CN1155081A - Liquid carrying device, and method for mfg. of same - Google Patents
Liquid carrying device, and method for mfg. of same Download PDFInfo
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- CN1155081A CN1155081A CN 96109426 CN96109426A CN1155081A CN 1155081 A CN1155081 A CN 1155081A CN 96109426 CN96109426 CN 96109426 CN 96109426 A CN96109426 A CN 96109426A CN 1155081 A CN1155081 A CN 1155081A
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- 239000007788 liquid Substances 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims description 23
- 239000000463 material Substances 0.000 claims abstract description 59
- 230000000873 masking effect Effects 0.000 claims description 17
- 239000003153 chemical reaction reagent Substances 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 229920000620 organic polymer Polymers 0.000 claims description 3
- 229920001971 elastomer Polymers 0.000 claims description 2
- 239000005060 rubber Substances 0.000 claims description 2
- 238000007493 shaping process Methods 0.000 claims description 2
- 229920005992 thermoplastic resin Polymers 0.000 claims description 2
- 239000011368 organic material Substances 0.000 claims 2
- 239000004634 thermosetting polymer Substances 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 14
- 238000004458 analytical method Methods 0.000 abstract description 7
- 229920002521 macromolecule Polymers 0.000 abstract description 3
- 239000012530 fluid Substances 0.000 description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 18
- 239000004793 Polystyrene Substances 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 4
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 4
- 239000004926 polymethyl methacrylate Substances 0.000 description 4
- 229920002223 polystyrene Polymers 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000004506 ultrasonic cleaning Methods 0.000 description 3
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 2
- -1 Polyethylene Polymers 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- DCKVNWZUADLDEH-UHFFFAOYSA-N sec-butyl acetate Chemical compound CCC(C)OC(C)=O DCKVNWZUADLDEH-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 150000003504 terephthalic acids Chemical class 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 238000009736 wetting Methods 0.000 description 2
- DHKHKXVYLBGOIT-UHFFFAOYSA-N 1,1-Diethoxyethane Chemical compound CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 description 1
- 239000004641 Diallyl-phthalate Substances 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004640 Melamine resin Substances 0.000 description 1
- 229920000877 Melamine resin Polymers 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000004721 Polyphenylene oxide Substances 0.000 description 1
- 239000004734 Polyphenylene sulfide Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 229920001328 Polyvinylidene chloride Polymers 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000011354 acetal resin Substances 0.000 description 1
- 229920000122 acrylonitrile butadiene styrene Polymers 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- QUDWYFHPNIMBFC-UHFFFAOYSA-N bis(prop-2-enyl) benzene-1,2-dicarboxylate Chemical compound C=CCOC(=O)C1=CC=CC=C1C(=O)OCC=C QUDWYFHPNIMBFC-UHFFFAOYSA-N 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000011344 liquid material Substances 0.000 description 1
- 239000002052 molecular layer Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 229920001568 phenolic resin Polymers 0.000 description 1
- 239000005011 phenolic resin Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920006324 polyoxymethylene Polymers 0.000 description 1
- 229920006380 polyphenylene oxide Polymers 0.000 description 1
- 229920000069 polyphenylene sulfide Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920002050 silicone resin Polymers 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229920001169 thermoplastic Polymers 0.000 description 1
- 229920001187 thermosetting polymer Polymers 0.000 description 1
- 239000004416 thermosoftening plastic Substances 0.000 description 1
- 229920006337 unsaturated polyester resin Polymers 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 230000010148 water-pollination Effects 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A simple device for holding liquid for an analysis of a liquid sample, said device comprising a material composed of organic macromolecule, having a surface divided into a plurality of areas which posses contact angles with regard to liquid that are different from each other, one of which is surrounded with the other. With regard to the analysis of test liquids, the device enables the easy holding of even minute quantities of liquid and offers sufficient quantitative precision for analysis.
Description
The present invention relates to a kind of method that is used for the device (being called the carrier fluid device hereinafter) of the loading liquid of analyzing liquid sample and makes this device.Carrier fluid device of the present invention is suitable in measuring the composition of blood, urine and other material the instrument as clinical analysis of diagnosis.
