CN116098166A - South Hunan mechanically-inserted double-cropping rice cold-resistant agent and application method thereof - Google Patents

Abstract

The invention belongs to the technical field of cold-resistant agents, and provides a south Hunan mechanical-insert double-cropping rice cold-resistant agent and a use method thereof. The invention provides a south Hunan mechanically-inserted double-cropping rice cold-resistant agent which is composed of the following raw materials in parts by weight: 1 to 3 parts of zinc sulfate, 1 to 3 parts of manganese sulfate, 1 to 3 parts of ammonium chloride, 0.5 to 1.5 parts of beta-cyclodextrin and 0.5 to 1.5 parts of anti-falling agent. The cold-resistant agent and the use method thereof solve the problems that the 'reverse spring cold' of the production of early rice in Hunan province and the root and stem of rice in the seedling stage are not generated, the yield of late rice is reduced due to 'cold dew wind', and the like, and provide technical and theoretical support for further improving the yield of rice in the south Hunan province and realizing the stress resistance and stable yield of the rice.

Description

A cold-resistant agent for machine-transplanted double-cropping rice in southern Hunan and its application method

technical field

The invention relates to the technical field of anti-cold agents, in particular to an anti-cold agent for machine-transplanted double-cropping rice in southern Hunan and its application method.

Background technique

In southern Shonan, the early spring cold injury "late spring cold" occurs frequently, and late rice is often invaded by "cold dew wind", which has a great impact on the safe production of double-cropping rice, especially machine-transplanted double-cropping rice. At present, rice machine-transplanted rice is developing rapidly, but the machine-transplanted rice has a long period of greening, which greatly increases the risk of double-cropping late rice encountering "cold dew wind". Therefore, it is particularly necessary to study a cold-resistant agent suitable for machine-transplanted double-cropping rice.

Contents of the invention

In order to overcome the above-mentioned defects in the prior art, the invention provides a cold-resistant agent for machine-transplanted double-cropping rice in southern Hunan and its application method.

In order to achieve the above-mentioned purpose of the invention, the present invention provides the following technical solutions:

The invention provides a cold-resistant agent for machine-transplanted double-cropping rice in southern Hunan, which is composed of the following raw materials in parts by weight: 1 to 3 parts of zinc sulfate, 1 to 3 parts of manganese sulfate, 1 to 3 parts of ammonium chloride, β- 0.5-1.5 parts of cyclodextrin and 0.5-1.5 parts of anti-falling element.

The present invention also provides a method for using the anti-cold agent for machine-transplanted double cropping rice in southern Hunan, comprising the following steps:

(1) Mix the anti-cold agent for double-cropping rice in southern Hunan with water to obtain a mixed solution;

(2) Spray the above-mentioned mixed solution at the stage of one leaf and one heart of early rice or after turning green, and spray the above-mentioned mixed solution at the beginning of earing stage of late rice.

Preferably, the mixing ratio of the cold-resistant agent for machine-transplanted double-cropping rice in southern Hunan and water in step (1) is 45-55 g: 35000-45000 mL.

Preferably, the amount of spraying in step (2) is 35000-45000 mL/mu.

Compared with the prior art, the beneficial effects of the present invention are as follows:

The anti-cold agent provided by the invention and its use method solve the problems of "cold spring" faced by early rice production in Hunan, the failure of rice seedlings to grow at the seedling stage, and the production reduction of late rice due to "cold dew wind". Stress-resistant and stable yield of rice provides technical and theoretical support.

Description of drawings

Fig. 1 is the figure of influence of cold resistance agent of the present invention and spraying period on early rice stem and tiller dynamics.

Detailed ways

The invention provides a cold-resistant agent for machine-transplanted double-cropping rice in southern Hunan, which is composed of the following raw materials in parts by weight: 1 to 3 parts of zinc sulfate, 1 to 3 parts of manganese sulfate, 1 to 3 parts of ammonium chloride, β- 0.5-1.5 parts of cyclodextrin and 0.5-1.5 parts of anti-falling element. Wherein, the anti-falling agent is p-chlorophenoxyacetic acid.

In the present invention, the cold-resistant agent for machine-transplanted double-cropping rice in southern Hunan is preferably composed of raw materials comprising the following parts by weight: 1-3 parts of zinc sulfate, more preferably 1.5-2.5 parts, and even more preferably 2 parts 1-3 parts of manganese sulfate, more preferably 1.5-2.5 parts, more preferably 2 parts; 1-3 parts of ammonium chloride, more preferably 1.5-2.5 parts, more preferably 2 parts; Dextrin is 0.5-1.5 parts, more preferably 0.8-1.2 parts, still more preferably 1 part; anti-falling agent is 0.5-1.5 parts, more preferably 0.8-1.2 parts, still more preferably 1 part.

The present invention also provides a method for using the anti-cold agent for machine-transplanted double cropping rice in southern Hunan, comprising the following steps:

(1) Mix the anti-cold agent for double-cropping rice in southern Hunan with water to obtain a mixed solution;

(2) Spray the above-mentioned mixed solution at the stage of one leaf and one heart of early rice or after turning green, and spray the above-mentioned mixed solution at the beginning of earing stage of late rice.

In the present invention, the mixing ratio of the cold-resistant agent for machine-transplanted double cropping rice in southern Hunan and water in step (1) is preferably 45-55g:35000-45000mL, more preferably 50g:40000mL.

In the present invention, the amount of spraying in step (2) is preferably 35000-45000 mL/mu, more preferably 38000-42000 mL/mu, even more preferably 40000 mL/mu.

The technical solutions provided by the present invention will be described in detail below in conjunction with the examples, but they should not be interpreted as limiting the protection scope of the present invention.

Example 1

The invention provides a cold-resistant agent for machine-planted double-season rice in southern Hunan, which is composed of the following raw materials in parts by weight: 1 part of zinc sulfate, 1 part of manganese sulfate, 1 part of ammonium chloride, 0.5 parts of β-cyclodextrin and Drop 0.5 part.

Example 2

The invention provides a cold-resistant agent for machine-planted double-cropping rice in southern Hunan, which is composed of the following raw materials in parts by weight: 2 parts of zinc sulfate, 2 parts of manganese sulfate, 2 parts of ammonium chloride, 1 part of β-cyclodextrin and Drop 1 part.

Example 3

The invention provides a cold-resistant agent for machine-planted double-cropping rice in southern Hunan, which is composed of the following raw materials in parts by weight: 3 parts of zinc sulfate, 3 parts of manganese sulfate, 3 parts of ammonium chloride, 1.5 parts of β-cyclodextrin and Drop 1.5 parts.

Experimental example 1

Experimental materials: The early rice is Zhongjiazao 17, which is an indica-type conventional early rice, with a total growth period of 109 days; the late rice is Hyou 518, which is an indica-type three-line hybrid rice, with an average growth period of 112.9 days. The anti-cold agent selects the short buttress (uniconazole powder, active ingredient 20%) produced by Tuyouta Company, the S-induced absin (abscisic acid water agent, active ingredient 0.1%) produced by Zhifu Company and the implementation of the present invention. Cold-resistant agent for machine-transplanted double-cropping rice in Shonan in Example 2.

Experimental location: A double-cropping rice experiment was carried out in Xidu Town, Hengyang County, Hunan Province in 2018. The basic physical and chemical characteristics of the soil for the test field are: organic matter 25.20g/kg, total nitrogen 1.50g/kg, alkali-hydrolyzed nitrogen 162.30mg/kg, total phosphorus 0.64g/kg, available phosphorus 9.60mg/kg, total potassium 19.30g/kg kg, available potassium 102.43mg/kg, pH6.22. The size of the seedling tray for testing is 58cm×28cm.

Experimental design: The experiment set up two factors of anti-cold agent type and spraying period. The types of anti-cold agents are dwarf pier (C1), S-inducer (C2) and the anti-cold agent for machine-transplanted double-cropping rice in southern Hunan (C3) in Example 2 of the present invention. The application concentration is the recommended concentration in the product manual (C1 soaking concentration is 1000 times of liquid, spraying concentration is 30g per mu mixed with 30 kg of water; C2 soaking and spraying concentration are both 200 times liquid; C3 soaking and spraying concentration are both 50g 40 kg of water). During the early rice spraying period, there were five periods: soaking seeds (D1), spraying with one leaf and one center (D2), spraying with two leaves and one heart (D3), spraying before transplanting (D4), and spraying after turning green (D5). The spraying period was divided into four periods: booting stage (F1), initial heading stage (F2), full heading stage (F3), and one week after full heading stage (F4). In addition, in order to ensure that late rice encounters low temperature adversity, a delayed sowing experiment for late rice was set up. Early rice was soaked on March 24th (D1 treatment was soaked according to the concentration recommended by the above product manual, and the remaining treatments were soaked in clear water), sowed on March 28th (105g/tray of dry seeds), and transplanted on April 20th; Seeds were soaked in clear water on the 28th, sowed on July 1 (dry seeds 90g/pan), and transplanted on July 21; the delayed sowing experiment of late rice was soaked on July 16, sowed on July 19, and transplanted on August 8. The density of machine transplanting is 30cm×11cm, and 100m ² of seedlings are transplanted for each treatment, without repetition. The fertilization amount of each treatment was the same, the amount of nitrogen (N) applied to early rice was 180kg/hm ² , and the amount of nitrogen (N) applied to late rice was 210kg/hm ² , according to the ratio of N:P ₂ O ₅ :K ₂ O=1:0.5:1 Phosphate fertilizer (superphosphate, containing P ₂ O ₅ 12%) and potassium fertilizer (potassium chloride, containing K ₂ O 60%), nitrogen fertilizer (urea, containing 46.4% pure nitrogen) according to the ratio of base fertilizer, tiller fertilizer and ear fertilizer 5 : 3: 2 application, all phosphate fertilizer is applied as base fertilizer at one time, and potassium fertilizer is applied according to the ratio of base fertilizer to tillering fertilizer 6: 4. Other management was carried out in accordance with local high-yielding habits.

Measurement items and methods:

Leaf area and dry matter accumulation: Investigate the leaf area and dry matter weight in the key growth stages (tillering full stage, booting stage, full heading stage, milk ripening stage and mature stage), take 6 replicate samples for each large area, and each replicate 3 holes, the leaf area was measured by the length-width coefficient method, and the leaves, stems, ears and other parts were bagged, killed at 105 °C for 30 minutes, dried at 80 °C to constant weight, and the dry weight of each part was weighed.

Yield and its constituent factors: 3 points were selected diagonally in each large area during the mature stage, and the effective panicle number of continuous 60-foot rice was investigated at each point, and the effective panicle number of a single hole was calculated. Go back to the laboratory to investigate the number of grains per panicle, seed setting rate and thousand-grain weight. Each region randomly harvested 6m ² (repeat every 2m ² ), harvested and dried separately, and converted the actual output according to the moisture content of 13.5%.

Physiological characteristics: The seedlings of each treatment were collected at the three-leaf-one-heart stage, and the antioxidant enzyme activity was measured referring to "Principles and Techniques of Plant Physiological and Biochemical Experiments" edited by Li Hesheng. Peroxidase (POD) activity was measured by the guaiacol method, and the change value of OD470 of 0.1/min was used as a relative enzyme activity unit U; superoxide dismutase (SOD) activity was measured by nitroblue tetrazolium (NBT) In the photochemical reduction method, 50% inhibition is regarded as a unit of enzyme activity U; in the determination of catalase (CAT) activity, the hydrogen peroxide method is used, and the change value of 0.1/min in OD240 is regarded as a relative enzyme activity unit U.

data processing:

Excel 2010 was used for data statistics, and DPS 7.05 was used for variance analysis.

(1) To study the effects of cold-resistant agents and spraying periods on the dynamics of early rice stems and tillers, the results are shown in Figure 1.

It can be seen from Figure 1 that among cold resistance agent treatments, the highest tiller number of C1 treatment was slightly higher, and the average effective panicle number of C2 treatment was higher. Among the treatments during the spraying period, the highest tiller numbers of D2 and D4 treatments were higher, but most of them were invalid Tillering, the number of effective panicles of each treatment was basically the same. Among the combined treatments, the tillering ability of C1D3 and C3D3 was weaker, and the effective panicles of the D2 treatments were higher. In addition, the number of effective panicles of the C2 treatments was higher than that of C1 and C3. On the whole, the treatment of cold resistance agent C2 and the treatment of D2 during the spraying period had stronger tillering ability, and the final effective panicle number was higher.

(2) To study the effects of anti-cold agents and spraying periods on the physiological characteristics of early rice. The activities of various antioxidant enzymes are shown in Table 1.

Table 1 Effects of anti-cold agents and spraying periods on the antioxidant system of early rice leaves

Note: Different lowercase letters indicate a significant difference at the 0.05 level. The same below.

It can be seen from Table 1 that there is no significant difference in POD activity among the three treatments of anti-cold agents, CAT and SOD activities are at a higher level in C3 treatment, and significantly lower in C1 treatment; in the treatment of spraying period, the SOD activity of D2 treatment is slightly higher. Low, CATPOD activity is very high, D5 treatment POD activity is slightly lower, the other two enzyme activities are higher, D3, D1 treatment all enzyme activities are lower, D4 treatment only CAT activity is higher. In the combined treatment, the activities of various enzymes of C3D2 were at a high level, the activities of C3D5 were low in POD, the activities of CAT and SOD were extremely high, the activities of C2D3, C2D4 and C3D1 were very low, and the rules of other treatments were not obvious . In general, cold resistance agents can improve the antioxidant capacity of rice, and C3>C2>C1, D2 and D5 are better in spraying period, and D3 is worse.

(3) To study the effects of anti-cold agents and spraying periods on dry matter accumulation of early rice, the results are shown in Table 2.

Table 2 Effects of anti-cold agents and spraying periods on dry matter accumulation dynamics in the aboveground part of early rice

It can be seen from Table 2 that among the cold resistance agent treatments, the C3 treatment was at the highest level in each period, and the C2 treatment was higher than the C1 treatment in all periods except at the booting stage, and the difference was significant; in different spraying periods, the The D5 treatment was higher at each stage, the dry matter accumulation rate of the D1 treatment was faster in the first and middle stages, and the level was lower at the milk maturity stage, and the opposite was true for the D2 treatment, which was lower at the peak stage, but the dry matter accumulation rate accelerated at the booting stage. After that, it was at a high level in each period, and the dry matter weight of D4 treatment was lower in each period. Among the combined treatments, the dry matter weight of the C2D2, C3D2, and C2D5 treatments was low in the early stage, but the accumulation speed was accelerated in the middle and late stages, and it remained at a very high level until the mature stage. It is low in the period and high in other periods, and C1D1 is low in all periods. In general, the effect of anti-cold agents on dry matter weight is C3>C2>C1. During the spraying period, the dry matter weight of D2 and D5 treatments is higher.

(4) To study the effect of anti-cold agent and spraying period on the leaf area of early rice, the results are shown in Table 3.

Table 3 Effects of cold resistance agents and spraying periods on early rice leaf area index

It can be seen from Table 3 that among the treatments of cold resistance agents, the LAI of the C3 treatment was the highest at each stage, and the difference was significant, followed by the C2 treatment, but the difference was not significant at the full heading stage and the milk ripening stage; , the D2 treatment was lower in the early and middle stages, but higher in the milk maturity stage, the D3 treatment was lower in the booting stage, but the LAI was higher in other stages, and the D5 treatment was lower in the tillering stage and milk maturity stage, and the LAI was higher in other stages; the combination Among the treatments, the LAI of C1D2, C2D1, and C2D4 was lower at each stage, the LAI of C1D3 and C2D2 was lower at the early and middle stage, but higher at milk maturity stage, C3D2 was lower at tillering stage, and higher at other stages, and C3D3 was at full heading stage LAI was lower and other periods were higher. Generally speaking, the anti-cold agent C3>C2>C1, it is better to treat with D2 during the spraying period.

(5) To study the effects of cold-resistant agents and spraying periods on early rice yield and its components, the results are shown in Table 4.

Table 4 Effects of cold-resistant agents and spraying periods on early rice yield and yield composition

It can be seen from Table 4 that among different cold resistance agents, the yield of C3 treatment is significantly higher than that of other treatments. Compared with C1 and C2 treatments, the yield of C3 treatment is 7.6% and 7.1% higher respectively. Through analysis, it is found that the level of each yield component factor is very high , the levels of yield and yield components of C1 and C2 treatments were relatively consistent, and there was no significant difference. During the spraying period, the yields of D2, D4, and D5 treatments were higher, and the yield of D1 treatment was lower due to the lower grain number per panicle, and the lower seed setting rate of D3 treatment, resulting in lower yields. Among the combined treatments, the C3D2 and C3D5 treatments were the highest, and the effective panicle number per unit area of the C3D2 treatment was low, but the extremely high grain number per panicle and the seed setting rate had a significant compensation effect, and the C3D5 treatment had a high level of each yield component factor; , C3D3 treatment had lower yield, C1D1 treatment was due to the lower level of each yield forming factor, C2D5 and C3D3 treatment had similar reasons, the number of effective panicles per unit area was higher, but the seed setting rate was too low, resulting in a very low yield in the end. Low. In general, cold-resistant agents and spraying periods have a greater impact on the yield of early rice. Among the cold-resistant agents, C3 treatment has the most obvious effect on yield improvement. During the spraying period, D2, D4, and D5 treatments have higher yields.

(6) To study the effects of cold-resistant agents and spraying periods on the physiological characteristics of late rice, the results are shown in Table 5.

Table 5 Effects of anti-cold agents and spraying periods on the antioxidant system of late rice leaves

It can be seen from Table 5 that in the treatment of cold resistance agents, the enzyme activities of C3 treatment are the highest, and the enzyme activities of C1 treatment are the lowest. are lower. Among the combination treatments, the enzyme activities of C3F2 treatment were higher, and the enzyme activities of each combination treatment of C1 and F4 were not high. On the whole, the type of cold resistance agent and spraying period had important effects on the activity of antioxidant enzymes in early rice. Improving the stress resistance of rice shows C3>C2>C1, and F2 is more suitable for the spraying period.

(7) The effects of cold-resistant agents and spraying periods on delayed sowing late rice were studied, and the results are shown in Table 6.

Table 6 Effects of anti-cold agents and spraying time on the antioxidant system of delayed sowing late rice leaves

It can be seen from Table 6 that in the treatment of cold resistance agents, the activity of each enzyme is C3>C2>C1. During the spraying period, the activity of each enzyme in the F1 treatment is the highest, and the F4 treatment is the lowest. The enzyme activity gradually decreases as the spraying period delays the trend of. Among the combined treatments, the enzyme activity of each treatment of C1 was still low, only the SOD activity of the C1F1 and C1F4 treatments was slightly higher, and the enzyme activities of the C3F1, C3F2, and C3F3 treatments were all higher. It can be seen that the type of cold resistance agent and the spraying period have a great impact on the stress resistance of late rice. The ability has a significant impact. The cold-resistant agent for machine-transplanted double-cropping rice in Hunan Province in Example 2 of the present invention has the best effect on improving the stress resistance of late rice, followed by S-induced analyticin, and the worst effect on the short stand.

(8) To study the effects of cold-resistant agents and spraying periods on late rice yield and its components, the results are shown in Table 7.

Table 7 Effects of cold-resistant agents and spraying periods on late rice yield and yield composition

It can be seen from Table 7 that among the cold resistance agent treatments, the yield of C3 treatment is the highest, which is 20.2% higher than that of C1 treatment, and 8.8% higher than that of C2 treatment. This is due to the lower number of grains per panicle. During the spraying period, there were no significant differences in the number of grains per panicle, thousand-grain weight and number of grains per panicle among the treatments. The yield of F2 treatment was the highest, and that of F4 treatment was the lowest because of its lower seed setting rate. Among the combined treatments, the yields of C2F2 and C3F2 were the highest, and the yields of C1F4, C2F4 and C1F1 were all lower. From the perspective of yield composition, the number of grains per panicle and seed setting rate of these two treatments were very low. In general, cold resistance agents and spraying periods have a great impact on yield, C3 treatment has the most significant increase in yield, and F2 is the best spraying period.

(9) To study the effects of cold-resistant agents and spraying periods on the yield and yield composition of late sowing rice, and the results are shown in Table 8.

Table 8 Effects of cold-resistant agents and spraying time on the yield and yield composition of delayed-sowing late rice

It can be seen from Table 8 that the delayed sowing experiment encountered obvious low temperature adversity at the heading and flowering stage. Among the cold resistance treatments, the C3 treatment had the highest yield, and there was no significant difference in the yield between the C1 and C2 treatments. The reason for the lower yield of the two was that the number of grains per panicle and the seed set were lower; the yield of the F1 treatment was the highest, and the F4 treatment was the lowest in different spraying periods , the yield decreased with the delay of spraying period, and there was no significant difference in effective panicle number and thousand-grain weight in each spraying period. The main reason for the yield difference was the huge number of grains per panicle and seed setting rate; The yield of each treatment of C3 was higher, and the yield of F4 treatment was the lowest, which was consistent with the law of physiological characteristics. When encountering low temperature and chilling damage, the anti-cold agent for machine-transplanted double-cropping rice in Hunan Province in Example 2 of the present invention has the best effect on improving the stress resistance of rice, and when the low temperature comes, spraying the anti-cold agent as early as possible can avoid yield loss as much as possible.

In summary, the three enzymes CAT, SOD, and POD are important components of the plant tissue protection enzyme system. When plants encounter adversity, the system balance of protective enzymes is broken, the content of oxygen free radicals increases, and the membrane system will be damaged. SOD can eliminate excessive oxygen free radicals in cells and convert them into hydrogen peroxide, while CAT and POD can decompose excess hydrogen peroxide to protect cells. The present invention is based on the activities of various antioxidant enzymes, combined with the characteristics of early and late rice yield formation, to study the problem of low temperature and chilling damage faced by the production of double-cropping rice in southern Hunan. The results show that: among the three anti-cold agents, the The anti-cold agent for machine-transplanted double-cropping rice in southern Hunan is the most effective in improving the stress resistance of early and late rice, followed by S-abensin, and the effect of paclobutrazol is the worst; The anti-cold agent has the best effect, and the late rice is sprayed at the beginning of earing stage, and its resistance to stress is the strongest.

From the perspective of tillering dynamics, the spraying of anti-cold agent for machine-transplanted double-cropping rice in southern Hunan in Example 2 of the present invention has the most obvious effect on improving the tillering of early rice, the tillering occurs faster, and the final number of tillers is also more. - The elicitor has no obvious effect on improving the tillering ability of rice. It is best to spray on one leaf and one center at the time of spraying, and the effect is the best. From the perspective of dry matter accumulation and leaf area dynamics, the anti-cold agent for machine-transplanted double-cropping rice in southern Hunan in Example 2 of the present invention has the best effect, followed by S-attractin, and the worst in short pier. Or spray cold resistance agent after turning green, which has a more obvious effect on improving dry matter accumulation. The type of cold resistance agent and spraying period have a great impact on rice.

From the perspective of yield components, the cold-resistant agent for machine-transplanted double-cropping rice in southern Hunan in Example 2 of the present invention can coordinate the contradiction between each yield component of early rice better, and each yield component index is all higher, and finally has a great impact on yield. The effect of improving the number of grains per panicle is the best. Compared with the short pier, S-abensin has a more obvious effect on increasing the number of grains per panicle. There is no significant difference in other indicators, and the final yield is higher. During the spraying period, spraying with one leaf wholeheartedly or after turning green can achieve higher yields. From the perspective of late rice yield, the effect of S-antisin in late rice was better than that of dwarf pier. Similar to early rice, S-antisin had a greater effect on increasing the number of grains per panicle, and there was no significant difference in other indicators. For late rice in 2018, the cold-resistant agent for machine-transplanted double-cropping rice in southern Hunan in Example 2 of the present invention has a better yield-increasing effect. Compared with the short pier, the yield has increased by 20.2%, mainly due to the increase in the number of effective panicles and seed setting rate; The application period should be selected at the beginning of earing period, and the yield is the highest. In summary, it can be seen that the anti-cold agent for machine-transplanted double-cropping rice in southern Hunan in Example 2 of the present invention has the largest increase in yield, and the spraying period of the anti-cold agent for early rice should be after one leaf and one heart or after turning green, and the anti-cold agent for late rice should be sprayed The period should be at the beginning of earing period.

The above is only a preferred embodiment of the present invention, it should be pointed out that, for those of ordinary skill in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications can also be made. It should be regarded as the protection scope of the present invention.

Claims (4)

1. The south Hunan mechanically-inserted double-cropping rice cold-resistant agent is characterized by comprising the following raw materials in parts by weight: 1 to 3 parts of zinc sulfate, 1 to 3 parts of manganese sulfate, 1 to 3 parts of ammonium chloride, 0.5 to 1.5 parts of beta-cyclodextrin and 0.5 to 1.5 parts of anti-falling agent.

2. The method for using the south Hunan mechanically-inserted double-cropping rice cold-resistant agent as claimed in claim 1, which is characterized by comprising the following steps:

(1) Mixing the south Hunan mechanical-insert double-cropping rice cold-resistant agent of claim 1 with water to obtain a mixed solution;

(2) Spraying the obtained mixed solution in the early rice one-leaf one-core period or after turning green, and spraying the obtained mixed solution in the late rice spike period.

3. The method of claim 2, wherein the mixing ratio of the south Hunan mechanically inserted double cropping rice cold resistant agent and water in step (1) is 45-55 g:35000-45000 mL.

4. A method of use according to claim 2 or 3, wherein the amount of spraying in step (2) is 35000-45000 mL/mu.

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