CN103092090B - Sequencing control system - Google Patents

Abstract

The invention relates to the field of automatic control and provides a sequencing control system. The system includes: a first sequencing reaction unit, a first image acquisition unit, a second sequencing reaction unit, a second image acquisition unit and a control unit. Among them, the first sequencing reaction unit is used to control the sequencing reaction of the first sequencing reaction chamber; the first image acquisition unit is used to control the image acquisition component to detect and collect signals from the first sequencing reaction chamber to obtain signal data; the second The sequencing reaction unit is used to control the sequencing reaction of the second sequencing reaction chamber; the second image acquisition unit is used to control the image acquisition component to detect and collect signals from the second sequencing reaction chamber to obtain signal data; the control unit is used to control the second sequencing reaction chamber A sequence reaction unit and the first image acquisition unit perform multiple cycles of work, and are used to control the second sequence reaction unit and the second image acquisition unit to perform multiple cycles of work. The sequencing control system of the present invention can realize rapid sequencing and improve the utilization rate of the sequencing system.

Description

A sequencing control system

technical field

The present invention relates to the field of automatic control, more specifically, to a sequencing control system.

Background technique

At present, there is a scene of contention in the sequencing industry. Different companies have different sequencing principles, such as: massively parallel signature sequencing (Massively Parallel Signature Sequencing, MPSS), polymerase cloning (Polony Sequencing), 454 pyrosequencing (454 pyrosequencing) , Illumina (Solexa) sequencing, ABI SOLiD sequencing, Ion semiconductor sequencing, DNA nanoball sequencing, etc. Although the sequencing principles are different, the sequencing process is basically the same. First, the sample is fixed on a solid-phase carrier; then, the solid-phase carrier is installed on the sequencing reaction platform in the sequencing system to form a sequencing reaction chamber or a sequencing chip, and the The sequencing reagent is introduced into the sequencing reaction chamber or the sequencing chip, and a sequencing reaction occurs with the sample on the solid-phase carrier; then, the excitation light source is used to irradiate the surface of the solid-phase carrier, and the signal on the surface of the solid-phase carrier is detected and collected by the detector; finally, The collected signal is processed to obtain the sequence information of the sample.

In the prior art, a sequencing system includes a reagent transmission component, a sequencing reaction component, a graph acquisition component and an information processing component. Wherein, the sequencing reaction component includes a temperature control device and a sequencing reaction chamber (or sequencing chip). The control method of the sequencing system includes: step 1, performing a sequencing reaction, including a reagent transmission component transferring the reagent to the sequencing reaction chamber, and a temperature control device controlling the temperature of the measurement reaction chamber; step 2, the image acquisition component sequencing the sequencing reaction The signal on the reaction chamber is detected and collected; step 1 and step 2 are repeated until the sequencing reaction is completed; step 3, the information processing component processes the collected signal to obtain the sequence information of the sample. In this technical solution, when step 1 is performed, the image acquisition component is idle, and when step 2 is performed, the reagent transmission component and the sequencing reaction component are idle. On the one hand, the sequencing efficiency is low, and on the other hand, the utilization rate of the sequencing system is low.

Therefore, there is a need for a new sequencing control system capable of realizing rapid sequencing and improving the utilization rate of the sequencing system.

Contents of the invention

The purpose of the present invention is to provide a sequencing control system, aiming to solve the problems of low sequencing efficiency and low utilization rate of the prior art sequencing system.

In order to achieve the object of the invention, a sequencing control system includes: a first sequencing reaction unit, a first image acquisition unit, a second sequencing reaction unit, a second image acquisition unit and a control unit.

Wherein, the first sequencing reaction unit is used to control the sequencing reaction of the first sequencing reaction chamber; the first drawing unit is used to control the drawing component to perform signal detection and collection on the first sequencing reaction chamber to obtain the signal data; the second sequencing reaction unit is used to control the sequencing reaction of the second sequencing reaction chamber; the second drawing unit is used to control the drawing component to detect and collect signals from the second sequencing reaction chamber to obtain signals data; the control unit is used to control the first sequencing reaction unit and the first image acquisition unit to perform multiple cycles of work, and is used to control the second sequencing reaction unit and the second image acquisition unit to perform multiple cycles of work; the first The image acquisition unit and the second sequencing reaction unit work in the same period of time, and have the longest working time together.

