CN115872812A - Combination of compound microbial fertilizer - Google Patents
Combination of compound microbial fertilizer Download PDFInfo
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- CN115872812A CN115872812A CN202211531389.3A CN202211531389A CN115872812A CN 115872812 A CN115872812 A CN 115872812A CN 202211531389 A CN202211531389 A CN 202211531389A CN 115872812 A CN115872812 A CN 115872812A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 47
- 230000000813 microbial effect Effects 0.000 title claims abstract description 40
- 150000001875 compounds Chemical class 0.000 title claims abstract description 32
- 239000002689 soil Substances 0.000 claims abstract description 42
- 239000000203 mixture Substances 0.000 claims abstract description 32
- 238000012216 screening Methods 0.000 claims abstract description 26
- 238000012360 testing method Methods 0.000 claims abstract description 25
- 201000010099 disease Diseases 0.000 claims abstract description 21
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 21
- 239000003516 soil conditioner Substances 0.000 claims abstract description 17
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 11
- 239000002699 waste material Substances 0.000 claims abstract description 10
- 239000010902 straw Substances 0.000 claims abstract description 9
- 239000000411 inducer Substances 0.000 claims abstract description 8
- 239000012681 biocontrol agent Substances 0.000 claims abstract description 7
- 239000005416 organic matter Substances 0.000 claims abstract description 7
- 239000011368 organic material Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 235000010855 food raising agent Nutrition 0.000 claims abstract description 3
- 238000004519 manufacturing process Methods 0.000 claims abstract description 3
- 230000000694 effects Effects 0.000 claims description 13
- 241000233866 Fungi Species 0.000 claims description 12
- 238000009472 formulation Methods 0.000 claims description 12
- 230000002265 prevention Effects 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 3
- 238000001139 pH measurement Methods 0.000 claims description 3
- 238000011835 investigation Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 description 8
- 230000009286 beneficial effect Effects 0.000 description 8
- 230000035558 fertility Effects 0.000 description 8
- 244000005700 microbiome Species 0.000 description 7
- 239000000126 substance Substances 0.000 description 6
- 239000003607 modifier Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 2
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000002075 main ingredient Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000012956 testing procedure Methods 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 238000013494 PH determination Methods 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002364 soil amendment Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention provides a compound microbial fertilizer, and relates to the technical field of fertilizers. The combination of the compound microbial fertilizer specifically comprises the following steps: s1, screening main formula types of soil conditioners, selecting biocontrol agents and resistance inducers with potential for controlling clubroot diseases on the basis of screening results of early biocontrol agents to develop and improve the formula of the soil conditioner special for clubroot diseases, S2, fermenting an organic matter carrier, fully and uniformly mixing waste mushroom sticks and crop straws for producing edible mushrooms, adding water to the mixture until the mixture is wet, fully fermenting and decomposing the mixture again by using an organic material leavening agent for later use, and S3, performing a matched screening test of the soil conditioner special for clubroot diseases, and adding the soil conditioner into diseased soil according to matched groups in an orthogonal table for fully and uniformly mixing. The invention provides the composition of the compound microbial fertilizer, the optimal proportion of each component in each composition is determined through experiments, the performances of each aspect of the microbial fertilizer are improved, and the compound microbial fertilizer is suitable for mass production and use.
Description
Technical Field
The invention relates to the technical field of fertilizers, in particular to a compound microbial fertilizer.
Background
The compound microbial fertilizer contains more than ten kinds of efficient and active beneficial microbial bacteria, is suitable for various crops, can activate nutrients, improves the nutrient utilization rate, has universality, breaks through the inherent weaknesses of 'specificity', 'limitation' and 'special fertilizer' of common biological fertilizers, is incomparable to other biological fertilizers, can be suitable for various types of soil, generally speaking, all lands with plant growth can be applied with the microbial fertilizer to improve the soil and reduce the use of chemical fertilizers so as to promote the growth of the crops, is beneficial to returning the soil to the natural state and balancing the pH value of the soil to the state required by the crops, all of the microbial fertilizers are used for helping to improve the soil fertility and eliminate the pollution in the soil, water and the atmosphere, and the prior excessive use of chemical fertilizers and pesticides in China not only causes a series of ecological environment pollution, but also seriously harms the sustainable health of people and the sustainable development of agriculture and society, is a green and ecological fertilizer, has the characteristics of improving the fertility, improving the soil fertility, improving the quality, purifying and repairing the soil, reducing the diseases of the plant and improving the food safety, and is an important approach for realizing the sustainable development of agricultural production increase.
