CN102934829A - Phosphate-rich health-care drink - Google Patents
Phosphate-rich health-care drink Download PDFInfo
- Publication number
- CN102934829A CN102934829A CN2012104838470A CN201210483847A CN102934829A CN 102934829 A CN102934829 A CN 102934829A CN 2012104838470 A CN2012104838470 A CN 2012104838470A CN 201210483847 A CN201210483847 A CN 201210483847A CN 102934829 A CN102934829 A CN 102934829A
- Authority
- CN
- China
- Prior art keywords
- mouse
- mda
- hepatic tissue
- drinking water
- control group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229910019142 PO4 Inorganic materials 0.000 title abstract description 4
- 239000010452 phosphate Substances 0.000 title abstract description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 title abstract description 4
- 235000013361 beverage Nutrition 0.000 claims abstract description 16
- 230000036541 health Effects 0.000 claims abstract description 15
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 235000013305 food Nutrition 0.000 claims description 10
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 2
- 239000003651 drinking water Substances 0.000 abstract description 35
- 108010017842 Telomerase Proteins 0.000 abstract description 34
- 235000020188 drinking water Nutrition 0.000 abstract description 34
- 230000000694 effects Effects 0.000 abstract description 23
- 230000032683 aging Effects 0.000 abstract description 19
- 230000035622 drinking Effects 0.000 abstract description 4
- 238000002360 preparation method Methods 0.000 abstract description 3
- 239000007864 aqueous solution Substances 0.000 abstract description 2
- 230000007774 longterm Effects 0.000 abstract description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 abstract 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 55
- 210000001519 tissue Anatomy 0.000 description 42
- 230000002440 hepatic effect Effects 0.000 description 41
- 230000004913 activation Effects 0.000 description 25
- 235000013399 edible fruits Nutrition 0.000 description 18
- 239000001963 growth medium Substances 0.000 description 15
- 238000001514 detection method Methods 0.000 description 11
- 230000003203 everyday effect Effects 0.000 description 9
- 229910052698 phosphorus Inorganic materials 0.000 description 8
- 241000588724 Escherichia coli Species 0.000 description 7
- 241000699670 Mus sp. Species 0.000 description 7
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 7
- 230000034994 death Effects 0.000 description 7
- 239000011574 phosphorus Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 241000255601 Drosophila melanogaster Species 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 240000008042 Zea mays Species 0.000 description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 6
- 210000004556 brain Anatomy 0.000 description 6
- 235000005822 corn Nutrition 0.000 description 6
- 235000013312 flour Nutrition 0.000 description 6
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 5
- 235000019733 Fish meal Nutrition 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 5
- 235000010469 Glycine max Nutrition 0.000 description 5
- 240000007594 Oryza sativa Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 239000002374 bone meal Substances 0.000 description 5
- 229940036811 bone meal Drugs 0.000 description 5
- 239000011575 calcium Substances 0.000 description 5
- 229910052791 calcium Inorganic materials 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000004467 fishmeal Substances 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 230000006698 induction Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 235000013619 trace mineral Nutrition 0.000 description 5
- 239000011573 trace mineral Substances 0.000 description 5
- 235000015099 wheat brans Nutrition 0.000 description 5
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000000392 somatic effect Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 238000010172 mouse model Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000001934 delay Effects 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000288673 Chiroptera Species 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001071917 Lithospermum Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 240000001307 Myosotis scorpioides Species 0.000 description 1
- 240000002547 Rosa roxburghii Species 0.000 description 1
- 235000000640 Rosa roxburghii Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 101000959126 Xenopus laevis Peptidyl-alpha-hydroxyglycine alpha-amidating lyase B Proteins 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 229910001410 inorganic ion Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002796 luminescence method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002367 phosphate rock Substances 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Images
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
本发明公开了一种富含磷酸盐的保健饮品。利用食品级磷酸二氢钾（KH2PO4），以一定浓度配制成饮用水，每升水溶液中含有80～400mg的食品级磷酸二氢钾（KH2PO4）。长期饮用，可以达到提高机体端粒酶活性的效果。本发明制备方法简单，成本低，对生物体无害。应用范围广，可以按照一定浓度添加到饮料中，坚持服用能起到延缓机体衰老的功效。
The invention discloses a health drink rich in phosphate. Food-grade potassium dihydrogen phosphate (KH 2 PO 4 ) is used to prepare drinking water at a certain concentration, and each liter of aqueous solution contains 80-400 mg of food-grade potassium dihydrogen phosphate (KH 2 PO 4 ). Long-term drinking can achieve the effect of increasing the activity of telomerase in the body. The preparation method of the invention is simple, low in cost and harmless to organisms. It has a wide range of applications and can be added to beverages at a certain concentration. Adherence to taking it can delay the aging of the body.
