CN115818829B - Pressure-sustaining two-phase anaerobic digestion device assisted by CO2-loving methanogens and use method - Google Patents
Pressure-sustaining two-phase anaerobic digestion device assisted by CO2-loving methanogens and use method Download PDFInfo
- Publication number
- CN115818829B CN115818829B CN202211331627.6A CN202211331627A CN115818829B CN 115818829 B CN115818829 B CN 115818829B CN 202211331627 A CN202211331627 A CN 202211331627A CN 115818829 B CN115818829 B CN 115818829B
- Authority
- CN
- China
- Prior art keywords
- phase
- pressure
- methane
- acid
- producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000029087 digestion Effects 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 32
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 146
- 239000002253 acid Substances 0.000 claims abstract description 98
- 239000010802 sludge Substances 0.000 claims abstract description 44
- 230000000696 methanogenic effect Effects 0.000 claims abstract description 27
- 241000894006 Bacteria Species 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 239000010815 organic waste Substances 0.000 claims abstract description 13
- 230000002053 acidogenic effect Effects 0.000 claims abstract description 11
- 239000007789 gas Substances 0.000 claims description 34
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 16
- 238000007599 discharging Methods 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 10
- 241001074903 Methanobacteria Species 0.000 claims description 9
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims description 8
- 230000014759 maintenance of location Effects 0.000 claims description 8
- 239000011259 mixed solution Substances 0.000 claims description 8
- 239000001257 hydrogen Substances 0.000 claims description 7
- 229910052739 hydrogen Inorganic materials 0.000 claims description 7
- 230000001450 methanotrophic effect Effects 0.000 claims description 7
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 6
- 241001174354 Methanocella Species 0.000 claims description 4
- 229910052786 argon Inorganic materials 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 239000006174 pH buffer Substances 0.000 claims description 4
- SPOMEWBVWWDQBC-UHFFFAOYSA-K tripotassium;dihydrogen phosphate;hydrogen phosphate Chemical compound [K+].[K+].[K+].OP(O)([O-])=O.OP([O-])([O-])=O SPOMEWBVWWDQBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000002912 waste gas Substances 0.000 claims description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000003786 synthesis reaction Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 241000202974 Methanobacterium Species 0.000 claims description 2
- 241000193751 Methanoculleus Species 0.000 claims description 2
- 241000900014 Methanoregula Species 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- LEAHFJQFYSDGGP-UHFFFAOYSA-K trisodium;dihydrogen phosphate;hydrogen phosphate Chemical compound [Na+].[Na+].[Na+].OP(O)([O-])=O.OP([O-])([O-])=O LEAHFJQFYSDGGP-UHFFFAOYSA-K 0.000 claims description 2
- 230000015556 catabolic process Effects 0.000 abstract description 5
- 238000006731 degradation reaction Methods 0.000 abstract description 5
- 239000010865 sewage Substances 0.000 abstract description 4
- 239000005416 organic matter Substances 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 244000005700 microbiome Species 0.000 description 7
- 239000002351 wastewater Substances 0.000 description 7
- 240000003183 Manihot esculenta Species 0.000 description 6
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 6
- 239000000149 chemical water pollutant Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 238000011084 recovery Methods 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- 238000003860 storage Methods 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 125000004122 cyclic group Chemical group 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 230000020477 pH reduction Effects 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000004020 conductor Substances 0.000 description 3
- 230000001079 digestive effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 239000003345 natural gas Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000654 additive Substances 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000006179 pH buffering agent Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000003933 environmental pollution control Methods 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000012621 metal-organic framework Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Treatment Of Sludge (AREA)
Abstract
The invention relates to a pressure-holding two-phase anaerobic digestion device assisted by CO 2 methane bacteria and a use method thereof, wherein the device comprises an acidogenic phase (100) and a methanogenic phase (200); the using method specifically comprises the following steps: organic waste liquid and anaerobic acid sludge are stirred in an acid producing phase (100), the top space of a pressure-resistant reactor cavity (101) of the acid producing phase holds pressure, then acid liquid flows into a methane producing phase (200) to be contacted with the anaerobic methane-producing sludge on a bearing bed layer (209), CO 2 methane bacteria are additionally inoculated into the bearing bed layer (209), the top space of the pressure-resistant reactor cavity (201) of the methane producing phase holds pressure, methane is released in a pulse mode to enable the top space to hold pressure in a stable mode, and digestion liquid is sprayed in a circulating mode. Compared with the prior art, the invention synchronously promotes the anaerobic digestion efficiency and the biogas purification performance, improves the organic matter degradation rate by more than 30 percent, ensures that the methane content in the biogas reaches more than 90 percent, has higher economic, social and environmental benefits, and can be widely used in the fields of sewage treatment and renewable energy sources.
