CN118777213B - Spectral signal detection system, method and related products - Google Patents
Spectral signal detection system, method and related products Download PDFInfo
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- CN118777213B CN118777213B CN202411267698.3A CN202411267698A CN118777213B CN 118777213 B CN118777213 B CN 118777213B CN 202411267698 A CN202411267698 A CN 202411267698A CN 118777213 B CN118777213 B CN 118777213B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/1717—Systems in which incident light is modified in accordance with the properties of the material investigated with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/1717—Systems in which incident light is modified in accordance with the properties of the material investigated with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance
- G01N2021/1725—Modulation of properties by light, e.g. photoreflectance
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The application provides a spectrum signal detection system, a spectrum signal detection method and related products. The spectrum signal detection system comprises a light source, an optical fiber passage and a spectrum detector, wherein the optical fiber passage comprises a light inlet section, a connecting part, a biological detection sensor and a light outlet section, the biological detection sensor comprises a light inlet part, a first sensing part, a second sensing part, a light outlet part and a light transmission part, one end of the light inlet section is communicated with one end of the light inlet part through the connecting part, the light source is positioned at the other end of the light inlet section, one end of the light outlet section is communicated with one end of the light outlet part through the connecting part, the spectrum detector is connected with the other end of the light outlet section, the first sensing part is connected with the other end of the light inlet part and is connected with one end of the light transmission part, and the second sensing part is connected with the other end of the light outlet part and is connected with the other end of the light transmission part. The biological detection sensor is U-shaped, and two sensing parts are symmetrically arranged, so that background liquid interference is eliminated, and detection accuracy is improved.
Description
Technical Field
The application relates to the technical field of biological detection, in particular to a spectrum signal detection system, a spectrum signal detection method and related products.
Background
The optical surface plasmon resonance (Surface Plasmon Resonance, SPR) detection technology is a novel biochemical analysis means extremely sensitive to small refractive index changes, and any biochemical reaction inevitably causes the small refractive index changes of the detection environment, so that the SPR technology can be widely applied to various biochemical detection fields.
At present, when a single SPR sensor detects, the detection result is often inaccurate due to interference of the temperature of background liquid, molecules in the background liquid, the concentration of the background liquid and the like.
Disclosure of Invention
In view of this, the present application provides a spectrum signal detection system and related products, which can improve the accuracy of detection.
In a first aspect, the present application provides a spectral signal detection system comprising:
A light source;
The optical fiber path comprises an incident light section, a connecting part, a biological detection sensor and an emergent light section, wherein the biological detection sensor comprises an incident light part, a first sensing part, a second sensing part, an emergent light part and a light transmission part, one end of the incident light section is communicated with one end of the incident light part through the connecting part, the light source is positioned at the other end of the incident light section, one end of the emergent light section is communicated with one end of the emergent light part through the connecting part, and the spectrum detector is connected with the other end of the emergent light section;
the spectrum detector is used for collecting a target spectrum signal of emergent light from the biological detection sensor, determining a first formant wavelength and a second formant wavelength of the target spectrum signal, determining a target variation characteristic value according to the first formant wavelength and the second formant wavelength, and determining the target concentration of the target molecule according to a mapping relation set of the target variation characteristic value and the variation characteristic value, wherein the target spectrum signal is a detection signal of the second metal film surface adsorption detection solution of the second sensing part, the detection signal is used for representing the variation of the absorption capacity of the biological detection sensor on the incident light of the light source when the target molecule is adsorbed on the second metal film surface under the steady state constraint.
In one possible example, the light transmission portion is provided in a bent manner, the first sensing portion and the second sensing portion are symmetrically provided, and the light incident portion and the light emitting portion are symmetrically provided.
In one possible example, the incident light sequentially passes through the light entrance part, the first sensing part, the light transmission part, the second sensing part and the light exit part of the biological detection sensor, and then exits, and the incident light has the same first transmission direction in the light entrance part and the light transmission part, and the incident light has the same second transmission direction in the light exit part and the light transmission part, and the first transmission direction is opposite to the second transmission direction.
In one possible example, the light entrance portion includes a first optical fiber, the first optical fiber including a first cladding and a first core;
The first sensing part comprises a second optical fiber and a first metal film wrapped on the surface of the second optical fiber, the second optical fiber is communicated with the first fiber core, and the incident light enters the second optical fiber from the first fiber core and generates plasma resonance reaction on the first metal film;
The light transmission part comprises a third optical fiber, the third optical fiber comprises a second cladding and a second fiber core, the second fiber core is communicated with one end of the second optical fiber, which is far away from the first fiber core, and the incident light enters the second fiber core through the second optical fiber;
The second sensing part comprises a fourth optical fiber and the second metal film wrapped on the surface of the fourth optical fiber, the fourth optical fiber is communicated with one end of the second fiber core, which is far away from the second optical fiber, and the incident light enters the fourth optical fiber from the second fiber core and generates plasma resonance reaction on the second metal film;
The light emitting part comprises a fifth optical fiber, the fifth optical fiber comprises a third cladding layer and a third fiber core, the third fiber core is communicated with one end of the fourth optical fiber, which is far away from the second fiber core, and incident light enters the third fiber core through the fourth optical fiber and exits into the spectrum detector through the third fiber core.
