CN114813298A - Sample blending simulation test method and device, sample blending device, and storage medium - Google Patents

Abstract

The application provides a sample blending simulation test method and device, sample blending equipment and a storage medium, and relates to the technical field of biological sample detection. According to the method, the first absorbance of a simulation sample matched with the viscosity of a sample to be detected at the maximum absorption wavelength of a fluorescent material is obtained, the second absorbance of a simulation reagent matched with the viscosity of a preset reagent at the maximum absorption wavelength of the fluorescent material is obtained, the third absorbance and the fourth absorbance respectively correspond to a target viscous solvent shared by the simulation reagent and the simulation sample at the two maximum absorption wavelengths, the fifth absorbance and the sixth absorbance respectively correspond to a mixed reaction liquid obtained by uniformly mixing the simulation sample and the simulation reagent with the sample reagent at the two maximum absorption wavelengths, and then the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance and the sixth absorbance are subjected to data analysis to obtain corresponding uniform mixing effect evaluation parameters, so that the meaningless loss of the sample and the reagent to be detected is avoided, and the effect of the specific sample is effectively and quantitatively evaluated and uniformly mixed.

Description

Sample mixing simulation test method and device, sample mixing equipment and storage medium

technical field

The present application relates to the technical field of biological sample detection, and in particular, to a sample mixing simulation test method and device, a sample mixing device and a storage medium.

Background technique

In vitro diagnostic technology is an important branch of modern medical technology, which can detect human samples (such as blood, body fluids, tissues, etc.) outside the human body to obtain clinical diagnostic information to judge diseases or body functions. In the process of detecting human samples, it is often necessary to complete the mixing of human samples and in vitro detection reagents, and to detect the photoelectric signal of the mixed liquid after applying a certain degree of reaction conditions, so as to obtain the analyte in the human sample. The specific information of which the poor mixing of reagents and samples will affect the reaction process of samples and reagents, resulting in abnormal final test results.

With the rapid development of automation technology, the rapid mixing of sample reagents in batches through automated detection equipment can effectively improve the efficiency of sample detection, and has become one of the important directions for the development of in vitro diagnostic technology. Therefore, how to accurately know the mixing effect of sample reagents, and then verify and optimize the sample mixing performance of automated testing equipment, is of great significance to ensure accurate and reliable sample testing results.

SUMMARY OF THE INVENTION

In view of this, the purpose of the present application is to provide a sample mixing simulation test method and device, sample mixing equipment and storage medium, which can effectively treat the sample to be tested and the corresponding reagent while avoiding the meaningless loss of the sample. The specific mixing effect of the mixing operation of the test sample and the corresponding reagent is quantitatively evaluated, which is convenient for the testing personnel to adjust the mixing operation details for the sample to be tested and the corresponding reagent, so that the final test result is accurate and reliable.

In order to achieve the above purpose, the technical solutions adopted in the embodiments of the present application are as follows:

In a first aspect, the application provides a sample mixing simulation test method, the method includes:

acquiring the first absorbance at the first maximum absorption wavelength corresponding to the first fluorescent dye for a simulated sample whose viscosity matches the sample to be tested, wherein the simulated sample is obtained by dissolving the first fluorescent dye into a target viscous solvent;

acquiring a second absorbance at a second maximum absorption wavelength corresponding to the second fluorescent dye for a simulated reagent that matches the viscosity of the preset reagent, wherein the simulated reagent is obtained by dissolving the second fluorescent dye into the target viscous solvent;

obtaining the third absorbance and the fourth absorbance corresponding to the target viscous solvent at the first maximum absorption wavelength and the second maximum absorption wavelength, respectively;

acquiring the fifth absorbance and the sixth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength of the mixed reaction solution obtained by performing the sample reagent mixing operation on the simulated sample and the simulated reagent respectively;

Perform data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance, and the sixth absorbance, and obtain a match with the sample reagent mixing operation The mixing effect evaluation parameters.

In an optional embodiment, performing data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance, and the sixth absorbance, The steps of obtaining the mixing effect evaluation parameters matching the mixing operation of the sample reagents include:

According to the first absorbance, the fifth absorbance and the third absorbance, calculate the first net absorbance coefficient of the mixed reaction solution relative to the simulated sample under the sample reagent mixing operation;

According to the second absorbance, the sixth absorbance and the fourth absorbance, calculate the second net absorbance coefficient of the mixed reaction solution relative to the simulated reagent under the sample reagent mixing operation;

A ratio calculation is performed on the first net light absorption action coefficient and the second net light absorption action coefficient to obtain the mixing effect evaluation parameter matching the mixing operation of the sample reagent.

In an optional embodiment, according to the first absorbance, the fifth absorbance and the third absorbance, calculate the relative value of the mixed reaction solution to the simulated sample under the sample reagent mixing operation The steps of the first net absorptivity coefficient include:

Subtracting the fifth absorbance and the third absorbance to obtain the net absorbance of the mixed reaction solution at the first maximum absorption wavelength;

Subtracting the first absorbance and the third absorbance to obtain the net absorbance of the simulated sample at the first maximum absorption wavelength;

A ratio operation is performed on the net absorbances of the mixed reaction solution and the simulated sample at the first maximum absorption wavelength to obtain the first net absorbance coefficient.

In an optional embodiment, according to the second absorbance, the sixth absorbance and the fourth absorbance, calculate the relative value of the mixed reaction solution to the simulated reagent under the sample reagent mixing operation The steps of the second net absorptivity include:

Subtracting the sixth absorbance and the fourth absorbance to obtain the net absorbance of the mixed reaction solution at the second maximum absorption wavelength;

Subtracting the second absorbance and the fourth absorbance to obtain the net absorbance of the simulated reagent at the second maximum absorption wavelength;

The ratio calculation is performed on the net absorbance of the mixed reaction solution and the simulated reagent at the second maximum absorption wavelength to obtain the second net absorbance coefficient.

