CN110004106A - A kind of many cells magnetic separating method and system - Google Patents
A kind of many cells magnetic separating method and system Download PDFInfo
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- CN110004106A CN110004106A CN201910295169.7A CN201910295169A CN110004106A CN 110004106 A CN110004106 A CN 110004106A CN 201910295169 A CN201910295169 A CN 201910295169A CN 110004106 A CN110004106 A CN 110004106A
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 239000011324 bead Substances 0.000 claims abstract description 102
- 238000000926 separation method Methods 0.000 claims abstract description 18
- 230000033228 biological regulation Effects 0.000 claims abstract description 10
- 230000005389 magnetism Effects 0.000 claims abstract description 7
- 239000012535 impurity Substances 0.000 claims description 7
- 238000010521 absorption reaction Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 230000001276 controlling effect Effects 0.000 claims description 4
- 238000003795 desorption Methods 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 230000003247 decreasing effect Effects 0.000 claims description 2
- 230000001174 ascending effect Effects 0.000 claims 1
- 239000000243 solution Substances 0.000 description 18
- 238000001816 cooling Methods 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000004907 flux Effects 0.000 description 4
- 239000003124 biologic agent Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- 230000005672 electromagnetic field Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 230000031852 maintenance of location in cell Effects 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- UGKDIUIOSMUOAW-UHFFFAOYSA-N iron nickel Chemical compound [Fe].[Ni] UGKDIUIOSMUOAW-UHFFFAOYSA-N 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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Abstract
The invention discloses a kind of many cells magnetic separating method and system, method includes: to provide a variety of specific magnetic beads, and a variety of specificity magnetic beads are able in conjunction with plurality of target cell-specific, and the magnetism of a variety of specific magnetic beads is different；Make a variety of specific magnetic beads are corresponding with the plurality of target cell in cell mixing solution respectively to combine；The cell mixing solution for combining magnetic bead is passed into the cell sorting column in magnetic field and is sorted, wherein the intensity in regulation magnetic field, makes plurality of target cell successively be sorted out；Separation system includes cell sorting column, electromagnet, the conducting wire for electromagnet to be connected to power supply, is used to adjust current controller of the power supply to electromagnet supply current, cell sorting column is located in the magnetic field of electromagnet offer, and the electric current of power supply output is DC current and/or alternating current；Only single operation, which can be achieved, in the present invention can sort various kinds of cell, substantially increase the efficiency of separation and significantly reduce cost.
Description
Technical field
The invention belongs to cell sorting fields, and in particular to a kind of many cells magnetic separating method and system, it is especially suitable
For isolating various kinds of cell from cell mixing.
Background technique
Cell sorting refers to the operation that required specific cells are sub-elected from cell mixing solution.It is led in biology, medicine etc.
Domain, it is often necessary to which cell required for sub-electing from more complicated sample is to carry out various medical researches.
Currently, cell sorting method has centrifugal separation, fluorescence separating method and magnetic separating method etc., wherein magnetic separating method
Principle be to carry out combining target cell using specific magnetic bead, target cell is separated and operated using externally-applied magnetic field.Realize magnetic
The device of power sorting cell generally includes to provide the permanent magnet of externally-applied magnetic field and the internal sorting column filled with materials such as iron nickel,
Sorting column is placed in the magnetic field of permanent magnet offer.The process for carrying out cell sorting in the prior art is generally as follows: first will mixing
Cell solution is mixed with specific magnetic bead, makes target cell in conjunction with specific magnetic bead；Then solution is passed into sorting column,
It is adsorbed to the target cell in solution in conjunction with specific magnetic bead on sorting column and interior filler, and remaining cell is not inhaled
It is attached, it is removed through over cleaning；Sorting column is removed from magnetic field finally, is divided the target cell sorted on column again using dispersion liquid
It dissipates, and then obtains required cell.
It is compared with other methods, magnetic separating method has the clear superiorities such as high specificity, sharpness of separation height, however, as above
Shown in assorting room, existing method operates the sorting that can only carry out a kind of cell each time, and flux is lower.And it is past in practice
Toward needing to sub-elect various kinds of cell from cell mixing solution, just needs to carry out multi-pass operation at this time, do not only result in efficiency so
Lowly, cumbersome, and since sorting column is typically all that can not recycle, multi-pass operation just necessarily consumes multiple sortings
Column causes cost very high.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide, a kind of efficiency of separation is high, cost
Reduced many cells magnetic separating method.
