CN116026906A - A microfluidic blood glucose detection chip - Google Patents

Abstract

The invention discloses a microfluidic blood glucose detection chip, which includes a blood collection unit, a blood detection unit and a plastic housing, and is characterized in that the blood collection unit includes a negative pressure interface, an annular collection groove, a diversion groove and a collection assembly , the collection assembly includes an injection port, pure water and a rubber plug, the pure water is sealed in a cavity formed by a rubber plug and a plastic shell, the outer part of the rubber plug is connected with the motor ejector rod of a supporting instrument, and the injection port Sealed by a sealing film; the blood detection unit includes a blood-conducting material, an enzyme coating, an insulating layer, a substrate, an electrode, an adsorption tank, a flow channel and an electrode interface, and a water-absorbing material is arranged in the adsorption tank, and the flow channel is arranged Above the adsorption tank; above the substrate, electrodes, insulating layers, enzyme coatings, and blood-conducting materials are layered and stacked from bottom to top; the electrode interface is connected to the electrode thimble of the supporting instrument; the blood-conducting material is connected to the negative pressure The interface is connected. Putting the user's finger on the detection chip can realize the functions of automatic needle-free blood collection, sample loading and blood glucose detection. Sense of fear.

Description

A microfluidic blood glucose detection chip

technical field

The invention relates to the technical field of blood sugar detection equipment, in particular to a microfluidic blood sugar detection chip.

Background technique

Diabetes is a common chronic disease. Patients need to monitor and test their blood sugar frequently. Too high or too low blood sugar will have very adverse effects on the body, and even be life-threatening. The current common blood sugar detection method is mainly to use a blood collection pen and a blood collection needle to puncture the skin of the fingertip, then squeeze out the blood and drop it on the blood sugar test paper, then insert the test paper into the blood sugar tester, and finally the blood sugar tester reads out the data and show. There are three problems in this method. First, the steel needle blood collection pain is more obvious, and the patient has pain every time blood collection, and the psychological fear is very strong; second, the needle of the blood collection pen is very sharp, and it is very easy to pierce other parts of the body. The third is that the manual operation steps are more complicated, requiring patients or medical staff to manually install the blood collection needle on the blood collection pen, puncture the fingertip skin, then drip blood onto the test strip, and then insert it into the detector It is inconvenient to use for patients, especially the elderly. The test strips used in the above conventional methods only have the function of detection, and do not have the functions of blood collection and interference removal.

Refer to Figure 3 for conventional blood glucose test strips. Its structural composition includes 1' substrate, 2' electrode, 3' insulating layer, 4' enzyme coating, 5' blood-conducting material, and 6' cover plate. Usage process: First, you need to put the blood collection needle into the blood collection pen, then use the blood collection pen to puncture the skin of the fingertip, then squeeze out the blood, pick up the test strip, and put the bleeding finger close to the 5th lead of the test strip Blood materials, blood-conducting materials absorb blood and quickly spread to the top of the 4-enzyme coating. At this time, the user inserts the test paper into the blood glucose detection instrument, and the instrument detects the current signal on the test paper due to biochemical reactions, and then performs calculations and displays the results. . Disadvantages of this program:

1. The reagent strips involved in this solution need to manually use blood collection pens and blood collection needles for blood collection, and the steps are complicated;

2. This solution needs to manually bring the blood close to the test strip, and the amount of blood inhalation is not easy to control.

3. This solution uses blood collection needles to directly see the needle, which increases the patient's psychological fear;

4. This solution can directly see the red blood, which has a negative effect on the psychology of blood-sick patients.

Contents of the invention

The purpose of the present invention is to provide a microfluidic blood glucose detection chip to solve the problems raised in the above-mentioned background technology.

In order to achieve the above object, the present invention provides the following technical solutions: a microfluidic blood glucose detection chip, including a blood collection unit, a blood detection unit and a plastic housing, the blood collection unit includes a negative pressure interface, an annular collection tank, a flow guide Groove and collection assembly, the collection assembly includes injection port, pure water and rubber plug, the pure water is sealed in the cavity formed by the rubber plug and the plastic shell, the outer part of the rubber plug and the motor ejector rod of the supporting instrument connected, and the injection port is sealed by a sealing film;

The blood detection unit includes a blood-conducting material, an enzyme coating, an insulating layer, a substrate, an electrode, an adsorption tank, a flow channel, and an electrode interface. The adsorption tank is provided with a water-absorbing material, and the flow channel is arranged above the adsorption tank;

An electrode, an insulating layer, an enzyme coating and a blood-conducting material are layered and stacked above the substrate from bottom to top;

The electrode interface is connected with the electrode thimble of the supporting instrument;

The blood conducting material is connected with the negative pressure interface.