In one possible example, the first metal film is modified with a blocking agent layer, the blocking agent layer includes a blocking agent, the second metal film is modified with a capturing functional layer, the capturing functional layer includes a target capturing the target molecule and the blocking agent, and the blocking agent is used for blocking a region of the second metal film where the target is not modified.
In one possible example, the first optical fiber, the third optical fiber, and the fifth optical fiber are multimode optical fibers or single mode optical fibers;
the second optical fiber and the fourth optical fiber are multimode optical fibers or single-mode optical fibers or coreless optical fibers or optical fiber structures for exciting evanescent fields on the surfaces of the optical fibers.
In one possible example, the light incident portion includes a first junction region disposed at a side of the first cladding layer adjacent to the first sensing portion, and both ends of the first junction region are respectively connected to the second optical fiber and the first cladding layer;
The light transmission part comprises a second connection region and a third connection region, the second connection region is arranged on one side, close to the first sensing part, of the second cladding layer, two ends of the second connection region are respectively connected with the second optical fiber and the second cladding layer, the third connection region is arranged on one side, close to the second sensing part, of the second cladding layer, and two ends of the third connection region are respectively connected with the fourth optical fiber and the second cladding layer;
The light emitting part comprises a fourth connection area, the fourth connection area is arranged on one side, close to the second sensing part, of the third cladding layer, and two ends of the fourth connection area are respectively connected with the fourth optical fiber and the third cladding layer.
In one possible example, the target variation characterizing value is a relative difference between the first formant wavelength and the second formant wavelength or a division result value;
The change quantity representation value mapping relation set comprises a corresponding relation between a change quantity representation value and the concentration of the target molecule, and the change quantity representation value and the concentration of the target molecule form a positive association relation.
In a second aspect, the present application further provides a spectrum signal detection method, applied to a spectrum detector in a spectrum signal detection system, where the spectrum signal detection system includes a light source, an optical fiber path, and the spectrum detector, the optical fiber path includes a light-in section, a connection portion, a biological detection sensor, and a light-out section, the biological detection sensor includes a light-in portion, a light-out portion, a first sensing portion, a second sensing portion, and a light-in portion, the connection portion is connected to the light-in section and the light-in portion, the connection portion is connected to the light-out section and the light-out portion, the first sensing portion is connected to the light-in portion and the light-out portion, and the second sensing portion is connected to the light-in portion and the light-out portion, respectively, the method includes:
Acquiring a target spectrum signal of emergent light from the biological detection sensor, wherein the target spectrum signal is a detection signal of target molecules in the adsorption detection solution on the surface of the second metal film of the second sensing part under the constraint of a steady state and other conditions;
Determining a first formant wavelength and a second formant wavelength in the target spectrum signal;
Determining a target variation characterization value according to the first formant wavelength and the second formant wavelength, wherein the target variation characterization value is used for characterizing the variation of the absorption capacity of the biological detection sensor on the incident light of the light source under the steady-state constraint of the target molecule adsorbed on the surface of the second metal film;
and determining the target concentration of the target molecule according to the target variation characteristic value and the variation characteristic value mapping relation set.
In a third aspect, the present application further provides a molecular interaction device, where the interaction device is connected to a spectrum detector in a spectrum signal detection system, and the interaction device is configured to obtain a target variation characteristic value from the spectrum detector, and analyze the interaction affinity between a target molecule and a target object of the target molecule according to the target variation characteristic value.
In a fourth aspect, the present application further provides a portable optical fiber biological detection device, where the portable optical fiber biological detection device includes a housing, a display screen, and the spectrum signal detection system, where the spectrum signal detection system is accommodated in the housing, and the display screen is disposed outside the housing and is used for displaying detection information.
The spectrum signal detection system provided by the embodiment changes the direction of light transmission by bending the light transmission part of the biological detection sensor without arranging additional optical components such as a reflector, thereby reducing the manufacturing cost of the biological detection sensor, and symmetrically arranging the first sensing part and the second sensing part on the biological detection sensor, wherein the first sensing part does not modify the target of the target molecule, and seals the target by using a sealing agent, and the second sensing part modifies the target, and due to the bending arrangement of the light transmission part, the incident light of the light source can sequentially pass through the modulation of the first sensing part and the modulation of the second sensing part, the spectrum detector acquires the target spectrum signal of the modulated emergent light, determines the first formant wavelength and the second formant wavelength of the target spectrum signal, analyzes the concentration of the target molecule based on the first formant wavelength and the second formant wavelength, eliminates the interference of background liquid, and is beneficial to improving the detection accuracy. And when the spectrum signal detection system detects the target solution, the biological detection sensor can be directly soaked in the detection solution, and complex sample transfer steps are not needed, so that the detection efficiency is improved.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only some embodiments of the invention, and that other drawings can be obtained from them without inventive effort for a person skilled in the art.
