CN114886817B - French willow leaf and saccharomycete zinc fermentation compound, preparation method and application - Google Patents

Abstract

The invention discloses a fermented compound of French willow leaf and yeast zinc, a preparation method and application. The preparation method comprises the following steps: drying and crushing the French willow leaf; Put the willow leaf powder into a pressurized extraction device, add water and polyhydric alcohol, extract at a temperature of 90-140°C and a pressure of 0.5-1.0 MPa, and filter to obtain an extract containing evening primrose B; add to the extract Yeast and zinc compound are added to the mixture, fermented and filtered to obtain the French willow leaf and yeast zinc fermentation compound, and the prepared French willow leaf and yeast zinc fermentation compound can be used as cosmetic raw materials. The invention has the advantages of improving the yield of evening primrose B in the extract of the Fleischer willow leaf, and the fermentation compound of the Fletcher's willow leaf and yeast zinc has good oil control effect and the like.

Description

A fermented compound of French willow leaf and yeast zinc, preparation method and application

technical field

The invention belongs to the technical field of plant extraction, and in particular relates to a fermented complex of French willow leaf and yeast zinc, a preparation method and application.

Background technique

Fleischer's willow is a herbaceous perennial that grows in open habitats. The stem is cylindrical, green, turns light red in autumn, and is pilose. Widely distributed in tropical and temperate regions, but mostly in western North America; most abundant in southwest China. There is a certain amount of evening primrose B in Fleischer's willow leaf. Evening primrose B is a macrocyclic dimer ellagitannin, which is commonly used in the medical field, such as anti-tumor, anti-inflammatory, immune regulation, anti-oxidation and anti-fungal. Evening primrose B can keep the skin matte and non-greasy for a long time, restore the healthy appearance of the skin, shrink pores, and make the skin more clean and delicate. The existing evening primrose B in French willow leaf is generally obtained by water extraction, which has the problem of low yield of evening primrose B.

Human skin is the tissue with the third highest zinc content in the body at about 5%. Most enzymes in human skin require zinc for maximum catalytic activity, and insufficient zinc reduces growth in almost all biological systems by inhibiting cell replication.

Cosmetics with skin oil control effect on the market generally add evening primrose B, yeast/zinc fermentation product, yeast/zinc fermentation product is fermented by yeast and zinc compound. In the process of cosmetic preparation, evening primrose B and yeast/zinc fermentation product are simply mixed together, and there is only a synergistic effect between the two molecules, which makes the overall skin oil control effect relatively poor.

Contents of the invention

The purpose of the present invention is to solve the problems in the background technology, to propose a fermented complex of Fleische's willow leaf and yeast zinc, preparation method and application, which can improve the evening primrose in the Fletcher's willow leaf extract. The yield of vitamin B, Fleischer's willow leaf and yeast zinc fermentation complex have good oil control effect, and can be used as cosmetic raw materials.

In order to achieve the above object, the present invention proposes a preparation method of French willow leaf vegetable and yeast zinc fermentation complex, comprising the following steps:

Dried French willow leaves and crushed;

Put the French willow leaf powder into a pressurized extraction device, add water and polyhydric alcohol, extract at a temperature of 90-140°C and a pressure of 0.5-1.0 MPa, and filter to obtain an extract containing evening primrose B;

Yeast and zinc compound are added to the extract, fermented and filtered to obtain a fermented complex of French willow leaf and yeast zinc.

Preferably, the mass ratio of the French willow leaf powder to water is 1:100-200, and the mass ratio of water to polyol is 1-2:5.

Preferably, the extraction time is 0.5-4 hours.

Preferably, the fermentation is carried out at a temperature of 10-40° C., and the fermentation time is 10-50 hours.

Preferably, the polyhydric alcohol is 1,2-butanediol, 1,2-hexanediol, 1,2-pentanediol, 1,3-propanediol, 1,5-pentanediol, butanediol, At least one of propylene glycol, hexanediol, 1,6-hexanediol, and 2,3-butanediol.

Preferably, the yeast is at least one of Saccharomyces cerevisiae, Saccharomyces cerevisiae, Schizosaccharomyces saccharomyces, Saccharomyces rouxii, Saccharomyces conjunctica, and Saccharomyces hansenula, and the mass ratio of the yeast to the extract is 1:99-200.