The present invention also provides a kind of many cells used suitable for many cells magnetic separating method of the present invention point simultaneously
Select system.
In order to solve the above technical problems, a kind of technical solution that the present invention takes is as follows:
The invention discloses a kind of many cells magnetic separating methods, thin for sub-electing plurality of target from cell mixing solution
Born of the same parents, which comprises
A variety of specific magnetic beads are provided, a variety of specific magnetic beads are able to and the plurality of target cell-specific knot
It closes, and the magnetism of a variety of specific magnetic beads is different；
Make a variety of specific magnetic beads are corresponding with the plurality of target cell in the cell mixing solution respectively to combine；
The cell mixing solution for combining magnetic bead is passed into the cell sorting column in magnetic field and is sorted, wherein
The intensity for regulating and controlling the magnetic field makes the plurality of target cell successively be sorted out.
More according to the present invention specific and preferred aspect, the magnetic bead material of the specific magnetic bead not of the same race and/
Or magnetic bead volume is different.
More according to the present invention specific and preferred aspect, the magnetic bead volume differences of any two kinds specific magnetic beads
Absolute value be 125000 cubic nanometers ~ 0.027 cu μ m；Alternatively, lesser magnetic in any two kinds specific magnetic beads
The volume ratio of pearl and biggish magnetic bead be in 1:8 ~ 216 namely any two kinds of specific magnetic beads lesser magnetic bead with it is larger
Magnetic bead volume ratio be 1/216 ~ 1/8.
Some preferred aspects according to the present invention provide the magnetic field using the electromagnet of energization, and logical by control
Electric current realizes the regulation in the magnetic field, and the electrical current is modulated DC current and/or alternating current.
A specific aspect according to the present invention can be used alternating current and generate the electromagnetism that intensity follows current waveform
, in the variation of electromagnetic field degree of strength, the cell for being connected with magnetic bead can carry out adsorbing separation with magnetic field and adsorb following of separating again again
Ring effect, and while cell longitudinally flows downward along cell sorting column length axis direction, the ringing of this adsorbing separation
Residence time of the cell in cell sorting column is extended, so that impurity be made first to be separated cell sorting column, is connected with volume phase
It is short to the residence time of the cellular retention time opposite cell for being connected with the larger magnetic bead of volume compared with little magnetic bead, it is first flowed after impurity
Cell sorting column out is finally connected with the cell outflow of larger magnetic bead, can achieve once isolate not allogenic cell in this way
Purpose, and farthest reduce the time of physical absorption between cell and cell sorting column, reduce to the maximum extent
Destruction of the cell sorting column to cell.
Some preferred aspects according to the present invention, the waveform of the alternating current include but is not limited to be sinusoidal waveform, three
One of angle waveform and square waveform or a variety of combinations.
A specific aspect according to the present invention, the cell sorting column include filled with the iron for enhancing Distribution of Magnetic Field
And/or nickel.
More according to the present invention specific and preferred aspect, the method also includes: it controls the cell sorting and is in
It is carried out in the adjustable environment of temperature.
Some specific aspects according to the present invention, during carrying out the sorting, adjusting electrical current first makes whole
The plurality of target cell be adsorbed on the cell sorting column, after impurity to be removed, electric current is decreased to a setting value,
So that on cell sorting column with target cell desorption that magnetic the smallest magnetic bead connect and other target cells keep absorption, into
And obtain this kind of target cell, and so on, by target cell remaining on cell sorting column according to connected magnetic bead it is magnetic by
It is small to obtain corresponding target cell successively from desorption on cell sorting column to big sequence.
Some preferred aspects according to the present invention, the pre-set program for realizing the magnetic field regulation, certainly using program
The dynamic regulation for carrying out the magnetic field.
Some preferred aspects according to the present invention, Xiang Suoshu electromagnet provide alternating current, the frequency of the alternating current
Control is 0.01Hz ~ 100Hz.