Fig. 1 is a schematic structural diagram of a spectrum signal detection system according to an embodiment of the present application;
FIG. 2 is a schematic diagram of a biological detection sensor according to an embodiment of the present application;
FIG. 3 is a schematic diagram of another spectrum signal detecting system according to an embodiment of the present application;
FIG. 4 is a schematic diagram of a light transmission path according to an embodiment of the present application;
FIG. 5 is a schematic diagram of another bio-detection sensor provided by an embodiment of the present application;
FIG. 6 is a schematic diagram of a spectral signal according to an embodiment of the present application;
FIG. 7 is a schematic diagram of a spectrum signal detection system according to another embodiment of the present application;
FIG. 8 is a schematic diagram of a spectral signal detection system including an interaction device according to an embodiment of the present application;
FIG. 9 is a flowchart of a method for detecting a spectrum signal according to an embodiment of the present application;
Fig. 10 is a schematic structural view of a portable optical fiber biological detection device according to an embodiment of the present application.
Reference numerals illustrate:
The optical spectrum signal detection system 1, the portable optical fiber biological detection device 2, the light source 10, the optical fiber path 20, the optical spectrum detector 30, the light entering section 201, the connection section 202, the biological detection sensor 203, the light exiting section 204, the light entering section 2031, the first sensing section 2032, the second sensing section 2034, the light exiting section 2035, the light transmitting section 2033, the first port 2021, the second port 2022, the third port 2023, the fourth port 2024, the first optical fiber 41, the first cladding 401, the first fiber core 402, the second optical fiber 51, the first metal film 52, the third optical fiber 61, the second cladding 601, the second fiber core 602, the fourth optical fiber 71, the second metal film 72, the fifth optical fiber 81, the third cladding 801, the third fiber core 802, the first joining region 91, the second joining region 92, the third joining region 93, the fourth joining region 94, the detection channel 100, the optical path switcher 110, the interaction instrument 120, the housing 21, and the display screen 22.
Detailed Description
In order that those skilled in the art will better understand the present application, a technical solution in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in which it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The terms first, second and the like in the description and in the claims and in the above-described figures are used for distinguishing between different objects and not necessarily for describing a sequential or chronological order. Furthermore, the terms "comprise" and "have," as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed steps or elements but may include other steps or elements not listed or inherent to such process, method, article, or apparatus.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
In the embodiment of the application, "and/or" describes the association relation of the association objects, which means that three relations can exist. For example, A and/or B may represent three cases, A alone, A and B together, and B alone. Wherein A, B may be singular or plural.
In the embodiment of the present application, the symbol "/" may indicate that the associated object is an or relationship. In addition, the symbol "/" may also denote a divisor, i.e. performing a division operation. For example, A/B may represent A divided by B.
"At least one" or the like in the embodiments of the present application means any combination of these items, including any combination of single item(s) or plural items(s), meaning one or more, and plural means two or more. For example, at least one (one) of a, b or c may represent seven cases a, b, c, a and b, a and c, b and c, a, b and c. Wherein each of a, b, c may be an element or a set comprising one or more elements.
The 'equal' in the embodiment of the application can be used with the greater than the adopted technical scheme, can also be used with the lesser than the adopted technical scheme. When the combination is equal to or greater than the combination, the combination is not less than the combination, and when the combination is equal to or less than the combination, the combination is not greater than the combination.
In order to better understand the solution of the embodiment of the present application, the following describes electronic devices, related concepts and backgrounds that may be related to the embodiment of the present application.
The electronic device according to the embodiment of the present application may include various handheld devices, vehicle-mounted devices, wearable devices, computing devices or other processing devices connected to a wireless modem, and various forms of User Equipment (UE), mobile Station (MS), electronic device (TERMINAL DEVICE), etc. For convenience of description, the above-mentioned devices are collectively referred to as electronic devices. The electronic device may also be a spectral detector in a fiber optic biological detection system.
Referring to fig. 1, fig. 2 and fig. 3, fig. 1 is a schematic structural diagram of a spectrum signal detection system according to an embodiment of the present application, fig. 2 is a schematic structural diagram of a biological detection sensor according to an embodiment of the present application, and fig. 3 is a schematic structural diagram of another spectrum signal detection system according to an embodiment of the present application. The spectral signal detection system 1 of the present embodiment includes a light source 10, an optical fiber path 20, and a spectral detector 30, the optical fiber path 20 includes an light-entering section 201, a connecting section 202, a biological detection sensor 203, and a light-exiting section 204, and the biological detection sensor 203 includes a light-entering section 2031, a first sensing section 2032, a second sensing section 2034, a light-exiting section 2035, and a light transmission section 2033.
