CN1171539C - Nutrients additive for biological feed of livestock and fowls and its preparing process - Google Patents
Nutrients additive for biological feed of livestock and fowls and its preparing process Download PDFInfo
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- CN1171539C CN1171539C CNB021156115A CN02115611A CN1171539C CN 1171539 C CN1171539 C CN 1171539C CN B021156115 A CNB021156115 A CN B021156115A CN 02115611 A CN02115611 A CN 02115611A CN 1171539 C CN1171539 C CN 1171539C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention belongs to a nutrient additive of poultry and fowl microbe feed, which is prepared from microbes of fermentation products of lysine strains, bacillus, photosynthetic bacteria, etc., bean pulp, plant starch and trace elements. The additive uses amino acid raw liquor by microbe fermentation to keep a complete amino acid pattern, and thereby, the biological value of animals is high, harmful substances of ammoniacal nitrogen, nitrous acid, hydrogen sulfide, etc. in vivo of poultry and fowls are decomposed, the smell of feces is reduced, and the pollution of excrements of poultry and fowls to the environment is decreased. Meanwhile, the additive is a high-grade strain high protein and contains abundant methionine, so the additive has special effect on improving the immunity of animals and better effect on enhancing the utilization rate of proteins, improving the growth condition of poultry and fowls, increasing the immunological functions of the poultry and the fowls, etc. than the traditional similar feed.
Description
Technical field
The present invention relates to a kind of animal and fowl fodder nourishing additive agent, particularly livestock and poultry nutrients additive for biological feed and the production method made from the product of microbial fermentation thereof.
Background technology
In order to improve raising quality and the speed of livestock and poultry, people are more and more deep for the research of feed, quality requirement to animal product is more and more higher, the security of animal product is subjected to paying attention to widely, and the feed addictive emphasis that to be people study, therefore biological feedstuff product pollution-free, nuisanceless, noresidue is favourably welcome.Existing feed addictive mainly contains trace element and amino acids, and amino acid feed additive is biological feedstuff again, this production technology by people be called the 4th generation feed technology.The application of biotechnology in feed industry at present has: six big classes such as microbial fermentation goods, feed enzyme preparation, functional microorganism preparation, biologically active peptide, green bio feed, immunomodulator or immunopotentiator.These biological feedstuffs, biology feed additive are all brought into play distinctive effect separately, but along with country and common people are strengthening and raising the concern dynamics of feed safety problem and degree, biological feedstuff should also have bigger research and development space, this makes people drop into more interest in this respect, is the focus in the current feed industry.
In addition, because people are increasing to amino acid whose demand, each seed amino acid factory also develops rapidly, but it is in the amino acid whose process of industrial production, behind broth extraction amino acid, it also is alarming problem that discharging of waste liquid brings negative effect to environment, thereby the discharging of waste liquid problem that how to solve amino acid production also is the important topic that enterprise and efforts at environmental protection person will be badly in need of studying.
Summary of the invention
In order to obtain better animal and fowl fodder nourishing additive agent, improve raising quality and the speed of livestock and poultry, and the discharging of waste liquid problem that solves amino acid production as early as possible, production, the new approach of use exploitation for the amino acid industry, the inventor by the further investigation to amino acid and micro-organisms process, has manufactured experimently a kind of brand-new biological products through many tests and research, successfully use as feed supplement, obtained effect preferably.
Technical scheme of the present invention is as follows:
The livestock and poultry nutrients additive for biological feed comprises lysine bacterial classification tunning and bacillus, photosynthetic bacteria and dregs of beans, and its composition and mass content are: bacillus 1-15%, and photosynthetic bacteria 1-15%, dregs of beans 5-20%, all the other are lysine bacterial classification tunning.
More than said fermenting lysine product be all products of lysine production overall process, comprise intermediate products, finished product and their spraying, vacuum, evaporation and concentration product, dry product, the dilution product, absorbent articles, comprise the production waste liquid of intermediate products, finished product and their spraying, vacuum, evaporation and concentration product, dry product, absorbent articles with all waste liquids of lysine production overall process.
More than said bacillus comprise solid-state, semisolid, liquidly cultivate the concentrating of microbial inoculum and they, dilution, absorption, vacuum drying agent.