Another technical solution provided by the invention: a kind of many cells magnetic separating system, the separation system include cell
Sorting column, the conducting wire for the electromagnet to be connected to power supply, is used to adjust the power supply and supplies to the electromagnet electromagnet
The current controller of electric current, the cell sorting column are located in the magnetic field of electromagnet offer, the electricity of the power supply output
Stream is DC current and/or alternating current.
Some preferred aspects according to the present invention, the separation system further include for electromagnet and/or cell sorting
Column carries out cooling cooling device.When the field strength of required electromagnetic field is too many, high current is needed, and then will cause heat release, therefore
Setting cooling device can keep sorting environment to be in the ideal state of temperature, to ensure that biological sample is not destroyed.
Some preferred aspects according to the present invention are set with the journey for realizing the magnetic field regulation in the current controller
Sequence, the electric current provided according to set procedure auto-control to electromagnet.
Some preferred aspects according to the present invention, the waveform of the alternating current are sinusoidal waveform, triangular waveform and rectangular
One of waveform or a variety of combinations.
Some preferred aspects according to the present invention, the frequency range of the alternating current are 0.01Hz ~ 100Hz.
Some preferred aspects according to the present invention, the separation system further include a variety of specific magnetic beads, a variety of spies
The magnetism of anisotropic magnetic bead is different.
It is further preferred that the magnetic bead material and/or magnetic bead volume of the specific magnetic bead not of the same race are different.
It is further preferred that the absolute value of the magnetic bead volume differences of any two kinds specific magnetic beads is 125000 cubes
Nanometer ~ 0.027 cu μ m；Alternatively, in any two kinds of specific magnetic beads lesser magnetic bead and biggish magnetic bead volume
Than being 1/216 for the volume ratio of lesser magnetic bead and biggish magnetic bead in 1:8 ~ 216 namely any two kinds of specific magnetic beads
~1/8。
In the present invention, specific magnetic bead refer to connection and/or be coated with specific antibody or receptor etc. can be with certain detail
The magnetic bead for the biomass that cell phase combines.If other class materials not only can in conjunction with specific cells but also can in conjunction with magnetic bead or.
Due to the use of above technical scheme, the invention has the following advantages over the prior art:
The method of the present invention and device can realize that only single operation can sort various kinds of cell, substantially increase the efficiency of separation and show
Work reduces cost.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of cell sorting system used in many cells magnetic separating method of the present invention；
Wherein, 1, current controller；2, conducting wire；3, cell sorting column；4, electromagnet；5, magnetic bead.
Specific embodiment
Improved many cells magnetic separating method can be realized and sub-elect different cells in same batch solution, and logical
Significant increase is measured, so that the various cells in solution are sufficiently separated and realize maximized utilization, while reducing valuableness
Biological agent cost.
By being placed in cell sorting column in the magnetic field using adjustable magnetic field intensity, and then can will have different magnetic strong
The magnetic bead of degree is connect with different types of cell, is passed through carefully when that will contain the mixed solution for being connected to the cell of different magnetic intensities
Born of the same parents sort column, can be by making the cell for being connected to different magnetic intensities according to magnetic to adjust the intensity in magnetic field with multiple waveforms
The magnetic intensity that pearl has successively flows out, to realize same batch cell solution while separate various kinds of cell, on the one hand improves
The utilization rate of cell, avoids wasting, and reduces expensive biological agent cost, while can also improve flux, enhancing separation effect
Rate especially reduces the physical damage to cell using the very big limit of adsorbing separation endless form, and also achieves cell sorting
The recycling of column, has saved cost.
Above scheme is described further below in conjunction with specific embodiment；It should be understood that these embodiments are for illustrating
The basic principles, principal features and advantages of the present invention, and the present invention is not by the scope limitation of following embodiment；It is used in embodiment
Implementation condition further adjustment can be done according to specific requirement, the implementation condition being not specified is usually the item in routine experiment
Part.
