CN1185258C - Trivalent chromium compound and its application - Google Patents

Abstract

The invention provides a trivalent chromium compound for treating diabetes, which is a compound formed by a trivalent chromium compound and lactoferrin. The trivalent chromium compound can be added into a milk product to obtain the trivalent chromium milk product. The trivalent chromium compound or the trivalent chromium dairy product can be eaten or taken by diabetics, and can effectively reduce blood sugar, blood fat and cholesterol of the diabetics.

Description

Trivalent chromium compound and its application

This application is a divisional application filed on May 19, 2000 with the title of "trivalent chromium complex, its milk product and its manufacturing method", and the latter's application number is 00106264.9.

technical field

The invention relates to a novel trivalent chromium compound and its application. More specifically, the present invention relates to a trivalent chromium complex for treating diabetes and its application in preparations for treating diabetes.

Background technique

As the human diet becomes richer and richer, the number of diabetic patients has increased significantly. About 90% of diabetics in Taiwan belong to non-insulin-dependent type or type 2 diabetes. According to research results, the chromium content in serum of type 2 diabetes patients is about 0.5 ng/ml, far from It is lower than the chromium content in normal human serum (0.5ng/ml).

A lot of labor, pregnancy, obesity, old age, alcoholism, surgery, or disease and other conditions will increase the excretion of chromium in the body, resulting in a lack of chromium, making it difficult for blood sugar to enter cells, and causing clinical symptoms of type 2 diabetes.

According to different research results, the benefits of organic chromium to the human body include: (1) increasing the function of insulin, (2) promoting normal glucose metabolism, (3) increasing glucose tolerance, (4) increasing protein production, (5) ) reduces the chance of cardiovascular disease by increasing high-density lipoprotein cholesterol, (6) participates in lipid regulation, (7) reduces the chance of maturity-onset diabetes, (8) reduces cardiovascular disease The probability of occurrence, (9) can assist the weight control of obese people.

Suitable organic chromium includes: chromium picolinate, chromium nicotinate, chromium GTF, chromium yeast extract, etc.

Organic chromium supplementation can help treat diabetes caused by chromium deficiency. For ordinary adults, chromium can be taken together with other multivitamins and minerals as a personal nutrient supplement.

U.S. Patent No. 4,923,855 discloses about "SYNTHETIC GTF CHROMIUM MATERIAL AND PROCSSTHEREFOR" (SYNTHETIC GTF CHROMIUM MATERIAL AND PROCFSTHEREFOR), reacting trivalent chromium salt with nicotine acid (nictotinic acid) to obtain glucose tolerance factor) new chromium-containing products.

Contents of the invention

The invention relates to a novel trivalent chromium complex, which can be used to control diabetes. The manufacturing method of the trivalent chromium complex of the present invention mainly includes adding lactoferrin or whey protein with the trivalent chromium compound, and heat-treating to form the trivalent chromium complex. The trivalent chromium complex of the present invention can be added to dairy products to obtain trivalent chromium dairy products.

The dairy products referred to in the present invention are selected from fresh milk, long-lasting milk, concentrated milk, cheese, or milk powder including various mammals (such as humans, cows, sheep, horses, deer, camels, etc.).

The lactoferrin of the present invention is bovine lactoferrin, goat lactoferrin, sheep lactoferrin, human lactoferrin, or lactoferrin of other mammals. The whey protein of the present invention refers to unpurified bovine milk, goat milk, sheep milk, human milk, or whey protein of other mammals.

The trivalent chromium complex of the invention can be added to dairy products to obtain trivalent chromium dairy products, which can be effectively absorbed and utilized by human body. Regular consumption of the trivalent chromium dairy products of the present invention not only has the nutrition of general dairy products, but also can effectively supplement the deficiency of organic chromium in the body, and can effectively control blood sugar, blood fat, and cholesterol levels in type 2 diabetes patients.

The trivalent chromium dairy product of the present invention contains a trivalent chromium complex, which is an organic trivalent chromium complex formed by heat treatment of trivalent chromium and lactoferrin, which can promote carbohydrates, proteins, and Normal metabolism of fat. Lactoferrin is a single-chain glycoprotein (glycoprotein) with the ability to bind metal ions. ^It has a high affinity for iron ions (Fe ³⁺ ), and can bind to V ³⁺ , Cr ³⁺ , Mn ^{3+ +} , and other metal ions, and can still maintain the ability to bond with iron ions in an acidic environment with a pH of 2 to 3. Each molecule of lactoferrin can be combined with two iron ions, and the binding site of chromium is the same as that of iron.

