CN103110815A - Traditional Chinese medicine composition for treating type II diabetes and preparation method thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine composition for treating type II diabetes. , composed of Digupi, can relieve the thirst symptoms caused by type II diabetes, and at the same time can regulate blood sugar, and can treat various complications caused by diabetes, and has a significant effect on type II diabetes.

Description

A kind of traditional Chinese medicine composition for treating type II diabetes and its preparation method

technical field

The invention discloses a traditional Chinese medicine composition, especially a traditional Chinese medicine composition for treating type II diabetes and a preparation method thereof. It belongs to the field of traditional Chinese medicine.

Background technique

Diabetes (diabetes) is a disease in which glucose in the blood tends to accumulate too much. Its symptoms are usually referred to as "hyperglycemia" in China, and together with hypertension and hyperlipidemia, they are called "three highs". Functional disorder, microbial infection and its toxins, free radical toxins, mental factors and other pathogenic factors act on the body to cause hypofunction of islets of pancreas, insulin resistance, etc., resulting in a series of metabolic disorders such as sugar, protein, fat, water and electrolytes sign. It is nicknamed "Silent Killer" abroad, especially "adult diabetes", and the prevalence rate of middle-aged people over the age of 40 is particularly high.

Diabetes is divided into type I diabetes, type II diabetes, gestational diabetes and other special types of diabetes.

① Type I diabetes is an autoimmune disease. Autoimmune diseases are caused by the body's immune system attacking itself. In diabetics, the immune system attacks and kills the beta cells of the pancreas that secrete their own insulin. As a result, the pancreas does not produce enough insulin. Due to the lack of insulin secretion, patients need to rely on exogenous insulin supplements to maintain life.

② Type II diabetes is adult-onset diabetes, which usually occurs after the age of 35 to 40, accounting for more than 90% of diabetic patients. The ability to produce insulin in the patient's body is not completely lost, and some patients even produce too much insulin in the body, but the effect of insulin is greatly reduced, so the insulin in the patient's body is relatively deficient.

③ Gestational diabetes refers to diabetes that women develop during pregnancy. Clinical data show that about 2-3% of women will develop diabetes during pregnancy, and the diabetes will disappear automatically after pregnancy. Gestational diabetes is more likely to occur in obese and advanced maternal age. Nearly 30% of women with gestational diabetes may later develop type II diabetes.

Type II diabetes has stronger genetic and environmental factors, and shows significant heterogeneity. Its typical cases may show polyuria, polydipsia, polyphagia, emaciation, etc., that is, "three more and one less" symptoms. Diabetes ( Blood sugar) Once it is not well controlled, it will cause complications, leading to failure and lesions of the kidneys, eyes, feet and other parts, and it cannot be cured.

Nowadays, the most commonly used treatment method is to take hypoglycemic drugs such as metformin, which treats the symptoms but not the root cause. The easier it is for the patient's body to develop drug resistance.

TCM treatment of diabetes pays attention to overall regulation, and is obviously superior to Western medicine in improving symptoms and other aspects. It is suitable for non-insulin-dependent diabetes mellitus (type II diabetes mellitus) and those with chronic vascular and neurological complications.

In the existing method for the treatment of type II diabetes by traditional Chinese medicine, Chinese invention patent application CN 02133022.0 disclosed a kind of " a kind of traditional Chinese medicine preparation celandine extract for treating diabetes" on March 2, 2003. The preparation method of the medicine is to take 600g of the aerial part of celandine in June as a dosage, wash it, chop it up, decoct it in water for three times, and concentrate it to 1000ml solution; (2) add 300ml absolute alcohol after the concentrated solution cools down, After standing still for 12 hours, remove the protein, take soil clear liquid and add normal water to make 1200ml of medicinal solution with a concentration of 50%, or 1000ml of medicinal solution with a concentration of 30%, or a medicinal solution with a concentration of 20%. Liquid 2400ml. Its function is to treat diabetic peripheral neuropathy. It belongs to the extract solution for treating a complication of diabetes.

Contents of the invention

Aiming at the deficiencies of the existing methods for treating type II diabetes, one of the purposes of the present invention is to provide a Chinese medicine composition for treating type II diabetes, which has no side effects during the treatment of type II diabetes, and can treat the disease caused by diabetes while regulating blood sugar. Diabetes causes a variety of uncomfortable symptoms.

