CN118806871A - Composition with alcohol sobering and hangover-resistant effects, preparation method and application - Google Patents

Abstract

The invention discloses a composition with alcohol sobering and anti-hangover effects, a preparation method and application, and belongs to the technical field of medicines or health foods. The composition comprises the following raw materials in parts by weight: 100-125 parts of glutathione and 10-20 parts of nervonic acid. The invention uses glutathione and nervonic acid as raw materials to prepare a composition with alcohol sobering and anti-hangover effects, reduces the drunkenness rate, shortens the drunkenness time, and effectively alleviates the dizziness and headache symptoms after drunkenness.

Description

Composition with alcohol sobering and hangover-resistant effects, preparation method and application

Technical Field

The invention relates to the technical field of medicine or health food processing, in particular to a composition with alcohol sobering and anti-hangover effects, and a preparation method and application thereof.

Background Art

The metabolic process of alcohol in the human body includes alcohol absorption and alcohol metabolism. For alcohol absorption, alcohol is ethanol. Its molecular structure determines that it is fat-soluble and water-soluble. It can pass through the cell membrane and blood-brain barrier very quickly, so the absorption speed is extremely fast. After alcohol enters the mouth, it is only absorbed in small amounts by the mucous membrane in the mouth, and a large amount enters the stomach. In the stomach, about 10-20% of the ethanol is absorbed and then enters the intestine. The intestine is the main site for ethanol absorption. Based on the lipophilicity of ethanol, it is quickly absorbed into the blood through the intestinal villi cells, and 80% is absorbed by the small intestine. The intestine cannot prevent this absorption behavior. Finally, it enters the liver and reaches the main site of ethanol metabolism. In the same ethanol metabolism process, the heart pumps a lot of blood into the brain, and the brain lipids have a very good absorption capacity for alcohol. Therefore, before reaching equilibrium, the alcohol concentration in the brain is higher than that in the blood, and the brain becomes the fastest and most significantly affected area. This is also the scientific basis for many manifestations such as "alcohol rushing straight to the forehead" when starting to drink and headaches after being drunk. Regarding alcohol metabolism, only 2-10% of the alcohol that enters the body is excreted through urine, sweat, and exhalation. The remaining 90-98% enters the liver through the blood, undergoes multiple metabolisms, becomes water and carbon dioxide, and is finally excreted from the body through the human circulation.

Prior art CN202010279885.9, published on June 19, 2020, discloses a hangover and liver protection extract, composition, preparation method and application. The extract is prepared from the following ingredients by mass: 200-300 parts of Ganoderma lucidum, 150-250 parts of Wanniangan, and 150-250 parts of black plum. The composition includes hangover and liver protection extract, glutathione, vitamin C and lactoferrin, which has the effect of reducing the alcohol content in the breath within 4 hours of drinking and protecting the liver, but does not have the function of improving the symptoms of dizziness and headache after drunkenness, and cannot meet people's demand for sobering consumption.

On this basis, the present application creates a composition, preparation method and application with anti-hangover effect that can reduce the drunkenness rate, shorten the drunkenness time, and relieve headache and dizziness symptoms, so as to overcome the shortcomings of the existing alcohol detoxification composition.

Summary of the invention

The technical problem to be solved by the present invention is to provide a composition with alcohol sobering and anti-hangover effects, which can reduce the drunkenness rate, shorten the drunkenness time, and relieve headache and dizziness symptoms, as well as a preparation method and application.

In order to solve the above technical problems, the present invention provides a composition with alcohol sobering and anti-hangover effects, comprising the following raw materials in parts by weight: 100-125 parts of glutathione and 10-20 parts of neuraminic acid.

Glutathione is a tripeptide containing γ-amide bonds and sulfhydryl groups, which is composed of glutamic acid, cysteine and glycine. Glutathione helps maintain normal immune system function, and has antioxidant and integrated detoxification effects. It plays an important role in a variety of cellular biochemical processes, such as free radical neutralization, detoxification, cysteine transportation and storage, maintenance of cellular redox, and regeneration of ascorbic acid and vitamin E. Glutathione can activate the activity of alcohol dehydrogenase, eliminate the toxic effects of ethanol on the liver, and can also remove hydroxyl free radicals in the human body, protecting the sulfhydryl groups in many proteins and enzymes from being oxidized by acetic acid and acetaldehyde produced by ethanol metabolism, thereby ensuring that proteins and enzymes can perform their physiological functions normally.

Neuroacid is the core natural component of brain nerve cells and nerve tissues. It is a special substance that promotes the repair and regeneration of damaged nerve cells and tissues. Neuroacid is a drug that regulates mental nutrition metabolism. It can repair damaged nerves in the human brain, promote normal development of the brain, and enhance the brain's reaction ability and memory. Neuroacid mainly acts on nerves and can repair nerve fibers. In the process of repairing nerve fibers, it will stimulate the repair cells to ensure that the nerve pathways are unobstructed. Even if there is no disease, taking neuroacid can prevent nerve damage.

The researchers of the present invention unexpectedly discovered during the study that taking a small amount of neuraminic acid and glutathione simultaneously can significantly reduce the drunkenness rate and shorten the drunkenness time, and relieve the symptoms of dizziness and headache after drunkenness.

The composition composed of nervonic acid and glutathione has the above functions, and the mechanism is as follows: first, nervonic acid can enhance the activity of glutathione peroxidase, which is a very powerful reductase and one of the barriers against body peroxidation. The liver is an important organ for the production of oxidative stress. During the metabolism of ethanol, especially when ethanol is excessive, a large number of free radicals will be produced, which will damage liver cells. Increasing the activity of glutathione peroxidase has a certain effect on protecting the liver and preventing the liver from producing excessive oxygen free radicals, sobering up and protecting the liver, and can improve liver function. Glutathione peroxidase can catalyze reduced glutathione into oxidized glutathione, reducing toxic peroxides to non-toxic hydroxyl compounds, thereby protecting the structure and function of liver cells from interference and damage by peroxides.