Wherein, the first sequencing reaction unit is used to control the reagent transmission component to transfer the reagents to the first sequencing reaction chamber, and is used to control the temperature control device to heat or cool the first sequencing reaction chamber; the second sequencing reaction unit is used It is used to control the reagent transmission component to transfer the reagent to the second sequencing reaction chamber, and is used to control the temperature control device to heat or cool the second sequencing reaction chamber.

Wherein, the first drawing unit is used to control the leveling of the first sequencing reaction chamber, and is used to control the focusing of the first sequencing reaction chamber by the drawing assembly; the second drawing unit is used to control the second sequencing reaction Chamber leveling and used to control the focusing of the second sequencing reaction chamber of the image acquisition module.

In any of the above technical solutions, the system further includes an information processing unit, configured to perform information processing on the signal data to obtain sequence fragments.

Wherein, the first image acquisition unit includes a first signal acquisition module and a first data transmission module; the first signal acquisition module is used to control the image acquisition component to perform signal detection and collection on the first sequencing reaction chamber to obtain a signal Data; the first data transmission module is used to transmit signal data to the information processing unit in real time.

Wherein, the second image acquisition unit includes a second signal acquisition module and a second data transmission module; the second signal acquisition module is used to control the image acquisition component to perform signal detection and collection on the second sequencing reaction chamber to obtain a signal Data; the second data transmission module is used to transmit signal data to the information processing unit in real time.

In order to better achieve the purpose of the invention, the present invention also includes a sequencing control method, which includes the following steps: Step A. The first sequencing reaction unit controls the sequencing reaction of the first sequencing reaction chamber; Step B. The first drawing unit Controlling the image acquisition component to detect and collect signals from the first sequencing reaction chamber to obtain signal data; Step C. The second sequencing reaction unit controls the sequencing reaction of the second sequencing reaction chamber; Step D. The second image acquisition unit controls the image acquisition component Perform signal detection and collection on the second sequencing reaction chamber to obtain signal data; step E. repeat step A once; step F. repeat steps B to E until the first sequencing reaction chamber completes all sequencing reactions; step G. repeat steps B and step C once; step H. Repeat step D once; said step B and step C are executed within the same time period, and have the longest common execution time; said step D and step E are executed within the same time period , has the longest common execution time.

It can be seen from the above that the sequencing system of the present invention can realize parallel non-interfering sequencing control of multiple sequencing reaction chambers, realize rapid sequencing of the sequencing system, and increase the utilization rate of the sequencing system while improving the sequencing efficiency.

Description of drawings

Fig. 1 is a schematic structural diagram of a sequencing system in an embodiment of the present invention.

Fig. 2 is a schematic structural diagram of a sequencing control system in an embodiment of the present invention.

Figure 3 is a flowchart of the sequencing process in one embodiment of the present invention.

Fig. 4 is a flow chart of the sequencing reaction chamber in one embodiment of the present invention.

Fig. 5 is a diagram of processing results of an information processing unit in an embodiment of the present invention.

Detailed ways

In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

In order to make the following examples easier to understand, the present invention first provides a sequencing method. In the existing sequencing technology, a sequencing method is: using the ligation sequencing method to determine the base sequence in each sequence. Four-color fluorescent or two-color fluorescent-labeled primers are connected to sequencing anchor primers. The color of the fluorescent label is related to the base type at a specific position on the primer. The sequencing anchor primers are complementary to the known sequences on the sequencing library containing the sequence to be tested. Pairing; after each connection reaction, use excitation light to irradiate, the point with fluorescent label will be excited, and emit excitation light, and then obtain the signal of each luminescence, after analysis, it can be known that the light signal corresponds to the primer on the The bases of the sequence to be tested that are complementary at a specific position. The above sequencing method is common knowledge and will not be described in detail here. For details, please refer to the Chinese invention patent application: 201110222894.5.

