CN115895865B - A sequencer control system - Google Patents

Abstract

The present disclosure provides a sequencer control system, including: a host computer configured to issue a first control instruction for a monitoring subsystem to a central control subsystem through a first communication interface, and/or to issue a first control instruction to a central control subsystem through a second communication interface. The central control subsystem issues a second control instruction for the sequencing subsystem; the central control subsystem is used to obtain the monitoring data collected by the monitoring subsystem based on the first control instruction when receiving the first control instruction; convert the monitoring data to Send it to the host computer through the first communication interface; and when receiving the second control instruction, send the second control instruction to the sequencing subsystem, receive the communication control data returned by the sequencing subsystem, and pass the communication control data through the second The communication interface is sent to the host computer; the monitoring subsystem is used to monitor at least one sequencing indicator during the sequencing process to obtain monitoring data; the sequencing subsystem is used to perform sequencing operations based on the second control instruction and generate communication control data.

Description

A sequencer control system

Technical field

The present disclosure relates to the field of genetic detection technology, and specifically to a sequencer control system.

Background technique

In the field of genetic testing technology, sequencer control systems are usually used to detect genome sequences. The sequencer control system is a complex system that includes mechanical, electronic, fluid circuits, optics, image processing and other components; usually, while controlling the work of each component, the sequencer control system will monitor the work of each component. The working status during the process to ensure that each component can work stably. Therefore, a stable and reliable sequencer control system is urgently needed.

Contents of the invention

In view of this, the present disclosure provides at least one sequencer control system to improve the stability of the sequencer control system through orderly control of the host computer to the slave computer.

In a first aspect, the present disclosure provides a sequencer control system, including: a host computer, a central control subsystem, a monitoring subsystem and a sequencing subsystem; the central control subsystem is connected to the host computer and the monitoring subsystem respectively. The system is connected to the sequencing subsystem;

The host computer is configured to issue a first control instruction for the monitoring subsystem to the central control subsystem through a first communication interface, and/or to issue a first control instruction to the central control subsystem through a second communication interface. Issue a second control instruction directed to the sequencing subsystem;

The central control subsystem is configured to, when receiving the first control instruction, obtain the monitoring data collected by the monitoring subsystem based on the first control instruction; and pass the monitoring data through the third A communication interface is sent to the host computer; and, when the second control instruction is received, the second control instruction is sent to the sequencing subsystem, and the sequence returned by the sequencing subsystem based on the The second control instruction performs the communication control data generated after the sequencing operation, and sends the communication control data to the host computer through the second communication interface;

The monitoring subsystem is used to monitor at least one sequencing indicator during the sequencing process and obtain monitoring data;

The sequencing subsystem is configured to perform a sequencing operation based on the second control instruction and generate communication control data.

Considering that the amount of data generated during the control process is large, in order to ensure that the monitoring subsystem and the sequencing subsystem do not interfere with each other and the stability of the instrument, the host computer proposed in the embodiment of the present disclosure can download data to the central control subsystem through the first communication interface. Issue a first control instruction for the monitoring subsystem, and/or issue a second control instruction for the sequencing subsystem to the central control subsystem through the second communication interface; at the same time, the monitoring subsystem and the sequencing subsystem only communicate with the central control subsystem The subsystems are connected, so the central control subsystem can perform unified control on each subsystem after receiving the control instructions issued by the host computer, so that each subsystem can work in an orderly manner and improve the stability of the sequencer control system. higher.

In a possible implementation, the monitoring subsystem includes at least one of the following sensors: a sensor for monitoring the status of the reagent box door, a sensor for monitoring the status of the waste buffer box door, Sensors used to monitor whether the chip is placed, sensors used to monitor whether the chip clamp is clamped, sensors used to monitor whether the waste buffer box is placed in place, sensors used to monitor whether the reagent kit is placed in place, sensors used to monitor the reagent kit Sensors for entering the sequencing position, bubble sensors, flow rate sensors, sensors for monitoring the liquid height in the waste buffer box, pressure sensors, sensors for monitoring status information of chips, kits, and waste buffer boxes;

Each sensor in the monitoring subsystem is connected to the central control subsystem through an interface.

Here, when the monitoring subsystem includes at least one sensor, the sequencing indicators during the sequencing process can be monitored in real time through at least one sensor, so as to ensure the stable operation of the sequencer control system; at the same time, each sensor can be configured through an interface respectively. Being connected to the central control subsystem can ensure that when any interface fails, the sensors connected to other interfaces can work normally, improving the stability of the sequencer control system.

In a possible implementation, when acquiring the monitoring data collected by the monitoring subsystem based on the first control 5 instruction, the central control subsystem is used to:

The first control instruction is parsed to obtain first instruction information; wherein the first instruction information is used to indicate the required type of target monitoring data and the target sensor that collects the target monitoring data;

Encode the first instruction information to generate a first target instruction;

0 Based on the first target instruction, control the target interface connected to the target sensor, and then

Receive target monitoring data sent by the target sensor.

