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CN118837181B - Flow type fluorescence detection pretreatment equipment - Google Patents

Flow type fluorescence detection pretreatment equipment Download PDF

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Publication number
CN118837181B
CN118837181B CN202411328700.3A CN202411328700A CN118837181B CN 118837181 B CN118837181 B CN 118837181B CN 202411328700 A CN202411328700 A CN 202411328700A CN 118837181 B CN118837181 B CN 118837181B
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fixed
plate
reciprocating
sampling
shaped
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CN118837181A (en
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赵海涛
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Changzhou Genmag Biotechnology Co ltd
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Changzhou Genmag Biotechnology Co ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38Diluting, dispersing or mixing samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state

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  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hydrology & Water Resources (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

本发明适用于取样检测技术领域,提供了一种流式荧光检测前处理设备,包括摇匀组件,摇匀组件内部滑动配合有试样组件,摇匀组件包括支架件以及滑动配合在其内部的往复件,试样组件包括铰接配合在往复件上定位件,若干插接固定在定位件内部的试管件以及滑动配合在定位件底部的取样件,该设备解决了现有的流式荧光检测前处理设备在使用时,不能够对分别储存在多个试管内部的多份待检测液体进行取样前的摇匀震荡,导致试管内部的待检测液体溶液难以充分的进行取样前的反应,另外,现有的设备摇匀震荡结构过于复杂,且难以在进行摇匀震荡过后及时的对待检测液体进行取样操作,对后期的检测结果造成了一定的影响的问题。

The present invention is applicable to the technical field of sampling detection, and provides a flow fluorescence detection pre-treatment device, including a shaking component, a sample component slidably fitted inside the shaking component, the shaking component including a bracket component and a reciprocating component slidably fitted inside the shaking component, the sample component including a positioning component hingedly fitted on the reciprocating component, a plurality of test tube components plugged and fixed inside the positioning component, and a sampling component slidably fitted at the bottom of the positioning component. The device solves the problem that the existing flow fluorescence detection pre-treatment device cannot shake and oscillate multiple portions of liquid to be detected stored in multiple test tubes before sampling, which makes it difficult for the liquid solution to be detected in the test tube to fully react before sampling. In addition, the shaking and oscillating structure of the existing device is too complicated, and it is difficult to sample the liquid to be detected in time after shaking and oscillating, which has a certain impact on the later detection results.

Description

Flow type fluorescence detection pretreatment equipment
Technical Field
The invention relates to the technical field of sampling detection, in particular to a flow type fluorescence detection pretreatment device.
Background
The flow fluorescent detection technology is an analysis method widely applied to the biomedical field, combines cytology and optical technology, and is used for rapidly and accurately analyzing and identifying cells, particles and biomolecules; in the detection process, fluorescent coding microspheres are used as cores, and a plurality of technologies such as a current collecting principle, laser analysis, high-speed digital signal processing and the like are integrated into a multi-index parallel analysis technology platform, so that 100 different biomolecules can be accurately and quantitatively detected at one time; can be used for various aspects and fields of research such as immunoassay, nucleic acid research, enzymology analysis, receptor, ligand recognition analysis and the like.
When the flow type fluorescence detection is carried out, shaking and vibrating reaction treatment operation is needed before sampling the liquid to be detected, however, the following technical problems exist in the prior flow type fluorescence detection pretreatment equipment when in use:
That is, in the process that current flow type fluorescence detection pretreatment equipment is being used, be difficult to reach the test tube and shake the process at the inside reciprocal of oscillating of height formula of equipment, can not store respectively in the inside many liquid that wait to detect of a plurality of test tubes shake before taking a sample, lead to the inside reaction before waiting to detect liquid solution of test tube to be difficult to abundant the sample, the testing procedure after taking a sample to it leads to the fact the influence, in addition, current equipment shakes shake the structure too complicated, and be difficult to shake the even shake after timely wait to detect liquid and sample the operation, have caused certain influence to the testing result of later stage.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide the flow type fluorescence detection pretreatment equipment which can achieve the high-low reciprocating vibration process of the whole positioning piece in the support frame, uniformly shake the liquid to be detected before sampling the liquid to be detected stored in the plurality of test tube pieces respectively, ensure that the liquid solution to be detected in the test tube pieces can be uniformly mixed and vibrated respectively, fully perform the reaction before sampling and avoid the influence on the later sampling detection process.
In order to achieve the above purpose, the present invention provides the following technical solutions:
The flow type fluorescence detection pretreatment equipment comprises a shaking component, wherein a sample component is slidably matched in the shaking component.
The shaking-up assembly comprises a support piece and a reciprocating piece in the shaking-up assembly in a sliding fit mode, the sample assembly comprises a positioning piece in the reciprocating piece in a hinged fit mode, a plurality of test tube pieces in the positioning piece in a plugging mode and sampling pieces in the bottom of the positioning piece in a sliding fit mode.