The light source 10 is configured to emit light, and the emitted light is broadband white light, one end of the light incident section 201 is communicated with one end of the light incident section 2031 through the connection portion 202, the light source 10 is located at the other end of the light incident section 201, one end of the light emergent section 204 is communicated with one end of the light emergent section 2035 through the connection portion 202, the spectrum detector 30 is connected with the other end of the light emergent section 204, the first sensing portion 2032 is connected with the other end of the light incident section 2031, and is connected with one end of the light transmitting portion 2033, and the second sensing portion 2034 is connected with the other end of the light emergent section 2035 and is connected with the other end of the light transmitting portion 2033.
Among them, broadband white light is understood as a composite light formed by mixing a plurality of monochromatic lights, and the spectrum range thereof covers most or all of the wavelength bands of visible light.
The connection portion 202 may be an optical fiber connector, the connection portion 202 includes a first port 2021, a second port 2022, a third port 2023 and a fourth port 2024, the light-in section 201 is connected to the first port 2021, the light-in section 2031 is connected to the second port 2022, the light-out section 2035 is connected to the third port 2023, the light-out section 204 is connected to the fourth port 2024, the first port 2021 is communicated with the second port 2022, and the third port 2023 is communicated with the fourth port 2024.
The spectrum detector 30 is configured to collect a target spectrum signal of the outgoing light from the biological detection sensor 203, determine a first formant wavelength and a second formant wavelength of the target spectrum signal, determine a target variation characterizing value according to the first formant wavelength and the second formant wavelength, and determine a target concentration of a target molecule according to a mapping relation set of the target variation characterizing value and the variation characterizing value, where the target spectrum signal is a detection signal of the second sensing portion 2034, in which the surface of the second metal film 72 adsorbs the target molecule in the detection solution under a steady state and other condition constraints, and the target variation characterizing value is used to characterize a variation of the absorption capacity of the biological detection sensor 203 on the surface of the second metal film 72 to the incident light of the light source 10 when the target molecule is adsorbed under the steady state constraint. Other conditions include temperature conditions, background fluid composition conditions, background fluid concentration conditions, PH conditions, background fluid composition conditions including the composition of the background fluid and interactions between the compositions. Other conditions may affect the refractive index of the metal film surface and thus the accuracy of the detection.
The target molecule may be an antigen, and the target may be an antibody, which is not limited herein, and the target may react with the target molecule, so as to stably adsorb the target molecule to the second sensing portion 2034.
It will be appreciated that in other embodiments of the application, the antibodies may be immobilized to the surface of the metal film by a bio-crosslinking agent, e.g., dopamine, carbodiimide, etc.
Alternatively, the types of antibodies are not limited, and modification of different types of antibodies may enable the biological detection sensor 203 to be a different specific detection tool, for example, specific detection of proteins, nucleic acids, cells, and the like.
Preferably, the shape of the biological detection sensor 203 is "U" shaped or approximately "U" shaped. Specifically, the light transmitting portion 2033 is bent, and the bent shape is "U" or approximately "U", and the first sensing portion 2032, the second sensing portion 2034, the light incident portion 2031 and the light emergent portion 2035 are all arranged straight.
Alternatively, the first sensing part 2032 and the second sensing part 2034 are symmetrically disposed and parallel or approximately parallel to each other, and the light incident part 2031 and the light emitting part 2035 are symmetrically disposed and parallel or approximately parallel to each other.
In this embodiment, the propagation paths of the incident light and the outgoing light inside the biological detection sensor 203 do not overlap each other, so that the incident light and the outgoing light do not need to be split by using a spectral coupler, so that the detection result of the biological detection sensor 203 is more accurate and reliable.
Referring to fig. 4, fig. 4 is a schematic diagram of a light transmission path provided by an embodiment of the present application, the transmission path of incident light emitted by the light source 10 is as follows, the incident light enters the light entering section 201 in the optical fiber path 20, enters the light entering section 2031 after passing through the connecting section 202, enters the first sensing section 2032 after passing through the light entering section 2031, enters the light transmitting section 2033 after being modulated by the first sensing section 2032, enters the second sensing section 2034 after passing through the light transmitting section 2033, enters the light exiting section 2035 after being modulated by the second sensing section 2034, enters the light exiting section 204 after passing through the light exiting section 2035, and exits through the light exiting section 204. The incident light has the same first transmission direction in the light entrance portion 2031 and the light transmission portion 2033, and the incident light has the same second transmission direction in the light exit portion 2035 and the light transmission portion 2033, and the first transmission direction is opposite to the second transmission direction.