Another object of the present invention is to provide a preparation method of the above-mentioned traditional Chinese medicine composition for treating type II diabetes.

Above-mentioned purpose of the present invention is achieved through the following technical solutions:

A traditional Chinese medicine composition for treating type II diabetes. The preferred solution is prepared from the main raw materials in the following weight proportions: 10-30 parts of astragalus, 9-30 parts of Codonopsis pilosula, 12-30 parts of rehmannia glutinosa, and 15-30 parts of Chinese yam 6-12 parts of Polygonatum Polygonatum, 5-15 parts of Lycium barbarum, 10-20 parts of Asparagus, 10-15 parts of Scrophulariaceae, 6-15 parts of Ligustrum lucidum, 7-15 parts of Pueraria lobata, 20-50 parts of bitter gourd powder , 3-9 parts of black medicine, 9-15 parts of mulberry, 15-30 parts of Digupi (preferably, 15.5-29.5 parts of Digupi).

A traditional Chinese medicine composition for treating type II diabetes. A more preferred solution is prepared from the main raw materials in the following weight ratios: 12-28 parts of astragalus, 12-25 parts of Codonopsis pilosula, 14-28 parts of rehmannia glutinosa, and 16-28 parts of Chinese yam. 28 parts, Polygonatum 8-11 parts, Lycium barbarum 6-14 parts, Asparagus 12-18 parts, Scrophulariaceae 12-14 parts, Ligustrum lucidum 8-13 parts, Pueraria 8-14 parts, Bitter melon powder 22-48 parts 6-8 parts, 6-8 parts of black medicine, 10-14 parts of mulberry, 16-28 parts of Digupi.

A traditional Chinese medicine composition for treating type II diabetes. The best solution is prepared from the main raw materials in the following weight proportions: 20 parts of astragalus, 18 parts of codonopsis, 18 parts of rehmannia glutinosa, 18 parts of yam, 10 parts of Polygonatum odoratum, 12 parts of medlar, 14 parts of asparagus, 13 parts of Scrophulariaceae, 12 parts of Ligustrum lucidum, 12 parts of kudzu root, 30 parts of bitter gourd powder, 7 parts of black medicine, 12 parts of mulberry, and 18 parts of Digupi.

A preparation method of a traditional Chinese medicine composition for treating type II diabetes, the preparation steps are as follows:

①Put astragalus, Codonopsis pilosula, Rehmannia glutinosa, Chinese yam, Polygonatum odoratum, Lycium barbarum, Asparagus, Scrophulariaceae, Ligustrum lucidum, Pueraria lobata, black medicine, mulberry, Digupi into a casserole and soak in cold water for 20-40 minutes;

②Fry the soaked medicinal materials over high heat, turn to low heat and fry for 25-35 minutes, and filter out the medicinal liquid;

③After filtering out the medicinal liquid, add cold water to boil again, turn to low heat and fry for 20-40 minutes, add bitter melon powder one minute before turning off the heat, filter out the medicinal liquid, mix with ②Chinese medicinal liquid, and divide into two parts.

The preparation method of the above-mentioned traditional Chinese medicine composition, step ① soak in cold water for 20-40 minutes (preferably 25-35 minutes, more preferably 30 minutes).

In the preparation method of the above-mentioned traditional Chinese medicine composition, step ② turn to low heat and fry for 25-35 minutes (preferably 26-32 minutes, more preferably 30 minutes).

In the preparation method of the above-mentioned traditional Chinese medicine composition, step ③ turns to low heat and fry for 20-40 minutes (preferably 25-35 minutes, more preferably 30 minutes).

The present invention has remarkable blood sugar regulating effect on type II diabetes. In order to achieve the best therapeutic effect of the present invention, it is taken twice a day, half an hour after meals in the morning and evening respectively.

The pharmacological efficacy of each raw material drug is respectively:

Radix Astragali: sweet in taste, warm in nature. Beneficial to qi solidification, diuresis, detumescence, and quenching thirst. It is used for qi deficiency, fatigue, internal heat, diabetes and other symptoms.

Codonopsis: sweet in taste, flat in nature. It has the effect of invigorating qi and promoting body fluid, and is a common qi-invigorating medicine in clinical practice.