Description
Technical field
The present invention relates to a kind ofly can improve the phosphatic health beverages of being rich in of body telomerase activation, belong to the health beverages technical field that postpones biological decay.
Background technology
Along with social development and human life quality's raising, people more and more pay close attention to health, more and more expect life-time dilatation, and recent domestic bioscience worker utilizes fruit bat to study the impact of many factors on the life-span.The researchs such as Wu Yifan are found that Rosa roxburghii Normal juice has and are promoted the fruit rope to grow and the effect of delaying senility course.The researchs such as Xu Jinbo show that lithospermum euchromum Royle extract A E-I, AE-II all can extend average life span and the maximum life span of female male drosophila to fruit bat, and the survivorship curve of fruit bat is significantly moved to right.In yellow generation,, blue or green applied chemistry luminescence method research showed that the mulberry leaf extract has great scavenging action to the hydroxy radical in oxygen radical, when dose ratio traditional Chinese medical science voluminous dictionary regulation large is more than 3 times, do not reduce the average life span of fruit bat, and the maximum life span of fruit bat all is improved to some extent.Li Liande etc. study discovery, and the polysaccharide extracted from the Chinese caterpillar fungus submerged fermentation has the effect delayed senility to fruit bat, derive from the born of the same parents of AT01, the exocellular polysaccharide effect is best, and the effect of lengthening the life improves with the increase of purity.
The contained a great number of elements of organism comprises C, H, O, N, P, S, and wherein phosphorus is one of more element of body burden.Become phosphorus content in human body to be about 600-900g, 1% of about percentage of liveweight.It does not still form the important component of human body, and participates in some very important metabolic processes in vital movement.Therefore the abundant absorption of phosphorus has important effect to every vital movement of organism.
Zhongxiang City town at middle part, China Hubei Province have the title in long-lived county over thousand.Nowadays 77 centenarians are here living.Phosphorus ore town, Zhongxiang City is the highest De Yige of rock phosphate in powder grade town, the whole city.The dense distribution ground of these Elderly patients is phosphorus ore zone and the radiation circle thereof of Zhongxiang City exactly.The one of the main reasons of people from Zhongxiang City longevity may be because the P elements containing higher concentration in local domestic water causes.Therefore can infer, within the specific limits, the P elements of high concentration is taken in may have certain delaying senility function to human body.
Research shows, the absorption of P elements is mainly by flooding mechanism in animal body, with the form of inorganic ions, at enteron aisle, is absorbed.In addition, the Phosphorus From Wastewater element discharged at Phosphorus Mineral Area is mainly with KH
2PO
4Form exists, KH
2PO
4Be to allow the additive use in food, the service limits in beverage is 2g/Kg, to organism certain KH that feeds
2PO
4Can not produce injurious effects to body.Therefore, utilize phosphate KH
2PO
4The organism of feeding is expected to disclose the impact of absorption P elements on its various physical signs.
Summary of the invention
The object of the present invention is to provide and a kind ofly can improve the phosphatic health beverages of being rich in of body telomerase activation.
The composition that is rich in phosphatic health beverages of the present invention is: using water as solvent, the food grade monopotassium phosphate (KH that contains 80～400mg in every liter of solution
2PO
4).
The composition that is rich in phosphatic health beverages of the present invention is determined through great many of experiments.