In an optional embodiment, the closer the mixing effect evaluation parameter is to the volume ratio between the simulated sample and the simulated reagent corresponding to the mixed reaction solution, the better the mixing effect of the sample reagent mixing operation is .

In a second aspect, the application provides a sample mixing simulation test device, the device includes:

A simulated sample absorbance acquisition module, configured to acquire the first absorbance of the simulated sample matched with the viscosity of the sample to be tested at the first maximum absorption wavelength corresponding to the first fluorescent dye, wherein the simulated sample is dissolved by the first fluorescent dye to the target obtained in viscous solvents;

The simulated reagent absorbance acquisition module is used to acquire the second absorbance of the simulated reagent matched with the preset reagent viscosity at the second maximum absorption wavelength corresponding to the second fluorescent dye, wherein the simulated reagent is dissolved by the second fluorescent dye to the obtained in the target viscous solvent;

a viscous solvent absorbance acquisition module, configured to obtain the third absorbance and the fourth absorbance corresponding to the target viscous solvent at the first maximum absorption wavelength and the second maximum absorption wavelength respectively;

The light absorption acquisition module of mixing operation is used to obtain the mixed reaction solution obtained by the sample reagent mixing operation of the simulated sample and the simulated reagent corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength respectively The fifth absorbance and sixth absorbance;

The mixing effect evaluation and analysis module is used to perform data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance and the sixth absorbance, and obtain The mixing effect evaluation parameter matched with the mixing operation of the sample reagent.

In an optional embodiment, the mixing effect evaluation and analysis module includes:

a first net absorbance calculation sub-module, configured to calculate relative to the simulated sample of the mixed reaction solution under the sample reagent mixing operation according to the first absorbance, the fifth absorbance and the third absorbance The first net absorption coefficient of ;

The second net absorbance calculation sub-module is configured to calculate the relative value of the mixed reaction solution to the simulated reagent under the sample reagent mixing operation according to the second absorbance, the sixth absorbance and the fourth absorbance The second net absorption coefficient of ;

The mixing effect evaluation sub-module is configured to perform a ratio operation on the first net light absorption effect coefficient and the second net light absorption effect coefficient to obtain the mixing effect evaluation parameters matching the mixing operation of the sample reagent.

In an optional embodiment, the closer the mixing effect evaluation parameter is to the volume ratio between the simulated sample and the simulated reagent corresponding to the mixed reaction solution, the better the mixing effect of the sample reagent mixing operation is .

In a third aspect, the present application provides a sample mixing device, comprising a processor and a memory, wherein the memory stores a computer program that can be executed by the processor, and the processor can execute the computer program to achieve The sample mixing simulation test method described in any one of the preceding embodiments.

In a fourth aspect, the present application provides a storage medium on which a computer program is stored, and when the computer program is executed by a processor, implements the sample mixing simulation test method described in any one of the foregoing embodiments.

In this case, the beneficial effects of the embodiments of the present application include the following:

The present application obtains the first absorbance of the simulated sample that matches the viscosity of the sample to be tested at the first maximum absorption wavelength of the autofluorescent material, and the simulated reagent that matches the viscosity of the preset reagent at the second maximum absorption wavelength of the autofluorescent material. The second absorbance, the third absorbance and the fourth absorbance corresponding to the target viscous solvent at the first maximum absorption wavelength and the second maximum absorption wavelength, respectively, at the simulated reagent and the simulated sample, and the simulated sample and the simulated reagent through the sample reagent The mixed reaction solution after the mixing operation has the fifth absorbance and the sixth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength respectively, and then the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, The fifth absorbance and the sixth absorbance are used for data analysis, and the mixing effect evaluation parameters that match the mixing operation of the sample reagents are obtained, so that the simulated samples and simulated reagents that actually participate in the mixing are used to replace the samples to be tested and the corresponding reagents, avoiding the need to be tested. The meaningless loss of samples and corresponding reagents, and the specific mixing effect of the mixing operation of the sample to be tested and the preset reagents is effectively and quantitatively evaluated through the mixing effect evaluation parameter, which is convenient for testing the mixing of sample reagents in automated sample detection equipment. The performance also helps the testing personnel to adjust and optimize the operation details when manually mixing the sample reagents, so that the final testing results are accurate and reliable.

In order to make the above-mentioned objects, features and advantages of the present application more obvious and easy to understand, the preferred embodiments are exemplified below, and are described in detail as follows in conjunction with the accompanying drawings.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present application more clearly, the following drawings will briefly introduce the drawings that need to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore do not It should be regarded as a limitation of the scope, and for those of ordinary skill in the art, other related drawings can also be obtained according to these drawings without any creative effort.

1 is a schematic diagram of the composition of the sample mixing equipment provided in the embodiment of the application;

2 is a schematic flowchart of a sample mixing simulation test method provided in the embodiment of the present application;

3 is a schematic diagram of the absorbance distribution of the fifth and sixth absorbances under the mixing operation of the sample reagents with different mixing durations provided in the embodiment of the present application;

FIG. 4 is a schematic flowchart of the sub-steps included in step S250 shown in FIG. 2;

5 is a schematic table of parameter distribution of the mixing effect evaluation parameters under the mixing operation of sample reagents with different mixing durations provided in the embodiment of the present application;

6 is a schematic diagram of the composition of the sample mixing simulation test device provided by the embodiment of the present application;

FIG. 7 is a schematic diagram of the composition of the mixing effect evaluation and analysis module in FIG. 6 .