Description
Technical Field
The invention belongs to the technical field of sewage treatment and renewable energy sources, and relates to a pressure-holding two-phase anaerobic digestion device assisted with CO 2 methane bacteria and a use method thereof.
Background
Anaerobic digestion technology, which utilizes microbial metabolic activity to break down organic waste and produce renewable energy methane, is the most cost-effective sustainable organic waste treatment and energy recovery technology, playing an important role in environmental pollution control and energy regeneration.
Due to the slow metabolism of anaerobic microorganisms and the special nature of large ecological niche difference of multi-group anaerobic microorganisms, the problems of low degradation rate of organic matters and low methane recovery rate of the existing anaerobic digestion are common. Therefore, how to improve the degradation efficiency of the anaerobic digestion organic matter and realize the high-quality recovery of methane is an issue of wide attention of anaerobic digestion. At present, the anaerobic digestion microbial ecological niche condition is mainly improved by separating acid production from methane production, the hydrolysis is accelerated by applying pretreatment methods such as electricity, heat, radiation and the like, and the acidification and the methane production are stimulated by adding mineral nutrient elements, functional enzymes and high-compatibility non-biological materials, however, the pretreatment technology has high input energy and high additive cost, and the methane recovery potential in the methane is limited, so that the methane is difficult to be used in high quality (such as a natural gas conveying pipeline). Patent CN113501646a discloses a method for strengthening two-phase anaerobic digestion of sludge based on coupling of catalytic-conductive material, in a two-phase anaerobic digestion system of sludge, zirconium metal organic framework material is added into hydrolysis acidification phase as catalyst to strengthen hydrolysis acidification efficiency of complex organic matters in sludge, and conductive material is added into methanogenesis phase as conductive medium between inter-nutrient microorganisms to improve conversion efficiency of hydrolysis acidification product into methane, thus realizing efficient methane production of complex organic matters in sludge, but the catalytic-conductive material production process related by the patent is complicated and difficult to recycle so as to have secondary pollution risk.
Regulating anaerobic digestion reactor pressure is an important means of directionally regulating and controlling anaerobic digestion metabolic enzyme activity. The anaerobic digestion is utilized to produce gas and raise pressure, which is an effective technical method, but the quick pressure increase and synergy cannot be realized due to the slow pressure increase in the process. Patent US4722741a proposes a method for increasing the dissolution of carbon dioxide by methane addition measures, thereby increasing the content of methane in the product gas, but the quality of the product gas is improved without further methanation of carbon dioxide, and the methanation efficiency cannot be ensured due to the limitation of gas-liquid mass transfer.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a pressure-holding two-phase anaerobic digestion device assisted by CO 2 methane bacteria and a use method thereof.
The aim of the invention can be achieved by the following technical scheme:
The invention provides a pressure-holding two-phase anaerobic digestion device assisted by CO 2 methane bacteria, which comprises an acidogenic phase and a methanogenic phase;
the acid producing phase comprises an acid producing phase pressure-resistant reactor cavity, an acid producing phase temperature control layer is wrapped outside the acid producing phase pressure-resistant reactor cavity, a stirrer is arranged inside the acid producing phase pressure-resistant reactor cavity, an acid producing phase air inlet and outlet pipeline is arranged at the top of the acid producing phase pressure-resistant reactor cavity, a pressure gauge is arranged on the acid producing phase air inlet and outlet pipeline, and an acid producing phase feeding pipeline and an acid producing phase discharging pipeline are arranged on the side face of the acid producing phase pressure-resistant reactor cavity;
The methane-generating phase comprises a methane-generating phase pressure-resistant reactor cavity, a methane-generating phase temperature control layer is wrapped outside the methane-generating phase pressure-resistant reactor cavity, a methane-generating phase air inlet pipeline, a circulating sprayer and a methane-generating phase air outlet pipeline are arranged at the top of the methane-generating phase pressure-resistant reactor cavity, an intelligent air pressure controller is arranged on the methane-generating phase air outlet pipeline, digestion liquid is introduced from the bottom of the methane-generating phase pressure-resistant reactor cavity to spray, the rear end of the methane-generating phase air outlet pipeline is connected with a gas collecting tank, a methane-generating phase feeding pipeline and a methane-generating phase discharging pipeline are arranged on the side face of the methane-generating phase pressure-resistant reactor cavity, a packed bed is adopted in the methane-generating phase pressure-resistant reactor cavity, and a plurality of bearing beds are arranged in the methane-generating phase pressure-resistant reactor cavity along the height direction of the methane-generating phase pressure-resistant reactor cavity;
the acid producing phase discharging pipeline is communicated with the methane producing phase feeding pipeline.