The support piece comprises a support frame, two opposite outer side surfaces of the support frame are respectively provided with an oblique groove in a penetrating mode, two symmetrical vertical plates are respectively fixed on the two opposite outer side surfaces of the support frame, and a U-shaped guide plate is respectively fixed on one side of each vertical plate, located above the support frame.
The reciprocating piece comprises a U-shaped bottom plate which is in sliding fit between two U-shaped guide plates, first pin shafts are fixed on two opposite inner side walls of the U-shaped bottom plate, first hinge arms are in hinge fit with the peripheral side faces of the first pin shafts, second hinge arms are fixed on two opposite inner side walls of the U-shaped bottom plate, second hinge arms are in hinge fit with the peripheral side faces of the second pin shafts, and first hinge holes and second hinge holes are respectively formed in one sides of the first hinge arms and one side of the second hinge arms in a penetrating mode.
The locating piece comprises a U-shaped sliding plate, third pin shafts which are in hinged fit with the first hinge holes are fixed on two opposite outer side surfaces of the U-shaped sliding plate, fourth pin shafts which are in hinged fit with the second hinge holes are fixed on two opposite outer side surfaces of the U-shaped sliding plate, and the third pin shafts are in sliding fit with the oblique grooves.
The invention is further provided with: the outer bottom of the support frame is fixedly provided with a servo motor, an output shaft of the servo motor is positioned in the support frame and is fixedly provided with a reciprocating arm, and the top of the reciprocating arm is fixedly provided with a reciprocating rod.
A vertical plate is fixed on the outer side face of the U-shaped bottom plate, a reciprocating plate is fixed at the bottom of the vertical plate, and a reciprocating groove which is in sliding fit with the peripheral side face of the reciprocating rod is formed in the top of the reciprocating plate in a penetrating mode.
The invention is further provided with: limiting grooves are formed in the two opposite inner sides of the U-shaped sliding plate, a movable bottom plate is slidably matched between the limiting grooves, symmetrical two side baffles are fixed at the top of the movable bottom plate, a top sealing plate is hinged between the two side baffles, and a handle rod is fixed at the top of the top sealing plate.
And a sealing rubber cover plate is fixed at the bottom of the top sealing plate.
The invention is further provided with: the center position of one side of the top sealing plate is fixed with a hinge seat, a rotating plate is hinged and matched inside the hinge seat through a torsion spring, one side of the rotating plate is fixed with a positioning plate, and one side of the positioning plate is fixed with a plurality of protruding blocks.
And a plurality of grooves matched with the protruding blocks are formed in one side of the movable bottom plate.
The invention is further provided with: two symmetrical connecting plates are fixed at the bottoms of the U-shaped sliding plates, an installation bottom plate is fixed at the bottoms of the two connecting plates together, a driving motor is fixed at the top of the installation bottom plate, an eccentric wheel is fixed on an output shaft of the driving motor, and a rotating groove is formed in the top of the eccentric wheel.
The bottom of the movable bottom plate is fixedly provided with a sliding rod which is in sliding fit with the inside of the rotary groove.
The invention is further provided with: the top of the movable bottom plate is communicated with a plurality of sleeves.
The test tube piece is including pegging graft the test tube of cooperation in the sleeve is inside, test tube periphery side is fixed with the holding ring of laminating each other with the removal bottom plate top.
The utility model discloses a test tube, including test tube, sealing gasket, clamping fit, sliding rod, sealing gasket, clamping fit and sealing gasket, the test tube bottom intercommunication is provided with the play appearance pipe of inwards extending, the inside slip of going out appearance pipe is provided with the slide bar, the slide bar top is fixed with the rubber seal in the test tube inside, the rubber seal bottom is fixed with the joint cooperation at the ring channel at play appearance pipe top, the slide bar diameter is less than the internal diameter of going out appearance pipe.
The bottom of the sliding rod is fixed with a positioning circular plate, and an elastic spring is fixed between the positioning circular plate and the sampling tube.
The invention is further provided with: and a plurality of guide rods are fixed at the bottom of the movable bottom plate.
The sampling piece includes the template, template bottom intercommunication is provided with a plurality of sampling tubes, sampling tube bottom is fixed with the touch pole, a plurality of sliding fit's guiding hole on the guiding rod have been seted up in the template top penetration.
The invention is further provided with: two symmetrical supporting plates are fixed at the bottom of the movable bottom plate, a telescopic cylinder is fixed on one side of the supporting plate, a positioning cylinder is fixed at the telescopic end of the telescopic cylinder, a T-shaped plate is fixed on one side of the positioning cylinder, a rotating column is fixed on one side of the T-shaped plate, a lifting arm is rotatably matched with the peripheral side face of the rotating column, a push-pull rod is fixed on one side of the lifting arm, and an inclined panel which is mutually attached to the lifting arm is fixed below the rotating column on one side of the T-shaped plate.