Icon: 10-sample mixing equipment; 11-memory; 12-processor; 13-communication unit; 14-mixing components; 100-sample mixing simulation test device; 110-simulated sample absorption acquisition module; 120- Simulated reagent absorbance acquisition module; 130-viscous solvent absorbance acquisition module; 140-mixing operation absorbance acquisition module; 150-mixing effect evaluation and analysis module; 151-first net absorbance calculation sub-module; 152-second net absorbance calculator Module; 153-Mixing effect evaluation sub-module.

Detailed ways

In order to make the purposes, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments It is a part of the embodiments of the present application, but not all of the embodiments. The components of the embodiments of the present application generally described and illustrated in the drawings herein may be arranged and designed in a variety of different configurations.

Thus, the following detailed description of the embodiments of the application provided in the accompanying drawings is not intended to limit the scope of the application as claimed, but is merely representative of selected embodiments of the application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present application.

It should be noted that like numerals and letters refer to like items in the following figures, so once an item is defined in one figure, it does not require further definition and explanation in subsequent figures.

In the description of this application, it is to be understood that relational terms such as the terms "first" and "second" etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require Or imply that there is any such actual relationship or order between these entities or operations. Moreover, the terms "comprising", "comprising" or any other variation thereof are intended to encompass a non-exclusive inclusion such that a process, method, article or device that includes a list of elements includes not only those elements, but also includes not explicitly listed or other elements inherent to such a process, method, article or apparatus. Without further limitation, an element qualified by the phrase "comprising a..." does not preclude the presence of additional identical elements in a process, method, article or apparatus that includes the element. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood in specific situations.

Through painstaking research, the applicant found that the existing technical solutions for evaluating the mixing effect of sample reagents usually include: (1) Subjective qualitative evaluation of the mixing effect by visual means, and it is virtually impossible to quantitatively evaluate the pros and cons of the specific mixing effect. (2) From the perspective of clinical testing, the whole machine is used to perform reverse verification tests on the test results of samples under different degrees of mixing operation, so as to realize the indirect method of the specific mixing effect. Evaluation, the whole process needs to cause the loss of a large number of human samples and in vitro detection reagents.

To this end, the applicant has developed a sample mixing simulation test method and device, a sample mixing device and a storage medium, which can effectively prevent the sample to be tested and the corresponding reagent from being lost. Quantitative evaluation of the specific mixing effect of the mixing operation of the reagents is convenient for testing the mixing performance of the sample reagents of the automated sample testing equipment, and also helps the testing personnel to adjust and optimize the operation details when manually mixing the sample reagents. The test results are accurate and reliable.

Some embodiments of the present application will be described in detail below with reference to the accompanying drawings. The following embodiments and features of the embodiments may be combined with each other without conflict.

Please refer to FIG. 1 . FIG. 1 is a schematic diagram of the composition of the sample mixing device 10 provided by the embodiment of the present application. In the embodiment of the present application, the sample mixing device 10 can perform a mixing operation on the sample to be processed and the reagent to be processed, and perform a specific mixing effect on the sample to be processed and the reagent to be processed. Quantitative evaluation makes it easy for the testing personnel to adjust the details of the mixing operation for the samples to be processed and the reagents to be processed, so that the final detection results corresponding to the samples to be processed and the reagents to be processed are accurate and reliable.

In this embodiment, the sample mixing device 10 may include a memory 11 , a processor 12 , a communication unit 13 , a mixing component 14 and a sample mixing simulation test device 100 . The memory 11 , the processor 12 , the communication unit 13 and the mixing components 14 are directly or indirectly electrically connected to each other to realize data transmission or interaction. For example, the memory 11 , the processor 12 , the communication unit 13 and the mixing component 14 can be electrically connected to each other through one or more communication buses or signal lines.

In this embodiment, the memory 11 may be, but not limited to, a random access memory (Random Access Memory, RAM), a read only memory (Read Only Memory, ROM), a programmable read only memory (Programmable Read-Only Memory, PROM), Erasable Programmable Read-Only Memory (EPROM), Electrically Erasable Programmable Read-Only Memory (Electric Erasable Programmable Read-Only Memory, EEPROM), etc. Wherein, the memory 11 is used for storing a computer program, and the processor 12 can execute the computer program correspondingly after receiving the execution instruction. In addition, the memory 11 is also used to store the specific calculation formula of the mixing effect evaluation parameter, wherein the mixing effect evaluation parameter is used to represent the specific mixing effect of the corresponding sample and the corresponding reagent under the current mixing operation. situation, wherein the closer the mixing effect evaluation parameter is to the volume ratio between the sample and the reagent involved in the mixing operation, the better the mixing effect of the mixing operation is.

In this embodiment, the processor 12 may be an integrated circuit chip with signal processing capability. The processor 12 may be a general-purpose processor, including a central processing unit (Central Processing Unit, CPU), a graphics processing unit (Graphics Processing Unit, GPU), a network processor (Network Processor, NP), a digital signal processor (DSP) ), an application specific integrated circuit (ASIC), an off-the-shelf programmable gate array (FPGA) or other programmable logic device, at least one of a discrete gate or transistor logic device, a discrete hardware component. The general-purpose processor may be a microprocessor, or the processor may also be any conventional processor, etc., and may implement or execute the methods, steps, and logical block diagrams disclosed in the embodiments of this application.

In this embodiment, the communication unit 13 is configured to establish a communication connection between the sample mixing device 10 and other electronic devices through a network, and to send and receive data through the network, where the network includes a wired communication network and wireless communication networks. For example, the sample mixing device 10 may receive the mixing control instruction sent by the control device through the communication unit 13, and perform the corresponding mixing operation according to the mixing duration included in the mixing control instruction.

