CN118303531A - Additive composition and application thereof - Google Patents

Abstract

The invention provides an additive composition and application thereof, wherein the additive composition comprises, by mass, 0.6-0.9 part of vitamin A, 10-20 parts of calcium pantothenate, 2-5 parts of folic acid, 100-800 parts of vitamin C, 13.36-26.72 parts of vitamin E, 15-30 parts of vitamin K ₃ and 8000-12000 parts of isatis root polysaccharide. The additive composition can be used for remarkably improving the immunity of the Acipenser sinensis in the feeding process of the Acipenser sinensis, effectively improving the cure rate of the skin diseases of the Acipenser sinensis and promoting the large-scale cultivation of the Acipenser sinensis.

Description

Additive composition and application thereof

Technical Field

The invention relates to an additive composition and application thereof, belonging to the field of biotechnology.

Background technique

The Chinese sturgeon is a large river and sea migratory fish unique to my country and is listed as a national first-class protected aquatic animal. Due to the influence of various factors, its natural population resources have declined sharply, and artificial seed conservation has become an important way to protect this species. With the expansion of the scale of ex situ protection of the Chinese sturgeon and the continuous improvement of the degree of intensiveness, disease problems are also increasing. In recent years, a new type of skin disease has appeared in the breeding process of Chinese sturgeon, with the main symptoms of small red pustules on the body surface and red blood around the pustules, which has a certain impact on the healthy breeding of Chinese sturgeon. At present, conventional drugs and usage methods are not effective in preventing and treating this disease.

Therefore, how to improve the immunity of Chinese sturgeon and the cure rate of Chinese sturgeon skin diseases and promote large-scale breeding of Chinese sturgeon has become an urgent problem to be solved in this field.

Summary of the invention

The invention provides an additive composition. After being used in Chinese sturgeon breeding, the additive composition can significantly improve the immunity of Chinese sturgeon and improve the cure rate of Chinese sturgeon skin diseases.

The present invention also provides a feed, which comprises the additive composition, thereby being able to significantly improve the cure rate of skin diseases of Chinese sturgeon and promote large-scale breeding of Chinese sturgeon.

The present invention also provides a method for breeding Chinese sturgeon. The above-mentioned feed is used in the breeding process, so the cure rate of skin diseases of Chinese sturgeon can be significantly improved, and the large-scale breeding of Chinese sturgeon can be promoted.

The present invention also provides an application of the additive composition as a medicine for preparing a medicine for treating a skin disease of a Chinese sturgeon. The additive composition is used in the process of preparing a medicine for treating a skin disease of a Chinese sturgeon, and has a significant effect on treating the skin disease of a Chinese sturgeon.

The present invention also provides a pharmaceutical composition, which includes the above-mentioned additive composition, so the pharmaceutical composition can effectively treat the skin disease of Chinese sturgeon and promote the large-scale breeding of Chinese sturgeon.

In one aspect, the present invention provides an additive composition, which comprises, by weight, 0.6-0.9 parts of vitamin A, 10-20 parts of calcium pantothenate, 2-5 parts of folic acid, 100-800 parts of vitamin C, 13.36-26.72 parts of vitamin E, 15-30 parts of vitamin K ₃ , and 8000-12000 parts of Radix Isatidis polysaccharide.

The additive composition as described above comprises, by weight, 0.8 parts of vitamin A, 16 parts of calcium pantothenate, 4 parts of folic acid, 600 parts of vitamin C, 20.04 parts of vitamin E, 24 parts of vitamin K ₃ , and 10,000 parts of Radix Isatidis polysaccharide.

In another aspect, the present invention provides a feed comprising the additive composition as described above.

The feed as described above also includes conventional feed, and the mass ratio of the additive composition to the conventional feed is 1.1:100.

In another aspect, the present invention provides a method for raising Chinese sturgeon, which comprises raising Chinese sturgeon using the feed as described above.