In a possible implementation, the sequencing subsystem includes at least one of the following detection modules:

Temperature control module, syringe pump, distribution valve, horizontal control module, vertical control module, light-emitting diode LED module, laser diode LD module;

5. Each detection module in the sequencing subsystem communicates with the central control subsystem through an interface.

system connected.

Here, when the sequencing subsystem includes at least one detection module, each detection module is connected to the central control subsystem through an interface, which can ensure that when any interface fails,

The detection module connected to other interfaces can work normally, improving the stability of the sequencer control system.

In a possible implementation, when issuing the second control instruction to the sequencing subsystem, the central control subsystem is used to:

The second control instruction is parsed to obtain second instruction information; wherein the second instruction information is used to indicate the target detection module to be controlled;

5. Encode the second instruction information to generate a second target instruction;

The second target instruction is issued to the target detection module through a target interface connected to the target detection module.

In a possible implementation, when the target detection module includes a distribution valve, the distribution valve is configured to control the valve of the distribution valve according to the second target in response to receiving the second target instruction. Rotate in the target rotation direction indicated by the instruction until reaching the target position corresponding to the target position information indicated by the second target instruction;

When the target detection module includes a syringe pump, the syringe pump is configured to, in response to receiving the second target instruction, based on at least one of the reagent type, speed information, and volume information indicated by the second target instruction. A kind of information, absorb the target reagent or push out the target reagent.

In a possible implementation, when the target detection module includes a horizontal direction control module, the horizontal direction control module includes a horizontal platform and a control unit;

The control unit is configured to, in response to receiving the second target instruction, control the horizontal platform to move according to the movement direction and/or movement distance indicated by the second target instruction.

In a possible implementation, the sequencing subsystem further includes: a reagent kit moving module and a waste liquid box motor; the reagent kit moving module and the waste liquid box motor are respectively connected to the horizontal direction control module;

When the target detection module includes a horizontal direction control module and a reagent box movement module, the horizontal direction control module is also configured to: in response to receiving the second target instruction, move according to the second target instruction. direction and/or movement distance to control the movement of the kit moving module;

When the target detection module includes a horizontal direction control module and a waste liquid box motor, the horizontal direction control module is further configured to: in response to receiving the second target instruction, move according to the second target instruction. The direction and/or moving distance controls the rotation of the waste liquid box motor to drive the waste liquid box to move.

In a possible implementation, when the target detection module includes an LD module, the LD module is configured to control the LD light source in the LD module to emit laser in response to receiving the second target instruction.

In a possible implementation, the sequencing subsystem further includes a sequencer camera, and the sequencer camera is connected to the vertical direction control module;

When the target detection module includes a vertical direction control module, the vertical direction control module is configured to perform at least one of the following operations in response to receiving the second target instruction:

Control the motor of the sequencer camera to move to drive the lens of the sequencer camera to move to a target position corresponding to the target position information indicated by the second target instruction; wherein the target position includes: the target position includes: sequencing The position where the accuracy of the sequencer camera matches the preset accuracy, and/or the position where the sequencer camera is focused;

Control the power supply of the vertical direction control module to be disconnected.

In a possible implementation, when the target detection module includes an LED module, the LED module is configured to respond to receiving the second target instruction, based on the light intensity information indicated by the second target instruction and /or color information to control the LED light source in the LED module to emit light beams.

In a possible implementation, when the target detection module includes a temperature control module, the temperature control module is configured to adjust the ambient temperature to the second target command in response to receiving the second target command. Indicated target temperature.

In order to make the above-mentioned objects, features and advantages of the present disclosure more obvious and understandable, preferred embodiments are given below and described in detail with reference to the accompanying drawings.

Description of the drawings

In order to explain the technical solutions of the embodiments of the present disclosure more clearly, the drawings required to be used in the embodiments will be briefly introduced below. The drawings here are incorporated into the specification and constitute a part of this specification. These drawings are The drawings illustrate embodiments consistent with the present disclosure and, together with the description, serve to explain the technical solutions of the present disclosure. It should be understood that the following drawings only illustrate certain embodiments of the present disclosure, and therefore should not be regarded as limiting the scope. For those of ordinary skill in the art, without exerting creative efforts, they can also Other relevant drawings are obtained based on these drawings.

Figure 1 shows a schematic architectural diagram of a sequencer control system provided by an embodiment of the present disclosure;

Figure 2 shows a schematic architectural diagram of a monitoring subsystem in a sequencer control system provided by an embodiment of the present disclosure;

Figure 3 shows a schematic architectural diagram of a sequencing subsystem in a sequencer control system provided by an embodiment of the present disclosure;

Figure 4 shows a schematic architectural diagram of another sequencing subsystem in a sequencer control system provided by an embodiment of the present disclosure.

Detailed ways

In order to make the purpose, technical solutions and advantages of the embodiments of the present disclosure clearer, the technical solutions in the embodiments of the present disclosure will be clearly and completely described below in conjunction with the drawings in the embodiments of the present disclosure. Obviously, the described embodiments These are only some of the embodiments of the present disclosure, but not all of them. The components of the embodiments of the present disclosure generally described and illustrated in the figures herein may be arranged and designed in a variety of different configurations. Therefore, the following detailed description of the embodiments of the disclosure provided in the appended drawings is not intended to limit the scope of the claimed disclosure, but rather to represent selected embodiments of the disclosure. Based on the embodiments of the present disclosure, all other embodiments obtained by those skilled in the art without any creative efforts shall fall within the scope of protection of the present disclosure.

