CN103196815A - Urinary sediment counting chamber - Google Patents

Abstract

The invention discloses a urinary sediment counting chamber which comprises a base, wherein a bottom solid phase supporting structure, an isolation slide and a fixing slide are arranged on the base from bottom to top; an opening is formed in the isolation slide; the bottom solid phase supporting structure consists of a lower transparent silica gel plate and a silica gel pressing sheet arranged on the lower transparent silica gel plate; an opening is formed in the silica gel pressing sheet; the base, the transparent silica gel plate, the silica gel pressing sheet, the isolation slide and the fixing slide are stuck together to form a cavity serving as a detection inner cavity; channels for enabling sample liquid to be in and out of the detection inner cavity are formed on the transparent silica gel plate and the silica gel pressing sheet; a sample inlet hole and a sample outlet hole which are communicated with the channels are respectively formed in the base and are respectively connected with a sample inlet pipe and a sample outlet pipe; a working channel is arranged on the base; and as force is applied by the working channel, the bottom solid phase supporting structure can do reciprocating motion in the detection inner cavity. The counting chamber can quickly fix visible components such as cells in the sample liquid on a plane, so that a user can observe the visible components with a microscope conveniently.

Description

urine sediment counting tank

technical field

The invention relates to the technical field of urine microscope and blood microscope detection, in particular to a counting pool of a urine sediment analysis system.

Background technique

In the existing urine sediment analysis system that adopts flow microscopy, the structure of the counting cell used is shown in Figure 1 (for a double-channel counting cell), including a base, a partition glass arranged on the base, and a The upper slide (i.e. the fixed slide) on the septum slide, wherein the base and the upper slide are fixed, and an opening is opened in the center of the septum slide, and the base, the septum slide, and the upper slide are bonded together. Together, they form a cavity, which is the detection inner cavity; holes communicating with the detection inner cavity are opened on the base, and these holes are respectively connected with the sample inlet tube and the sample outlet tube. The detection process is as follows: the sample liquid enters the detection cavity through the sampling tube, and then stands still, and the formed components such as cells settle to the lower glass slide by the action of gravity, so that the formed components such as cells have the same focal plane of the microscope objective lens (As shown in Figure 2), the system then scans and detects the cells on the lower slide to obtain a clear image. Although a clear image can be obtained by using the above-mentioned structure counting cell for detection and counting, since the cells and other formed components in the sample liquid are naturally settled by gravity, it takes 2 to 5 minutes to make the cells, crystals, and tubes in the sample liquid settle down. It takes a long time for the formed components such as type to completely settle on the base.

Contents of the invention

The technical problem to be solved by the present invention is to provide a urine sediment counting pool that can quickly settle cells and other formed components in the sample liquid onto the detection surface and obtain clear images.

The urine sediment counting tank according to the present invention comprises a base on which a bottom solid-phase support structure, a partition glass and a fixed slide are arranged from bottom to top, and an opening is opened in the center of the partition glass, wherein:

The bottom solid-phase support structure is composed of a transparent silica gel plate placed on the lower layer, and a silica gel pressing sheet placed on it. There is an opening on the silica gel pressing sheet. After the base, the transparent silica gel plate, and the silica gel pressing sheet are placed After bonding together, the spacer slide, the spacer slide and the fixed slide form a cavity, which is the detection inner cavity; there are passages for the sample liquid to enter and exit the detection inner cavity on the silica gel sheet and the transparent silica gel plate. A sample inlet and a sample outlet are provided on the top to communicate with the passage of the above-mentioned sample supply liquid entering and exiting the detection inner cavity, and the sample inlet and sample outlet are respectively connected to the sample inlet and the sample outlet;

An action channel communicating with the bottom part of the bottom solid-phase support structure is also provided on the base, and force is applied through the action channel to make the bottom solid-phase support structure reciprocate in the detection inner cavity.