In the feeding method as described above, the daily feeding amount during the feeding process is 1.0%-1.3%, the daily feeding frequency is 2 times/day, and the feeding interval is 12 hours.

In another aspect, the present invention provides a use of the additive composition as described above in preparing a medicament for treating skin diseases of Chinese sturgeon.

In another aspect, the present invention provides a pharmaceutical composition comprising the additive composition as described above.

The pharmaceutical composition as described above further comprises a first auxiliary agent, which comprises, by weight, 5-20 parts of fluoroquinolone, 0.2 parts of adenosine triphosphate disodium, 0.5 parts of coenzyme A, and 100 parts of compound amino acids.

The pharmaceutical composition as described above further comprises a second auxiliary agent, which comprises, by weight, 1 part of lithospermum oil, 2 parts of erythromycin ointment, and 1 part of Yunnan Baiyao.

The additive composition provided by the present invention is composed of raw materials of specific components. Under the synergistic effect of the raw materials of the specific components, it helps to promote the absorption and utilization of protein by the Chinese sturgeon, promote the development and regeneration of epidermal cells, promote blood coagulation, improve the immune ability of the Chinese sturgeon, effectively control infection, enhance the anti-stress, anti-inflammatory and antioxidant capabilities of the Chinese sturgeon, effectively prevent and control Chinese sturgeon skin diseases, and play an important role in protecting the Chinese sturgeon species.

The feed provided by the present invention includes the above-mentioned additives, and thus can significantly improve the immunity of Chinese sturgeons and increase the cure rate of Chinese sturgeon skin diseases.

The feeding method provided by the present invention uses the above feed, which is helpful to enhance the immunity of Chinese sturgeon and enhance the ability of Chinese sturgeon to resist skin diseases.

The present invention provides the use of the additive composition as a drug for preparing a drug for treating a Chinese sturgeon skin disease. In the process of preparing a drug for treating a Chinese sturgeon skin disease, the additive composition is used, which has a significant effect on treating the Chinese sturgeon skin disease.

The pharmaceutical composition provided by the present invention comprises the above-mentioned additive composition, so the pharmaceutical composition can effectively treat the skin disease of Chinese sturgeon and promote the large-scale breeding of Chinese sturgeon.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a partial photo of a Chinese sturgeon infected with skin disease in the present invention;

FIG2 is a photograph of pathogenic microorganism separation in one specific embodiment of the present invention;

FIG3 is a Gram staining diagram of pathogenic microorganisms in a specific embodiment of the present invention;

FIG. 4 is a diagram showing electrophoresis detection of PCR products in a specific embodiment of the present invention.

Detailed ways

In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution in the embodiment of the present invention will be clearly and completely described below in combination with the embodiment of the present invention. Obviously, the described embodiment is a part of the embodiment of the present invention, not all the embodiments. Based on the embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

In one aspect, the present invention provides an additive composition, which comprises, by weight, 0.6-0.9 parts of vitamin A, 10-20 parts of calcium pantothenate, 2-5 parts of folic acid, 100-800 parts of vitamin C, 13.36-26.72 parts of vitamin E, 15-30 parts of vitamin K ₃ , and 8000-12000 parts of Radix Isatidis polysaccharide.

The additive composition provided by the invention consists of raw materials of specific components and can be used for preventing and treating skin diseases of Chinese sturgeon.

The present invention does not limit the method for determining the pathogenic microorganisms of the skin disease of Chinese sturgeon, and the diseased Chinese sturgeon can be diagnosed by the common diagnostic methods in the art.

