CN119816720A - Magnetic bead mixing device - Google Patents
Magnetic bead mixing device Download PDFInfo
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- CN119816720A CN119816720A CN202280099798.2A CN202280099798A CN119816720A CN 119816720 A CN119816720 A CN 119816720A CN 202280099798 A CN202280099798 A CN 202280099798A CN 119816720 A CN119816720 A CN 119816720A
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- reagent bottle
- straight line
- limiting
- fixing member
- bottle fixing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
一种磁珠混匀装置，包括支撑机构(100)、驱动机构(200)和试剂瓶固定件(300)，支撑机构(100)具有容纳腔以及开口，容纳腔的中心线沿第一直线(20)延伸设置，驱动机构(200)的输出轴(201)能绕第一直线(20)进行旋转运动，试剂瓶固定件(300)设置为用于固定装有磁珠的试剂瓶(10)，试剂瓶固定件(300)通过开口置于容纳腔内，且试剂瓶固定件(300)的底端与输出轴(201)传动连接，试剂瓶固定件(300)的中心轴线沿第二直线(30)延伸设置。在驱动机构(200)的驱动下，试剂瓶固定件(300)能够以第二直线(30)绕第一直线(20)的周向移动的方式进行摆动，在摆动的过程中，第二直线(30)和第一直线(20)之间的夹角始终为α°，第二直线(30)的移动轨迹为圆锥面。
A magnetic bead mixing device comprises a support mechanism (100), a drive mechanism (200) and a reagent bottle fixing member (300), wherein the support mechanism (100) has a containing cavity and an opening, wherein the center line of the containing cavity is arranged to extend along a first straight line (20), an output shaft (201) of the drive mechanism (200) can rotate around the first straight line (20), and the reagent bottle fixing member (300) is arranged to fix a reagent bottle (10) containing magnetic beads, wherein the reagent bottle fixing member (300) is placed in the containing cavity through the opening, and the bottom end of the reagent bottle fixing member (300) is transmission-connected to the output shaft (201), and the center axis of the reagent bottle fixing member (300) is arranged to extend along a second straight line (30). Driven by the driving mechanism (200), the reagent bottle fixing member (300) can swing in a manner such that the second straight line (30) moves circumferentially around the first straight line (20). During the swinging process, the angle between the second straight line (30) and the first straight line (20) is always α°, and the moving trajectory of the second straight line (30) is a conical surface.
Description
The application relates to the technical field of biological sample extraction, in particular to a magnetic bead mixing device.
The magnetic bead extraction technique is widely used in experiments for extracting and purifying deoxyribonucleic acid (DeoxyriboNucleic Acid, DNA) and ribonucleic acid (Ribonucleic Acid, RNA). Before using magnetic beads to extract and purify DNA or RNA in biological samples, the magnetic beads in the reagent bottle and the solution are required to be uniformly mixed, so that silicon hydroxyl groups on the surfaces of the magnetic beads can be specifically combined with nucleic acid in the solution through hydrogen bonding and electrostatic action. The magnetic beads can be combined with nucleic acid under the high-salt condition, and the nucleic acid combined with the magnetic beads can be eluted under the low-salt condition, so that the nucleic acid can be directly separated from a complex biological system by utilizing the principle.
The automatic magnetic bead mixing method adopted by gene sequencing is mainly based on a suction method. The suction method is to add the magnetic beads into a reagent bottle filled with a sample in advance, and then repeatedly suck and wash the magnetic beads through a pipetting gun and a pipetting needle to achieve the effects of elution and uniform mixing. However, when the method is applied to the library construction of a long fragment library of gene sequencing, the magnetic beads on the side wall of the reagent bottle are not easy to wash off in the sucking and beating mixing process, so that the magnetic beads are seriously aggregated, and the magnetic beads are aggregated on the side wall of the reagent bottle, so that the elution and mixing becomes relatively difficult.
Disclosure of Invention
The application provides a magnetic bead mixing device, which ensures that magnetic beads are prevented from agglomerating on the side wall of a reagent bottle, and is beneficial to improving the effect and efficiency of biological sample extraction.