Embodiment 1
The present embodiment provides the examples that single separates two kinds of cells:
Target cell A and target cell B to be sorted is selected in same cell mixing solution, provide be able to it is described
Target cell specific binding two species specificity magnetic beads (for following simplicity, will be opposite with target cell A, target cell B respectively
The magnetic bead for the specific magnetic bead that should be combined is known as magnetic bead A and magnetic bead B), wherein the magnetism of magnetic bead A and magnetic bead B is different, in this example,
The diameter that magnetic bead A is arranged is 100 nanometers, and the diameter of setting magnetic bead B is the 200 nanometers of (magnetic force and magnetic of cell sorting column to magnetic bead
The volume of pearl is directly proportional)；
Make the combination corresponding with target cell A and target cell B respectively of two species specificity magnetic beads；
The cell mixing solution for combining magnetic bead is passed into the cell sorting column in magnetic field to sort, wherein regulating and controlling magnetic
The intensity of field, makes two kinds of target cells successively be sorted out；
Concrete operations are as follows: providing magnetic field in this example using the electromagnet being powered, and realize magnetic by control electrical current
The regulation of field intensity, specific electrical current select the DC current of controllable current size, and high current first makes to be passed into thin
Magnetic bead A and magnetic bead B in born of the same parents' sorting column are adsorbed to respectively on cell sorting column, then rinse cell sorting column using cleaning solution
And then it is washed out the impurity in the cell mixing solution for combining magnetic bead, weaken electric current later until magnetic lesser magnetic bead A
It is detached from absorption and magnetic biggish magnetic bead B is still attracted on cell sorting column, rinsing cell sorting column with dispersion liquid then can will
Magnetic bead A and the target cell A in conjunction with magnetic bead A are separated, and magnetic bead B and target cell B in conjunction with magnetic bead B be still at this time
So in the cell sorting column, electric current is then reduced to zero, cell sorting column disappears to the magnetic force of magnetic bead B, and then magnetic bead B is from thin
Born of the same parents sort to be detached from column and adsorb, and same available dispersion liquid rinses until magnetic bead B and the target cell B whole in conjunction with magnetic bead B
It is separated.
In this example, realizes same batch cell solution while separating various kinds of cell, on the one hand improve the utilization rate of cell,
It avoids wasting, reduces expensive biological agent cost, while flux can also be improved, enhance separative efficiency, while also realizing
The recycling of cell sorting column, has saved cost.
Embodiment 2
The present embodiment provides many cells magnetic separating system, separation system includes cell sorting column 3, electromagnet 4, is used for electromagnetism
Conducting wire 2 that iron 4 is connected to power supply, for adjust power supply to the current controller 1 of 4 supply current of electromagnet, for electromagnet 4
And/or cell sorting column 3 carries out cooling cooling device, cell sorting column 3 is located in the magnetic field of the offer of electromagnet 4, and power supply is defeated
Electric current out is alternating current, and the program for realizing magnetic field regulation is set in current controller 1, certainly according to set program
The electric current that dynamic control is provided to electromagnet 4.
In this example, the waveform of alternating current is sinusoidal waveform, and the frequency range of alternating current is 0.01Hz ~ 100Hz；
Preferably, separation system further includes a variety of specific magnetic beads, and the magnetism of a variety of specificity magnetic beads is different.Specifically,
The absolute value of the magnetic bead volume differences of any two kinds specific magnetic beads is 125000 cubic nanometers ~ 0.027 cu μ m；Or
Person, the volume ratio of lesser magnetic bead 5 and biggish magnetic bead 5 is 1:8 ~ 216 in any two kinds of specific magnetic beads, namely any
The volume ratio of lesser magnetic bead and biggish magnetic bead is 1/216 ~ 1/8 in two kinds of specific magnetic beads.
In this example, using sinusoidal waveform alternating current, it can be achieved that: electromagnetic field degree of strength change when, be connected with magnetic bead
Cell can carry out adsorbing separation with magnetic field and adsorb the ringing separated again again, and it is longitudinal long along cell sorting column 3 in cell
While axis direction flows downward, when the ringing of this adsorbing separation extends stop of the cell in cell sorting column 3
Between, so that impurity be made first to be separated cell sorting column 3, the cellular retention time for being connected with the relatively small magnetic bead of volume is connected with relatively
The residence time of the cell of the larger magnetic bead of volume is short, and cell sorting column 3 is first flowed out after impurity, is finally connected with larger magnetic bead
Cell outflow, can achieve once isolate the purpose of not allogenic cell in this way, and farthest reduce cell
The time of physical absorption between cell sorting column 3 reduces destruction of the cell sorting column 3 to cell, simultaneously to the maximum extent
The recycling of cell sorting column 3 is also achieved, has saved cost, while flux can also be improved, enhances separative efficiency.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention, it is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of many cells magnetic separating method, for sub-electing plurality of target cell from cell mixing solution, feature exists
In, which comprises
A variety of specific magnetic beads are provided, a variety of specific magnetic beads are able to and the plurality of target cell-specific knot
It closes, and the magnetism of a variety of specific magnetic beads is different；
Make a variety of specific magnetic beads are corresponding with the plurality of target cell in the cell mixing solution respectively to combine；
The cell mixing solution for combining magnetic bead is passed into the cell sorting column in magnetic field and is sorted, wherein
The intensity for regulating and controlling the magnetic field makes the plurality of target cell successively be sorted out.
