CN116711809A - Application of Bacillus subtilis in promoting animal production - Google Patents

Abstract

The invention relates to the technical field of microorganisms and discloses the application of Bacillus subtilis in promoting animal production. The research team of the present invention screened out a kind of Bacillus subtilis. After research, it was found that this Bacillus subtilis can comprehensively improve the expression of tight junction proteins in broilers through a multi-target mechanism. It can also promote the immune function of broilers and improve the intestinal health of broilers. And microecological balance, multiple aspects work together to promote broiler production. The screened Bacillus subtilis is used to promote the production of broiler chickens, thereby providing a method for promoting the production of broiler chickens. Using the method provided by the present invention can reduce the feed-to-weight ratio and mortality rate of the broiler chickens raised, and greatly improve the performance of the broiler chickens. productive forces.

Description

Application of Bacillus subtilis in promoting animal production

Technical field

The present invention relates to the field of microbial technology, and in particular to the application of Bacillus subtilis in promoting animal production.

Background technique

Conventional animal husbandry has traditionally used subtherapeutic doses of antibiotics to improve performance and prevent disease. The mechanism by which antibiotics play a beneficial role as feed additives can be divided into the following aspects: ① inhibit and kill pathogenic bacteria, improve animal immune function, and achieve the effects of disease prevention and treatment; ② regulate the intestinal microflora and reduce its maintenance needs , Reduce the loss of nutrients; ③ Improve intestinal permeability, thereby increasing the absorption of nutrients in the intestine; ④ Slow down intestinal peristalsis, thereby increasing the time that chyme stays in the intestine, and improve the digestion and absorption rate. Antibiotics as feed additives can improve animal performance and reduce morbidity and mortality caused by clinical and subclinical diseases.

Although antibiotics are beneficial to animal health and productivity, antibiotic resistance and residues in animal products are threatening human health. For this reason, starting from July 1, 2021, my country's Ministry of Agriculture and Rural Affairs Announcement No. 194 was officially promulgated, prohibiting feed production companies from producing commercial feed containing growth-promoting drug feed additives. However, antibiotic restrictions have led to problems such as reduced animal performance and feed conversion, as well as increased incidence of certain animal diseases. Therefore, developing safe, non-toxic and side-effect antibiotic alternatives to improve animal productivity is a critical and urgent task.

Probiotics refer to live microorganisms that can have beneficial effects on the health of the host when ingested in certain amounts. It mainly inhibits the growth of intestinal pathogenic bacteria through various mechanisms such as improving intestinal microecology, enhancing intestinal barrier function, balancing immune response, and synthesizing antibacterial substances. Commonly used probiotics include Bacillus, Lactobacillus, Bifidobacterium, etc. Probiotic products are used as feed additives in poultry farming. Probiotic strains have the following functions: ① Improve the diversity and stability of intestinal microbial flora; ② Balance pro-inflammatory factors and anti-inflammatory factors (regulation of immune response); ③ Compete for bacterial flora adhesion sites; ④ Secrete antibacterial substances, thereby Improve poultry performance. In addition, previous studies have shown that probiotics can play beneficial effects on the body’s antioxidant capacity, digestive enzymes, nutrient absorption and reduction of apoptosis, ultimately benefiting the intestinal health and performance of broiler chickens.

Among the many probiotic strains that can be used as additives, Bacillus subtilis is currently one of the most widely used feed probiotics. Bacillus subtilis can improve feed conversion rate, enhance immunity, and improve intestinal flora balance. It is an ideal microbial feed additive that can replace growth-promoting antibiotics.

Contents of the invention

In order to solve the above technical problem of improving animal productivity, the present invention provides the application of Bacillus subtilis in promoting animal production. The research team of the present invention screened out a Bacillus subtilis. After research, it was found that this Bacillus subtilis can comprehensively improve the expression of tight junction proteins in broilers through a multi-target mechanism. It can also promote the immune function of broilers and improve the intestinal health of broilers. And microecological balance, multiple aspects work together to promote broiler production.