When sample was blood, urine or another kind of liquid, the composition of analyzing a sample needed a container or a loading attachment that is used to load this liquid.The glass apparatus of test tube and other all is the container of common loading liquid.Transfer pipet be used to pack into the sample of fixed amount.For loading attachment, the test specimen of being made up of paper, organic high molecular layer and other the material of fluid permeable is well-known.Under the situation of such loading attachment, suck a certain amount of testing liquid by transfer pipet, allow these liquid pass test specimen and be loaded in the device.
But,, when collecting the testing liquid of trace, can produce some difficulties, and hinder accuracy with pack into the method for testing liquid of test tube or other glass apparatus.The liquid that analysis can be passed test specimen need use transfer pipet, and this has not only increased the complicacy of operation, and has hindered the miniaturization of attempting to make the employed machine of running program robotization.
Therefore, with regard to the analysis of testing liquid, the objective of the invention is to propose a kind of simple carrier fluid device, this device makes and loads on call liquid, even the flowing fluid ratio of the trace of packing into is easier to, and provides enough quantitative precision for analysis.
To achieve the above object, in the carrier fluid device involved in the present invention the material of being made up of organic macromolecule is housed.The surface of this material is made up of different zones, and these zones have mutually different contact angle about liquid.The first area forms less relatively contact angle α for any surface that is attached to the liquid in this zone (first area); Second area near the first area, forms relatively large contact angle β for the surface of the liquid same as described above that is attached to this zone (second area).
Manufacturing makes liquid can be loaded into carrier fluid device wherein for the analytic liquid sample suitable method comprises following main process characteristic:
(a) step of preparation organic macromolecule material.
(b) will be placed in a certain position of material surface to the masking layer (mask) of shaping type, this masking layer and material surface form and closely contact or be separated from each other.
(c) pass the step that masking layer shines material surface with ultraviolet ray.
Liquid in the carrier fluid device, except the fluid sample that needs are analyzed, also comprise dissolving will with the liquid of the reagent of testing sample reaction.
Fig. 1 represents the carrier fluid device of embodiment 1, wherein has water to adhere to from the teeth outwards.Fig. 1 (a) is a vertical view, and Fig. 1 (b) is a front elevation.
Fig. 2 is the sectional view of the carrier fluid device of embodiment 2, wherein has water to adhere to from the teeth outwards.
The surface of the material of the carrier fluid device among the present invention is made up of different zones, and these zones are right Have separately different contact angles in liquid, and these zones mutually near. Because contact angle is not With, if the amount of liquid is seldom, this liquid will be attached to the first area, the first area has littler Contact angle (making liquid be easy to wetting first area), and can not be attached to second area, second There is bigger contact angle (making liquid be difficult to wetting second area) in the zone.
For example, if the area of first area (having littler contact angle) under higher precision Be determined, and on every side around second area (having bigger contact angle), be attached to so The amount of the lip-deep liquid in one zone can be determined under very high precision, the amount of this liquid with The area of first area is relevant with the contact angle that is formed by liquid and first area, and with provide The amount of liquid is irrelevant. This with regard to so that accompanying liquid can advance by the reagent that uses known quantity The row quantitative analysis. Reagent also can be applied to have the zone of littler contact angle in advance.
If employed material is two transparent sheets staggered relatively, and if between two thin slices Distance fixed by the pad that inserts between two thin slices, the first area is as basal plane so, liquid Be loaded into two spaces between the sheeting with cylindrical shape. In this case, only at thin slice The surface of a thin slice of material can provide the zones of different with different contact angles, and reagent can Be applied to the surface of another thin slice. This is so that liquid adheres to front use reagent, and does not change first The contact angle in zone. And, because testing liquid is loaded between two sheetings liquid to be measured The leakage of body is very difficult, and the staff can avoid any infection from testing liquid. This Plant the pollution that arranges also can prevent analytical equipment.