Soil mainly comprises three major parts of mineral substances, organic matters and microorganisms, the activity of the microorganisms of a soil micro-ecological system is very important for the nutrition of plant roots, as the beneficial microorganisms in the soil directly participate in the formation of soil fertility, including the conversion of substances and energy in the soil, the formation and decomposition of humus, the release of nutrients and the fixation of nitrogen, but the quantity of the beneficial microorganisms is insufficient in a pure natural state, the acting force is limited, the existing compound microbial fertilizer is mostly composed of one or more microorganisms and other various organic and inorganic substances, but the proportion of the components in the compound microbial fertilizer is not clear statistics, so that the fertilizer contains few beneficial microorganisms and has poor fertilizer efficiency.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the composition of the compound microbial fertilizer, and solves the problems that the raw materials required to be produced are too expensive, the proportion of each component of the composition is not clearly controlled, the quantity of beneficial microorganisms contained in the fertilizer is small, the fertilizer efficiency is poor, and the pH value of soil cannot be changed.
(II) technical scheme
In order to realize the purpose, the invention is realized by the following technical scheme: the composition of the compound microbial fertilizer specifically comprises the following steps:
s1, screening main formula types of soil conditioner
On the basis of the screening result of the early-stage biocontrol agent, a biocontrol agent and an inducer with potential for controlling clubroot disease are selected to develop and improve the formula of the soil conditioner special for clubroot disease;
s2, fermenting organic matter carrier
Fully and uniformly mixing the waste mushroom sticks from the production of edible mushrooms and crop straws, adding water to moisten the mushroom sticks, and then fully fermenting and decomposing the mushroom sticks by using an organic material leavening agent again for later use;
s3. Screening test for compounding soil conditioner special for clubroot
Adding the mixture into disease soil according to the composition in the orthogonal table, fully mixing the mixture, transplanting seedlings into a flowerpot, carrying out investigation on disease incidence and disease index 45 days after transplanting, calculating prevention and control effects through blank control, carrying out orthogonal test statistics by using DPS analysis software, screening out the optimal composition, simultaneously measuring the pH of each treatment, and evaluating whether the soil conditioner has influence on the pH value of the soil.
Preferably, 5 main materials of a suitable organic matter carrier, bacteria, fungi, a resistance inducer and an acid soil conditioner are mainly selected for formula screening in the S1.
Preferably, the mass ratio of the fungus sticks produced by producing the edible fungus waste in the S2 to the crop straws is 1:1.
preferably, the tests in S3 are arranged according to an orthogonal table of L1645, and the assembly screening is completed in a pot plant.
Preferably, when the seedlings are transplanted in the flowerpot in the S3, 10 seedlings are transplanted in each pot.
Preferably, when the combination screening is carried out in the potted plant in the S3, 7000g of diseased soil is contained in each pot.
Preferably, after the composition in the S3 is added into the diseased soil and fully mixed, the soil humidity should be controlled at 30%.
Preferably, the pH determination for each treatment in S3 is repeated 4 times and is completely randomized.
