CN115814873A - Filter element suction head with adsorption column and using method - Google Patents

Abstract

The invention relates to a filter element suction head with an adsorption column and a using method thereof. The invention takes the suction head of the filter element as a main body, is additionally provided with the micro adsorption column, is tightly attached, is convenient to use, and is suitable for rapidly providing a large batch of high-purity micro samples for nucleic acid or protein sample detection, such as rapid extraction and detection of nucleic acid.

Description

A kind of filter tip with adsorption column and using method

technical field

The invention relates to the related technical field of in vitro diagnosis, in particular to a filter tip with an adsorption column and a use method thereof.

Background technique

The invention mainly relates to the technical field related to in vitro diagnosis, and the suction head is mainly used for the extraction and purification of samples such as nucleic acid and protein.

After a massive search, it was found that the prior art publication number is CN216419454U, which discloses a pre-filtered suction head. There is a first suction hole that communicates with the inner cavity of the main body of the suction head. The opening at the bottom of the main body of the suction head is connected to the tip with two openings through the middle part that is easy to break. The main body, the middle part and the tip of the suction head are integrally formed. Setting, the outer wall and the inner wall of the junction between the middle part and the main body of the suction head are provided with a circle of annular grooves, the inner cavity of the tip part is filled with a rounded truncated filter block, and the first suction hole and the bottom end of the tip part are opened. The apertures of the second suction holes are the same; the utility model provides a suction head which is convenient for pre-filtering before liquid injection.

To sum up, the main function of the adsorption column attached to the existing suction head is to adsorb small inorganic molecules, and its application range is very narrow.

In view of the above-mentioned defects, the designer actively researches and innovates, in order to create a filter tip with an adsorption column and a method of use, so that it has more industrial utilization value.

Contents of the invention

In order to solve the above-mentioned technical problems, the object of the present invention is to provide a filter tip with an adsorption column and a use method thereof.

To achieve the above object, the present invention adopts the following technical solutions:

One of purpose of the present invention:

A filter tip with an adsorption column, comprising a tip body with a conical structure, a liquid inlet hole is arranged on the top of the tip body, a suction hole is arranged on the bottom of the tip body, and a part of the tip body close to the liquid inlet hole A filter element is arranged on one side, and an adsorption column is arranged on the side close to the suction hole in the main body of the suction head.

As a further improvement of the present invention, the thickness of the filter core is 1-2 mm, and the distance between the top of the filter core and the liquid inlet hole is 3-5 mm.

As a further improvement of the present invention, the filter element includes a first filter layer, a second filter layer and a third filter layer in order from bottom to top, and the thickness ratio of the first filter layer, the second filter layer and the third filter layer is 5:3 :2, and the filter apertures of the first filter layer, the second filter layer and the third filter layer become smaller in turn.

As a further improvement of the present invention, the first filter layer is polyethylene terephthalic acid, the second filter layer includes 20%-30% polyethylene and 70%-80% carbon adsorbent, and the third filter layer is polypropylene.

As a further improvement of the present invention, the top of the third filter layer is coated with a coating layer composed of cellulose acetate.

As a further improvement of the present invention, the thickness of the adsorption column is 4-6 mm, and the distance between the bottom of the adsorption column and the suction hole is 8-10 mm.

As a further improvement of the present invention, the diameter of the liquid inlet hole is 0.5-1.5 mm, and the diameter of the suction hole is 4-5.5 mm.

As a further improvement of the present invention, the adsorption column is a nucleic acid adsorption column.

As a further improvement of the present invention, the adsorption column is a protein adsorption column.

Two of the purpose of the present invention:

A method for using a filter tip with an adsorption column, comprising the following steps in sequence:

Step S1, sucking the sample through the suction hole at the bottom of the tip main body, repeating it several times;

Step S2, sucking the cleaning buffer solution through the suction hole at the bottom of the tip main body to pass through the column and discharge it, repeating several times;

Step S3, absorbing the elution buffer through the suction hole at the bottom of the tip main body, discharging it for future use, and repeating it several times;

The elution buffer used in step S3 above is the extracted and purified sample.

As a further improvement of the present invention, the step of aspirating the sample in step S1 is repeated three times, the step of aspirating the washing buffer through the column and discharging it in step S2 is repeated three times, and the step of aspirating the elution buffer in step S3 is repeated three times.

By means of the above solution, the present invention has at least the following advantages:

The present invention takes the filter tip as the main body, and is equipped with a tiny adsorption column, which fits tightly and is easy to use, and is suitable for quickly providing large quantities of high-purity trace samples for nucleic acid or protein sample detection, such as rapid extraction and detection of nucleic acid.

