CN103301256A - Preparation method of medicinal plant extract being capable of reducing blood fat and application thereof in pharmacy and health food - Google Patents

Abstract

The invention relate to a preparation method of a medicinal plant extract being capable of reducing blood fat and an application thereof in pharmacy and health food. The extract is prepared by using a medicinal plant-Pandanus tectorius in south of the Five Ridges as a raw material, extracting and separating. The preparation method comprises extracting the Pandanus tectorius by water and/or alcohol, and then condensing and drying the extract to obtain the medicinal plant extract provided by the invention. The extract is dissolved in water, degreased, and extracted by an organic solvent to obtain an extraction part; or passes through macroporous adsorption resin, and then elute by water, methanol, ethanol, acetone or 60-90 % ethanol or aqueous acetone; the extract liquor or the eluent is collected, recovered under reduced pressure, condensed into extract, and then dried to obtain effective parts of the medicinal plant extract. A plurality of pharmacodynamics experiments verify that the medicinal plant extract is capable of reducing the blood fat, can be used for preparing drugs or health products of reducing the blood fat, and thus is used for treating hyperlipidemia, obesity and other diseases.

Description

Preparation method of medicinal plant extract with hypolipidemic properties and its application in pharmacy and health food

technical field

The invention relates to the field of lipid-lowering traditional Chinese medicine and health food, in particular to a preparation method of a lipid-lowering and weight-loss medicinal plant-wild pineapple extract and its effective part and its application in the development of pharmacy and health care products. the

Background technique

Hyperlipidemia is one of the main causes of cardiovascular and cerebrovascular diseases and a potential factor that threatens human health. At present, the use of lipid-lowering drugs is the most favorable measure for the prevention and treatment of hyperlipidemia. Compound preparations or Chinese patent medicines currently available on the market include Jiangzhining, Xuezhikang, Dicikang, Diao Xinxuekang, etc. The research and development of blood lipid-lowering traditional Chinese medicine and ethnic medicine has also been in the ascendant, such as hawthorn, Jiaogulan, ginseng, rhubarb, Panax notoginseng, cassia seed, wolfberry and so on. In the process of these studies, it was found that many have significant blood lipid-lowering activities such as saponins, flavonoids, polysaccharides, etc. At the same time, a lot of research has been carried out on the blood-lipid-lowering mechanism of these active ingredients, including inhibiting the absorption of cholesterol, inhibiting HMG-CoA Factors such as enzymes, elevated SOD, and NO achieve the effect of lowering blood lipids, which undoubtedly laid a scientific foundation for the subsequent research and development of blood lipid-lowering medicinal plants or ethnic medicines. the

Wild pineapple is the fruit of Pandanus tectorius Soland. in the family Pandanaceae. Wild pineapple, which grows in tropical or subtropical regions, is distributed in Guangdong, Guangxi, Yunnan, Hainan, Taiwan and other places in China. Nanning Drug Records. Wild pineapple has a strange shape, is hard and contains wood, and it is recorded in literature that it can nourish the spleen and stomach, strengthen vitality, relieve alcohol poisoning, and treat mainly liver heat deficiency fire, liver cirrhosis and ascites. the

The beneficial effects and significance of the present invention are: the extract and the effective part in the present invention are obtained by extracting and purifying the fruit of a medicinal plant of the genus Pandanus, Landaus tectorius Soland., which is very rich in plant resources , our study found that it can be used as an effective drug or health care product for lipid-lowering and weight-loss. At the same time, it has very important practical significance for the further comprehensive utilization of this medicinal plant and the development of the Lingnan medicinal material economy. the

Contents of the invention

The invention provides the preparation of the medicinal plant extract and its application in the development of lipid-lowering drugs and health care products. the

The preparation method of lipid-lowering medicine or health product extract of the present invention comprises the following steps:

(1), extraction:

Take wild pineapple, clean it, break it into pieces, add it to 10-20 times of water or/and alcohol, soak overnight, decoct, and filter; add water or/and alcohol to extract the filtered medicinal residue for 1-3 times, filter, Combine the filtrates, then concentrate the filtrate under reduced pressure, heat to 60°C, the concentrated solution has a relative density of 1.10-1.30 according to thermal measurement, and cool the concentrated solution to room temperature. the