More than said photosynthetic bacteria be photosynthetic bacteria bacterium liquid and its concentrate, dilution, adsorbent, vacuum drying agent.
More than said dregs of beans comprise that the legume that peanut press pulp, soybean, flaxseed meal, rapeseed dregs, cottonseed cake etc. are processed into powdery comprises soy meal etc.
This additive also can add other microorganism and plant amylum and trace element except that mentioned microorganism, as saccharomycete, lactic acid bacteria.Its addition saccharomycete, lactic acid bacteria respectively account for the 1-5% of total additive quality, and trace element accounts for 0.5-1%, and plant amylum accounts for 5-15%.
More than said saccharomycete comprise solid-state, semisolid, liquidly cultivate the concentrating of microbial inoculum and they, dilution, absorption, spray-drying, vacuum drying agent.
More than said lactic acid bacteria comprise the concentrating of semisolid, the liquid microbial inoculum of cultivating and they, dilution, absorption, vacuum drying agent.
More than said trace element comprise inorganic compound, the organic compound of iron, copper, zinc, calcium, selenium, manganese, as ferrous gluconate, copper sulphate, zinc sulfate, grape calcium gluconate, manganese sulfate, sodium selenate etc.
More than said plant amylum comprise cereal starch and potato starch, as pulvis such as corn, rice, wheat, Chinese sorghum, Ipomoea batatas, cassavas, can use separately, also can be their mixture.
According to above-mentioned composition proportioning, nutrients additive for biological feed of the present invention can be liquid, or slurry, also can be the solid shape that spraying or absorption or vacuum drying obtain.
The mechanism of action of animal and fowl fodder nourishing additive agent of the present invention is as follows:
1, the biological raising poultry nutritive additive of L-lysine master medium is to obtain tens kinds of L type amino acid product based on L-lysine with lysine bacterial classification sugar fermentation honey.Lysine has the title of " special essential amino acid ", and it has outstanding effect on the utilization rate that improves protein, and lysine has been widely used in feed addictive.By-product amino acid in the lysine fermentation liquor has: aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, phenylalanine, histidine, proline.All be essential amino acid, each is comfortable improve protein utilization rate, improve aspect performance effects separately such as immunity, protein is synthetic.In addition, the many forms that can Copper lysinate of the copper in the zymotic fluid exist, and Copper lysinate can significantly improve the pig feed intake and the speed of growth of wean, and does not disturb the absorption of iron, zinc, and CuSO
4The excessive absorption that can disturb iron, zinc.
2, the bacillus hydrolyzable starch of the present invention's employing, more rare is to go back the hydrolyzable phytic acid, also produces polysaccharide material simultaneously.Staple food is corn, dregs of beans, wheat bran, jowar in livestock and poultry diet.In these legumes, though phosphorous abundant, be to exist mostly with the phytate phosphorus form, nonruminant does not have phytase can't decompose utilization with phytate phosphorus, makes that the utilization rate of plant phosphorus only is 12%～25%.The present invention make had in the animal body can decompose the bacillus of phytic acid after, can improve the utilization rate of plant phosphorus, simultaneously because its several mineral materials of having gone back chelating of phytic acid, such as calcium, iron, magnesium etc., also these mineral matters are disengaged when bacillus is decomposed phytate phosphorus, the growth that promotes animal is benefited.
3, its absorption of photosynthetic bacteria is harmful substance, and what emit is benefit materials.It can decompose harmful substances such as the ammoniacal nitrogen that produces in the livestock and poultry body, nitrous acid, hydrogen sulfide, so it can reduce the stink of ight soil, reduces the pollution of farm animal excrement to environment.
4, legume plays very important effect in the present invention, because in the legume except that containing abundant starch, protein, it still is the important source of the required mineral matter of animal, but the mineral matter in the legume, many by phytate and the strong chelating of other material, and the utilization rate of mineral matter is had a greatly reduced quality, and the chelate of legume mineral matter is in the present invention by after the probio decomposition, a large amount of free ore deposit elements are disengaged, also produce the not clear short long factor simultaneously, thereby improve its biological value greatly, promote the growth of animal.
