CN115339135A - Preparation method of silk fibroin colored contact lens - Google Patents

Abstract

The invention discloses a preparation method of silk fibroin colorful contact lenses, which comprises the steps of dissolving and dialyzing a silk fibroin crude product, and then reacting the silk fibroin crude product with acrylamide in a mould to prepare the silk fibroin contact lenses; soaking and dyeing the prepared lens by using the prepared anthocyanin aqueous solution; solves the technical problems that the contact lenses in the prior art have poor air permeability, and the long-term wearing of the contact lenses can cause oxygen deficiency of the cornea of the eyes, so that the eyes have red swelling, pain, dryness and even vision deterioration.

Description

A kind of preparation method of silk fibroin color contact lens

technical field

The invention belongs to the technical field of contact lenses, in particular to a method for preparing silk fibroin colored contact lenses.

Background technique

Contact lenses, also known as contact lenses, are mostly used for vision correction for patients with refractive errors such as myopia and astigmatism, and some of them also have the functions of treating and preventing eye diseases. In addition to colorless transparent contact lenses currently on the market, there are also colored contact lenses that add pigments or dye molecules according to the process. Most of the color contact lenses adopt the "sandwich" technology, and the middle pigment layer is imitated iris pattern, which has decorative and cosmetic effects such as brightening, darkening and enlarging. Colored contact lenses are also widely sought after among young people due to their decorative and cosmetic effects. However, the current color contact lens lenses are still mainly made of polymer hydrogel, silicon hydrogel and other materials, and the color part is based on the use of mostly metal pigments. This makes the materials in colored contact lenses pose a threat to the environment, and at the same time further increases the great health hazards to the eyes. In addition, many contact lenses have poor air permeability, and long-term wear will cause corneal hypoxia, causing redness, pain, dryness and even vision loss in the eyes.

Contents of the invention

The technical problem to be solved by the present invention is to provide a preparation method of silk fibroin colored contact lenses to solve the problem that the contact lenses in the prior art have poor air permeability, and long-term wearing will cause corneal hypoxia, causing redness, pain, dryness and even dryness in the eyes. Technical problems such as vision loss.

Technical scheme of the present invention is:

A method for preparing silk fibroin colored contact lenses, the method is that the crude silk fibroin product is dissolved and dialyzed and then reacted with acrylamide in a mold to prepare silk fibroin contact lenses; Aqueous solution for soaking and dyeing.

The acrylamide solution and the silk fibroin solution are evenly mixed and then dropped into a contact lens mold and air-dried to obtain a lens.

The concentration of silk fibroin solution is 5%~15% (g/mL), the concentration of acrylamide solution is 25%~75% (g/mL); the volume ratio of acrylamide solution and silk fibroin solution is 1:10 Mix well.

The method of dissolution and dialysis of the crude silk fibroin product is as follows: the crude silk fibroin product is completely submerged in sterile ultrapure water, dissolved by ultrasonic cleaner, and the solution is dialyzed in sterile ultrapure water for 3~5 days, every 6~ Sterile ultrapure water was replaced 24 hours a day.

The contact lens mold is washed and dried with sterile water, and then a mixed solution of acrylamide and silk fibroin is added; the temperature of the air-drying process is ^{10-40 o} C.

The air-dried lens is dyed with anthocyanin by the overall soaking method. The overall dipping method includes: soaking the air-dried lens in sterile ultrapure water for 60-120 minutes; Soak in 30% (g/mL) anthocyanin aqueous solution at 4-15 ^o C for 1-2 days; take out the colored lens and soak it in sterile ultra-pure water for 20-200 minutes; use sterile ultra-pure The lens is washed with water to obtain an overall tinted lens.

Silk fibroin color contact lenses include silk fibroin lenses and anthocyanins bound to the silk fibroin lenses; silk fibroin lenses are porous silk fibroin films double-induced by acrylamide molecules, and the preparation method is as follows: acrylamide molecules and The silk fibroin solution is blended to induce the amorphous silk fibroin to form a crystallized and stable porous silk fibroin film, and then the acrylamide is removed by repeated soaking and rinsing; the lens is dyed by the anthocyanin molecular soaking method to obtain the dyed Stabilized colored contact lenses.