Such as top explanation, the amount that is attached to the liquid on the material surface of the present invention not only depends on The surface area of material also depends on contact angle. In the preparation process of carrier fluid device of the present invention, The contact angle of each isolated area of control material is very important. For example, if the liquid that loads Be water base liquid, arrange preferably to be that the bigger contact angle β of hydrophobic region is at least 60 °, Contact angle difference (β-α) be at least 30 ° at a zone and adjacent area. Such In the parameter area, during repeated experiments, the variation of the amount of accompanying liquid is littler. When the carrier fluid device When preparing with the method for ultraviolet ray irradiation, the ultraviolet irradiation that can below using, will explain Condition is controlled contact angle.
Have the zones of different of different contact angles for the same surface at one piece material produces, (or hydrophilic) of hydrophobic, organically, prepare masking layer on the surface of macromolecular material, like this Hydrophilic group (or group of hydrophobic) and grafted branches are introduced in masking layer by chemical action Exposure portion. Can use the plasma process method, corona discharge process method and other Method replaces chemical method and so that only be that the surperficial position that exposes becomes and has hydrophily and (or hate Water-based), but the method for above-mentioned ultraviolet irradiation is extraordinary, because it is without any need for specially Preliminary treatment or the post processing of door, and used equipment is simple.
In the middle of following one or more can be used as the required organic polymer of material of the present invention: Polyethylene, polypropylene, polystyrene, ABS resin, polyvinyl chloride, polyvinyl dichloride, thermoplastic Based polyurethane, polymethyl methacrylate, PEO, fluororesin, Merlon, polyamides Amine, acetal resin, polyphenylene oxides, poly-terephthalic acids butyl ester, poly-terephthalic acids ethyl ester, polyphenylene sulfide, Or other thermoplastic resin; Unsaturated polyester resin, epoxy resin, phenolic resins, urea tree Fat, melamine resin, poly-diallyl phthalate, silicone resin or other thermosetting The property resin; Silicon rubber or other rubber.
Material can be processed to sheet, cylindric, cylindric, gel, film or fiber shape. The most basically, if can make a panel region have littler contact in order to load the testing liquid material This material so just can be used in the angle. Shape with zone of littler contact angle is generally round Shape, its diameter is between 1mm and 7mm; If polygon, its length on one side is at 1mm And between the 7mm; Or be linear, its width is between 1mm and 5mm. Above-mentioned preparation side Ultraviolet light source in the method is Cooper-Hewitt lamp. This is because tube wall temperature must be lower, approximately 100 ℃, fluorescent tube radiation high-energy, short wavelength's ultraviolet ray. Short wavelength's ultraviolet wavelength is 185nm or 254nm are relatively good, because have higher energy at this length ultraviolet line.
Shine common a period of time of 1 minute to 120 minutes of needs, the distance of irradiation is at 0.5cm to 8.0cm, and exposure intensity is greatly about 1mW/cm
2To 20mW/cm
2
Prepare (each is five) thin slice of three kinds of materials: polymethylmethacrylate (PMMA), poly-terephthalic acids butyl ester (PET) and polycarbonate (PC), each sheet thickness is 0.5mm, prepares two classes and has the masking layer that diameter is the circular light hole of 2mm or 3mm.
Material carries out ultrasonic cleaning in 50% ethanol water, dry then.Then a masking layer is placed on the surface of material, fixes Cooper-Hewitt lamp perpendicular to 2cm place directly over the material.Shine material 10 minutes with ultraviolet ray then, produce first embodiment of carrier fluid device of the present invention.Be right after top process, the carrier fluid device is immersed in the water, take out lightly then, the water of checking material surface is to guarantee that water is only attached to the border circular areas corresponding to the light hole of masking layer, on the surface of material two adjacent areas are just arranged like this, there is different contact angles in these two zones about above-mentioned glassware for drinking water, and separate with circular boundary.Above-mentioned carrier fluid device has adhered to vertical view after the water and front elevation shown in Fig. 1 (a) and Fig. 1 (b) on the surface.Device and water are respectively with symbol among the figure " 1 " and " W " expression.The amount of the water that adheres on this carrier fluid apparatus surface uses the method for KarlFischer to measure.Measurement result is listed in the table 1.