Secondly, alcohol is metabolized into acetaldehyde by alcohol dehydrogenase in the liver. Some acetaldehyde enters the brain through the blood circulation and is metabolized into acetic acid by acetaldehyde dehydrogenase. A large number of hydroxyl free radicals are produced in the process of metabolism. Hydroxyl free radicals are highly active and can easily cause oxidative stress. The brain is a highly oxidatively active tissue rich in lipids and proteins that are susceptible to oxidation. When oxidative stress occurs in the brain, it can cause oxidative damage to neuronal membranes and organelles, destroying the structure and function of brain cells. Neuroacid can effectively cross the blood-brain barrier and has strong antioxidant activity, which can protect brain cells and reduce the oxidative stress of hydroxyl free radicals, thereby accelerating sobering up and significantly shortening the time of drunkenness.

Again, neuraminic acid eliminates the central nervous system excitement caused by alcohol, has a sedative effect, and relieves the symptoms of headache and dizziness after drinking.

Glutathione sources include but are not limited to yeast extract, animal offal, asparagus, avocado, walnuts, etc.

Neural acid is derived from Acer truncatum seed oil.

As a further improvement, the composition having the effect of sobering up and resisting hangover also includes the following raw materials in parts by weight: 10-20 parts of glycerol phosphatidylcholine.

Sources of glycerophosphatidylcholine include, but are not limited to, egg yolk and soybeans.

Glycerylphosphatidylcholine GPC is a water-soluble phospholipid metabolite naturally present in the human body, and is also an important neurotransmitter and phospholipid precursor. Glycerylphosphatidylcholine can not only effectively protect the liver and prevent fatty liver and alcoholic liver caused by alcohol metabolism. Glycerylphosphatidylcholine can also effectively cross the blood-brain barrier and enter brain cells. At the same time, glycerylphosphatidylcholine can also play an antioxidant role, combining with hydroxyl free radicals in the brain to protect brain cells, speed up sobering up, and shorten the time of drunkenness. In addition, glycerylphosphatidylcholine and neuraminic acid can cooperate to protect brain cells and avoid oxidative damage to neuronal membranes and organelles during ethanol metabolism.

As a further improvement, the composition having the effect of sobering up and resisting hangover further comprises the following raw materials in parts by weight: 50-60 parts of curcumin, 10-15 parts of dihydroquercetin, and 100-150 parts of γ-cyclodextrin.

Sources of dihydroquercetin include, but are not limited to, larch, Douglas fir, and the like.

Sources of curcumin include but are not limited to turmeric, garlic, onion, pepper, carrot, etc.

Curcumin, also known as diferuloylmethane, is the main natural polyphenol found in turmeric rhizomes. It has multiple functions such as inhibiting inflammatory response in the human body, scavenging free radicals in the human body, anti-oxidation, and anti-rheumatoid. In the prior art, curcumin is often used as a raw material for alcohol-detoxifying and liver-protecting preparations. Curcumin mainly protects the liver and stomach of the human body and enhances the liver's alcohol-detoxifying and detoxifying functions, thereby playing a certain role in detoxifying alcohol. Moreover, curcumin can enhance the detoxification function of the human liver to a certain extent, thereby reducing the damage of alcohol to various organs of the human body.

Dihydroquercetin, also known as taxifolin, taxol, quercetin, dihydroquercetin or (2R, 3R)-dihydroquercetin, is a dihydroflavonol compound belonging to the vitamin P family. It is a widely used bioactive agent. Like other flavonoids, it has a variety of biological activities in the human body, including antioxidant, free radical scavenging, antiviral, anti-inflammatory, vasodilation and antibacterial effects. Dihydroquercetin has a strong antioxidant effect and can inhibit the oxidative stress cascade. It can also reduce the levels of alanine aminotransferase and aspartate aminotransferase in serum, improve lipid deposition in the liver, and improve alcoholic fatty liver. The high oxidative activity of dihydroquercetin is also often used as a food or pharmaceutical antioxidant.

γ-cyclodextrin, in combination with glutathione, neuraminic acid, and glycerophosphatidylcholine, is used as the wall material of microcapsule compounds for encapsulating curcumin and dihydroquercetin, which can significantly improve the bioavailability of curcumin and dihydroquercetin, and further enhance the alcohol-detoxifying and anti-hangover effects of curcumin and dihydroquercetin, ultimately achieving a better effect of reducing the drunkenness rate, shortening the drunkenness time, and relieving dizziness and headache symptoms.

As a further improvement, the composition having the effect of sobering up and resisting hangover also includes the following raw materials in parts by weight: 50-60 parts of fructose.

Fructose can speed up the rate of alcohol decomposition and metabolism. The metabolism of alcohol needs to be detoxified by the liver and then excreted by the kidneys. This process requires energy participation. Adding a small amount of fructose to the composition with alcohol sobering and anti-hangover effects can speed up the energy supply rate. The main metabolism of fructose also takes place in the liver. Fructose is mainly metabolized by fructokinase in the liver and converted into glucose and lactic acid. Fructose can increase the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver, and the glucose generated by its metabolism provides energy for ethanol metabolism. Adding fructose to the composition with alcohol sobering and anti-hangover effects in this application can relieve dizziness and headaches and shorten the time of drunkenness.

As a further improvement, the composition with alcohol sobering and anti-hangover effects preferably comprises the following raw materials in parts by weight: 125 parts of glutathione, 60 parts of curcumin, 15 parts of dihydroquercetin, 20 parts of nervonic acid, 20 parts of glycerophosphatidylcholine, 145 parts of γ-cyclodextrin and 56 parts of fructose.