In this embodiment, the mixing component 14 is used for the mixing function of the sample mixing device 10 , wherein the mixing component 14 may include one or more of a stirring component, an oscillating component, and an ultrasonic component. kind of combination.

In this embodiment, the sample mixing simulation test device 100 includes at least one software that can be stored in the memory 11 in the form of software or firmware or can be solidified in the operating system of the sample mixing device 10 functional module. The processor 12 may be configured to execute executable modules stored in the memory 11 , such as software function modules and computer programs included in the sample mixing simulation test apparatus 100 . The sample mixing device 10 can simulate the specific mixing of the sample to be tested and the corresponding reagent while avoiding the meaningless loss of the sample to be tested and the corresponding reagent through the sample mixing simulation test device 100. Quantitative evaluation of the homogenization effect is convenient for testing the sample reagent mixing performance of the automated sample detection equipment, and also helps the testing personnel to adjust and optimize the operation details when manually mixing the sample reagents, so that the final test results are accurate and reliable.

It can be understood that the block diagram shown in FIG. 1 is only a schematic diagram of the composition of the sample mixing device 10 , and the sample mixing device 10 may further include more or less than those shown in FIG. 1 . components, or have a different configuration than that shown in Figure 1. Each component shown in FIG. 1 may be implemented in hardware, software, or a combination thereof.

In the present application, in order to ensure that the sample mixing device 10 can effectively quantify the specific mixing effect of the mixing operation of the sample to be tested and the corresponding reagent while avoiding the meaningless loss of the sample to be tested and the corresponding reagent It is convenient for the testing personnel to adjust the details of the mixing operation for the sample to be tested and the preset reagent, so that the final test result is accurate and reliable. The embodiment of the present application provides a sample mixing simulation test method to achieve the aforementioned purpose. The following is a detailed description of the sample mixing simulation test method provided in this application.

Please refer to FIG. 2 , which is a schematic flowchart of a sample mixing simulation test method provided by an embodiment of the present application. In the embodiment of the present application, the sample mixing simulation test method may include steps S210 to S250.

Step S210 , obtaining the first absorbance at the first maximum absorption wavelength corresponding to the first fluorescent dye of the simulated sample that matches the viscosity of the sample to be tested, wherein the simulated sample is obtained by dissolving the first fluorescent dye into the target viscous solvent.

In this embodiment, the viscosities of the simulated sample and the sample to be tested are kept consistent, and the simulated sample is obtained by dissolving the first fluorescent material into a target viscous solvent, and the target viscous solvent is a specific concentration of a highly viscous solvent solvent. The first maximum absorption wavelength is used to represent the light wavelength used by the first fluorescent dye under the condition of its own maximum absorbance. Wherein, the first fluorescent dye can be any one of rhodamine B, methyl orange, methylene blue, gentian violet and other dyes. In an implementation of this example, Rhodamine B can be used as the first fluorescent dye, and glycerol diluent can be used as the target viscous solvent, then the simulated sample needs to be placed at the maximum absorption wavelength (475nm) of Rhodamine B. Under the illumination, the first absorbance of the simulated sample under the illumination at 475 nm was determined to be 1.81.

Step S220, acquiring the second absorbance of the simulated reagent matching the preset reagent viscosity at the second maximum absorption wavelength corresponding to the second fluorescent dye, wherein the simulated reagent is obtained by dissolving the second fluorescent dye into the target viscous solvent.

In this embodiment, the viscosity of the simulated reagent is consistent with that of the preset reagent, the preset reagent is one or more reagents that need to be added to the sample to be tested when the sample is detected, and the simulated reagent adopts the first The second fluorescent material is obtained by dissolving it into the target viscous solvent, and the second fluorescent material and the first fluorescent material are different from each other. The second maximum absorption wavelength is used to represent the light wavelength used by the second fluorescent dye under the condition of its own maximum absorbance. Wherein, the second fluorescent dye can also be any one of rhodamine B, methyl orange, methylene blue, gentian violet and other dyes, but the first fluorescent dye and the second fluorescent dye are different from each other. In one implementation of this embodiment, the orange-red G pigment can be used as the second fluorescent dye, and the glycerol diluent can be used as the target viscous solvent, and the simulated reagent needs to be placed at the maximum absorption wavelength (554nm) of the orange-red G pigment. Illumination, and the second absorbance of the simulated reagent under 554 nm illumination was determined to be 0.07.

Step S230, acquiring the third absorbance and the fourth absorbance corresponding to the target viscous solvent at the first maximum absorption wavelength and the second maximum absorption wavelength, respectively.

In this embodiment, the target viscous solvent shared by the simulated reagent and the simulated sample can be placed under the light of the first maximum absorption wavelength of the first fluorescent dye, and the target viscous solvent can be measured at the first maximum absorption wavelength. The absorbance under the light of the wavelength (ie the third absorbance), and the target viscous solvent can be placed under the light of the second maximum absorption wavelength of the second fluorescent dye, and the target viscous solvent is measured at the second maximum absorption wavelength. The absorbance under light (ie the fourth absorbance). In an implementation of this embodiment, when using glycerol diluent as the target viscous solvent, the third absorbance of the target viscous solvent under the illumination of the maximum absorption wavelength (475 nm) of Rhodamine B is 0.02, and the target viscous solvent is at The fourth absorbance was 1.93 when placed under the light of the maximum absorption wavelength (554nm) of the orange-red G pigment.

Step S240, acquiring the fifth absorbance and the sixth absorbance corresponding to the first maximum absorption wavelength and the second maximum absorption wavelength of the mixed reaction solution obtained by performing the sample-reagent mixing operation with the simulated sample and the simulated reagent, respectively.