In addition, in order to measure two above-mentioned zone contact angles separately, prepare aforesaid three kinds of materials (each is 10) thin slice by the method for ultrasonic cleaning in 50% ethanol water.Then with the material drying.Next, the water droplet of 1.8 μ l is fallen 5 of each material above the thin slice, and record static contact angle (before ultraviolet irradiation).The remaining 5 line thin slices of each material carry out ultraviolet irradiation under the condition identical with aforesaid carrier fluid device embodiment, then with identical method irradiation area drip on the water used with the thin slice that does not shine.Record static contact angle (after ultraviolet irradiation) then.The results are shown in Table 1 in these measurements.
The amount (μ g) 1927.0 ± 51.1 876.4 ± 30.8 532.2 ± 29.4 of 31 42 23 attached waters is as seen in the table 1 behind preceding 79 77 94 ultraviolet irradiations of table 1 (n=5) material category PMMA PET PC masking layer hole diameter (mm) 322 static contact angles (degree) ultraviolet irradiation, the embodiment of above-mentioned carrier fluid device can load the water of the fixed amount of trace, even be lower than the microlitre magnitude, have higher precision.The delivered payload capability of carrier fluid device not only can be regulated by the surface area size that changes ultraviolet irradiation, and can regulate by the control contact angle.Each material of this embodiment is transparent, like this can be so that the water that adheres to absorb light, and measure the amount of the light that passes material and carry out absorptiometry.Two zones that can not alleged occurrence on the macroscopic view before material immerses in the water have different contact angles.But if before material is carried out ultraviolet irradiation, along the inner periphery line of the light hole of masking layer, the border in two zones can recognize so.
Second embodiment of carrier fluid device of the present invention makes an explanation with reference to diagram.Fig. 2 is the sectional view of second embodiment of carrier fluid device of the present invention.In this example, carrier fluid device ll by two sheetings 12 staggered relatively and 13 and pad 14 form, pad 14 be used to separate and immobilization material 12 and material 13 between distance.
Material 12 and material 13 all are transparent polystyrene (PS) thin slices, and their thickness is lmm, with the contact angle of the water on the surface that is attached to them be 90 °.Only the central authorities on the surface of thin slice 12 and thin slice 13 diameter is arranged is the border circular areas of 3mm (not marking in the drawings), this border circular areas possess hydrophilic property makes that the contact angle of they and water is 15 °.Reagent is used in the border circular areas of material 13.Carrier fluid device 11 is by following step manufacturing.Explore ultraviolet irradiation condition (for example irradiation time) to PS and the relation between the contact angle at first, by experiment.Ethanol water ultrasonic cleaning thin polystyrene sheet with 50%, dry then, then thin polystyrene sheet is positioned in the ultraviolet irradiation equipment.With one have diameter be the masking layer of the circular light hole of 3mm be placed on thin slice above, at Cooper-Hewitt lamp of masking layer upper fixed.Pass masking layer irradiation material with ultraviolet ray then, till the contact angle of border circular areas and water is 15 °.In addition, a kind of reagent solution that contains following component of preparation.
Peroxidase: 4,000 units
Glucose oxidase: 4,000 units
4-amino-antipyrine: 10mg
1-naphthols-3,6-sodium disulfonate: 12mg
0.1M phosphate buffered solution (pH:6): 3ml
Polyvinyl pyrrolidone: 50mg
After ultraviolet irradiation, the drop of the reagent solution of one 1 μ l is dropped in the border circular areas on the thin slice, then with the thin slice drying, obtain material 13 thus.Except not dripping the reagent, obtain material 12 with identical method.Regional staggered relatively in the ultraviolet ray of being exposed to material 12 and material 13 is inserted and secured on pad 14 between the thin slice, and the space between thin slice 12 and the thin slice 13 is 0.3mm.This has just finished the making of the embodiment of second carrier fluid device 11 of the present invention.D/W is circulated into space between material 12 and 13.The result is the border circular areas that D/W (representing with symbol " W " among the figure) only is attached to aforesaid every block of material, and as shown in Figure 2, and the cylindrical shape of solution is loaded.Allow the carrier fluid device keep 1 minute, wavelength is the border circular areas that the light vertical irradiation of 550mm passes material 12 and material 13, and measures absorbance.The measurement result of the absorbance of the D/W of three kinds of variable concentrations is listed in the table 2.