For the sequencing system, the present invention proposes the first embodiment. As shown in FIG. 1 , the sequencing system may include: a sequencing reaction chamber 01 , a reagent transport component 02 , a control component 03 and a graph acquisition component 04 .

(1) Sequencing reaction chamber 01 is used for sequencing reactions.

(2) The reagent transmission component 02 is used to transmit the reagents to the sequencing reaction chamber 01 according to the instruction of the control component 04 .

(3) The control component 03 is used to control the reagent transmission component 02 and the image acquisition component 04 to work.

(4) The image acquisition component 04 is used to acquire images of the sequencing reaction chamber 01 and according to the instruction of the control component 03 .

The aforementioned sequencing reaction chamber 01 is any device or carrier that can be used to fix samples, such as a sequencing reaction chamber, a gene chip, etc., and the sequencing system includes at least one sequencing reaction chamber 01 . Preferably, the sequencing system includes two sequencing reaction chambers 01, respectively referred to as the first sequencing reaction chamber and the second sequencing reaction chamber. The reagent transport assembly 02 may include: a porous plate, a pump and a valve, the pump and the valve are connected through a conduit, the valve is located at any position above the porous plate, and the pump and the valve are used in conjunction to extract the reagents in the porous plate to the sequencing in the reaction chamber. The reagent transport assembly 02 may also include: a porous plate, a pump and a manipulator, the manipulator is connected to the pump through a conduit for extracting the reagent contained in the porous plate, and the pump may also be connected to the porous plate through a conduit to extract the porous plate Reagents contained within. The control component 03 may include: an upper computer and a lower controller. Among them, the upper computer and the lower controller can communicate through the serial port. The upper computer is used to provide an input instruction interface, and may be a computer, an embedded control panel, or the like. The lower controller is used to control the work of the reagent transmission component 02 and the image acquisition component 04 according to the instructions of the upper computer, which may be a single-chip microcomputer or a PLC. The image acquisition component 04 may include an amplifying device for amplifying the image in the spotting area of the sequencing reaction chamber and a photographing device for taking pictures of the sequencing reaction chamber through the amplifying device, wherein the amplifying device may be A microscope, a lens, etc., and the image-taking device may be a CCD, CMOS, or the like. The sequencing system of the present invention is not limited to the sequencing system in this embodiment, and the sequencing system in this embodiment is only a preferred embodiment.

For the sequencing control system used to control the sequencing system, the present invention proposes a second embodiment, as shown in FIG. 2 . The sequencing control system includes: a first sequencing reaction unit 2 , a first mapping unit 1 , a second sequencing reaction unit 3 , a second mapping unit 4 and a control unit 5 . Among them: (1) The first sequencing reaction unit 2 is used to control the sequencing reaction of the first sequencing reaction chamber. (2) The first image acquisition unit 1 is used to control the image acquisition component to detect and collect signals from the first sequencing reaction chamber to obtain signal data. (3) The second sequencing reaction unit 3 is used for controlling the sequencing reaction in the second sequencing reaction chamber. (4) The second image acquisition unit 4 is used to control the image acquisition component to detect and collect signals from the second sequencing reaction chamber to obtain signal data. (5) The control unit 5 is used to control the first sequencing reaction unit 2 and the first image acquisition unit 1 to perform multiple cycles, and to control the second sequencing reaction unit 3 and the second image acquisition unit 4 to perform multiple cycles. Preferably, the first mapping unit and the second sequencing reaction unit work within the same time period and have the longest working time together. The technical solution realizes that the sequencing system can realize rapid sequencing under the control of the sequencing control system, and improves the utilization rate of the sequencing system.