5, lactic acid bacteria, saccharomycete all are good aid digestion probios, and still nutritious unicellular high protein of thalline itself, and they are in edible, feeding all widespread usage.
Below be the production method of animal and fowl fodder nourishing additive agent of the present invention:
The production method of livestock and poultry nutrients additive for biological feed of the present invention is by bacillus 1-15%, photosynthetic bacteria 1-15%, and dregs of beans 5-20%, all the other are the ratio of lysine bacterial classification tunning, mix, and obtain the livestock and poultry nutrients additive for biological feed.
1, L-lysine bacterial classification tunning
The fermenting lysine condition: raw material is taken from the molasses in sugar refinery, concentration: 10-30BX, adopt and produce L-lysine bacterial classification, temperature: 30～34 ℃, pressure: 0.05～0.12Mpa, air quantity: 1: 0.3～1: 1.0, PH6.0～7.5, rotating speed 250～550r.p.m.
2, bacillus
Bacillus culture medium: sucrose or molasses or glucose or starch, mass content 0.1%～1.0%, KH
2PO
40.05%～1.5%, MgSO
40.02%～0.2%, FeCl
30.0005%～0.005%, yeast extract (powder) 0.01%～0.5%, (NH
4)
2SO
40.01%～1.0%.
Bacillus condition of culture: temperature: 28～32 ℃, pressure: 0.03～0.12Mpa, air quantity: 1: 0.1～1: 1, PH5.5～7.5, rotating speed 250～550r.p.m.
3, photosynthetic bacteria
Photosynthetic bacteria culture medium: NH
4Cl0.05%～0.2%, K
2HPO
40.01%～0.5%, MgCl
20.01%～0.2%, NaCl0.05%～1.0%, yeast extract (powder) 0.005%～0.5%, NaHCO
30.01%～2.0%, ethanol 0.1%～0.5% is used H
3PO
3Transfer PH7.0
Photosynthetic bacteria condition of culture: 25～32 ℃ of illumination cultivation of anaerobism.
4, saccharomycete
Microzyme culture medium: 10 ° of BX brewer's worts, 25～30 ℃ of static culture perhaps adopt other culture medium.
5, lactic acid bacteria
Lactic acid bacteria culture medium: yeast extract 0.3%～1.0%, soy peptone 0.1%～1.0%, glucose or lactose 0.3%～2.0%, K
2HPO
40.05%～0.5%, Tomato juice 5%～10%, Tween 80 0.01%～0.1%, 35～38 ℃ of cultivation temperature, static culture.
Quick-reading flow sheets
1. lysine inclined-plane seed--shaking bottle plants--0.2m
3--5m
3--50m
3Ripe mash
2. bacillus inclined-plane kind--shaking bottle plants--0.2m
3--5m
3The ripe kind
3.--1L transparent vessel--10L transparent vessel--50L transparent vessel is ripe plants the photosynthetic bacteria seed
4. saccharomycete inclined-plane seed--1000ml triangular flask kind--0.04m
3--0.2m
3Ripe microbial inoculum
5. lactic acid bacteria inclined-plane kind--the straight cup of 500ml is planted--0.04m
3--0.2m
3Ripe microbial inoculum
6. the bran after the oil removing, the dregs of rice or legume are processed into powder, soak the back sterilization with suitable quantity of water.
With 1., 2., 3., 4., 5., 6. finished product sterile working is mixed with the biological raising poultry nutritive additive of L-lysine-pack-be up to the standards-product.
The biological raising poultry nutritive additive of L-lysine compared with similar products, the substantive distinguishing features and the obvious improvement that have are:
1, unique on the prescription
The biological raising poultry nutritive additive of L-lysine, content is the combination of fermentation liquid and probio.Molasses fermented lysine technology is the nearly 20 years continuous reservation of this research institute, perfect, raising, stable technology.Biological bacteria also is to adopt industrialized preparing process.This also is rare in China.Since we on top of microbial culture method, and the Zymolysis Equipment of multiple class is arranged, the easy to do culture technique of grasping probio, and obtain new breakthrough on technology, prescription greatly reduces the production cost of bacillus, photosynthetic bacteria.