Lactoferrin mainly exists in the whey part of milk, and also exists in epithelial secretions such as neutrophils, tears, saliva, pancreatic juice, vaginal secretions, semen, and accessory testes. Protein has broad-spectrum antibacterial function and can regulate the immune system in the body.

The trivalent chromium dairy product of the present invention is a kind of food or health food, which can be taken or eaten by patients with type 2 diabetes, can effectively supplement the deficiency of organic chromium in the body of patients, and can promote the normalization of carbohydrates, proteins, and fats. Metabolism, to relieve various discomforts of diabetic patients.

The manufacture method of the trivalent chromium complex of the present invention generally comprises the following steps:

(a) providing at least one trivalent chromium compound selected from the group consisting of chromium trichloride hexahydrate, chromium trichloride, chromium acetate, chromium (III) nitrate, chromium oxide (chromium (III) oxide), chromium(III) sulfate, or other inorganic or organic chromium species; (b) added lactoferrin or whey protein; and (c) heat-treated to form a trivalent chromium complex The heat treatment means that the heating temperature is in the range of 37° C. to 95° C., and stirring is applied during the heating process to fully mix to obtain the trivalent chromium complex.

The manufacture method of the trivalent chromium milk product of the present invention generally comprises the following steps:

(a) providing at least one trivalent chromium compound selected from the group consisting of chromium trichloride hexahydrate, chromium trichloride, chromium acetate, chromium (III) nitrate, chromium oxide (chromium (III) oxide), chromium(III) sulfate, or other inorganic or organic chromium species; (b) added lactoferrin or whey protein; (c) heat-treated to form a trivalent chromium complex , the heat treatment means that the heating temperature is in the range of 37°C to 95°C, and stirring is applied during the heating process to fully mix; and (d) adding the trivalent chromium complex to dairy products to obtain three Chromium dairy products.

In the manufacture method of the present invention, the trivalent chromium raw material used can be inorganic chromium or organic chromium, such as chromium trichloride hexahydrate, chromium trichloride, chromium acetate, chromium nitrate (chromium (III) nitrate), oxidation Chromium(III) oxide, chromium(III) sulfate or other inorganic or organic chromium.

In the heat treatment in the manufacturing method of the present invention, the heating temperature may be in the range of about 37°C to 95°C, preferably 60°C to 80°C. During the heating process, it can be properly stirred to make it well mixed.

The source of lactoferrin may be bovine lactoferrin, goat lactoferrin, sheep lactoferrin, human lactoferrin, or lactoferrin from other mammals. Since lactoferrin mainly exists in the whey part of milk, unpurified whey protein products of various mammals can also be used in the present invention.

The present invention also relates to the application of the trivalent chromium compound in the preparation of preparations for treating diabetes. The trivalent chromium compound is added to lactoferrin or whey protein by adding the trivalent chromium compound and heat-treated at 60°C to 80°C. The formed trivalent chromium complex, the trivalent chromium compound is selected from chromium trichloride hexahydrate, chromium trichloride, chromium acetate, chromium nitrate, chromium oxide, or chromium sulfate, and the lactoferrin or whey protein selected from bovine lactoferrin, goat lactoferrin, ovine lactoferrin, human lactoferrin, unpurified bovine milk whey protein, unpurified goat milk whey protein, unpurified ovine milk whey protein or Unpurified human milk whey protein.

Detailed ways

For the convenience of further description, the following examples will be cited for more specific description.

The following examples are specific illustrations of the present invention, but do not limit the scope of the present invention thereby.

Example 1

Take bovine lactoferrin (50 grams), chromium trichloride hexahydrate (5.125 grams), add pure water (500 milliliters), heat to about 72 ° C and stir and mix for two hours, cool to room temperature, filter impurities to obtain a mixed solution of organic chromium The obtained organic chromium mixture is spray-dried to obtain the trivalent chromium composite powder of the present invention.

Example 2

Take bovine lactoferrin (30g), bovine whey protein (200g), chromium trichloride hexahydrate (5.125g), add pure water (1,000ml), heat to about 70°C and stir for two hours, cool to room temperature, The mixed liquid of organic chromium is obtained by filtering the impurities, and the obtained organic chromium is mixed and spray-dried to obtain the trivalent chromium compound powder of the present invention.

Example 3

Repeat the steps of Example 1, but mix the obtained organic chromium mixture with 100 kg of milk powder by spray drying to obtain a trivalent chromium milk product.

Example 4

Repeat the steps of Example 2, but mix the obtained organic chromium mixture with 100 kg of milk powder by spray drying to obtain a trivalent chromium milk product.