In this embodiment, after determining the simulated sample that matches the viscosity of the sample to be tested and the simulated reagent that matches the viscosity of the preset reagent, the simulated sample can be used to replace the sample to be tested and the simulated reagent can be used to replace all the samples. The preset reagent is extracted, a certain volume (V ₂ ) of simulated sample and a certain volume (V ₁ ) of simulated reagent are extracted and added to the automated sample detection equipment, and the sample reagent mixing operation is performed for a specific length of time according to the set mixing parameters to obtain The corresponding mixed reaction solution; similarly, a certain volume (V ₂ ) of simulated samples and a certain volume (V ₁ ) of simulated reagents can also be extracted and added to the reaction container of the mixing component 14 of the sample mixing device 10 , the sample mixing device 10 has the same hardware structure and parameter settings as the sample mixing module (such as the oscillation module) of the automatic sample detection device, and can simulate the function of the sample mixing module of the automatic sample detection device. The sample mixing device 10 performs a sample reagent mixing operation with a specific mixing time in the reaction container according to the set mixing parameters, and obtains a corresponding mixed reaction solution, so as to characterize the sample to be tested and the current mixed reaction solution. The specific mixing effect status of the preset reagents under the current sample reagent mixing operation, so as to timely verify the sample mixing performance of the automated sample detection equipment, and ensure that the sample detection results of the automated sample detection equipment are accurate and effective.

Further, after obtaining the mixed reaction solution processed by the current sample reagent mixing operation, the mixed reaction solution can be placed under the light of the first maximum absorption wavelength of the first fluorescent dye to measure the concentration of the mixed reaction solution in the first fluorescent dye. The absorbance under the illumination of the maximum absorption wavelength (that is, the fifth absorbance), and the mixed reaction solution can be placed under the illumination of the second maximum absorption wavelength of the second fluorescent dye, and the mixed reaction solution is measured at the second maximum absorption. The absorbance under the light of the wavelength (ie, the sixth absorbance).

In this process, it is worth noting that the mixing operation of sample reagents in the same execution step often varies with the mixing time, resulting in different specific mixing effects at the end. The glycerol diluent was used as the target viscous solvent, Rhodamine B was used as the first fluorescent dye, and the orange-red G pigment was used as the second fluorescent dye. At this time, the corresponding simulated samples and simulated reagents were mixed for different mixing time. The specific numerical values of each of the fifth absorbance and the sixth absorbance of the mixed reaction solution are shown in FIG. 3 . As shown in Figure 3, the time unit corresponding to "mixing time" is 1min. When the mixing time is 8min and above, the corresponding fifth absorbance and sixth absorbance will remain unchanged; when the mixing time gradually increases and is less than At 8min, the corresponding fifth absorbance will gradually decrease, and the corresponding sixth absorbance will gradually increase.

Step S250: Perform data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance, and the sixth absorbance to obtain a mixing effect evaluation parameter matching the sample reagent mixing operation.

In this embodiment, the calculation formula of the mixing effect evaluation parameter is as follows:

Among them, H is used to represent the mixing effect evaluation parameter of the mixed reaction solution under the current sample reagent mixing operation, A is used to represent the fifth absorbance of the mixed reaction solution under the current sample reagent mixing operation, B is used to represent the current sample The sixth absorbance of the mixed reaction solution under the reagent mixing operation, A ₀ is used to represent the third absorbance of the target viscous solvent at the first maximum absorption wavelength, and B ₀ is used to represent the target viscous solvent at the second maximum absorption wavelength. The fourth absorbance, A ₁ is used to represent the first absorbance of the simulated sample at the first maximum absorption wavelength, and B ₁ is used to represent the second absorbance of the simulated sample at the second maximum absorption wavelength. Wherein, the closer the value of the mixing effect evaluation parameter is to the volume ratio (V ₂ /V ₁ ) between the simulated sample and the simulated reagent, the better the mixing effect of the current sample reagent mixing operation is.

In this case, the sample mixing device 10 can use the calculation formula of the above-mentioned mixing effect evaluation parameter to calculate the first absorbance, the second absorbance, the third absorbance, and the fourth absorbance. , Perform data analysis on the fifth absorbance and the sixth absorbance, and obtain the mixing effect evaluation parameters matching the mixing operation of the sample to be tested, the preset reagent and the current sample reagent, so as to realize the difference between the sample to be tested and the sample reagent. The specific mixing effect of the mixing operation of the preset reagents can be effectively and quantitatively evaluated, which is convenient for testing the mixing performance of the sample reagents of the automated sample testing equipment, and also helps the testing personnel to adjust and optimize the operation details when manually mixing the sample reagents. , so that the final detection result is accurate and reliable.

Therefore, in the present application, by performing the above steps S210 to S250, the simulated samples and simulated reagents that actually participate in the mixing can be used to replace the samples to be tested and the preset reagents, respectively, so as to avoid the meaningless loss of the samples to be tested and the preset reagents, and The specific mixing effect of the mixing operation between the sample to be tested and the preset reagent can be effectively and quantitatively evaluated by the mixing effect evaluation parameter, which is convenient for testing the mixing performance of the sample reagents of the automated sample testing equipment, and also helps the testing personnel to manually perform Adjust and optimize the operation details during the sample reagent mixing operation, so that the final test results are accurate and reliable.

Optionally, please refer to FIG. 4 , which is a schematic flowchart of the sub-steps included in step S250 shown in FIG. 2 . In this embodiment, the step S250 may include sub-steps S251 to S253.

Sub-step S251, according to the first absorbance, the fifth absorbance and the third absorbance, calculate the first net absorbance coefficient of the mixed reaction solution relative to the simulated sample under the sample reagent mixing operation.

