CN103007343B - Hard tissue repair and substitute material and preparation method thereof - Google Patents
Hard tissue repair and substitute material and preparation method thereof Download PDFInfo
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- CN103007343B CN103007343B CN201310015917.4A CN201310015917A CN103007343B CN 103007343 B CN103007343 B CN 103007343B CN 201310015917 A CN201310015917 A CN 201310015917A CN 103007343 B CN103007343 B CN 103007343B
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- hard tissue
- tissue repair
- preparation
- temperature
- replacement material
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- 239000000463 material Substances 0.000 title claims abstract description 34
- 230000017423 tissue regeneration Effects 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- ZQBZAOZWBKABNC-UHFFFAOYSA-N [P].[Ca] Chemical compound [P].[Ca] ZQBZAOZWBKABNC-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000002253 acid Substances 0.000 claims abstract description 10
- 239000000919 ceramic Substances 0.000 claims abstract 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 22
- 229910052757 nitrogen Inorganic materials 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 5
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 claims description 4
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 4
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 4
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 4
- 239000004472 Lysine Substances 0.000 claims description 4
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 4
- 235000004279 alanine Nutrition 0.000 claims description 4
- 239000004068 calcium phosphate ceramic Substances 0.000 claims description 4
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 claims description 4
- 229960002591 hydroxyproline Drugs 0.000 claims description 4
- 239000000178 monomer Substances 0.000 claims description 4
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims description 4
- 239000000376 reactant Substances 0.000 claims description 4
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 230000002051 biphasic effect Effects 0.000 claims description 2
- SLXKOJJOQWFEFD-UHFFFAOYSA-N 6-aminohexanoic acid Chemical compound NCCCCCC(O)=O SLXKOJJOQWFEFD-UHFFFAOYSA-N 0.000 claims 1
- 229960002684 aminocaproic acid Drugs 0.000 claims 1
- 230000018044 dehydration Effects 0.000 claims 1
- 238000006297 dehydration reaction Methods 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 238000006116 polymerization reaction Methods 0.000 claims 1
- 210000000988 bone and bone Anatomy 0.000 abstract description 7
- 239000012620 biological material Substances 0.000 abstract description 4
- 230000004071 biological effect Effects 0.000 abstract description 2
- 229920001308 poly(aminoacid) Polymers 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 description 22
- 238000010792 warming Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 4
- 239000001506 calcium phosphate Substances 0.000 description 3
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 3
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 3
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 229910000391 tricalcium phosphate Inorganic materials 0.000 description 2
- 229940078499 tricalcium phosphate Drugs 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000002138 osteoinductive effect Effects 0.000 description 1
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Abstract
本发明属于生物材料技术领域，提供了一种硬组织修复及替代材料及其制备方法，该硬组织修复及替代材料由添加了聚氨基酸（PAA）的双相钙磷陶瓷（BCP）构成。通过添加聚氨基酸改良双相钙磷陶瓷的力学强度，获得了兼具良好生物学活性和足够力学强度的硬组织修复及替代材料，解决了单一双相钙磷陶瓷的力学强度较低，难以作为骨修复和替代材料起到结构性支撑的作用而临床应用受到极大制约的问题，该制备方法简单，成本较低，实用性强，具有较强的推广与应用价值。
The invention belongs to the technical field of biomaterials, and provides a hard tissue repair and replacement material and a preparation method thereof. The hard tissue repair and replacement material is composed of dual-phase calcium-phosphorus ceramics (BCP) added with polyamino acid (PAA). By adding polyamino acids to improve the mechanical strength of dual-phase calcium-phosphorus ceramics, a hard tissue repair and replacement material with good biological activity and sufficient mechanical strength has been obtained, which solves the problem that the mechanical strength of single-phase dual-phase calcium-phosphorus ceramics is low and difficult to be used as Bone repair and replacement materials play the role of structural support, but clinical application is greatly restricted. The preparation method is simple, low in cost, strong in practicability, and has strong promotion and application value.
Description
Technical field
The invention belongs to technical field of biological materials, relate in particular to a kind of hard tissue repair and substitution material and preparation method thereof.
Background technology
At present hard tissue repair and alternative biomaterial are various in style, but can successfully apply to clinical seldom, main cause is to make the biological activity of biomaterial and mechanical strength reach good balance, particularly on mechanical strength, does not often reach the requirement of clinical practice.Biphase calcium phosphor pottery (biphasic calcium phosphate, BCP) bioactive materials being formed by hydroxyapatite and bata-tricalcium phosphate, combine hydroxyapatite (hydroxyapatite, HA) high strength, high bioactivity, stability and bata-tricalcium phosphate (β-tricalcium phosphate, β-TCP) absorption degradation characteristic, BCP has obtained a large amount of further investigations and extensive use for a long time as bone renovating material.BCP has good osteoinductive and bone conductibility, can be processed into loose structure, and can regulate by loose structure degradation property and the mechanical strength of material.But the mechanical strength of single biphase calcium phosphor pottery (BCP) is lower, only 0.5 ~ 5MPa, is difficult to play as bone reparation and substitution material the effect of structural support, makes its clinical practice be subject to very big restriction.
Summary of the invention
The invention provides a kind of hard tissue repair and substitution material and preparation method thereof, the mechanical strength that is intended to solve single biphase calcium phosphor pottery is lower, is difficult to play the effect of structural support and clinical practice is subject to the greatly problem of restriction as bone reparation and substitution material.
The object of the present invention is to provide a kind of hard tissue repair and substitution material, this hard tissue repair and substitution material are made up of the biphase calcium phosphor pottery (BCP) that has added polyamino acid (PAA).