In this embodiment, the first sequencing reaction chamber and the second sequencing reaction chamber can perform sequencing reactions at the same time or at different times, and the sequencing reaction chambers after the sequencing reaction are subjected to signal detection and collection (that is, image acquisition) by the image acquisition component respectively. As shown in FIG. 2 , the control unit 5 controls the first sequencing reaction unit 2 , the second sequencing reaction unit 3 , the first image acquisition unit 1 and the second image acquisition unit 4 to work. The time for signal detection and collection is related to the number of images that the image acquisition component needs to collect for the sequencing reaction chamber. The more images collected, the longer the time for signal detection and collection; the time for the sequencing reaction is related to the sequencing method. Different sequencing reactions require different sequencing reaction times. Therefore, this embodiment provides different implementation schemes.

Preferred option 1, the time for signal detection and collection is longer than the time for sequencing reaction, as shown in 3-1 in Fig. 3 . First, the control unit 5 sends an instruction to the first sequencing reaction chamber to perform the sequencing reaction, and the first sequencing reaction unit 2 controls the first sequencing reaction chamber to perform the sequencing reaction according to the instruction, and at the same time, the control unit 5 detects the current state of the first sequencing reaction unit 2 in real time. state. After the first sequencing reaction is completed, the control unit 5 sends an image acquisition instruction to the first image acquisition unit 1, and the first image acquisition unit 1 controls the image acquisition component to perform signal detection and collection on the first sequencing reaction chamber according to the image acquisition instruction, At the same time, the control unit 5 sends an instruction for the second sequencing reaction chamber to perform the sequencing reaction, and the second sequencing reaction unit 3 controls the second sequencing reaction chamber to perform the sequencing reaction according to the instruction, and the control unit 5 detects the first drawing unit 1 and the second sequencing reaction chamber in real time. Status of Sequencing Reaction Unit 3. After the control unit 5 detects that both the first image acquisition unit 1 and the second sequencing reaction unit 3 have completed the instructions (the completion of the sequencing reaction in the second sequencing reaction chamber is earlier than or at the same time as the signal detection and collection of the first sequencing reaction chamber is completed ), the control unit 5 sends an instruction to collect images for the second sequencing reaction chamber, and the second image acquisition unit 4 controls the image acquisition component to detect and collect signals for the second sequencing reaction chamber according to the instruction. At the same time, the control unit 5 Sending an instruction for the first sequencing reaction chamber to perform the sequencing reaction, the first sequencing reaction unit 2 controls the first sequencing reaction chamber to perform the sequencing reaction according to the instruction, and the control unit 5 detects in real time the second mapping unit 4 and the first sequencing reaction unit 2 Status (the completion of the sequencing reaction in the first sequencing reaction chamber is earlier than or simultaneously with the completion of signal detection and collection in the second sequencing reaction chamber). As in the above method, both the first sequencing reaction chamber and the second sequencing reaction chamber perform sequencing reaction and signal detection in cycles until the sequencing is completed.

The second preferred option, the signal detection and collection time is shorter than the sequencing reaction time, as shown in 3-2 in Figure 3 . First, the control unit 5 sends an instruction to the first sequencing reaction chamber to perform the sequencing reaction, and the first sequencing reaction unit 2 controls the first sequencing reaction chamber to perform the sequencing reaction according to the instruction, and at the same time, the control unit 5 detects the current state of the first sequencing reaction unit 2 in real time. state. After the first sequencing reaction is completed, the control unit sends a sequencing reaction instruction for the second sequencing reaction chamber, and the second sequencing reaction unit 3 controls the second sequencing reaction chamber to perform the sequencing reaction according to the instruction, and at the same time, the control unit 5 sends an image acquisition instruction For the first image acquisition unit 1, the first image acquisition unit 1 controls the image acquisition component to detect and collect signals from the first sequencing reaction chamber according to the instruction, and the control unit 5 detects the first image acquisition unit 1 and the second sequencing reaction unit in real time 3 status. After the control unit 5 detects that both the first image acquisition unit 1 and the second sequencing reaction unit 3 have completed the instructions (the completion time of the sequencing reaction in the second sequencing reaction chamber is later than or at the same time as the completion of signal detection and collection in the first sequencing reaction chamber ), the control unit 5 sends an instruction to perform a sequencing reaction on the first sequencing reaction chamber, the first sequencing reaction unit 2 controls the first sequencing reaction chamber to perform a sequencing reaction according to the instruction, and at the same time, the control unit 5 sends a second sequencing reaction The second drawing unit 4 controls the drawing component to perform signal detection and collection on the second sequencing reaction chamber according to the instruction for the drawing of the small chamber (the completion time of the sequencing reaction of the first sequencing reaction chamber is later than or at the same time as that of the second sequencing reaction chamber) The signal detection and collection of the sequencing reaction chamber are completed as described above). Both the first sequencing reaction chamber and the second sequencing reaction chamber perform sequencing reaction and signal detection in cycles until the sequencing is completed.