Example 5

Repeat the steps of Example 1, but add the obtained organic chromium mixture into 900 kg of fresh milk to obtain trivalent chromium fresh milk products.

Test example 1

The trivalent chromium bovine milk product obtained in Example 3 is mixed into the mouse diet (Lab Diet, 5001 Rodent DMI Nutrition International, Inc, MO, USA), and the trivalent chromium 400ppb of the trivalent chromium bovine milk product is added to the experimental group , the control group did not add trivalent chromium milk products, fed 12-week-old male congenital type II diabetic mice KK/H1J, after two weeks of free food, fasted for 12 hours, and fed glucose (1g/ kg body weight) to carry out the glucose tolerance test, measure the blood glucose (mg/dl) after 0, 2, and 4 hours respectively, and the results are shown in Table 1. As mentioned above, the blood glucose of mice fed with trivalent chromium cow milk products was significantly lower than that of control mice without trivalent chromium cow milk products (P<0.01) two hours after glucose feeding.

Table 1. Type 2 diabetic mice KK/H1J fed cow milk products containing trivalent chromium

Glucose tolerance test after two weeks of feeding. no 0hr 2 hours 4 hours test group 10 95±8 208±9 ^** 124±8 control group 8 95±8 263±14 ^** 144±9

Test example 2

With the trivalent chromium cow's milk product made in embodiment 3, mix into mouse diet (Lab Diet, 5001 Rodent DMI Nutrition International, Inc, MO, USA), the experimental group adds trivalent chromium 200ppb of trivalent chromium cow's milk product , the control group was fed 7-week-old female C57KLKS/J-lepr ^db m congenitally obese type 2 diabetic mice without adding trivalent chromium milk products. Fasting blood glucose (mg/dl), the results are as described in table 2. Its non-fasting blood glucose of mice fed trivalent chromium cow milk products is significantly lower than the control group mice without adding trivalent chromium cow milk products (P <0.05).

Table 2. C57KLKS/J-lepr ^db m feeding of congenitally obese type II diabetic mice

A four-month experiment with diets containing trivalent chromium dairy products. no Non-fasting blood glucose at trial start non-fasting blood glucose after the test test group 5 489±18 434±18 ^* mg/dl control group 5 470±20 514±20 ^* mg/dl

Test example 3.

The trivalent chromium bovine milk product obtained in Example 4 is mixed into the mouse diet (Lab Diet, 5001 Rodent DMI Nutrition International, Inc, MO, USA), and the trivalent chromium 400ppb of the trivalent chromium bovine milk product is added to the experimental group , the control group did not add trivalent chromium cow milk products, fed 8-week-old male ICR mice induced by STZ (streptozotocin) drugs to induce type 1 diabetes, and after four weeks of free food, the non-fasting blood glucose of the experimental group and the control group (mg/dl), the result is as described in table 3, its non-fasting blood glucose of the STZ diabetic mouse that feeds trivalent chromium cow milk product is significantly lower than the control group STZ diabetic mouse that does not add trivalent chromium cow milk product ( P<0.05).

Table 3. Feeding of male ICR mice induced with type 1 diabetes by STZ drugs

Four-week experiment with diets containing trivalent chromium dairy products. no Non-fasting blood glucose at trial start non-fasting blood glucose after the test test group 8 453±24 364±21 ^* mg/dl control group 8 454±24 422±21 ^* mg/dl

The trivalent chromium milk product of the present invention is a kind of food or health food. The trivalent chromium dairy product of the present invention can be eaten or taken by diabetics, and can effectively reduce their blood sugar, blood fat and cholesterol.

What have been described above are several preferred embodiments, but all partial alterations or changes originating from the technical idea of this case and easily deduced by those skilled in the art do not depart from the scope of the patent right of the present invention.

Claims (1)

1. The application of the trivalent chromium complex in the preparation of preparations for treating diabetes, the trivalent chromium complex is formed by adding the trivalent chromium compound to lactoferrin or whey protein and heat-treating it at 60°C to 80°C The trivalent chromium complex, the trivalent chromium compound is selected from chromium trichloride hexahydrate, chromium trichloride, chromium acetate, chromium nitrate, chromium oxide, or chromium sulfate, and the lactoferrin or whey protein is selected from Bovine lactoferrin, goat lactoferrin, sheep lactoferrin, human lactoferrin, unpurified cow milk whey protein, unpurified goat milk whey protein, unpurified sheep milk whey protein or unpurified Purified human milk whey protein.