(2), purification:

Add the concentrated medicinal solution to neutral water at a ratio of crude drug weight:water volume ratio of 0.5-3:1, heat to 30-50°C, stir to dissolve, let stand for 2-24 hours, and filter to obtain the filtrate;

After the obtained filtrate is degreased with an organic solvent, it is extracted or extracted with an organic solvent, and the obtained extract or extract is concentrated and dried to obtain the effective part, or the obtained extract is adsorbed on a macroporous adsorption resin, and firstly use 3 Wash with water of ~5 column bed volumes to remove impurities, and then elute with an eluting solvent until no obvious spots are detected by thin-layer chromatography; collect the eluate and dry it to obtain the effective part;

The organic solvent used for degreasing is sherwood oil, benzene, chloroform, ethyl acetate, cyclohexane, n-hexane. the

The extraction or extraction solvent used is acetone, ethanol, methanol, n-butanol, propanol, isopropanol, ethyl acetate, water, ethanol-water solution, methanol-water solution. the

The elution solvent is water, methanol, ethanol, acetone, or 50-95v% ethanol or acetone aqueous solution. the

The macroporous resins used include D series, HPD series, HP series, XAD series and AB-8 macroporous adsorption resin. the

The preparation method of the medicinal plant extract and effective part of the present invention comprises mixing the medicinal plant extract or effective part of the present invention with a pharmaceutically acceptable carrier. the

The medicinal plant extract of the present invention can be made into various dosage forms, such as powder, tablet, pill, dispersion, capsule, soft capsule, drop pill, granule, effervescent tablet, tea bag, oral liquid , Health drinks, etc. the

According to the technical contents disclosed in the present invention, other embodiments of the present invention will be apparent to those skilled in the art, and the following embodiments are only examples. Various changes and improvements can be made to the present invention without departing from the spirit and scope of the present invention. These changes and improvements should all fall within the protection scope of the present invention. the

Description of drawings

Figure 1: The results of the blood lipid-lowering effect of wild pineapple fruit water extract

Figure 2: The blood lipid-lowering efficacy of the effective parts of medicinal plants of the present invention

Detailed ways

The present invention will be further described in detail below in conjunction with examples of implementation. the

Example 1 Preparation of medicinal plant extracts and effective parts thereof in the present invention (one)

Take fresh and ripe wild pineapple, put it in a container, add 10-20 times of water, soak for 2 hours, decoct 3 times for 2 hours each time, filter, concentrate until dry to obtain the medicinal plant extract. After the medicinal plant extract is dissolved in water, it is separated by D-101 macroporous adsorption resin, eluted with different concentrations of ethanol-water solution until it is colorless, and the eluate is concentrated under reduced pressure to obtain the water eluate, 70% The ethanol eluate is concentrated and spray-dried to obtain the effective fraction of the present invention. the

Example 2 Preparation of medicinal plant extracts and effective parts thereof in the present invention (two)

Take fresh, mature and shade-dried wild pineapple, put it in a container, add 10-20 times of ethanol or ethanol-water (7:3), soak for 2 hours, extract 3 times in the same flow, 2 hours each time, filter, and concentrate until dry. Medicinal plant extracts. After the medicinal plant extract is dissolved in water, degreased by the organic solvent chloroform or ethyl acetate, it is repeatedly extracted with n-butanol until it is colorless, the extract is concentrated under reduced pressure, and spray-dried to obtain the effective part of the present invention . the

Embodiment 3 Pharmacological research on hypolipidemic effect of medicinal plant extracts of the present invention

Test materials: wild pineapple water extract, Zhimaikang, high-fat feed, kit (total cholesterol)

Test animals: healthy Kunming mice (18-20g)