The third preferred option is to carry out the sequencing reaction at the same time, and take pictures in sequence after the sequencing reaction is completed, the specific process is 3-3 in Fig. 3 . The specific control process is as follows: first, the control unit 5 sends an instruction to the sequencing reaction chamber to perform the sequencing reaction, and the first sequencing reaction unit 2 and the second sequencing reaction unit 3 work simultaneously according to the instruction, respectively controlling the sequencing reaction of the first sequencing reaction chamber and the sequencing reaction in the second sequencing reaction chamber. The control unit 5 monitors the working status of the first sequencing reaction unit 2 and the second sequencing reaction unit 3 in real time, and when it is detected that the first sequencing reaction unit 2 and the second sequencing reaction unit 3 are completed, the control unit 5 sends a response to the first sequencing reaction The first image acquisition unit 1 controls the image acquisition component to perform signal detection and collection on the first sequencing reaction chamber according to the instruction of the chamber for image acquisition. The control unit 5 monitors the working status of the first map collection unit 1 in real time. When the first sequencing map collection unit 1 is detected to be completed, the control unit 5 sends an instruction for the second sequencing reaction chamber to collect pictures, and the second map collection unit 4 According to the instruction, the image acquisition component is controlled to perform signal detection and collection on the second sequencing reaction chamber.

The optimal scheme corresponding to the above-mentioned Figure 3 can realize rapid sequencing and greatly improve the utilization rate of the sequencing system, that is, each component of the sequencing system works together to realize parallel sequencing of the first sequencing reaction chamber and the second sequencing reaction chamber. Compared with the prior art scheme, which sequentially performs the sequencing reaction-signal detection and collection of the first sequencing reaction chamber, and then performs the sequencing reaction-signal detection and collection of the second sequencing reaction chamber, the image acquisition component in the technical scheme of the present invention When taking pictures of one of the sequencing reaction chambers, the reagent transmission component, the temperature control device and the other sequencing reaction chamber jointly realize the sequencing reaction, realizing the uninterrupted work of the hardware of the sequencing system during sequencing, improving the utilization rate of the system, and improving The sequencing throughput per unit time.

In this embodiment, the workflow of each sequencing reaction chamber is shown in FIG. 4 . First, the sequencing reaction is performed. After the sequencing reaction is completed, the signal detection and collection are performed, and the sequencing is performed in cycles until the detection of the sample is completed. The control unit 5 controls the work of the sequencing system according to the instructions input through the control components. In the above-mentioned embodiment, the time of signal detection and collection can be adjusted according to needs. When it is necessary to quickly complete the picture collection, the number of pictures taken by each picture collection component or the time command is input through the control component, and the control unit 5 analyzes the input command. , according to the command to control the number of pictures taken by the picture collection component or the time, so as to realize the rapid completion of picture collection. In this technical solution, since the sequencing reaction time is generally different from the image acquisition time, when the image acquisition time is far from the sequencing reaction time, the image acquisition time can be changed by setting the number of images acquired by each image acquisition component, and the acquisition time can be reduced. The gap between the mapping time and the sequencing reaction time can realize the uninterrupted progress of the sequencing reaction and the mapping action, and improve the efficiency of sequencing. In addition, the control unit 5 uses multithreading to control the working order of the first sequencing reaction chamber and the second sequencing reaction chamber, so that the first sequencing reaction chamber and the second sequencing reaction chamber can work in parallel without interfering with each other, greatly improving the sequencing system. work efficiency.