The present invention adopts a special bottom solid-phase support structure design, and at the same time, an action channel communicating with the bottom part (transparent silica gel plate) of the bottom solid-phase support structure is set on the base, combined with the transparent silica gel plate, which is transparent, soft, and has certain elasticity By applying force to the action channel, the bottom solid-phase support structure can reciprocate up and down in the detection cavity. When a positive pressure is applied to the transparent silica gel plate through the action channel, the transparent silica gel plate moves away from the base, and finally closes to the fixed glass slide, so that the formed components such as cells in the sample liquid are fixed on the transparent silica gel plate, Then, the formed components such as cells in the sample liquid settle on a plane for the purpose of microscope observation; when negative pressure is applied to the transparent silica gel plate through the action channel, the transparent silica gel plate is away from the fixed slide and returns to the surface of the base , at this time to achieve the purpose of convenient cleaning. Since the transparent silica gel plate is pushed to the fixed glass slide so that the formed elements such as cells in the sample liquid are fixed on the transparent silica gel plate, therefore, compared with the natural sedimentation method in the prior art, the formed elements such as fixed cells The location is faster.

In the above technical solution, the bottom solid-phase support structure can also be: composed of a transparent silica gel plate placed on the lower layer, a silica gel sheet placed on the transparent silica gel plate, and a moving glass placed between the two, There is an opening on the silica gel pressing sheet, and the moving glass is located within the range of the orthographic projection of the opening on the silica gel pressing sheet and facing the action channel; when the base, transparent silica gel plate, moving glass, and silica gel pressing After bonding together, the spacer slide, the spacer slide and the fixed slide form a cavity, which is the detection inner cavity; there are passages for the sample liquid to enter and exit the detection inner cavity on the silica gel sheet and the transparent silica gel plate. A sample inlet and a sample outlet are opened on the top to communicate with the channels for the above-mentioned sample liquid to enter and exit the detection inner chamber, and the sample inlet and sample outlet are respectively connected to the sample inlet and the sample outlet. The bottom solid-phase support structure adds a moving glass between the transparent silica gel plate and the silica gel pressing plate. When a positive pressure is applied to the transparent silica gel plate through the action channel, as the transparent silica gel plate and the moving glass move, the Formed components such as cells in the sample solution are immobilized on a transparent silica gel plate.

In the above technical solution, the bottom solid-phase support structure may also be: composed of a moving glass, a partition, a transparent silica gel plate, and a silica gel sheet arranged sequentially from bottom to top, and the silica gel sheet and the partition There are openings on the top, and the moving glass is located within the range of the orthographic projection of the opening on the silica gel sheet and the partition and is facing the action channel; when the base, the partition, the moving glass, and the transparent silica gel plate , Silicone pressing sheet, spacer slide and fixed slide are bonded together to form a cavity, which is the detection inner cavity; there are channels for the sample liquid to enter and exit the detection inner cavity on the silicone pressing sheet and transparent silica gel plate. A sample inlet and a sample outlet are opened on the base to communicate with the channels for the sample liquid to enter and exit the detection inner cavity, and the sample inlet and sample outlet are respectively connected to the sample inlet and the sample outlet. The bottom solid-phase support structure is provided with a partition with an opening on the lower layer of the transparent silica gel plate and the silica gel pressing sheet, and a moving glass is arranged within the range of the orthographic projection of the opening on the partition. When the pressure is applied, the opening on the partition reserves a space for the moving glass to reciprocate up and down. With the movement of the moving glass, the transparent silica gel plate is pushed to the fixed glass, so that the formed components such as cells in the sample liquid Fixed on a transparent silicone plate.

In the solid-phase support structure at the bottom of the above technical solution, a plastic film (specifically, a polyvinyl chloride plastic film) can also be used to replace the transparent silica gel plate, and the effect is the same.

In the above technical solution, the function channel is a channel opened on the base and communicated with the bottom part of the bottom solid phase support structure. It can be a through hole of other shapes such as circular or square. The purpose of the function channel It is to connect with a system that can provide positive pressure or make negative pressure in the detection cavity through pipelines. Specifically, the equipment that can provide positive pressure can be a diaphragm pump or a plunger pump, etc., and can make the negative pressure in the detection cavity The equipment can be diaphragm pump or plunger pump etc. When positive pressure is provided into the detection inner cavity, its magnitude is sufficient as long as it can realize the movement of the lowermost part of the bottom solid phase support structure towards the fixed glass slide. The applicant found through experiments that when a pressure of 2 to 3 atmospheres is provided in the action channel, it is better in terms of speed and effect. At this time, the lowest part of the solid phase support structure at the bottom is pushed to make the liquid in the sample liquid It takes about 3 seconds for cells and other formed components to be immobilized on the transparent silica gel plate.

