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CN1193470A - Yeast for producing fodder from stalks and preparation thereof - Google Patents

Yeast for producing fodder from stalks and preparation thereof Download PDF

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Publication number
CN1193470A
CN1193470A CN97100990A CN97100990A CN1193470A CN 1193470 A CN1193470 A CN 1193470A CN 97100990 A CN97100990 A CN 97100990A CN 97100990 A CN97100990 A CN 97100990A CN 1193470 A CN1193470 A CN 1193470A
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kilograms
straw
quyi
sort
starch
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李正祥
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Tianmen Patented Technologies Application Co
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Tianmen Patented Technologies Application Co
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Abstract

A bionic stalk (straw) feed suitable for non-ruminant animals is prepared through such technological steps as making yeast with microbes including endorumen one, mixing it with dried powdered stalks or straws, nutrients (trace elements and vitamines) and water and natural stacking at 18-40 deg.C for 4-6 hr, and features no need of anaerobic fermentation, high nutritive value, and saving cost by about 30%.

Description

Make sort of quyi of bionical producting stalk fodder and preparation method thereof
The present invention relates to a kind ofly produce sort of quyi that feed uses and preparation method thereof and with the method for this sort of quyi making feed; Non-preparation method of ruminating the producting stalk fodder of livestock and poultry particularly is suitable for feeding.
Make producting stalk fodder and adopt chemical methodes such as liquefied ammonia ammonification and urea ammoniated usually, need to consume a large amount of ammoniacal liquor and urea, cost is higher, and the feed of making can only be used to feed ruminants such as ox, sheep, and if the excessive edible intoxicating phenomenon that then easily produces.
The method that adopts rumen content ferment making producting stalk fodder is also arranged, " making the method and the special equipment of rumen feed " disclosed as CN1061891A, this method is by extracting fresh rumen content, cultivate koji, admix and ferment in the lime treated straw raw material, make producting stalk fodder, but need the straw alkalization and still will add ammoniacal liquor to ferment, complex process, cost are still higher.
CN1101673A disclosed " microorganism fermenting compound fungus and be used for the method that the fermenting crop straw is produced feed stripped " adopts microorganism fungus kind to make producting stalk fodder fully, need not add ammoniacal liquor, lime, technology is simpler, cost is lower, but this microbial composite bacteria is compared with rumen content, and it is single that bacterial classification also shows.
More than several microorganism producting stalk fodders make ferment making feed under anaerobic, fermentation time is longer, and cellulose resolution ratio and protein content are still waiting to improve in the feed.
The objective of the invention is to overcome the deficiency of above-mentioned producting stalk fodder preparation agent and preparation method, and provide a kind of easy and simple to handle, Production Time is short, bionical producting stalk fodder sort of quyi of the microorganism that cost is low and preparation method thereof and the method for making bionical producting stalk fodder of the fresh bionical producting stalk fodder of this sort of quyi or making.
The object of the present invention is achieved like this: a kind of bionical producting stalk fodder sort of quyi, comprise the microorganism infusorian in the cud, the capillus worm, the bistrichiasis worm, interior caterpillar, Bacteroides succinogenes, your pupil's fiber clostridium, yellow Ruminococcus, Ruminococcus albus, little Ruminobacter, bargen's streptococcus, clostridium butyricum, the lonely bacterium of butyric acid, ruminate beastly semilune monad, Bacillus acidi lactici, streptococcus lactis, the starch coccus, the starch sarcine, the starch spirillum, little living flavobacterium vitarumen, in the sort of quyi, also contain following microorganism: saccharomycete, cellulose-decomposing bacteria, mould, basidiomycetes, equal caterpillar, the fine hair worm, autotrophic bacterium, heterotroph, various microorganism proportionings are as follows in the sort of quyi: (gram contains the bacterium number)