The first sequencing reaction unit in the second embodiment is used to control the sequencing reaction of the first sequencing reaction chamber. After the first sequencing reaction unit receives the reagent transfer instruction from the control unit, it controls the reagent transfer component to transfer the reagent to the first sequencing reaction chamber; after the first sequencing reaction unit receives the temperature instruction from the control unit, it controls the temperature control device to The sequencing reaction chamber is heated or refrigerated. Under the temperature control of the temperature control device, the reagents in the first sequencing reaction chamber perform the sequencing reaction. After the second sequencing reaction unit receives the reagent transmission instruction from the control unit, it controls the reagent transmission component to transfer the reagent to the second sequencing reaction chamber; after the second sequencing reaction unit receives the temperature instruction from the control unit, it controls the temperature control device to The second sequencing reaction chamber is heated or cooled. Under the temperature control of the temperature control device, the reagents in the second sequencing reaction chamber undergo a sequencing reaction. Different sequencing reaction conditions corresponding to different sequencing methods during sequencing, the sequencing reaction conditions include: the type and dosage of reagents in the sequencing reaction chamber, the temperature of the sequencing reaction, and the reaction time at each temperature. It is worth noting that the reagent transmission instruction and the temperature instruction in the present invention have no sequence, that is to say, the reagent transmission component transmits the reagent first, and then the temperature control device controls the temperature; or the temperature control device controls the temperature while the reagent transmission component transmits the reagent. ; or the temperature control device first controls the temperature of the sequencing reaction chamber, and the reagent transmission component is performing reagent transmission. Preferably, the temperature control device controls the temperature while the reagent transport component is transporting the reagent, which can greatly improve the efficiency of the sequencing reaction without waiting for reagent transport or temperature control alone.

After the first sequencing reaction chamber completes the sequencing reaction, firstly, the first image acquisition unit controls the leveling of the first sequencing reaction chamber, that is, the plane where the area where the sample is fixed in the first sequencing reaction chamber is located is perpendicular to the image acquisition component; then , the first image acquisition component controls the image acquisition component to focus on the first sequencing reaction chamber, and at the same time controls the exposure of the image acquisition component, that is, realizes signal detection; then, the image acquisition component collects images on the first sequencing reaction chamber, that is Implement signal collection. The control mode of the second image acquisition unit is the same as that of the first image acquisition unit, and will not be repeated here. Wherein, the leveling of the sequencing reaction chamber may be performed once after each sequencing reaction is completed, or may be performed only once during each sequencing process after each sequencing reaction chamber completes the sequencing reaction for the first time, or That is, each sequencing reaction chamber is only leveled once during the entire sequencing process. In this technical solution, the sequencing system is under the control of the first and second image acquisition units, through operations such as automatic leveling, focusing, and exposure, so that the image acquisition component can collect accurate data in the sequencing reaction chamber, thereby ensuring the sequencing The accuracy of the results also ensures the throughput of sequencing.

The sequencing control system in the second embodiment may further include an information processing unit, configured to perform information processing on the signal data to obtain sequence fragments. The information processing unit first identifies the signal data, for example, according to the magnitude of the signal strength. 5-1 in FIG. 5 is the signal data obtained through the signal detection and collection of the image collection component in the present invention, and each bright spot represents a base signal of a color. When sequencing uses four-color fluorescent probes to mark bases, the image acquisition component can detect and collect signals four times at the same position to obtain four signal data corresponding to the same base at the same position; when sequencing uses two-color fluorescent probes When the needle marks the base, when the same base is detected at the same position, two sequencing reactions need to be performed. After each sequencing reaction, the image acquisition component performs two signal detection and collection on the same position to obtain the same base at the same position. The base corresponds to the four signal data. The following is an example of the analysis results of bases labeled with four-color fluorescent probes. The information processing unit first conducts a preliminary identification of the signal data on the same base, that is, counts the signal strengths on the four pictures taken. 5-2 in FIG. 5 is the statistical signal data, and the information processing unit identifies the type of the base according to the signal data in 5-2. As can be seen from 5-2 in Figure 5, the base corresponding to base1 is A, the base corresponding to base2 is T, the base corresponding to base3 is G, the base corresponding to base4 is A, and the base corresponding to base5 is C. This method can obtain the bases corresponding to all signal data.