In this embodiment, the first net absorbance coefficient is used to represent the multiple of the absorbance of the mixed reaction solution relative to the simulated sample under the condition that the absorbance interference of the target viscous solvent is excluded. The first absorbance, the fifth absorbance and the third absorbance, the step of calculating the first net absorbance coefficient of the mixed reaction solution relative to the simulated sample under the sample reagent mixing operation may include:

Subtracting the fifth absorbance and the third absorbance to obtain the net absorbance of the mixed reaction solution at the first maximum absorption wavelength;

Subtracting the first absorbance and the third absorbance to obtain the net absorbance of the simulated sample at the first maximum absorption wavelength;

A ratio calculation is performed on the net absorbance of the mixed reaction solution and the simulated sample at the first maximum absorption wavelength to obtain the first net absorbance coefficient.

At this time, the calculation formula of the first net light absorption coefficient is the molecular expression in the calculation formula of the mixing effect evaluation parameter.

Sub-step S252, according to the second absorbance, the sixth absorbance and the fourth absorbance, calculate the second net absorbance coefficient of the mixed reaction solution relative to the simulated reagent under the sample reagent mixing operation.

In this embodiment, the second net absorption coefficient is used to represent the multiple of the absorbance of the mixed reaction solution relative to the simulated reagent under the condition that the interference of the absorbance of the target viscous solvent is excluded. The second absorbance, the sixth absorbance and the fourth absorbance, the step of calculating the second net absorbance coefficient of the mixed reaction solution relative to the simulated reagent under the operation of mixing the sample reagents may include:

Subtracting the sixth absorbance and the fourth absorbance to obtain the net absorbance of the mixed reaction solution at the second maximum absorption wavelength;

Subtracting the second absorbance and the fourth absorbance to obtain the net absorbance of the simulated reagent at the second maximum absorption wavelength;

The ratio calculation is performed on the net absorbance of the mixed reaction solution and the simulated reagent at the second maximum absorption wavelength to obtain the second net absorbance coefficient.

At this time, the calculation formula of the second net light absorption coefficient is the denominator expression in the calculation formula of the above-mentioned mixing effect evaluation parameter.

In sub-step S253, a ratio operation is performed on the first net light absorption effect coefficient and the second net light absorption effect coefficient, and a mixing effect evaluation parameter matching the mixing operation of the sample reagent is obtained.

In this embodiment, after the sample mixing device 10 calculates the first net light absorption coefficient and the second net light absorption coefficient corresponding to the mixed reaction solution of the current sample reagent mixing operation, it can be The ratio calculation of the first net light absorption coefficient and the second net light absorption coefficient is performed to obtain the correspondingly matched mixing effect evaluation parameters under the current sample reagent mixing operation.

In this process, it is worth noting that the mixing operation of sample reagents in the same execution step often varies with the mixing time, resulting in different evaluation parameters for the final mixing effect. The glycerol diluent was used as the target viscous solvent, Rhodamine B was used as the first fluorescent dye, and the orange-red G pigment was used as the second fluorescent dye. Six absorbance, at this time, the specific values of the mixing effect evaluation parameters corresponding to the simulated sample and the simulated reagent under the sample reagent mixing operation of different mixing time are shown in Figure 5. As shown in Figure 5, the time unit corresponding to "mixing time" is 1min. When the mixing time is 8min or more, the corresponding mixing effect evaluation parameters will remain unchanged. At this time, the corresponding mixing effect is the best; When the mixing time gradually increases and is less than 8min, the corresponding mixing effect evaluation parameters will gradually decrease, and the corresponding mixing effect will gradually become better.

Therefore, the present application can effectively quantitatively evaluate the specific mixing effect of the sample to be tested and the preset reagent under the current sample reagent mixing operation by performing the above sub-steps S251 to S253, which is convenient for inspection automation. The sample reagent mixing performance of the sample testing equipment also helps the testing personnel to adjust and optimize the operation details when manually mixing the sample reagents, so that the final testing results are accurate and reliable.

In the present application, in order to ensure that the sample mixing device 10 can perform the above-mentioned sample mixing simulation test method through the sample mixing simulation test device 100, the present application simulates the sample mixing by the sample mixing simulation test device 100. 100 implements the foregoing functions by dividing the functional modules. The specific composition of the sample mixing simulation test device 100 provided by the present application will be described below accordingly.

Please refer to FIG. 6 . FIG. 6 is a schematic diagram of the composition of the sample mixing simulation test device 100 provided by the embodiment of the present application. In the embodiment of the present application, the sample mixing simulation test device 100 may include a simulated sample light absorption acquisition module 110, a simulated reagent light absorption acquisition module 120, a viscous solvent light absorption acquisition module 130, a mixing operation light absorption acquisition module 140, and a mixing operation light absorption acquisition module 140. Effect evaluation analysis module 150 .

The simulated sample absorbance acquisition module 110 is configured to acquire the first absorbance of the simulated sample matched with the viscosity of the sample to be tested at the first maximum absorption wavelength corresponding to the first fluorescent dye, wherein the simulated sample is dissolved by the first fluorescent dye to in the target viscous solvent.

The simulated reagent absorbance acquisition module 120 is used to obtain the second absorbance of the simulated reagent matched with the preset reagent viscosity at the second maximum absorption wavelength corresponding to the second fluorescent dye, wherein the simulated reagent is dissolved by the second fluorescent dye to in the target viscous solvent.

The viscous solvent absorbance acquisition module 130 is configured to acquire the third absorbance and the fourth absorbance corresponding to the target viscous solvent at the first maximum absorption wavelength and the second maximum absorption wavelength, respectively.