The specific technical solutions of the present invention are:

On the one hand, the present invention provides an application of Bacillus subtilis in promoting animal production. The Bacillus subtilis is deposited in the China Typical Culture Collection Center, the deposit address is Wuhan, China, and the deposit date is June 1, 2023. The deposit number is CCTCC M 2023897.

The research team of the present invention isolated and screened a strain of Bacillus subtilis from the soil of a chicken farm. After sequencing, it was identified as a new strain and named Bacillus subtilis ZJU16. The research team of the present invention deposited the screened Bacillus subtilis ZJU16 in the China Type Culture Collection Center (CCTCC) on June 1, 2023. The deposit number is CCTCC M 2023897. The strain is classified and named Bacillus subtilis. subtilis ZJU16 (BS ZJU16), the address is No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province.

The 16s rDNA sequence of the above-mentioned Bacillus subtilis ZJU16 is shown in SEQ ID No. 1.

Research has found that the above-mentioned Bacillus subtilis ZJU16 can be used in the preparation of functional feed additives that promote animal production.

Specifically, the present invention takes broiler chickens as the object, and conducts research on Bacillus subtilis ZJU16 to improve animal health and promote animal production. Specifically:

① The research team of the present invention fed broilers with Bacillus subtilis ZJU16 and found that from the perspective of the entire growth period of broiler chickens, feeding Bacillus subtilis ZJU16 compared with the control group that did not feed Bacillus subtilis ZJU16, The feed-to-weight ratio and mortality rate are reduced, and the effect of reducing the feed-to-weight ratio and mortality rate is equivalent to that of feeding antibiotics; ② At the same time, the research team of the present invention found that compared with the control group that did not feed Bacillus subtilis ZJU16, The GSH-Px and SOD activities in the jejunal mucosa of broiler chickens fed Bacillus subtilis ZJU16 increased by 65.10% (P<0.01) and 23.39 (P<0.05) respectively, and the MDA level showed a decreasing trend, indicating that Bacillus subtilis ZJU16 improves the body’s antioxidant capacity;

③ The research team of the present invention also analyzed the villus morphology and structure of epithelial cells in duodenum, jejunum and ileum of broiler chickens fed Bacillus subtilis ZJU16 compared with those without feeding, and found that those fed Bacillus subtilis ZJU16 The morphological structure of the epithelial villi in the small intestine of broiler chickens is clear and the villi are long, neat and compact, confirming that feeding Bacillus subtilis ZJU16 can effectively improve the morphological structure of the small intestinal villi; at the same time, the research team of the present invention confirmed that feeding Bacillus subtilis ZJU16 subtilis ZJU16 can improve the physical barrier of intestinal epithelium to protect epithelial cells, increase the activity of digestive enzymes in the intestinal mucosa, and regulate the composition of intestinal flora in broilers.

It can be seen from this that the Bacillus subtilis provided by the present invention can ensure the health of broiler chickens and promote the production of broiler chickens through multi-faceted interactions.

On the other hand, the present invention also provides a functional feed for promoting animal production, the feed contains Bacillus subtilis, and the Bacillus subtilis is deposited in the China Typical Culture Collection Center, and the deposit address is Wuhan, China. The deposit date is June 1, 2023, and the deposit number is CCTCC M 2023897.

As a preferred embodiment of the above technical solution of the present invention, Bacillus subtilis is added in the form of bacterial powder.

Specifically, the present invention provides a preparation method of the bacterial powder including the following steps:

(1) Take the Bacillus subtilis and conduct fermentation culture to obtain fermentation liquid;

(2) Concentrate the fermentation broth obtained in step (1), freeze-dry, and grind to obtain concentrated bacterial powder.

The invention provides a feed to which Bacillus subtilis ZJU16 is added in the form of bacterial powder. That is, Bacillus subtilis ZJU16 is first fermented and cultured to obtain a fermentation liquid, which is concentrated, freeze-dried, and ground to prepare powdered bacterial cells.