In the field of genetic testing technology, sequencer control systems are usually used to detect genome sequences. The sequencer control system is a complex system that includes mechanical, electronic, fluid circuits, optics, image processing and other components; usually, the host computer in the sequencer control system will monitor each subsystem while controlling its work. The working status of the subsystem during the working process to ensure that the sequencer control system can work stably.

Generally, in a sequencer control system, the host computer usually issues instructions for controlling the work of each subsystem and instructions for obtaining the working status of each subsystem through only one communication interface. This communication method will make each subsystem The coordination of subsystems is disordered, reducing the stability of the sequencer control system. In order to alleviate the above problems, embodiments of the present disclosure propose a sequencer control system.

It should be noted that similar reference numerals and letters represent similar items in the following figures, therefore, once an item is defined in one figure, it does not need further definition and explanation in subsequent figures.

Refer to Figure 1, which is a schematic architectural diagram of a sequencer control system provided by an embodiment of the present disclosure. The sequencer control system includes: a host computer 11, a central control subsystem 12, a monitoring subsystem 13 and a sequencing subsystem 14; a central The control subsystem 12 is connected to the host computer 11, the monitoring subsystem 13 and the sequencing subsystem 14 respectively.

The host computer 11 is configured to issue first control instructions for the monitoring subsystem 13 to the central control subsystem 12 through the first communication interface, and/or issue instructions for the sequencing subsystem to the central control subsystem 12 through the second communication interface. Second control instructions for system 14.

The central control subsystem 12 is configured to, when receiving the first control instruction, obtain the monitoring data collected by the monitoring subsystem 13 based on the first control instruction; and send the monitoring data to the host computer 11 through the first communication interface; and , when receiving the second control instruction, send the second control instruction to the sequencing subsystem 14, receive the communication control data generated after the sequencing operation based on the second control instruction returned by the sequencing subsystem 14, and send the communication control data to Sent to the host computer 11 through the second communication interface.

The monitoring subsystem 13 is used to monitor at least one sequencing indicator during the sequencing process and obtain monitoring data.

The sequencing subsystem 14 is used to perform sequencing operations based on the second control instruction and generate communication control data.

During implementation, the sequencer control system may include a host computer 11, a central control subsystem 12, a monitoring subsystem 13 and a sequencing subsystem 14; among which, the host computer 11 is connected to the central control subsystem 12, and the central control subsystem 12 is connected to The monitoring subsystem 13 and the sequencing subsystem 14 are connected. Here, the host computer 11 can be a personal computer (PC), a host computer, or other computer that can directly issue control commands.

During specific implementation, the host computer 11 can issue the first control instruction for the monitoring subsystem 13 to the central control subsystem 12 through the first communication interface; or, the host computer 11 can also issue the first control instruction to the central control subsystem 12 through the second communication interface. Issue the second control instruction for the sequencing subsystem 14; alternatively, the host computer 11 can also issue the first control instruction for the monitoring subsystem 13 to the central control subsystem 12 through the first communication interface, and issue the first control instruction for the monitoring subsystem 13 through the second communication interface. Issue a second control instruction for the sequencing subsystem 14 to the central control subsystem 12 . Wherein, the first communication interface and the second communication interface are not connected.

When receiving the second control instruction, the central control subsystem 12 can issue the second control instruction to the sequencing subsystem 14; the sequencing subsystem 14 can perform a sequencing operation based on the second control instruction, generate and return communication control data; and then , the central control subsystem 12 can receive the communication control data generated after the sequencing operation based on the second control instruction returned by the sequencing subsystem 14, and send the communication control data to the host computer 11 through the second communication interface.

The monitoring subsystem 13 can monitor at least one sequencing indicator during the sequencing process to obtain monitoring data; for example, the sequencing indicators can include the presence of the sample chip to be detected, the liquid level of the waste liquid, the flow rate of the liquid, etc. When receiving the first control instruction, the central control subsystem 12 can also obtain the monitoring data collected by the monitoring subsystem 13 based on the first control instruction; and send the monitoring data to the host computer 11 through the first communication interface.

During specific implementation, the central control subsystem 12 may also include indicator lights to indicate the working status of each subsystem; for example, it may include red and yellow indicator lights. When a subsystem fails, the red indicator light may be controlled to turn on, or the red indicator light may be controlled to turn on. Control the red indicator light to flash; when the subsystem is working normally, you can control the green indicator light to light up, or you can control the green indicator light to flash.