For example, when red pustules appear at the base of the pectoral fin, dorsal fin, anal fin or other epidermal parts of the Chinese sturgeon, and the pustules are surrounded by red blood streaks, as shown in Figure 1, a sterile syringe is used to extract 1 mL of blood from the red pustules of the diseased fish, and inoculated to the BHIA plate under sterile conditions, cultured for 24-48 hours, and typical dominant colonies are picked from the initial culture plate and transferred to the BHIA plate for purification culture. After 24 hours of purification culture, the morphological characteristics of the colonies are observed, as shown in Figure 2, showing a round, smooth surface, opaque, milky white color, as shown in Figure 3, and the Gram staining results show Gram-positive bacteria. Subsequently, the purified strains are inoculated into BHI liquid culture medium, cultured at 28°C for 16-24 hours, and the 16srDNA detection technology is used to obtain the electrophoresis detection PCR product map, as shown in Figure 4, to determine the pathogenic microorganism.

The vitamin A in the present invention can protect the health and integrity of epithelial tissue, promote the development and regeneration of mucous membranes and skin; promote the synthesis of polysaccharides in connective tissue, maintain the integrity of cell membranes and organelle membrane structures; maintain the normal growth and repair of bones; maintain the normal function of nerve cells; and improve the body's immune ability.

Calcium pantothenate, also known as vitamin B ₃ , can participate in fatty acid synthesis and degradation, citric acid cycle, choline acetylation and antibody synthesis, and promote the utilization of nutrients.

Folic acid is vitamin _B11 , which is converted into tetrahydrofolic acid by vitamin C in the body. Tetrahydrofolic acid is a coenzyme of carbonyl transferase, which affects the synthesis of nucleic acids and promotes the formation of proteins and new cells.

Vitamin C participates in the body's redox process, affecting the formation of nucleic acids, iron absorption, hematopoiesis, detoxification and immune function. It mainly affects the nonspecific immunity of aquatic animals, promotes the formation of antibodies, and thus improves immunity.

Vitamin E is a physiological antioxidant in the body that can reduce tissue oxygen consumption, enhance mitochondrial function, affect nucleic acid metabolism and the functions of various enzymes; it has a nonspecific detoxification effect, prolongs cell survival, degrades lipids, and helps trauma recovery.

Vitamin _K3 is an anticoagulant factor that promotes the formation of prothrombin and maintains normal coagulation time; it also has the effect of strengthening the liver's detoxification function.

Radix Isatidis polysaccharide is the main chemical component in Radix Isatidis and the main factor of biological activity. It has antibacterial, antioxidant, antiviral, anti-inflammatory, and immune-enhancing effects.

When the above components are mixed according to the above mass parts, they have a good effect on the skin disease of Chinese sturgeon. The inventors analyzed that the reason may be that the combination of various components in specific mass parts can give full play to the synergistic effect between the components, promote the absorption and utilization of protein by Chinese sturgeon, promote the development and regeneration of epidermal cells, promote blood coagulation, improve the immune ability of Chinese sturgeon, effectively control infection, enhance the anti-stress, anti-inflammatory and antioxidant capabilities of Chinese sturgeon, and improve the cure rate of skin disease of Chinese sturgeon.

Furthermore, in a specific embodiment of the present invention, the additive composition includes, by mass, 0.8 parts of vitamin A, 16 parts of calcium pantothenate, 4 parts of folic acid, 600 parts of vitamin C, 20.04 parts of vitamin E, 24 parts of vitamin K ₃ , and 10,000 parts of Radix Isatidis polysaccharide.

When the various components of the additive composition meet the above mass parts, the prepared additive has a higher cure rate when used to feed Chinese sturgeons infected with skin diseases. The reasons may be: (1) The synergistic effect of the additive combination: the ratio of multiple vitamins is reasonable, and the synergistic effect is enhanced. First, the anti-stress ability of the Chinese sturgeon is enhanced; second, blood coagulation and the development and regeneration of epidermal cells are promoted, which has a good effect on the recovery of bacterial diseases; third, the settlement of pathogenic bacteria is inhibited, and the development of the disease is controlled; fourth, appetite is enhanced, and the absorption and utilization of protein by the Chinese sturgeon is promoted. The combination of Radix Isatidis polysaccharides and multiple vitamins can effectively improve the immunity of the Chinese sturgeon, enhance the anti-stress, anti-inflammatory and antioxidant abilities, and thus achieve a good prevention and treatment effect. (2) The dosage of the additive combination: the dosage of the additive combination is within the appropriate safe dosage range, which effectively plays a role in improving the immunity of the Chinese sturgeon, reducing the invasion of pathogenic microorganisms, and promoting the recovery of the disease. During use, there will be no poor disease prevention and treatment effect due to insufficient dosage, and there will be no adverse effects on the Chinese sturgeon due to excessive dosage.