The lug plates are fixed on two opposite side surfaces of the sample plate, the side plates are fixed on the bottoms of the lug plates, and displacement grooves which are in sliding fit with the push-pull rods are formed in one side of each side plate.
The invention has the advantages that:
1. According to the invention, through the oblique reciprocating movement process of the whole positioning piece in the support frame, the high-low type reciprocating vibration process of the whole positioning piece in the support frame is achieved, and the preliminary shaking vibration process before sampling is carried out on the multiple liquid to be detected stored in the multiple test tube pieces respectively, so that the components in the liquid solution to be detected in the test tube pieces can be preliminarily mixed, vibrated uniformly, and the reaction before sampling is sufficiently carried out preliminarily, and the influence on the later sampling detection process is avoided.
2. According to the invention, the driving motor is started to drive the eccentric wheel fixed on the output shaft of the driving motor to rotate, so that the rotating groove formed in the top of the eccentric wheel slides on the peripheral side surface of the sliding rod, a reciprocating acting force is provided for the sliding rod, and the movable bottom plate is driven to reciprocate horizontally between the two limiting grooves, so that the liquid to be detected in the test tube is further subjected to horizontal reciprocating shaking operation, the mixing degree of the liquid to be detected in the test tube is further increased, and the influence of the liquid to be detected in the test tube on the later sampling detection process is avoided.
3. According to the invention, through the upward lifting rotation of the lifting arm on the peripheral side surface of the rotating column, an upward moving acting force is provided for the sample discharging plate, so that the abutting rod fixed at the bottom in the sampling tube is gradually close to the bottom of the positioning circular plate, and meanwhile, an upward moving acting force is provided for the positioning circular plate, the elastic spring is compressed, so that the annular groove is gradually separated from the top of the sample discharging tube, the liquid to be detected after shaking uniformly in the test tube can flow out from the inside of the sample discharging tube and finally falls into the inside of the sampling tube, and the shaking sampling process before detection is performed.
4. According to the invention, through the continuous reverse movement process of the two telescopic cylinders, the push-pull rod and the displacement groove are driven to gradually separate, the compressed elastic spring starts to elastically reset, and the sliding rod is driven to move downwards, so that the annular groove formed in the bottom of the rubber sealing gasket gradually approaches to the top of the sampling tube, the top opening end of the test tube is sealed and plugged, and the phenomenon that liquid to be detected after the residual part in the test tube is uniformly shaken in the process of sampling in the later period is prevented from dripping from the test tube, so that the detection environment is polluted is avoided.
Drawings
FIG. 1 is a schematic diagram of a flow-type fluorescence detection pretreatment device according to the present invention.
Fig. 2 is a schematic structural diagram of the shaking-up assembly of the present invention.
FIG. 3 is a schematic diagram of a sample assembly according to the present invention.
Fig. 4 is a front view of a sample assembly of the present invention.
Fig. 5 is a schematic structural view of the bracket member of the present invention.
Fig. 6 is a front view of the bracket member of the present invention.
Fig. 7 is a schematic structural view of the shuttle of the present invention.
Fig. 8 is a front view of the shuttle of the present invention.
Fig. 9 is a schematic structural view of the positioning member of the present invention.
Fig. 10 is a top view of the positioning member of the present invention.
Fig. 11 is a front view of the positioning member of the present invention.
Fig. 12 is a side view of a positioning member of the present invention.
FIG. 13 is a schematic structural view of the test tube of the present invention.
FIG. 14 is a schematic cross-sectional view of a test tube according to the present invention.
Fig. 15 is a schematic structural view of a sample member according to the present invention.
FIG. 16 is a schematic cross-sectional view of a sample member according to the present invention.
In the figure: 1. shaking up the assembly; 2. a sample assembly; 3. a bracket member; 4. a shuttle; 5. a positioning piece; 6. a test tube; 7. sampling a sample piece; 301. a support frame; 302. an inclined groove; 303. a vertical plate; 304. u-shaped guide plates; 305. a servo motor; 306. a reciprocating arm; 307. a reciprocating lever; 401. a U-shaped bottom plate; 402. a first pin; 403. a first articulated arm; 404. a second pin; 405. a second articulated arm; 406. a first hinge hole; 407. a second hinge hole; 408. a riser; 409. a shuttle plate; 410. a reciprocating groove; 501. a U-shaped slide plate; 502. a third pin; 503. a fourth pin; 504. a limit groove; 505. a movable bottom plate; 506. side baffles; 507. a top sealing plate; 508. a handle bar; 509. sealing the rubber cover plate; 510. a hinge base; 511. a rotating plate; 512. a positioning plate; 513. a protruding block; 514. a groove; 515. a connecting plate; 516. a mounting base plate; 517. a driving motor; 518. an eccentric wheel; 519. a rotating groove; 520. a slide bar; 521. a sleeve; 522. a guide rod; 523. a support plate; 524. a telescopic cylinder; 525. a positioning cylinder; 526. a T-shaped plate; 527. rotating the column; 528. a lifting arm; 529. a push-pull rod; 530. a bevel panel; 601. a test tube; 602. a positioning ring; 603. a sampling tube; 604. a slide bar; 605. a rubber gasket; 606. an annular groove; 607. positioning a circular plate; 608. an elastic spring; 701. setting a template; 702. a sampling tube; 703. a touch-up rod; 704. a guide hole; 705. ear plates; 706. a side panel; 707. and a displacement groove.