Rehmannia glutinosa: sweet in taste, cold in nature. It is the fresh or dried root tuber of Rehmannia glutinosa of the Scrophulariaceae plant, commonly known as "shengdi". It has the effects of clearing away heat and cooling blood, promoting body fluid, and is used for pyrexia with crimson tongue and polydipsia.

Chinese yam: sweet in taste, flat in nature. It has the effects of invigorating the spleen, improving ears and eyesight, nourishing the middle and nourishing qi, quenching thirst and promoting body fluid, nourishing the spleen and nourishing the stomach, and controlling the desire to eat. It is used for thirsty drinking, fatigue, and cardiovascular fat deposition.

Polygonatum: sweet in taste, flat in nature. It has the effect of promoting body fluid and quenching thirst, and is used for dry throat and thirst, muscle spasm and pain.

Lycium barbarum: sweet in taste, flat in nature. It has the effects of improving eyesight, strengthening tendons, and nourishing essence. It is used for fatigue, fatigue, blurred vision, thirsty and thirsty.

Radix Asparagus: bitter in taste, flat in nature. It is a tonic and lung-clearing medicine. It has the effects of nourishing yin and moistening dryness, reducing swelling, and quenching thirst. It is used for sore throat, constipation, febrile disease that hurts yin, and internal heat to quench one's thirst.

Radix Scrophulariae: sweet, bitter, slightly cold in nature. It has the effects of promoting body fluid, lowering blood pressure and lowering blood sugar, and has a promoting effect on cardiovascular. It is used for diabetes, thirsty, crimson tongue, irritability, and restless sleep at night.

Ligustrum lucidum: sweet in the mouth, bitter, slightly cool in nature. For tonic. It has the effects of nourishing the liver and kidney, black beard and eyesight, lowering blood sugar, lowering blood fat and anti-arteriosclerosis. It is used for dizziness, soreness of the waist and knees, liver and kidney yin deficiency, high blood sugar, high blood fat, high blood pressure and other symptoms.

Radix Puerariae: sweet, pungent, cool in nature. It has the effects of reducing vascular resistance, improving microcirculation, quenching thirst, lowering blood sugar and lipids, and is used for symptoms such as hyperglycemia, hyperlipidemia, and hypertension.

Bitter melon powder: Bitter melon is ground into powder, also known as bitter melon, bitter in taste and cold in nature. It has the effects of improving eyesight and detoxification, lowering blood pressure and lowering blood sugar, nourishing qi, preventing atherosclerosis, and protecting the heart. "Compendium of Materia Medica" records that bitter gourd has the effect of "removing evil heat, relieving fatigue, clearing heart and improving eyesight", and is used for high blood sugar. embolism.

Wuyao: pungent in taste, warm in nature. It has the effects of promoting qi and relieving pain, warming the kidney and dispelling cold, and relieving compound muscle spasm pain.

Mulberry: sweet, sour, cold in nature. It is the fruit ear of the mulberry plant of the family Moraceae. It has the effects of nourishing blood, promoting body fluid, moistening dryness, and improving eyesight. It can be used for thirsty wounds, prevent human arteriosclerosis, and has auxiliary effects on treating diabetes, anemia, high blood pressure, high blood fat and other diseases.

Radix Digupi: sweet in the mouth, cold in nature. It has the effect of clearing heat and cooling blood, lowering blood sugar and blood fat, and has obvious blood pressure lowering effect, which can be used for high blood pressure, high blood fat and high blood sugar.

In fact, the advantage of traditional Chinese medicine in the treatment of diabetes is not only in lowering blood sugar, but more importantly, through the method of syndrome differentiation and comprehensive measures to relieve the clinical symptoms of patients, prevent and treat various chronic complications, and prolong the life of diabetic patients.

The present invention has remarkable effects on type II diabetes, and with the three-dimensional cooperation of diet control, moderate exercise, and maintaining a good mood, patients with mild type II diabetes can completely stop taking the drug in the later stage.

Detailed ways

The technical solutions of the present invention will be described in detail below in conjunction with the examples and experimental examples, but the scope of protection is not limited thereto.