Magnesium stearate is used as a glidant to facilitate tableting of the composition.

As a further improvement, the present invention also provides a method for preparing a composition having the effect of sobering up and resisting hangover, comprising the following steps: mixing 10-20 parts by weight of nervonic acid, 0-20 parts by weight of glycerophosphatidylcholine and 100-125 parts by weight of glutathione; then adding 100-150 parts by weight of a filler and mixing evenly, using water as a wetting agent to make a soft material, 20 mesh sieve to make wet granules, drying, granulating, tableting, and obtaining a composition having the effect of sobering up and resisting hangover. The filler is one or more of starch, lactose, dextrin, sucrose, pregelatinized starch, microcrystalline cellulose, and inorganic salts. Preferably, the filler is γ-cyclodextrin.

As a further improvement, the present invention also provides a method for preparing a composition having alcohol sobering and anti-hangover effects, comprising the following steps:

(1) 10-20 parts by weight of nervonic acid, 10-20 parts by weight of glycerophosphatidylcholine, 10-20 parts by weight of γ-cyclodextrin and 100-125 parts by weight of glutathione are mixed, 300 parts by weight of water are added, dissolved and mixed evenly, and stirred for 2 hours to obtain a wall material coating liquid;

(2) mixing 10-15 parts by weight of dihydroquercetin and 50-60 parts by weight of curcumin, adding the mixture to the wall material coating solution obtained in step (1), mixing the mixture evenly, and freeze-drying the mixture after processing with a microcapsule granulator to obtain a microencapsulated composition;

(3) Add 80-140 parts by weight of γ-cyclodextrin to the microencapsulated composition obtained in step (2) and mix well, use water as a wetting agent to make a soft material, sieve with 20 mesh to make wet granules, dry, granulate, and tablet, to obtain a composition with alcohol sobering and anti-hangover effects.

In step (1), ultrasonic stirring is used for 2 hours, the power of ultrasonic stirring is 60-100 W, and the temperature is 55-60° C. In step (2), the vibration frequency of the microcapsule granulator is 1000 Hz, the electrode voltage is 500 V, the air pressure is 200 mbar, the nozzle diameter is 400 μm, and the flow rate is 8 mL/min.

Curcumin is an orange-yellow crystalline powder, slightly bitter, insoluble in water, poor in stability, and low in bioavailability. Both nervonic acid and glycerophosphatidylcholine have good fat solubility, and glutathione has good water solubility. The present invention uses curcumin and dihydroquercetin as core materials, and encapsulates them in microcapsules with nervonic acid, glycerophosphatidylcholine, and a portion of γ-cyclodextrin and glutathione mixed as wall materials to prepare a microencapsulated composition. The preparation method solves the problem that curcumin is insoluble in water, and greatly improves the bioavailability of curcumin and dihydroquercetin. After microencapsulation, curcumin and dihydroquercetin have good stability, high bioavailability, and good taste. That is, the γ-cyclodextrin in the preparation method not only plays the role of a filler, but also plays the role of a wall material component to achieve microencapsulation.

As a further improvement, in step (3), after the granules are granulated, 56 parts by weight of fructose and 4 parts by weight of magnesium stearate are added, mixed evenly, and then tableted to obtain a composition with alcohol sobering and anti-hangover effects.

In addition to being used in combination with neuraminic acid to have the effects of detoxifying alcohol and protecting the liver, the glutathione of the present invention is also used in combination with neuraminic acid, glycerophosphatidylcholine and γ-cyclodextrin as a microcapsule compound wall material, and can also improve the bioavailability of curcumin and dihydroquercetin, ultimately achieving the effect of the composition having the effects of reducing the drunkenness rate, shortening the drunkenness time, and relieving the symptoms of dizziness and headache.

The microencapsulated composition is added with the remaining gamma-cyclodextrin and mixed to form granules, and finally fructose and magnesium stearate are added to form tablets to obtain a composition with alcohol sobering and anti-hangover effects.

As another improvement of the present invention, the present invention also provides a use of the above-mentioned composition with alcohol sobering and anti-hangover effects in the preparation of alcohol sobering and anti-hangover medicines or health foods.

After adopting such a design, the present invention has the following advantages:

In the composition with alcohol sobering and anti-hangover effects of the present invention, neuraminic acid and glutathione cooperate to have good alcohol sobering and liver protection effects. In addition, by adding glycerol phosphatidylcholine, neuraminic acid and glycerol phosphatidylcholine can effectively pass through the blood-brain barrier, protect the brain, avoid oxidative stress, and synergistically play the role of accelerating sobering up, shortening the drunken time, and relieving the dizziness and headache caused by drunkenness. In addition, curcumin and dihydroquercetin are used as core materials, and γ-cyclodextrin, glutathione, neuraminic acid, and glycerol phosphatidylcholine are used as microcapsule compound wall materials to form a microencapsulated composition, thereby improving the stability of curcumin, greatly improving the bioavailability of curcumin and dihydroquercetin, enhancing the alcohol sobering and detoxifying function of the liver, and improving the alcohol sobering and anti-hangover effects.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is the effect of the drug in Experimental Example 1 on the drunken behavior of mice.

FIG. 2 is the test result of ethanol content in the blood of mice after multiple administrations in Experimental Example 2.

FIG. 3 is the detection result of acetaldehyde content in the blood of mice after multiple administrations in Experimental Example 2.

Figure 4 is the results of the test for the content of alanine aminotransferase in the blood of mice after multiple administrations in Experimental Example 2

FIG. 5 is the result of the detection of the content of aspartate aminotransferase in the blood of mice after multiple administrations in Experimental Example 2.