Considering that the amount of data generated during the gene sequencing process is large, in order to ensure that the monitoring subsystem and the sequencing subsystem do not interfere with each other and the stability of the instrument, the host computer proposed in the embodiment of the present disclosure downloads data to the central control subsystem through the first communication interface. Issue a first control instruction for the monitoring subsystem, and/or issue a second control instruction for the sequencing subsystem to the central control subsystem through the second communication interface; at the same time, the monitoring subsystem and the sequencing subsystem only communicate with the central control subsystem The subsystems are connected, so the central control subsystem can perform unified control on each subsystem after receiving the control instructions issued by the host computer, so that each subsystem can work in an orderly manner and improve the stability of the sequencer control system. higher.

In a possible implementation, the monitoring subsystem 13 includes at least one of the following sensors: a sensor for monitoring the status of the reagent box door, a sensor for monitoring the status of the waste buffer box door, A sensor that monitors whether the chip is placed, a sensor that monitors whether the chip clamp is clamped, a sensor that monitors whether the waste buffer box is in place, a sensor that monitors whether the reagent kit is in place, a sensor that monitors whether the reagent kit is in place Sensors that enter the sequencing position, bubble sensors, flow rate sensors, sensors used to monitor the liquid height in the waste buffer box, pressure sensors, sensors used to monitor status information of chips, kits, and waste buffer boxes; monitoring Each sensor in the subsystem 13 is connected to the central control subsystem 12 through an interface.

When implemented, the monitoring subsystem 13 may include at least one sensor. For example, as shown in FIG. 2 , it may include a first sensor 131 for monitoring the status of the kit door. The first sensor 131 may interface with The central control subsystem 12 is connected; specifically, it can monitor whether the kit door is open.

A second sensor 132 for monitoring the status of the waste buffer box door may also be included. The second sensor 132 may be connected to the central control subsystem 12 through an interface; specifically, it may monitor whether the waste buffer box door is is open; if it is open, buffer can be obtained or waste can be drained into the waste box.

A third sensor 133 for monitoring whether the chip is inserted may also be included, and the third sensor 133 may be connected to the central control subsystem 12 through an interface.

A fourth sensor 134 for monitoring whether the chip clamp is clamped may also be included, and the fourth sensor 134 may be connected to the central control subsystem 12 through an interface.

A fifth sensor 135 for monitoring whether the waste liquid buffer box is placed in place may also be included, and the fifth sensor 135 may be connected to the central control subsystem 12 through an interface.

A sixth sensor 136 for monitoring whether the test kit is placed in place may also be included, and the sixth sensor 136 may be connected to the central control subsystem 12 through an interface.

A seventh sensor 137 for monitoring whether the reagent kit enters the sequencing position may also be included, and the seventh sensor 137 may be connected to the central control subsystem 12 through an interface.

A bubble sensor 138 may also be included, and the bubble sensor 138 may be connected to the central control subsystem 12 through an interface; specifically, the volume and number of bubbles in the liquid path may be monitored.

A flow rate sensor 139 may also be included, and the flow rate sensor 139 may be connected to the central control subsystem 12 through an interface; specifically, the flow rate of the liquid in the liquid path may be monitored.

An eighth sensor 1310 for monitoring the liquid level in the waste liquid buffer box may also be included, and the eighth sensor 1310 may be connected to the central control subsystem 12 through an interface.

It can also include a pressure sensor 1311, which can be connected to the central control subsystem 12 through an interface; specifically, it can monitor the pressure state of the liquid circuit, and further determine whether there is leakage, blockage, etc. in the liquid circuit.

It may also include a ninth sensor 1312 for monitoring the status information of the chip, the test kit, and the waste buffer box. The ninth sensor 1312 may be a radio frequency card reader, etc., and may be connected to the central control subsystem 12 through an interface; specifically , the status information can include whether the chip is damaged, that is, whether the chip information can be collected normally, chip model information, chip flux information, etc.; it can also include the type information, model information, whether it has been used, production date, and validity period of the kit. etc.; it can also include the model information of the waste buffer box, whether it has been used, production date, expiration date, etc.

During specific implementation, it may also include a temperature sensor, a humidity sensor, etc., which may be used to monitor the temperature and humidity of the sequencing environment, and may be connected to the central control subsystem 12 through the reserved interface 1 .

Here, when the monitoring subsystem includes at least one sensor, the sequencing indicators during the sequencing process can be monitored in real time through at least one sensor, so as to ensure the stable operation of the sequencer control system; at the same time, each sensor can be configured through an interface respectively. Being connected to the central control subsystem 12 can ensure that when any interface fails, the sensors connected to other interfaces can work normally, thereby improving the stability of the sequencer control system.

In a possible implementation, when acquiring the monitoring data collected by the monitoring subsystem 13 based on the first control instruction, the central control subsystem 12 is used to:

The first control instruction is parsed to obtain first instruction information; wherein the first instruction information is used to indicate the required type of target monitoring data and the target sensor that collects the target monitoring data.

The first instruction information is encoded to generate a first target instruction.

Based on the first target instruction, the target interface connected to the target sensor is controlled to receive target monitoring data sent by the target sensor.