Example 1

Astragalus 10 grams, Codonopsis 9 grams, Rehmannia glutinosa 12 grams, Chinese yam 15 grams, Polygonatum 6 grams, Lycium barbarum 5 grams, Asparagus 10 grams, Scrophulariaceae 10 grams, Ligustrum lucidum 6 grams, Pueraria 7 grams, bitter melon powder 20 grams , 3 grams of black medicine, 9 grams of mulberry, 15 grams of Digupi.

①Put astragalus, Codonopsis pilosula, Rehmannia glutinosa, Chinese yam, Polygonatum odoratum, Lycium barbarum, Asparagus, Scrophulariaceae, Ligustrum lucidum, Pueraria lobata, black medicine, mulberry, Digupi into a casserole and soak in cold water for 20 minutes;

②Fry the soaked medicinal materials on high heat, turn to low heat and fry for 25 minutes, and filter out the medicinal liquid;

③After filtering out the medicinal liquid, add cold water to boil again, turn to low heat and fry for 20 minutes, add bitter melon powder one minute before turning off the heat, filter out the medicinal liquid, mix with ②Chinese medicinal liquid, and divide into two parts.

Example 2

Astragalus 12 grams, Codonopsis 12 grams, Rehmannia glutinosa 14 grams, Chinese yam 16 grams, Polygonatum 8 grams, Lycium barbarum 6 grams, Asparagus 12 grams, Scrophulariae 12 grams, Ligustrum lucidum 8 grams, Pueraria 8 grams, bitter melon powder 22 grams , 6 grams of black medicine, 10 grams of mulberry, 16 grams of Digupi.

①Put astragalus, Codonopsis pilosula, Rehmannia glutinosa, Chinese yam, Polygonatum odoratum, Lycium barbarum, Asparagus, Scrophulariaceae, Ligustrum lucidum, Pueraria lobata, black medicine, mulberry, Digupi into a casserole and soak in cold water for 25 minutes;

②Fry the soaked medicinal materials on high heat, turn to low heat and fry for 26 minutes, and filter out the medicinal liquid;

③After filtering out the medicinal liquid, add cold water to boil again, turn to low heat and fry for 25 minutes, add bitter melon powder one minute before turning off the heat, filter out the medicinal liquid, mix with ②Chinese medicinal liquid, and divide into two parts.

Example 3

Astragalus 28 grams, Codonopsis 25 grams, Rehmannia glutinosa 28 grams, Chinese yam 28 grams, Polygonatum 11 grams, Lycium barbarum 14 grams, Asparagus 18 grams, Scrophularia scrophulariae 14 grams, Ligustrum lucidum 13 grams, Pueraria 14 grams, bitter melon powder 48 grams , 8 grams of black medicine, 14 grams of mulberry, 28 grams of Digupi.

①Put astragalus, Codonopsis pilosula, Rehmannia glutinosa, Chinese yam, Polygonatum odoratum, Lycium barbarum, Asparagus, Scrophulariaceae, Ligustrum lucidum, Pueraria lobata, black medicine, mulberry, Digupi into a casserole and soak in cold water for 35 minutes;

②Fry the soaked medicinal materials on high heat, turn to low heat and fry for 32 minutes, and filter out the medicinal liquid;

③After filtering out the medicinal liquid, add cold water to boil again, turn to low heat and fry for 35 minutes, add bitter melon powder one minute before turning off the heat, filter out the medicinal liquid, mix with ②Chinese medicinal liquid, and divide into two parts.

Example 4

Astragalus 30 grams, Codonopsis 30 grams, Rehmannia glutinosa 30 grams, Chinese yam 30 grams, Polygonatum 12 grams, Lycium barbarum 15 grams, Asparagus 20 grams, Scrophulariaceae 15 grams, Ligustrum lucidum 15 grams, Pueraria 15 grams, bitter melon powder 50 grams , 9 grams of black medicine, 15 grams of mulberry, 30 grams of Digupi.

①Put astragalus, Codonopsis pilosula, Rehmannia glutinosa, Chinese yam, Polygonatum odoratum, Lycium barbarum, Asparagus, Scrophulariaceae, Ligustrum lucidum, Pueraria lobata, black medicine, mulberry, Digupi into a casserole and soak in cold water for 40 minutes;

②Fry the soaked medicinal materials on high heat, turn to low heat and fry for 35 minutes, and filter out the medicinal liquid;

③After filtering out the medicinal liquid, add cold water to boil again, turn to low heat and fry for 40 minutes, add bitter melon powder one minute before turning off the heat, filter out the medicinal liquid, mix with ②Chinese medicinal liquid, and divide into two parts.