Another object of the present invention is to provide the preparation method of a kind of hard tissue repair and substitution material, this preparation method comprises the following steps:
Step 1, joins 63.16g 6-aminocaprolc acid, 2.76g proline, 2.68g alanine, 2.96g phenylalanine, 1.8g lysine, 3.00g hydroxyproline and 100ml water in the three-necked bottle of 250ml with agitating device and nitrogen protection device;
Step 2, opens nitrogen protection and stirring, is progressively warming up to 200 DEG C, is warming up to 210 DEG C after having dewatered, and reactant is all melt into bright orange transparent melt at this temperature;
Step 3; after molten monomer, be warming up to 220 DEG C and start polyreaction 3 hours; and to add 49.89g content be 40% biophasic calcium phosphate ceramic, be warming up to 230 DEG C of reactions 3 hours, after reaction finishes, under nitrogen protection, cool to room temperature obtains block hard tissue repair and substitution material.
Hard tissue repair provided by the invention and substitution material and preparation method thereof, by adding the mechanical strength of polyamino acid improvement biphase calcium phosphor pottery, the hard tissue repair and the substitution material that have the active and enough mechanical strengths of good biological concurrently are obtained, the mechanical strength that has solved single biphase calcium phosphor pottery is lower, be difficult to play the effect of structural support and clinical practice is subject to the greatly problem of restriction as bone reparation and substitution material, this preparation method is simple, cost is lower, practical, there is stronger propagation and employment and be worth.
Brief description of the drawings
Fig. 1 is the realization flow figure of the preparation method of the hard tissue repair that provides of the embodiment of the present invention and substitution material.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is described in further detail.Should be appreciated that specific embodiment described herein is only in order to explain the present invention, and be not used in restriction invention.
The object of the present invention is to provide a kind of hard tissue repair and substitution material, this hard tissue repair and substitution material are made up of the biphase calcium phosphor pottery (BCP) that has added polyamino acid (PAA).
Fig. 1 shows the realization flow of the preparation method of hard tissue repair that the embodiment of the present invention provides and substitution material.
This preparation method comprises the following steps:
In step S101,63.16g 6-aminocaprolc acid, 2.76g proline, 2.68g alanine, 2.96g phenylalanine, 1.8g lysine, 3.00g hydroxyproline and 100ml water are joined in the three-necked bottle of 250ml with agitating device and nitrogen protection device;
In step S102, open nitrogen protection and stirring, be progressively warming up to 200 DEG C, after having dewatered, be warming up to 210 DEG C, reactant is all melt into bright orange transparent melt at this temperature;
In step S103; after molten monomer, be warming up to 220 DEG C and start polyreaction 3 hours; and to add 49.89g content be 40% biophasic calcium phosphate ceramic, be warming up to 230 DEG C of reactions 3 hours, after reaction finishes, under nitrogen protection, cool to room temperature obtains block hard tissue repair and substitution material.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
The present invention intends the mechanical strength by adding polyamino acid (PAA) improvement biphase calcium phosphor pottery, becomes the hard tissue repair and the substitution material that have the active and enough mechanical strengths of good biological concurrently
Fig. 1 shows the realization flow of the preparation method of hard tissue repair that the embodiment of the present invention provides and substitution material; by 63.16g 6-aminocaprolc acid; 2.76g proline; 2.68g alanine; 2.96g phenylalanine; 1.8g lysine; 3.00g hydroxyproline and 100ml water join in the three-necked bottle of 250ml with agitating device and nitrogen protection device; open nitrogen protection and stirring; progressively be warming up to 200 DEG C; after having dewatered, be warming up to 210 DEG C, all meltings of reactant at this temperature, become bright orange transparent melt.After molten monomer, be warming up to 220 DEG C and start polyreaction 3h.Now, add 49.89g content to be about the biophasic calcium phosphate ceramic of 40 %, be warming up to 230 DEG C of reaction 3h, after reaction finishes, under nitrogen protection, cool to room temperature obtains block composite.
The hard tissue repair that the embodiment of the present invention provides and substitution material and preparation method thereof, by adding the mechanical strength of polyamino acid improvement biphase calcium phosphor pottery, the hard tissue repair and the substitution material that have the active and enough mechanical strengths of good biological concurrently are obtained, the mechanical strength that has solved single biphase calcium phosphor pottery is lower, be difficult to play the effect of structural support and clinical practice is subject to the greatly problem of restriction as bone reparation and substitution material, this preparation method is simple, cost is lower, practical, there is stronger propagation and employment and be worth.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
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| CN201310015917.4A CN103007343B (en) | 2013-01-16 | 2013-01-16 | Hard tissue repair and substitute material and preparation method thereof |
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| CN103007343B true CN103007343B (en) | 2014-06-25 |
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| CN105288748B (en) * | 2015-12-03 | 2018-01-16 | 广东省微生物研究所 | A kind of anti-infective calcium phosphate composite bone cement material and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080102097A1 (en) * | 2006-10-31 | 2008-05-01 | Zanella John M | Device and method for treating osteolysis using a drug depot to deliver an anti-inflammatory agent |
| CN101342384A (en) * | 2008-08-25 | 2009-01-14 | 四川国纳科技有限公司 | Composite polymer bone-renovation material containing ceramic component and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080102097A1 (en) * | 2006-10-31 | 2008-05-01 | Zanella John M | Device and method for treating osteolysis using a drug depot to deliver an anti-inflammatory agent |
| CN101342384A (en) * | 2008-08-25 | 2009-01-14 | 四川国纳科技有限公司 | Composite polymer bone-renovation material containing ceramic component and preparation method thereof |
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