The invention provides a method for using a pressure-holding two-phase anaerobic digestion device assisted by CO 2 methane bacteria, which comprises the following steps: and continuously stirring organic waste liquid entering from an acid-producing phase feeding pipeline and anaerobic acid-producing sludge in an acid-producing phase, accumulating gas in the top space of a cavity of a pressure-resistant reactor of the acid-producing phase, fully contacting acid liquid with anaerobic methane-producing sludge on a bearing bed layer after flowing into the methane-producing phase, additionally inoculating CO 2 methane into the bearing bed layer, accumulating gas in the top space of the cavity of the pressure-resistant reactor of the methane-producing phase, stopping ventilation after pressure stabilization, controlling pulse release of methane to a gas collecting tank by an intelligent gas pressure controller to ensure stable pressure in the top space, and simultaneously circularly spraying digestive juice.
Further, the ratio VS of the acid sludge and the organic waste liquid is (1-3): 1, the initial pH value of the mixed solution of the acid sludge and the organic waste liquid is 6.0-7.0, and the stirring speed is 80-120 rpm.
Further, the gas stored in the top space of the cavity of the acid-producing phase pressure-resistant reactor is nitrogen or argon, and the pressure is 0.3-0.5 MPa.
Further, the ratio of the methanogenic sludge to the acid liquor is VS, COD is (1-5), 1, a sodium hydroxide solution is added into the acid liquor to adjust the pH value to 6.8-7.5, and a pH buffering agent of 20-50 mM is added, wherein the pH buffering agent comprises a dipotassium hydrogen phosphate-potassium dihydrogen phosphate solution or a disodium hydrogen phosphate-sodium dihydrogen phosphate solution.
Further, the methanobacteria of the CO 2 include a pure strain or dominant mixed flora of Methanobacterium, methanoregula, methanoculleus or Methanocella, and the inoculation proportion of the methanobacteria of the CO 2 is 10-40% (VS/VS) of the anaerobic methanogenic sludge.
Further, the gas stored in the top space of the cavity of the methanogenic phase pressure-resistant reactor is hydrogen or hydrogen-containing waste gas, and the hydrogen-containing waste gas comprises synthesis gas or water gas, and the pressure is 0.5-1 MPa.
Further, the speed of the circulating spraying is 0.4-0.5L/(min m 3), and the period is 2-5 min of continuous spraying every 10 min.
Further, the operation temperature is kept to be 35-39 ℃ consistent with the optimal temperature of the anaerobic sludge, the hydraulic retention time of the acid-producing phase is 3-5 days, and the hydraulic retention time of the methane-producing phase is 3-8 days.
Further, the operation temperature is kept to be 53-57 ℃ consistent with the optimal temperature of the anaerobic sludge, the hydraulic retention time of the acid-producing phase is 1-3 days, and the hydraulic retention time of the methane-producing phase is 1-5 days.
Compared with the prior art, the invention has the following advantages:
(1) The device has the basic characteristics of pressure resistance and two-phase separation, is convenient for the load of functional microorganisms by arranging the bearing bed layer, and simultaneously improves gas-liquid mass transfer by the circulating sprayer so as to provide hardware support for improving anaerobic digestion performance;
(2) According to the invention, anaerobic biological enzyme metabolic activity is stimulated and gas-liquid balance characteristics are improved by pressurization, anaerobic microorganism ecological niche conditions are optimized by separation of an acidogenic phase and a methanogenic phase, directional conversion of CO 2 is promoted by colonization of CO 2 methane bacteria, and gas-liquid mass transfer conditions are optimized by spraying and other processes, so that the degradation rate of anaerobically digested organic matters and high-quality recovery of methane are promoted, and by the method, the degradation rate of COD (chemical oxygen demand) in the anaerobic digestion is improved by more than 30%, and the methane content in the methane reaches more than 90%;
(3) The invention does not need pretreatment and additives, does not have secondary pollution risk, has simple operation, simple flow and low input cost;
(4) The invention can be used in the fields of sewage treatment and renewable energy sources, and provides a new path for organic wastewater treatment and energy conversion and utilization.