Example 5

Astragalus 20 grams, Codonopsis 18 grams, Rehmannia glutinosa 18 grams, Chinese yam 18 grams, Polygonatum 10 grams, Lycium barbarum 12 grams, Asparagus 14 grams, Scrophulariaceae 13 grams, Ligustrum lucidum 12 grams, Pueraria 12 grams, bitter melon powder 30 grams , 7 grams of black medicine, 12 grams of mulberry, 18 grams of Digupi.

①Put astragalus, Codonopsis pilosula, Rehmannia glutinosa, Chinese yam, Polygonatum odoratum, Lycium barbarum, Asparagus, Scrophulariaceae, Ligustrum lucidum, Pueraria lobata, black medicine, mulberry, Digupi into a casserole and soak in cold water for 30 minutes;

②Fry the soaked medicinal materials over high heat, turn to low heat and fry for 30 minutes, and filter out the medicinal liquid;

③After filtering out the medicinal liquid, add cold water to boil again, turn to low heat and fry for 30 minutes, add bitter melon powder one minute before turning off the heat, filter out the medicinal liquid, mix with ②Chinese medicinal liquid, and divide into two parts.

Method of use: In order to achieve the best therapeutic effect of the present invention, take it twice a day, half an hour after meals in the morning and evening respectively.

Typical case one

Sun XX, male, 45 years old, lives in the Development Zone of Heze City, Shandong Province. Symptoms: Type II diabetes with mild hypertension, without coronary artery disease and other complications of diabetes. Age of illness: 2 years. Visiting time: January 10, 2011; taking the traditional Chinese medicine of the present invention for 6 months, combined with moderate exercise and diet control, the blood sugar has been stable and normal without large fluctuations; Continue to exercise moderately and control diet, and have not taken hypoglycemic drugs so far.

Typical case two

Qiao XX, male, 55 years old, lived in Jianglou Town, Huimin County, Binzhou City, Shandong Province. Symptoms: Polyuria, dry mouth, and polydipsia at the beginning, and then symptoms of general fatigue and dizziness. The random blood sugar was 3mmol/L after the hospital test, and then diagnosed as type II diabetes. Age of illness: 5 years. Visiting time: April 5, 2011; taking the traditional Chinese medicine of the present invention, twice a day, after taking for 5 days, dry mouth symptoms alleviated, and dizziness symptoms disappeared; after taking for 2 months, blood sugar returned to stable and normal; insisted on taking 7 For one month, with moderate exercise and diet control, the blood sugar has been stable, and there was no adverse reaction during the period.

Typical case three

Liu XX, female, 32 years old, lived in Dingshui Town, Yanggu County, Liaocheng City, Shandong Province. Symptoms: It was gestational diabetes at first, and later turned into type II diabetes. The blood sugar was 6.7 on an empty stomach and 23.5 after meals. Age of illness: 3 years. Visiting time: June 5, 2011; take the traditional Chinese medicine of the present invention twice a day, and cooperate with appropriate amount of exercise and diet control, take it for 3 months, and the blood sugar tends to be stable; insist on taking it for 5 months, during which the blood sugar has no Significant changes; gradually reduce the dose for 3 months, blood sugar is still stable, blood sugar is still stable since drug withdrawal.

In order to verify the effect of the medicament of the present invention, we conducted multiple drug experiments (Example 5), and accurately performed statistical and mathematical analysis on the data. The specific experimental results are as follows:

Compared with other hypoglycemic drugs, the Chinese medicine composition of the present invention is more comprehensive in treatment. Clinical trials have proved that the effective rate of the Chinese medicine composition of the present invention is 95%, and the cure rate is 40%, which is higher than that of oral western medicine hypoglycemic drugs. 26.7%, and the Chinese medicine composition of the present invention can also regulate physical symptoms such as dry mouth and weight loss caused by type II diabetes and complications. Patients with mild type II diabetes who take the traditional Chinese medicine composition of the present invention combined with moderate exercise and diet control can completely stop taking the medicine in the later stage, and the blood sugar can still remain stable. It is an ideal drug for treating type II diabetes.

