CN102987165A - South Asia trypetid larva artificial feed - Google Patents
South Asia trypetid larva artificial feed Download PDFInfo
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- CN102987165A CN102987165A CN2012105771374A CN201210577137A CN102987165A CN 102987165 A CN102987165 A CN 102987165A CN 2012105771374 A CN2012105771374 A CN 2012105771374A CN 201210577137 A CN201210577137 A CN 201210577137A CN 102987165 A CN102987165 A CN 102987165A
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- trypetid
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims abstract description 11
- 235000010234 sodium benzoate Nutrition 0.000 claims abstract description 11
- 239000004299 sodium benzoate Substances 0.000 claims abstract description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 8
- 229930006000 Sucrose Natural products 0.000 claims abstract description 8
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 241000976737 Zeugodacus tau Species 0.000 claims description 20
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 claims description 10
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 claims description 10
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 7
- 241000238631 Hexapoda Species 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 abstract description 2
- 231100000027 toxicology Toxicity 0.000 abstract description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 abstract 1
- 230000003449 preventive effect Effects 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- 102000002322 Egg Proteins Human genes 0.000 description 13
- 108010000912 Egg Proteins Proteins 0.000 description 13
- 210000004681 ovum Anatomy 0.000 description 13
- 241000382353 Pupa Species 0.000 description 12
- 241000255925 Diptera Species 0.000 description 7
- 230000032669 eclosion Effects 0.000 description 7
- 235000013601 eggs Nutrition 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 229920003023 plastic Polymers 0.000 description 6
- 239000004033 plastic Substances 0.000 description 6
- 239000004576 sand Substances 0.000 description 5
- 240000001980 Cucurbita pepo Species 0.000 description 4
- 244000302544 Luffa aegyptiaca Species 0.000 description 4
- 235000009814 Luffa aegyptiaca Nutrition 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 230000012447 hatching Effects 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 235000000832 Ayote Nutrition 0.000 description 2
- 241001427559 Collembola Species 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 2
- 235000009854 Cucurbita moschata Nutrition 0.000 description 2
- 235000009852 Cucurbita pepo Nutrition 0.000 description 2
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 2
- 244000302512 Momordica charantia Species 0.000 description 2
- 235000009811 Momordica charantia Nutrition 0.000 description 2
- 235000009812 Momordica cochinchinensis Nutrition 0.000 description 2
- 235000018365 Momordica dioica Nutrition 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 235000015136 pumpkin Nutrition 0.000 description 2
- 229910000838 Al alloy Inorganic materials 0.000 description 1
- 244000189799 Asimina triloba Species 0.000 description 1
- 235000006264 Asimina triloba Nutrition 0.000 description 1
- 241001124183 Bactrocera <genus> Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 244000179684 Passiflora quadrangularis Species 0.000 description 1
- 235000011266 Passiflora quadrangularis Nutrition 0.000 description 1
- 241000508269 Psidium Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 241000255588 Tephritidae Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Fodder In General (AREA)
Abstract
The invention relates to the field of artificial feeding of insects and particularly relates to an artificial feed for South Asia trypetid larvae. The artificial feed for South Asia trypetid larvae comprises the following raw materials by weight percent: 25-30% of wheat bran, 9-11% of cane sugar, 3.5-6.0% of beer yeast, 0.1-0.2% of sodium benzoate, 0.1-0.2% of methyl parahydroxybenzoats and the balance of water. The artificial feed for South Asia trypetid larvae is low cost and wide in sources of raw materials and can meet the growth and development requirement of the South Asia trypetid larvae. Thus, lots of healthy and regularly developed insect sources for test can be supplied for the research and preventive treatment of physiology, genetics, toxicology and the like.
Description
Technical field
The present invention relates to insect artificial feeding field, be specifically related to a kind of Bactrocera tau larva artificial feed.
Background technology
Bactrocera tau
Bactrocera tau(walker) belong to Diptera Diptera, Tephritidae
Tephritidae, Bactrocera
BactroceraMacquart, can endanger the 70 various fruits vegetables such as the melon and citrus of 13 kinds of sponge gourd, pumpkin, cucumber, balsam pear, cucurbita pepos etc., mango, tomato, pawpaw guava, a kind of important fruit, vegetable-crop pest-insect, at present in China Taiwan and provinces and regions, 14 continents distribution is arranged, be on the rise in the harm of China south China, southwest, Central China.