Table 2
The concentration of glucose absorbance
(mg/dl) mean value ± deviation
100 0.102±0.006
200 0.187±0.009
400 0.390±0.017
Seen in table 2, almost directly proportional relation between the concentration of absorbance and D/W.The amount that these results have proved conclusively the D/W that loads between material 12 and the material 13 is certain.
Claims (13)
1. device that is used for the loading liquid that the liquid sample analyzes, this device comprises:
A kind of material of forming by organic polymer, the surface of this material is divided into different zones;
The first area for the surface attached to any liquid in this zone form less relatively contact angle α and
Second area is near above-mentioned first area, and forms relatively large contact angle β for the surface attached to the liquid same as described above in this zone.
2. according to the device of claim 1, wherein use in the first area with the reagent of example reaction.
3. according to the device of claim 1, wherein second area surrounds the first area.
4. according to the device of claim 1, wherein material is transparent.
5. according to the device of claim 1, organic polymer wherein is at least a to be to be selected from the set that thermoplastic resin, thermoset resin, rubber and their composition are formed.
6. according to the device of claim 1, wherein material is sheet, gel, film, column or fibrous.
7. according to the device of claim 1, wherein β is not less than 60 °, and the difference of α and β is not less than 30 °.
8. device that is used for the loading liquid that the liquid sample analyzes, this device comprises:
The transparent sheet of two high-molecular organic materials, these two thin slices are staggered relatively, and the surface of at least one thin slice is divided into different zones,
The first area forms less relatively contact angle α for the surface of any liquid that is attached to this zone,
Second area is near the first area, and second area forms relatively large contact angle β for the surface of the liquid same as described above that is attached to this zone; With
Between two above-mentioned thin slices, insert a pad, be used for fixing two spaces between the thin slice.
9. device according to Claim 8, wherein use in the first area will with the reagent of example reaction.
10. device according to Claim 8, wherein second area surrounds the first area.
11. device according to Claim 8, wherein β is not less than 60 °, and the difference of α and β is not less than 30 °
12. a manufacturing is used for the method for the device of the loading liquid that the liquid sample analyzes, this method may further comprise the steps:
(a) prepare high-molecular organic material.
(b) will be placed on above the material for the masking layer of shaping type, this masking layer forms tight the contact with material surface, or separated.
(c) pass masking layer irradiation material surface with ultraviolet (μ V) line.
13., wherein masking layer is manufactured the part that shielding of ultraviolet passes and surrounds the part that allows ultraviolet ray pass according to the method for claim 12.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96109426 CN1155081A (en) | 1995-08-09 | 1996-08-09 | Liquid carrying device, and method for mfg. of same |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP225699/95 | 1995-08-09 | ||
| JP175657/96 | 1996-06-14 | ||
| CN 96109426 CN1155081A (en) | 1995-08-09 | 1996-08-09 | Liquid carrying device, and method for mfg. of same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1155081A true CN1155081A (en) | 1997-07-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96109426 Pending CN1155081A (en) | 1995-08-09 | 1996-08-09 | Liquid carrying device, and method for mfg. of same |
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| Country | Link |
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| CN (1) | CN1155081A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108780081A (en) * | 2015-08-10 | 2018-11-09 | Essenlix公司 | Simplified steps, small samples, fast and easy-to-use biological/chemical analysis devices and methods |
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1996
- 1996-08-09 CN CN 96109426 patent/CN1155081A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108780081A (en) * | 2015-08-10 | 2018-11-09 | Essenlix公司 | Simplified steps, small samples, fast and easy-to-use biological/chemical analysis devices and methods |
| CN108780081B (en) * | 2015-08-10 | 2021-04-09 | Essenlix公司 | Simplified, small sample, fast, easy-to-use biological/chemical analysis device and method |
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