Referring to fig. 5, fig. 5 is a schematic diagram of another biological detection sensor provided by the embodiment of the application, the light input portion 2031 includes a first optical fiber 41, the first optical fiber 41 includes a first cladding 401 and a first fiber core 402, the first sensing portion 2032 includes a second optical fiber 51 and a first metal film 52 wrapped on the surface of the second optical fiber 51, the second optical fiber 51 is communicated with the first fiber core 402, the incident light enters the second optical fiber 51 from the first fiber core 402 and generates a plasmon resonance reaction on the first metal film 52, the light transmitting portion 2033 includes a third optical fiber 61, the third optical fiber 61 includes a second cladding 601 and a second fiber core 602, the second optical fiber 602 is communicated with one end of the second optical fiber 51 facing away from the first fiber core 402, the incident light enters the second fiber core 602 through the second optical fiber 51, the second sensing portion 2034 includes a fourth optical fiber 71 and a fourth metal film 71 wrapped on the surface of the first fiber core 402, the incident light enters the second optical fiber 51 from the second fiber core 71 and exits the third fiber core 602 through the second metal film 71, the third optical fiber core 71 is communicated with the third optical fiber core 71, the third optical fiber 71 exits the third fiber core 602 through the third cladding 71, and the third optical fiber 71 exits the fourth optical fiber 71 from the third fiber core 602, and the fourth optical fiber 71 exits the fourth optical fiber 71 through the third fiber core 71 and the third fiber 602, and the third optical fiber 71 exits the third optical fiber 71 and the third fiber enters the third optical fiber 602 through the third fiber core 71 and the third fiber 71.
The light-in section 201 includes a sixth optical fiber connected to the first port 2021 of the connection portion 202, and the light-out section 204 includes a seventh optical fiber connected to the fourth port 2024 of the connection portion 202.
The first optical fiber 41, the third optical fiber 61, the fifth optical fiber 81, the sixth optical fiber and the seventh optical fiber are multimode optical fibers or single-mode optical fibers, and the second optical fiber 51 and the fourth optical fiber 71 are multimode optical fibers or single-mode optical fibers or coreless optical fibers or other optical fiber structures for exciting an evanescent field on the surface of the optical fiber.
Alternatively, the light incident portion 2031 includes a first connection region 91, the first connection region 91 is disposed on a side of the first cladding 401 adjacent to the first sensing portion 2032, two ends of the first connection region 91 are respectively connected to the second optical fiber 51 and the first cladding 401, the light transmission portion 2033 includes a second connection region 92 and a third connection region 93, the second connection region 92 is disposed on a side of the second cladding 601 adjacent to the first sensing portion 2032, two ends of the second connection region 92 are respectively connected to the second optical fiber 51 and the second cladding 601, the third connection region 93 is disposed on a side of the second cladding 601 adjacent to the second sensing portion 2034, two ends of the third connection region 93 are respectively connected to the fourth optical fiber 71 and the second cladding 601, the light emergent portion 2035 includes a fourth connection region 94, the fourth connection region 94 is disposed on a side of the third cladding 801 adjacent to the second sensing portion 2034, and two ends of the fourth connection region 94 are respectively connected to the fourth optical fiber 71 and the third cladding 801.
Alternatively, the first metal film 52 and the second metal film 72 are composed of one or more of elemental metals, or mixed alloys, or multi-layered metal composite layers or semiconductor layers, and have abundant free electrons. The preparation method of the metal film comprises, but is not limited to, adopting a thermal evaporation vacuum coating method to deposit on the surface of the optical fiber.
In one possible example, the surface of the first metal film 52 is modified with a blocking agent layer, the blocking agent layer includes a blocking agent, the blocking agent layer blocks the first metal film 52 from adsorbing the target molecules, and thus the refractive index of the surface of the first metal film 52 is not affected by the adsorption state of the target molecules, and is only affected by other conditions, the surface of the second metal film 72 is modified with a capturing functional layer, the capturing functional layer includes a target object capturing the target molecules and a blocking agent, the blocking agent is used for blocking the area of the second metal film 72 where the target object is not modified, and the refractive index of the surface of the second metal film 72 is affected by the adsorption state of the target molecules and other conditions. Preferably, the target of the target molecule is first modified on the second metal film 72, and then blocked with a blocking agent to form a capture functional layer. The sequence of modification of the blocking agent and the target on the surface of the second metal film 72 is to modify the target first and then to block the target with the blocking agent.
Wherein, the sealant layer can prevent the target molecules from being fixed to the area of the second metal film 72 where the target is not modified, thereby avoiding deviation in detection caused by uneven distribution of the target molecules adsorbed on the surface of the second metal film 72, and the sealant layer can prevent other molecules in the analyte from being adsorbed on the surface of the second metal film 72, thereby affecting the accuracy of detection.
Wherein the components of the blocking agent include, but are not limited to, bovine serum albumin.
Referring to fig. 6, fig. 6 is a schematic diagram of a spectrum signal provided by the embodiment of the present application, in which a target spectrum curve corresponding to the target spectrum signal is shown in the figure, the ordinate represents an outgoing light intensity, the unit cd candelas, the abscissa represents a light wavelength, and the unit nm, the target spectrum curve includes two trough regions, the trough regions are bending transition regions of the target spectrum curve, and the trough regions are open along a direction of increasing numerical value of the ordinate, wherein when an incident light passes through the first sensing portion 2032, plasmon resonance is generated on the surface of the first metal film 52 of the first sensing portion 2032, energy of photons of a first wavelength section of the incident light is absorbed to form a first trough region of a spectrum of the outgoing light, and as a result of the absorption of target molecules by the surface of the second metal film 72 of the second sensing portion 2034, the incident light absorbs energy of photons of a second wavelength section of the incident light when the incident light passes through the second sensing portion 2034, and the energy of photons of the second wavelength section of the incident light is greater than the value of any wavelength section of the second spectrum.