Drawings
FIG. 1 is a schematic diagram of a pressure-sustaining two-phase anaerobic digestion device supplemented with a methanotrophic CO 2 in an embodiment of the invention.
The figure indicates:
100-acid producing phase, 101-acid producing phase pressure-resistant reactor cavity, 102-acid producing phase temperature control layer, 103-stirrer, 104-acid producing phase inlet and outlet pipeline, 105-pressure gauge, 106-acid producing phase feeding pipeline, 107-acid producing phase discharging pipeline, 200-methane producing phase, 201-methane producing phase pressure-resistant reactor cavity, 202-methane producing phase temperature control layer, 203-methane producing phase inlet pipeline, 204-methane producing phase outlet pipeline, 205-intelligent air pressure controller, 206-methane producing phase feeding pipeline, 207-methane producing phase discharging pipeline, 208-circulation sprayer and 209-bearing bed layer.
Detailed Description
The present invention will be described in detail with reference to specific examples. The present embodiment is implemented on the premise of the technical scheme of the present invention, and a detailed implementation manner and a specific operation process are given, but the protection scope of the present invention is not limited to the following examples.
The equipment used in the following examples is representative of conventional equipment in the art unless otherwise specified; unless otherwise indicated, all reagents used are commercially available or prepared by methods conventional in the art, and all of the following examples, not specifically described, are accomplished by means of conventional experimentation in the art.
A pressure-holding two-phase anaerobic digestion device assisted by methanobacteria of the genus CO 2, which comprises an acidogenic phase 100 and a methanogenic phase 200;
the acid producing phase 100 comprises an acid producing phase pressure-resistant reactor cavity 101, an acid producing phase temperature control layer 102 is wrapped outside the acid producing phase pressure-resistant reactor cavity 101, a stirrer 103 is arranged inside the acid producing phase pressure-resistant reactor cavity, an acid producing phase air inlet and outlet pipeline 104 is arranged at the top of the acid producing phase pressure-resistant reactor cavity, a pressure gauge 105 is arranged on the acid producing phase air inlet and outlet pipeline 104, and an acid producing phase feeding pipeline 106 and an acid producing phase discharging pipeline 107 are arranged on the side face of the acid producing phase pressure-resistant reactor cavity 101;
the methane generating phase 200 comprises a methane generating phase pressure-resistant reactor cavity 201, a methane generating phase temperature control layer 202 is wrapped outside the methane generating phase pressure-resistant reactor cavity 201, a methane generating phase air inlet pipeline 203, a circulating sprayer 208 and a methane generating phase air outlet pipeline 204 are arranged at the top, an intelligent air pressure controller 205 is arranged on the methane generating phase air outlet pipeline 204, the rear end of the methane generating phase air outlet pipeline 204 is connected with a gas collecting tank 210, a methane generating phase feed pipeline 206 and a methane generating phase discharge pipeline 207 are arranged on the side surface of the methane generating phase pressure-resistant reactor cavity 201, the methane generating phase pressure-resistant reactor cavity 201 adopts a packed bed, and a plurality of bearing beds 209 are arranged in the methane generating phase pressure-resistant reactor cavity 201 along the height direction of the methane generating phase pressure-resistant reactor cavity;
The acidogenic phase discharging pipeline 107 is communicated with the methanogenic phase feeding pipeline 206, and the temperature control layer is heated by water bath.
The method for measuring the volatile solid of the sludge comprises the following steps: the method for testing the organic matter content of the municipal sludge in the municipal sewage treatment plant (CJ/T221-2005) is adopted.
The method for measuring COD of the organic wastewater comprises the following steps: and (3) adopting a Hash rapid digestion spectrophotometry, and after digesting the sample on a DRB200 intelligent digestion instrument for two hours at 150 ℃, measuring COD by using a DR6000 visible light spectrophotometer.
The method for measuring methane comprises the following steps: and (3) measuring various gas response values in the biogas by using a gas chromatograph equipped with a thermal conductivity detector by using a AGILENT GC 7890A gas chromatograph, and calculating methane components according to a standard curve.