Detailed Description
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The application will be described in detail below with reference to the drawings in connection with embodiments.
It is noted that all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs unless otherwise indicated.
In the present invention, unless otherwise indicated, the terms "upper" and "lower" are used generally with respect to the directions shown in the drawings, or with respect to the vertical, vertical or gravitational directions; also, for ease of understanding and description, "left, right" is generally directed to the left, right as shown in the drawings; "inner and outer" refer to inner and outer relative to the outline of the components themselves, but the above-described orientation terms are not intended to limit the present invention.
Referring to fig. 1-16, the present invention provides the following technical solutions:
The flow type fluorescence detection pretreatment equipment comprises a shaking component 1, wherein a sample component 2 is in sliding fit with the inside of the shaking component 1; the shaking-up assembly 1 comprises a bracket component 3 and a reciprocating component 4 which is in sliding fit in the shaking-up assembly, the sample assembly 2 comprises a positioning component 5 which is in hinged fit on the reciprocating component 4, a plurality of test tube components 6 which are inserted and fixed in the positioning component 5 and a sampling component 7 which is in sliding fit at the bottom of the positioning component 5; the bracket piece 3 comprises a supporting frame 301, wherein inclined grooves 302 are formed in the opposite outer side surfaces of the supporting frame 301 in a penetrating manner, two symmetrical sets of vertical plates 303 are fixed on the opposite outer side surfaces of the supporting frame 301, and a U-shaped guide plate 304 is fixed on one side of each set of vertical plates 303 above the supporting frame 301; the reciprocating member 4 comprises a U-shaped bottom plate 401 which is in sliding fit between two U-shaped guide plates 304, wherein first pin shafts 402 are fixed on two opposite inner side walls of the U-shaped bottom plate 401, first hinge arms 403 are in hinged fit on the peripheral side surfaces of the first pin shafts 402, second pin shafts 404 are fixed on two opposite inner side walls of the U-shaped bottom plate 401, second hinge arms 405 are in hinged fit on the peripheral side surfaces of the second pin shafts 404, and first hinge holes 406 and second hinge holes 407 are respectively formed in one sides of the first hinge arms 403 and the second hinge arms 405 in a penetrating manner; the positioning piece 5 comprises a U-shaped sliding plate 501, a third pin shaft 502 which is in hinged fit with the first hinge hole 406 is fixed on two opposite outer sides of the U-shaped sliding plate 501, a fourth pin shaft 503 which is in hinged fit with the second hinge hole 407 is fixed on two opposite outer sides of the U-shaped sliding plate 501, and the third pin shaft 502 is in sliding fit with the inclined groove 302.
The specific application of the first embodiment is as follows:
After the whole equipment is installed and the preparation action of the liquid to be detected is completed, the U-shaped bottom plate 401 is driven to reciprocate between the two U-shaped guide plates 304 through the reciprocating movement process of the U-shaped bottom plate 401 in the support frame 301, when the U-shaped bottom plate 401 reciprocates between the two U-shaped guide plates 304, the hinging and rotating fit of the first hinging arm 403 between the first pin shaft 402 and the third pin shaft 502 and the hinging and rotating fit of the second hinging arm 405 between the second pin shaft 404 and the fourth pin shaft 503 are realized through the hinging and rotating fit of the second hinging arm 405 between the second pin shaft 404 and the fourth pin shaft 503, and the oblique sliding process of the U-shaped sliding plate 501 in the two oblique grooves 302 is combined, so that the oblique reciprocating sliding process of the U-shaped sliding plate 501 in the U-shaped bottom plate 401 is realized, the oblique reciprocating movement process of the whole positioning piece 5 in the support frame 301 is realized, the high-low reciprocating vibration process of the whole positioning piece 5 in the support frame 301 is realized, and then the multiple test tube pieces 6 which are positioned and fixed in the positioning piece 5 are synchronously subjected to the high-low reciprocating vibration process, the multiple test tube pieces 6 are respectively stored in the positioning piece 6 are subjected to preliminary vibration process before the sampling, the multiple test tube pieces 6 are subjected to preliminary vibration test sample test liquid, the test sample liquid can be mixed, the test liquid can be completely, and the test sample liquid can be mixed, and the test sample liquid can be completely.