(III) advantageous effects
The invention provides a combination of a compound microbial fertilizer. The method has the following beneficial effects:
1. the invention provides a compound microbial fertilizer, compared with the compound microbial fertilizer prepared in the prior art, the compound microbial fertilizer prepared by the compound microbial fertilizer has the advantages that through an orthogonal test of the optimal formula combination of a compound microbial modifier special for clubroot, the compound microbial fertilizer determines that different combinations of treatment have better prevention and control effects on clubroot of Chinese cabbage and have a certain conditioning effect on the pH value of diseased soil, wherein the formula combinations with treatment numbers of 10 and 11 have the most ideal prevention and control effects on clubroot, the prevention effect can reach more than 97%, and the variance analysis shows no significant difference in the prevention effects of the two combinations, so that the 10 th and 11 th combinations are determined to be the optimal formula combination, the soil ecology of nitrogen fixation, carbon fixation, beneficial flora, medium trace elements and the like can be optimized, crops are abundant, and the functions of enhancing effect, reducing cost, increasing yield and the like are achieved.
2. The invention provides a compound microbial fertilizer, compared with the compound microbial fertilizer in the prior art, different combinations of the compound microbial fertilizer have certain improvement on the pH value of diseased soil, but the analysis on the influence on the prevention effect is combined, the influence of a compound microbial modifier on the pH value of the soil has no obvious control correlation on the clubroot disease of Chinese cabbage, the improvement on the prevention effect is caused by the change of the number of microecology and microbial populations in the soil after application, through the test, the optimal formula combination is determined to be an organic fertilizer 3 bacteria 2 fungi 4 inducer 3 soil modifier 1 and an organic fertilizer 3 bacteria 3 fungi 1 inducer 2 soil modifier 4, so that the carbon can be effectively utilized to improve the carbon-nitrogen ratio needed by the microbes in the soil, the microbes can be rapidly propagated, and the linkage promotion effect of soil biological fertility physical fertility and chemical fertility is further caused, and the compound microbial fertilizer can rapidly improve the soil and continuously express the lasting fertility.
Drawings
FIG. 1 is a table of the screening factors for the formulation of soil amendment according to the present invention;
FIG. 2 is a graph showing the results of an L1645 orthogonal test of the formulation of the complex microbial inoculant of the present invention;
FIG. 3 is a table of orthogonal tests of L1645 according to the present invention;
FIG. 4 is a comparison graph of results of orthogonal tests of the modifier formulation screening of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example (b):
as shown in fig. 1 to 4, the embodiment of the present invention provides a combination of a compound microbial fertilizer, which specifically includes the following steps:
s1, screening main formula types of soil conditioner
On the basis of the screening result of the early-stage biocontrol preparation, a biocontrol agent and an inducer which have potential for controlling clubroot disease are selected to develop and improve the formula of the soil conditioner special for the clubroot disease, and through the analysis and summary of the existing results and test results, testers can find the rule of interaction among the microbial fertilizer combinations, the pertinence of the test is improved, the possibility of test deviation is reduced, the workload of the testers needing to perform the test is reduced, and the working efficiency of the process is improved;
s2, fermenting organic matter carrier
The waste edible fungus stick and the crop straw are fully mixed, water is added until the mixture is wet, and then the mixture is fully fermented and decomposed again by using an organic material leaven for standby use, the waste materials of the waste edible fungus stick and the crop straw are recycled, so that the pollution to the environment caused by straw burning and waste fungus stick treatment is favorably reduced, the effects of saving the environment and raw materials are achieved, the organic material is fermented and decomposed, the decomposition of protein, cellulose, hemicellulose and lignin is favorably realized, bacteria and fungi are compounded, and the temperature is raised, the odor is removed, the plant diseases and insect pests are eliminated, weed seeds are eliminated, and the nutrients are improved;
s3, screening test of soil conditioner formulation special for clubroot
Adding the components in an orthogonal table into disease soil, fully mixing the components, transplanting seedlings into a flowerpot, surveying the disease incidence and disease index 45 days after transplanting, calculating the prevention and control effect through blank control, performing orthogonal test statistics by using DPS analysis software, screening out the optimal components, measuring the pH of each treatment, and evaluating whether the soil conditioner influences the pH value of the soil.