In another aspect, the present invention provides a feed comprising the additive composition as described above.

Since the feed provided by the present invention includes the additive composition, when used for feeding Chinese sturgeons infected with skin diseases, the immunity of Chinese sturgeons can be significantly improved, and the cure rate of infected Chinese sturgeons can be increased.

The feed as described above also includes conventional feed, and the mass ratio of the additive composition to the conventional feed is 1.1:100.

The present invention does not limit the nutritional components of conventional feeds, and the Chinese sturgeon feed commonly used in the art can be selected.

In a specific embodiment, the conventional feed comprises crude protein ≥44.0wt%, crude fat ≥10.0wt%, crude fiber ≤5.0wt%, crude ash ≤16.0wt%, total phosphorus ≥1.2wt%, and lysine ≥2.5wt%.

When the mass ratio of the conventional feed to the additive composition is within the above range, it can not only effectively control the feeding cost but also has an excellent effect of treating the skin disease of the Chinese sturgeon.

In another aspect, the present invention provides a method for raising Chinese sturgeon, which comprises raising Chinese sturgeon using the feed as described above.

Since the Chinese sturgeon is raised with the above feed, the raising method provided by the present invention can effectively treat the skin disease of the Chinese sturgeon.

Furthermore, in a specific embodiment of the present invention, the daily feeding amount during the feeding process is 1.0%-1.3% of the body weight, the daily feeding frequency is 2 times/day, and the feeding interval is 12 hours.

The daily feeding amount in the present invention refers to the ratio of the mass of feed fed every day to the average mass of Chinese sturgeon.

The daily feeding amount is 1.0%-1.3% of body weight. For example, the daily feeding amount includes but is not limited to 1.0%, 1.1%, 1.2%, 1.3% or a range consisting of any two of them.

In a specific embodiment, the feeding is carried out at 7:00 every day, and the feeding amount is 0.5%-0.65% of the body weight; the feeding is carried out at 19:00 every day, and the feeding amount is 0.5%-0.65% of the body weight.

It is understandable that the feeding amount and feeding time interval can be adjusted within the above range according to the feeding situation of the Chinese sturgeon in the breeding device.

When the Chinese sturgeon is fed according to the above feeding method, not only can the Chinese sturgeon's intake balance be maintained, so that the Chinese sturgeon can better digest and absorb the feed and additive composition, but also the additive composition can fully exert its effect and improve the cure rate of the Chinese sturgeon.

In another aspect, the present invention provides a use of the additive composition as described above in preparing a medicament for treating skin diseases of Chinese sturgeon.

In detail, the additive composition is used in the process of preparing a drug for treating skin diseases of Chinese sturgeon.

The present invention does not limit the specific preparation process of the drug for treating skin diseases of Chinese sturgeon, and a common preparation method in the art can be selected.

In another aspect, the present invention provides a pharmaceutical composition comprising the additive composition as described above.

Since the pharmaceutical composition provided by the present invention includes the above-mentioned additive composition, it has a significant effect in the process of treating skin diseases of Chinese sturgeon.

Furthermore, in a specific embodiment of the present invention, the pharmaceutical composition as described above further comprises a first auxiliary agent, which comprises 5-20 parts of fluoroquinolone, 0.2 parts of adenosine triphosphate disodium, 0.5 parts of coenzyme A, and 100 parts of compound amino acids in parts by mass.

The first auxiliary drug in the present invention is an injectable drug.