Referring to fig. 1-16, in the second embodiment, a servo motor 305 is fixed at the bottom of the outer side of the support 301, a reciprocating arm 306 is fixed at the output shaft of the servo motor 305 inside the support 301, and a reciprocating rod 307 is fixed at the top of the reciprocating arm 306; a vertical plate 408 is fixed on the outer side surface of the U-shaped bottom plate 401, a reciprocating plate 409 is fixed at the bottom of the vertical plate 408, and a reciprocating groove 410 which is in sliding fit with the peripheral side surface of the reciprocating rod 307 is formed in the top of the reciprocating plate 409 in a penetrating manner; limiting grooves 504 are formed in the two opposite inner sides of the U-shaped sliding plate 501, a movable bottom plate 505 is slidably matched between the two limiting grooves 504, symmetrical two side baffles 506 are fixed at the top of the movable bottom plate 505, a top sealing plate 507 is hinged and matched between the two side baffles 506, and a handle rod 508 is fixed at the top of the top sealing plate 507; a sealing rubber cover plate 509 is fixed at the bottom of the top sealing plate 507; a hinge seat 510 is fixed at the center of one side of the top sealing plate 507, a rotating plate 511 is hinged and matched inside the hinge seat 510 through a torsion spring, a positioning plate 512 is fixed on one side of the rotating plate 511, and a plurality of protruding blocks 513 are fixed on one side of the positioning plate 512; a plurality of grooves 514 matched with the raised blocks 513 are formed on one side of the movable bottom plate 505; two symmetrical connecting plates 515 are fixed at the bottom of the U-shaped sliding plate 501, a mounting bottom plate 516 is jointly fixed at the bottom of the two connecting plates 515, a driving motor 517 is fixed at the top of the mounting bottom plate 516, an eccentric wheel 518 is fixed on an output shaft of the driving motor 517, and a rotating groove 519 is formed at the top of the eccentric wheel 518; a sliding rod 520 which is in sliding fit with the inside of the rotating groove 519 is fixed at the bottom of the movable bottom plate 505; the top of the movable bottom plate 505 is provided with a plurality of sleeves 521 in a communicating manner; the test tube 6 comprises a test tube 601 which is inserted and matched in the sleeve 521, and a positioning ring 602 which is mutually attached to the top of the movable bottom plate 505 is fixed on the peripheral side surface of the test tube 601.
The specific application of the second embodiment is as follows:
When the whole device is used for shaking up and sampling before liquid to be detected is detected, firstly, a rotating plate 511 which is hinged and matched with a torsion spring is pressed in a hinging seat 510, so that the rotating plate 511 is tilted, a plurality of protruding blocks 513 arranged on one side of a movable bottom plate 505 are driven to be separated from a plurality of grooves 514 respectively, then, a top sealing plate 507 which is hinged and matched between two side baffles 506 is rotated, so that the top sealing plate 507 is turned by 180 degrees, a plurality of test tubes 601 are sequentially and respectively inserted into a plurality of sleeves 521, positioning rings 602 fixed on the peripheral side surfaces of the plurality of test tubes 601 are sequentially and respectively attached to the top of the movable bottom plate 505, the movable insertion process of the test tube 6 is completed, after the movable insertion process is finished, the liquid to be detected before mixing shaking up is respectively in a plurality of groups, and the liquid to be sequentially added into the plurality of test tubes 601, so that shaking up and sampling operations before later detection can be performed;
After the above operation is completed, the top sealing plate 507 hinged and matched between the two side baffles 506 is started to reversely rotate, so that the top sealing plate 507 is reversely turned by 180 degrees, the sealing rubber cover plate 509 fixed at the bottom of the top sealing plate 507 is finally attached to the tops of the test tubes 601 fixed at the positions, the sealing rubber cover plate 509 is pressed against the rotating plate 511 hinged and matched through torsion springs in the hinge seat 510 again, the tilting action of the rotating plate 511 is caused, the one-to-one correspondence effect between the plurality of protruding blocks 513 and the plurality of grooves 514 is realized, and when the one-to-one correspondence between the plurality of protruding blocks 513 and the plurality of grooves 514 is finished, the pressing of the rotating plate 511 hinged and matched through torsion springs in the hinge seat 510 is stopped, so that the rotating plate 511 is enabled to be pressed down under the reset action of the torsion springs, the tops of the test tubes 601 fixed at the positions are sealed and fixed one by one, and liquid to be detected from the tops of the test tubes 601 is prevented from being uniformly sprayed in the process of shaking and sampling in the later period;
When the whole device is subjected to shaking sampling treatment before liquid to be detected is detected, the driving motor 517 is synchronously started to drive the eccentric wheel 518 fixed on the output shaft of the driving motor 517 to rotate, so that the rotating groove 519 formed at the top of the eccentric wheel 518 is driven to slide on the peripheral side surface of the sliding rod 520, a reciprocating acting force is provided for the sliding rod 520, and the movable bottom plate 505 is driven to horizontally move in a reciprocating manner between the two limiting grooves 504, so that the liquid to be detected in the test tube 601 is further subjected to further shaking reciprocating operation in the horizontal direction, the mixing degree of the liquid to be detected in the test tube 601 is further increased, and the influence on the later sampling detection process is avoided;
When the whole device is subjected to shaking sampling before liquid to be detected is detected, the servo motor 305 is started to drive the reciprocating arm 306 fixed on the output shaft of the servo motor 305 to rotate, so that the reciprocating rod 307 fixed on the top of the reciprocating arm 306 slides repeatedly in the reciprocating groove 410, the reciprocating movement process of the U-shaped bottom plate 401 in the support frame 301 is realized, the inclined lifting process before shaking sampling is facilitated, the liquid to be detected in the test tubes 601 is subjected to shaking operation preliminarily, and errors caused in the later sampling detection process are avoided.