During implementation, after receiving the first control instruction, the central control subsystem 12 can parse the first control instruction to obtain the first instruction information; specifically, the instruction includes information bits and check bits, and can analyze the first control instruction. Decode, and check whether there are errors in the instruction information through check bits and modify it to obtain the first instruction information; wherein the first instruction information can be used to indicate the required type of target monitoring data and the target sensor that collects the target monitoring data. ; For example, if the host computer 11 needs to monitor the liquid flow rate in the liquid path, the first command information may indicate the monitoring data of the liquid flow rate and the flow rate sensor that collects the liquid flow rate. Further, the central control subsystem 12 can encode the first instruction information, generate the first target instruction, and can control the target interface connected to the target sensor based on the first target instruction, and receive the target monitoring data sent by the target sensor; example Specifically, if the interface is in a high-level state, such as 1, data can be received; if the interface is in a low-level state, such as 0, data cannot be received; the central control subsystem 12 can control based on the first target instruction. If the target interface connected to the target sensor is in a high-level state, the target monitoring data sent by the target sensor is received.

In a possible implementation, as shown in Figure 3, the sequencing subsystem 14 includes at least one of the following detection modules: a temperature control module 141, a syringe pump 142, a distribution valve 143, a horizontal direction control module 144, and a vertical direction control module. 145. Light-emitting diode (LED) module 146, laser diode (LD) module 147; each detection module in the sequencing subsystem 14 is connected to the central control subsystem 12 through an interface.

During implementation, the sequencing subsystem 14 may include at least one monitoring module. For example, as shown in FIG. 3 , it may include a temperature control module 141, a syringe pump 142, a distribution valve 143, a horizontal direction control module 144, and a vertical direction control module. 145. LED module 146, LD module 147; among them, the temperature control module 141 can be connected to the central control subsystem 12 through an interface; the syringe pump 142 can be connected to the central control subsystem 12 through an interface; the distribution valve 143 can be connected to the central control system through an interface. The subsystem 12 is connected; the horizontal direction control module 144 can be connected to the central control subsystem 12 through an interface; the vertical direction control module 145 can be connected to the central control subsystem 12 through an interface; the LED module 146 can be connected to the central control subsystem 12 through an interface. Connected; the LD module 147 can be connected to the central control subsystem 12 through an interface.

During specific implementation, other required detection modules may also be included, and other required detection modules may be connected to the central control subsystem 12 through the reserved interface 2 .

Here, when the sequencing subsystem includes at least one detection module, each detection module is connected to the central control subsystem through an interface, which can ensure that when any interface fails, detection connected to other interfaces can be guaranteed. The module can work normally and improve the stability of the sequencer control system.

In a possible implementation, when issuing the second control instruction to the sequencing subsystem 14, the central control subsystem 12 is used to:

The second control instruction is parsed to obtain second instruction information; wherein the second instruction information is used to indicate the target detection module to be controlled.

The second instruction information is encoded to generate a second target instruction.

The second target command is sent to the target detection module through the target interface connected to the target detection module.

During implementation, after receiving the second control instruction, the central control subsystem 12 can parse the second control instruction to obtain the second instruction information; specifically, the instruction includes information bits and check bits, and can analyze the second control instruction. Decode, and check whether there is an error in the instruction information through the check bit and modify it to obtain the second instruction information; wherein the second instruction information can be used to indicate the target detection module to be controlled. Further, the central control subsystem 12 can process the second instruction information.

Line coding is performed to generate a second target command, and the second target command can be sent to the target detection module through the target interface 5 connected to the target detection module.

In a possible implementation, when the target detection module includes a distribution valve 143, the distribution valve 143 is configured to, in response to receiving the second target instruction, control the valve of the distribution valve 143 in accordance with the target rotation direction indicated by the second target instruction. Rotate until it reaches the target position corresponding to the target position information indicated by the second target instruction.

0 When the target detection module includes the syringe pump 142, the syringe pump 142 is used to respond to receiving the

The second target instruction is to absorb the target reagent or push out the target reagent based on at least one of the reagent type, speed information, and volume information indicated by the second target instruction.

During implementation, when the target detection module includes a distribution valve 143, the distribution valve 143 can be used to respond to

In response to receiving the second target command, the valve of the distribution valve 143 is controlled to rotate according to the target rotation direction indicated by the second target command 5, such as clockwise rotation or counterclockwise rotation;

Until the target position corresponding to the target position information indicated by the second target instruction is reached. For example, if the second target instruction indicates that the target rotation direction is clockwise and the target position information is the position of valve port 2, then the valve of the distribution valve 143 is controlled to rotate clockwise until it reaches the position of valve port 2. specific

During implementation, if the second target instruction does not indicate the target rotation direction, 0 rotation will be performed according to the preset rotation direction.

When the target detection module includes the syringe pump 142, the syringe pump 142 may be configured to, in response to receiving the second target instruction, aspirate the target based on at least one of the reagent type, speed information, and volume information indicated by the second target instruction. reagent or push out the target reagent.

During specific implementation, if the second target instruction does not indicate speed information or volume information, the target reagent is sucked or pushed out according to the preset speed or volume of 5. If the second target command does not indicate try

reagent type, then aspirate or eject the target reagent of the preset reagent type, or aspirate or eject the target reagent that matches the last reagent type. Among them, the preset reagent type, preset speed and preset volume can be set according to the actual situation.