As a further optimization, the pure water is connected to the injection port through a flow channel.

As a further optimization, the annular collection groove is arranged inside the diversion groove to prevent the blood flow from being blocked after being pressed by the skin.

As a further optimization, the adsorption tank is arranged under the flow channel to remove the mixture of pure water and blood after the skin is punctured, so as to prevent the blood sugar concentration from being diluted to affect the detection result.

As a further optimization, a water-absorbing material is arranged in the adsorption tank.

As a further optimization, the enzyme type used in the enzyme coating is glucose oxidase or glucose dehydrogenase.

As a further optimization, the outer side of the plastic housing has a negative pressure port, which is used for negative pressure drainage, so that blood flows out from the puncture hole on the finger and enters the drainage groove.

As a further optimization, the negative pressure interface is provided with an air-permeable but liquid-impermeable filter element to prevent excess blood from entering the negative pressure system.

Beneficial effect

The microfluidic blood glucose detection chip provided by the present invention can realize automatic needle-free blood collection, sample loading and blood glucose detection functions by putting the user's finger on the detection chip. The operation is very simple and the degree of automation is high; when the collection component When the needle-free design is adopted, the pain is small, and the fear of needle-phobic patients is reduced; considering the influence of pure water on the blood concentration when the skin is pierced, the mixture of pure water and blood is absorbed by the adsorption tank and adsorption material, which effectively improves the detection. the accuracy.

Description of drawings

Fig. 1 is a schematic diagram of the positional relationship of the overall structure of the present invention;

Fig. 2 is a schematic structural diagram of an acquisition component in an embodiment of the present invention;

Fig. 3 is a schematic structural diagram of existing equipment in the background technology of the present invention.

reference sign

Blood conduction material 1, negative pressure interface 2, enzyme coating 3, insulating layer 4, substrate 5, electrode 6, adsorption tank 7, flow channel 8, annular collection tank 9, diversion tank 10, injection port 11, pure water 12 And rubber plug 13, electrode interface 14, plastic shell 15.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Referring to Figures 1 and 2, a microfluidic blood glucose detection chip includes a blood collection unit, a blood detection unit, and a plastic housing 15. The blood collection unit includes a negative pressure interface 2, an annular collection groove 9, a flow guide groove 10 and a collection Components, the collection component includes injection port 11, pure water 12 and rubber plug 13, pure water 12 is sealed in the cavity formed by rubber plug 13 and plastic shell 15, the outer part of rubber plug 13 is connected with the motor ejector rod of the supporting instrument, The ejection port 11 is sealed with a sealing film.

The blood detection unit includes a blood-conducting material 1, an enzyme coating 3, an insulating layer 4, a substrate 5, an electrode 6, an adsorption tank 7, a flow channel 8, and an electrode interface 14. The adsorption tank 7 is provided with a water-absorbing material, which includes but not It is limited to hydrophilic materials and water-absorbent cotton; the flow channel 8 is arranged above the adsorption groove 7; the electrode 6, the insulating layer 4, the enzyme coating 3 and the blood-conducting material 1 are layered and stacked on the substrate 5 from bottom to top. 6. Materials include but not limited to metal materials such as conductive ink, copper, and silver. Enzyme types used in the enzyme coating 3 include but are not limited to glucose oxidase, glucose dehydrogenase, etc., and the electrode interface 14 is connected to the electrode thimble of the supporting instrument; The material 1 is connected to the negative pressure port 2. The blood conducting material 1 is a hydrophilic film, including but not limited to fatty alcohol polyoxyethylene ether material. The flow channel 10 prevents the blood flow from being blocked by the skin. There is an adsorption tank 7 under the flow channel 8, which is used to remove the mixture of pure water and blood after the skin is punctured, so as to avoid the dilution of the blood sugar concentration and thus affect the test results. The water-absorbing material in the adsorption tank The absorption capacity is set to only absorb 2-3 drops of a mixture of pure water and blood.