The application provides a magnetic bead mixing device which comprises a supporting mechanism, a driving mechanism and a reagent bottle fixing piece, wherein the supporting mechanism is provided with a containing cavity, an opening is formed in the top of the supporting mechanism, the central line of the containing cavity is arranged in an extending mode along a first straight line, the driving mechanism comprises an output shaft capable of rotating around the first straight line, the reagent bottle fixing piece is used for fixing a reagent bottle with magnetic beads, the reagent bottle fixing piece is arranged in the containing cavity through the opening, the bottom end of the reagent bottle fixing piece is in transmission connection with the output shaft, the central axis of the reagent bottle fixing piece is arranged in an extending mode along a second straight line, the second straight line is intersected with the first straight line, the reagent bottle fixing piece can swing in a mode that the second straight line moves around the circumference of the first straight line, and in the swing process, an included angle between the second straight line and the first straight line is always alpha degrees, and a moving track of the second straight line is a conical surface.
FIG. 1 is a schematic structural diagram of a magnetic bead mixing device according to an embodiment of the present application;
FIG. 2 is an enlarged view of portion B of FIG. 1;
FIG. 3 is a schematic view of a magnetic bead mixing device according to an embodiment of the present application, partially cut away;
FIG. 4 is a cross-sectional view of a magnetic bead mixing device according to an embodiment of the present application;
Fig. 5 is a schematic view of an eccentric driving shaft of a magnetic bead mixing device according to an embodiment of the present application.
In the figure:
100. The device comprises a supporting mechanism, 101, a base, 102, a supporting column, 103, a motor base, 104, a bearing seat, 105, a bearing end cover, 106, a first chamber, 107 and a second chamber;
200. 201, output shaft;
300. reagent bottle fixing piece 301, test tube cavity 302, tubular part 303, taper part 304, connecting column;
400. The device comprises an eccentric driving shaft, 401, a plug hole, 402, a mounting hole, 403 and an induction piece;
500. A limiting mechanism; 501, a first limiting piece, 502, a second limiting piece;
600. a third limiting member;
700. a first knuckle bearing;
800. A second knuckle bearing;
900. An inductive sensor;
110. deep groove ball bearings;
120. clamping springs;
10. reagent bottle, 20, first straight line, 30, second straight line.
The technical solutions of the present application will be described below with reference to the accompanying drawings, and the described embodiments are some of the embodiments of the present application.
In the description of the present application, the positional or positional relationship indicated by the terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present application and simplifying the description, and do not indicate or imply that the apparatus or element in question must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.
In the description of the present application, unless specified and limited otherwise, the terms "mounted," "connected," and "coupled" are used broadly, and may be used in a fixed or removable manner, in a mechanical or electrical connection, in a direct or indirect connection via an intermediary, or in a communication between two elements. The meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to circumstances.
The application provides a magnetic bead mixing device which is used for mixing magnetic beads in a reagent bottle uniformly, so that the magnetic beads are prevented from being accumulated on the side wall of the reagent bottle, and extraction and purification of biological macromolecules such as DNA, RNA and the like in a biological sample are prevented from being influenced.
As shown in fig. 1 to 4, the magnetic bead mixing device includes a support mechanism 100, a driving mechanism 200, and a reagent bottle holder 300. The support mechanism 100 is an installation structure of the whole magnetic bead mixing device, and the driving mechanism 200 and the reagent bottle fixing member 300 are both installed on the support mechanism 100. The support mechanism 100 has a receiving cavity that forms an opening at the top of the support mechanism 100, the centerline of the receiving cavity extending along the first line 20. In some embodiments, the first line 20 is disposed vertically. The driving mechanism 200 is a power member of the whole magnetic bead mixing device, the driving mechanism 200 comprises an output shaft 201, and the output shaft 201 can perform rotary motion by taking the first straight line 20 as a rotary shaft. The reagent bottle fixing member 300 is a member for fixing the reagent bottle 10, the reagent bottle fixing member 300 is disposed in the accommodating chamber through the opening, and the bottom end of the reagent bottle fixing member 300 is in transmission connection with the output shaft 201, the central axis of the reagent bottle fixing member 300 extends along the second straight line 30, and the second straight line 30 and the first straight line 20 intersect, that is, the second straight line 30 and the first straight line 20 have an intersection point.