The artificial feeding of Bactrocera tau larva still mainly adopts at present the fruit of its natural host plant such as sponge gourd, cucumber, pumpkin, balsam pear etc. to raise, but raise the restriction that Bactrocera tau is subject to host, season with natural feed, cost is high, can not satisfy the needs of every research; On the other hand, owing to using in a large number of agricultural chemicals, the control of the difficult quality of every batch natural host fruit, thereby be unable to supply the experiment unified, health of a large amount of physiological standards and use worm.
Summary of the invention
A kind of Bactrocera tau larva artificial feed provided by the present invention, cost is low, raw material sources are wide, can satisfy the needs that grow of Bactrocera tau larva.
The present invention adopts following scheme:
A kind of Bactrocera tau larva artificial feed, its raw material consists of: 25～30% wheat bran, 9～11% sucrose, 3.5～6.0% brewer's yeasts, 0.1～0.2% Sodium Benzoate, 0.1～0.2% methyl p-hydroxybenzoate, surplus are water.
More specifically, comprise 750～850g wheat bran, 250～350g sucrose, 100～200g brewer's yeast, 2.5～3.5g Sodium Benzoate, 3～4g methyl p-hydroxybenzoate and 1.5～2L water in every part of man-made feeds.
As preferably, every part of man-made feeds contain 800g wheat bran, 300g sucrose, 150g yeast, 3g Sodium Benzoate, 3.5g methyl p-hydroxybenzoate and 1.5L water.First 3g Sodium Benzoate, 3.5g methyl p-hydroxybenzoate are dissolved with a small amount of warm water, then mix with other compositions, be tiled in the larva raising dish after adding 1.5L running water is mixed thoroughly.
Beneficial effect:
The Bactrocera tau larva artificial feed that the present invention adopts such scheme to provide, cost is low, raw material sources are wide, can reduce the artificial feeding cost of Bactrocera tau, and can fully satisfy the needs that grow of Bactrocera tau larva, the research and the preventing and controlling that can be the aspects such as physiology, science of heredity, toxicology provide a large amount of health, neat the supplying of growth to try the worm source.
The specific embodiment
Embodiment 1 most preferred embodiment
The artificial feeding step of Bactrocera tau comprises that adopting ovum → ovum hatches into larva → larva artificial feeding → larvae pupation → pupa and sprout wings into adult → imago breeding etc.
One, man-made feeds preparation
Fill a prescription according to the Bactrocera tau larva artificial feed, the 800g wheat bran, 300g sucrose, the 150g beer waste yeast that weigh up are poured in larger plastic tub or the bucket, taking by weighing 3g Sodium Benzoate, 3.5g methyl p-hydroxybenzoate pours in the little container (plastic cup or glass) and to pour in the said vesse after dissolving with a small amount of warm water, add the 1.5L running water, after stirring, pour larva raising dish (plastic material into, 49 cm X32 cm X5cm), every dish is poured 2 parts of man-made feeds into, about 5.5kg, manual decurl, and at centre position layer overlay toilet paper, for subsequent use.
Two, adopt ovum, spread ovum, pupate
The Bactrocera tau Adult Development during to sexal maturity (about 20d sexal maturity behind the adult eclosion) carry out mating, lay eggs.The ovum artificial collection method is to put into the artificial feeding cage with adopting egg apparatus, take out after about 5-6 hour and adopt egg apparatus, water is gone out ovum from adopt egg apparatus, the ovum amount that arrives with the graduated cylinder measurement collection, the about 6ml ovum of every dish feed Pan Pu, ovum is tiled on the toilet paper, with the watering can watering ovum is roughly disperseed, then rearing tray is placed on the springtail frame, springtail frame below is placed with sand table, at 26 ± 1 ℃, raise under RH70 ± 10% condition, after about 1 week, mature larva can be grown in sand table from the rearing tray introskip and pupated, and behind about 4d pupa is sifted out from sand.
Three, quality-monitoring
Raised for 3 generations continuously with man-made feeds, the host who adopts Bactrocera tau to have a liking for most simultaneously----sponge gourd breeding grub compares (CK), to per generation the quality index such as egg hatching rate, survival rate of larvae, pupa weight, eclosion rate, the rate that flies out monitor.