FIG. 6 is the result of the detection of the alcohol dehydrogenase content in the liver of mice after multiple administrations in Experimental Example 3.

FIG. 7 is the result of detecting the content of acetaldehyde dehydrogenase in the liver of mice after multiple administrations in Experimental Example 3.

FIG8 is the detection results of acetaldehyde content, 6-OH-MTHβC content and AchE content in the brain tissue of mice after multiple administration in Experimental Example 4.

FIG. 9 is the HE staining result of paraffin sections of the liver and stomach of mice after multiple administrations in Experimental Example 5.

FIG. 10 shows the bioavailability results of dihydroquercetin and curcumin in mice.

DETAILED DESCRIPTION

The qualified dihydroquercetin, curcumin, glutathione, γ-cyclodextrin, fructose and magnesium stearate were respectively passed through an 80-mesh sieve and set aside.

Example 1

(1) mixing 20 parts by weight of nervonic acid and 125 parts by weight of glutathione;

(2) 100 parts by weight of γ-cyclodextrin is added and mixed evenly, water is used as a wetting agent to prepare a soft material, a 20-mesh sieve is used to prepare wet granules, the granules are dried, sized, and tableted to obtain a composition having the effect of sobering up and resisting hangover.

The composition with the effect of sobering up and preventing hangover prepared in this example is a white tablet with a weight of 0.40 g. Usage: Orally, take one tablet before and after drinking.

Example 2

(1) mixing 10 parts by weight of nervonic acid, 10 parts by weight of glycerophosphatidylcholine and 100 parts by weight of glutathione;

(2) 150 parts by weight of γ-cyclodextrin is added and mixed evenly, water is used as a wetting agent to prepare a soft material, a 20-mesh sieve is used to prepare wet granules, the granules are dried, sized, and tableted to obtain a composition having the effect of sobering up and resisting hangover.

The composition with the effect of sobering up and preventing hangover prepared in this example is a white tablet with a weight of 0.40 g. Usage: Orally, take one tablet before and after drinking.

Example 3

(1) mixing 15 parts by weight of nervonic acid, 20 parts by weight of glycerophosphatidylcholine and 125 parts by weight of glutathione;

(2) 145 parts by weight of γ-cyclodextrin was added and mixed evenly, water was used as a wetting agent to prepare a soft material, a 20-mesh sieve was used to prepare wet granules, the granules were dried, sized, and tableted to obtain a composition having the effect of sobering up and preventing hangover.

The composition with the effect of sobering up and preventing hangover prepared in this example is a white tablet with a weight of 0.40 g. Usage: Orally, take one tablet before and after drinking.

Example 4

20 parts by weight of nervonic acid, 20 parts by weight of glycerophosphatidylcholine, 145 parts by weight of γ-cyclodextrin, 115 parts by weight of glutathione, 15 parts by weight of dihydroquercetin and 60 parts by weight of curcumin are mixed, water is used as a wetting agent to prepare a soft material, a 20-mesh sieve is used to prepare wet granules, the granules are dried and sized, 56 parts by weight of fructose and 4 parts by weight of magnesium stearate are added, the mixture is evenly mixed and then tabletted to obtain a composition with alcohol sobering and anti-hangover effects.

The composition with the effect of sobering up and preventing hangover prepared in this example is a light yellow tablet with a tablet weight of 0.40 g. Usage: Oral administration, one tablet before and after drinking.

Example 5

(1) adding 10 parts by weight of nervonic acid, 10 parts by weight of glycerophosphatidylcholine, 20 parts by weight of γ-cyclodextrin and 100 parts by weight of glutathione to 300 parts by weight of water to dissolve and mix evenly, and stirring the mixture under ultrasonic conditions of 60 W and 55-60° C. for 2 h to obtain a wall material coating solution;

(2) mixing 15 parts by weight of dihydroquercetin and 55 parts by weight of curcumin, adding the mixture to the wall material coating solution obtained in step (1), mixing the mixture evenly, and subjecting the mixture to a microencapsulation granulator and freeze-drying the mixture to obtain a microencapsulated composition, wherein the microencapsulation granulator has a vibration frequency of 1000 Hz, an electrode voltage of 500 V, an air pressure of 200 mbar, a nozzle diameter of 400 μm, and a flow rate of 8 mL/min;

(3) Add 100 parts by weight of γ-cyclodextrin to the microencapsulated composition obtained in step (2) and mix evenly, use water as a wetting agent to make a soft material, sieve wet granules with a 20-mesh sieve, dry, and granulate, add 55 parts by weight of fructose and 5 parts by weight of magnesium stearate, mix evenly, and then compress into tablets to obtain a composition with alcohol sobering and anti-hangover effects.

The composition with the effect of sobering up and preventing hangover prepared in this example is a light yellow tablet with a tablet weight of 0.40 g. Usage: Oral administration, one tablet before and after drinking.

Example 6

(1) adding 20 parts by weight of nervonic acid, 10 parts by weight of glycerophosphatidylcholine, 20 parts by weight of γ-cyclodextrin and 110 parts by weight of glutathione to 300 parts by weight of water, dissolving and mixing evenly, and stirring by ultrasonication at a power of 60 W and a temperature of 55-60° C. for 2 h to obtain a wall material coating liquid;

(2) mixing 10 parts by weight of dihydroquercetin and 60 parts by weight of curcumin, adding the mixture to the wall material coating solution obtained in step (1), mixing the mixture evenly, and subjecting the mixture to a microencapsulation granulator and freeze-drying the mixture to obtain a microencapsulated composition, wherein the microencapsulation granulator has a vibration frequency of 1000 Hz, an electrode voltage of 500 V, an air pressure of 200 mbar, a nozzle diameter of 400 μm, and a flow rate of 8 mL/min;

(3) Add 80 parts by weight of γ-cyclodextrin to the microencapsulated composition obtained in step (2) and mix evenly, use water as a wetting agent to make a soft material, sieve the mixture through a 20-mesh screen to prepare wet granules, dry and granulate, add 60 parts by weight of fructose and 3 parts by weight of magnesium stearate, mix evenly and then compress the granules to obtain a composition having the effect of sobering up and resisting hangover.