The reagent bottle holder 300 is driven by the driving mechanism 200 to swing in such a manner that the second straight line 30 moves circumferentially around the first straight line 20, and in the swinging process of the reagent bottle holder 300, the included angle between the second straight line 30 and the first straight line 20 is always α °, and the moving track of the second straight line 30 is a conical surface. Since the reagent bottle 10 is fixed to the reagent bottle holder 300, the reagent bottle 10 and the sample and the magnetic beads in the reagent bottle 10 can swing. Centrifugal force is formed in the reagent bottle 10 in the swinging process of the reagent bottle 10, the magnetic beads can be evenly mixed with the sample in a vibrating mode under the action of the centrifugal force, the magnetic beads cannot be condensed on the side wall of the reagent bottle 10, and the biological sample extraction effect and efficiency are improved.
In some embodiments, the angle α is 1 °,2 °, or 3 °, which is set to prevent the magnetic beads from being accumulated on the sidewall of the reagent bottle 10, and to prevent the sample in the reagent bottle 10 from being spilled from the opening of the reagent bottle 10 under the action of centrifugal force. In other embodiments of the application, the value of α° can be flexibly set according to the requirements.
With continued reference to fig. 2-5, the magnetic bead mixing device further comprises an eccentric drive shaft 400, with which eccentric drive shaft 400 a driving connection of the reagent bottle holder 300 to the output shaft 201 of the drive mechanism 200 can be achieved. The eccentric driving shaft 400 is disposed in the accommodating cavity, the bottom end of the eccentric driving shaft 400 is connected with the output shaft 201, the eccentric driving shaft 400 can rotate around the first straight line 20 under the driving of the driving mechanism 200, the top end of the eccentric driving shaft 400 is provided with a mounting hole 402, the distance between the central axis of the mounting hole 402 and the first straight line 20 is A, and the bottom end of the reagent bottle fixing piece 300 is connected in the mounting hole 402 in a swinging manner. In the embodiment of the application, the value of the interval A is not limited, and can be flexibly set according to requirements.
In some embodiments, the receiving cavity includes a first chamber 106 and a second chamber 107, the eccentric drive shaft 400 is rotatably disposed within the first chamber 106, and a deep groove ball bearing 110 is disposed between an outer wall surface of the eccentric drive shaft 400 and an inner wall surface of the first chamber 106. In some embodiments, to achieve the limitation of the deep groove ball bearing 110, a stepped surface is formed on the inner wall surface of the first chamber 106, and a shoulder is formed on the outer wall surface of the eccentric drive shaft 400, with the deep groove ball bearing 110 being limited between the stepped surface and the shoulder.
In some other embodiments, in addition to using an eccentric drive shaft 400 to achieve a driving connection of the reagent bottle holder 300 to the output shaft 201 of the drive mechanism 200, a planetary gear mechanism may be used to achieve a driving connection of the reagent bottle holder 300 to the output shaft 201 of the drive mechanism 200, with the sun gear of the planetary gear mechanism being connected to the output shaft 201 of the drive mechanism 200 and the planetary gears of the planetary gear mechanism being in oscillating connection with the bottom end of the reagent bottle holder 300.
With continued reference to fig. 3 to 4, in order to enable the eccentric drive shaft 400 to precisely oscillate at a set angle, the magnetic bead mixing apparatus further includes a first knuckle bearing 700, the first knuckle bearing 700 including an outer ring having an inner spherical surface and an inner ring having an outer spherical surface, the inner ring being swingably disposed within the outer ring. The first knuckle bearing 700 is disposed in the mounting hole 402, and an outer ring of the first knuckle bearing 700 is fixedly disposed with respect to the mounting hole 402, and a center axis of the outer ring of the first knuckle bearing 700 coincides with a center axis of the mounting hole 402. The bottom end of the reagent bottle holder 300 is formed with a connection post 304, and the connection post 304 is inserted into the inner ring of the first knuckle bearing 700.