Wherein, as the number of target molecules adsorbed on the surface of the second metal film 72 increases, the refractive index of the surface of the second metal film 72 increases, the second valley regions move right and the wavelengths of the peaks of the second valley regions also increase.
The wavelength value corresponding to the peak of the first trough region is the first resonance peak wavelength, and the wavelength value corresponding to the peak of the second trough region is the second resonance peak wavelength. The target variable quantity representation value is a relative difference value between the first formant wavelength and the second formant wavelength or a division operation result value, and the variable quantity representation value mapping relation set comprises a corresponding relation between the variable quantity representation value and the concentration of the target molecule, wherein the variable quantity representation value and the concentration of the target molecule form a positive association relation. The target variable quantity representation value is determined according to the first formant wavelength and the second formant wavelength, so that the interference of background liquid is eliminated, and the detection accuracy is improved.
In one possible example, the spectral signal detection system 1 is a single channel system, i.e. the spectral signal detection system 1 is provided with only a single bio-detection sensor 203.
In one possible example, the spectroscopic signal detection system 1 is a multichannel system, i.e. the spectroscopic signal detection system 1 is provided with a plurality of simultaneously detecting biological detection sensors 203.
Referring to fig. 7, fig. 7 is a schematic structural diagram of a spectrum signal detection system according to another embodiment of the application. The spectrum signal detection system 1 includes a light source 10, a detection channel 100, an optical path switch 110, and a spectrum detector 30.
Alternatively, the number of detection channels 100 may be, but is not limited to, two, or three, or four, or five, or other greater numbers, etc.
Wherein, single detection channel 100 includes light entering section 201, connecting portion 202, biological detection sensor 203 and light emitting section 204, and light emitting section 204 in every detection channel 100 connects light path switch 110, and light path switch 110 connects spectral detector 30, and light entering section 201 communicates biological detection sensor 203 through connecting portion 202, and light emitting section 204 communicates biological detection sensor 203 through connecting portion 202.
The optical path switch 110 is used for switching the detection channels 100 in a time-sharing manner, so that only the detection signal of one detection channel 100 is transmitted to the spectrum detector 30 at the same time.
It will be appreciated that in other embodiments of the present application, the plurality of connectors 202 may also be provided integrally, i.e., the plurality of connectors 202 may be provided in an integrated fiber optic splicing instrument.
Optionally, a single or multiple light sources 10 are provided in the spectral signal detection system 1, where the single light source 10 is capable of transmitting light to one or multiple light-entering segments 201, which is not limited by the present application.
As can be appreciated, compared to the comparative embodiment in which one spectrum sensor 30 is separately configured for each biological detection sensor 203, in the present embodiment, the spectrum signal detection system 1 collects detection signals of the emitted light of different biological detection sensors 203 in a time-sharing manner through the optical path switch 110, and transmits the detection signals of different biological detection sensors 203 to the spectrum sensors 30 in a time-sharing manner, and by controlling the switching speed of the optical path switch 110, it can be ensured that the detection signals of the respective spectrum sensors 30 can be processed timely and effectively. The spectrum signal detection system 1 can save cost and reduce complexity and maintenance difficulty of the system while guaranteeing detection efficiency. And, because there is no need to provide a plurality of spectrum detectors 30, the spectrum signal detection system 1 provided in this embodiment is convenient for performing a miniaturized design, and reduces the occupied space of the spectrum signal detection system 1, so that the application scenario of the spectrum signal detection system 1 is more flexible and wider.
Referring to fig. 8, fig. 8 is a schematic structural diagram of a spectrum signal detection system including an interaction device according to an embodiment of the present application, wherein the interaction device 120 is connected to a spectrum detector 30 in the spectrum signal detection system 1, the spectrum detector 30 is used for sending a target variation characteristic value to the interaction device 120, and the interaction device 120 is used for obtaining the target variation characteristic value from the spectrum detector 30 and analyzing the interaction affinity between a target molecule and a target object of the target molecule according to the target variation characteristic value.
The interaction instrument 120 performs biosensing analysis based on the SPR principle, and does not need to perform labeling or purifying various biological components, thereby reducing errors possibly caused by labeling or purifying. The interaction meter 120 can measure the interaction process between various biomolecules such as polypeptides, proteins, oligonucleotides, oligosaccharides, and viruses, bacteria, cells, small molecule compounds in real time, in situ and dynamically by the spectroscopic signal detection system 1 under natural conditions, thereby obtaining more real and accurate detection results.