The composition with the effect of sobering up and preventing hangover prepared in this example is a light yellow tablet with a tablet weight of 0.40 g. Usage: Oral administration, one tablet before and after drinking.

Example 7

(1) adding 15 parts by weight of nervonic acid, 10 parts by weight of glycerophosphatidylcholine, 20 parts by weight of γ-cyclodextrin and 120 parts by weight of glutathione to 300 parts by weight of water to dissolve and mix evenly, and stirring ultrasonically at a power of 100 W and a temperature of 55-60° C. for 2 h to obtain a wall material coating liquid;

(2) mixing 15 parts by weight of dihydroquercetin and 60 parts by weight of curcumin, adding the mixture to the wall material coating solution obtained in step (1), mixing the mixture evenly, and subjecting the mixture to a microencapsulation granulator and freeze-drying the mixture to obtain a microencapsulated composition, wherein the microencapsulation granulator has a vibration frequency of 1000 Hz, an electrode voltage of 500 V, an air pressure of 200 mbar, a nozzle diameter of 400 μm, and a flow rate of 8 mL/min;

(3) Add 125 parts by weight of γ-cyclodextrin to the microencapsulated composition obtained in step (2) and mix evenly, use water as a wetting agent to make a soft material, sieve the mixture through a 20-mesh screen to make wet granules, dry and granulate, add 60 parts by weight of fructose and 5 parts by weight of magnesium stearate, mix evenly and then compress the granules to obtain a composition having the effect of sobering up and resisting hangover.

The composition with the effect of sobering up and preventing hangover prepared in this example is a light yellow tablet with a tablet weight of 0.40 g. Usage: Oral administration, one tablet before and after drinking.

Example 8

(1) adding 20 parts by weight of nervonic acid, 20 parts by weight of glycerophosphatidylcholine, 20 parts by weight of γ-cyclodextrin and 125 parts by weight of glutathione to 300 parts by weight of water to dissolve and mix evenly, and stirring ultrasonically at a power of 100 W and a temperature of 55-60° C. for 2 h to obtain a wall material coating liquid;

(2) mixing 15 parts by weight of dihydroquercetin and 60 parts by weight of curcumin, adding the mixture to the wall material coating solution obtained in step (1), mixing the mixture evenly, and subjecting the mixture to a microencapsulation granulator and freeze-drying the mixture to obtain a microencapsulated composition, wherein the microencapsulation granulator has a vibration frequency of 1000 Hz, an electrode voltage of 500 V, an air pressure of 200 mbar, a nozzle diameter of 400 μm, and a flow rate of 8 mL/min;

(3) Add 125 parts by weight of γ-cyclodextrin to the microencapsulated composition obtained in step (2) and mix evenly, use water as a wetting agent to make a soft material, sieve the mixture through a 20-mesh screen to prepare wet granules, dry and granulate, add 56 parts by weight of fructose and 4 parts by weight of magnesium stearate, mix evenly and then compress the granules to obtain a composition having the effect of sobering up and resisting hangover.

The composition with the effect of sobering up and preventing hangover prepared in this example is a light yellow tablet with a tablet weight of 0.40 g. Usage: Oral administration, one tablet before and after drinking.

Experimental example: Animal experiment on drunkenness model.

Experimental groups: 240 SPF male C57 mice aged 6-8 weeks, weighing 25±2g, were randomly divided into 10 groups after 3 days of adaptive feeding, with 24 mice in each group.

They are blank control group (gavage with equal dose of normal saline for 30 days); model group (gavage with equal dose of normal saline for 30 days); experimental groups 1-8 (gavage with equal dose of the compositions obtained in Examples 1-8 for 30 days).

It should be noted that the conversion method for the oral administration dose for mice is: the daily dose for humans (60kg) is 13.3mg/kg, and the daily dose for mice should be 80mg/kg, that is, the daily oral administration dose for each mouse is 2mg; the samples in the experimental group were diluted with normal saline for oral administration.

Experimental Example 1 Experiment on the effects of alcohol on mouse behavior

Experimental detection: 1 hour after the last administration, except for the blank control group, mice in each group were gavaged with 10 ml/kg of 56-degree liquor (Beijing Erguotou) according to the mouse intoxication dose. The blank control group was gavaged with the same volume of normal saline. The gavage time point, gavage dose, righting reflex disappearance time, righting reflex recovery time of each mouse in each group were recorded, and the drunkenness rate (drunkenness rate = number of drunk mice/total number of mice in the group * 100%), drunkenness tolerance time and sobering time were calculated.

Among them, the standard for judging the drunken state is: after drinking, the mouse is placed with its back facing downward and its limbs facing upward to observe whether the righting reflex disappears. If it disappears and lasts for 30 seconds, it can be judged as a drunken state.

Criteria for determining the sober state: After the mouse enters the drunken state, observe whether the righting reflex is restored during the observation period. If the turning over time is shorter than 30s, it can be determined to be in the sober state.

The drunken behavior and data,results are shown in Table 1 and Figure 1.