With continued reference to fig. 3, the magnetic bead mixing device further includes a second knuckle bearing 800, where the second knuckle bearing 800 includes an outer ring and an inner ring, the outer ring has an inner spherical surface, the inner ring has an outer spherical surface, and the inner ring is swingably disposed in the outer ring. The second knuckle bearing 800 is placed in the accommodating cavity and is arranged close to the opening, the outer ring of the second knuckle bearing 800 is fixedly arranged in the accommodating cavity, the central axis of the outer ring of the second knuckle bearing 800 coincides with the first straight line 20, and the reagent bottle fixing piece 300 passes through the inner ring of the second knuckle bearing 800. The center axis of the inner ring of the first knuckle bearing 700 and the center axis of the inner ring of the second knuckle bearing 800 always coincide with the second straight line 30.
With continued reference to fig. 2, the magnetic bead mixing device further includes a limiting mechanism 500, where the limiting mechanism 500 is configured to limit the reagent bottle holder 300, so that the reagent bottle holder 300 can only swing. The limiting mechanism 500 includes a first limiting member 501 and a second limiting member 502, the first limiting member 501 is disposed on the top surface of the supporting mechanism 100, the second limiting member 502 is disposed on the outer wall surface of the reagent bottle fixing member 300, and the second limiting member 502 and the first limiting member 501 are cooperatively disposed to limit the reagent bottle fixing member 300 to rotate around the second straight line 30.
In some embodiments, the first limiting member 501 is a limiting plate, and a limiting groove is disposed on the limiting plate. For example, the limiting plate is a U-shaped plate, the U-shaped plate surface of the U-shaped plate is perpendicular to the radial direction of the reagent bottle fixing member 300, the top of the U-shaped groove is open, and the U-shaped groove penetrates the limiting plate in the radial direction of the reagent bottle fixing member 300. The second limiting member 502 includes a limiting shaft and a limiting bearing, the limiting shaft is convexly arranged outside the reagent bottle fixing member 300 along the radial direction of the reagent bottle fixing member 300, the limiting bearing is sleeved on the limiting shaft, and the limiting bearing is movably arranged in the limiting groove. Because the inner wall of the U-shaped groove has a certain limiting function on the limiting bearing, the limiting bearing arranged in the U-shaped groove can rotate around the limiting shaft and can swing up and down in the U-shaped groove, but cannot move out of the U-shaped groove.
In some embodiments, the second stop 502 is a cam follower. The cam follower is a standard component, can be directly purchased and is convenient to use.
With continued reference to fig. 1, 3, 4 and 5, the magnetic bead mixing device further includes a detection mechanism, where the detection mechanism includes an induction piece 403 and an induction sensor 900, the induction piece 403 is disposed on the eccentric driving shaft 400, the induction sensor 900 is disposed on the supporting mechanism 100, and when the driving mechanism 200 is reset to the origin, the induction sensor 900 detects the induction piece 403.
In some embodiments, as shown in fig. 4 and 5, the sensing tab 403 is integrated directly onto the eccentric drive shaft 400. The sensing piece 403 is an annular convex edge formed on the outer wall surface of the eccentric driving shaft 400, a through groove is formed at the outer edge of the annular convex edge, the sensing sensor 900 comprises a transmitting end and a receiving end which are oppositely arranged, and the transmitting end and the receiving end are respectively arranged on the upper side and the lower side of the annular convex edge and are opposite to the through groove. When the through groove rotates to a position between the transmitting end and the receiving end, the light emitted by the transmitting end can be received by the receiving end, and at the moment, the driving mechanism 200 is reset to the original point, while when the through groove rotates to other positions, the other parts of the annular convex edge block the light emitted by the transmitting end, so that the receiving end cannot receive the light, and at the moment, the driving mechanism 200 is not reset to the original point. The sensing sensor 900 and the sensing piece 403 can count the number of rotations of the output shaft 201 of the driving mechanism 200, thereby facilitating control of the swing duration of the reagent bottle 10.