Beneficial effects of the present invention:

The main material components and effects thereof of the silk fibroin colored contact lenses provided by the invention are as follows:

Silk fibroin: an excellent natural polymer fibrin has been widely used in the field of biomedical materials. As a natural biomaterial, silk fibroin has many remarkable properties, such as: good biocompatibility, suitable mechanical properties, low inflammatory response, good gas permeability and controllable degradation rate. At present, silk protein scaffolds have been widely used in various tissue engineering, including the repair of cartilage, nerves, blood vessels, etc. In addition, its reliable source and easy access are also a major advantage of its wide application.

Acrylamide: an organic compound, often used as a raw material for organic synthesis and polymer materials. It is usually soluble in water, and has a good effect on the flocculation of protein and starch in water. It has excellent properties such as flocculation, thickening, shear resistance, drag reduction, and dispersibility.

Anthocyanin: a kind of water-soluble natural pigment that widely exists in plants in nature. It has strong anti-oxidation, anti-mutation, and anti-allergic abilities. It can enhance blood vessel elasticity, improve circulatory system, improve sleep, and inhibit inflammation and allergies. At the same time, it can also prevent vision loss and protect the eyes. And it has a certain inhibitory effect on elastase and collagenase, improves the smoothness of the skin, delays aging, and prevents skin damage symptoms caused by sun exposure. It is sensitive to the environment, pH value, co-chromatic effect, etc. will affect its coloration.

In summary, the silk fibroin colored contact lens provided by the present invention has at least the following beneficial effects:

High safety : The silk fibroin and anthocyanins used in the preparation of lenses are all derived from nature, have good biocompatibility, and are safe to the human body. The porous structure of the lens makes it highly oxygen permeable, which can avoid eye redness and pain caused by corneal hypoxia, and protect the wearer's eye health.

Multifunctional : the silk fibroin color contact lens of the present invention can play the role of anthocyanin active ingredient and prevent vision loss. Based on the active ingredients of anthocyanin molecules, the detection of eye diseases can also be realized.

Environmental protection : the silk fibroin and anthocyanins that make up the lens can be degraded in the natural environment, and have no pollution to the environment.

The technical problems of poor air permeability of the contact lenses in the prior art, long-term wearing will cause hypoxia of the cornea, redness, swelling, pain, dryness and even vision loss in the eyes are solved.

Description of drawings

Fig. 1 is the preparation flowchart of the silk fibroin color contact lens in one embodiment of the present invention;

Fig. 2 is a schematic longitudinal sectional view of an integrally colored silk fibroin colored contact lens in an embodiment of the present invention.

Detailed ways

The invention adopts the following technical scheme: after the crude silk fibroin product is dissolved and dialyzed, it is reacted with acrylamide in a mold to prepare silk fibroin contact lenses; after the lens is prepared, it is soaked and dyed by the configured anthocyanin aqueous solution.

As shown in Figure 1, the preparation process includes the following main steps: mixing acrylamide reagent and silk fibroin solution; dropping the mixed solution into a contact lens mold, air-drying and setting; dyeing.

The preparation method of the lens comprises: mixing the acrylamide solution and the silk fibroin solution, dropping them into a contact lens mold, and air-drying to obtain the lens.

The concentration of the silk fibroin solution is 5% to 15% (g/mL), the concentration of the acrylamide solution is 25% to 75% (g/mL); the acrylamide solution and the silk The protein solution was mixed according to the volume ratio of 1:10.

The crude silk fibroin protein product is completely submerged in sterile ultrapure water, and ultrasonically dissolved by an ultrasonic cleaner; the solution is dialyzed in sterile ultrapure water for 3 to 5 days, and the sterile ultrapure product is replaced every 6 to 24 hours. water.

The contact lens mold is washed and dried with sterile water, and then a mixed solution of acrylamide and silk fibroin is added; the temperature of the air-drying process is 10-40 ^° C.