In order to realize purpose of the present invention, the applicant has carried out following experimental study:
Mouse is divided into to 6 groups at random, 10 every group.Contrast CK group mouse is normally cultivated, and five processed group mouse all are established as aging model by back hypodermic injection D-galactolipin, are labeled as respectively T0～T4 group, in each processed group drinking water, add KH
2PO
4, make its final concentration be followed successively by 0mg/L, 3.2mg/L, 16mg/L, 80mg/L, 400mg/L.Every day the observed and recorded mouse amount of drinking water and weather, put to death mouse after 6 weeks, check the content of its brain, hepatic tissue telomerase activation and MDA (MDA).
By D-gal Aging model mice and control mice are fed and are contained different K H
2PO
4The drinking water of concentration, after 6 weeks, testing result shows: with control group, compare, in the brain of D-gal Aging model mice, hepatic tissue, the content of MDA obviously raises.Contain variable concentrations KH drinking
2PO
4Drinking water after, all can reduce in various degree the content of MDA in Mice Body.KH in drinking water
2PO
4When concentration is 80mg/L, reduce effect comparatively significantly (table 1).
Table 1 variable concentrations KH
2PO
4Impact on mouse aging brain hepatic tissue MDA (MDA) content
*: with the T0 group, compare, p<0.05, difference reaches the level of signifiance.
#: with control group, compare, p<0.05, difference reaches the level of signifiance
Detection to the activity of brain, hepatic tissue telomerase shows, the brain of D-gal Aging model mice, the activity of hepatic tissue telomerase obviously reduce, and add the KH of variable concentrations in the drinking water of mouse aging
2PO
4All can improve the activity of Telomerase, when adding KH
2PO
4When concentration is 80mg/L, the effect that telomerase activation improves is (table 2) the most obviously.
The impact of table 2 variable concentrations Phos on mouse aging brain hepatic tissue telomerase activation
*: with the T0 group, compare, p<0.05, difference reaches the level of signifiance.
#: with control group, compare, p<0.05, difference reaches the level of signifiance.
##:p<0.01, difference reaches the utmost point level of signifiance.
Detect the KH of knowing 80mg/L by mouse
2PO
4Solution can improve its telomerase activation and reduce MDA (MDA) content in its body, infers a certain amount of KH
2PO
4Solution affected to some extent on the organism life-span, and therefore utilizing Escherichia coli and fruit bat is experimental subjects, to adding respectively a certain amount of KH in its culture medium
2PO
4, detect the situation that affects on its growth.
To colibacillary experiment: inoculation DH5 α Escherichia coli are to LB culture medium (tryptone 10g, YE 5g, sodium chloride 5g, distilled water 1000ml, pH 7.0-7.2), after overnight incubation, inoculation 1mL with the bacterium liquid of concentration to containing respectively 0mg/L, 0.64mg/L, 3.2mg/L, 16mg/L, 80mg/L, 400mg/L, 2g/L, 5g/L, 10g/L, 25g/L KH
2PO
4The LB culture medium in, 37 ℃, the 160rpm shaking table is cultivated 4h.8000rpm, the centrifugal collection thalline of 5min, measure its weight.
Experimental result shows, adds the KH of variable concentrations in culture medium
2PO
4Colibacillary upgrowth situation is had a significant effect.The coli somatic net weight of take shows as index detects, and works as KH
2PO
4When concentration is 0.64～2000 mg/L, the coli somatic net weight is than not adding KH
2PO
4Contrast increase is in various degree all arranged, work as KH
2PO
4When concentration is 3.2mg/L, the coli somatic net weight reaches 85.35mg, than the thalline net weight 70.95mg of contrast, increases the most obvious.Therefore, add certain KH in culture medium
2PO
4Escherichia coli Growth is had to facilitation, add the KH of 3.2mg/L
2PO
4To Escherichia coli Growth facilitation maximum.KH in culture medium
2PO
4Concentration reaches 5000mg/L when above, and colibacillary thalline net weight shows gradually and reduces with respect to control group, therefore, and the KH of high concentration
2PO
4To Escherichia coli Growth inhibited (Fig. 1).
Experiment to fruit bat: take Ye Sheng Xing ?the abdomen fruit bat be experiment material, collect the virgin fly, be inoculated into and contain 0mg/L, 0.64mg/L, 3.2mg/L, 16mg/L, 80mg/L, 400mg/L KH
2PO
4Fruit bat culture medium (water 1L, corn flour 105g, sucrose 75g, agar 7.5g, propionic acid 6.25ml, dusty yeast 20g).Cultivated for 25 ℃, each is processed and inoculates altogether 40 of fruit bats.After inoculation, observe the fruit bat weather every day, and record the fruit bat death condition, draws the life span of drosophila melanogaster curve map.