Example 1:
The application method of the pressure-holding two-phase anaerobic digestion device assisted by the methanobacteria of the 2 th thermophilic carbon dioxide comprises the following steps:
Fully mixing cassava ethanol wastewater and anaerobic acid sludge in an acid-producing phase 100, storing gas in a top space, maintaining the pressure at 0.5 MPa, then flowing acid liquor into the methane-producing phase 200 to fully contact with the anaerobic acid sludge on a bearing bed 209, additionally inoculating CO 2 methanobacteria into the bearing bed 209, storing gas in the top space, maintaining the pressure at 0.5 MPa, releasing methane in a pulse mode to ensure that the top space of the methane-producing phase 200 is maintained at a pressure not higher than 1MPa, and circularly spraying digestive juice at the speed of 0.5L/(min.m 3).
In the embodiment, the concentration of the acid-producing sludge is 15 gVS/L, the concentration of the cassava ethanol wastewater is 12.8 gCOD/L, and the ratio of the acid-producing sludge to the cassava ethanol wastewater is 1.17:1.
In this embodiment, since the source of the acid-producing sludge and the nature of the cassava ethanol wastewater are different, the mixed solution of the acid-producing sludge and the organic waste liquid may be acid or alkaline, which affects the activity of the anaerobic digestion acid-producing microorganism, and the pH value of the mixed solution is adjusted to be 6.5±0.1 by using a hydrochloric acid solution of 2M or a sodium hydroxide solution of 2M.
In this embodiment, the mixed solution of the acid-generating sludge and the organic waste liquid is continuously stirred at a speed of 100 rpm.
In this embodiment, the headspace gas storage of the acid generating phase 100 is high purity (> 99.99%) argon.
In the embodiment, the ratio of the methanogenic sludge to the acid liquor is 1.56:1, because the COD removal of the cassava ethanol wastewater in the acid production phase 100 is almost negligible, and the concentration of the acid liquor is about 12.8 gCOD/L, so that the concentration of the methanogenic sludge is 20 gVS/L.
In this example, 2M of sodium hydroxide solution was added to the acid solution to adjust the pH to 7.2±0.1, and 50 mM of dipotassium hydrogen phosphate-potassium dihydrogen phosphate solution was added as a pH buffer.
In this example, the methanotrophic CO 2 bacteria is a pure strain and the inoculation ratio is 20% of anaerobic methanogenic sludge (VS/VS).
In this embodiment, the gas that is stored in the headspace of the methanogenic phase 200 is high purity (> 99.99%) hydrogen.
In this embodiment, the cyclic spray period is 5 min per 10 min continuous sprays.
In this example, the operating temperature was 37 ℃.
In this example, the acid producing phase 100 hydraulic residence time was 5 days and the methanogenic phase 200 hydraulic residence time was 8 days.
Comparative example 1:
The use of a two-phase anaerobic digestion device differs from example 1 in that no gas storage pressurization in the acidogenic phase 100 and methanogenic phase 200, no additional inoculation of CO 2 methanotrophic bacteria into the support bed 209, and no cyclic spraying are performed.
The COD removal rate of comparative example 1 was 35.7 mg COD/g VS/d and the biogas methane content was 68.2%. Based on the comparison example 1 as a blank control group, the COD removal rate of the cassava ethanol wastewater by the pressure-sustaining two-phase anaerobic digestion method of the example 1 assisted by the pure bacteria of the fungus is 49.2 mg COD/g VS/d, which is improved by 37.9 percent compared with the comparison example 1, the methane content in the biogas reaches 92.3 percent, and the method meets the standard of pipeline natural gas.
Example 2:
The application method of the pressure-holding two-phase anaerobic digestion device assisted by the methanobacteria of the 2 th thermophilic carbon dioxide comprises the following steps:
fully mixing landfill leachate and anaerobic acid sludge in an acid producing phase 100, storing gas in a top space, maintaining the pressure at 0.3 MPa, then flowing acid liquor into the methane producing phase 200 to fully contact the anaerobic acid sludge on a bearing bed 209, additionally inoculating CO 2 methane bacteria into the bearing bed 209, storing gas in the top space, maintaining the pressure at 0.5 MPa, releasing methane in a pulse mode to ensure that the top space of the methane producing phase 200 is maintained at a pressure not higher than 1 MPa, and circularly spraying digestive juice at a speed of 0.4L/(min.m 3).
In the embodiment, the concentration of the acid-producing sludge is 20 gVS/L, the concentration of the landfill leachate is 14.3 gCOD/L, and the ratio of the acid-producing sludge to the landfill leachate is 1.40:1.
In this embodiment, because the source of the acid-producing sludge and the nature of the landfill leachate are different, the mixed solution of the acid-producing sludge and the organic waste liquid may be acid or alkaline, which affects the activity of the acid-producing microorganism in anaerobic digestion, and the pH value of the mixed solution is adjusted to be 6.5±0.1 by using a hydrochloric acid solution of 2M or a sodium hydroxide solution of 2M.