In some parallel embodiments, the sensing piece 403 and the eccentric driving shaft 400 are two independent components, and the sensing piece 403 may have a ring structure, so that the sensing piece 403 can be sleeved on the eccentric driving shaft 400 and fixed, and the fixing manner may be welding, connecting piece connection or clamping connection, etc. The sensing piece 403 may also be a circular plate, and the sensing piece 403 is fixedly connected to an end surface of the eccentric driving shaft 400, for example, an upper end surface or a lower end surface, and the fixing direction of the sensing piece 403 and the eccentric driving shaft 400 includes, but is not limited to, welding, connecting piece connection or clamping connection.
In some embodiments, the drive mechanism 200 includes a closed-loop motor with a motor shaft that is the output shaft 201. The closed-loop motor can realize closed-loop control, so that high-rotation-speed and high-precision control can be realized.
In some side-by-side embodiments, the drive mechanism 200 includes a stepper motor with an output shaft 201 as the motor shaft.
In some parallel embodiments, the drive mechanism 200 includes a stepper motor and a timing belt mechanism, wherein a motor shaft of the stepper motor is connected with a driving pulley of the timing belt mechanism, and a driven pulley shaft of the timing belt mechanism is an output shaft 201.
With continued reference to fig. 4, the reagent bottle holder 300 defines a test tube cavity 301 along the second line 30, and the reagent bottle 10 is inserted into the test tube cavity 301. The arrangement ensures that the sample in the reagent bottle 10 does not need to be contacted in the mixing process, so that the occurrence of the phenomenon of cross contamination of the sample can be avoided.
The reagent bottle 10 comprises a bottle body and a bottle cap, wherein the bottle body is conical, and the bottle cap is connected to an opening of the bottle body through a bendable connecting sheet. In order to avoid that the reagent bottle 10 placed in the test tube cavity 301 is accidentally separated from the test tube cavity 301, and as shown in fig. 2, the reagent bottle fixing member 300 is provided with a third limiting member 600, and the third limiting member 600 is configured to be clamped with a bottle cap of the reagent bottle 10. In some embodiments, the third limiting member 600 is a U-shaped piece, and the connecting piece on the bottle cap is clamped in the U-shaped through groove of the U-shaped piece.
In some embodiments, the support mechanism 100 is a split structure that is made up of multiple components. With continued reference to fig. 1, the support structure includes a base 101, a support column 102, a motor base 103, a bearing pedestal 104, and a bearing end cover 105, which are sequentially connected from bottom to top, where the connection manner includes, but is not limited to, welding, connection by a connecting piece, or magnetic attraction.
In some embodiments, the base 101 is a rectangular plate. The number of the support columns 102 is plural, the plurality of support columns 102 are uniformly distributed between the base 101 and the motor base 103, and a space for installing the driving mechanism 200 is formed between the plurality of support columns 102. In some embodiments, the number of support columns 102 is four, with four support columns 102 disposed at four corners of the base 101, respectively. The number of support columns 102 can be flexibly adjusted according to the requirements. The motor base 103 is internally provided with a first chamber 106 in a stepped shape, the bearing base 104 is internally provided with a second chamber 107 communicated with the first chamber 106, and the second chamber 107 is also in a stepped shape. A part of the structure of the eccentric drive shaft 400 is arranged in the first chamber 106, the other part is arranged in the second chamber 107, the deep groove ball bearing 110 is arranged in the first chamber 106, and the output shaft 201 of the drive mechanism 200 extends into the first chamber 106 to be connected with the bottom end of the eccentric drive shaft 400. The bearing end cover 105 is connected at the top of the bearing seat 104, the second knuckle bearing 800 is arranged in the second chamber 107 and is close to the opening, the outer ring of the second knuckle bearing 800 is limited between the step surface of the second chamber 107 and the bearing end cover 105, and the bottom of the inner ring of the second knuckle bearing 800 is limited by the clamp spring 120 sleeved on the reagent bottle fixing piece 300. The bearing cap 105 has an annular structure, and an inner circumferential surface thereof forms an opening of the support mechanism 100.
Examples:
The following describes a magnetic bead mixing device according to an embodiment of the present application with reference to fig. 1 to 5.
As shown in fig. 1 to 5, the magnetic bead mixing device includes a support mechanism 100, a driving mechanism 200, a reagent bottle holder 300, an eccentric drive shaft 400, a first joint bearing 700, a second joint bearing 800, a deep groove ball bearing 110, a limiting mechanism 500, and a detecting mechanism.