Compared with the prior art, the present invention adopts a special bottom solid-phase support structure design, and at the same time, an action channel communicating with the bottom part of the bottom solid-phase support structure is set on the base, combined with a transparent silica gel plate that is transparent, soft, and has a certain With the characteristics of elasticity, the force is applied to the bottom part of the bottom solid phase support structure through the action channel, so that the bottom part of the bottom solid phase support structure can reciprocate up and down in the detection cavity, so as to realize the rapid removal of the sample liquid. Formed components such as cells are fixed on a plane for the purpose of microscope observation, and finally a clear image is obtained.

Description of drawings

Fig. 1 is the structural representation of counting pool in the prior art;

Fig. 2 is a schematic diagram of the structure of cells and other formed components in the sample liquid in Fig. 1 when they settle on the base for microscope observation;

Fig. 3 is a schematic structural view of an embodiment of the present invention;

Fig. 4 is the front view of the embodiment shown in Fig. 3;

Fig. 5 is the enlarged schematic view of I place in Fig. 4;

Fig. 6 is a schematic structural view of cells and other formed components in the sample liquid in the embodiment shown in Fig. 3 when they settle on a transparent silica gel plate for microscope observation;

Fig. 7 is a schematic structural view of another embodiment of the present invention;

Figure 8 is a front view of the embodiment shown in Figure 7;

Fig. 9 is an enlarged schematic view at S in Fig. 8;

Fig. 10 is a schematic diagram of the structure of cells and other formed components in the sample liquid in the embodiment shown in Fig. 7 when they are deposited on a moving glass slide for microscope observation;

Fig. 11 is a schematic structural diagram of yet another embodiment of the present invention;

Fig. 12 is a front view of the embodiment shown in Fig. 11;

Fig. 13 is an enlarged schematic view of H in Fig. 12;

FIG. 14 is a structural schematic view of cells and other formed components in the sample solution in the embodiment shown in FIG. 11 when they settle on a transparent silica gel plate for microscope observation.

The labels in the figure are:

1 fixed slide; 2 partition slide; 3 base; 4 sample tube; 5 sample tube; 6 microscope; 7 light source; 8 silica gel sheet; 9 transparent silica gel plate; 10 pipe; sheet; 13 partitions.

Detailed ways

3-6 are diagrammatic illustrations of a first embodiment of the present invention. Referring to Figures 3 to 6, the urine sediment counting tank according to the present invention includes a base 3 on which a bottom solid-phase support structure, a spacer slide 2 and a fixed slide 1 are arranged from bottom to top on the base 3, and the spacer slide 2 There is an opening in the center, of which:

The bottom solid-phase support structure is made up of a transparent silica gel plate 9 placed on the lower floor, and a silica gel pressing sheet 8 placed on the transparent silica gel sheet 9, and an opening is provided on the silica gel pressing sheet 8 (the opening is located on the spacer glass 2 Within the range of the orthographic projection of the upper opening), a cavity is formed after the base 3, the transparent silica gel plate 9, the silica gel pressing sheet 8, the spacer slide 2 and the fixed slide 1 are bonded together by optical glue , for detecting the inner cavity; the two ends of the central opening on the silica gel pressing sheet 8 are respectively provided with a smaller opening, and these two smaller openings are preferably within the range of the orthographic projection of the central opening of the septum 2 (at least also need to have partial overlap), the position of two smaller openings on the corresponding silica gel pressing sheet 8 on the described transparent silica gel plate 9 is also provided with the opening of the same size, on the described transparent silica gel plate 9 and on the silica gel pressing sheet 8 Four smaller openings constitute the channel for urine to enter and flow out of the detection inner chamber; the base 3 is provided with a sample inlet and a sample outlet that communicate with the above-mentioned passage for urine to enter and exit the detection inner cavity, so Described sample inlet and sample outlet are connected with sample inlet pipe 4 and sample outlet pipe 5 respectively;

At the bottom of the base 3, there is also an action channel 11 communicating with the bottom part of the bottom solid-phase support structure. Negative pressure system (such as diaphragm pump or plunger pump) is connected, and the transparent silica gel plate 9 is approached in the detection cavity by applying force to the action channel 11 by using the positive or negative pressure acting on the transparent silica gel plate 9 The up and down reciprocating motion of the fixed glass slide 1 or away from the fixed glass slide 1.