In this embodiment, the mixed solution of the acid-generating sludge and the organic waste liquid is continuously stirred at a speed of 120 rpm.
In this embodiment, the headspace gas storage of the acid generating phase 100 is high purity (> 99.99%) argon.
In the embodiment, the ratio of the methanogenic sludge to the acid liquor is 1.40:1, because the COD removal of the landfill leachate in the acid producing phase 100 is almost negligible, and the concentration of the acid liquor is about 14.3 gCOD/L, so that the concentration of the methanogenic sludge is 20 gVS/L.
In this example, 2M of sodium hydroxide solution was added to the acid solution to adjust the pH to 7.2±0.1, and 50 mM of dipotassium hydrogen phosphate-potassium dihydrogen phosphate solution was added as a pH buffer.
In this example, the methanobacteria CO 2 were mixed populations enriched with H 2 and CO 2, which were identified by diversity analysis to be predominantly Methanocella (69.1%) and were inoculated at a ratio of 20% (VS/VS) of anaerobic methanogenic sludge.
In this embodiment, the gas in the headspace gas storage of the methanogenic phase 200 is synthesis gas (H 2 =61%).
In this embodiment, the cyclic spray period is 5 min per 10 min continuous sprays.
In this example, the operating temperature was 55 ℃.
In this example, the acid producing phase 100 hydraulic residence time was 2 days and the methanogenic phase 200 hydraulic residence time was 3 days.
Comparative example 2:
The method of using the two-phase anaerobic digestion device differs from example 2 in that the gas storage in the acidogenic phase 100 and methanogenic phase 200 is not pressurized, no additional CO 2 methanotrophic bacteria are inoculated into the support bed 209, and no cyclic spraying is performed.
The COD removal rate of comparative example 2 was 123.6 mg COD/g VS/d and the biogas methane content was 64.1%. Based on the comparison example 2 as a blank control group, the COD removal rate of the landfill leachate of the pressure-holding two-phase anaerobic digestion method of the embodiment 2 supplemented with Methanocella dominant mixed bacteria is 86.9 mg COD/g VS/d, compared with the comparison example 2, the removal rate is improved by 42.2%, the methane content in the biological marsh gas reaches 95.9%, and the method meets the standard of pipeline transportation of natural gas.
The previous description of the embodiments is provided to facilitate a person of ordinary skill in the art in order to make and use the present invention. It will be apparent to those skilled in the art that various modifications can be readily made to these embodiments and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments, and those skilled in the art, based on the present disclosure, should make improvements and modifications without departing from the scope of the present invention.
Claims (5)
1. A pressure-sustaining two-phase anaerobic digestion method assisted by methanobacteria with the presence of CO 2, which is characterized in that a pressure-sustaining two-phase anaerobic digestion device assisted by methanobacteria with the presence of CO 2 is used for anaerobic digestion, and the device comprises an acidogenic phase (100) and a methanogenic phase (200);
The acid-producing phase (100) comprises an acid-producing phase pressure-resistant reactor cavity (101), an acid-producing phase temperature control layer (102) is wrapped outside the acid-producing phase pressure-resistant reactor cavity (101), a stirrer (103) is arranged inside the acid-producing phase pressure-resistant reactor cavity, an acid-producing phase air inlet and outlet pipeline (104) is arranged at the top of the acid-producing phase pressure-resistant reactor cavity, a pressure gauge (105) is arranged on the acid-producing phase air inlet and outlet pipeline (104), and an acid-producing phase feeding pipeline (106) and an acid-producing phase discharging pipeline (107) are arranged on the side face of the acid-producing phase pressure-resistant reactor cavity (101);