Referring to fig. 9, fig. 9 is a flowchart of a spectrum signal detection method provided by an embodiment of the present application, which is applied to a spectrum detector in a spectrum signal detection system, wherein the spectrum signal detection system includes a light source, an optical fiber path and the spectrum detector, the optical fiber path includes a light-in section, a connection portion, a biological detection sensor and a light-out section, the biological detection sensor includes a light-in portion, a light-out portion, a first sensing portion, a second sensing portion and a light-in portion, the connection portion is respectively connected to the light-in section and the light-in portion, the connection portion is respectively connected to the light-out section and the light-out portion, the first sensing portion is respectively connected to the light-in portion and the light-out portion, and the second sensing portion is respectively connected to the light-in portion and the light-out portion, the method includes:
S901, acquiring a target spectrum signal of emergent light from the biological detection sensor, wherein the target spectrum signal is a detection signal of target molecules in the adsorption detection solution on the surface of the second metal film of the second sensing part under the constraint of a steady state and other conditions.
S902, determining a first formant wavelength and a second formant wavelength in the target spectrum signal;
S903, determining a target variable quantity representation value according to the first formant wavelength and the second formant wavelength, wherein the target variable quantity representation value is used for representing the variable quantity of the absorption capacity of the biological detection sensor on the incident light of the light source under the steady-state constraint of the target molecule adsorbed on the surface of the second metal film;
S904, determining the target concentration of the target molecule according to the target variation characteristic value and the variation characteristic value mapping relation set.
It can be seen that, in this example, the signal processing device collects the target spectrum signal of the outgoing light of the biological detection sensor, determines the first formant wavelength and the second formant wavelength in the target spectrum signal, analyzes the concentration of the target molecule based on the first formant wavelength and the second formant wavelength, and eliminates the interference of the background liquid, thereby being beneficial to improving the detection accuracy.
In one possible example, an interactor is coupled to the spectral detector, the interactor is configured to obtain a target variation characteristic from the spectral detector, and analyze the magnitude of interaction affinity between the target molecule and a target of the target molecule based on the target variation characteristic.
In one possible example, the light transmission portion is provided in a bent manner, the first sensing portion and the second sensing portion are symmetrically provided, and the light incident portion and the light emitting portion are symmetrically provided.
In one possible example, the incident light sequentially passes through the light entrance part, the first sensing part, the light transmission part, the second sensing part and the light exit part of the biological detection sensor, and then exits, and the incident light has the same first transmission direction in the light entrance part and the light transmission part, and the incident light has the same second transmission direction in the light exit part and the light transmission part, and the first transmission direction is opposite to the second transmission direction.
In one possible example, the light incident portion includes a first optical fiber including a first cladding and a first core, the first sensing portion includes a second optical fiber and a first metal film wrapped on a surface of the second optical fiber, the second optical fiber is communicated with the first core, the incident light enters the second optical fiber from the first core and generates a plasmon resonance reaction on the first metal film, the light transmitting portion includes a third optical fiber including a second cladding and a second core, the second core is communicated with an end of the second optical fiber facing away from the first core, the incident light enters the second core through the second optical fiber, the second sensing portion includes a fourth optical fiber and a second metal film wrapped on a surface of the fourth optical fiber, the fourth optical fiber is communicated with an end of the second optical fiber facing away from the second core, the light enters the third core, the third optical fiber exits the third core through the third metal film, and the third optical fiber enters the fifth optical fiber via the third core, and the third metal film is communicated with the fourth optical fiber.
In one possible example, the first metal film is modified with a blocking agent layer, the blocking agent layer includes a blocking agent, the second metal film is modified with a capturing functional layer, the capturing functional layer includes a target capturing the target molecule and the blocking agent, and the blocking agent is used for blocking a region of the second metal film where the target is not modified.
In one possible example, the first, third and fifth optical fibers are multimode or single mode optical fibers, and the second and fourth optical fibers are multimode or single mode or coreless or other optical fiber structures that excite an evanescent field at the surface of the optical fibers.
In one possible example, the light incident portion includes a first linking region disposed on a side of the first cladding layer adjacent to the first sensing portion, and two ends of the first linking region are respectively connected to the second optical fiber and the first cladding layer, the light transmitting portion includes a second linking region disposed on a side of the second cladding layer adjacent to the first sensing portion, and two ends of the second linking region are respectively connected to the second optical fiber and the second cladding layer, and two ends of the third linking region are disposed on a side of the second cladding layer adjacent to the second sensing portion, and two ends of the third linking region are respectively connected to the fourth optical fiber and the second cladding layer, and the light emitting portion includes a fourth linking region disposed on a side of the third cladding layer adjacent to the second sensing portion, and two ends of the fourth linking region are respectively connected to the fourth optical fiber and the third cladding layer.
In one possible example, the target variation characterizing value is a relative difference value or a division result value between the first formant wavelength and the second formant wavelength, and the variation characterizing value mapping relation set includes a corresponding relation between a variation characterizing value and the concentration of the target molecule, and the variation characterizing value and the concentration of the target molecule form a positive association relation.
Referring to fig. 10, fig. 10 is a schematic structural diagram of a portable optical fiber biological detection device according to an embodiment of the application. The application also provides a portable optical fiber biological detection device 2, the portable optical fiber biological detection device 2 comprises a shell 21, a display screen 22 and a spectrum signal detection system 1, the spectrum signal detection system 1 is accommodated in the shell 21, and the display screen 22 is arranged outside the shell 21 and is used for displaying detection information.