Egg hatching rate:
Get at random 100 on each generation and CK offspring ovum, place on the moistening filter paper of culture dish, place under (25 ± 1) ℃, RH 65% ± 5%, the L:D=12:12 condition, check unhatched ovum at microscopically after 48 hours and count L, calculate egg hatching rate H%, H%=(100-L)/100 * 100.Three groups of repetitions are established in experiment.
Survival rate of larvae:
Get at random each generation and CK and just hatched 100 of larvas, place respectively in the plastics tray that man-made feeds and sponge gourd are housed, again vinyl disc is placed the bottom to be covered with in the plastic tub of fine sand.Under (25 ± 1) ℃, RH 65% ± 5%, L:D=12:12 condition, raise, jump in the sand and the rear sieve of all pupating is husky, check pupa quantity P alive, calculating survival rate of larvae S%, S%=P/100 * 100 until larva is aging.Three groups of repetitions are established in experiment.
Pupa is heavy: get at random 100 of the pupas in 10 days pupa ages, weigh, average;
Eclosion rate, rate flies out: at 1.2 m * 0.6 m * 1.2 m dependent insect cage (aluminum alloy frames, the nylon grenadine) put 5 diameter 10 ㎝ in, high 20 ㎝ inwalls scribble talcous black plastic cylinder, put into the pupa in 100 10 days pupa ages in every, place under (25 ± 1) ℃, RH 65% ± 5%, the L:D=12:12 condition, the respectively dead pupa quantity A in the recording drum, partly sprout wings quantity B, lopsided trypetid quantity C, the normal but trypetid quantity D of the cylinder of failing to fly out after 6 days are calculated respectively eclosion rate E%, the rate that flies out F%.
E%＝(100-A-B)/100×100
F%＝(100-A-B-C-D)/100×100。
Four, results and analysis
Table 1 Bactrocera tau man-made feeds are raised the quality index of raising with sponge gourd and are compared
Annotate: data are average ± standard error in the table.Adopt the Tukey multiple ratio, with different letter representation significant differences behind the column data, wherein lowercase be illustrated in 0.05 (
P＜0.05) significant difference on the level, capitalization be illustrated in 0.01 (
P＜0.01) significant difference on the level.
As can be seen from Table 1, man-made feeds are raised the incubation rate and contrast there was no significant difference of the ovum in 3 generations continuously, and all more than 90%; There is not significant difference in survival rate of larvae with contrast yet, all greater than 80%.
The pupa weight average is heavy the heaviest greater than the pupa of contrast and 1st generation, has significant difference with 2nd generation and contrast, but different not remarkable with the 3rd generation-difference; 2nd, 3 generations and contrast poor between without significant difference.
1st generation eclosion rate and the rate that flies out are the highest, and the eclosion rate in the 2nd, 3 generations, the rate that flies out all slightly are lower than contrast, but the eclosion rate in the 1st, 2,3 generations and the difference that flies out that there are no significant between rate and the contrast.
The result show Bactrocera tau quality that man-made feeds raise continuously and host's raising without significant difference, can be with this larva artificial feed mass rearing Bactrocera tau.
Embodiment 2(following examples are in order to support better claim)
Comprise 750g wheat bran, 250g sucrose, 150g brewer's yeast, 2.5g Sodium Benzoate, 3.5g methyl p-hydroxybenzoate and 1.5L water in every part of man-made feeds.
First Sodium Benzoate, methyl p-hydroxybenzoate are dissolved with a small amount of warm water, then mix with other compositions, be tiled in the larva raising dish after adding 1.5L running water is mixed thoroughly.
Embodiment 3
Comprise 850g wheat bran, 350g sucrose, 200g brewer's yeast, 3.5g Sodium Benzoate, 4g methyl p-hydroxybenzoate and 2L water in every part of man-made feeds.
First Sodium Benzoate, methyl p-hydroxybenzoate are dissolved with a small amount of warm water, then mix with other compositions, be tiled in the larva raising dish after adding 2L running water is mixed thoroughly.