Result of the test shows, to fruit bat a small amount of KH that feeds
2PO
4All the life-span of fruit bat can be extended in various degree.The KH added in culture medium
2PO
4When concentration is 16mg/L, the phenomenon that life span of drosophila melanogaster obtains prolongation is the most obvious.The KH added
2PO
4Concentration is during higher than 16mg/L, though life span of drosophila melanogaster extended, with KH in culture medium
2PO
4The degree that the increase life span of drosophila melanogaster of concentration extends presents downward trend gradually, therefore, adds the KH of 16mg/L in culture medium
2PO
4Concentration is to the existence of fruit bat the most favourable (Fig. 2).
In above experimental result, because Bacillus coli cells is the KH directly touched in culture medium
2PO
4Solution, absorption efficiency is high, so 3.2mg/L is its optimum concentration.In test to fruit bat, food and moisture are originated all from culture medium, so contain the KH of 16mg/L in culture medium
2PO
4Concentration is the most favourable to the existence of fruit bat.For the experimental result of mouse, only in drinking water, add KH in test
2PO
4, in its feeding feed stuff, do not add, so the KH that contains 80mg/L in its drinking water
2PO
4Telomerase activation raising and MDA (MDA) content to mouse reduce the most favourable.
Due to mouse, as mammiferous model organism, its life style is close to the mankind, therefore of the present inventionly is rich in the data result that phosphatic health beverages can mainly be used for reference mouse experiment, considers the KH of 80mg/L
2PO
4To improving the best results of mouse body telomerase activation, and the KH of 400mg/L
2PO
4Solution is to the raising effect of mouse telomerase activation and the KH of 80mg/L
2PO
4The solution effect remains basically stable.Therefore originally be rich in phosphatic health beverages and form the food stage KH that is defined as every premium on currency interpolation 80～400mg
2PO
4.
The best of phosphate health beverages of the present invention forms: the food grade monopotassium phosphate (KH that contains 80mg in every premium on currency solution
2PO
4).
The present invention is said, and to be rich in phosphatic health beverages preparation method simple, uses food stage KH
2PO
4Be mixed with the aqueous solution, directly utilize food stage KH
2PO
4Be configured to the drinking water of 80～400mg/L, wherein, water can be used: mineral water, pure water, running water all can.
Adhere to every day drinking, can play the health-care effect that improves the human body telomerase activation, delays senility.
According to the KH that adds 2000～5000 mg/L in Escherichia coli culture medium
2PO
4May be to the cell calculating that has a negative impact, if the KH added in mineral water
2PO
4Concentration is 400mg/L, human body take for each person every day this drink on be limited to 5 liters.
The beneficial effect that the present invention obtains is: this health beverages preparation method is simple, and long-term drinking can significantly improve the activity of body tissue telomerase, reduce the content of MDA (MDA) in body, the antagonism radical damage, thus play the positive role delayed senility, harmless to body.Can also add in beverage according to finite concentration, adhere to taking and can play the effect that delays body aging.
The accompanying drawing explanation
The KH that Fig. 1 is variable concentrations
2PO
4Impact on Escherichia coli Growth.Work as KH
2PO
4When concentration is 3.2mg/L, the coli somatic net weight reaches 85.35mg, than the thalline net weight 70.95mg of contrast, increases the most obvious.
Fig. 2 is variable concentrations KH
2PO
4Interpolation life span of drosophila melanogaster is affected, the KH added in culture medium
2PO
4When concentration is 16mg/L, the phenomenon that life span of drosophila melanogaster obtains prolongation is the most obvious.
The specific embodiment
The following examples are for the present invention is described, but protection scope of the present invention is not subject to the restriction of embodiment.