Referring to fig. 1-16, the third embodiment is an improvement on the basis of the first embodiment, specifically, an inwardly extending sample outlet tube 603 is communicated with the bottom of a test tube 601, a sliding rod 604 is slidably arranged in the sample outlet tube 603, a rubber sealing pad 605 is fixed on the top of the sliding rod 604 in the test tube 601, an annular groove 606 which is in clamping fit with the top of the sample outlet tube 603 is fixed on the bottom of the rubber sealing pad 605, and the diameter of the sliding rod 604 is smaller than the inner diameter of the sample outlet tube 603; a positioning circular plate 607 is fixed at the bottom of the sliding rod 604, and an elastic spring 608 is fixed between the positioning circular plate 607 and the sampling tube 603; a plurality of guide rods 522 are fixed at the bottom of the movable bottom plate 505; the sampling piece 7 comprises a sampling plate 701, a plurality of sampling cylinders 702 are communicated with the bottom of the sampling plate 701, a collision rod 703 is fixed at the bottom of the sampling cylinder 702, and a plurality of guide holes 704 which are in sliding fit on the guide rods 522 are formed in the top of the sampling plate 701 in a penetrating manner; two symmetrical supporting plates 523 are fixed at the bottom of the movable bottom plate 505, a telescopic cylinder 524 is fixed on one side of the supporting plate 523, a positioning cylinder 525 is fixed at the telescopic end of the telescopic cylinder 524, a T-shaped plate 526 is fixed on one side of the positioning cylinder 525, a rotary column 527 is fixed on one side of the T-shaped plate 526, a lifting arm 528 is in rotary fit with the peripheral side of the rotary column 527, a push-pull rod 529 is fixed on one side of the lifting arm 528, and an inclined panel 530 mutually attached to the lifting arm 528 is fixed below the rotary column 527 on one side of the T-shaped plate 526; the lug plates 705 are fixed on the opposite side surfaces of the sample plate 701, the side plates 706 are fixed on the bottom of the lug plates 705, and the displacement grooves 707 in sliding fit with the push-pull rods 529 are formed in one side of the side plates 706.
The specific application of the third embodiment is as follows:
After shaking up before detection, the device starts to synchronously start the two telescopic cylinders 524 to drive the positioning cylinders 525 fixed at the telescopic ends of the two telescopic cylinders 524 to move, so that the push-pull rod 529 fixed at one side of the lifting arm 528 gradually approaches the inner wall of the displacement groove 707 until the peripheral side surface of the push-pull rod 529 and the inner wall of the displacement groove 707 are in a complete state, and continues to start the two telescopic cylinders 524 to continue to extend, at this time, because the peripheral side surface of the push-pull rod 529 and the inner wall of the displacement groove 707 are in a complete state, and the lifting arm 528 is rotationally connected to the peripheral side surface of the rotating column 527 fixed at one side of the T-shaped plate 526, when the two telescopic cylinders 524 continue to extend, the lifting arm 528 starts to rotate in an upward lifting manner at the peripheral side surface of the rotating column 527, and provides an upward moving force for the sample plate 701 slidingly matched with the circumferential sides of the guide rods 522, along with the upward moving process of the sample plate 701 on the circumferential sides of the guide rods 522, the abutting rod 703 fixed at the bottom of the sampling tube 702 starts to gradually approach the bottom of the positioning circular plate 607 and simultaneously gives an upward moving force to the positioning circular plate 607, so as to compress the elastic spring 608 fixedly connected between the positioning circular plate 607 and the sample outlet tube 603, drive the sliding rod 604 to move upward inside the sample outlet tube 603, enable the annular groove 606 arranged at the bottom of the rubber sealing gasket 605 to gradually separate from the top of the sample outlet tube 603, enable the liquid to be detected after shaking inside the test tube 601 to flow out from the inside of the sample outlet tube 603 and finally fall into the inside of the sampling tube 702, thereby performing a shaking sampling process before detection, so as to facilitate the flow type fluorescence detection of the liquid to be detected after shaking and sampling by a detector;
After the above actions are completed, starting to synchronously start the two telescopic cylinders 524 again, driving the positioning cylinders 525 fixed by the telescopic ends of the two telescopic cylinders 524 to reversely move, so that the push-pull rod 529 fixed by one side of the lifting arm 528 is gradually far away from the inner wall of the displacement groove 707 until the circumference side of the push-pull rod 529 is separated from the displacement groove 707, closing the two telescopic cylinders 524, simultaneously manually supporting the two ear plates 705 of the sample plate 701, then applying a downward sliding acting force to the two ear plates 705, driving the whole sample piece 7 to move downwards on the circumference sides of the guide rods 522 and finally separating from the guide rods 522, and placing the whole sample piece 7 inside the flow type fluorescence detector (the flow type fluorescence detector is not drawn in the drawing), and the working detection process of the flow type fluorescence detector is the prior art, and particularly can refer to two Chinese invention patents with publication number of CN105388139A, CN114414538A, so that the concrete detection process of the flow type fluorescence detector is not excessively described here, the whole detection process is simplified by the flow type fluorescence detector, the whole detection process can be carried out by the flow type fluorescence detector, the sample structure is not uniformly and the sample liquid can be uniformly detected after the whole detection process is carried out, and the sample structure is uniformly, and the sample liquid can be sampled after the sample is uniformly detected, the sample structure is not required to be uniformly;