The invention can quickly and conveniently complete the extraction and purification of a large number of nucleic acid and protein samples, greatly increasing the use value of the product in the detection industry of related nucleic acid and protein samples.

The above description is only an overview of the technical solutions of the present invention. In order to understand the technical means of the present invention more clearly and implement them according to the contents of the description, the preferred embodiments of the present invention and accompanying drawings are described in detail below.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention, and thus It should be regarded as a limitation on the scope, and those skilled in the art can also obtain other related drawings based on these drawings without creative work.

Fig. 1 is a structural representation of a filter tip with an adsorption column in the present invention;

Fig. 2 is a schematic structural view of the filter element in Fig. 1;

Fig. 3 is a schematic flow chart of the present invention.

Wherein, the meanings of the reference signs in the figure are as follows.

1 Tip body 2 Filter

3 adsorption column 4 liquid inlet hole

5 Suction hole 6 First filter layer

7 Second filter layer 8 Third filter layer

Detailed ways

The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only Some, but not all, embodiments of the invention. The components of the embodiments of the invention generally described and illustrated in the figures herein may be arranged and designed in a variety of different configurations. Accordingly, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative efforts belong to the protection scope of the present invention.

Example

As shown in Figures 1 to 3,

One of purpose of the present invention:

A filter tip with an adsorption column, comprising a tip body 1 with a conical structure, a liquid inlet hole 4 is provided on the top of the tip body 1, a suction hole 5 is provided at the bottom of the tip body 1, and the tip body 1 A filter element 2 is arranged on a side close to the liquid inlet hole 4 , and an adsorption column 3 is arranged on a side close to the suction hole 5 inside the suction head main body 1 .

The thickness of the filter element 2 is 1-2mm, and the distance between the top of the filter element 2 and the liquid inlet hole 4 is 3-5mm. The filter element 2 includes the first filter layer 6, the second filter layer 7 and the third filter layer 8 from bottom to top, and the thickness ratio of the first filter layer 6, the second filter layer 7 and the third filter layer 8 is 5:3 : 2, and the filter apertures of the first filter layer 6, the second filter layer 7 and the third filter layer 8 become smaller in turn.

The first filter layer 6 is polyethylene terephthalic acid, the second filter layer 7 includes 20%-30% polyethylene and 70%-80% carbon adsorbent, and the third filter layer 8 is polypropylene. The top of the third filter layer 8 is coated with a coating layer composed of cellulose acetate.

Several filter layers can be arranged sequentially along the flow direction of the sample liquid in the tip, the filter aperture of the first filter layer 6 is larger and can be used to filter macromolecular targets, and the second filter layer 7 is used to filter small molecule targets and Adsorbing some active molecules, and then filtering through the third filter layer 8 with a smaller pore size, can delay the speed of the sample liquid passing through the filter element, so that the sample liquid can be fully filtered and adsorbed in the multi-functional filter element to further improve the separation efficiency.

In more embodiments, the number of the second filter layer 7 can be set at least one group or more according to the actual situation, and the second filter layer 7 integrates carbon-based adsorbents or other various functional fillers to carry out various target objects. adsorption. A variety of functional fillers are separated into different second filter layers 7 to prolong the contact time of the sample liquid in the multi-functional filter element and achieve independent adsorption to improve the separation effect.

The adsorption column 3 is a nucleic acid adsorption column or a protein adsorption column, the thickness of the adsorption column 3 is 4-6 mm, and the distance between the bottom of the adsorption column 3 and the suction hole 5 is 8-10 mm.

The diameter of the inlet hole 4 is 0.5-1.5 mm, and the diameter of the suction hole 5 is 4-5.5 mm.

Two of the purpose of the present invention:

A method for using a filter tip with an adsorption column, comprising the following steps in sequence:

Step S1, sucking the sample through the suction hole 5 at the bottom of the tip main body 1, and repeating it several times;

Step S2, absorbing the cleaning buffer solution through the suction hole 5 at the bottom of the tip body 1 to pass through the column and discharge it, repeating several times;

Step S3, absorbing the elution buffer solution through the suction hole 5 at the bottom of the tip main body 1, discharging it for future use, and repeating it several times;

The elution buffer discharged in the above step S3 is the extracted and purified sample, which can be used for subsequent qPCR detection.

Wherein, the step of aspirating the sample in step S1 is repeated three times, the step of aspirating the washing buffer through the column and discharging it in step S2 is repeated three times, and the step of aspirating the elution buffer in step S3 is repeated three times. In addition, according to the requirements of the sample type and testing specifications, the number of repetitions in the above steps should be repeated at least three times.