Further, the preparation method of the bacterial powder also includes the following steps: adding starch to the concentrated bacterial powder for dilution.

When high-concentration concentrated bacterial powder is added directly to feed, Bacillus subtilis ZJU16 will be unevenly distributed. It can be diluted by adding starch to the high-concentration concentrated bacterial powder before adding it.

At the same time, the present invention provides a method for promoting broiler production, based on the above-mentioned functional feed for promoting animal production, including the following steps:

The broiler chickens are fed with the functional feed in the litter flat raising mode;

Wherein, in the functional feed, the effective content of Bacillus subtilis is 0.5-9.0×10 ¹² CFU/ton.

To promote the production of broiler chickens, the content of Bacillus subtilis ZJU16 in the feed fed to broiler chickens when raised in the litter-flat mode has a direct impact on the results of promoting the production of broiler chickens. The amount of Bacillus subtilis ZJU16 should be within the range of 0.5 to 9.0×10 ¹² CFU/ton. If the amount is too low or too high, it will not be able to promote broiler production.

As a preferred embodiment of the above technical solution of the present invention, the feeding starts from the 22nd day of age of the broiler chickens until the end of the feeding, or the feeding starts from the 1st day of age until the end of the feeding.

Aiming at the production of broiler chickens, the present invention provides a method for promoting the production of broiler chickens, that is, using the litter flat raising mode and feeding the broiler chickens with the above-mentioned functional feed instead of the ordinary basic diet. The productivity of the broiler chickens will be greatly improved.

Feeding can start from the 22nd day of broiler age until the end of feeding. The present invention found in the research that in the early stage of raising broiler chickens, that is, from 1 to 21 days old, the daily weight gain, average daily feed intake, feed-to-weight ratio and mortality rate of broiler chickens fed Bacillus subtilis ZJU16 are better than those without feeding. Compared with the broilers fed Bacillus subtilis ZJU16, there was no significant difference. However, in the middle and late stages of feeding, the feed-to-weight ratio and mortality rate of the broilers fed Bacillus subtilis ZJU16 were lower than those without feeding. Therefore, the feeding Feeding Bacillus subtilis ZJU16, that is, using the functional feed instead of the ordinary basic diet to feed broiler chickens, can start from the 22nd day of the broiler age until the end of feeding to save costs.

The feeding can also start from any day when the age is less than 22 days until the end of feeding.

Among them, the litter flat raising mode described in the present invention refers to laying a certain thickness of litter in the chicken house, allowing the chickens to move freely, forage and rest on the litter to achieve the purpose of growth and breeding. The litter flat breeding model is a relatively scientific and humane broiler chicken breeding model, which can improve the breeding efficiency and production quality of broiler chickens. In the present invention, in the basal diet of litter, broilers are fed with functional feed instead of the ordinary basal diet.

Compared with the existing technology, the present invention has the following technical effects:

The Bacillus subtilis screened by the research team of the present invention can comprehensively improve the expression of tight junction proteins in broilers through a multi-target mechanism of action. It can also promote the immune function of broilers, improve the intestinal health and microecological balance of broilers, and work together in many aspects to promote Broiler production.

The screened Bacillus subtilis is used to promote the production of broiler chickens. The present invention also provides a method for promoting the production of broiler chickens. Using the method provided by the present invention can reduce the feed-to-weight ratio and mortality rate of the broiler chickens raised, and greatly improve Broiler productivity.

Description of the drawings

Figure 1 is a microstructure diagram of the epithelial villi of the duodenum, jejunum, and ileum of broiler chickens in different treatment groups in Example 2;

Figure 2 is a diagram showing the results of tight junction protein expression of broiler chickens in different treatment groups in Example 2;

Figure 3 is a graph showing the duodenal mucosal digestive enzyme activity results of broiler chickens in different treatment groups in Example 2;

Figure 4 is a graph showing the results of the antioxidant function of the jejunal mucosa of broiler chickens in different treatment groups in Example 2;

Figure 5 is a graph showing the relative abundance results of cecal flora of broiler chickens in different treatment groups in Example 2;

Figure 6 is a diagram showing the serum cytokine measurement results of broiler chickens in different treatment groups in Example 2.