The specific working process of using the above counting pool is as follows:

1) The sampling pump is working, open the control valve of the sampling channel, open the control valve of the sampling channel, so that the urine enters the small opening on the side of the transparent silica gel plate 9 through the sampling tube 4, and then enters the silica gel sheet 8 The smaller opening on the same side as the smaller opening on the above-mentioned transparent silica gel plate 9, then enters the detection inner cavity, and closes the control valve of the sampling channel after the detection inner cavity is filled with the sample to be tested;

2) Open the control valve of the action channel 11, open the high-pressure valve of the action channel 11, apply a pressure of 2 atmospheres to the transparent silica gel plate 9 through the action channel 11, until the transparent silica gel plate 9 is completely pushed to the fixed glass slide 1, so that the urine Formed components such as cells in the medium settle on the transparent silica gel plate 9 (as shown in Figure 6), maintain the pressure of the acting channel 11, and close the control valve of the sample outlet channel;

3) The microscope 6 focuses under the condition that the light source 7 exists, and completes the detection;

4) Open the control valve of the sample outlet channel, open the control valve of the sample injection channel, close the high pressure valve of the action channel 11, open the negative pressure valve of the action channel 11, and provide a negative pressure of about 55Kpa to the transparent silica gel plate 9, ready for cleaning and testing lumen;

5) When cleaning, turn on the sampling pump, pump the cleaning solution into the sampling channel, under the negative pressure of the action channel 11, the transparent silica gel plate 9 leaves the fixed glass slide 1 and returns to the initial position and stops, and then proceeds to the subsequent cleaning procedure .

7 to 10 are illustrations of the second embodiment of the present invention. The difference from the first embodiment is that the solid phase support structure at the bottom is different. In this embodiment, the bottom solid-phase support structure is specifically composed of a transparent silica gel plate 9 placed on the lower layer, a silica gel pressing sheet 8 placed on the transparent silica gel plate 9, and a moving glass slide 12 placed between the two , the silica gel pressing sheet 8 is provided with an opening (the opening is within the range of the orthographic projection of the opening on the interval glass 2), and the moving glass 12 is located within the range of the orthographic projection of the opening on the silica gel pressing sheet 8 and is For the action channel 11; after the base 3, the transparent silica gel plate 9, the moving glass slide 12, the silica gel pressing plate 8, the spacer glass slide 2 and the fixed slide glass 1 are bonded together to form a void Cavity, for detecting inner cavity; Also offer a smaller opening respectively at the two ends of the central opening on the silica gel pressing sheet 8, these two smaller openings are preferably within the orthographic range of the central opening of the septum 2 ( At least it needs to partially overlap with the opening on the glass slide 2), and the transparent silica gel plate 9 is also provided with openings of the same size at the positions corresponding to the two smaller openings on the silica gel pressing sheet 8. The transparent silica gel plate 9 The four smaller openings on the top and the silica gel pressing sheet 8 form passages for urine to enter and flow out of the detection inner chamber; on the base 3, there is an inlet connected to the passage for the above-mentioned passage of urine into and out of the detection chamber. A sample hole and a sample outlet, the sample inlet and the sample outlet are connected to the sample inlet tube 4 and the sample outlet tube 5 respectively.

When a diaphragm pump or a plunger pump is used to apply a force to the action channel 11, as the transparent silica gel plate 9 moves upward, the moving slide 12 passes through the opening in the center of the silica gel pressing plate 8 and the opening in the center of the separating glass 2, and moves toward the The direction of the fixed glass slide 1 moves, and finally the cells and other tangible substances in the urine are fixed on the moving glass slide 12, as shown in FIG. 10 .