For example, when the second target instruction indicates to aspirate a reagent of reagent type A, the syringe pump 142 may aspirate a preset volume of reagent A at a preset speed; or, when the second target instruction indicates to aspirate a preset volume of reagent A at a speed of 0.2 ml per second. When pushing out the reagent of reagent type B, the syringe pump 142 can push out the preset volume of reagent B at a speed of 0.2 ml per second.

In a possible implementation, as shown in FIG. 4 , when the target detection module includes the horizontal direction control module 144 , the horizontal direction control module 144 includes a horizontal platform 1441 and a control unit 1442 .

The control unit 1442 is configured to, in response to receiving the second target instruction, control the horizontal platform 1441 to move according to the movement direction and/or movement distance indicated by the second target instruction.

During implementation, when the target detection module includes the horizontal direction control module 144, the horizontal direction control module 144 may include a horizontal platform 1441 and a control unit 1442; the horizontal platform 1441 may be used to carry the sample chip; the control unit 1442 may be used to respond to receiving The second target instruction controls the horizontal platform 1441 to move according to the movement direction and/or movement distance indicated by the second target instruction, so as to adjust the position of the sample chip photographed by the sequencer camera in the sequencing subsystem 14; wherein the movement direction can be Including forward, backward, left and right.

During specific implementation, if the second target instruction does not indicate a movement direction or a movement distance, the movement is performed according to the preset movement direction or the preset distance. For example, if the movement direction indicated by the second target instruction is to move to the left, then the horizontal platform 1441 is controlled to move a preset distance to the left; if the movement distance indicated by the second target instruction is 5 cm, the horizontal platform 1441 is controlled to move to the preset distance. Move 5 cm in direction. Among them, the preset direction and preset distance can be set according to the actual situation.

In a possible implementation, as shown in Figure 4, the sequencing subsystem 14 also includes: a reagent kit moving module 148 and a waste liquid box motor 149; the reagent kit moving module 148 and the waste liquid box motor 149 are respectively connected with the horizontal direction control module 144 connected.

When the target detection module includes the horizontal direction control module 144 and the reagent box movement module 148, the horizontal direction control module 144 is also used to: in response to receiving the second target instruction, follow the movement direction and/or movement indicated by the second target instruction. distance, the reagent kit moving module 148 is controlled to move.

When the target detection module includes the horizontal direction control module 144 and the waste liquid box motor 149, the horizontal direction control module 144 is also used to: in response to receiving the second target instruction, follow the movement direction and/or movement indicated by the second target instruction. distance to control the rotation of the waste liquid box motor 149 to drive the waste liquid box to move.

During implementation, the sequencing subsystem 14 may also include a reagent kit moving module 148 and a waste liquid box motor 149; the reagent kit moving module 148 and the waste liquid box motor 149 are respectively connected to the horizontal direction control module 144.

When the target detection module includes the horizontal direction control module 144 and the reagent box movement module 148, the horizontal direction control module 144 can also be used to respond to receiving the second target instruction, according to the movement direction and/or movement distance indicated by the second target instruction. , controlling the reagent kit moving module 148 to move. The moving direction may include forward, backward, left, right, up, and down; moving in the horizontal direction can change the position of the reagent box so that the syringe pump 142 can absorb reagents of different reagent types; moving in the vertical direction Movement in the direction allows the syringe pump 142 to pierce the sealing cap of the reagent.

During specific implementation, if the second target instruction does not indicate a movement direction or a movement distance, the movement is performed according to the preset movement direction or the preset distance. For example, if the movement direction indicated by the second target instruction is to move upward, the reagent box moving module 148 is controlled to move upward by a preset distance; if the movement distance indicated by the second target instruction is 5 cm, the reagent box moving module 148 is controlled to move upward. Move 5 cm in the preset direction. Among them, the preset direction and preset distance can be set according to the actual situation.

When the target detection module includes the horizontal direction control module 144 and the waste liquid box motor 149, the horizontal direction control module 144 can also be used to respond to receiving the second target instruction, according to the moving direction and/or moving distance indicated by the second target instruction. , controlling the rotation of the waste liquid box motor 149 to drive the waste liquid box to move. The moving direction may include upward and downward. During specific implementation, if the second target instruction does not indicate a movement direction or a movement distance, the movement is performed according to the preset movement direction or the preset distance.

In a possible implementation, when the target detection module includes the LD module 147, the LD module 147 is configured to control the LD light source in the LD module 147 to emit laser in response to receiving the second target instruction.

During implementation, when the target detection module includes the LD module 147, the LD module 147, in response to receiving the second target instruction, can control the LD light source in the LD module 147 to emit laser to adjust the focus of the sequencer camera in the sequencing subsystem 14. Reference point to speed up sequencer camera focusing.

In a possible implementation, as shown in FIG. 4 , the sequencing subsystem 14 also includes a sequencer camera 1410 , and the sequencer camera 1410 is connected to the vertical direction control module 145 .