2. many cells magnetic separating method according to claim 1, which is characterized in that the specific magnetic bead not of the same race
Magnetic bead material and/or magnetic bead volume it is different.
3. many cells magnetic separating method according to claim 1, which is characterized in that any two kinds specific magnetic beads
Magnetic bead volume differences absolute value be 125000 cubic nanometers ~ 0.027 cu μ m.
4. many cells magnetic separating method according to claim 1, which is characterized in that any two kinds specific magnetic beads
In the volume ratio of lesser magnetic bead and biggish magnetic bead be 1:8 ~ 216.
5. many cells magnetic separating method according to claim 1, which is characterized in that provided using the electromagnet being powered
The magnetic field, and the regulation in the magnetic field is realized by controlling electrical current, the electrical current is modulated direct current
Stream and/or alternating current.
6. many cells magnetic separating method according to claim 5, which is characterized in that the waveform of the alternating current is positive
One of string waveform, triangular waveform and square waveform or a variety of combinations.
7. many cells magnetic separating method according to claim 5, which is characterized in that during carrying out the sorting,
Adjusting electrical current first is adsorbed onto whole plurality of target cells on the cell sorting column, impurity to be removed
Afterwards, electric current is decreased to a setting value, so that the target cell desorption connecting on cell sorting column with magnetic the smallest magnetic bead
And other target cells keep absorption, and then obtain this kind of target cell, and so on, by target remaining on cell sorting column
Cell obtains corresponding target successively from desorption on cell sorting column according to the ascending sequence of the magnetism of connected magnetic bead
Cell.
8. according to many cells magnetic separating method described in claim 5 or 6 or 7, which is characterized in that pre-set realization institute
The program for stating magnetic field regulation carries out the regulation in the magnetic field using program automatically.
9. according to many cells magnetic separating method described in claim 5 or 6 or 7, which is characterized in that Xiang Suoshu electromagnet provides
The frequency control of alternating current, the alternating current is 0.01Hz ~ 100Hz.
10. a kind of many cells magnetic separating system, it is characterised in that: the separation system includes cell sorting column, electromagnet, use
In the conducting wire for being connected to the electromagnet with power supply, it is used to adjust current control of the power supply to the electromagnet supply current
Device, the cell sorting column are located in the magnetic field of electromagnet offer, the electric current of power supply output be DC current and/or
Alternating current.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112094839A (en) * | 2020-11-06 | 2020-12-18 | 深圳市赛特罗生物医疗技术有限公司 | Automatic cell magnetic sorting method and device |
| CN113058743A (en) * | 2021-03-30 | 2021-07-02 | 淮阴工学院 | Screening method and device for polydisperse magnetic polymer microspheres |
| CN116574585A (en) * | 2023-03-13 | 2023-08-11 | 中博瑞康(上海)生物技术有限公司 | A magnetic field device for magnetic cell separation and a method for magnetic cell separation |
| WO2025176223A1 (en) * | 2024-02-22 | 2025-08-28 | 妙顺（上海）生物科技有限公司 | Preparation method for functionalized nano magnetic beads |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN210176869U (en) * | 2018-04-12 | 2020-03-24 | 苏州天际创新纳米技术有限公司 | Multi-cell magnetic sorting system |
| CN112029662B (en) * | 2020-11-06 | 2021-02-05 | 深圳市赛特罗生物医疗技术有限公司 | Cell sorting magnetic grid structure, manufacturing method and magnetic grid tube |
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| CN210176869U (en) | 2020-03-24 |
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