The bistrichiasis worm, interior caterpillar each 4,500,000,000--5,000,000,000
Starch coccus, starch sarcine, starch spirillum each 1,000,000,000--1,500,000,000
Basidiomycetes, little living flavobacterium vitarumen each 600,000,000--800,000,000
Other microorganism each 400,000,000--600,000,000.
Mentioned microorganism can be wooden at field soil, rotten wood, bacterium, extract in the ruminant body or the body surface collection is cultivated, and market or micro-organisms unit also can buy.
The method of making bionical producting stalk fodder of the sort of quyi is: with straw powder or the short straw of hand hay cutter, adding gram by the 0.5%-0.7% of straw dried bean noodles material, to contain the bacterium number be 20,000,000,000--25,000,000,000 the sort of quyi and with the nutritional agents of preparations such as various trace elements, vitamin and water in proportion mix evenly the back at 18 ℃--under 40 ℃ of temperature, directly throw something and feed after 4--6 hour livestock and poultry or oven dry of stacking deposited naturally.Wherein the straw powder or the straw of hand hay cutter weak point and the proportioning of the sort of quyi and water are: 100: 0.5--0.7: 100--150;
Composition and the content of making the used nutritional agents of bionical producting stalk fodder adding comprise: (restraining/100 kilograms of straw siccatives)
Lysine 80-120 methionine 80-120
Choline Chloride 200-250 sodium selenite 6-12
Nicotinic acid 60-100 pantothenic acid 30-70
Vitamin A 20-40 vitamin D 5-10
Vitamin E 50-80 terramycin powder 10-20
Copper sulphate 3.5-4 manganese sulfate 3.5-4
Ferrous sulfate 10-20 calcium carbonate 6-12
Cobalt chloride 0.35-0.4 magnesium sulfate 5-6
Zinc sulfate 6-12 boric acid 6-12
Prokeyvit 20-30 vitamin B1 8-10
Vitamin B6 4-5 vitamin B2 25-30
Cobalamin 35 millis-40 millis
Make in the short straw of the straw powder of bionical producting stalk fodder or hand hay cutter and can add grain by-products such as oily chaff, wheat bran, its addition can be the straw siccative heavy 20%-35%.
The producting stalk fodder of the making livestock and poultry of directly to throw something and feed, the also available fresh feed that is made into remakes the sort of quyi, by adding 5 kilograms at least in 100 kilograms of straw dried bean noodles material, when surpassing 5 kilograms, should have additional nutrients in the ratio of above given nutritional agents and the water quantity of agent and water guarantees nutritional agents and the content of water in formulation, after mixing thoroughly, under 18-40 ℃ of temperature, stacked 4-6 hour naturally, directly throw something and feed or dry and deposit; But number of repetition should not surpass 5 times, for guaranteeing the nutrition comprehensive utilization ratio of feed, also can add the former sort of quyi again and make, and guarantees that it is 20,000,000,000 that its gram contains the bacterium number--25,000,000,000.
This specification also discloses a kind of method of making the bionical producting stalk fodder sort of quyi, after the various microorganisms that are about to need to contain in the sort of quyi are extracted in and cultivate on the culture medium, be inoculated in following original seed: sorghum flour, rice bran, corn, the carclazyte powder, fish meal, rice bran, the cake class makes up in the batching of making by percentage, as 10 kilograms of sorghum flours, 20 kilograms in rice bran, 7.5 kilograms of corns, 5 kilograms in carclazyte powder, 1 kilogram of fish meal, 5 kilograms in rice bran, 1.5 kilograms of cake classes, and add above-mentioned nutritional agents and water in proportion and place in 18-20 ℃ the greenhouse, the control temperature raises in 2 ℃ every day, reach 35 ℃-38 ℃ until room temperature, water content is 12%-14%.Extraction, the cultured method of various microorganisms see embodiment for details.
The invention has the advantages that:
1, the sort of quyi adopts the microorganism cultivation to form fully, bacterial classification is wide in variety, not only comprise the anaerobe in the cud, also has the natural oxygen consumption microorganism of part, decomposition, the saccharification of straw fiber have been quickened, and be converted into mycoprotein and various active enzyme by a large amount of breedings of microorganism, the producting stalk fodder nutritive value is obviously improved.
2, preparation method is simple, convenient, and fermentation time is short, does not need straw is made basification, does not also need the closing anaerobic fermentation, and the feed making cost is low; And the fresh bionical producting stalk fodder of making also can be used for making this feed as the sort of quyi again, has greatly made things convenient for the culturist.
3, the feed of making has been rich in plurality of enzymes, multiple acid and alcohols fragrance pungent, and soft, fragrant and sweet, little acid, slightly fruit and vinosity, nutritious, good palatability, livestock and poultry are liked to eat.Viable bacteria in the feed enters in livestock and poultry stomach and the enteron aisle not only to help after back and beneficial microbe are within it joined and digests and assimilates, and also produces abundant nutrition, and it is feeding not only to be suitable for ruminating livestock and poultry, and is suitable for that non-to ruminate livestock and poultry feeding.
The present invention is further illustrated below in conjunction with embodiment:
At first from cud, field soil, rotten wood, ruminant body surface belly etc. locates to extract equal caterpillar, the fine hair worm, the capillus worm, the bistrichiasis worm, interior caterpillar, Bacteroides succinogenes, your pupil's fiber clostridium, yellow Ruminococcus, Ruminococcus albus, little Ruminobacter, bargen's streptococcus, butyric acid rib bacterium, the butyric acid vibrios, ruminate beastly semilune monad, the starch coccus, the starch sarcine, the starch spirillum, the flavobacterium vitarumen of supporting one's family, infusorian, saccharomycete, mould, cellulose-decomposing bacteria, basidiomycetes, Bacillus acidi lactici, streptococcus lactis, autotrophic bacterium, microorganisms such as heterotroph, these microorganisms also can be from market or micro-organisms unit buy.Its cultural method is: 1) to the cultivation of the microorganism of microorganism in the cud and collection.Promptly extract rumen content, this can ruminate from the ox slaughtered, sheep etc. and extract the livestock stomach, also can adopt stomach tube to extract or extracts from the puncture of ox belly with fistula; From field soil, rotten wood, bacterium are wooden, gather other required microorganism.2) microorganism with rumen content and collection places the triangular flask of sterilization to add aqua sterilisa, leaves standstill after swaying evenly, gets its supernatant, and being diluted to concentration with aqua sterilisa is 10 -4, 10 -5, 10 -6Suspension.3) mixedly avoid liquid with what the sterilization suction pipe was drawn variable concentrations, be inoculated on the broth agar culture medium after match holder filter filters, anaerobism was cultivated 2-3 days in 25 ℃-37 ℃.4) under electron microscope,, from the back culture medium, hook up desirable worm section respectively by required microbe species with steam sterilizing transfer needle, ring, Eimeria and bacterial strain, be inoculated on the semi-solid agar medium separately, under 25 ℃ of-37 ℃ of temperature, anaerobism is separated cultivation 2-3 days.5) the various microorganisms of separate cultivating look breeding and growing state and can repeat third step 1--3 time again, pure until the microorganism kind of turning out, young tender, the reproductive capacity of living is strong, the density height.6) with the bacterial classification of cultivating, by the given scope preparation of contained various microorganisms in the sort of quyi, be modulated into every milliliter of liquid that contains viable bacteria 2,000,000,000 with aqua sterilisa again, evenly sow again in the sort of quyi raw material for preparing in advance, by raw material: water=1: 1-1.5 stirs after adding water and adding the nutritional agents of given range above, places in 18 ℃-20 ℃ the greenhouse, and the control temperature raises in 2 ℃ every day, reach 35 ℃-38 ℃ up to room temperature, get the sort of quyi when water content is 12%-14%; Mentioned microorganism nutritional agents, water be because of given its dosage range, so, multiple combination can appear, as long as containing the bacterium number, the sort of quyi of making gram reaches 20,000,000,000--and 25,000,000,000, all can the result of use of the sort of quyi not produced a very large impact.