The light absorption acquisition module 140 for mixing operation is used to obtain the mixed reaction solution obtained by the sample reagent mixing operation of the simulated sample and the simulated reagent at the first maximum absorption wavelength and the second maximum absorption wavelength, respectively. Corresponding fifth absorbance and sixth absorbance.

The mixing effect evaluation and analysis module 150 is configured to perform data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance and the sixth absorbance, A mixing effect evaluation parameter matching the mixing operation of the sample reagent is obtained. Wherein, the closer the mixing effect evaluation parameter is to the volume ratio between the simulated sample and the simulated reagent corresponding to the mixed reaction solution, the better the mixing effect of the sample reagent mixing operation is.

Optionally, please refer to FIG. 7 , which is a schematic diagram of the composition of the mixing effect evaluation and analysis module 150 in FIG. 6 . In this embodiment, the mixing effect evaluation and analysis module 150 may include a first net light absorption calculation sub-module 151 , a second net light absorption calculation sub-module 152 and a mixing effect evaluation sub-module 153 .

The first net absorbance calculation sub-module 151 is configured to calculate the relative value of the mixed reaction solution to the simulation under the sample reagent mixing operation according to the first absorbance, the fifth absorbance and the third absorbance The first net absorptivity coefficient of the sample.

The second net absorbance calculation sub-module 152 is configured to calculate, according to the second absorbance, the sixth absorbance and the fourth absorbance, the relative value of the mixed reaction solution to the simulation under the sample reagent mixing operation The second net absorption coefficient of the reagent.

The mixing effect evaluation sub-module 153 is configured to perform a ratio calculation on the first net light absorption coefficient and the second net light absorption coefficient to obtain the mixing effect evaluation parameter matching the mixing operation of the sample reagent .

It should be noted that, the basic principles and technical effects of the sample mixing simulation test device 100 provided in the embodiments of the present application are the same as the aforementioned sample mixing simulation test methods. For a brief description, for the parts not mentioned in this embodiment, reference may be made to the above description of the sample mixing simulation test method.

In the embodiments provided in this application, it should be understood that the disclosed apparatus and method may also be implemented in other manners. The apparatus embodiments described above are merely illustrative, eg, the flowcharts and block diagrams in the accompanying drawings illustrate the architecture, functionality and operation of possible implementations of apparatuses, methods and computer program products according to embodiments of the present application. In this regard, each block in the flowchart or block diagrams may represent a module, segment, or portion of code that contains one or more functions for implementing the specified logical function(s) executable instructions. It should also be noted that, in some alternative implementations, the functions noted in the block may occur out of the order noted in the figures. For example, two blocks in succession may, in fact, be executed substantially concurrently, or the blocks may sometimes be executed in the reverse order, depending upon the functionality involved. It is also noted that each block of the block diagrams and/or flowchart illustrations, and combinations of blocks in the block diagrams and/or flowchart illustrations, can be implemented in dedicated hardware-based systems that perform the specified functions or actions , or can be implemented in a combination of dedicated hardware and computer instructions.

In addition, each functional module in each embodiment of the present application may be integrated together to form an independent part, or each module may exist independently, or two or more modules may be integrated to form an independent part. If the functions are implemented in the form of software function modules and sold or used as independent products, they can be stored in a storage medium. Based on such understanding, the technical solutions of the present application can be embodied in the form of software products in essence, or the parts that make contributions to the prior art or the parts of the technical solutions, and the computer software products are stored in a readable storage medium , including several instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the methods described in the various embodiments of the present application. The aforementioned storage medium includes: U disk, removable hard disk, Read-Only Memory (ROM, Read-Only Memory), Random Access Memory (RAM, Random Access Memory), magnetic disk or optical disk and other media that can store program codes.

To sum up, in the sample mixing simulation test method and device, the sample mixing equipment and the storage medium provided by the present application, the present application obtains the simulated sample whose viscosity matches the sample to be tested in the first test of the autofluorescent material. The first absorbance at the maximum absorption wavelength, the second absorbance at the second maximum absorption wavelength of the autofluorescent material by the simulated reagent matching the viscosity of the preset reagent, the target viscous solvent shared at the simulated reagent and the simulated sample at the first maximum The third absorbance and the fourth absorbance corresponding to the absorption wavelength and the second maximum absorption wavelength, respectively, and the first maximum absorption wavelength and the second maximum absorption wavelength of the mixed reaction solution after the simulated sample and the simulated reagent are mixed with the sample reagent. The fifth absorbance and the sixth absorbance corresponding to the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance and the sixth absorbance respectively, and then the data analysis is carried out to obtain the sample reagents that match the mixing operation. Mixing effect evaluation parameters, so that the samples to be tested and preset reagents are replaced by simulated samples and simulated reagents that actually participate in mixing, avoiding the meaningless loss of samples to be tested and preset reagents, and the parameters to be tested are evaluated by mixing effect evaluation parameters. The specific mixing effect of the mixing operation between the sample and the preset reagent can be effectively quantitatively evaluated, which is convenient for testing the mixing performance of the sample reagent of the automated sample detection equipment, and also helps the testing personnel to adjust and optimize the mixing operation of the sample reagent manually. The operation details make the final detection result accurate and reliable.

The above are only various embodiments of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed in the present application, All should be covered within the scope of protection of this application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.