Detailed ways

The present invention will be further described below in conjunction with examples. The Bacillus subtilis used in the following examples is Bacillus subtilis ZJU16 selected by the research team of the present invention.

Example 1 Identification of Bacillus subtilis ZJU16

The research team of the present invention isolated and screened a Bacillus subtilis from XXXX, and identified the strain:

Extract the genomic DNA of the strain, use the genome as a template, and use universal primers 27F: AGAGTTTGATCCTGGCTCAG and 1492R: GGTTACCTTGTTACGACTT to amplify its 16S rDNA fragment. The amplified product is detected by agarose gel electrophoresis and sent to a sequencing company for sequencing. The sequencing data was uploaded to the NCBI database for BLAST comparison analysis. The comparison results showed that the similarity with Bacillus subtilis was 99%. Therefore, the strain was determined to be Bacillus subtilis. Moreover, because the sequencing results did not completely match those in the database, the strain was determined to be a newly identified strain and named Bacillus subtilis ZJU16. Wherein, the sequence of the 16SrDNA fragment is shown in SEQ ID No. 1.

The research team of the present invention isolated and screened Bacillus subtilis ZJU16 from the soil of the above-mentioned chicken farm, and deposited it in the China Type Culture Collection Center (CCTCC) on June 1, 2023. The preservation number is CCTCC M 2023897. This strain The classification name is Bacillus subtilis ZJU16 (BSZJU16), and the address is No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province.

Example 2 Promotional effect of Bacillus subtilis ZJU16 on the growth of broiler chickens (1) Experimental design and feeding management

The experiment selected 720 one-day-old healthy broiler chicks of the Lingnan yellow variety (fed with mixed male and female) and randomly divided them into 3 treatment groups, with 6 replicates in each group and 40 chickens in each replicate. The 3 treatment groups are:

①Control group: fed basic diet;

② Bacillus subtilis group: The Bacillus subtilis preparation was added to the basic diet at an amount of 500g/t feed; among them, the effective content of Bacillus subtilis ZJU16 in the Bacillus subtilis preparation was 2.0×10 ⁹ CFU/g;

③ Antibiotic group: The antibiotic enramycin is added to the basal diet level, and the addition amount is 10g/t feed (the premix enramycin content is 4%, and the enramycin addition amount is 10g/t feed , then the amount of premix added to the feed is 250g/t feed).

The experiment adopted the litter flat raising mode, free access to food and water, routine feeding and management, and the number of dead chickens and their weight were recorded daily. The experimental period lasted 63 days and was divided into two stages: the early stage of feeding (1 to 21 days) and the later stage (22 to 63 days).

(2) The effects of feeding Bacillus subtilis ZJU16 on the production performance of broiler chickens were recorded in each group from 1 to 21 days of age (early feeding period), 22 to 63 days of age (late feeding period), and 1 to 63 days of age (full feeding period) The average daily feed intake (ADFI), daily gain (ADG), and feed to weight ratio (F/G) are shown in Table 1.

Table 1

It can be seen from Table 1 that in the early growth period of broiler chickens (1 to 21 days of age), there was no significant difference in daily weight gain, average daily feed intake, feed-to-weight ratio and mortality rate of broiler chickens in the Bacillus subtilis group compared with the control group ( P>0.05), and compared with the antibiotic group, the daily feed intake was increased (P<0.05); from the perspective of the later growth period and the whole period of broiler chicken growth, the Bacillus subtilis group reduced the feed-to-weight ratio and mortality compared with the control group (P <0.05), and compared with the antibiotic group, there was no significant difference in each index (P>0.05).

It can be seen that adding Bacillus subtilis ZJU16 to the diet has a great effect on improving the production performance of broiler chickens. It can replace the effect of growth-promoting antibiotics, and further, it can increase the daily feed intake of broiler chickens in the early growth period. .