The formula screening of 5 main ingredients including suitable organic matter carriers, bacteria, fungi, resistance inducing agents and acid soil conditioners is mainly selected in the S1, so that control over test variables can be achieved through different proportions of the 5 main ingredients by testers, the operation difficulty is reduced, and the mass ratio of the waste edible fungus sticks and crop straws produced in the S2 is 1:1, variable control is facilitated, accuracy of tests is ensured, tests in S3 need to be arranged according to an orthogonal table of L1645, matched stack screening is completed in a potted plant, testing results can be conveniently recorded by testing personnel, the testing procedures can be conveniently mastered by the testing personnel, when seedlings are transplanted in flowerpots in S3, 10 seedlings are transplanted in each pot, when matched stack screening is performed in the potted plant in S3, 7000g of diseased soil is contained in each pot, control over variables in the testing procedures is facilitated, after matched stack is added into the diseased soil in S3 and fully mixed, soil humidity should be controlled to be 30%, pH measurement of each treatment in S3 needs to be repeated for 4 times, and the pH measurement is completely randomly arranged, and accuracy of the testing results can be improved.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The preparation method of the compound microbial fertilizer is characterized by comprising the following steps:
s1, screening main formula types of soil conditioner
On the basis of the screening result of the early-stage biocontrol agent, a biocontrol agent and an inducer with potential for controlling clubroot disease are selected to develop and improve the formula of the soil conditioner special for clubroot disease;
s2, fermenting organic matter carrier
Fully and uniformly mixing the waste mushroom sticks from the production of edible mushrooms and crop straws, adding water to moisten the mushroom sticks, and fully fermenting and decomposing the mushroom sticks again by using an organic material leavening agent for later use;
s3, screening test of soil conditioner formulation special for clubroot
Adding the mixture into disease soil according to the composition in the orthogonal table, fully mixing the mixture, transplanting seedlings into a flowerpot, carrying out investigation on disease incidence and disease index 45 days after transplanting, calculating prevention and control effects through blank control, carrying out orthogonal test statistics by using DPS analysis software, screening out the optimal composition, simultaneously measuring the pH of each treatment, and evaluating whether the soil conditioner has influence on the pH value of the soil.
2. The formulation of a compound microbial fertilizer as claimed in claim 1, wherein: and 5 main materials of a proper organic matter carrier, bacteria, fungi, an inducer and an acid soil conditioner are mainly selected in the S1 for formula screening.
3. The formulation of a compound microbial fertilizer as claimed in claim 1, wherein: the mass ratio of the waste mushroom sticks produced in the step S2 to the crop straws is 1:1.
4. the formulation of a complex microbial fertilizer according to claim 1, wherein: the tests in S3 need to be arranged according to an orthogonal table of L1645, and the assembly screening is completed in the pot culture.
5. The formulation of a compound microbial fertilizer as claimed in claim 1, wherein: and in the S3, when the seedlings are transplanted in flowerpots, 10 seedlings are transplanted in each pot.
6. The formulation of a compound microbial fertilizer as claimed in claim 1, wherein: and in the S3, when the pot culture is subjected to matched stack screening, 7000g of diseased soil is contained in each pot.
7. The formulation of a compound microbial fertilizer as claimed in claim 1, wherein: and after the composition in the S3 is added into the diseased soil and fully mixed, the soil humidity should be controlled at 30%.
8. The formulation of a compound microbial fertilizer as claimed in claim 1, wherein: the pH measurements for each treatment in S3 were repeated 4 times and were completely randomized.
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| CN202211531389.3A CN115872812A (en) | 2022-12-01 | 2022-12-01 | Combination of compound microbial fertilizer |
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| CN202211531389.3A CN115872812A (en) | 2022-12-01 | 2022-12-01 | Combination of compound microbial fertilizer |
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| JP2001046057A (en) * | 1999-08-12 | 2001-02-20 | Suntory Ltd | New bacterium and production of organic material using the bacterium |
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| US20120270735A1 (en) * | 2011-04-22 | 2012-10-25 | Manjukarunambika Kolandasamy | Preparation of pgpr bioformulation for biological control of red root rot disease in tea plants |
| KR20170082021A (en) * | 2016-01-05 | 2017-07-13 | 주식회사 인정씨앤씨 | Soil Microorganism Preparations and Fertilizer Comprizing the Preparations and Muck |
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2022
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