Clean straw is made the shape straw powder of cracking rice with pulverizer, or cut up with a hay cutter into the straw of 5-15 millimeter, said here straw comprises the straw of straw, wheat, corn, jowar, beans, the woody plant cauline leaf of corncob, peanut shell and nontoxic free from extraneous odour.Can add grain by-products such as oily chaff, wheat bran at straw powder or the short straw of hand hay cutter, its addition is the 25%-30% of straw material weight, can certainly make bionical feed with this class cereal product, and just the cost height is not calculated.
By following component and content preparation nutritional agents (restraining/100 kilograms of straw siccatives)
Lysine, methionine each 80, choline 220, nicotinic acid 80, pantothenic acid 50,3-Hydroxyretinol 0, Vitamin D5, vitamin E 60, terramycin powder 15, copper sulphate 3.5, ferrous sulfate 15, calcium carbonate, zinc sulfate, boric acid each 8, sodium selenite 10, manganese sulfate 3.5, the public cobalt 0.35 of chlorine, magnesium sulfate 5, Cobastab 18, Cobastab 225, Cobastab 64, Cobastab 1235 millis, vitamin K 320.
Then, straw powder or the short straw of hand hay cutter are added gram, and to contain the bacterium number be 20,000,000,000-25,000,000,000 the sort of quyi, addition adds the 500-700 gram by hundred kilograms, add the nutritional agents and the 1-1.5 water doubly that prepare again, mix evenly back is stacked naturally and is connect throw something and feed livestock and poultry or oven dry after 4-6 hour certainly and deposit under 10 ℃～40 ℃ temperature; Also fresh bionical feed can be added 5 kilograms at least by hundred kilograms of straw siccatives, the quantity of should have additional nutrients according to quantity when surpassing 5 kilograms agent and water keeps nutritional agents and the content of water in formulation.Make bionical producting stalk fodder method with reference to the sort of quyi and make bionical producting stalk fodder.
The producting stalk fodder that employing the present invention makes is through the sem image scanning, and its coarse fiber structure is loose, all degradeds of the tight part of rough surface and structure in the basic stitch; Part is degraded to low molecular weight carbohydrate and can be reassembled into new free amino acid and a large amount of mycoprotein, the loose part that stays is the original structure tight section, a large amount of thalline stretch into organization internal, mainly in the solid content superficial growth, abundant hydrolase just can act on this, and can promote gastrointestinal motility, reduce enteron aisle viscosity; Abundant hydrolase system is arranged in the producting stalk fodder of making, as amylase, cellulase, hemicellulase, phytase, tartaric acid enzyme, protease, lactase etc. under fragrant pungent effects such as various enzymes and various acid, alcohol, soft, fragrant and sweet, the little acid of producting stalk fodder, slightly fruit and aroma, palatability improve greatly, and mouthfeel and nutritive value are near grain feed.The invention sort of quyi is made bionical feeds such as all kinds of straw powder, grass meal, oily chaff, and its crude protein and crude fiber content relatively see the following form:
Figure A9710099000091
Bionical producting stalk fodder do nursing hybridized pig, the lean meat species growing and fattening pigs test made from the sort of quyi of the present invention and preparation method show, increase about 10% than routine feeding full price grain concentrated feed appetite, the weightening finish piece of getting fat, the live pig weight ratio routine that increases day by day is raised pigs and is increased by 0.2 to 0.4 kilogram, pig feed with back skin red tender, hair is glossy, quiet like sleep, premunition is strong.This feed ratio full price concentrated feed 25%-35% that reduces cost.