Embodiment 1
Get 20 of mouse, utilizing back hypodermic injection D-galactolipin method artificial induction is aging model.It is divided into to control group and processed group, the processed group drinking water consists of the food stage KH that contains 100mg in every premium on currency simultaneously
2PO
4, do not add KH in the control group drinking water
2PO
4Two groups of feed formulas are corn flour 27%, wheat bran 19%, rice 16%, soya-bean cake 16%, fish meal 13%, calcium powder 3%, bone meal 3%, dusty yeast 2.3%, salt 0.5%, B B-complex 0.1%, trace element 0.1%, do not do other any specially treateds.Every day the observed and recorded mouse amount of drinking water and weather, put to death mouse after 6 weeks, check the content of its hepatic tissue telomerase activation and MDA (MDA).
By the detection to processed group and control group mice, result shows: in the hepatic tissue of control group phase mouse, the content of MDA is 18.23 ± 0.68, with the KH of interpolation 100mg in drinking water
2PO
4In the hepatic tissue of processed group mouse, mda content 15.70 ± 0.12 has significant difference.It is 0.07 ± 0.006 that the hepatic tissue telomerase activation of control group phase mouse detects, and the hepatic tissue telomerase activation of processed group mouse is 0.10 ± 0.002 to have significant difference between the two.
Above-mentioned detection shows, adds the KH of 100mg in the drinking water of mouse aging in every premium on currency
2PO
4Can significantly improve the activity of its hepatic tissue Telomerase, reduce the mda content in its hepatic tissue.
Embodiment 2
Get 20 of mouse, utilizing back hypodermic injection D-galactolipin method artificial induction is aging model.It is divided into to control group and processed group simultaneously, adds the KH of 200mg/L in the processed group drinking water
2PO
4, do not add KH in the control group drinking water
2PO
4Two groups of feed formulas are corn flour 27%, wheat bran 19%, rice 16%, soya-bean cake 16%, fish meal 13%, calcium powder 3%, bone meal 3%, dusty yeast 2.3%, salt 0.5%, B B-complex 0.1%, trace element 0.1%, do not do other any specially treateds.Every day the observed and recorded mouse amount of drinking water and weather, put to death mouse after 6 weeks, check the content of its hepatic tissue telomerase activation and MDA (MDA).
By the detection to processed group and control group mice, result shows: in the hepatic tissue of control group phase mouse, the content of MDA is 19.26 ± 0.37, with the KH of interpolation 200mg/L in drinking water
2PO
4In the hepatic tissue of processed group mouse, mda content 16.85 ± 0.17 has significant difference.It is 0.09 ± 0.002 that the hepatic tissue telomerase activation of control group phase mouse detects, and the hepatic tissue telomerase activation of processed group mouse is 0.12 ± 0.011 to have significant difference between the two.
Above-mentioned detection shows, adds the KH of 200mg/L in the drinking water of mouse aging
2PO
4Can significantly improve the activity of its hepatic tissue Telomerase equally, reduce the mda content in its hepatic tissue.
Embodiment 3
Get 20 of mouse, utilizing back hypodermic injection D-galactolipin method artificial induction is aging model.It is divided into to control group and processed group simultaneously, adds the KH of 300mg/L in the processed group drinking water
2PO
4, do not add KH in the control group drinking water
2PO
4Two groups of feed formulas are corn flour 27%, wheat bran 19%, rice 16%, soya-bean cake 16%, fish meal 13%, calcium powder 3%, bone meal 3%, dusty yeast 2.3%, salt 0.5%, B B-complex 0.1%, trace element 0.1%, do not do other any specially treateds.Every day the observed and recorded mouse amount of drinking water and weather, put to death mouse after 6 weeks, check the content of its hepatic tissue telomerase activation and MDA (MDA).
By the detection to processed group and control group mice, result shows: in the hepatic tissue of control group phase mouse, the content of MDA is 18.21 ± 0.87, with the KH of interpolation 300mg/L in drinking water
2PO
4In the hepatic tissue of processed group mouse, mda content 16.12 ± 0.52 has significant difference.It is 0.07 ± 0.011 that the hepatic tissue telomerase activation of control group phase mouse detects, and the hepatic tissue telomerase activation of processed group mouse is 0.10 ± 0.005 to have significant difference between the two.