Before taking the whole sample piece 7, through the mutual laminating effect between inclined panel 530 and the lifting arm 528, the lifting arm 528 which is rotationally connected to the circumference side of the rotary column 527 is supported, so that the sample piece 7 which is in sliding fit between a plurality of guide rods 522 is supported, the phenomenon that the sample piece 7 falls off when shaking up before detection is prevented, the influence on the later sampling detection process is avoided, the later opening end of the top of the test tube 601 is sealed in the process of taking the whole sample piece 7, along with the continuous reverse movement process of two telescopic cylinders 524, the push-pull rod 529 and the displacement groove 707 are driven to gradually separate, the interference rod 703 fixed at the bottom in the sampling cylinder 702 begins to gradually separate from the bottom of the positioning circular plate 607 in the process of beginning separation of the push-pull rod 529 and the displacement groove, at this time, the compressed elastic spring 608 begins to elastically reset, the sliding rod 604 is driven to move downwards in the inside of the sample tube 603, the annular groove 606 formed at the bottom of the rubber sealing pad 605 gradually approaches to the top of the sample tube 603, so that the top opening end of the test tube 601 is sealed, the later liquid is prevented from polluting the inside of the test tube 601 after the detection process of the inside of the sample tube 601, and the inside of the test tube 601 is prevented from being polluted by the shaking up.
It will be apparent that the embodiments described above are merely some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present application and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that embodiments of the application described herein may be implemented in sequences other than those illustrated or otherwise described herein.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above examples, and all technical solutions belonging to the concept of the present invention belong to the protection scope of the present invention. It should be noted that modifications and adaptations to the present invention may occur to one skilled in the art without departing from the principles of the present invention and are intended to be within the scope of the present invention.

Claims (8)

1. Flow fluorescence detection pretreatment equipment, including shaking even subassembly (1), its characterized in that: the sample assembly (2) is in sliding fit with the shaking-up assembly (1);
The shaking-up assembly (1) comprises a bracket piece (3) and a reciprocating piece (4) which is in sliding fit with the bracket piece, the sample assembly (2) comprises a positioning piece (5) which is in hinged fit with the reciprocating piece (4), a plurality of test tube pieces (6) which are inserted and fixed in the positioning piece (5) and sampling pieces (7) which are in sliding fit with the bottom of the positioning piece (5);
The support component (3) comprises a support frame (301), wherein oblique grooves (302) are formed in the opposite outer side surfaces of the support frame (301) in a penetrating mode, two symmetrical vertical plates (303) are fixed on the opposite outer side surfaces of the support frame (301), and U-shaped guide plates (304) are fixed on one side of each vertical plate (303) above the support frame (301);
the reciprocating piece (4) comprises a U-shaped bottom plate (401) which is in sliding fit between two U-shaped guide plates (304), first pin shafts (402) are respectively fixed on two opposite inner side walls of the U-shaped bottom plate (401), first hinge arms (403) are hinged and matched on the peripheral side faces of the first pin shafts (402), second pin shafts (404) are respectively fixed on two opposite inner side walls of the U-shaped bottom plate (401), second hinge arms (405) are hinged and matched on the peripheral side faces of the second pin shafts (404), and first hinge holes (406) and second hinge holes (407) are respectively formed in one side of each of the first hinge arms (403) and one side of each of the second hinge arms (405) in a penetrating mode;
The locating piece (5) comprises a U-shaped sliding plate (501), a third pin shaft (502) in hinged fit with the first hinge hole (406) is fixed on two opposite outer sides of the U-shaped sliding plate (501), a fourth pin shaft (503) in hinged fit with the second hinge hole (407) is fixed on two opposite outer sides of the U-shaped sliding plate (501), and the third pin shaft (502) is in sliding fit with the inclined groove (302).