The supporting mechanism 100 comprises a base 101, a supporting column 102, a motor base 103, a bearing pedestal 104 and a bearing end cover 105 which are sequentially connected from bottom to top. The base 101 is the rectangular plate, and the bottom of motor cabinet 103 is provided with annular chimb along circumference, and the quantity of support column 102 is four, and four support columns 102 set up respectively in four corners of base 101, and the bottom and the base 101 fixed connection of support column 102, the top fixed connection of support column 102 are on the annular chimb of motor shaft. The motor cabinet 103 is connected with the bearing pedestal 104 through a connecting piece, a first chamber 106 is arranged on the motor cabinet 103 in a penetrating mode along the vertical direction, a second chamber 107 is arranged on the bearing pedestal 104 in a penetrating mode along the vertical direction, and the first chamber 106 and the second chamber 107 are communicated and are all stepped chambers. The bearing end cover 105 is a rectangular annular plate, a circular opening is formed in the bearing end cover 105 in a penetrating mode, the bearing end cover 105 is arranged on the bearing seat 104 in a covering mode, and the bearing end cover 105 covers the top opening of part of the second chamber 107. The first chamber 106, the second chamber 107 and the inner bore of the bearing end cap 105 form a receiving cavity of the support mechanism 100, and a central axis of the receiving cavity is the first straight line 20.
The driving mechanism 200 is a closed-loop motor, the closed-loop motor is arranged between the base 101 and the motor base 103 and is positioned between the four support columns 102, a motor shaft of the closed-loop motor is an output shaft 201 of the driving mechanism 200, and the motor shaft of the closed-loop motor extends into the first chamber 106.
The eccentric driving shaft 400 is rotatably connected in the accommodating cavity of the supporting mechanism 100 through the deep groove ball bearing 110, the central axis of the eccentric driving shaft 400 is a second straight line 30, and the included angle between the second straight line 30 and the first straight line 20 is alpha deg.. The top end of the eccentric drive shaft 400 is placed in the second chamber 107 of the bearing block 104, the bottom end of the eccentric drive shaft 400 is placed in the first chamber 106 of the motor block 103, and a shaft shoulder is formed on the outer wall surface of the eccentric drive shaft 400. The deep groove ball bearing 110 is disposed in the first chamber 106 and sleeved on the eccentric driving shaft 400, and the deep groove ball bearing 110 forms a limit in the vertical direction through the shaft shoulder of the eccentric driving shaft 400 and the stepped surface in the first chamber 106. The bottom end of the eccentric driving shaft 400 is provided with a plug hole 401 along the vertical direction, the top end of the eccentric driving shaft 400 is provided with a mounting hole 402 along the setting direction, and the distance between the central axis of the plug hole 401 and the central axis of the mounting hole 402 is A. The motor shaft of the closed-loop motor is inserted into the plug hole 401 from bottom to top, the first joint bearing 700 is arranged in the mounting hole 402, the first joint bearing 700 comprises an outer ring and an inner ring, the outer ring is provided with an inner spherical surface, the inner ring is provided with an outer spherical surface, the inner ring is arranged in the outer ring in a swinging manner, the central axis of the outer ring of the first joint bearing 700 coincides with the central axis of the mounting hole 402, and the central axis of the inner ring of the first joint bearing 700 coincides with the second straight line 30. The second joint bearing 800 is disposed in the second chamber 107, the reagent bottle fixing member 300 is disposed through an inner ring of the second joint bearing 800, a central axis of an outer ring of the second joint bearing 800 coincides with the first straight line 20, a central axis of an inner ring of the second joint bearing 800 coincides with the second straight line 30, and the inner ring of the second joint bearing 800 is limited by a clamp spring 120 sleeved on the reagent bottle fixing member 300.
