CN114208815A - Sperm diluent suitable for large-scale poultry farm and preparation method thereof - Google Patents
Sperm diluent suitable for large-scale poultry farm and preparation method thereof Download PDFInfo
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- CN114208815A CN114208815A CN202111659131.7A CN202111659131A CN114208815A CN 114208815 A CN114208815 A CN 114208815A CN 202111659131 A CN202111659131 A CN 202111659131A CN 114208815 A CN114208815 A CN 114208815A
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- 244000144977 poultry Species 0.000 title claims abstract description 62
- 239000003085 diluting agent Substances 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 28
- 210000000582 semen Anatomy 0.000 claims abstract description 27
- AGBQKNBQESQNJD-UHFFFAOYSA-N lipoic acid Chemical compound OC(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-N 0.000 claims abstract description 23
- 230000009027 insemination Effects 0.000 claims abstract description 19
- 239000005715 Fructose Substances 0.000 claims abstract description 15
- 229930091371 Fructose Natural products 0.000 claims abstract description 15
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims abstract description 15
- 239000011780 sodium chloride Substances 0.000 claims abstract description 14
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 13
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 13
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 13
- 229960000999 sodium citrate dihydrate Drugs 0.000 claims abstract description 13
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 claims abstract description 12
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 claims abstract description 12
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 claims abstract description 12
- 235000013793 astaxanthin Nutrition 0.000 claims abstract description 12
- 229940022405 astaxanthin Drugs 0.000 claims abstract description 12
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 claims abstract description 12
- 239000001168 astaxanthin Substances 0.000 claims abstract description 12
- 235000019136 lipoic acid Nutrition 0.000 claims abstract description 12
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 12
- 229940016667 resveratrol Drugs 0.000 claims abstract description 12
- 235000021283 resveratrol Nutrition 0.000 claims abstract description 12
- 229960002663 thioctic acid Drugs 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 8
- 238000010790 dilution Methods 0.000 claims description 21
- 239000012895 dilution Substances 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 241000271566 Aves Species 0.000 claims description 3
- 210000003555 cloaca Anatomy 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims 2
- AGBQKNBQESQNJD-SSDOTTSWSA-N (R)-lipoic acid Chemical compound OC(=O)CCCC[C@@H]1CCSS1 AGBQKNBQESQNJD-SSDOTTSWSA-N 0.000 claims 1
- JXIYGIIFGDBDBW-UHFFFAOYSA-N O.O.[Na].C(CC(O)(C(=O)O)CC(=O)O)(=O)O Chemical compound O.O.[Na].C(CC(O)(C(=O)O)CC(=O)O)(=O)O JXIYGIIFGDBDBW-UHFFFAOYSA-N 0.000 claims 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract description 11
- 208000007893 Salpingitis Diseases 0.000 abstract description 10
- 238000009395 breeding Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 230000004720 fertilization Effects 0.000 abstract description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 4
- 210000003101 oviduct Anatomy 0.000 abstract description 4
- 229910052760 oxygen Inorganic materials 0.000 abstract description 4
- 239000001301 oxygen Substances 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract 1
- 230000003385 bacteriostatic effect Effects 0.000 abstract 1
- 241000287828 Gallus gallus Species 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000012447 hatching Effects 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000004321 preservation Methods 0.000 description 4
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 239000003642 reactive oxygen metabolite Substances 0.000 description 2
- 230000019100 sperm motility Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 208000012239 Developmental disease Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 230000007253 cellular alteration Effects 0.000 description 1
- 230000010267 cellular communication Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000009483 enzymatic pathway Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 210000005000 reproductive tract Anatomy 0.000 description 1
- 238000010583 slow cooling Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/126—Physiologically active agents, e.g. antioxidants or nutrients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/124—Disinfecting agents, e.g. antimicrobials
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of poultry breeding, and particularly discloses a sperm diluent suitable for a large-scale poultry farm, wherein each 100ml of the sperm diluent comprises the following components: 0.6-0.8 g of sodium chloride, 1.3-1.6 g of fructose, 0.28-0.34 g of monopotassium phosphate, 1.6-1.7 g of sodium citrate dihydrate, 0.1-0.3 mg of astaxanthin, 0.1-0.2 mg of alpha-lipoic acid and 0.1-0.2 mg of resveratrol. The sperm diluent is suitable for large-scale poultry farms, can protect sperms from being damaged by active oxygen to the greatest extent, is beneficial to the survival of the sperms, has bactericidal and bacteriostatic activity, can effectively protect oviducts, and can reduce the incidence rate of salpingitis; can keep the sperm vitality and improve the fertilization rate, the diluted poultry semen can keep the vitality for a long time, and the method is suitable for the artificial insemination of large-scale poultry farms.
Description
Technical Field
The invention belongs to the technical field of poultry breeding, and particularly relates to a sperm diluent suitable for a large-scale poultry farm and a preparation method thereof.
Background
At present, artificial insemination is the most main breeding mode in chicken farms with intensive cultivation. The artificial insemination is realized by collecting semen of the cock, adding the semen diluent according to a certain dilution ratio to achieve the purpose of increasing the semen volume, and a technician injects the diluted semen into the reproductive tract of the hen by a means of turning the anus of the hen by using a rubber-tipped dropper to complete the insemination process. Sperm are highly sensitive to reactive oxygen species because their plasma membranes are rich in polyunsaturated fatty acids, which are the main targets of oxidation, while their cytoplasm is largely confined to the mid-segment, with few antioxidant mechanisms to provide adequate protection against oxidative damage. Active oxygen in semen may lead to lipid peroxidation, DNA fragmentation, cellular communication and alterations in the enzymatic pathways, which in turn are associated with loss of viability, low fertilization rates or embryo developmental disorders. In the process of collecting the semen of the cock to artificially inseminate the hen, the sperms of the poultry are easily damaged by active oxygen, which causes damage to plasma membranes, thereby reducing the vitality of the sperms. For large-scale poultry farms, there is a long time lag from harvest to hen insemination and the sperm are more vulnerable to reactive oxygen species. Under the conditions of large-scale intensive breeding and artificial insemination of poultry, salpingitis of hen groups occurs occasionally, and a certain negative influence is generated on the artificial insemination process, for example, the insemination is difficult due to the pathological changes of the oviduct, and the sperm is adversely affected by inflammatory factors in the oviduct, so that the egg laying and death and culling of the breeding hens can be directly reduced. In order to deal with the situation, a sperm diluent needs to be developed, which not only needs to provide sperm energy, keep acid-base balance and maintain the balance of internal and external osmotic pressures of the sperm, but also needs to provide additional antioxidant protection, further can reduce the incidence rate of poultry salpingitis, is beneficial to improving the fertilization rate of poultry eggs, and thus increases the economic benefit of a farm.
Disclosure of Invention
The invention aims to provide a sperm diluent suitable for a large-scale poultry farm and a preparation method thereof, which can improve the fertilization rate of poultry, effectively prolong the preservation time and the preservation quality of poultry semen and reduce the occurrence of salpingitis.
To achieve the above objects, the present invention provides a sperm dilution solution suitable for use in a large-scale poultry farm, the sperm dilution solution comprising the following components per 100 ml: 0.6-0.8 g of sodium chloride, 1.3-1.6 g of fructose, 0.28-0.34 g of monopotassium phosphate, 1.6-1.7 g of sodium citrate dihydrate, 0.1-0.3 mg of astaxanthin, 0.1-0.2 mg of alpha-lipoic acid and 0.1-0.2 mg of resveratrol.
Preferably, in the sperm dilution solution suitable for use in large-scale poultry farms, the sperm dilution solution comprises the following components per 100 ml: 0.65g of sodium chloride, 1.5g of fructose, 0.32g of monopotassium phosphate, 1.65g of sodium citrate dihydrate, 0.2mg of astaxanthin, 0.2mg of alpha-lipoic acid and 0.1mg of resveratrol.
Preferably, in the sperm diluent suitable for the large-scale poultry farm, the sperm diluent is adopted to dilute the collected qualified poultry semen by 2-5 times, and then artificial insemination of poultry is performed or the diluted poultry semen is stored.
Preferably, in the sperm diluent suitable for the large-scale poultry farm, the sperm diluent is preheated to 35-40 ℃, and then poultry semen is diluted.
Preferably, in the sperm dilution solution suitable for large-scale poultry farms, the artificial insemination of the poultry is as follows: 0.05-0.1 ml of diluted poultry semen is taken and dropped into a poultry cloaca.
Preferably, in the sperm diluent suitable for large-scale poultry farms, the diluted poultry semen is preserved: and subpackaging the diluted semen in thin tubes, and slowly cooling and balancing in a refrigerator at 4 ℃ for storage.
The preparation method of the sperm diluent suitable for the large-scale poultry farm comprises the steps of weighing sodium chloride, fructose, monopotassium phosphate and sodium citrate dihydrate according to the proportion, adding the weighed substances into 80ml of sterilized distilled water, fully dissolving and uniformly mixing, adjusting the pH value to 7.5-8, adding astaxanthin, alpha-lipoic acid and resveratrol, uniformly mixing, adding sterilized distilled water, and fixing the volume to 100ml to obtain the sperm diluent.
Preferably, in the preparation method of the sperm diluent suitable for the large-scale poultry farm, the sperm diluent is subpackaged and stored at a storage temperature of 4-8 ℃.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention is suitable for the sperm diluent of a large-scale poultry farm, is used for taking fructose as the most main energy substance of sperm, and can directly improve the activity of the sperm; the astaxanthin, the alpha-lipoic acid and the resveratrol are used in a matching way, so that sperms can be protected from being damaged by active oxygen to the greatest extent, the sperm survival is facilitated, the sterilization and bacteriostasis activities are realized, the oviduct can be effectively protected, and the incidence rate of salpingitis can be reduced. The poultry semen is diluted by the sperm diluent, so that the volume of the poultry semen can be enlarged, the insemination number of the inseminated hens can be increased, the sperm energy is provided, the acid-base balance is kept, the balance of the osmotic pressure inside and outside the sperm is maintained, the anti-oxidation protection is provided, the sperm vitality is kept, the fertilization capability is improved, the incidence rate of salpingitis is reduced, the in-vitro preservation time of the poultry semen is prolonged, the excellent poultry semen can be utilized to the maximum extent, the feeding cost can be saved, the economic benefit can be improved, and the poultry semen diluent plays a leading role in the breeding of chicken flocks.
2. The sperm diluent suitable for the large-scale poultry farm has relatively simple components, a preparation method, lower cost and convenient operation, and the diluted poultry semen can keep vitality for a longer time and is suitable for artificial insemination of the large-scale poultry farm.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Example 1
A sperm dilution suitable for use in large scale poultry farms, comprising per 100ml of sperm dilution the following components: 0.65g of sodium chloride, 1.5g of fructose, 0.32g of monopotassium phosphate, 1.65g of sodium citrate dihydrate, 0.2mg of astaxanthin, 0.2mg of alpha-lipoic acid and 0.1mg of resveratrol. Under the aseptic condition, weighing sodium chloride, fructose, monopotassium phosphate and sodium citrate dihydrate according to the proportion, adding the weighed substances into 80ml of sterilized distilled water, fully dissolving and uniformly mixing, adjusting the pH to 7.5-8, adding astaxanthin, alpha-lipoic acid and resveratrol, uniformly mixing, adding sterilized distilled water, and fixing the volume to 100ml to obtain the sperm diluent.
Example 2
A sperm dilution suitable for use in large scale poultry farms, comprising per 100ml of sperm dilution the following components: 0.6g of sodium chloride, 1.6g of fructose, 0.30g of monopotassium phosphate, 1.6g of sodium citrate dihydrate, 0.25mg of astaxanthin, 0.1mg of alpha-lipoic acid and 0.15mg of resveratrol. The formulation method was the same as in example 1.
Comparative example 1
A sperm dilution comprising, per 100ml of sperm dilution: 0.65g of sodium chloride, 1.5g of fructose, 0.32g of monopotassium phosphate and 1.65g of sodium citrate dihydrate.
Comparative example 2
A sperm dilution suitable for use in large scale poultry farms, comprising per 100ml of sperm dilution the following components: 0.65g of sodium chloride, 1.5g of fructose, 0.32g of monopotassium phosphate, 1.65g of sodium citrate dihydrate and 0.5mg of astaxanthin.
Comparative example 3
A sperm dilution suitable for use in large scale poultry farms, comprising per 100ml of sperm dilution the following components: 0.65g of sodium chloride, 1.5g of fructose, 0.32g of monopotassium phosphate, 1.65g of sodium citrate dihydrate and 0.5mg of alpha-lipoic acid.
Comparative example 4
A sperm dilution suitable for use in large scale poultry farms, comprising per 100ml of sperm dilution the following components: 0.65g of sodium chloride, 1.5g of fructose, 0.32g of monopotassium phosphate, 1.65g of sodium citrate dihydrate and 0.5mg of resveratrol.
Testing the poultry semen preserving Activity
Respectively preparing the fresh sperm diluent of the examples 1-2 and the fresh sperm diluent of the comparative examples 1-4, preheating the diluent in a water bath to 40 ℃, pouring the sperm diluent into a sperm collecting cup filled with qualified fresh chicken sperm, diluting the chicken sperm by 2 times, shaking and mixing the diluted chicken sperm and the diluted chicken sperm gently to obtain the diluted chicken sperm, respectively filling the diluted chicken sperm into thin tubes, and storing the chicken sperm in a refrigerator at 4 ℃ for slow cooling and balancing. The sperm motility rate is checked every 24h, and the specific results are shown in the table 1.
TABLE 1 cryo-preserved sperm motility (%)
As can be seen from Table 1, the survival rate of the sperm cells of the example group is significantly higher than that of the comparative example group, the survival rate of the sperm cells is more than 50% when the sperm cells of the example group are stored at low temperature for 5 days, and the sperm cell diluent of the invention can maintain the sperm cell activity and prolong the in vitro preservation time of the sperm cells of the birds.
Experiment two is used for artificial insemination of breeding hens
Preparing sperm diluent of example 1 and comparative examples 1-4, preheating the sperm diluent to 40 ℃ in water bath, diluting the collected qualified sperm by 2 times by using the sperm diluent, after shaking gently and fully, respectively carrying out artificial insemination in four henhouses (the number of 1000 hens in each hen, the variety, the age of the day and other conditions are consistent) of the chicken farm, adopting a cloaca eversion insemination method, wherein the input amount of the sperm diluent of each hen diluted every day is 0.05ml (every 5 days is a period), investigating the number of dead hens caused by salpingitis after one week of insemination, the fertility rate and the hatching rate of each hen house each day are 1 month, and carrying out statistical calculation on the salpingitis death and culling rate, the average fertilization rate of the hatching months and the average hatching egg rate of the breeding hens, wherein the results are shown in table 2.
Table 2 shows the performance data of the hens after artificial insemination in each experimental group
As can be seen from Table 2, the mortality and culling rate of the hen salpingitis in the group of example 1 is 0.45%, which is significantly lower than that in the groups of comparative examples 1 to 4, and the average fertilization rate per month and the average hatching rate per month are 94.63% and 94.48%, which are significantly higher than those in the groups of comparative examples 1 to 4, which shows that the sperm diluent of the present invention not only can improve the fertilization rate, but also can achieve the effects of sterilization, salpingitis prevention and hatching rate improvement.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (8)
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111659131.7A CN114208815A (en) | 2021-12-30 | 2021-12-30 | Sperm diluent suitable for large-scale poultry farm and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111659131.7A CN114208815A (en) | 2021-12-30 | 2021-12-30 | Sperm diluent suitable for large-scale poultry farm and preparation method thereof |
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| CN114208815A true CN114208815A (en) | 2022-03-22 |
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|---|---|---|---|---|
| CN115005200A (en) * | 2022-07-26 | 2022-09-06 | 吉林大学 | A kind of semen dilution for livestock and poultry and preparation method thereof |
| CN116918799A (en) * | 2023-06-29 | 2023-10-24 | 吉林省农业科学院 | A frozen diluent of chicken semen containing lipoic acid and its preparation method |
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