The methane-generating phase (200) comprises a methane-generating phase pressure-resistant reactor cavity (201), a methane-generating phase temperature control layer (202) is wrapped outside the methane-generating phase pressure-resistant reactor cavity (201), a methane-generating phase air inlet pipeline (203), a circulating sprayer (208) and a methane-generating phase air outlet pipeline (204) are arranged at the top, an intelligent air pressure controller (205) is arranged on the methane-generating phase air outlet pipeline (204), the rear end of the methane-generating phase air outlet pipeline (204) is connected with a gas collecting tank (210), a methane-generating phase feeding pipeline (206) and a methane-generating phase discharging pipeline (207) are arranged on the side face of the methane-generating phase pressure-resistant reactor cavity (201), the methane-generating phase pressure-resistant reactor cavity (201) adopts a packed bed, and a plurality of bearing beds (209) are arranged in the methane-generating phase pressure-resistant reactor cavity (201) along the height direction of the methane-generating phase pressure-resistant reactor cavity;
the acidogenic phase discharging pipeline (107) is communicated with the methanogenic phase feeding pipeline (206);
The method specifically comprises the following steps: organic waste liquid entering from an acid-producing phase feeding pipeline (106) is stirred with anaerobic acid-producing sludge in an acid-producing phase (100), the top space of a pressure-resistant reactor cavity (101) stores air and holds pressure, then acid liquor flows into a methane-producing phase (200) to be contacted with anaerobic methane-producing sludge on a bearing bed layer (209), CO 2 methane bacteria are additionally inoculated into the bearing bed layer (209), the top space of the pressure-resistant reactor cavity (201) stores air and holds pressure, and an intelligent air pressure controller (205) is used for controlling pulse-type release of methane to a gas collecting box (210) to enable the top space to hold pressure in a stable state, and digestion liquor is circularly sprayed;
The ratio VS of the acid sludge and the organic waste liquid is (1-3) COD is 1, the initial pH value of the mixed solution of the acid sludge and the organic waste liquid is 6.0-7.0, and the stirring speed is 80-120 rpm;
The gas stored in the top space of the acid-producing phase pressure-resistant reactor cavity (101) is nitrogen or argon, and the pressure is 0.3-0.5 MPa;
The ratio of the methanogenic sludge to the acid liquor is VS, wherein COD is (1-5), 1, a sodium hydroxide solution is added into the acid liquor to adjust the pH value to 6.8-7.5, and a pH buffer of 20-50 mM is added, wherein the pH buffer comprises a dipotassium hydrogen phosphate-potassium dihydrogen phosphate solution or a disodium hydrogen phosphate-sodium dihydrogen phosphate solution;
The gas stored in the top space of the methanogenic phase pressure-resistant reactor cavity (201) is hydrogen or hydrogen-containing waste gas, and the hydrogen-containing waste gas comprises synthesis gas or water gas, and the pressure is 0.5-1 MPa.
2. The method for pressure-sustaining two-phase anaerobic digestion with CO 2 methanotrophic bacteria according to claim 1, wherein the CO 2 methanotrophic bacteria comprise a pure strain or dominant mixed flora of Methanobacterium, methanoregula, methanoculleus or Methanocella, and the inoculation ratio of the CO 2 methanotrophic bacteria is 10-40% (VS/VS) of the anaerobic methanogenic sludge.
3. The method for pressure-sustaining two-phase anaerobic digestion with CO 2 methane bacteria as claimed in claim 1, wherein the circulating spraying rate is 0.4-0.5L/(min m 3), and the period is 2-5 min continuously spraying every 10 min.
4. The method for pressure-sustaining two-phase anaerobic digestion with CO 2 methane bacteria as claimed in claim 1, wherein the operation temperature is kept to be 35-39 ℃ with the optimal temperature of anaerobic sludge, the hydraulic retention time of the acidogenic phase (100) is 3-5 days, and the hydraulic retention time of the methanogenic phase (200) is 3-8 days.