The specific working process of the counting pool with the above structure is as follows:

1) The sampling pump is working, open the control valve of the sampling channel, open the control valve of the sampling channel so that the urine enters the small opening on the side of the transparent silica gel plate 9 through the sampling tube 4, and then enters the silica gel pressing sheet 8. The smaller opening on the same side as the smaller opening on the above-mentioned transparent silica gel plate 9 enters the detection inner cavity, and the control valve of the sampling channel is closed after the detection inner cavity is filled with the sample to be tested;

2) Open the control valve of the action channel 11, open the high-pressure valve of the action channel 11, apply a pressure of 2 atmospheres to the transparent silica gel plate 9 through the action channel 11, until the moving slide 12 is completely pushed to the fixed slide 1, so that the urine Formed components such as cells in the cell settle on the moving slide 12 (as shown in Figure 10), keep the pressure of the acting channel 11, and close the control valve of the sample outlet channel;

3) The microscope 6 focuses under the condition that the light source 7 exists, and completes the detection;

4) Open the control valve of the sample outlet channel, open the control valve of the sample injection channel, close the high pressure valve of the action channel 11, open the negative pressure valve of the action channel 11, provide a negative pressure of about 55Kpa to the silica gel, and prepare to clean the detection channel;

5) When cleaning, turn on the sampling pump, pump the cleaning solution into the sampling channel, under the negative pressure of the action channel 11, the moving slide 12 stops when it leaves the fixed slide 1 and returns to the initial position, and then proceeds to the subsequent cleaning procedure .

11 to 14 are illustrations of the third embodiment of the present invention, which is different from the first and second embodiments in that the solid phase support structure at the bottom is different. In this embodiment, the bottom solid-phase support structure is specifically composed of a moving glass slide 12, a partition 13, a transparent silica gel plate 9 and a silica gel pressing sheet 8 arranged sequentially from bottom to top, and the silica gel pressing sheet 8 and Openings are provided on the partition 13 (the openings of the silica gel pressing sheet 8 and the partition 13 overlap with the opening on the partition glass 2 at least), and the moving glass 12 is positioned at the front of the opening on the partition 13. Within the projection range and directly facing the action channel 11; after the base 3, the partition plate 13, the moving glass slide 12, the transparent silica gel plate 9, the silica gel pressing sheet 8, the spacer slide 2 and the fixed slide 1 are coated with optical glue After bonding together (moving glass slide 12 is bonded on the back side of transparent silica gel plate) form a cavity, for detecting inner cavity; Also open a smaller one at the two ends of central opening on silica gel pressing sheet 8 respectively. The openings, these two smaller openings are preferably within the range of the orthographic projection of the central opening of the septum 2 (at least partially overlapping with the opening on the septum 2), and the corresponding silica gel pressure on the transparent silica gel plate 9 There are also openings of the same size at the positions of the two smaller openings on the sheet 8, and the four smaller openings on the transparent silica gel plate 9 and the silica gel pressing sheet 8 constitute the detection cavity for urine to enter and flow out. Channel; the base 3 is provided with a sample inlet and a sample outlet that are communicated with the passage for the urine to enter and exit the detection cavity, and the sample inlet and the sample outlet are connected to the sample inlet 4 and the outlet respectively. The sample tube 5 is connected.

When positive pressure is applied to the moving glass 12 through the action channel 11, the opening on the partition 13 reserves a space for the moving glass 12 to reciprocate up and down, and as the moving glass 12 moves, the transparent silica gel plate 9 is pushed toward the fixed glass slide 1, thereby the formed components such as cells in the urine are settled on the transparent silica gel plate 9, as shown in FIG. 14 .

The specific working process of the counting pool with the above structure is as follows:

1) The sampling pump is working, open the control valve of the sampling channel, open the control valve of the sampling channel so that the urine enters the small opening on the side of the transparent silica gel plate 9 through the sampling tube 4, and then enters the silica gel pressing sheet 8. The smaller opening on the same side as the smaller opening on the above-mentioned transparent silica gel plate 9 enters the detection inner cavity, and the control valve of the sampling channel is closed after the detection inner cavity is filled with the sample to be tested;

2) Open the control valve of the action channel 11, open the high-pressure valve of the action channel 11, apply a pressure of 2 atmospheres to the moving glass slide 12 through the action channel 11, until the moving glass slide 12 drives the silica gel to completely push the fixed glass slide 1, so that Formed components such as cells in the urine are fixed on the transparent silica gel plate 9 (as shown in Figure 14), maintain the pressure of the acting channel 11, and close the control valve of the liquid outlet channel;

3) The microscope 6 focuses under the condition that the light source 7 exists, and completes the detection;

4) Open the control valve of the sample outlet channel, open the control valve of the sample injection channel, close the high pressure valve of the action channel 11, open the negative pressure valve of the action channel 11, and provide a negative pressure of about 55Kpa to the moving slide 12, ready for cleaning and testing aisle;

5) When cleaning, turn on the sampling pump and pump the cleaning solution into the sampling channel. Under the negative pressure of the action channel 11, the moving glass slide 12 drives the silica gel to leave the fixed glass slide 1 and return to the initial position to stop, and then proceed to the subsequent steps. Cleaning program.