In detail, the first auxiliary agent includes 5-20 parts of fluoroquinolone by mass. For example, the mass of fluoroquinolone includes but is not limited to 5 parts, 10 parts, 15 parts, 20 parts or any two parts thereof.

It is understandable that the first auxiliary agent also contains physiological saline.

In a specific embodiment, the first auxiliary agent includes 5-20 parts of fluoroquinolone, 0.2 parts of adenosine triphosphate disodium, 0.5 parts of coenzyme A, and 100 parts of compound amino acids in parts by mass.

The present invention does not limit the preparation process of the first auxiliary agent. In a specific embodiment, first, 1 mL of normal saline is used to dissolve adenosine triphosphate disodium, and 1 mL of normal saline is used to dissolve coenzyme A. Subsequently, a syringe is used to draw the dissolved adenosine triphosphate disodium and coenzyme A, and then fluoroquinolone and compound amino acids are drawn in sequence to obtain the first auxiliary agent.

In order to ensure the effectiveness of the first auxiliary agent, the first auxiliary agent needs to be prepared and used immediately.

The fluoroquinolone in the present invention is a broad-spectrum antibiotic specially used for animals, and is mainly used to prevent and treat bacterial diseases such as Aeromonas disease, Pseudomonas disease, Vibrio disease, Flexibacterium disease, Streptococcus disease, Pasteurella disease, Nocardia disease, Edwardsiella disease, and mycobacterial disease in fish.

The adenosine triphosphate disodium in the present invention is a coenzyme drug that participates in the metabolism of fat, protein, sugar, nucleic acid and nucleotide in the body and plays an important role in life activities. It can provide energy and restore physical fitness when used in combination with coenzyme A.

In the present invention, coenzyme A is an energy nutrient, a coenzyme of acetylation reaction in the body, participates in acetylation reaction in the body, and plays an important role in the metabolism of sugar, fat and protein.

The compound amino acid (18AA) in the present invention is a nutritional supplement having the effects and functions of nutritional support, enhancing immune function, repairing tissue damage, promoting tissue healing and the like.

When using the first auxiliary agent of the present invention, the dosage of the first auxiliary agent can be adjusted according to the weight and disease severity of the Chinese sturgeon. For example, in one specific embodiment, the mass ratio of the first auxiliary agent to the Chinese sturgeon is 1:10000.

In a specific embodiment, the method for using the first auxiliary agent in the present invention is as follows: place the Chinese sturgeon infected with skin disease in a treatment box, use 60-80ppm MS-222 to anesthetize the diseased fish, weigh the diseased fish after anesthesia, and then inject the first auxiliary agent into the Chinese sturgeon through the base of the pectoral fin according to the mass ratio of the first auxiliary agent to the infected Chinese sturgeon of 1:10000, once a day, for 5-8 consecutive days as a course of treatment, and 1-2 courses of injection.

When the pharmaceutical composition includes the additive composition and the above-mentioned first auxiliary agent, it can effectively inhibit bacterial infection, promote skin tissue repair and regeneration, provide energy and nutrients required by cells, and further improve the cure rate of Chinese sturgeon skin diseases.

Furthermore, in a specific embodiment of the present invention, the pharmaceutical composition also includes a second auxiliary agent, which includes 1 part of lithospermum oil, 2 parts of erythromycin ointment, and 1 part of Yunnan Baiyao in parts by mass.

The second auxiliary agent of the present invention is used as an external agent.

The lithospermum oil of the present invention has multiple functions such as antibacterial and anti-inflammatory, analgesic, heat-clearing and blood-cooling, hemostasis, etc., has inhibitory effects on cocci, Escherichia coli, and various fungi, and has significant therapeutic effects on healing of skin wounds on the body surface.

The erythromycin ointment of the present invention is a macrolide external antibacterial drug, which can kill a variety of gram-positive bacteria and gram-negative bacteria, and is mainly used for purulent skin diseases such as pustule, small-area burns, ulcer surface infections and acne vulgaris.