Above-mentioned detection shows, adds the KH of 300mg/L in the drinking water of mouse aging
2PO
4Equally also can significantly improve the activity of its hepatic tissue Telomerase, reduce the mda content in its hepatic tissue.
Embodiment 4
Get 20 of mouse, utilizing back hypodermic injection D-galactolipin method artificial induction is aging model.It is divided into to control group and processed group simultaneously, adds the KH of 400mg/L in the processed group drinking water
2PO
4, do not add KH in the control group drinking water
2PO
4Two groups of feed formulas are corn flour 27%, wheat bran 19%, rice 16%, soya-bean cake 16%, fish meal 13%, calcium powder 3%, bone meal 3%, dusty yeast 2.3%, salt 0.5%, B B-complex 0.1%, trace element 0.1%, do not do other any specially treateds.Every day the observed and recorded mouse amount of drinking water and weather, put to death mouse after 6 weeks, check the content of its hepatic tissue telomerase activation and MDA (MDA).
By the detection to processed group and control group mice, result shows: in the hepatic tissue of control group phase mouse, the content of MDA is 17.62 ± 0.59, with the KH of interpolation 400mg/L in drinking water
2PO
4In the hepatic tissue of processed group mouse, mda content 15.75 ± 0.64 has significant difference.It is 0.08 ± 0.007 that the hepatic tissue telomerase activation of control group phase mouse detects, and the hepatic tissue telomerase activation of processed group mouse is 0.10 ± 0.015 to have significant difference between the two.
Above-mentioned detection shows, adds the KH of 400mg/L in the drinking water of mouse aging
2PO
4Also can significantly improve the activity of its hepatic tissue Telomerase, reduce the mda content in its hepatic tissue.
Embodiment 5
Get 20 of mouse, utilizing back hypodermic injection D-galactolipin method artificial induction is aging model.It is divided into to control group and processed group simultaneously, adds the KH of 80mg/L in the processed group drinking water
2PO
4, do not add KH in the control group drinking water
2PO
4Two groups of feed formulas are corn flour 27%, wheat bran 19%, rice 16%, soya-bean cake 16%, fish meal 13%, calcium powder 3%, bone meal 3%, dusty yeast 2.3%, salt 0.5%, B B-complex 0.1%, trace element 0.1%, do not do other any specially treateds.Every day the observed and recorded mouse amount of drinking water and weather, put to death mouse after 6 weeks, check the content of its hepatic tissue telomerase activation and MDA (MDA).
By the detection to processed group and control group mice, result shows: in the hepatic tissue of control group phase mouse, the content of MDA is 17.49 ± 0.47, with the KH of interpolation 80mg/L in drinking water
2PO
4In the hepatic tissue of processed group mouse, mda content 14.63 ± 0.37 has utmost point significant difference.It is 0.06 ± 0.001 that the hepatic tissue telomerase activation of control group phase mouse detects, and the hepatic tissue telomerase activation of processed group mouse is 0.10 ± 0.002 to have utmost point significant difference between the two.
Above-mentioned detection shows, adds the KH of 80mg/L in the drinking water of mouse aging
2PO
4Can the utmost point significantly improve the activity of its hepatic tissue Telomerase, reduce the mda content in its hepatic tissue.
Claims (2)
1. one kind is rich in phosphatic health beverages, it is characterized in that it is composed as follows: using water as solvent, in every premium on currency solution, contain food grade monopotassium phosphate (KH
2PO
4) 80～400mg.