Table 1 Effects of different intragastric administration drugs of equal doses on the drunken behavior of mice

Note: * Compared with the model group, there is a statistical difference (P<0.05); ** Compared with the model group, there is a significant difference (P<0.01)

It can be seen from the above table:

① Regarding the drunkenness rate results, compared with the model group, the drunkenness rates of experimental groups 1-8 were all reduced, and Examples 5-8 were all more effective than Examples 1-4. Among them, the drunkenness rate of experimental group 1 was reduced by 29.2% compared with the model group, and the drunkenness rate of experimental group 8 was reduced by 66.7% compared with the model group, which was the best effect.

② Regarding the drunkenness tolerance time and sobering time, compared with the model group, the drunkenness tolerance time of experimental groups 1-8 was prolonged, the sobering time was shortened, and the effects of Examples 5-8 were better than those of Examples 1-4. Among them, the sobering time of experimental group 1 was shortened by 10.86 minutes compared with the model group, and the sobering time of experimental group 8 was shortened by 45.67 minutes compared with the model group, which was the best effect.

From the above results, it can be concluded that the composition obtained in Example 1, which only includes glutathione and nervonic acid, can achieve a good hangover effect, significantly reduce the drunkenness rate of mice, prolong the drunkenness tolerance time, and shorten the sobering time. The compositions obtained in Examples 5-8, by adding glycerophosphatidylcholine, dihydroquercetin and curcumin, and improving the composition structure by coating process, obtain better and more effective technical effects of reducing drunkenness rate, prolonging drunkenness tolerance time and accelerating sobering time, and the effect is significant.

Experimental Example 2 Effects of Multiple Administrations on Blood Index Data of Mice

After the administration of liquor, the mice were eyeballed and blood was collected at five time points: 0.5h, 1h, 2h, 3h, and 4h. The whole blood was placed in a test tube with anticoagulant, sealed, and the ethanol and acetaldehyde content in the blood was analyzed using a gas chromatograph.

At the same time, the eyeballs were removed and blood was collected at five time points: 0.5h, 1h, 2h, 3h, and 4h. The whole blood was placed in a test tube with an anticoagulant, sealed, and the ELAS kit was used to accurately measure alanine aminotransferase ALT and aspartate aminotransferase AST. The results are shown in Table 2 and Figures 2-5. Table 2 and Figures 2-5 show the effects of different oral administrations of equal doses of drugs on the blood index data of mice.

Table 2 Effects of different intragastric administration drugs of equal doses on blood index data of mice

As can be seen from Table 2 and Figure 2, the ethanol content in the blood of mice gradually increased over time, reaching a peak at 2 hours, and then gradually decreased over time. The ethanol concentration in the blood of mice in the experimental groups 1-8 was lower than that in the model group, indicating that the composition of the present invention has a significant effect of promoting ethanol metabolism and reducing ethanol concentration. At 4 hours, the ethanol concentration in the blood of mice in the experimental groups 5-8 was significantly lower than that in the experimental groups 1-4, and the anti-hangover effect of the experimental group 8 was particularly obvious.

As can be seen from Table 2 and Figure 3, the acetaldehyde content in the blood of mice in the model group reached a peak at the 2nd hour, while the acetaldehyde content in the blood of mice in the experimental groups 1-8 reached a peak at the 1st hour, and then gradually decreased with time. This shows that the experimental groups 1-8 can promote the metabolism of most ethanol into acetaldehyde within 1 hour, while the acetaldehyde generation rate in the model group is slower, that is, the ethanol metabolism rate is slower. The anti-hangover effect of experimental group 8 is the most obvious.

As can be seen from Table 2 and Figures 4 and 5, the activities of alanine aminotransferase and aspartate aminotransferase in the blood of mice in the blank control group did not change much, while the activities of alanine aminotransferase and aspartate aminotransferase in the model group were both higher, with significant differences. This shows that the model group of this experimental example has been successfully modeled. The enzyme activities of alanine aminotransferase ALT and aspartate aminotransferase AST in the blood can directly reflect the degree of liver damage. When the activities of the two enzymes increase, the liver is severely damaged, and when the activities of the two enzymes decrease, the liver damage is weakened. The concentration of alanine aminotransferase in experimental groups 1-8 is between the blank control group and the model group. This shows that the composition obtained in experimental groups 1-8 of the present invention can reduce the concentration of alanine aminotransferase and aspartate aminotransferase in the blood, avoid liver damage caused by ethanol metabolism, have obvious liver protection effects, and the composition treated by the coating process is particularly effective.

Experimental Example 3 Effects of multiple administrations on liver index data of mice

The mice were killed at 0.5h, 2h, and 4h, respectively, and the livers were removed and homogenized, and the activities of alcohol dehydrogenase ADH and acetaldehyde dehydrogenase ALDH were measured using ELAS kits.

The enzyme activities of alcohol dehydrogenase ADH and acetaldehyde dehydrogenase ALDH in the liver can directly reflect the body's ability to metabolize ethanol and acetaldehyde. When the activities of the two enzymes increase, the alcohol metabolism ability becomes stronger, and when the activities of the two enzymes decrease, the alcohol metabolism ability becomes weaker.

Table 3 and Figures 6 and 7 show the effects of different oral gavage drugs at equal doses on the liver index data of mice.

Table 3 Effects of different oral administration drugs of equal doses on liver index data of mice

As shown in Table 3 and Figures 6 and 7, compared with the blank control group, the enzyme activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the model group were significantly increased, and the difference was significant, indicating that the model group of this experimental example has been successfully modeled. Compared with the model group, the enzyme activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver of experimental groups 1-8 at the 1-2h time point were significantly increased. Compared with the model group, the enzyme activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver of experimental groups 1-8 at the 0-2h time point showed an obvious upward trend; the enzyme activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver of experimental group 8 at the 2-4h time point were particularly obvious.