The biological raising poultry nutritive additive of L-lysine is by lysine bacterial classification industrial fermentation molasses, produces fermenting lysine mash and probio bacillus, photosynthetic bacteria, lactic acid bacteria, the formulated biology feed additive of saccharomycete science.Such combining form does not see that in like product it is center of the present invention that report is arranged.
2, effect uniqueness, remarkable, creative
1) amino acid additive in the conventional feed is artificial interpolation, this makes the biological value of animal have limitation, and the present invention is the amino acid primary liquid with biofermentation, keep more complete amino acid pattern, can make the biological value of animal higher, improve protein utilization, improve the growth of animals or poultry situation, to strengthen aspects such as immunologic function of livestock and birds better than similar conventional feed.
2) probio among the present invention can not only hydrolyzed starch, goes back the hydrolyzable phytic acid, and this is more rare in like product.Increase this class beneficial bacterium in animal body; there is not the defective of phytase to be of great benefit to remedying nonruminant; it improves the utilization rate of phytate phosphorus; reduce the ANFs in the daily ration; directly improve the conversion ratio of feed; promote the growth of organs such as animal skeleton, also reduced ight soil row phosphorus amount simultaneously, environmental protection is of great benefit to.The present invention is meaningful greatly to the excrement phosphorus evil problem that reduces, control makes environment specialists worry.This also is of the present invention one big characteristics and intention.
3) also have photosynthetic bacteria in the probio among the present invention, it can decompose harmful substances such as the interior ammoniacal nitrogen that produces of livestock and poultry body, nitrous acid, hydrogen sulfide, reduces the stink of ight soil, reduces the pollution of farm animal excrement to environment.Therefore itself still thalline high protein of high-quality simultaneously, and contain abundant methionine have one's own knack improving on the animal immunizing power.
3, remarkable in economical benefits
After fermenting lysine product and bacillus, photosynthetic bacteria are united use, biological value height and effect is obvious is better than the lysine of single lysine fermentation liquor and even pure product.Therefore product for trial sale is popular.The production cost of fermenting lysine additive is than the pure product much less of lysine, and its price is more taller than pure product.With 100m
3Zymotic fluid by producing acid 10%, can get 7～8 tons of feed grade lysines, about about 160,000 yuan of price, and 100m
3Zymotic fluid can make 1000m
3～2000m
3The biological raising poultry nutritive additive of L-lysine, by present 0.65 yuan/L of trial sale price, price just is 65～1,300,000, but also has not had this alarming problem of discharging of waste liquid.Than directly producing feed grade lysine, each 100m
3Fermentation tank is created benefit 49～1,140,000 yuan more, calculates year many wound benefits more than 2,000 ten thousand yuan to produce 1000 tonnes of lysines per year, and is used to raise pigs, and adds this nutrient solution and compares 2 jin, a traditional unit of weight of every pig many weightening finishes every day with not adding.Obviously large-scale this series products economic benefit of production is very significant.
4, no discharging of waste liquid
The biological raising poultry nutritive additive of L-lysine all uses fermentation liquid, does not need the back extraction process, thereby does not have discharging of waste liquid.This is concerning the lysine enterprise that endures the waste water puzzlement to the fullest extent, and is meaningful greatly.
The specific embodiment
Livestock and poultry nutrients additive for biological feed composition embodiment:
Embodiment 1
Get 80 kilograms of lysine fermentation liquors, 5 kilograms of bacillus are (with the product after the medium culture, below the bacterium of each embodiment all identical), 3 kilograms of photosynthetic bacterias are (with the product after the medium culture, below each embodiment all identical), 15 kilograms of dregs of beans mix, obtain the livestock and poultry nutrients additive for biological feed, this product commodity are called " L-lysine raising poultry nutritive liquid ".
Embodiment 2
Get 100 kilograms of lysine fermentation liquors, 5 kilograms of bacillus, 3 kilograms of photosynthetic bacterias, 10 kilograms of peanut press pulps, add 2 kilograms in saccharomycete again, 1 kilogram of lactic acid bacteria, 12 kilograms of corn flour, mix, obtain the livestock and poultry nutrients additive for biological feed, this product commodity are called " L-lysine raising poultry nutritive liquid ".