The prescription of this application is a proven prescription of a famous old doctor of traditional Chinese medicine, and has the prospect of industrialization implementation. The completion of the invention benefited from the support of the key discipline of the 12th Five-Year Plan of Shandong Province—the discipline of economic law.

Finally, it should be noted that the embodiments are only the best specific implementation of the present invention, and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, it is still The technical solutions recorded in the foregoing embodiments may be modified, or some technical features thereof may be equivalently replaced. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims (7)

1. a Chinese medicine composition for the treatment of type Ⅱdiabetes mellitus, is characterized in that, mainly the raw material by the following weight proportioning is prepared from: Radix Astragali 10-30 part, Radix Codonopsis 9-30 part, Radix Rehmanniae 12-30 part, Rhizoma Dioscoreae 15-30 part, Rhizoma Polygonati Odorati 6-12 part, Fructus Lycii 5-15 part, Radix Asparagi 10-20 part, Radix Scrophulariae 10-15 part, Fructus Ligustri Lucidi 6-15 part, Radix Puerariae 7-15 part, Fructus Momordicae charantiae powder 20-50 part, Radix Linderae 3-9 part, Fructus Mori 9-15 part, Cortex Lycii 15-30 part.

2. Chinese medicine composition according to claim 1, is characterized in that, mainly the raw material by the following weight proportioning is prepared from: Radix Astragali 12-28 part, Radix Codonopsis 12-25 part, Radix Rehmanniae 14-28 part, Rhizoma Dioscoreae 16-28 part, Rhizoma Polygonati Odorati 8-11 part, Fructus Lycii 6-14 part, Radix Asparagi 12-18 part, Radix Scrophulariae 12-14 part, Fructus Ligustri Lucidi 8-13 part, Radix Puerariae 8-14 part, Fructus Momordicae charantiae powder 22-48 part, Radix Linderae 6-8 part, Fructus Mori 10-14 part, Cortex Lycii 16-28 part.

3. Chinese medicine composition according to claim 1, is characterized in that, mainly the raw material by the following weight proportioning is prepared from: 20 parts of the Radixs Astragali, 18 parts of Radix Codonopsis, 18 parts of Radix Rehmanniae, 18 parts of Rhizoma Dioscoreaes, 10 parts of Rhizoma Polygonati Odorati, 12 parts of Fructus Lycii, 14 parts of Radix Asparagis, 13 parts of Radix Scrophulariaes, 12 parts of Fructus Ligustri Lucidi, 12 parts of Radix Puerariaes, 30 parts of Fructus Momordicae charantiae powders, 7 parts of the Radixs Linderae, 12 parts, Fructus Mori, 18 parts of Cortex Lycii.

4. the preparation method of according to claim 1-3 arbitrary described Chinese medicine compositions, is characterized in that, preparation process is as follows:

1. the Radix Astragali, Radix Codonopsis, Radix Rehmanniae, Rhizoma Dioscoreae, Rhizoma Polygonati Odorati, Fructus Lycii, Radix Asparagi, Radix Scrophulariae, Fructus Ligustri Lucidi, Radix Puerariae, the Radix Linderae, Fructus Mori, Cortex Lycii are put into marmite and used cold water soak 20-40 minute;

2. the medical material that soaks is fried open with big fire, turns little fire and fried in shallow oil 25-35 minute, leach medicinal liquid;

3. after leaching medicinal liquid, again add cold water to be fried open, turn little fire and fried in shallow oil 20-40 minute, close fire and added Fructus Momordicae charantiae powder in last minute, leach medicinal liquid, mix with 2. herb liquid, be divided into two parts.

5. the preparation method of Chinese medicine composition according to claim 4, is characterized in that, 1. step uses cold water soak 20-40 minute (preferred 25-35 minute, be more preferably 30 minutes).

6. the preparation method of Chinese medicine composition according to claim 4, is characterized in that, 2. step turns little fire and fry in shallow oil 25-35 minute (preferred 26-32 minute, be more preferably 30 minutes).

7. the preparation method of Chinese medicine composition according to claim 4, is characterized in that, 3. step turns little fire and fry in shallow oil 20-40 minute (preferred 25-35 minute, be more preferably 30 minutes).