From the above results, it can be concluded that the composition of the present invention with the effect of sobering up and resisting hangover can significantly reduce the content of ethanol and acetaldehyde in the blood, and significantly reduce the enzyme activities of alanine aminotransferase and aspartate aminotransferase, and significantly increase the enzyme activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver, and the change trend is more obvious. It has significant sobering up and liver protecting functions, and the effect of the composition treated by the coating process is particularly significant.

Experimental Example 4 Effects of multiple administrations on the liver, stomach and brain tissues of mice

After the test, all mice were killed, and 8 mice from the blank control group and experimental group 1 were randomly selected for HE staining of liver and stomach sections, and the remaining 4 mice were tested for brain tissue together with other groups.

Since the acetaldehyde content in the brain directly reflects the concentration of acetaldehyde in brain tissue, acetaldehyde can interfere with the normal metabolism of neurotransmitters in the nervous system; acetaldehyde condenses with 5-hydroxytryptamine (5-HT) to form 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-β-carboline (6-OH-MTHβC). 6-OH-MTHβC is a neurotoxin. Alcohol intake can cause an increase in the content of 6-OH-MTHβC in the body, causing degenerative lesions in some neural pathways, confusion and memory loss; acetylcholinesterase (AchE). When excessive alcohol intake occurs, the acetylcholinesterase content in the body is too low, and symptoms such as dizziness, headache, and memory loss may occur.

Therefore, in this experimental example, the acetaldehyde, 6-OH-MTHβC and AchE contents in the brain tissue of experimental mice were detected 2 hours after drug administration. Table 4 and FIG. 8 show the effects of different intragastric administration of equal doses of drugs on the data of various indicators in the brain tissue of mice.

Table 4 Effects of different intragastric administration drugs of equal doses on brain tissue index data of mice (time point 2h)

Note: * Compared with the model group, there is a statistical difference (P<0.05); ** Compared with the model group, there is a significant difference (P<0.01).

From Table 4 and Figure 8, we can see that compared with the model group, the acetaldehyde and 6-OH-MTHβC contents in the brain of experimental groups 1-8 were reduced at 2h, and the AchE enzyme activity was increased; and compared with experimental groups 1-4, the acetaldehyde and 6-OH-MTHβC contents in the brain of experimental groups 5-8 were significantly reduced at 2h, and the AchE enzyme activity was significantly increased, among which the effect of experimental group 8 was particularly obvious.

From the above results, it can be concluded that the composition of the present invention reduces the concentration of acetaldehyde in the brain, reduces the damage caused by alcohol to brain cognition, and helps the body recover sobriety after drinking. This shows that the composition of the present invention can significantly relieve symptoms such as dizziness and headache after drunkenness, reduce the drunkenness rate, shorten the drunkenness time, and the effect of the composition treated by the coating process is particularly significant.

Experimental Example 5 HE staining of liver and stomach sections

HE staining is a commonly used histological staining method. It uses two dyes, hematoxylin and eosin, to stain tissues, which can clearly show cell morphology and tissue structure. Among them, hematoxylin can make the cell nucleus blue, and eosin can make the cytoplasm red, thereby locating and identifying cells. Figure 9 shows the results of HE staining of paraffin sections of the liver and stomach of mice in the model group and experimental group 8.

As shown in Figure 9: ① HE staining of liver paraffin sections showed that the model group had irregular arrangement of liver cells, tiny lipid accumulation vacuoles were visible in the liver cells of mice, and a small amount of inflammation was produced; while the liver tissue damage of mice in experimental group 8 was significantly weaker. ② HE staining of gastric paraffin sections showed that the gastric mucosa of the model group had a certain degree of damage, mucosal edema, epithelial cell loss, and thinning of the gastric mucosal layer; while the gastric mucosa of mice in experimental group 8 had only a small amount of damage.

From the above results, it can be concluded that the composition of the present application has a significant protective effect on the liver tissue and stomach tissue of the body that has ingested alcohol, reduces the damage of alcohol to the liver and stomach, and has the effect of protecting the liver and stomach.

Experimental Example 6 Bioavailability Experiment

The 24-hour excrement (feces and urine) of the mice in experimental groups 4-8 were collected on the 22nd day after continuous administration for 21 days, and the bioavailability of the product was analyzed using dihydroquercetin and curcumin as detection indicators.

The efficiency of biological resource utilization is estimated by measuring the amount of energy or nutrients consumed in biological excrement. The commonly used method is to measure the energy and substance content in urine and feces and compare them with the energy and substance content in the diet. Due to the high bioavailability of glutathione, nervonic acid and glycerophosphatidylcholine, they have no reference value. The product uses dihydroquercetin and curcumin as detection indicators to determine the bioavailability of the product. The results are shown in Table 5 and Figure 10 below.

Table 5 Effects of different oral administration of equal doses of drugs on bioavailability index data of mice

As shown in Table 5 and Figure 10, for curcumin, the bioavailability of curcumin in experimental groups 5-8 was significantly improved compared with that in experimental group 4, among which the bioavailability of curcumin in experimental group 8 was 21.12% higher than that in experimental group 4, indicating that the microencapsulation process can significantly improve the bioavailability of curcumin. For dihydroquercetin, the bioavailability of dihydroquercetin in experimental groups 5-8 was significantly improved compared with that in experimental group 4, among which the bioavailability of dihydroquercetin in experimental group 8 was 21.27% higher than that in experimental group 4. This indicates that the microencapsulation process also has a significant effect on improving the bioavailability of dihydroquercetin.