Preferably, the zinc compound is at least one of zinc amino acid, zinc sulfate, zinc chloride, zinc nitrate, zinc citrate, zinc gluconate, zinc stearate, zinc laurate, zinc myristate, zinc The mass ratio of the compound and the extract is 1:80-99.

Preferably, the drying of the French willow leaf vegetable is carried out at a temperature of 60-100°C.

The present invention also proposes a fermented complex of French willow leaf and yeast zinc prepared according to the above preparation method.

The present invention also proposes the application of the above-mentioned French willow leaf vegetable and saccharomyces zinc fermented complex as cosmetic raw materials.

Beneficial effects of the present invention: the present invention extracts the French willow leaf vegetable by adopting a pressurized extraction process, effectively improving the yield of evening primrose B in the extract; The extract of French willow leaf, yeast and zinc compound are mixed, and after biological fermentation reaction, the zinc fermentation compound of French willow leaf and yeast is obtained, and the obtained French willow leaf and yeast The zinc fermented complex has a good skin oil control effect, synergistically reduces sebum secretion, and can be used as a cosmetic raw material.

The features and advantages of the present invention will be described in detail with reference to the accompanying drawings.

Description of drawings

Fig. 1 is the process flow diagram of the embodiment of the present invention.

Fig. 2 is a schematic diagram of the structural formula of the French willow leaf vegetable and yeast zinc fermentation complex according to the embodiment of the present invention.

Fig. 3 is a schematic diagram of the comparison of the yield of evening primrose B under different processes in the embodiment of the present invention.

Fig. 4 is a schematic diagram of comparison of sebum content in the examples of the present invention.

Detailed ways

Referring to Fig. 1 and Fig. 2, the present invention provides a kind of preparation method of Freiche's willow leaf vegetable and yeast zinc fermentation complex, comprises the following steps:

S01. drying and pulverizing the French willow leaf;

S02. Put the French willow leaf powder into a pressurized extraction device, add water and polyols, extract at a temperature of 90-140°C and a pressure of 0.5-1.0 MPa, and filter to obtain the extract containing evening primrose B liquid;

S03. adding yeast and zinc compound to the extract, fermenting, and filtering to obtain a fermented complex of French willow leaf and yeast zinc.

The mass ratio of French willow leaf powder and water is 1:100-200, and the mass ratio of water and polyol is 1-2:5. The French willow leaf powder is fully soluble in water for extraction See Herbalin B.

The extraction time is 0.5-4 hours to optimize the extraction effect.

Fermentation is carried out at a temperature of 10-40°C, and the fermentation time is 10-50 hours. At this temperature, the fermentation reaction between the yeast and the zinc compound is the best, and the fermentation time is sufficient to ensure that the fermentation reaction is complete and sufficient Fleischer Willow Leaf and Saccharomyces Zinc Ferment Complex.

Polyols are 1,2-butanediol, 1,2-hexanediol, 1,2-pentanediol, 1,3-propanediol, 1,5-pentanediol, butanediol, propylene glycol, hexanediol , 1,6-hexanediol, 2,3-butanediol at least one.

The yeast is at least one of brewer's yeast, grape juice yeast, fission yeast, Luxie yeast, combined yeast and Hansenula yeast, and the mass ratio of the yeast to the extract is 1:99-200.

The zinc compound is at least one of zinc amino acid, zinc sulfate, zinc chloride, zinc nitrate, zinc citrate, zinc gluconate, zinc stearate, zinc laurate, zinc myristate, and the zinc compound and the extract The mass ratio is 1:80～99

The zinc compound is at least one of zinc amino acid, zinc sulfate, zinc chloride, zinc nitrate, zinc citrate, zinc gluconate, zinc stearate, zinc laurate, zinc myristate, and the zinc compound and the extract The mass ratio is 1:80-99.

The drying of French willow leaf is carried out at a temperature of 60-100° C., and at least one part of the French willow leaf, its flowers, leaves and stems is preferably dried.

The present invention also proposes a fermented complex of French willow leaf and yeast zinc prepared according to the above preparation method.

The present invention also proposes the application of the above-mentioned French willow leaf and yeast zinc fermented complex as a raw material for cosmetics. Cosmetics include but not limited to skin care water and essence. Zinc fermented complex as a raw material of cosmetics can improve the skin oil control effect of cosmetics.