Embodiment 3
Get 50 kilograms of fermenting lysine concentrates, 2 kilograms of bacillus, 4 kilograms of photosynthetic bacterias, 10 kilograms of dregs of beans add 2 kilograms in saccharomycete again, 1 kilogram of lactic acid bacteria, 10 kilograms of ferrous gluconates 0.5, copper sulphate 0.2, zinc sulfate 0.5 tapioca starch mix, and obtain the livestock and poultry nutrients additive for biological feed.
Embodiment 4
Get 30 kilograms of fermenting lysine solid products, 10 kilograms of bacillus, 8 kilograms of photosynthetic bacterias, 10 kilograms of rape cakes add 5 kilograms in saccharomycete again, and 10 kilograms of sweet potato powder mix, and obtain the livestock and poultry nutrients additive for biological feed.
Embodiment 5
Get 30 kilograms of fermenting lysine solid products, 10 kilograms of bacillus, 8 kilograms of photosynthetic bacterias, 10 kilograms of rape cakes, add 5 kilograms in saccharomycete again, 0.8 kilogram of ferrous gluconate, 0.3 kilogram in copper sulphate, 0.9 kilogram in zinc sulfate, 10 kilograms of rice meals, mix, obtain the livestock and poultry nutrients additive for biological feed.
Embodiment 6
Get 90 kilograms of lysine fermentation liquors, 5 kilograms of bacillus, 3 kilograms of photosynthetic bacterias, 10 kilograms of peanut press pulps, add 2 kilograms in saccharomycete again, 1 kilogram of lactic acid bacteria, 0.8 kilogram of ferrous gluconate, 0.3 kilogram in copper sulphate, 0.9 kilogram in zinc sulfate, 2 kilograms of grape calcium gluconates,, 0.3 kilogram of manganese sulfate, 0.2 kilogram of sodium selenate, 5 kilograms of rice meals, 10 kilograms in wheat bran powder, mix, obtain the livestock and poultry nutrients additive for biological feed.
Microorganism in the livestock and poultry nutrients additive for biological feed is cultivated embodiment:
01 batch
A82 lysine bacterial classification: slant activation 24 hours, 32 ℃ of cultivation temperature
Shaking bottle plants: loading amount 150ml/1000ml, 32 ℃ of cultivation temperature, incubation time 6～8 hours
0.2m
3: inoculum concentration 2%～10%, 32 ℃ of temperature, stirring ventilates cultivates air quantity 1: 0.3～1: 0.5, pressure 0.1Mpa, PH6.0～7.0, incubation time 5～10 hours.
5m
3: inoculum concentration 2%～10%, 32 ℃ of temperature, stirring ventilates cultivates air quantity 1: 0.3～1: 0.5, pressure 0.1Mpa, PH6.0～7.0, incubation time 5～10 hours.
50m
3: inoculum concentration 8%～15%, 32～34 ℃ of temperature stir to ventilate are cultivated, air quantity 1: 0.3～1: 1, pressure 0.1Mpa, PH6.0～7.5 adopt midway that stream adds technology, fermentation period 60～80 hours.
1
#Bacillus slant activation 24 hours, 28 ℃ of cultivation temperature
Shaking bottle plants: loading amount 150ml/1000ml, 28 ℃ of cultivation temperature, incubation time 6～10 hours.
0.2m
3: inoculum concentration 2%～15%, 28 ℃ of temperature, stirring ventilates cultivates air quantity 1: 0.3, pressure 0.1Mpa, PH6.0～7.0, incubation time 5～14 hours.
5m
3: inoculum concentration 8%～15%, 28 ℃ of temperature, stirring ventilates cultivates air quantity 1: 0.3, pressure 0.1Mpa, PH6.0～7.0, incubation time 16～24 hours.
2
#Photosynthetic bacteria anaerobism inclined-plane: connect the 100ml clear vial, constant temperature, anaerobism light was cultivated 3～5 days, inserting 11 transparent vessel constant temperature anaerobism light cultivated 4～6 days, insert 101 transparent vessel constant temperature, anaerobism light was cultivated 4～5 days, inserted 501 transparent vessels and cultivated sunshine, 25～35 ℃ of temperature, sunshine, abundance was 4～5 days.Inadequate 7～10 days of sunshine.