From the results of the above experimental examples, it can be concluded that the composition obtained by adding nervonic acid, glycerophosphatidylcholine and improving the preparation process of dihydroquercetin, curcumin and glutathione shows significant superiority in the body's bioavailability. At the same time, it can be seen that curcumin and dihydroquercetin are mainly absorbed and metabolized through the intestine. This result can give a reasonable explanation for the results of the above-mentioned drunk model animal test, that is, due to the improvement of the raw materials and preparation process of the composition, its bioavailability is significantly improved, thereby achieving a better reduction in drunkenness rate, prolonging drunkenness tolerance time and accelerating sobering time, reducing the damage caused by alcohol to brain cognition, assisting the body to recover sobriety after drinking, and protecting the liver and stomach. The technical effect shows that the new formula product optimized by the process of this application can use its improved bioavailability to achieve the effect of improving the effects of sobering up, resisting hangovers, protecting the liver, protecting the liver and protecting the stomach.

The composition with alcohol sobering and anti-hangover effects of the present invention can greatly improve the bioavailability of the product in the body, play the role of reducing the drunkenness rate, prolonging the drunkenness tolerance time and reducing the sobering time; at the same time, it can significantly reduce the ethanol and acetaldehyde contents in the blood and improve the enzyme activities of alcohol dehydrogenase and acetaldehyde dehydrogenase, as well as significantly reduce the acetaldehyde and 6-OH-MTHβC contents in the brain and improve the AchE enzyme activity, reflecting that the product can inhibit acetaldehyde from passing through the blood-brain barrier, reduce the damage of alcohol to brain cognition, assist the body in recovering sobriety after drinking, and achieve the effect of significantly improving the alcohol sobering and anti-hangover functions.

The composition of the present invention can also significantly reduce the enzyme activities of alanine aminotransferase and aspartate aminotransferase, and HE staining of paraffin sections shows that it can significantly protect the liver tissue and gastric tissue of the body that has ingested alcohol, indicating that the composition can not only enhance the sobering and anti-hangover effects, but also reduce the damage of alcohol to the liver and stomach, and has the function of protecting the liver and stomach.

The embodiments of the present invention are described in detail above, but the contents described are only preferred embodiments of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of the patent coverage of the present invention.

Claims (10)

1. A composition having anti-hangover effect, characterized in that: the material comprises the following raw materials in parts by weight: 100-125 parts of glutathione and 10-20 parts of nervonic acid.

2. The composition having anti-hangover effect as claimed in claim 1, wherein: the adhesive also comprises the following raw materials in parts by weight: 10-20 parts of glycerophosphorylcholine.

3. The composition having anti-hangover effect according to claim 2, wherein: the adhesive also comprises the following raw materials in parts by weight: 50-60 parts of curcumin, 10-15 parts of dihydroquercetin and 100-150 parts of gamma-cyclodextrin.

4. A composition having anti-hangover effect as claimed in claim 3, wherein: the adhesive also comprises the following raw materials in parts by weight: 50-60 parts of fructose.

5. The composition having anti-hangover effect as claimed in claim 4, wherein: the composition with the effects of dispelling the effects of alcohol and resisting hangover comprises the following raw materials in parts by weight: 125 parts of glutathione, 60 parts of curcumin, 15 parts of dihydroquercetin, 20 parts of nervonic acid, 20 parts of glycerophosphorylcholine, 145 parts of gamma-cyclodextrin and 56 parts of fructose.

6. A method for preparing the composition having anti-hangover effect according to any one of claims 1 to 2, characterized in that: the method comprises the following steps:

(1) Mixing 10-20 parts by weight of nervonic acid, 0-20 parts by weight of glycerophosphorylcholine and 100-125 parts by weight of glutathione;

(2) Adding 100-150 parts by weight of filler, mixing, preparing into soft material with water as wetting agent, sieving with 20 mesh sieve to obtain wet granule, drying, grading, and tabletting to obtain composition with effects of relieving hangover;

Wherein the filler adopts one or more of starch, lactose, dextrin, sucrose, pregelatinized starch, microcrystalline cellulose and inorganic salts.

7. A method for preparing the composition having anti-hangover effect according to any one of claims 3 to 5, characterized in that: the method comprises the following steps:

(1) Mixing 10-20 parts by weight of nervonic acid, 10-20 parts by weight of glycerophosphorylcholine, 10-20 parts by weight of gamma-cyclodextrin and 100-125 parts by weight of glutathione, adding 300 parts by weight of water, dissolving and uniformly mixing, and stirring for 2 hours to obtain a wall material coating liquid;

(2) Mixing 10-15 parts by weight of dihydroquercetin with 50-60 parts by weight of curcumin, adding the mixture into the wall material coating liquid obtained in the step (1), uniformly mixing, and performing freeze drying after treatment by a microcapsule granulator to obtain a microencapsulated composition;

(3) Adding 80-140 parts by weight of gamma-cyclodextrin into the microencapsulated composition obtained in the step (2), uniformly mixing, preparing a soft material by using water as a wetting agent, preparing wet granules by using a 20-mesh sieve, drying, granulating, tabletting, and obtaining the composition with the effects of dispelling effects of alcohol and resisting hangover.

8. The method for preparing a composition having anti-hangover effect as claimed in claim 7, wherein: in the step (1), ultrasonic stirring is adopted for 2 hours, wherein the power of the ultrasonic stirring is 60-100W, and the temperature is 55-60 ℃; in the step (2), the vibration frequency of the microcapsule granulator is 1000Hz, the electrode voltage is 500V, the air pressure is 200mbar, the caliber of a nozzle is 400 mu m, and the flow rate is 8mL/min.

9. The method for preparing a composition having anti-hangover effect as claimed in claim 7, wherein: and (3) after finishing the grains, adding 50-60 parts by weight of fructose and 3-5 parts by weight of magnesium stearate, uniformly mixing, and tabletting to obtain the composition with the effects of dispelling the effects of alcohol and resisting hangover.

10. Use of the composition with anti-hangover effect according to any one of claims 1-5 for preparing anti-hangover drugs or health foods.