 96 wells after running one circle, and realize simultaneous detection of multiple channels for different reagents, which greatly improves the detection efficiency.

Claims (8)

1. An optical detection device for synchronous detection of multi-wavelength fluorescence, comprising a detection base and a detection module placed with reagents, characterized in that: the detection base is provided with an excitation ring and a receiving ring, and the excitation ring The receiving ring is connected to the detection module through the excitation optical fiber, and the receiving ring is connected to the detection module through the receiving optical fiber; a stepping motor is installed on the detection base, and a detection turntable driven by it is installed on the output shaft of the stepping motor , the detection turntable is installed with multiple sets of one-to-one corresponding detection components and light source components that rotate with it, the light source components are located directly below the excitation circle, and the detection components are located directly below the receiving circle. 2. An optical detection device for synchronous detection of multi-wavelength fluorescence according to claim 1, characterized in that: the light source control line of the light source assembly and the signal line of the detection assembly pass through a slip ring on the detection turntable Connect with external control board. 3. An optical detection device for multi-wavelength fluorescence synchronous detection according to claim 1, characterized in that: 6 groups of detection components and light source components are evenly distributed on the detection turntable, and each group of detection components Set staggered 30° with the light source components. 4. A kind of optical detection device for synchronous detection of multi-wavelength fluorescence according to claim 1, characterized in that: the excitation circle is arranged on the inner side of the reception circle, and the excitation circle and the reception circle are both Set has 96 wells. 5. An optical detection device for synchronous detection of multi-wavelength fluorescence according to claim 4, characterized in that 96 test tube holes for placing reagents are arranged in the detection module. 6. An optical detection device for synchronous detection of multi-wavelength fluorescence according to any one of claims 1 to 5, characterized in that: the detection assembly includes a detection sleeve, and the detection sleeve is sequentially installed with first A convex lens, a receiving filter, an isolation column, and a PD detection plate, the detection sleeve is provided with a PD cover on the end cover of the PD detection plate, and the first convex lens is disposed facing the detection circle. 7. An optical detection device for synchronous detection of multi-wavelength fluorescence according to any one of claims 1 to 5, characterized in that: the light source assembly includes a light source cover, and the second light source cover is sequentially installed in the light source cover A convex lens, a convex lens seat, an excitation filter, a plastic column, and an LED lamp board, the LED lamp board is arranged at the end of the light source sleeve, and the second convex lens is arranged facing the excitation circle. 8. An optical detection device for synchronous detection of multi-wavelength fluorescence according to any one of claims 1 to 5, characterized in that: a Hall plate for origin detection is installed on the detection base, and the detection turntable An origin magnetic steel seat is installed on the top, and a magnetic steel for inductive origin detection Hall plate operation is installed in the origin magnetic steel seat.