3
#Saccharomycete slant activation 24 hours, 28 ℃ of cultivation temperature.
Shaking bottle plants: loading amount 500ml/1000ml, static culture, 28 ℃ of temperature, incubation time 10～16 hours.
0.04m
3: inoculum concentration 2%～10%, 25～30 ℃ of temperature, incubation time 12～20 hours are cultivated in the gap ventilation.
0.2m
3: inoculum concentration 8%～15%, 25～30 ℃ of temperature, incubation time 16～24 hours are cultivated in the gap ventilation.
4
#The lactic acid bacteria inclined-plane inserts fresh milk activation 4～8 hours, 36 ℃ of temperature.
The straight cup of 500ml is planted: inoculum concentration 4%～8%, static culture, 36 ℃ of temperature, incubation time 3～4 hours.
0.04m
3: inoculum concentration 2%～8%, static culture, gap ventilation, 34～36 ℃ of temperature, incubation time 3～4 hours.
0.2m
3: inoculum concentration 4%～10%, static culture, gap ventilation, 34～36 ℃ of temperature, incubation time 3～6 hours.
Peanut press pulp: sheet peanut press pulp and cold water soaked 121 ℃ of steam sterilizings, pressure 0.1～0.15Mpa, 1 hour time 2～4 hours in 1: 3～1: 8 ratio.
Get the ripe mash 7m of above-mentioned fermenting lysine for 01 batch
3, bacillus 0.5m
3, photosynthetic bacteria 0.5m
3, saccharomycete 0.5m
3, lactic acid bacteria 0.5m
3, the peanut press pulp 1m after the sterilization
3, stir → bottle → sampling inspection, technical indicator, sanitary index is qualified → warehouse-in.
Application Example:
The biological raising poultry nutritive additive of L-lysine of the present invention pig-keeping experiment example.
One, pilot project
Numbering title (composition)
1 lysine fermentation liquor 8%
2 lysine fermentation liquor dilutions 0.5%
3 crystallization lysine dilutions 8%
4 crystallization lysine dilutions 0.5%
5 lysine fermentation liquor 8%+ photosynthetic bacteria+bacillus+saccharomycete+lactic acid bacteria+peanut press pulps
6 lysine fermentation liquor dilution 0.5%+ photosynthetic bacteria+bacillus+saccharomycete+lactic acid bacteria+peanut press pulps
7 crystallization lysine dilution 8%+ photosynthetic bacteria+bacillus+saccharomycete+lactic acid bacteria+peanut press pulps
8 crystallization lysine dilution 0.5%+ photosynthetic bacteria+bacillus+saccharomycete+lactic acid bacteria+peanut press pulps
9 blank
Two, pig-keeping experiment
Selecting the pig of 30-35kg is test pig, and every kind of prescription uses for 6 pigs respectively, and every day and feed are with feeding twice, and each every hurdle uses 60 milliliters.
Three, test situation
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| Every hurdle pig's head number | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
| Every hurdle pig heavy (kg) before the test | 208.6 | 198.4 | 205.3 | 203.1 | 190.4 | 201.5 | 204.7 | 196.5 | 197.1 |
| Test 60 days every hurdle pigs heavy (kg) | 446.6 | 378.8 | 389.6 | 352.4 | 499.6 | 468.2 | 400.1 | 359.4 | 324.5 |
| Every hurdle pig weightening finish (kg) | 238 | 180.4 | 184.3 | 149.3 | 309.2 | 266.8 | 195.4 | 162.9 | 127.4 |
| Average every daily gain in pigs (kg) | 0.66 | 0.5 | 0.51 | 0.41 | 0.86 | 0.74 | 0.54 | 0.45 | 0.35 |
| Every daily gain in pigs is than blank improve (%) | 88.6 | 42.8 | 45.7 | 17.1 | 145.7 | 111.4 | 54.3 | 28.6 |
Find out from above-mentioned result of the test, the effect that is numbered 5, No. 6 is the most obvious, illustrate that lysine fermentation liquor+photosynthetic bacteria of the present invention+bacillus and lysine fermentation liquor dilution 0.5%+ photosynthetic bacteria+bacillus are obvious to the gaining effect of pig, and the cost in numbering 6 is minimum.The viable bacteria that this product uses is China and allows 12 kinds of microorganism fodders using, and is nontoxic, have no side effect feeding safety.