The Yunnan Baiyao in the present invention has the effects of promoting blood circulation and removing blood stasis, stopping bleeding, reducing swelling, reducing inflammation, promoting wound healing, etc., and can treat traumatic injuries, oral ulcers, skin abscesses, etc.

The second auxiliary agent in the present invention only needs to be prepared by mixing 1 part of lithospermum oil, 2 parts of erythromycin ointment and 1 part of Yunnan Baiyao into a paste-like agent.

The present invention does not limit the specific use method of the second auxiliary agent. In a specific embodiment, the second auxiliary agent is evenly applied on the surface of the lesion, once a day, for 5-8 consecutive days as a course of treatment, and 1-2 courses of external use.

It is understandable that the use process and dosage of the second auxiliary agent can be appropriately adjusted according to the degree of skin disease infection in the Chinese sturgeon.

In order to make the second auxiliary agent fully play its role, the infected part of the Chinese sturgeon can be treated before using the second auxiliary agent. In a specific embodiment, the lesion on the surface of the Chinese sturgeon is disinfected with iodine or medical alcohol cotton balls, and then the blood is sucked out with a sterile syringe.

The synergistic effect of the additive composition and the first auxiliary agent and the second auxiliary agent provides a more comprehensive treatment, including reducing inflammation, inhibiting infection, and promoting the repair and healing of skin tissue, which plays an important role in improving the cure rate of Chinese sturgeon skin diseases.

The additive composition provided by the present invention is further described below in conjunction with specific examples.

The Chinese sturgeon in the present invention comes from the Yichang Experimental Station of the Chinese Sturgeon Research Institute.

Example 1

The preparation methods of the additive composition, the first auxiliary agent, and the second auxiliary agent of this embodiment are as follows:

1) Preparation of additive composition: weigh 0.78 mg vitamin A (0.72 parts), 15.24 mg calcium pantothenate (14 parts), 3.81 mg folic acid (3.5 parts), 598.72 mg vitamin C (550 parts), 18.18 mg vitamin E (16.70 parts), 21.77 mg vitamin _K3 (20 parts), 10.34 g Radix Isatidis polysaccharide (9500 parts), and mix the above components evenly to prepare an additive composition.

2) Preparation of the first auxiliary agent: First, dissolve 1.7 mg of adenosine triphosphate disodium (2 parts) and 4.1 mg of coenzyme A (5 parts) in 1 ml of normal saline, then use a syringe to draw out the dissolved adenosine triphosphate disodium and coenzyme A, and then draw out 165.7 mg of fluoroquinolone (200 parts) and 828.5 mg of compound amino acids (1000 parts) in turn to mix the first auxiliary agent.

3) Preparation of the second auxiliary agent: weigh 1g of lithospermum oil, 2g of erythromycin ointment, and 1g of Yunnan Baiyao, and mix the three drugs evenly to prepare the second auxiliary agent.

The above-mentioned additive combination group, the first auxiliary agent and the second auxiliary agent constitute a pharmaceutical composition.

Example 2

The preparation methods of the additive composition, the first auxiliary agent, and the second auxiliary agent of this embodiment are as follows:

1) Preparation of additive composition: weigh 0.86 mg vitamin A (0.88 parts), 17.64 mg calcium pantothenate (18 parts), 4.41 mg folic acid (4.5 parts), 636.98 mg vitamin C (650 parts), 22.91 mg vitamin E (23.38 parts), 27.44 mg vitamin _K3 (28 parts), 10.29 g Radix Isatidis polysaccharide (10500 parts), and mix the above components uniformly to prepare an additive composition.

2) Preparation of the first auxiliary agent: First, dissolve 1.9 mg of adenosine triphosphate disodium (2 parts) and 4.7 mg of coenzyme A (5 parts) in 1 ml of normal saline, then use a syringe to draw the dissolved adenosine triphosphate disodium and coenzyme A, and then draw 47 mg of fluoroquinolone (50 parts) and 950 mg of compound amino acids (1000 parts) in turn to mix into the first auxiliary agent.