The reagent bottle holder 300 includes a tubular portion 302, a tapered portion 303, and a connecting post 304 connected in this order, a large-sized end of the tapered portion 303 is connected to a bottom end of the tubular portion 302, and a small-sized end of the tapered portion 303 is connected to a top end of the connecting post 304. The connection post 304 is inserted into the inner race of the first knuckle bearing 700. The tubular part 302 is provided with a test tube cavity 301, and the reagent bottle 10 is inserted into the test tube cavity 301. The reagent bottle 10 comprises a bottle body and a bottle cap, wherein the bottle body is conical, and the bottle cap is connected to an opening of the bottle body through a bendable connecting sheet. The reagent bottle fixing member 300 is provided with a third limiting member 600, and the third limiting member 600 is configured to be engaged with a bottle cap of the reagent bottle 10.
The limiting mechanism 500 comprises a first limiting piece 501 and a second limiting piece 502, wherein the first limiting piece 501 is arranged on the top surface of the supporting mechanism 100, the first limiting piece 501 is a U-shaped limiting plate, and a U-shaped limiting groove is formed in the limiting plate. The U-shaped plate surface of the limiting plate is perpendicular to the radial arrangement of the reagent bottle fixing piece 300, the top of the U-shaped limiting groove is open, and the limiting groove penetrates through the limiting plate in the radial direction of the reagent bottle fixing piece 300. The second limiting piece 502 is arranged on the outer wall surface of the reagent bottle fixing piece 300, the second limiting piece 502 is a cam follower, and a bearing of the cam follower is arranged in the limiting groove.
The detection mechanism comprises a sensing piece 403 and a sensing sensor 900, wherein the sensing piece 403 is arranged on the eccentric driving shaft 400, the sensing sensor 900 is arranged on the bearing seat 104, and when the closed motor is reset to the original point, the sensing sensor 900 detects the sensing piece 403.
Claims (10)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2022/141816 WO2024138293A1 (en) | 2022-12-26 | 2022-12-26 | Magnetic bead mixing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN119816720A true CN119816720A (en) | 2025-04-11 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202280099798.2A Pending CN119816720A (en) | 2022-12-26 | 2022-12-26 | Magnetic bead mixing device |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN119816720A (en) |
| WO (1) | WO2024138293A1 (en) |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7108651B2 (en) * | 2001-04-20 | 2006-09-19 | Hitachi Koko Co., Ltd. | Centrifuge having multiple rotors |
| JP4144582B2 (en) * | 2004-10-04 | 2008-09-03 | ダイキン工業株式会社 | Mixing apparatus and fluorine concentration measuring system and method using the same |
| CN203275173U (en) * | 2013-04-16 | 2013-11-06 | 深圳迈瑞生物医疗电子股份有限公司 | Reaction liquid mixing device and chemical analysis meter |
| CN209086048U (en) * | 2018-11-16 | 2019-07-09 | 安图实验仪器(郑州)有限公司 | Fixed reagent evenly mixing device for in-vitro diagnosis equipment |
| CN110770589A (en) * | 2019-07-18 | 2020-02-07 | 烟台艾德康生物科技有限公司 | Tubular luminous reagent oscillation and mixing device and method |
| CN211864772U (en) * | 2019-11-14 | 2020-11-06 | 迈克医疗电子有限公司 | Blending device |
| CN211927487U (en) * | 2020-01-20 | 2020-11-13 | 深圳迈瑞生物医疗电子股份有限公司 | Sample mixing device |
| CN113588393B (en) * | 2021-07-30 | 2023-04-28 | 中元汇吉生物技术股份有限公司 | Sample mixing mechanism |
| CN215428637U (en) * | 2021-08-14 | 2022-01-07 | 北京指真生物科技有限公司 | Automatic mixing mechanism of code microsphere |
| CN216433694U (en) * | 2021-12-03 | 2022-05-03 | 精匠诊断技术(江苏)有限公司 | Peripheral blood mixing mechanism |
| CN114272820B (en) * | 2021-12-31 | 2023-09-01 | 中元汇吉生物技术股份有限公司 | Mixing Mechanism and Sample Analyzer |
-
2022
- 2022-12-26 CN CN202280099798.2A patent/CN119816720A/en active Pending
- 2022-12-26 WO PCT/CN2022/141816 patent/WO2024138293A1/en not_active Ceased
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| Publication number | Publication date |
|---|---|
| WO2024138293A1 (en) | 2024-07-04 |
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