Claims (11)
1, a kind of livestock and poultry nutrients additive for biological feed, it is characterized in that it comprises lysine bacterial classification tunning and bacillus, photosynthetic bacteria and dregs of beans, its composition and mass content are: bacillus 1-15%, photosynthetic bacteria 1-15%, dregs of beans 5-20%, all the other are lysine bacterial classification tunning.
2, livestock and poultry nutrients additive for biological feed according to claim 1, it is characterized in that the fermenting lysine product is: all products of lysine production overall process comprise intermediate products, finished product and their spraying, vacuum, evaporation and concentration product, dry product, the dilution product, absorbent articles, comprise the production waste liquid of intermediate products, finished product and their spraying, vacuum, evaporation and concentration product, dry product, absorbent articles with all waste liquids of lysine production overall process.
3, livestock and poultry nutrients additive for biological feed according to claim 1 is characterized in that bacillus comprises solid-state, semisolid, liquidly cultivates the concentrating of microbial inoculum and they, dilution, absorption, vacuum drying agent.
4, livestock and poultry nutrients additive for biological feed according to claim 1 is characterized in that photosynthetic bacteria is photosynthetic bacteria bacterium liquid and its concentrate, dilution, adsorbent, vacuum drying agent.
5, livestock and poultry nutrients additive for biological feed according to claim 1 is characterized in that dregs of beans comprises that peanut press pulp, soybean, flaxseed meal, rapeseed dregs are processed into the legume of powdery.
6, livestock and poultry nutrients additive for biological feed according to claim 1, it is characterized in that it also comprises other microorganism saccharomycete and lactic acid bacteria, plant amylum and trace element, its addition saccharomycete, lactic acid bacteria respectively account for the 1-5% of total additive quality, trace element accounts for 0.5-1%, and plant amylum accounts for 5-15%;
More than said saccharomycete comprise solid-state, semisolid, liquidly cultivate the concentrating of microbial inoculum and they, dilution, absorption, spray-drying, vacuum drying agent;
More than said lactic acid bacteria comprise the concentrating of semisolid, the liquid microbial inoculum of cultivating and they, dilution, absorption, vacuum drying agent;
More than said trace element comprise inorganic compound, the organic compound of iron, copper, zinc, calcium, selenium, manganese;
More than said plant amylum comprise cereal starch and potato starch.
7, a kind of animal and fowl fodder nourishing additive agent as claimed in claim 6, it is characterized in that saccharomycete is the raw material that saccharomycete is put into the fermentation beer of not hopping, be diluted in the culture medium of 8～12 ° of BX condition of culture: 25～30 ℃ of temperature, the product that obtains after the cultivation.
8, a kind of animal and fowl fodder nourishing additive agent as claimed in claim 6 is characterized in that lactic acid bacteria is that lactic acid bacteria is put into yeast extract or dusty yeast 0.3%～1.0%, soy peptone 0.1%～1.0%, glucose or lactose 0.3%～2.0%, K
2HPO
40.05%～0.5%, Tomato juice 5%～10%, in the culture medium of Tween 80 0.01%～0.1%, lactic acid bacteria condition of culture: 32～38 ℃ of temperature, the product that obtains after the cultivation.
9, the production method of livestock and poultry nutrients additive for biological feed according to claim 1, it is characterized in that production process is to get the molasses in sugar refinery, diluted concentration is 10-30BX, insert the lysine bacterial classification, temperature: 30～34 ℃, pressure: 0.05～0.12Mpa, air quantity: 1: 0.3～1: 1, PH6.0～7.5, rotating speed 250～550r.p.m, obtain lysine after the fermentation and enlarge bacterium, again by bacillus 1-15%, photosynthetic bacteria 1-15%, dregs of beans 5-20%, all the other are the ratio of lysine bacterial classification tunning, mix, and obtain the livestock and poultry nutrients additive for biological feed.