Claims (1)
1. Bactrocera tau larva artificial feed, it is characterized in that: the raw material of described Bactrocera tau larva artificial feed consists of: 25～30% wheat bran, 9～11% sucrose, 3.5～6.0% brewer's yeasts, 0.1～0.2% Sodium Benzoate, 0.1～0.2% methyl p-hydroxybenzoate, surplus are water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105771374A CN102987165A (en) | 2012-12-27 | 2012-12-27 | South Asia trypetid larva artificial feed |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012105771374A CN102987165A (en) | 2012-12-27 | 2012-12-27 | South Asia trypetid larva artificial feed |
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| CN2012105771374A Pending CN102987165A (en) | 2012-12-27 | 2012-12-27 | South Asia trypetid larva artificial feed |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103300257A (en) * | 2013-07-09 | 2013-09-18 | 海南出入境检验检疫局热带植物隔离检疫中心 | Artificial feed for bactrocera dorsalis larvas |
| CN104095151A (en) * | 2013-04-02 | 2014-10-15 | 湖南师范大学 | Semi-artificial feed for Bactrocera minax larvae and preparation method thereof |
| CN104206695A (en) * | 2014-09-26 | 2014-12-17 | 张可池 | Mixed feed special for tachinid and preparation method thereof |
| CN105166585A (en) * | 2015-10-28 | 2015-12-23 | 浙江省柑桔研究所 | Artificial feed for bactrocera tau adults and preparation method thereof |
| CN106036259A (en) * | 2016-05-30 | 2016-10-26 | 中国农业科学院植物保护研究所 | Artificial feed for larval delia antiqua (Meigen) as well as preparation method of artificial feed and raising method of larval delia antiqua (Meigen) |
| CN106173568A (en) * | 2016-07-22 | 2016-12-07 | 红河学院 | A kind of Fructus Capsici trypetid larva artificial feed and preparation method thereof |
| CN106306610A (en) * | 2016-08-23 | 2017-01-11 | 张驰 | Feed for larvae of apple flies and preparation method thereof |
| CN106689825A (en) * | 2016-12-05 | 2017-05-24 | 长沙秋点兵信息科技有限公司 | High-hatchability feed for bactrocera mediterranei larvae |
| CN106689826A (en) * | 2016-12-05 | 2017-05-24 | 长沙秋点兵信息科技有限公司 | Mediterranean fruit fly larva feed capable of shortening hatching period |
| CN115886160A (en) * | 2022-11-30 | 2023-04-04 | 华南农业大学 | A kind of feed for fruit fly larvae |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104095151A (en) * | 2013-04-02 | 2014-10-15 | 湖南师范大学 | Semi-artificial feed for Bactrocera minax larvae and preparation method thereof |
| CN103300257A (en) * | 2013-07-09 | 2013-09-18 | 海南出入境检验检疫局热带植物隔离检疫中心 | Artificial feed for bactrocera dorsalis larvas |
| CN104206695A (en) * | 2014-09-26 | 2014-12-17 | 张可池 | Mixed feed special for tachinid and preparation method thereof |
| CN104206695B (en) * | 2014-09-26 | 2016-08-24 | 张可池 | A kind of tachinid special compound feed and preparation method thereof |
| CN105166585A (en) * | 2015-10-28 | 2015-12-23 | 浙江省柑桔研究所 | Artificial feed for bactrocera tau adults and preparation method thereof |
| CN106036259A (en) * | 2016-05-30 | 2016-10-26 | 中国农业科学院植物保护研究所 | Artificial feed for larval delia antiqua (Meigen) as well as preparation method of artificial feed and raising method of larval delia antiqua (Meigen) |
| CN106173568A (en) * | 2016-07-22 | 2016-12-07 | 红河学院 | A kind of Fructus Capsici trypetid larva artificial feed and preparation method thereof |
| CN106306610A (en) * | 2016-08-23 | 2017-01-11 | 张驰 | Feed for larvae of apple flies and preparation method thereof |
| CN106689825A (en) * | 2016-12-05 | 2017-05-24 | 长沙秋点兵信息科技有限公司 | High-hatchability feed for bactrocera mediterranei larvae |
| CN106689826A (en) * | 2016-12-05 | 2017-05-24 | 长沙秋点兵信息科技有限公司 | Mediterranean fruit fly larva feed capable of shortening hatching period |
| CN115886160A (en) * | 2022-11-30 | 2023-04-04 | 华南农业大学 | A kind of feed for fruit fly larvae |
| CN115886160B (en) * | 2022-11-30 | 2024-05-28 | 华南农业大学 | A kind of feed for larvae of fruit fly of South Asia |
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Application publication date: 20130327 |