2. according to claim 1ly be rich in phosphatic health beverages, it is characterized in that: in every premium on currency solution, contain food grade monopotassium phosphate (KH
2PO
4) 80mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104838470A CN102934829A (en) | 2012-11-26 | 2012-11-26 | Phosphate-rich health-care drink |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104838470A CN102934829A (en) | 2012-11-26 | 2012-11-26 | Phosphate-rich health-care drink |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102934829A true CN102934829A (en) | 2013-02-20 |
Family
ID=47693788
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012104838470A Pending CN102934829A (en) | 2012-11-26 | 2012-11-26 | Phosphate-rich health-care drink |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102934829A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104509923A (en) * | 2013-12-26 | 2015-04-15 | 容宁 | Weakly alkaline drinking water |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0040654B1 (en) * | 1980-05-20 | 1984-04-18 | Vitapharm Pharmaceutical Pty Ltd. | Electrolyte drink concentrate |
| CN1137224A (en) * | 1994-08-26 | 1996-12-04 | 日本烟草产业株式会社 | PH regulator and drink prepared by using the same |
| JP2002369671A (en) * | 2001-06-15 | 2002-12-24 | Tosago:Kk | Mineral water obtained by blending concentrate of ocean deep water with head water |
| JP3761807B2 (en) * | 2001-10-15 | 2006-03-29 | 森永乳業株式会社 | Beverage with viscosity |
| CN102014674A (en) * | 2008-01-16 | 2011-04-13 | 热带产品公司 | Potassium fortification in beverages and methods thereof |
-
2012
- 2012-11-26 CN CN2012104838470A patent/CN102934829A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0040654B1 (en) * | 1980-05-20 | 1984-04-18 | Vitapharm Pharmaceutical Pty Ltd. | Electrolyte drink concentrate |
| CN1137224A (en) * | 1994-08-26 | 1996-12-04 | 日本烟草产业株式会社 | PH regulator and drink prepared by using the same |
| JP2002369671A (en) * | 2001-06-15 | 2002-12-24 | Tosago:Kk | Mineral water obtained by blending concentrate of ocean deep water with head water |
| JP3761807B2 (en) * | 2001-10-15 | 2006-03-29 | 森永乳業株式会社 | Beverage with viscosity |
| CN102014674A (en) * | 2008-01-16 | 2011-04-13 | 热带产品公司 | Potassium fortification in beverages and methods thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104509923A (en) * | 2013-12-26 | 2015-04-15 | 容宁 | Weakly alkaline drinking water |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104498382B (en) | A kind of microorganism formulation improved applied to culture environment of aquatic products and preparation method thereof | |
| CN102671009B (en) | Fermented plant extract and powder thereof with and combinations thereof the manufacture method of thing | |
| CN102293340B (en) | Compound premix able to improve immune function for pigs | |
| CN103421715B (en) | Lactobacillus rhamnosus and application thereof | |
| CN102450157A (en) | Solid fermentation method for cordyceps militaris | |
| CN105707453A (en) | Preparing method of feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients | |
| CN104450583A (en) | Preparation method of petroleum-degrading acinetobacter junii bacillus | |
| CN106922865A (en) | A kind of selenium-enriched dark tea and its processing method | |
| CN107006677A (en) | A feed rich in probiotics and active peptides and its application | |
| CN102907585B (en) | A kind of middle pig probiotic vitamin premix and batch | |
| CN104757560A (en) | Glutathione yeast enzymolysis solution composition and preparation method thereof | |
| CN110663595A (en) | A kind of culture method of tilapia | |
| CN104757275B (en) | Bacillus subtilis liquid fermentation radix glycyrrhizae probiotics, preparation method and its application as additive for farm animal feed | |
| CN102934829A (en) | Phosphate-rich health-care drink | |
| CN102676396B (en) | Paecilomyces tenuipes strain and application thereof | |
| CN104856063A (en) | Health calcium | |
| CN110663818A (en) | Fermented dandelion feed additive and preparation method and application thereof | |
| CN104322934B (en) | Boar feed additive and preparation method thereof | |
| CN105087410A (en) | Novel aquaculture probiotics, and production method and use thereof | |
| CN108851199B (en) | Preparation method of a compound humectant of enzymolysis glucomannan and medicinal fungus fermentation product | |
| CN102696879A (en) | Preparation parameter optimization method for astragalus polysaccharides and bacillus subtilis synbiotic compound, preparation process of astragalus polysaccharides and bacillus subtilis synbiotic compound and application of astragalus polysaccharides and bacillus subtilis synbiotic compound | |
| CN117384310A (en) | Preparation method and application of fucoidan | |
| CN105586267A (en) | Ganoderma lucidum mutant strain for production of Ganoderma lucidum mycelia | |
| CN102690147B (en) | Liquid nutrient medium for Irpex lacteus fermentation and preparation method thereof | |
| CN104892190B (en) | A kind of Se-rich lucid ganoderma culture medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130220 |