(3) Effect of feeding Bacillus subtilis ZJU16 on the intestinal function of broiler chickens. After the 63-day experimental period, 2 test roosters with similar body weight were selected from each group for each repetition, totaling 36 chickens. They were slaughtered and the intestinal tissues and samples were collected. Contents and serum samples, the microstructure of duodenum, jejunum, and ileum epithelial villi of random broiler chickens in the three treatment groups are shown in Figure 1. Figure 2 shows the expression of tight junction proteins in the random broiler chickens in the three treatment groups. Figure 3 The activity of digestive enzymes in the duodenal mucosa of random broiler chickens in the three treatment groups. Figure 4 shows the antioxidant function of the jejunal mucosa of the random broiler chickens in the three treatment groups. In Figures 1 to 4, Ctr is the control group, BS is the Bacillus subtilis group, and ER is the antibiotic group. In Figure 1, the pictures in the left column show the structure of duodenal epithelial villi, the pictures in the middle column show the structure of jejunal epithelial villi, and the pictures in the right column show the structure of ileal epithelial villi.

As can be seen from Figure 1, compared with the morphological structure of the villi of the epithelial cells of the small intestine (duodenum, jejunum and ileum) of the control group, the morphological structure of the epithelial villi of the small intestine of the Bacillus subtilis group is clear and the villi are longer, neat and compact; compared with the small intestine of the antibiotic group The morphological structure of epithelial villi is basically the same. It can be seen that adding Bacillus subtilis ZJU16 to the diet can effectively improve the morphological structure of small intestinal villi.

As can be seen from Figure 2, compared with the control group, the Bacillus subtilis group up-regulated the expression of intestinal epithelial tight junction ZO-1 protein; it was basically consistent with the expression of intestinal epithelial tight junction ZO-1 protein in the antibiotic group. It can be seen from this that, Adding Bacillus subtilis ZJU16 to the diet can improve the physical barrier of intestinal epithelium to protect epithelial cells.

As can be seen from Figure 3, compared with the control group, the amylase and trypsin activities of the duodenal mucosa in the Bacillus subtilis group increased by 5.73% (P>0.05) and 10.08% (P>0.05) respectively, which was ten times higher than that in the antibiotic group. The amylase and trypsin activities of the duodenal mucosa are basically the same. It can be seen that adding Bacillus subtilis ZJU16 to the diet can increase the activity of digestive enzymes in the intestinal mucosa.

As can be seen from Figure 4, compared with the control group, the GSH-Px and SOD activities in the jejunal mucosa of the Bacillus subtilis group increased by 65.10% (P<0.01) and 23.39 (P<0.05) respectively, and the MDA level showed a decreasing trend. It can be seen that adding Bacillus subtilis ZJU16 to the diet can improve the body's antioxidant capacity.

(4) Effects of feeding Bacillus subtilis ZJU16 on the immune function of broiler chickens. After the 63-day experimental period, 2 test roosters with similar weights were selected from each group for each repetition, totaling 36 roosters. They were slaughtered and the intestinal tissues and contents were collected. Materials and serum samples were taken to measure the relative abundance of cecal flora in the three treatment groups. The results are shown in Figure 5.

As can be seen from Figure 5, at the phylum level, the main dominant bacteria in the cecal flora of the three groups are Firmicutes; compared with the control group, there is no significant difference in the abundance of Firmicutes in the cecum of the Bacillus subtilis group. (P>0.05), and compared with the antibiotic group, the abundance of Firmicutes was significantly increased (P<0.05). At the genus level, the dominant bacteria of the three groups of cecal flora are Bacteroides, Campylobacter, Helicobacter and Faecalibacterium. Compared with the control group, the Bacillus subtilis group reduced the relative abundance of Bilophila; while the antibiotic group increased the relative abundance of Bacteroides (P<0.05) and reduced the relative abundance of Ruminococcaceae and Odoribacter (P<0.05). Compared with the antibiotic group, the Bacillus subtilis group increased the relative abundance of Alistipes, Ruminococcaceae, Parabacteroide, Blautia and Sutterella (P<0.05). It can be seen that adding Bacillus subtilis ZJU16 to the diet can regulate the intestinal flora composition of broiler chickens.

(5) Effect of feeding Bacillus subtilis ZJU16 on the immune function of broiler chickens during the experimental period. After the 63-day test, 2 test roosters of similar weight were selected from each group for each repetition, a total of 36 roosters were slaughtered, and serum samples were collected. The serum cytokines of broiler chickens in the treatment group were measured, and the results are shown in Figure 6.

As can be seen from Figure 6, compared with the control, the serum levels of pro-inflammatory factors IL-1β and IL-6 in the Bacillus subtilis group increased by 31.25% (P<0.01) and 25.37% (P<0.01) respectively, while the level of IL-10 It was reduced by 5.90% (P<0.05), and there was no significant difference in the level of TNF-α (P>0.05). It can be seen that adding Bacillus subtilis ZJU16 to the diet can improve the immune function of broiler chickens.

Example 3 Optimization test of feeding conditions for Bacillus subtilis ZJU16 broiler chickens: 720 one-day-old Lingnan yellow breed healthy broiler chicks (male and female mixed feeding) were randomly divided into 5 treatment groups, with 6 repetitions in each group. 20 chickens per replicate. The 5 treatment groups are:

① Low addition amount group: Bacillus subtilis preparation is added to the basic diet every day, and the addition amount is 100g/t feed; among them, the effective content of Bacillus subtilis ZJU16 in the Bacillus subtilis preparation is 2.0×10 ⁹ CFU/ g;

②Appropriate amount group A: Bacillus subtilis preparation is added to the basic diet every day, and the addition amount is 250g/t feed; among them, the effective content of Bacillus subtilis ZJU16 in the Bacillus subtilis preparation is 2.0×10 ⁹ CFU/ g;

③Appropriate amount group B: Bacillus subtilis preparation is added to the basic diet every day, and the addition amount is 500g/t feed; among them, the effective content of Bacillus subtilis ZJU16 in the Bacillus subtilis preparation is 2.0×10 ⁹ CFU/ g;

④Appropriate amount group C: Bacillus subtilis preparation is added to the basic diet every day, and the addition amount is 4500g/t feed; among them, the effective content of Bacillus subtilis ZJU16 in the Bacillus subtilis preparation is 2.0×10 ⁹ CFU/ g;

⑤High addition amount group: Bacillus subtilis preparation is added to the basic diet every day, and the addition amount is 5000g/t feed; among them, the effective content of Bacillus subtilis ZJU16 in the Bacillus subtilis preparation is 2.0×10 ⁹ CFU/ g.

The experiment adopted the litter flat raising mode, free access to food and water, routine feeding and management, and the number of dead chickens and their weight were recorded daily. The experimental period lasted 63 days and was divided into two stages: the early stage of feeding (1 to 21 days) and the later stage (22 to 63 days). The average daily feed intake (ADFI), daily gain (ADG), and feed-to-weight ratio (F/G) from 1 to 63 days of age (full feeding period) are shown in Table 2.

Table 2

It can be seen from Table 2 that the feed-to-weight ratio and mortality rate of broilers in groups ② to ④ are lower, while the feed-to-weight ratio and mortality rate of broiler chickens in groups ① and ⑤ are higher. The results were analyzed, and from the comparison of the feeding conditions of each group, the addition amounts of Bacillus subtilis in groups ① to ⑤ were 2.0×10 ¹¹ CFU/ton, 0.5×10 ¹² CFU/ton, and 1.0×10 ¹² respectively. CFU/ton, 9.0×10 ¹² CFU/ton, 10.0×10 ¹² CFU/ton, the amount of Bacillus subtilis ZJU16 added to the feed of each group is different. It can be seen that the addition of Bacillus subtilis ZJU16 The amount should be within a certain range. If the amount is too low or too high, it will not be able to promote broiler production.

Among them, the effective content of the Bacillus subtilis preparation Bacillus subtilis ZJU16 used in the embodiment of the present invention is 2.0×10 ⁹ CFU/g, and its preparation method is: taking out the Bacillus subtilis ZJU16 strain stored in the ultra-low temperature refrigerator , transfer the pure Bacillus subtilis ZJU16 strain on a sterile ultra-clean operating table. After culturing for 12 hours, the strain is also inoculated into an intermediate fermenter containing sterilized culture medium under sterile conditions, and then Transfer to the fermentation tank of the final production scale; after 24 hours of fermentation, use gentle technology to concentrate the Bacillus subtilis ZJU16 cells 30 times, thereby reducing shear force and air mixing, and then the concentrated Bacillus subtilis ZJU16 subtilis ZJU16 cells are quickly frozen into small pellets. The final Bacillus subtilis ZJU16 cell concentrate is obtained through freeze-drying. The concentrate is placed in a stainless steel container on a multi-directional rotating arm. The freeze-dried concentrate is first ground and then Mix to achieve the uniformity of Bacillus subtilis ZJU16 bacterial powder; finally freeze-dry and concentrate the Bacillus subtilis ZJU16, and use starch as the dilution carrier to prepare a Bacillus subtilis preparation that can be added to feed. Among them, the effective content of Bacillus subtilis ZJU16 is 2.0×10 ⁹ CFU/g.

The ingredients and nutritional levels (% on a feeding basis) of the basal diet of the present invention are as follows:

Each kilogram of diet provides: Fe 80mg, Cu 8mg, Zn 60mg, Mn 80mg, I 0.35mg, Se 0.15mg, VA9600IU, VD31500IU, VE20mg, Vk31 mg, VB12.2 mg, 2VB2, VB64.2 mg, VB120.012 mg, nicotinamide 42 mg, D-calcium pantothenate 12 mg, folic acid 1.0 mg, D-biotin 0.18 mg, choline 800 mg.

The raw materials and equipment used in the present invention, unless otherwise specified, are all commonly used raw materials and equipment in this field; the methods used in the present invention, unless otherwise specified, are all conventional methods in this field.

The above are only preferred embodiments of the present invention and do not limit the present invention in any way. Any simple modifications, changes and equivalent transformations made to the above embodiments based on the technical essence of the present invention still belong to the technical solution of the present invention. scope of protection.

Claims (8)

1. The application of Bacillus subtilis in promoting animal production, characterized in that: the Bacillus subtilis is deposited in the China Typical Culture Collection Center, the deposit address is Wuhan, China, the deposit date is June 1, 2023, and the deposit number is CCTCC M 2023897. 2. The application according to claim 1, characterized in that: the 16s rDNA sequence of Bacillus subtilis is shown in SEQ ID No. 1. 3. A functional feed for promoting animal production, characterized in that: the feed contains Bacillus subtilis, and the Bacillus subtilis is deposited in the China Typical Culture Collection Center, the deposit address is Wuhan, China, and the deposit date is On June 1, 2023, the deposit number is CCTCC M 2023897. 4. The feed according to claim 3, wherein the Bacillus subtilis is added in the form of bacterial powder. 5. The feed according to claim 4, characterized in that: the preparation method of the bacteria powder includes the following steps: (1) Take the Bacillus subtilis and conduct fermentation culture to obtain fermentation liquid; (2) Concentrate the fermentation broth obtained in step (1), freeze-dry, and grind to obtain concentrated bacterial powder. 6. The feed according to claim 5, characterized in that: the preparation method of the bacteria powder further includes the following steps: Starch is added to the concentrated bacterial powder for dilution. 7. A method for promoting broiler production, based on a functional feed for promoting animal production according to claim 3, characterized in that: comprising the following steps: The broiler chickens are fed with the functional feed in the litter flat raising mode; Wherein, in the functional feed, the effective content of Bacillus subtilis is 0.5~9.0×1012 CFU/ton. 8. The method according to claim 7, characterized in that: the feeding starts from the 22nd day of age of the broiler chickens until the end of the feeding.