Claims (5)

1. the arena counting chamber comprises pedestal (3), is provided with bottom solid phase support structure on it from the bottom to top, every slide (2) with decide slide (1), and central authorities offer opening every slide (2), it is characterized in that:

Described bottom solid phase support structure is by the transparent silicon offset plate (9) that places lower floor, and silica gel compressing tablet (8) placed on it is formed, offer opening on this silica gel compressing tablet (8), with described pedestal (3), transparent silicon offset plate (9), silica gel compressing tablet (8), every slide (2) with decide slide (1) back that bonds together and form a cavity, be to detect inner chamber; Offer for sample liquid into and out of the passage that detects inner chamber at silica gel compressing tablet (8) and transparent silicon offset plate (9), offer and above-mentioned for sample liquid sample holes and the sample outlet hole into and out of the channel connection that detects inner chamber at pedestal (3), described sample holes and sample outlet hole respectively with sample introduction pipe (4) with go out sample pipe (5) and be connected;

On pedestal (3), also be provided with one with the effect passage (11) of the orlop members of bottom solid phase support structure, by this effect passage (11) power of applying bottom solid phase support structure is moved back and forth in the detection inner chamber.

2. arena counting chamber according to claim 1, it is characterized in that: described bottom solid phase support structure is by the transparent silicon offset plate (9) that places lower floor, place the silica gel compressing tablet (8) on the transparent silicon offset plate (9), and place moving slide (12) between the two to form, offer opening on the described silica gel compressing tablet (8), described moving slide (12) is positioned at the orthogonal projection scope of silica gel compressing tablet (8) upper shed and is right against effect passage (11); With described pedestal (3), transparent silicon offset plate (9), moving slide (12), silica gel compressing tablet (8), every slide (2) with decide slide (1) cavity of back formation that bonds together, it is the detection inner chamber; Offer for sample liquid into and out of the passage that detects inner chamber at silica gel compressing tablet (8) and transparent silicon offset plate (9), offer and above-mentioned for sample liquid sample holes and the sample outlet hole into and out of the channel connection that detects inner chamber at pedestal (3), described sample holes and sample outlet hole respectively with sample introduction pipe (4) with go out sample pipe (5) and be connected.

3. arena counting chamber according to claim 1, it is characterized in that: described bottom solid phase support structure is made up of the moving slide (12) that sets gradually from the bottom up, dividing plate (13), transparent silicon offset plate (9) and silica gel compressing tablet (8), all offer opening on described silica gel compressing tablet (8) and the dividing plate (13), described moving slide (12) is positioned at the orthogonal projection scope of silica gel compressing tablet (8) and dividing plate (13) upper shed and is right against effect passage (11); With described pedestal (3), dividing plate (13), moving slide (12), transparent silicon offset plate (9), silica gel compressing tablet (8), every slide (2) with decide slide (1) cavity of back formation that bonds together, it is the detection inner chamber; Offer for sample liquid into and out of the passage that detects inner chamber at silica gel compressing tablet (8) and transparent silicon offset plate (9), offer and above-mentioned for sample liquid sample holes and the sample outlet hole into and out of the channel connection that detects inner chamber at pedestal (3), described sample holes and sample outlet hole respectively with sample introduction pipe (4) with go out sample pipe (5) and be connected.

4. according to claim 1,2 or 3 described arena counting chambers, it is characterized in that: in the solid phase support structure of described bottom, replace transparent silicon offset plate (9) with plastic sheeting.

5. according to claim 1,2 or 3 described arena counting chambers, it is characterized in that: described effect passage (11) is connected with the system that can change the detection chamber pressure.

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