The present invention is further described below by specific embodiment, and following embodiment is only for illustrating the present invention and does not limit the present invention:

Example 1

The present embodiment provides a kind of preparation method of Fleischer's willow leaf vegetable and yeast zinc fermented complex, comprising the following steps:

S01. Drying the flowers, leaves and stems of Fleischer's willow leaf at a temperature of 60°C, and pulverizing them;

S02. Put the French willow leaf powder into the pressurized extraction device, add water and 1,2-butanediol in proportion, set the temperature and pressure values of the pressurized extraction device to 90°C and 1.0MPa respectively, and Under this temperature and pressure condition, extract 0.5h, filter to obtain the extract containing evening primrose B, wherein, the mass ratio of French willow leaf powder, water and 1,2-butanediol is 1:100: 500;

S03. Add brewer's yeast and amino acid zinc to the extract, ferment at 10°C for 50 hours, and filter to obtain the fermentation complex of French willow leaf and saccharomyces zinc, wherein the quality of the extract, brewer's yeast and amino acid zinc The ratio is 200:1:2.5.

Example 2

The present embodiment provides a kind of preparation method of Fleischer's willow leaf vegetable and yeast zinc fermented complex, comprising the following steps:

S01. Drying the flowers, leaves and stems of Fleischer's willow leaf at a temperature of 100°C, and pulverizing them;

S02. Put the French willow leaf powder into the pressurized extraction device, add water and 1,2-hexanediol in proportion, set the temperature and pressure values of the pressurized extraction device to be 140°C and 0.5MPa respectively, in Extract under the temperature and pressure conditions for 4 hours, filter to obtain the extract containing evening primrose B, wherein the mass ratio of French willow leaf powder, water and 1,2-hexanediol is 1:200:500 ;

S03. Add grape juice yeast and zinc sulfate to the extract, ferment at 40°C for 10 hours, and filter to obtain the Zinc Fermentation Complex of French willow leaf and yeast, wherein the extract, grape juice yeast and zinc sulfate The mass ratio is 99:1:1.

Example 3

The present embodiment provides a kind of preparation method of Fleischer's willow leaf vegetable and yeast zinc fermented complex, comprising the following steps:

S01. Drying the flowers, leaves and stems of Fleischer's willow leaf at a temperature of 80°C, and pulverizing them;

S02. Put the French willow leaf powder into the pressurized extraction device, add water and 1,2-pentanediol in proportion, set the temperature and pressure values of the pressurized extraction device to be 115°C and 0.7MPa respectively, in Under this temperature and pressure condition, extract 2.5h, filter to obtain the extract containing evening primrose B, wherein, the mass ratio of French willow leaf powder, water and 1,2-pentanediol is 1:150: 500;

S03. Add Saccharomyces rouxii and zinc chloride to the extract, ferment at 25°C for 30h, and filter to obtain Fermentation Complex of Fleischer's willow leaf and Saccharomyces zinc, wherein the extract, Saccharomyces rouxii and chlorine The mass ratio of zinc chloride is 150:1:1.6.

Comparative example

This comparative example is a traditional preparation process for preparing a mixture containing French willow leaf and saccharomyces zinc fermentation complex, which specifically includes the following steps:

S01. Drying the flowers, leaves and stems of Fleischer's willow leaf at a temperature of 60°C, and pulverizing them;

S02. Add water and 1,2-butanediol to the French willow leaf powder, the mass ratio of the French willow leaf powder, water and 1,2-butanediol is 1:100:500, Carry out normal pressure extraction, filter to obtain the extract containing evening primrose B;

S03. Mix beer yeast and amino acid zinc, ferment at 10°C for 50 hours, filter to obtain the yeast/zinc fermentation product, and then mix the yeast zinc fermentation complex with the extract to obtain evening primrose B and yeast/zinc A mixture of zinc fermentation products, wherein the mass ratio of the extract, brewer's yeast and amino acid zinc is 200:1:2.5.

Experiment on the influence of pressure extraction on the yield of evening primrose B;

Get some Fleischer's willow leaf cauliflower flowers, leaves and stems that are complete and of the same product appearance quality, and divide them into two groups and mark them as the experimental group and the control group respectively. The evening primrose B in the French willow leaf was extracted, and the control group was extracted the evening primrose B in the French willow leaf according to the first two steps of the preparation method of the control group. The yield of evening primrose B in the extract was measured and calculated, and the specific yield detection results are shown in FIG. 3 .