When the target detection module includes the vertical direction control module 145, the vertical direction control module 145 is configured to perform at least one of the following operations in response to receiving the second target instruction:

The first operation is to control the motor of the sequencer camera 1410 to move to drive the lens of the sequencer camera 1410 to move to the target position corresponding to the target position information indicated by the second target instruction; wherein the target position includes: the accuracy of the sequencer camera The position that matches the preset accuracy, and/or the position where the sequencer camera is focused.

The second operation is to control the power supply of the vertical direction control module 145 to be turned off.

During implementation, the sequencing subsystem 14 may also include a sequencer camera 1410 , and the sequencer camera 1410 is connected to the vertical direction control module 145 . When the target detection module includes the vertical direction control module 145, the vertical direction control module 145 can be used to control the motor of the sequencer camera 1410 to rotate in response to receiving the second target instruction to drive the lens of the sequencer camera 1410 to move. The second target instruction indicates the target position corresponding to the target position information.

For example, in order to ensure high accuracy of the sequencer camera 1410, after the sequencer camera 1410 is powered on, the lens of the sequencer camera 1410 needs to be moved to the target position; at this time, the target position is the center of the lens of the sequencer camera 1410. The zero point position means that when moving the lens of the sequencer camera 1410 to the target position, the accuracy of the sequencer camera 1410 matches the preset accuracy; or when adjusting the focus of the sequencer camera 1410, the sequencer camera 1410 needs to be moved to the target position. The lens of sequencer camera 1410 moves to the target position; at this time, the target position is the position where sequencer camera 1410 focuses. That is, when the lens of sequencer camera 1410 is moved to the target position, the image captured by sequencer camera 1410 is clearer.

During specific implementation, the vertical direction control module 145 can also be used to cut off the power supply of the vertical direction control module 145 in response to receiving the second target instruction, so as to reduce the loss of the vertical direction control module 145. At the same time, if sequencing If there is a movement error in the lens of the instrument camera 1410, the instrument working stability can be improved by cutting off the power supply of the vertical direction control module 145.

In a possible implementation, when the target detection module includes the LED module 146, the LED module 146 is configured to, in response to receiving the second target instruction, control based on the light intensity information and/or color information indicated by the second target instruction. The LED light source in the LED module 146 emits a light beam.

During implementation, when the target detection module includes the LED module 146, the LED module 146, in response to receiving the second target instruction, can control the LED light source in the LED module 146 to emit a beam, and can stimulate the fluorescence of different markers in the reacted reagents, So that the sequencer camera 1410 in the sequencing subsystem 14 can obtain image information more easily.

In a possible implementation, when the target detection module includes the temperature control module 141, the temperature control module 141 is configured to adjust the ambient temperature to the target temperature indicated by the second target command in response to receiving the second target command.

During implementation, when the target detection module includes the temperature control module 141, the temperature control module 141 can be used to respond to receiving the second target instruction and adjust the ambient temperature to the target temperature indicated by the second target instruction, so as to provide appropriate conditions for the reagent reaction. temperature.

In the several embodiments provided in this disclosure, it should be understood that the disclosed system can be implemented in other ways. The system embodiments described above are only illustrative. For example, the division of the units is only a logical function division. In actual implementation, there may be other division methods. For example, multiple units or components may be combined or can be integrated into another system, or some features can be ignored, or not implemented. On the other hand, the coupling or direct coupling or communication connection between each other shown or discussed may be through some communication interfaces, and the indirect coupling or communication connection of the devices or units may be in electrical, mechanical or other forms.

The units described as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place, or they may be distributed to multiple network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of this embodiment.

In addition, each functional unit in various embodiments of the present disclosure may be integrated into one processing unit, or each unit may exist physically alone, or two or more units may be integrated into one unit.

The above are only specific embodiments of the present disclosure, but the protection scope of the present disclosure is not limited thereto. Any person familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed in the present disclosure, and they should be covered by within the scope of this disclosure. Therefore, the protection scope of the present disclosure should be subject to the protection scope of the claims.

Claims (12)

1. A sequencer control system, comprising: the system comprises an upper computer, a central control subsystem, a monitoring subsystem and a sequencing subsystem; the central control subsystem is respectively connected with the upper computer, the monitoring subsystem and the sequencing subsystem;

The upper computer is used for issuing a first control instruction aiming at the monitoring subsystem to the central control subsystem through a first communication interface and issuing a second control instruction aiming at the sequencing subsystem to the central control subsystem through a second communication interface; wherein the first communication interface is not in communication with the second communication interface;

the central control subsystem is used for acquiring monitoring data acquired by the monitoring subsystem based on the first control instruction when the first control instruction is received; the monitoring data are sent to the upper computer through the first communication interface; when the second control instruction is received, the second control instruction is issued to the sequencing subsystem, communication control data generated after sequencing operation based on the second control instruction and returned by the sequencing subsystem are received, and the communication control data are sent to the upper computer through the second communication interface; the first instruction information obtained by analyzing the first control instruction is used for indicating target monitoring data of a required type and a target sensor which is included in the monitoring subsystem and is used for collecting the target monitoring data, and the second instruction information obtained by analyzing the second control instruction is used for indicating a target detection module to be controlled which is included in the sequencing subsystem;

The monitoring subsystem is used for monitoring at least one sequencing index in the sequencing process to obtain monitoring data;

the sequencing subsystem is used for performing sequencing operation based on the second control instruction and generating communication control data.