Claims (10)

1. A sample mixing simulation test method is characterized by comprising the following steps:

obtaining a first absorbance of a simulation sample matched with the viscosity of the sample to be detected at a first maximum absorption wavelength corresponding to a first fluorescent dye, wherein the simulation sample is obtained by dissolving the first fluorescent dye into a target viscous solvent;

obtaining a second absorbance of a simulation reagent matched with the viscosity of a preset reagent at a second maximum absorption wavelength corresponding to a second fluorescent dye, wherein the simulation reagent is obtained by dissolving the second fluorescent dye into the target viscous solvent;

acquiring a third absorbance and a fourth absorbance respectively corresponding to the target viscous solvent at the first maximum absorption wavelength and the second maximum absorption wavelength;

acquiring a fifth absorbance and a sixth absorbance which respectively correspond to a mixed reaction solution obtained by uniformly mixing the sample reagent with the simulation sample at the first maximum absorption wavelength and the second maximum absorption wavelength;

and carrying out data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance and the sixth absorbance to obtain a blending effect evaluation parameter matched with the blending operation of the sample reagent.

2. The method of claim 1, wherein the step of analyzing the data of the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance, and the sixth absorbance to obtain a mixing effect assessment parameter matching the sample reagent mixing operation comprises:

calculating a first net absorption coefficient of the mixed reaction liquid relative to the simulated sample under the sample reagent mixing operation according to the first absorbance, the fifth absorbance and the third absorbance;

calculating a second net absorption coefficient of the mixed reaction liquid relative to the simulation reagent under the sample reagent mixing operation according to the second absorbance, the sixth absorbance and the fourth absorbance;

and carrying out ratio operation on the first net absorption coefficient and the second net absorption coefficient to obtain the blending effect evaluation parameter matched with the sample reagent blending operation.

3. The method of claim 2, wherein the step of calculating a first net absorption coefficient of the mixed reaction solution relative to the simulated sample in the sample reagent mixing operation based on the first absorbance, the fifth absorbance, and the third absorbance comprises:

carrying out subtraction operation on the fifth absorbance and the third absorbance to obtain the net absorbance of the mixed reaction liquid at the first maximum absorption wavelength;

carrying out subtraction operation on the first absorbance and the third absorbance to obtain the net absorbance of the simulation sample at the first maximum absorption wavelength;

and carrying out ratio operation on the net absorbance of the mixed reaction liquid and the simulated sample at the first maximum absorption wavelength respectively to obtain the first net absorption action coefficient.

4. The method of claim 2, wherein the step of calculating a second net absorption coefficient of the mixed reaction solution relative to the simulated reagent under the sample reagent mixing operation according to the second absorbance, the sixth absorbance and the fourth absorbance comprises:

carrying out subtraction operation on the sixth absorbance and the fourth absorbance to obtain the net absorbance of the mixed reaction liquid at the second maximum absorption wavelength;

carrying out subtraction operation on the second absorbance and the fourth absorbance to obtain the net absorbance of the simulation reagent at the second maximum absorption wavelength;

and carrying out ratio operation on the net absorbance of the mixed reaction liquid and the simulation reagent at a second maximum absorption wavelength respectively to obtain a second net absorption action coefficient.

5. The method according to any one of claims 1 to 4, wherein the closer the blending effect evaluation parameter is to the volume ratio between the simulated sample and the simulated reagent corresponding to the mixed reaction solution, the better the blending effect of the sample reagent blending operation is.

6. A sample blending simulation test device is characterized by comprising:

the simulated sample light absorption acquisition module is used for acquiring first absorption intensity of a simulated sample matched with the viscosity of the sample to be detected at a first maximum absorption wavelength corresponding to the first fluorescent dye, wherein the simulated sample is obtained by dissolving the first fluorescent dye into a target viscous solvent;

the simulated reagent light absorption acquisition module is used for acquiring second absorbance of a simulated reagent matched with the viscosity of a preset reagent at a second maximum absorption wavelength corresponding to a second fluorescent dye, wherein the simulated reagent is obtained by dissolving the second fluorescent dye into the target viscous solvent;

the viscous solvent light absorption obtaining module is used for obtaining a third absorbance and a fourth absorbance respectively corresponding to the target viscous solvent at the first maximum absorption wavelength and the second maximum absorption wavelength;

a light absorption obtaining module for mixing operation, configured to obtain a fifth absorbance and a sixth absorbance respectively corresponding to a mixed reaction solution obtained by mixing the sample reagent with the simulation sample at the first maximum absorption wavelength and the second maximum absorption wavelength;

and the blending effect evaluation and analysis module is used for carrying out data analysis on the first absorbance, the second absorbance, the third absorbance, the fourth absorbance, the fifth absorbance and the sixth absorbance to obtain blending effect evaluation parameters matched with the blending operation of the sample reagent.

7. The apparatus of claim 6, wherein the blending effect evaluation and analysis module comprises:

the first net absorbance calculation submodule is used for calculating a first net absorbance action coefficient of the mixed reaction liquid relative to the simulation sample under the sample reagent uniformly mixing operation according to the first absorbance, the fifth absorbance and the third absorbance;

the second net light absorption calculation submodule is used for calculating a second net light absorption action coefficient of the mixed reaction liquid relative to the simulation reagent under the sample reagent mixing operation according to the second absorbance, the sixth absorbance and the fourth absorbance;

and the blending effect evaluation submodule is used for carrying out ratio operation on the first net absorption coefficient and the second net absorption coefficient to obtain the blending effect evaluation parameter matched with the sample reagent blending operation.

8. The apparatus according to claim 6 or 7, wherein the more the blending effect evaluation parameter is closer to the volume ratio between the simulated sample and the simulated reagent corresponding to the mixed reaction solution, the better the blending effect of the sample reagent blending operation is.

9. A sample mixing apparatus comprising a processor and a memory, wherein the memory stores a computer program executable by the processor, and the processor executes the computer program to implement the sample mixing simulation test method according to any one of claims 1 to 5.

10. A storage medium having a computer program stored thereon, wherein the computer program, when executed by a processor, implements the specimen mixing simulation test method according to any one of claims 1 to 5.

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