Test method: 60 male Kunming mice of 18-22g were randomly divided into normal group, hyperlipidemia model group and 5 dosage groups of wild pineapple water extract (doses were 25g/kg, 50g/kg, 100g/kg respectively) , 4.0mg/kg simvastatin group, 20g/kg Zhimaikang group, a total of 9 groups (10 rats/group), except for the normal group fed with conventional feed, the other groups were fed with high-fat feed, and at the same time given the corresponding drugs by intragastric administration, The normal group and the hyperlipidemia model group were intragastrically administered with the same volume of tap water. After 18 days of administration, the mice in each group were fasted and watered for 12 hours, the eyeballs were removed to collect blood, centrifuged at 3000r/min for 10 minutes, and the serum was collected, and the serum TC content was measured by ultraviolet spectrophotometry with a kit. the

Experimental results: The blood lipid-lowering activity of wild pineapple fruit water extract was studied with a hyperlipidemic mouse model, and Simvastatin and Zhimaikang were used as positive control drugs. The activity of total cholesterol (TC) in rat plasma is significant, and the activity of lowering plasma total cholesterol at a low concentration of 25g/Kg is already very significant (p<0.01), and the effect is obviously better than that of the traditional Chinese medicine Zhimaikang. the

N＝10, vs control, ###P＜0.01; vs model*P＜0.05, **P＜0.01. 

Embodiment 4 The lipid-lowering activity test of effective part of medicinal plant of the present invention (golden hamster hyperlipidemia model):

Test materials: effective part, berberine, high-fat feed, kit including total cholesterol (TC), total triglyceride (TG), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C) . the

Experimental animal: healthy golden hamster (130-160g)

Test method: Take 60 golden hamsters of 130～160g, and randomly divide them into 1 dosage group of wild pineapple water extract, 3 dosage groups of high, middle and low effective parts, 1 group of positive drug, model group, and blank group, totally 7 groups (8 rats) /group), except for the normal group fed with conventional feed, other groups were fed with high-fat feed (2% cholesterol, 15% lard, 0.4% bile salt, 82.6% basal feed), and the corresponding drugs were given by intragastric administration at the same time, the normal group and The hyperlipidemia model group was given an equal volume of tap water orally. After 21 days of administration, the golden hamsters in each group were fasted and watered for 12 hours, their eyeballs were removed to take blood, centrifuged at 3000r/min for 10min, and serum was taken, and serum TC, TG, LDL-C, HDL- C content. the

N＝8, vs control, ###P＜0.01; vs model *P＜0.05, **P＜0.01, ***P＜0.001. 

Note: the dose of berberine group is 200mg/Kg, the water extract 400mg/Kg, the high dose of effective parts is 400mg/kg, the middle dose group of effective parts is 200mg/Kg, and the low dose group of effective parts is 100mg/Kg. the

Example 5 Preparation of medicinal plant extract or effective part powder in the present invention

Grind the medicinal plant extracts or effective parts and effective parts prepared in the examples into fine powder and sieve, take 1 part of the sifted medicinal plant extract fine powder, add 3.5 parts of starch and 6.5 parts of lactose Mix evenly by equal volume incremental mixing method, and make powder according to the volumetric method. the

Embodiment 6 The preparation example of medicinal plant extract or effective part hard capsule of the present invention

Crush the medicinal plant extract or effective part obtained in Example 1 or 2, pass through a 60-100 mesh sieve, take 1 part of the sifted medicinal plant extract fine powder, add 1.1 parts of dry starch as filling agent, add 1-3% talcum powder as a glidant, mix and fill with empty capsules to prepare hard capsules. the

Example 7 Preparation of medicinal plant extract or effective part tablet in the present invention

Grind the medicinal plant extract or effective part obtained in Example 1 or 2 through a 60-100 mesh sieve, take 1 part of the sieved medicinal plant extract fine powder, add 1.1 parts of hydroxypropyl cellulose As a disintegrant, add 0.4 parts of medicinal dextrin as a binder, add an appropriate amount of 50-95% ethanol as a lubricant, wet granulate, dry, and granulate, add 1-3% lubricant, mix evenly, and press into tablets. the

Example 8 Preparation of Medicinal Plant Extract or Effective Fraction Dispersible Tablets in the present invention