Alternatively, the portable fiber optic biological detection device 2 is a hand-held device.
The display 22 may be, but is not limited to, concentration of the target molecule in the detection solution, or affinity information between the target molecule and a target of the target molecule, or other characteristic information.
It will be appreciated that conventional sensors are disadvantageous for the popularization of hand-held devices due to the large volume, complex testing procedures, etc. The portable optical fiber biological detection device 2 provided in this embodiment has a simple structure and a small volume, so that the biological detection sensor 203 can be applied to the portable optical fiber biological detection device 2, and a user can detect a biological sample anytime and anywhere without carrying a heavy detection setting, thereby improving the convenience and flexibility of the detection process.
The spectrum detector provided by the embodiment is used for executing the spectrum signal detection method, so that the same effect as that of the implementation method can be achieved.
In case an integrated unit is employed, the spectral detector may comprise a processing module, a storage module and a communication module. The processing module may be configured to control and manage an action of the spectrum detector, for example, may be configured to support the spectrum detector to perform the steps performed by the functional unit. The memory module may be used to support the spectrum detector to perform storing program code and data, etc. And the communication module can be used for supporting the communication between the spectrum detector and other devices.
Wherein the processing module may be a processor or a controller. Which may implement or perform the various exemplary logic blocks, modules and circuits described in connection with this disclosure. A processor may also be a combination that performs computing functions, e.g., including one or more microprocessors, digital Signal Processing (DSP) and a combination of microprocessors, and the like. The memory module may be a memory. The communication module can be a radio frequency circuit, a Bluetooth chip, a Wi-Fi chip and other equipment which interact with other electronic equipment.
The embodiment of the application also provides a computer storage medium, wherein the computer storage medium stores a computer program for electronic data exchange, and the computer program makes a computer execute part or all of the steps of any one of the method embodiments described in the method embodiment, and the computer includes a spectrum detector.
Embodiments of the present application also provide a computer program product comprising a non-transitory computer-readable storage medium storing a computer program operable to cause a computer to perform part or all of the steps of any one of the methods described in the method embodiments above. The computer program product may be a software installation package, said computer comprising a control platform.
It should be noted that, for simplicity of description, the foregoing method embodiments are all described as a series of acts, but it should be understood by those skilled in the art that the present application is not limited by the order of acts described, as some steps may be performed in other orders or concurrently in accordance with the present application. Further, those skilled in the art will also appreciate that the embodiments described in the specification are all preferred embodiments, and that the acts and modules referred to are not necessarily required for the present application.
In the foregoing embodiments, the descriptions of the embodiments are emphasized, and for parts of one embodiment that are not described in detail, reference may be made to related descriptions of other embodiments.
In the several embodiments provided by the present application, it should be understood that the disclosed apparatus may be implemented in other manners. For example, the above-described apparatus embodiments are merely illustrative, such as the above-described division of units, merely a division of logic functions, and there may be additional manners of dividing in actual implementation, such as multiple units or components may be combined or integrated into another system, or some features may be omitted, or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with each other may be an indirect coupling or communication connection via some interfaces, devices or units, or may be in electrical or other forms.
The units described above as separate components may or may not be physically separate, and components shown as units may or may not be physical units, may be located in one place, or may be distributed over a plurality of network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
In addition, each functional unit in the embodiments of the present application may be integrated in one processing unit, or each unit may exist alone physically, or two or more units may be integrated in one unit. The integrated units may be implemented in hardware or in software functional units.
The integrated units described above, if implemented in the form of software functional units and sold or used as stand-alone products, may be stored in a computer readable memory. Based on such understanding, the technical solution of the present application may be embodied in essence or a part contributing to the prior art or all or part of the technical solution in the form of a software product stored in a memory, comprising several instructions for causing a computer device (which may be a personal computer, a server or a network device, etc.) to perform all or part of the steps of the above-mentioned method of the various embodiments of the present application. The Memory includes a U disk, a Read-Only Memory (ROM), a random access Memory (RAM, random Access Memory), a removable hard disk, a magnetic disk, or an optical disk, etc. which can store the program codes.
Those of ordinary skill in the art will appreciate that all or a portion of the steps of the various methods of the above embodiments may be implemented by a program that instructs associated hardware, the program may be stored in a computer readable memory, the memory may include a flash disk, a read only memory, a random access memory, a magnetic or optical disk, etc.
The foregoing has outlined rather broadly the more detailed description of embodiments of the application, wherein the principles and embodiments of the application are explained in detail using specific examples, the above examples being provided solely to facilitate the understanding of the method and core concepts of the application; meanwhile, as those skilled in the art will have variations in the specific embodiments and application scope in accordance with the ideas of the present application, the present description should not be construed as limiting the present application in view of the above.
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| CN118425105B (en) * | 2024-07-05 | 2024-09-20 | 深圳大学 | Signal processing method based on optical fiber biological detection sensor and related products |
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