Carry out anthocyanin dyeing to the air-dried lenses by adopting the overall soaking method;

The overall dip-dyeing method includes the following steps: soak the air-dried lens in sterile ultrapure water for 60-120 minutes; then completely immerse the lens in a 5-30% (g/mL) anthocyanin aqueous solution with a pH of 7-8 immersion at a temperature of 4-15 ^o C for 1-2 days; take out the colored lens and soak it in sterile ultrapure water for 20-200 minutes; wash the lens with sterile ultrapure water to obtain an overall colored lens.

The silk fibroin colored contact lens provided by the invention comprises a silk fibroin lens and anthocyanin combined on the silk fibroin lens. The silk fibroin lens is a porous silk fibroin film double-induced by acrylamide molecules, and its preparation method is: blending acrylamide molecules with a silk fibroin solution to induce the amorphous silk fibroin to form crystal-stable porous silk The protein film was then removed by repeated soaking and rinsing to remove acrylamide. In order to successfully color the lenses with anthocyanin molecules, the present invention adopts an anthocyanin molecule soaking method to dye the lenses. In this method, the anthocyanin molecules and the amino acid residues in silk fibroin have electrostatic adsorption physical effects, thereby obtaining stable dyeing. colored contact lenses. The dyeing principle can improve the binding stability of anthocyanin molecules and silk fibroin on the one hand, and retain the physical and chemical properties of anthocyanin molecules on the other hand.

In the film-forming reaction of silk fibroin induced by acrylamide molecules, the concentration of silk fibroin is 5%-15%, and the concentration of acrylamide is 2.5%-7.5%. In some embodiments, the temperature range at which the acrylamide molecule induces silk fibroin film formation is 10-40 ^° C. In some embodiments, the humidity range for the acrylamide molecule to induce silk fibroin film formation is 10%-50%. In some embodiments, the anthocyanin-dyed tinted lenses are integrally tinted tinted lenses. In some embodiments, the dyeing method of the overall colored color chip is soaking method.

The technical solution of the present invention will be clearly and completely described below in conjunction with the examples. It should be understood that the following examples are only for explaining the present invention, and are not intended to limit the scope of the present invention.

Main reagents :

Acrylamide was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., item number A108465-100g. Silk fibroin was purchased from Suzhou Silkmate Biotechnology Co., Ltd., product number LPSXKOA001. Anthocyanins were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., item number C302404-25g.

Solution preparation :

2.5% (g/mL) acrylamide solution: Weigh 0.25g of acrylamide solid particles into 10mL ultrapure water to prepare a 2.5% (g/mL) acrylamide solution.

5% (g/mL) silk fibroin solution: Weigh 2.5g of silk fibroin solid particles and add it into 50mL ultrapure water to prepare a 5% (g/mL) silk fibroin solution.

5% (g/mL) anthocyanin aqueous solution (pH=7.5): Weigh 0.5g of anthocyanin solid particles and add it to 10mL sterile ultrapure aqueous solution to prepare a 10% (g/mL) anthocyanin aqueous solution .

Unless otherwise specified, the reagents used in the following examples are all conventional reagents in this field, which are commercially available or prepared according to conventional methods in this field, and the specifications are laboratory pure grade. Unless otherwise specified, the experimental methods and conditions used in the following examples are conventional experimental methods and conditions in the art, and reference can be made to relevant experimental manuals, known documents or manufacturer's instructions. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

Embodiment 1, the preparation of the overall coloring type porous silk fibroin contact lens of anthocyanin dyeing

1. Preparation of porous silk fibroin lenses

Take 0.1 mL of 25% (g/mL) acrylamide solution and 1 mL of 5% (g/mL) silk fibroin solution, add them into a 5mL centrifuge tube and mix evenly, take 100 μL of the mixture dropwise into the invisible Place the glasses mold in a ventilated place at 10-40°C to air-dry, and soak the silk fibroin lenses in sterile ultra-pure water for 60 minutes, then remove the soaking liquid, add sterile ultra-pure water to wash for 30 minutes, and repeat this process for three times. Finally, put the cleaned silk fibroin lens into sterile physiological saline and save it for later use.

2. Anthocyanin dyeing

Put the silk fibroin lens prepared and cleaned in step 1 into a centrifuge tube containing 5% (g/mL) anthocyanin aqueous solution (pH=7.5), put it in a 4 ^o C refrigerator, and take it out after 1 day Soak the silk fibroin lenses dyed with anthocyanin in sterile ultrapure water for 30 minutes, remove the soaking solution and add sterile ultrapure water to wash, repeat this process for three times, and finally wash the anthocyanin-dyed lens The lenses are stored in sterile saline for later use.