2. A flow-type fluorescence detection pretreatment device according to claim 1, wherein: a servo motor (305) is fixed at the outer bottom of the supporting frame (301), a reciprocating arm (306) is fixed in the supporting frame (301) at the output shaft of the servo motor (305), and a reciprocating rod (307) is fixed at the top of the reciprocating arm (306);
a vertical plate (408) is fixed on the outer side surface of the U-shaped bottom plate (401), a reciprocating plate (409) is fixed at the bottom of the vertical plate (408), and a reciprocating groove (410) which is in sliding fit with the peripheral side surface of the reciprocating rod (307) is formed in the top of the reciprocating plate (409) in a penetrating mode.
3. A flow-type fluorescence detection pretreatment device according to claim 2, wherein: limiting grooves (504) are formed in two opposite inner side surfaces of the U-shaped sliding plate (501), a movable bottom plate (505) is slidably matched between the two limiting grooves (504), symmetrical two side baffles (506) are fixed at the top of the movable bottom plate (505), a top sealing plate (507) is hinged between the two side baffles (506), and a handle rod (508) is fixed at the top of the top sealing plate (507);
And a sealing rubber cover plate (509) is fixed at the bottom of the top sealing plate (507).
4. A flow-type fluorescence detection pretreatment device according to claim 3, wherein: a hinging seat (510) is fixed at the center of one side of the top sealing plate (507), a rotating plate (511) is hinged and matched inside the hinging seat (510) through a torsion spring, a positioning plate (512) is fixed on one side of the rotating plate (511), and a plurality of protruding blocks (513) are fixed on one side of the positioning plate (512);
A plurality of grooves (514) matched with the protruding blocks (513) are formed in one side of the movable bottom plate (505).
5. The flow-type fluorescence detection pretreatment device of claim 4, wherein: two symmetrical connecting plates (515) are fixed at the bottom of the U-shaped sliding plate (501), a mounting bottom plate (516) is jointly fixed at the bottoms of the two connecting plates (515), a driving motor (517) is fixed at the top of the mounting bottom plate (516), an eccentric wheel (518) is fixed on an output shaft of the driving motor (517), and a rotating groove (519) is formed in the top of the eccentric wheel (518);
a sliding rod (520) which is in sliding fit with the inside of the rotating groove (519) is fixed at the bottom of the movable bottom plate (505);
the top of the movable bottom plate (505) is communicated with a plurality of sleeves (521);
The test tube (6) comprises a test tube (601) which is inserted and matched in the sleeve (521), and a positioning ring (602) which is mutually attached to the top of the movable bottom plate (505) is fixed on the peripheral side surface of the test tube (601).
6. The flow-type fluorescence detection pretreatment device of claim 5, wherein: the bottom of the test tube (601) is communicated with an inwardly extending sampling tube (603), a sliding rod (604) is arranged in the sampling tube (603) in a sliding mode, a rubber sealing gasket (605) is fixed in the test tube (601) at the top of the sliding rod (604), an annular groove (606) which is matched with the top of the sampling tube (603) in a clamping mode is fixed at the bottom of the rubber sealing gasket (605), and the diameter of the sliding rod (604) is smaller than the inner diameter of the sampling tube (603);
A positioning circular plate (607) is fixed at the bottom of the sliding rod (604), and an elastic spring (608) is fixed between the positioning circular plate (607) and the sampling tube (603).
7. The flow-type fluorescence detection pretreatment device of claim 6, wherein: a plurality of guide rods (522) are fixed at the bottom of the movable bottom plate (505);
The sampling piece (7) comprises a sample discharging plate (701), a plurality of sampling cylinders (702) are arranged at the bottom of the sample discharging plate (701) in a communicating mode, a contact supporting rod (703) is fixed at the inner bottom of each sampling cylinder (702), and a plurality of guide holes (704) which are in sliding fit on the guide rods (522) are formed in the top of the sample discharging plate (701) in a penetrating mode.
8. The flow-type fluorescence detection pretreatment device of claim 7, wherein: two symmetrical supporting plates (523) are fixed at the bottom of the movable bottom plate (505), a telescopic cylinder (524) is fixed on one side of the supporting plates (523), a positioning cylinder (525) is fixed at the telescopic end of the telescopic cylinder (524), a T-shaped plate (526) is fixed on one side of the positioning cylinder (525), a rotating column (527) is fixed on one side of the T-shaped plate (526), a lifting arm (528) is rotationally matched with the peripheral side of the rotating column (527), a push-pull rod (529) is fixed on one side of the lifting arm (528), and an inclined plate (530) mutually attached to the lifting arm (528) is fixed below the rotating column (527) on one side of the T-shaped plate (526).
The lug plates (705) are fixed on two opposite side surfaces of the sample plate (701), side plates (706) are fixed at the bottoms of the lug plates (705), and displacement grooves (707) in sliding fit with the push-pull rods (529) are formed in one side of each side plate (706).
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