In the second embodiment, the first image acquisition unit includes a first signal acquisition module and a first data transmission module. Wherein, the first signal acquisition module is used to control the image acquisition component to detect and collect signals from the first sequencing reaction chamber to obtain signal data; the first data transmission module is used to transmit signal data to the information processing unit. The signal detection and collection process of the first sequencing reaction chamber by the imaging component is as follows: First, the first signal acquisition module controls the imaging component to focus and expose the first sequencing reaction chamber, wherein the focusing method includes but not limited to: "Z-axis drift correction system" or "based on image wavelet transform and defined as a function of high-pass and low-pass band", the exposure method has no special restrictions; then, the first signal acquisition module controls the image acquisition component to the second A sequencing reaction chamber carries out signal collection, that is, image acquisition, to obtain signal data; then, the first data transmission module transmits the signal data in the image acquisition component to the information processing unit; finally, the information processing unit processes the signal data to obtain base sequence. Wherein, the first data transmission module can transmit the signal data in real time, or can transmit the signal data to the information processing unit after the image acquisition component completes the signal collection of the first sequencing reaction chamber. The second image acquisition unit includes a second signal acquisition module and a second data transmission module. The second signal acquisition module is used to control the image acquisition component to detect and collect signals from the second sequencing reaction chamber to obtain signal data; the second data transmission module is used to transmit signal data to the information processing unit. The working mode of the second image acquisition unit is the same as that of the first image acquisition unit, and reference may be made to the first image acquisition unit, which will not be repeated here.

The present invention proposes a third embodiment, a sequencing control method based on the above sequencing control system. The sequencing control method includes the following steps.

Step A. The first sequencing reaction unit controls the sequencing reaction of the first sequencing reaction chamber. Step B. The first image acquisition unit controls the image acquisition component to detect and collect signals from the first sequencing reaction chamber to obtain signal data. Step C. The second sequencing reaction unit controls the sequencing reaction of the second sequencing reaction chamber. Step D. The second image acquisition unit controls the image acquisition component to detect and collect signals from the second sequencing reaction chamber to obtain signal data. Step E. Repeat Step A once. Step F. Repeat steps B to E until the first sequencing reaction chamber completes all the sequencing reactions. Step G. Repeat steps B and C once. Step H. Repeat Step D once.

In this embodiment, the steps A to H may not be executed sequentially or may be executed sequentially. Preferably, the step B and step C are executed within the same time period and have the longest common execution time; the step D and step E are executed within the same time period and have the longest common execution time. The specific flow of the preferred solution is shown in FIG. 3 , which will not be repeated here.

In this embodiment, the step A includes: A1. The reagent transport component of the first sequencing reaction unit transfers the reagents to the first sequencing reaction chamber; A2. The first sequencing reaction unit controls the temperature control device to heat the first sequencing reaction chamber or refrigeration. Preferably, the steps A1 and A2 are in no sequence.

The step C includes: C1. The reagent transmission component of the second sequencing reaction unit transmits the reagent to the second sequencing reaction chamber; C2. The second sequencing reaction unit controls the temperature control device to heat or cool the second sequencing reaction chamber; Step C1 and Step C2 are in no order.

Preferably, the step A1 and the step A2 are executed sequentially; the step C1 and the step C2 are executed sequentially.

Preferably, the step B and step C start to be executed or end at the same time; the step D or step E starts to be executed at the same time or end at the same time.

Wherein, the step B includes: B1. The first sequencing reaction chamber is controlled by the first drawing unit to level it; B2. The first drawing unit controls the drawing assembly to focus on the first sequencing reaction chamber; B3. Drawing The component performs signal detection and collection on the first sequencing reaction chamber to obtain signal data.