Embodiment 2, the preparation of the annular coloring type silk fibroin contact lens of anthocyanin dyeing

1. Preparation of porous silk fibroin lenses

Take 0.1 mL of 50% (g/mL) acrylamide solution and 1 mL of 15% (g/mL) silk fibroin solution, add them into a 5 mL centrifuge tube and mix evenly, take 100 μL of the mixed solution dropwise into Place the contact lens mold in a ventilated place at 10-40°C to air-dry, and soak the silk fibroin lens in sterile ultra-pure water for 80 minutes, then remove the soaking solution, add sterile ultra-pure water to wash for 30 minutes, and repeat this process for three times , and finally put the cleaned silk fibroin lens into sterile saline for storage until use.

2. Anthocyanin dyeing

Put the silk fibroin lens prepared and cleaned in step 1 into a centrifuge tube containing 10% (g/mL) anthocyanin aqueous solution (pH=7.5), put it in a 4 ^o C refrigerator, and take it out after 1 day Soak the silk fibroin lenses dyed with anthocyanin in sterile ultrapure water for 30 minutes, remove the soaking solution and add sterile ultrapure water to wash, repeat this process for three times, and finally wash the anthocyanin-dyed lens The lenses are stored in sterile saline for later use.

Claims (7)

1. A method for preparing silk fibroin colored contact lenses, characterized in that: the method is to dissolve and dialyze the crude silk fibroin product and react with acrylamide in a mold to obtain silk fibroin contact lenses; Dyeing by immersion in the prepared anthocyanin aqueous solution. 2. A method for preparing silk fibroin colored contact lenses according to claim 1, characterized in that: after mixing the acrylamide solution and the silk fibroin solution, they are dropped into contact lens molds and air-dried to obtain lenses. 3. A method for preparing silk fibroin colored contact lenses according to claim 2, characterized in that: the concentration of the silk fibroin solution is 5%-15% (g/mL), and the concentration of the acrylamide solution is 25% ~75% (g/mL); acrylamide solution and silk fibroin solution were mixed at a volume ratio of 1:10. 4. The preparation method of a kind of silk fibroin color contact lens according to claim 1, characterized in that: the method of dissolving and dialysis of the crude silk fibroin product is: putting the crude silk fibroin product into sterile ultrapure water and submerging it completely, Ultrasonic dissolution by an ultrasonic cleaner, the solution is dialyzed in sterile ultrapure water for 3 to 5 days, and the sterile ultrapure water is replaced every 6 to 24 hours. 5. a kind of silk fibroin colored contact lens preparation method according to claim 1 is characterized in that: contact lens mold is added acrylamide and silk fibroin mixed solution after cleaning and drying with aseptic water; Described air-drying process temperature 10~ ^40oC . 6. A kind of silk fibroin color contact lens preparation method according to claim 1, is characterized in that: adopt integral immersion method to carry out anthocyanin dyeing to air-dried eyeglass, integral dip-dyeing method comprises: soak air-dried eyeglass in Bacterial ultrapure water for 60-120 minutes; then completely immerse the lens in a 5-30% (g/mL) anthocyanin aqueous solution with a pH of 7-8 at a temperature of 4-15 ^o C for 1-2 days; Take out the colored lens, soak it in sterile ultrapure water for 20-200 minutes; wash the lens with sterile ultrapure water, and obtain the overall colored lens. 7. The preparation method of a kind of silk fibroin colored contact lens according to claim 1, characterized in that: the silk fibroin colored contact lens comprises silk fibroin lens and anthocyanin combined on the silk fibroin lens; The protein lens is a porous silk fibroin film induced by acrylamide molecules. The preparation method is: blending acrylamide molecules with a silk fibroin solution to induce the amorphous silk fibroin to form a crystalline stable porous silk fibroin film, and then The acrylamide is removed by repeated soaking and rinsing; the lens is dyed by an anthocyanin molecule soaking method, so as to obtain a color contact lens with stable dyeing.

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