2. The sequencer control system of claim 1, wherein the monitoring subsystem comprises at least one of the following sensors: the device comprises a sensor for monitoring the state of a reagent box door, a sensor for monitoring the state of a waste liquid buffer box door, a sensor for monitoring whether a chip is put in, a sensor for monitoring whether a chip clamp is clamped, a sensor for monitoring whether a waste liquid buffer box is put in place, a sensor for monitoring whether a reagent box is put in place, a sensor for monitoring whether the reagent box enters a sequencing position, a bubble sensor, a flow rate sensor, a sensor for monitoring the liquid height in the waste liquid buffer box, a pressure sensor, and a sensor for monitoring the state information of the chip, the reagent box and the waste liquid buffer box;

each sensor in the monitoring subsystem is connected with the central control subsystem through an interface respectively.

3. The sequencer control system according to claim 2, wherein the central control subsystem, when acquiring the monitoring data acquired by the monitoring subsystem based on the first control instruction, is configured to:

analyzing the first control instruction to obtain first instruction information;

encoding the first instruction information to generate a first target instruction;

and controlling a target interface connected with the target sensor based on the first target instruction, and receiving target monitoring data sent by the target sensor.

4. The sequencer control system according to claim 1, wherein said sequencing subsystem comprises at least one detection module of: the device comprises a temperature control module, an injection pump, a distribution valve, a horizontal direction control module, a vertical direction control module, a Light Emitting Diode (LED) module and a Laser Diode (LD) module;

each detection module in the sequencing subsystem is connected with the central control subsystem through an interface respectively.

5. The sequencer control system of claim 4, wherein the central control subsystem, when issuing the second control instruction to the sequencing subsystem, is configured to:

Analyzing the second control instruction to obtain second instruction information;

encoding the second instruction information to generate a second target instruction;

and issuing the second target instruction to the target detection module through a target interface connected with the target detection module.

6. The sequencer control system according to claim 5, wherein when said target detection module comprises a dispensing valve, said dispensing valve is configured to control a valve of said dispensing valve to rotate in a target rotation direction indicated by said second target command in response to receiving said second target command until a target position corresponding to target position information indicated by said second target command is reached;

when the target detection module comprises a syringe pump, the syringe pump is used for responding to the second target instruction and sucking or pushing out a target reagent based on at least one of reagent type, speed information and volume information indicated by the second target instruction.

7. The sequencer control system according to claim 5, wherein when said target detection module comprises a horizontal direction control module, said horizontal direction control module comprises a horizontal platform and a control unit;

And the control unit is used for responding to the received second target instruction and controlling the horizontal platform to move according to the moving direction and/or the moving distance indicated by the second target instruction.

8. The sequencer control system according to claim 5, wherein said sequencing subsystem further comprises: a reagent box moving module and a waste liquid box motor; the reagent box moving module and the waste liquid box motor are respectively connected with the horizontal direction control module;

when the target detection module comprises a horizontal direction control module and a reagent box moving module, the horizontal direction control module is further used for: responding to the second target instruction, and controlling the reagent box moving module to move according to the moving direction and/or the moving distance indicated by the second target instruction;

when the target detection module comprises a horizontal direction control module and a waste liquid box motor, the horizontal direction control module is further used for: and responding to the second target instruction, and controlling the waste liquid box motor to rotate according to the moving direction and/or the moving distance indicated by the second target instruction so as to drive the waste liquid box to move.

9. The sequencer control system according to claim 5, wherein when said target detection module comprises an LD module, said LD module is configured to control an LD light source in an LD module to emit laser light in response to receiving said second target instruction.

10. The sequencer control system of claim 5, wherein the sequencing subsystem further comprises a sequencer camera, the sequencer camera being coupled to the vertical direction control module;

when the target detection module includes a vertical direction control module, the vertical direction control module is configured to perform at least one of the following operations in response to receiving the second target instruction:

controlling a motor of the sequencer camera to move so as to drive a lens of the sequencer camera to move to a target position corresponding to the target position information indicated by the second target instruction; wherein the target location comprises: the target location includes: the position where the accuracy of the sequencer camera is matched with the preset accuracy and/or the position where the sequencer camera focuses;

and controlling the power supply of the vertical direction control module to be disconnected.

11. The sequencer control system according to claim 5, wherein when said target detection module comprises an LED module, said LED module is configured to control an LED light source in the LED module to emit a light beam based on illumination intensity information and/or color information indicated by said second target instruction in response to receiving said second target instruction.

12. The sequencer control system of claim 5, wherein when the target detection module comprises a temperature control module, the temperature control module is configured to adjust an ambient temperature to a target temperature indicated by the second target command in response to receiving the second target command.