Wherein, the step D includes: D1. The second sequencing reaction chamber is controlled by the second image acquisition unit to level it; D2. The second image acquisition unit controls the image acquisition component to focus on the second sequencing reaction chamber; D3. The component performs signal detection and collection on the second sequencing reaction chamber to obtain signal data.

The sequencing control system also includes an information processing unit, which is used to perform I. Carry out information processing on the signal data to obtain sequence fragments; the step I is located after the step B and the step D at the same time, or only after the step H; the step Steps B and D also include real-time transmission of signal data.

Existing second-generation high-throughput sequencing methods can be sequenced using the technical solution of the present invention, including but not limited to ligation sequencing and synthesis sequencing.

It should be noted that the typical application of the present invention is not limited to the field of automation control, and the control system described in the present invention can also be applied in other similar fields.

In addition, the above-mentioned control system is not only for the sequencing system in the embodiment of the present invention, the control system of the present invention can be used in the sequencing system with two sequencing reaction chambers in the prior art.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be included in the protection of the present invention. within range.

Claims (6)

1. A sequencing control system, the sequencing control system is used to control the sequencing system; it is characterized in that, The sequencing system includes two sequencing reaction chambers, a reagent transport assembly, a control assembly and a graph acquisition assembly; The sequencing reaction chambers are used for sequencing reactions and are respectively referred to as the first sequencing reaction chamber and the second sequencing reaction chamber; The reagent transmission component is used to transmit the reagent to the sequencing reaction chamber according to the instruction of the control component; The control component is used to control the reagent transmission component and the image acquisition component to work; The image acquisition component is used to acquire images of the sequencing reaction chamber according to the instructions of the control component; The sequencing control system includes a first sequencing reaction unit, a first drawing unit, a second sequencing reaction unit, a second drawing unit and a control unit; The first sequencing reaction unit is used to control the sequencing reaction of the first sequencing reaction chamber; The first image acquisition unit is used to control the image acquisition component to detect and collect signals from the first sequencing reaction chamber to obtain signal data; The second sequencing reaction unit is used to control the sequencing reaction of the second sequencing reaction chamber; The second image acquisition unit is used to control the image acquisition component to detect and collect signals from the second sequencing reaction chamber to obtain signal data; The control unit is used to control the first sequencing reaction unit and the first image acquisition unit to perform multiple cycles, and is used to control the second sequencing reaction unit and the second image acquisition unit to perform multiple cycle operations; The first image acquisition unit and the second sequencing reaction unit work in the same period of time, and have the longest working time together. 2. The sequencing control system according to claim 1, wherein the first sequencing reaction unit is used to control the reagent transfer component to transfer the reagents to the first sequencing reaction chamber, and is used to control the temperature control device to perform the first sequencing reaction. The reaction chamber is heated or cooled; The second sequencing reaction unit is used to control the reagent transport component to transfer the reagents to the second sequencing reaction chamber, and is used to control the temperature control device to heat or cool the second sequencing reaction chamber. 3. The sequencing control system according to claim 1, wherein the first drawing unit is used to control the leveling of the first sequencing reaction chamber, and is used to control the focusing of the first sequencing reaction chamber by the drawing assembly ; The second image collection unit is used to control the leveling of the second sequencing reaction chamber, and to control the focusing of the second sequencing reaction chamber of the image collection component. 4. The sequencing control system according to any one of claims 1 to 3, characterized in that the sequencing control system further comprises an information processing unit, configured to perform information processing on signal data to obtain sequence fragments. 5. The sequencing control system according to claim 4, wherein the first image acquisition unit comprises a first signal acquisition module and a first data transmission module; The first signal acquisition module is used to control the image acquisition component to detect and collect signals from the first sequencing reaction chamber to obtain signal data; The first data transmission module is configured to transmit signal data to the information processing unit in real time. 6. The sequencing control system according to claim 4, wherein the second image acquisition unit comprises a second signal acquisition module and a second data transmission module; The second signal acquisition module is used to control the image acquisition component to detect and collect signals from the second sequencing reaction chamber to obtain signal data; The second data transmission module is configured to transmit signal data to the information processing unit in real time.