10, the production method of livestock and poultry nutrients additive for biological feed as claimed in claim 1 is characterized in that production process is earlier bacillus to be put into sucrose or molasses or glucose or starch 0.1%～2.0%, KH
2PO
40.05%～1.5%, MgSO
40.02%～0.2%, FeCl
30.0005%～0.005%, yeast extract or dusty yeast 0.01%～0.5%, (NH
4)
2SO
4In 0.01%～1.0% the culture medium, condition of culture: temperature: 28～32 ℃, pressure: 0.03～0.12Mpa, air quantity: 1: 0.1～1: 1, PH5.5～7.5, rotating speed 250～550r.p.m obtains bacillus and enlarges bacterium after the cultivation, again by bacillus 1-15%, photosynthetic bacteria 1-15%, dregs of beans 5-20%, all the other are the ratio of lysine bacterial classification tunning, mix, obtain the livestock and poultry nutrients additive for biological feed.
11, the production method of livestock and poultry nutrients additive for biological feed as claimed in claim 1 is characterized in that production process is earlier photosynthetic bacteria to be put into NH
4Cl 0.05%～0.2%, K
2HPO
40.01%～0.5%, MgCl
20.01%～0.2%, NaCl 0.05%～1.0%, yeast extract or dusty yeast 0.005%～0.5%, NaHCO
30.01%～2.0%, ethanol 0.1%～0.5% is used H
3PO
3Transfer in the culture medium of PH7.0 condition of culture: 25～32 ℃ of illumination of anaerobism obtain photosynthetic bacteria and enlarge bacterium after the cultivation, press bacillus 1-15%, photosynthetic bacteria 1-15%, dregs of beans 5-20% again, all the other are the ratio of lysine bacterial classification tunning, mix, and obtain the livestock and poultry nutrients additive for biological feed.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021156115A CN1171539C (en) | 2002-03-18 | 2002-03-18 | Nutrients additive for biological feed of livestock and fowls and its preparing process |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB021156115A CN1171539C (en) | 2002-03-18 | 2002-03-18 | Nutrients additive for biological feed of livestock and fowls and its preparing process |
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| Publication Number | Publication Date |
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| CN1372816A CN1372816A (en) | 2002-10-09 |
| CN1171539C true CN1171539C (en) | 2004-10-20 |
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| CNB021156115A Expired - Fee Related CN1171539C (en) | 2002-03-18 | 2002-03-18 | Nutrients additive for biological feed of livestock and fowls and its preparing process |
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013855A (en) * | 2012-11-06 | 2013-04-03 | 防城港市金沙海洋科技有限责任公司 | Compound microbial preparation and production process thereof |
| CN103766604B (en) * | 2014-01-16 | 2015-09-30 | 北京百丰天下生物科技有限公司 | A kind of preparation of composite probiotics preparations |
| CN104904997A (en) * | 2014-03-14 | 2015-09-16 | 卢国政 | A probiotic acid and a preparation method thereof |
| CN105211637A (en) * | 2014-06-25 | 2016-01-06 | 都江堰惠农生物技术有限责任公司 | Livestock biological feed nourishing additive agent and production method thereof |
| CN104591429A (en) * | 2014-12-25 | 2015-05-06 | 陈程 | Method for recycling tin ions in tin-containing industrial sewage |
| CN104528998A (en) * | 2014-12-25 | 2015-04-22 | 陈程 | Resource utilization method for copper ions in copper industrial sewage |
| CN105230966A (en) * | 2015-09-30 | 2016-01-13 | 河南科技大学 | Fermented feed for animals, and preparation method thereof |
| CN105379967B (en) * | 2015-10-21 | 2020-11-03 | 广东肇庆星湖生物科技股份有限公司 | Composite amino acid nutrition bag and preparation method thereof |
| CN110326805A (en) * | 2019-08-15 | 2019-10-15 | 青岛英赛特生物科技有限公司 | A kind of feed fermentation device and feed production method |
| CN113215051A (en) * | 2021-05-26 | 2021-08-06 | 南宁市拜欧生物工程有限责任公司 | Method for preparing feed probiotics by using lactobacillus through rice flour wastewater and passion fruit peel |
| CN113678951A (en) * | 2021-09-01 | 2021-11-23 | 齐齐哈尔龙江阜丰生物科技有限公司 | Preparation process of novel piglet food calling feed additive |
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| CN1372816A (en) | 2002-10-09 |
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