5. The method for pressure-sustaining two-phase anaerobic digestion with CO 2 methane bacteria as claimed in claim 1, wherein the operation temperature is kept to be 53-57 ℃ with the optimal temperature of anaerobic sludge, the hydraulic retention time of the acidogenic phase (100) is 1-3 days, and the hydraulic retention time of the methanogenic phase (200) is 1-5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211331627.6A CN115818829B (en) | 2022-10-28 | 2022-10-28 | Pressure-sustaining two-phase anaerobic digestion device assisted by CO2-loving methanogens and use method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211331627.6A CN115818829B (en) | 2022-10-28 | 2022-10-28 | Pressure-sustaining two-phase anaerobic digestion device assisted by CO2-loving methanogens and use method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115818829A CN115818829A (en) | 2023-03-21 |
| CN115818829B true CN115818829B (en) | 2024-11-26 |
Family
ID=85525681
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211331627.6A Active CN115818829B (en) | 2022-10-28 | 2022-10-28 | Pressure-sustaining two-phase anaerobic digestion device assisted by CO2-loving methanogens and use method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115818829B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2732681A1 (en) * | 2008-07-31 | 2010-02-04 | William C. Stewart | Three stage, multiple phase anaerobic digestion system and method |
| CN106282243A (en) * | 2015-05-19 | 2017-01-04 | 香港浸会大学 | Improved method and system for producing methane by two-phase anaerobic digestion method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102010017818A1 (en) * | 2010-02-17 | 2011-08-18 | Meissner, Jan A. | Process and plant for the production of CBM (Compressed BioMethane) as greenhouse gas-free fuel |
| WO2012112679A2 (en) * | 2011-02-15 | 2012-08-23 | University Of South Florida | Method and system for treating wastewater and sludges by optimizing sc02 for anaerobic autotrophic microbes |
| FR3110601B1 (en) * | 2020-05-20 | 2022-06-10 | Tma Process | Process for the methanation of hydrogen H2 and carbon dioxide CO2 or hydrogen H2 and carbon monoxide CO for the production of methane CH4 |
| CN112680326A (en) * | 2021-01-29 | 2021-04-20 | 同济大学 | Method and system for improving yield and purity of anaerobic methane of organic solid waste |
| CN113583856B (en) * | 2021-08-18 | 2024-03-19 | 上海交通大学 | Two-phase coupling anaerobic fermentation biogas production and in-situ biogas purification device |
-
2022
- 2022-10-28 CN CN202211331627.6A patent/CN115818829B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2732681A1 (en) * | 2008-07-31 | 2010-02-04 | William C. Stewart | Three stage, multiple phase anaerobic digestion system and method |
| CN106282243A (en) * | 2015-05-19 | 2017-01-04 | 香港浸会大学 | Improved method and system for producing methane by two-phase anaerobic digestion method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115818829A (en) | 2023-03-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12416025B2 (en) | System for the production of methane from CO2 | |
| Wang et al. | A bench scale study of fermentative hydrogen and methane production from food waste in integrated two-stage process | |
| Peixoto et al. | Hydrogen production from soft-drink wastewater in an upflow anaerobic packed-bed reactor | |
| Guo et al. | Biohydrogen production from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor | |
| Ramos et al. | Anaerobic digestion of vinasse in fluidized bed reactors: Process robustness between two-stage thermophilic-thermophilic and thermophilic-mesophilic systems | |
| CA2655474C (en) | System for the production of methane from co2 | |
| EP2391706B1 (en) | Integrated system for hydrogen and methane production from industrial organic wastes and biomass | |
| Yu et al. | Hydrogen production from rice winery wastewater in an upflow anaerobic reactor by using mixed anaerobic cultures | |
| CN101638670B (en) | Method for co-producing hydrogen and methane by utilizing dry anaerobic fermentation of solid organic wastes | |
| Arooj et al. | Effect of HRT on ASBR converting starch into biological hydrogen | |
| CN103172242B (en) | Method for improving methanogenesis of residual sludge by heat and alkali combined pretreatment | |
| CN103958688A (en) | Method and device for upgrading hydrogen-based biogas | |
| Pokorna et al. | Adaptation of anaerobic culture to bioconversion of carbon dioxide with hydrogen to biomethane | |
| Dinamarca et al. | Apparent hydrogen consumption in acid reactors: observations and implications | |
| Rosa et al. | Review of continuous fermentative hydrogen-producing bioreactors from complex wastewater | |
| CN107555591A (en) | A two-phase anaerobic treatment device and process for direct interspecies electron transfer based on ethanol-type fermentation | |
| CN115818829B (en) | Pressure-sustaining two-phase anaerobic digestion device assisted by CO2-loving methanogens and use method | |
| Zhao et al. | Simultaneous production of hydrogen and volatile fatty acids from anaerobic digestion of Macrocystis pyrifera biomass residues | |
| CN114934077B (en) | A method for enhancing the directional conversion of lactic acid by two-phase anaerobic digestion to produce methane | |
| Poontaweegeratigarn et al. | Hydrogen production from alcohol wastewater by upflow anaerobic sludge blanket reactors under mesophilic temperature | |
| Salehmin et al. | Challenges and mitigation strategies related to biohydrogen production | |
| Cheng et al. | Process recovery of biohydrogenation in a pilot plant from methanogens invasion | |
| Rahma et al. | Biohydrogen production by modified anaerobic fluidized bed reactor (AFBR) using mixed bacterial cultures in thermophilic condition | |
| CN101250555A (en) | A method for continuous wastewater treatment of biological hydrogen production and its special biological hydrogen production fermentation liquid | |
| CN1896258A (en) | Oriented production of acetic acid in sludge anaerobic fermentation by methane-bacterium specific inhibitor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |