CN103816198A - Safflower extract and application thereof - Google Patents
Safflower extract and application thereof Download PDFInfo
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- CN103816198A CN103816198A CN201210469067.0A CN201210469067A CN103816198A CN 103816198 A CN103816198 A CN 103816198A CN 201210469067 A CN201210469067 A CN 201210469067A CN 103816198 A CN103816198 A CN 103816198A
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- flos carthami
- extract
- ethanol
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- carthami extract
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- 229940119485 safflower extract Drugs 0.000 title abstract 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 37
- 239000000284 extract Substances 0.000 claims abstract description 34
- 210000002997 osteoclast Anatomy 0.000 claims abstract description 29
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 21
- 208000001132 Osteoporosis Diseases 0.000 claims abstract description 13
- 239000003814 drug Substances 0.000 claims abstract description 13
- 206010065687 Bone loss Diseases 0.000 claims abstract description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 9
- 238000002360 preparation method Methods 0.000 claims abstract description 7
- 239000002904 solvent Substances 0.000 claims abstract description 6
- 239000003960 organic solvent Substances 0.000 claims abstract description 5
- 239000012153 distilled water Substances 0.000 claims abstract description 4
- 238000010992 reflux Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 241000628997 Flos Species 0.000 claims description 35
- 230000006837 decompression Effects 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- 230000002265 prevention Effects 0.000 claims description 8
- 239000000469 ethanolic extract Substances 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- 238000004064 recycling Methods 0.000 claims description 6
- 230000001476 alcoholic effect Effects 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 3
- 206010039073 rheumatoid arthritis Diseases 0.000 claims description 3
- 150000001335 aliphatic alkanes Chemical class 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 125000004432 carbon atom Chemical group C* 0.000 claims description 2
- 150000002148 esters Chemical class 0.000 claims description 2
- 238000000638 solvent extraction Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 9
- 229940079593 drug Drugs 0.000 abstract description 5
- 235000003255 Carthamus tinctorius Nutrition 0.000 abstract 1
- 244000020518 Carthamus tinctorius Species 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 230000000452 restraining effect Effects 0.000 abstract 1
- 208000006386 Bone Resorption Diseases 0.000 description 6
- 230000024279 bone resorption Effects 0.000 description 6
- 208000035896 Twin-reversed arterial perfusion sequence Diseases 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 102000014128 RANK Ligand Human genes 0.000 description 3
- 108010025832 RANK Ligand Proteins 0.000 description 3
- 230000017531 blood circulation Effects 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- 201000000736 Amenorrhea Diseases 0.000 description 2
- 206010001928 Amenorrhoea Diseases 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 208000005171 Dysmenorrhea Diseases 0.000 description 2
- 206010013935 Dysmenorrhoea Diseases 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 231100000540 amenorrhea Toxicity 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000011164 ossification Effects 0.000 description 2
- 230000003119 painkilling effect Effects 0.000 description 2
- 230000008736 traumatic injury Effects 0.000 description 2
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 206010029240 Neuritis Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 102000007591 Tartrate-Resistant Acid Phosphatase Human genes 0.000 description 1
- 108010032050 Tartrate-Resistant Acid Phosphatase Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 208000024756 faint Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002175 menstrual effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 201000001119 neuropathy Diseases 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 206010042772 syncope Diseases 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a safflower extract applied to medicines for preventing and treating bone loss diseases. The extract has excellent effect of restraining the formation of osteoclast, and can be used for preparing the medicines for treating bone loss diseases such as osteoporosis. The preparation method of the safflower extract comprises the steps of using ethanol for reflux extraction on dried safflowers; recovering the ethanol to obtain extract; dissolving the extract in distilled water; extracting by using an organic solvent; dissolving the extract in ethanol; recovering the solvent to obtain the safflower extract. The safflower extract is from natural medicines, so that the side effects are few, and the use is safe.
Description
Technical field
The present invention relates to Flos Carthami extract, especially relate to the application of Flos Carthami extract in the medicine of preparation prevention and treatment bone loss disease.
Background technology
Along with world population aging becomes clear day by day, the sickness rate of osteoporosis raises year by year, has directly been affected the life quality of middle-aged and elderly people by the osteodynia due to osteoporosis and fracture, has become severe social problem.Human skeleton, by continuous osteogenesis and bone resorption, is being transformed always, and this transformation is transformed in bone.Keeping good balance in osteogenesis at an early age and bone resorption, but with advancing age, because this balance of a variety of causes is tended to bone resorption, the words that continue for a long time this state can make osseous tissue become fragile, and cause occurring the various osteopathias such as osteoporosis, fracture.In human body, osteoclast is the unique cell that has bone resorption.Research shows, when osteoclast formation becomes Showed Very Brisk, will cause the various osteopathias such as the above-mentioned osteoporosis (osteoporosis) causing due to bone loss.Therefore, formation and the bone resorption function of inhibition osteoclast are a kind of methods of very effective treatment osteoporosis.
Developed at present the prevention of multiple inhibition osteoclast formation and medicine and the method for the treatment of osteoporosis, but high owing to having price, long-term taking causes multiple side effect, thereby has certain limitation on using.Compared with synthetic new drug, natural medicinal ingredients has safety, inexpensive advantage.
Summary of the invention
The object of the present invention is to provide a kind of natural drug that derives from, there is the plant extract in the medicine that suppresses osteoclast formation, is applied to preparation prevention and treatment bone loss disease.
Suppressing osteoclast is the effective way of the bone loss diseases such as treatment osteoporosis.Applicant of the present invention finds that the extract of Flos Carthami has the effect of good inhibition osteoclast formation, can be applied to the medicine of preparation prevention and treatment bone loss disease.
The invention provides the application of Flos Carthami extract in the medicine of preparation prevention and treatment bone loss disease.
The present invention further provides the application of Flos Carthami extract in the medicine of preparing prevention and treatment osteoporosis, rheumatoid arthritis.
Described Flos Carthami extract has the effect of good inhibition osteoclast formation.
Described in also providing, the present invention there is the Flos Carthami extract extracting method that suppresses osteoclast formation:
1. get dry Flos Carthami, use alcohol reflux 1-3 time, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, obtains ethanol extract;
2. ethanol extract is added to distilled water thermosol, then use organic solvent extraction, decompression and solvent recovery, obtains extractum I;
3. the dissolve with ethanol of extractum I doubly being measured with 2-10, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, must suppress the Flos Carthami extract of osteoclast formation.
In preferred extracting method, at the step alcoholic solution that 1. described ethanol is 60-80%.
In preferred extracting method, step 2. described organic solvent be the alcohol that contains 1-6 carbon atom, alkane or esters.
In preferred extracting method, at the step alcoholic solution that 3. described ethanol is 30-100%.
Flos Carthami, for conventional Chinese herbal medicine, is mainly used in amenorrhea, dysmenorrhea, lochia, lump in the abdomen mass in the abdomen, traumatic injury.When ancients often have what blood in vivo, often add Flos Carthami tate: bundle is boiled available one day twice foot bath, be suitable for various varicosis, peripheral neuritis, blood circulation.Invigorating blood circulation can be through; Stasis-dispelling and pain-killing, Flos Carthami acrid in the mouth, warm in nature, GUIXIN, Liver Channel.The fragrant row of gas is loose, enters blood system and has promoting blood circulation to restore menstrual flow, the effect dysmenorrhea of stasis-dispelling and pain-killing, amenorrhea, puerperal blood faints, the stagnant stomachache of the stasis of blood, obstruction of qi in the chest and cardialgia, accumulation of blood, traumatic injury congestive edema, arthralgia, paralysis due to windstroke, macular eruption being dark-purple.Though Flos Carthami has above-mentioned multiple efficacies, and coordinate the treatment of other Chinese herbal medicine for osteoporosis, still do not have Flos Carthami extract to suppress the report of osteoclast formation.
Flos Carthami extract of the present invention has the effect of good inhibition osteoclast formation, and source and natural drug, and few side effects has the safe effect that uses.
Flos Carthami extract of the present invention, extracting method is simple, is easy to suitability for industrialized production.
The specific embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment 1
1. get dry Flos Carthami, with 3 times of 70% alcohol reflux 2 times, each 2 hours, merge extractive liquid,, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, obtains ethanol extract;
2. the distilled water thermosol that ethanol extract is added to 5 times of amounts, is then extracted with ethyl acetate, and decompression and solvent recovery obtains extractum I;
3. 80% dissolve with ethanol by 5 times of amounts by extractum I, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, must suppress the Flos Carthami extract of osteoclast formation.
Extract is added to pharmaceutical carrier, mix, granulate, dry, obtain granule.This granule can be used for the treatment of bone loss disease, as osteoporosis, and rheumatoid arthritis etc.
Embodiment 2
The Flos Carthami extract that adopts embodiment 1 to obtain, measures its impact on osteoclast formation.
The formation system of osteoclast: adopt RAW264.7 cell under RANKL exists, cultivate the osteoclast that just can be formed as multinuclear for 3-5 days in α-MEM culture fluid.Tartrate resistant acid phosphatase for osteoclast (TRAP) staining forming is observed the positive apocyte of TRAP, and several 3 positive apocytes of above nuclear TRAP under the microscope, as the index of osteoclast formation.
Described RAW264.7 is the strain of cell mouse Monocytes/Macrophages, can be formed as the osteoclast of bone resorption ability under the existence of the osteoclast formation factor (RANKL).
Flos Carthami extract is dissolved in α-MEM culture fluid, be mixed with finite concentration, then join in the osteoclast formation system of RAW264.7 cell, in the cell culture incubator of 5% carbon dioxide and 37 ℃, cultivate 3 days, the osteoclast obtaining is sent out dyeing with TRAP dyeing, and statistics has 3 positive osteoclasts of above nuclear TRAP under the microscope, the results are shown in Table 1.
The impact of table 1. Flos Carthami extract on osteoclast formation
In the test of table 1: RAW264.7 cell is with 5 × 10
3the concentration of/well is inoculated in 96 orifice plates, in the α-MEM culture fluid that is 10ng/ml, cultivates 3 in RANKL concentration.
In table, the concentration of Flos Carthami extract is the ultimate density in cell culture fluid, and each concentration is established 3 holes, and osteoclast number is the average in 3 holes.
In table, matched group is the solvent using in the time that Flos Carthami extract concentration is 10ug/ml, as solvent control.
In table, suppression ratio is the suppression ratio of Flos Carthami extract to osteoclast formation calculating with respect to matched group.
The result of table 1 is known, and Flos Carthami extract significantly suppresses the formation of osteoclast, along with its suppression ratio of increase of concentration also increases.
Claims (6)
1. the Flos Carthami extract application in the medicine of preparation prevention and treatment bone loss disease.
2. the Flos Carthami extract application in the medicine of preparation prevention and treatment osteoporosis, rheumatoid arthritis.
3. according to the application of the Flos Carthami extract described in claim 1 and 2, it is characterized in that, described Flos Carthami extract extracts and obtains with the following method:
1. get dry Flos Carthami, use alcohol reflux 1-3 time, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, obtains ethanol extract;
2. ethanol extract is added to distilled water thermosol, then use organic solvent extraction, decompression and solvent recovery, obtains extractum I;
3. the dissolve with ethanol of extractum I doubly being measured with 2-10, cold preservation is spent the night, and filters, and decompression filtrate recycling ethanol, must suppress the Flos Carthami extract of osteoclast formation.
4. the application of Flos Carthami extract according to claim 3, is characterized in that: at the step alcoholic solution that 1. described ethanol is 60-80%.
5. the application of Flos Carthami extract according to claim 3, is characterized in that: step 2. described organic solvent be the alcohol that contains 1-6 carbon atom, alkane or esters.
6. the application of Flos Carthami extract according to claim 3, is characterized in that: at the step alcoholic solution that 3. described ethanol is 30-100%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210469067.0A CN103816198A (en) | 2012-11-16 | 2012-11-16 | Safflower extract and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210469067.0A CN103816198A (en) | 2012-11-16 | 2012-11-16 | Safflower extract and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103816198A true CN103816198A (en) | 2014-05-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210469067.0A Pending CN103816198A (en) | 2012-11-16 | 2012-11-16 | Safflower extract and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103816198A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107343947A (en) * | 2017-07-25 | 2017-11-14 | 徐冰娜 | A kind of preparation method of the clear ball bacteriostatic agent of woman |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1709274A (en) * | 2005-06-08 | 2005-12-21 | 广东医学院 | New application of pharmaceutical preparations with improved brain metabolism to prevent and treat osteoporosis |
| WO2008004118A2 (en) * | 2006-07-06 | 2008-01-10 | Avestha Gengraine Technologies Pvt. Ltd. | Carthamus tinctoris plant extracts for treating osteoporosis and the extraction process thereof |
-
2012
- 2012-11-16 CN CN201210469067.0A patent/CN103816198A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1709274A (en) * | 2005-06-08 | 2005-12-21 | 广东医学院 | New application of pharmaceutical preparations with improved brain metabolism to prevent and treat osteoporosis |
| WO2008004118A2 (en) * | 2006-07-06 | 2008-01-10 | Avestha Gengraine Technologies Pvt. Ltd. | Carthamus tinctoris plant extracts for treating osteoporosis and the extraction process thereof |
Non-Patent Citations (2)
| Title |
|---|
| EUN MI CHOI等: "Carthamus tinctorius Flower Extract Prevents H2O2-Induced Dysfunction and Oxidative Damage in Osteoblastic MC3T3-E1 Cells", 《PHYTOTHERAPY RESEARCH》 * |
| 刘钰瑜等: "红花水提液对老年去卵巢大鼠骨生物力学状况的改善作用", 《中国老年学杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107343947A (en) * | 2017-07-25 | 2017-11-14 | 徐冰娜 | A kind of preparation method of the clear ball bacteriostatic agent of woman |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140528 |
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| WD01 | Invention patent application deemed withdrawn after publication |