The present invention is suitable for rapidly providing large quantities and high-purity trace samples for the detection of nucleic acid or protein samples, such as rapid extraction and detection of nucleic acids.

The invention has potential application in the following and more kits:

1. Covid 19 RNA isolation kit;

2. Mouse genotyping kit;

3. Genomic DNA extraction kit;

4. DNA gel extraction kit;

5. Plasmid mini preparation kit;

6. Mini RNA extraction kit;

7. DNA virus (AAV) titration kit.

The purpose of the present invention is to provide a filter tip equipped with a nucleic acid or protein adsorption column, which can quickly and conveniently complete the extraction and purification of nucleic acid and protein samples.

With high-quality zero-residue or extremely low-residue filter tips as the main body, it is equipped with tiny adsorption columns, which fit tightly and are easy to use. It is suitable for quickly providing large quantities of high-purity micro-samples for nucleic acid or protein sample detection, such as rapid nucleic acid detection. extraction and detection.

In describing the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientation or positional relationship indicated by "vertical", "horizontal", "top, bottom", "inner", "outer" and so on is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as limiting the present invention. In addition, the terms "first", "second", etc. only For descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly referring to the number of technical features indicated. Thus, features defined with "first", "second", etc. may be express or implicit Include one or more of these features. In the description of the present invention, unless otherwise specified, the meaning of "plurality" is two or more.

In the description of the present invention, it should be noted that unless otherwise specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection. Connected, or integrally connected: it can be a mechanical connection, or an electrical connection: it can be directly connected, or indirectly connected through an intermediary, and it can be the internal communication of two components. For those of ordinary skill in the art, The specific meanings of the above terms in the present invention can be understood through specific circumstances.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention. It should be pointed out that for those of ordinary skill in the art, some improvements can be made without departing from the technical principle of the present invention. and modifications, these improvements and modifications should also be considered as the protection scope of the present invention.

Claims (10)

1. The utility model provides a filter core suction head with adsorption column, includes suction head main part (1) of toper structure, the top of suction head main part (1) is provided with feed liquor hole (4), the bottom of suction head main part (1) is provided with inhales hole (5), its characterized in that, one side that is close to feed liquor hole (4) in suction head main part (1) is provided with filter core (2), one side that is close to in suction head main part (1) inhales hole (5) is provided with adsorption column (3).

2. The suction head of a filter element with a suction column as claimed in claim 1, characterized in that the thickness of the filter element (2) is 1-2 mm, and the distance between the top of the filter element (2) and the liquid inlet opening (4) is 3-5 mm.

3. A suction head with a suction column for filter elements according to claim 2, wherein the filter element (2) comprises a first filter layer (6), a second filter layer (7) and a third filter layer (8) from bottom to top in sequence, the thickness ratio of the first filter layer (6), the second filter layer (7) and the third filter layer (8) is 5.

4. A suction head with a filter cartridge and a suction column according to claim 3, characterized in that the first filter layer (6) is made of polyterephthalic acid, the second filter layer (7) comprises 20-30% of polyethylene and 70-80% of carbon-based adsorbent, and the third filter layer (8) is made of polypropylene.

5. A suction head with a suction column according to claim 4, characterized in that the top of the third filter layer (8) is coated with a coating consisting of cellulose acetate.

6. The suction head of a filter element with an adsorption column as claimed in claim 1, characterized in that the thickness of the adsorption column (3) is 4-6 mm, and the distance between the bottom of the adsorption column (3) and the suction hole (5) is 8-10 mm.

7. The suction head of the filter element with the adsorption column as claimed in claim 1, wherein the aperture of the liquid inlet hole (4) is 0.5-1.5 mm, and the aperture of the suction hole (5) is 4-5.5 mm.

8. The suction head with the adsorption column of the filter element as claimed in claim 1, characterized in that the adsorption column (3) is a nucleic acid adsorption column or a protein adsorption column.

9. The method of claim 1, further comprising the steps of:

s1, sucking a sample through a sucking hole (5) at the bottom of a sucking head main body (1), and repeating for multiple times;

s2, sucking a cleaning buffer solution through a suction hole (5) at the bottom of the suction head main body (1), passing through the column, discharging, and repeating for multiple times;

s3, absorbing elution buffer solution through an absorption hole (5) at the bottom of the suction head main body (1) and discharging for later use, and repeating the steps for multiple times;

the elution buffer solution left for use in the step S3 is the sample after extraction and purification.

10. The method of claim 9, wherein the step of sucking the sample in step S1 is repeated three times, the step of sucking the washing buffer solution through the column and discharging the washing buffer solution in step S2 is repeated three times, and the step of sucking the elution buffer solution for discharging the washing buffer solution in step S3 is repeated three times.

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