Dissolve 1 part of the medicinal plant extract or effective part and 4 parts of the carrier material in 50-95% ethanol prepared in Example 1 or 2, dry by spray drying, sieve, and add 2.0 parts of Microcrystalline cellulose is used as a disintegrant, and an appropriate amount of micropowdered silica gel is added as a glidant. After mixing evenly, the powder is directly compressed into tablets to obtain dispersible tablets. the

Embodiment 9 Preparation example of medicinal plant extract or effective part health drink in the present invention

Dissolve one part of medicinal plant extract or effective part and 0.001 part of sugar-free sweetener in food-grade water prepared in Example 1 or 2, add flavoring agents to prepare beverages of various tastes, and immediately after filling Get a health drink. the

Claims (14)

1. A medicinal plant extract and effective parts thereof for lowering blood fat, characterized in that: taking wild pineapple (Pandoucarpa fruit) as a raw material through extraction and concentration, and the effective part is that the medicinal plant extract is processed by an organic solvent. Extracted or extracted or purified by macroporous adsorption resin. 2. according to the preparation method of medicinal plant extract described in claim 1 and effective part thereof, it is characterized in that the method contains following ①, ②, ③ three steps: ①: Take wild pineapple, add 10 to 30 times the amount of water or/and alcohol to extract 2 to 4 times, each time for 1 to 3 hours, combine the extracts, filter, and concentrate under reduced pressure. After the filtrate is concentrated and dried, this product is obtained. The medicinal plant extract in the invention; ②: Effective part extraction process I: Take the extract of ①, dissolve it in water to obtain a certain concentration of aqueous solution, add it to the filled macroporous adsorption resin column, and rinse the resin column with distilled water until the washing process is completed. The eluate is colorless; then add an eluent to the resin column, collect the eluate, concentrate and dry, and finally obtain the effective fraction of the present invention. ③: Effective part extraction process II: take the extract in ①, dissolve it in water to obtain an aqueous solution of a certain concentration, after degreasing, add a solvent for extraction or extraction, and after the extract or the extract is concentrated and dried, the extract of the present invention is obtained. effective part. 3. according to the preparation method described in claim 2 1., it is characterized in that: used extraction solvent is water or/and alcohol. 4. The preparation method according to claim 2①, characterized in that: decocting or reflux extraction is used, and the number of extractions is 2 to 4 times, and each time is 1 to 3 hours. 5. According to the preparation method described in claim 2②, it is characterized in that: the chromatographic column packing used comprises D series, HPD series, HP series, XAD series and AB-8 macroporous adsorption resin. 6. According to the preparation method described in claim 2②, it is characterized in that: the eluent used is water, methanol, ethanol, acetone, or an aqueous solution of 50-95v% ethanol or acetone. 7. according to the degreasing method described in claim 23., it is characterized in that: the organic solvent for degreasing is sherwood oil, benzene, chloroform, ethyl acetate, hexanaphthene, normal hexane, gasoline etc. 8. According to the extraction or extraction method described in claim 23., it is characterized in that: extraction or extraction organic solvent is acetone, ethanol, methyl alcohol, n-butanol, propanol, Virahol, ethyl acetate, water, ethanol- Aqueous solution, methanol-water, acetone-water solution. 9. The medicinal plant extract and its effective parts according to claim 1, characterized in that: the medicinal plant extract and its effective parts have hypolipidemic effect. 10. The application of a kind of medicinal plant extract and its effective parts described in claim 1 in the preparation of blood lipid-lowering drugs. 11. The application of a kind of medicinal plant extract and its effective parts described in claim 1 in the preparation of blood lipid-lowering and weight-loss health care products. 12. The medicinal plant extract and its effective part preparation according to claim 1, characterized in that: it also includes conventional pharmaceutical excipients. 13. The lipid-lowering and weight-reducing medicine according to claim 10, characterized in that: it includes powder, tablet, pill, dispersion, capsule, soft capsule, drop pill, granule, effervescent tablet, tea bag, oral liquid , health drinks and so on. 14. The health product for reducing fat and losing weight according to claim 8, characterized in that it includes beverages, capsules, effervescent tablets, teabags, oral liquids, granules and the like.

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