In this embodiment, when the pure water 12 is pressurized by an external instrument, it is ejected from the injection port 11 at a high speed and pierces the skin, thereby realizing the needle-free blood collection function.

In this embodiment, the plastic shell 15 has a negative pressure port 2 outside, which is used for negative pressure drainage, so that blood flows out from the puncture hole on the finger and enters the drainage groove 10 .

In this embodiment, the negative pressure interface 2 is provided with an air-permeable but liquid-impermeable filter element to prevent excess blood from entering the negative pressure system.

Principle of blood collection: when in use, the user puts the detection chip into the supporting instrument, and then places the sterilized finger on the collection component composed of the annular collection tank 9, the diversion tank 10, and the injection port 11, and controls it through the buttons on the instrument. The spring in the instrument hits the piston quickly, so that the pure water 12 is sprayed onto the finger through the injection port 11 at high speed, the high-speed liquid flow pierces the skin, and the outflowing blood passes through the diversion groove 10 under the negative pressure of the negative pressure interface 2 into the annular collection tank 9 and flow channel 8.

Detection principle: There is an adsorption groove 7 under the flow channel 8, which can absorb a part of the blood mixture with pure water that first enters the flow channel. In the blood material 1, the blood rapidly diffuses in it, and enters the enzyme coating 3 for biochemical reaction, and the weak current generated is captured by the electrode 6 and sent to the instrument through the electrode interface 14 for analysis and display.

In the present invention, specific examples have been applied to explain the principles and implementation methods of the present invention, and the descriptions of the above examples are only used to help understand the method of the present invention and its core idea; meanwhile, for those of ordinary skill in the art, according to this The idea of the invention will have changes in the specific implementation and scope of application. To sum up, the contents of this specification should not be construed as limiting the present invention.

Claims (8)

1. The utility model provides a micro-fluidic blood sugar detects chip, includes blood collection unit, blood detecting element and plastics casing (15), its characterized in that: the blood collection unit comprises a negative pressure interface (2), an annular collecting groove (9), a diversion trench (10) and a collection assembly, wherein the collection assembly comprises an injection port (11), pure water (12) and a rubber plug (13), the pure water (12) is sealed in a cavity formed by the rubber plug (13) and a plastic shell (15), the outer side part of the rubber plug (13) is connected with a motor ejector rod of a matched instrument, and the injection port (11) is sealed by a sealing film;

the blood detection unit comprises a blood guide material (1), an enzyme coating (3), an insulating layer (4), a substrate (5), an electrode (6), an adsorption groove (7), a runner (8) and an electrode interface (14), wherein a water absorbing material is arranged in the adsorption groove (7), and the runner (8) is arranged above the adsorption groove (7);

an electrode (6), an insulating layer (4), an enzyme coating (3) and a blood guiding material (1) are arranged above the substrate (5) in a layered and overlapped mode from bottom to top;

the electrode interface (14) is connected with an electrode thimble of a matched instrument;

the blood guiding material (1) is connected with the negative pressure interface (2).

2. The microfluidic blood glucose detection chip according to claim 1, wherein: the pure water (12) is connected with the jet orifice (11) through the flow passage (8).

3. The microfluidic blood glucose detection chip according to claim 1, wherein: the annular collecting groove (9) is arranged on the inner side of the diversion trench (10).

4. The microfluidic blood glucose detection chip according to claim 1, wherein: the adsorption groove (7) is arranged below the flow channel (8).

5. The microfluidic blood glucose detection chip according to claim 4, wherein: the adsorption groove (7) is internally provided with a water absorbing material.

6. The microfluidic blood glucose detection chip according to claim 1, wherein: the enzyme type adopted by the enzyme coating (3) is glucose oxidase or glucose dehydrogenase.

7. The microfluidic blood glucose detection chip according to claim 4, wherein: the outside of the plastic shell (15) is provided with a negative pressure interface (2) which is used for negative pressure drainage, so that blood flows out from a puncture on a finger and enters the diversion trench (10).

8. The microfluidic blood glucose detection chip according to claim 4, wherein: the negative pressure interface (2) is provided with a filter element which is breathable but not permeable to liquid.

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