As can be seen from Figure 3, the yield of evening primrose B using the pressure extraction process is significantly higher than the yield of evening primrose B using the traditional normal pressure extraction process, indicating that the pressure extraction device is used to extract Extraction under pressure is beneficial to improve the extraction effect of evening primrose B in Fleischer willow leaf.

Experiments on the effect of French willow leaf vegetable and yeast zinc fermented complex on skin oil control;

Select 200 test subjects with no obvious skin function defects (no acne) and high forehead sebum content (forehead sebum content > 120ug/cm ² ), and divide them into four groups, which are respectively recorded as the experimental group, the control group 1, and the control group. Group 2 and blank group, experimental group, control group 1, and control group 2 testers applied the same amount of product B, placebo, and product A on the forehead respectively, and blank group experimenters did not do any treatment for 28 days. After 28 days of measurement, each The sebum content of group experimenter, calculate average value, the sebum content detection result of each group experimenter is as shown in Figure 4, wherein, placebo is water, and product B is 3% Fleischer's willow leaf and yeast zinc The aqueous solution of the fermentation complex (the fermentation complex is obtained according to the preparation method of Example 1), the product A is the aqueous solution of the mixture of 3% evening primrose B and yeast/zinc fermentation product (the mixture is obtained according to the preparation method of the comparative example ).

It can be seen from Figure 4 that the sebum content of the experimental group, the control group 2, the control group 1 and the blank group testers increased successively, indicating that the Fleischer Oil-controlling effect of willow leaf and yeast zinc ferment complex on skin compared to evening primrose B and yeast/zinc ferment simply mixed with evening primrose B and yeast/zinc ferment The mixture has a better oil control effect on the skin, and can work better synergistically to reduce sebum secretion.

The above-mentioned embodiment is an illustration of the present invention, not a limitation of the present invention, and any solution after a simple transformation of the present invention belongs to the protection scope of the present invention.

Claims (4)

1. a preparation method of French willow leaf vegetable and saccharomyces zinc fermentation compound, is characterized in that: comprise the following steps: Dry the French willow leaf and crush it; putting the French willow leaf powder into a pressurized extraction device, adding water and polyhydric alcohol, extracting at a temperature of 90-140°C and a pressure of 0.5-1.0 MPa, and filtering to obtain an extract containing evening primrose B; adding yeast and zinc compound to the extract, fermenting, and filtering to obtain the fermented complex of French willow leaf and yeast zinc; Wherein, the mass ratio of the French willow leaf powder and water is 1:100 to 200, the mass ratio of water and polyol is 1 to 2:5, and the extraction time is 0.5 to 4 hours. The fermentation is carried out at a temperature of 10-40°C, and the fermentation time is 10-50h. The polyol is at least one of butanediol, propylene glycol, hexylene glycol, 1,2-pentanediol and 1,5-pentanediol One, the yeast is at least one of Saccharomyces cerevisiae, Grape juice yeast, Schizosaccharomyces saccharomyces, Saccharomyces rouxii, Conjugative yeast, and Hansenula yeast, and the mass ratio of the yeast to the extract is 1:99-200, The zinc compound is at least one of zinc amino acid, zinc sulfate, zinc chloride, zinc nitrate, zinc citrate, zinc gluconate, zinc stearate, zinc laurate, zinc myristate, zinc compound and extract The mass ratio of liquid to liquid is 1:80-99, and the drying of the French willow leaf is carried out at a temperature of 60-100°C. 2. the preparation method of Fleischer's willow leaf vegetable and saccharomyces zinc fermentation compound as claimed in claim 1, is characterized in that: described butanediol is 1,2-butanediol or 2,3-butane Diol, the propylene glycol is 1,3-propanediol, and the hexanediol is 1,2-hexanediol or 1,6-hexanediol. 3. a French willow leaf vegetable and saccharomyces zinc ferment compound that a kind of preparation method according to claim 1 or 2 makes. 4. a kind of Fleischer's willow leaf vegetable as claimed in claim 3 and the application of saccharomycete zinc fermentation compound as cosmetic raw material.

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