3) Preparation of the second auxiliary agent: weigh 1g of lithospermum oil, 2g of erythromycin ointment, and 1g of Yunnan Baiyao, and mix the three drugs evenly to prepare the second auxiliary agent.

The above-mentioned additive combination group, the first auxiliary agent and the second auxiliary agent constitute a pharmaceutical composition.

Example 3

The preparation methods of the first auxiliary agent and the second auxiliary agent of this embodiment are the same as those of Embodiment 1, but the preparation method of the additive composition is different, which is specifically as follows:

In step 1), 0.81 mg vitamin A (0.6 parts), 13.5 mg calcium pantothenate (10 parts), 2.7 mg folic acid (2 parts), 135.12 mg vitamin C (100 parts), 18.05 mg vitamin E (13.36 parts), 20.27 mg vitamin _K3 (15 parts), and 10.81 g isatis polysaccharide (8000 parts) were weighed and uniformly mixed to prepare an additive composition.

The above-mentioned additive combination group, the first auxiliary agent and the second auxiliary agent constitute a pharmaceutical composition.

Example 4

The preparation methods of the first auxiliary agent and the second auxiliary agent of this embodiment are the same as those of Embodiment 1, but the preparation method of the additive composition is different, which is specifically as follows:

In step 1), 0.77 mg vitamin A (0.9 parts), 17.08 mg calcium pantothenate (20 parts), 4.27 mg folic acid (5 parts), 683.09 mg vitamin C (800 parts), 22.82 mg vitamin E (26.72 parts), 25.62 mg vitamin _K3 (30 parts), and 10.25 g Radix Isatidis polysaccharide (12000 parts) were weighed and uniformly mixed to prepare an additive composition.

The above-mentioned additive combination group, the first auxiliary agent and the second auxiliary agent constitute a pharmaceutical composition.

Example 5

The preparation methods of the first auxiliary agent and the second auxiliary agent of this embodiment are the same as those of Embodiment 1, but the preparation method of the additive composition is different, which is specifically as follows:

In step 1), 0.82 mg vitamin A (0.8 parts), 16.50 mg calcium pantothenate (16 parts), 4.13 mg folic acid (4 parts), 618.86 mg vitamin C (600 parts), 20.67 mg vitamin E (20.04 parts), 24.75 mg vitamin _K3 (24 parts), and 10.31 g Radix Isatidis polysaccharide (10000) were weighed and uniformly mixed to prepare an additive composition.

The above-mentioned additive combination group, the first auxiliary agent and the second auxiliary agent constitute a pharmaceutical composition.

Example 6

The preparation methods of the additive composition and the first auxiliary agent in this embodiment are the same as those in Example 1, except that the second auxiliary agent is not included in the pharmaceutical composition in this embodiment.

Example 7

The preparation methods of the additive composition and the second auxiliary agent in this embodiment are the same as those in Example 1, except that the first auxiliary agent is not included in the pharmaceutical composition in this embodiment.

Comparative Example 1

The preparation method of the first auxiliary agent and the second auxiliary agent in this comparative example is the same as that in Example 1, except that the drug composition in this comparative example does not include an additive composition.

The first auxiliary drug and the second auxiliary drug constitute a pharmaceutical composition.

Comparative Example 2

The preparation methods of the first auxiliary agent and the second auxiliary agent in this comparative example are the same as those in Example 1, but the preparation method of the additive composition is different, as follows:

In step 1), 9.6 mg vitamin A (9 parts), 106.7 mg calcium pantothenate (100 parts), 21.33 mg folic acid (20 parts), 10.67 g vitamin C (10,000 parts), 106.67 mg vitamin E (100 parts), 3.2 mg vitamin K ₃ (3 parts), and 85.33 mg Radix Isatidis polysaccharide (80 parts) were weighed and uniformly mixed to prepare an additive composition.