Claims (6)

1, a kind of sort of quyi of making bionical producting stalk fodder, comprise bovine rumen interior microorganism infusorian, capillus worm, bistrichiasis worm, interior caterpillar, Bacteroides succinogenes, your pupil's fiber clostridium, yellow Ruminococcus, Ruminococcus albus, little Ruminobacter, bargen's streptococcus, clostridium butyricum, butyric acid vibrios, ruminate beastly semilune monad, Bacillus acidi lactici, streptococcus lactis, starch coccus, starch sarcine, starch spirillum, little living flavobacterium vitarumen, it is characterized in that:
(1) in the sort of quyi, also contains saccharomycete, cellulose-decomposing bacteria, mould, basidiomycetes, equal caterpillar, fine hair worm, autotrophic bacterium, heterotroph;
(2) various microorganism proportionings are as follows in the sort of quyi: (gram contains the bacterium number)
Bistrichiasis worm, interior caterpillar each 4,000,000,000-5,000,000,000;
Starch coccus, starch sarcine, starch spirillum each 1,000,000,000-1,500,000,000;
Basidiomycetes, little living flavobacterium vitarumen each 600,000,000-800,000,000;
Other microorganism each 400,000,000-600,000,000.
2, a kind of method of making bionical producting stalk fodder is characterized in that: get straw powder or the short straw of hand hay cutter, and even with the sort of quyi, nutritional agents and water mix, after stacking 4-6 hour naturally under 18～40 ℃ of temperature, directly throw something and feed or dry and deposit; Divide three steps to carry out:
(1) addition of the sort of quyi is to add 500～700 grams in 100 kilograms of straw siccatives;
(2) component of nutritional agents and addition are; (restraining/100 kilograms of straw siccatives)
Lysine 80～120 methionine 80～120
Choline Chloride 200～250 sodium selenites 6～12
Nicotinic acid 60～100 pantothenic acid 30～70
Vitamin A 20～40 vitamin Es 50～80
Vitamin D 5～10 terramycin powders 10～20
Ferrous sulfate 10～20 copper sulphate 3.5～4
Manganese sulfate 3.5～4 calcium carbonate 6～12
Cobalt chloride 0.35～0.4 magnesium sulfate 5～6
Zinc sulfate 6～12 boric acid 6～12
Vitamin K 320～30 Cobastabs 18～10
Cobastab 64～5 Cobastabs 225～30
Cobastab 1235 millis～40 millis
(3) addition of water is heavy 1～1.5 times of straw siccative.
3, a kind of fresh bionical producting stalk fodder of making according to claim 2 method of making bionical producting stalk fodder, its feature are in fourth: the addition of the fresh bionical producting stalk fodder of making is to add 5 kilograms at least in per hundred kilograms of straw siccatives; When surpassing 5 kilograms, should be by claim 2 to the have additional nutrients quantity of agent and water of certainty ratio.
4, preparation method according to claim 2 is characterized in that: add the grain by-product powder in the straw of straw powder or hand hay cutter weak point, its addition is 20%～35% of a straw siccative weight.
5, a kind of method of making the described bionical producting stalk fodder sort of quyi of claim 1, it is characterized in that to contain the described various microbial inoculants of claim 1 in the batching that sorghum flour, rice bran, corn, carclazyte powder, starch, rice bran, cake class are made by the percentage combination, and add described nutritional agents of claim 2 and water according to quantity, place in 18 ℃～20 ℃ the greenhouse, the control temperature raises in 2 ℃ every day, reach 35 °～38 ℃ until room temperature, water content is 12%～14%.
6, method according to claim 5, the prescription that it is characterized in that its batching are 10 kilograms of sorghum flours, 20 kilograms in rice bran, 7.5 kilograms of corns, 5 kilograms in carclazyte powder, 1 kilogram of starch, 5 kilograms in rice bran, 1.5 kilograms of cake classes.
CN97100990A 1997-03-19 1997-03-19 Yeast for producing fodder from stalks and preparation thereof Pending CN1193470A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1324129C (en) * 2004-04-27 2007-07-04 包头市天赋食品有限公司 Multiple strain microorganism ferment production method and its uses in cow fine fodder
CN101679984A (en) * 2007-05-29 2010-03-24 安迪苏法国联合股份有限公司 RumC peptide with antimicrobial activity
CN1620900B (en) * 2004-12-16 2010-04-21 云兆雪 Artificial rumen leaven
CN101773204A (en) * 2010-03-12 2010-07-14 唐承培 Traditional Chinese medicine viable bacteria feed master batch and preparation method thereof and method by using master batch to prepare feed
CN101229196B (en) * 2008-01-14 2011-04-20 河北师范大学 Microecologic agent preventing grice diarrhoea and preparing method thereof
CN104431415A (en) * 2014-10-28 2015-03-25 亿利资源集团有限公司 Active microorganism feed for ruminant animals

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1324129C (en) * 2004-04-27 2007-07-04 包头市天赋食品有限公司 Multiple strain microorganism ferment production method and its uses in cow fine fodder
CN1620900B (en) * 2004-12-16 2010-04-21 云兆雪 Artificial rumen leaven
CN101679984A (en) * 2007-05-29 2010-03-24 安迪苏法国联合股份有限公司 RumC peptide with antimicrobial activity
CN101679984B (en) * 2007-05-29 2013-08-07 安迪苏法国联合股份有限公司 Rumc peptides with antimicrobial activity
CN101229196B (en) * 2008-01-14 2011-04-20 河北师范大学 Microecologic agent preventing grice diarrhoea and preparing method thereof
CN101773204A (en) * 2010-03-12 2010-07-14 唐承培 Traditional Chinese medicine viable bacteria feed master batch and preparation method thereof and method by using master batch to prepare feed
CN104431415A (en) * 2014-10-28 2015-03-25 亿利资源集团有限公司 Active microorganism feed for ruminant animals

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