Test Case

The additive composition prepared in the above Examples 1-5 and Comparative Example 2 was mixed with conventional feed at a mass ratio of 1.1:100 to prepare feed.

During 2020-2022, the Yichang Experimental Station of the Chinese Sturgeon Research Institute developed fish diseases in the second generation of Chinese sturgeons aged 1-2 years. The following method was used to diagnose and treat the fish diseases. When the drug composition did not contain at least one of the additive composition, the first auxiliary agent and the second auxiliary agent, the agent or composition was not used for treatment.

(1) Diagnosis of skin diseases: After discovering the diseased fish, check the diseased fish. Small red pustules will appear at the base of the pectoral fin, dorsal fin, anal fin or other epidermal parts of the diseased fish, and the pustules will be surrounded by red blood streaks.

(2) Treatment of sick fish: Pick up the sick fish and place them in a treatment box. The operation of picking up the sick fish needs to be done gently and with water. Use 60-80ppm MS-222 to anesthetize the sick fish. After the sick fish is anesthetized, weigh the sick fish. Inject the first auxiliary agent into the body of the Chinese sturgeon through the base of the pectoral fin at a mass ratio of 1:10000. Inject once a day for 5-8 consecutive days as a course of treatment, and inject 1-2 courses of treatment. Use iodine tincture or medical alcohol cotton balls to disinfect the lesions on the surface of the Chinese sturgeon, and then use a sterile syringe to absorb the blood. Apply the second auxiliary agent evenly on the surface of the lesions. Apply once a day for 5-8 consecutive days as a course of treatment, and use 1-2 courses of treatment.

(3) Feeding of sick fish: Feed sick fish according to the feeding rule of 1.0%-1.3% of body weight per day, 2 times per day, and 12 hours interval between feedings.

The therapeutic effects of the pharmaceutical compositions provided in various embodiments and comparative examples are statistically analyzed, as shown in Table 1.

Table 1

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims (10)

1. The additive composition is characterized by comprising, by mass, 0.6-0.9 part of vitamin A, 10-20 parts of calcium pantothenate, 2-5 parts of folic acid, 100-800 parts of vitamin C, 13.36-26.72 parts of vitamin E, 15-30 parts of vitamin K ₃ and 8000-12000 parts of isatis root polysaccharide.

2. The additive composition according to claim 1, wherein the additive composition comprises, in parts by mass, 0.8 part of vitamin a, 16 parts of calcium pantothenate, 4 parts of folic acid, 600 parts of vitamin C, 20.04 parts of vitamin E, 24 parts of vitamin K ₃, 10000 parts of isatis root polysaccharide.

3. A feed comprising the additive composition of claim 1 or 2.

4. A feed according to claim 3, further comprising a conventional feed, the mass ratio of the additive composition to the conventional feed being 1.1:100.

5. A method for raising Acipenser sinensis, characterized in that the feed of claim 3 or 4 is used for raising Acipenser sinensis.

6. The feeding method according to claim 5, wherein the daily feeding amount is 1.0% -1.3%, the daily feeding frequency is 2 times/day, and the feeding time interval is 12 hours.

7. Use of an additive composition according to claim 1 or 2 for the preparation of a medicament for the treatment of skin disorders of Acipenser sinensis.

8. A pharmaceutical composition comprising the additive composition of claim 1 or 2.

9. The pharmaceutical composition of claim 8, further comprising a first auxiliary agent comprising 5-20 parts by mass of fiuorothiamphenicol, 0.2 part by mass of disodium adenosine triphosphate, 0.5 part by mass of coenzyme a,100 parts by mass of compound amino acid.

10. The pharmaceutical composition according to claim 9, further comprising a second auxiliary agent comprising 1 part of arnebia oil, 2 parts of erythromycin ointment, 1 part of yunnan white drug in parts by mass.