[go: up one dir, main page]

CN102815974A - Preparation method of composite microbe flush fertilizer - Google Patents

Preparation method of composite microbe flush fertilizer Download PDF

Info

Publication number
CN102815974A
CN102815974A CN2011101500720A CN201110150072A CN102815974A CN 102815974 A CN102815974 A CN 102815974A CN 2011101500720 A CN2011101500720 A CN 2011101500720A CN 201110150072 A CN201110150072 A CN 201110150072A CN 102815974 A CN102815974 A CN 102815974A
Authority
CN
China
Prior art keywords
preparation
towards
complex microorganism
fertilization
bacterial classification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2011101500720A
Other languages
Chinese (zh)
Inventor
高淑英
靳庆生
郭好礼
梁绍芬
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHANXI CHANGXIN BIOLOGICAL AGRICULTURAL TECHNOLOGY Co Ltd
Original Assignee
SHANXI CHANGXIN BIOLOGICAL AGRICULTURAL TECHNOLOGY Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHANXI CHANGXIN BIOLOGICAL AGRICULTURAL TECHNOLOGY Co Ltd filed Critical SHANXI CHANGXIN BIOLOGICAL AGRICULTURAL TECHNOLOGY Co Ltd
Priority to CN2011101500720A priority Critical patent/CN102815974A/en
Publication of CN102815974A publication Critical patent/CN102815974A/en
Pending legal-status Critical Current

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a preparation method of a composite microbe flush fertilizer. The method is characterized in that a plurality of microbes with different functionalities are cultured together in a same fermentation apparatus. According to the invention, with life activities between the plurality of microbes, and produced substances or a formed environment, the microbes are interdependent and are mutually benefited. At a same time, a preparation containing a broth of a plurality of microbes is obtained. With a concentration process, and with active substance added, a high-efficiency flush fertilizer product is prepared. The flush fertilizer product is a highly concentrated active product with a plurality of functional microbes. With the method, production efficiency is improved, production cost is greatly saved, and an application effect is good. The flush fertilizer is suitable for various agricultural products.

Description

Complex microorganism is towards the system of fertilization Preparation Method
Technical field
The present invention is specifically related to a kind of complex microorganism towards the system of fertilization Preparation Method, belongs to the field that bio-feritlizer is made, and is to adopt multiple different functionalities mikrobe, in same fermentation container, and co-cultivation.Obtain a kind of composite microbial bacteria liquid,, add active substance,, finally obtain a kind of novel complex microorganism and dash the fertilising product with organic carrier absorption through concentrating.
Background technology
Dash fertilising and be and a kind ofly widely use, receive one of fertilizer variety that peasant household welcomes deeply.Complex microorganism preparations, the product that is considered to have the mikrobe complex function.Adopt compound culture technique of mikrobe and concentration technology, obtain high-concentration bacterial liquid.Add the active substance of special efficacy,, process and dash fertilising with organic carrier absorption, can be more can stabilized product quality, strengthen application effect.Know with regard to the contriver, do not find that also making successful complex microorganism dashes fertilising, increases the report of a new kind for bio-feritlizer.
Summary of the invention
The object of the invention is exactly for bio-feritlizer increases a new kind, provides a kind of complex microorganism towards the system of fertilization Preparation Method.
Main technical schemes is: adopt multiple different functionalities mikrobe, in same fermentation container, compound cultivation through concentration technology, obtains high-concentration bacterial liquid.Through adding the active substance of special efficacy,, process and dash fertilising with organic carrier absorption.
Complex microorganism comprises function culture of strains, selection and combination towards the system of fertilization Preparation Method, the control of culture medium prescription and preparation, culture condition and design.It is characterized in that taking the speed of growth respectively and the cycle is different, nutrition complement, the multiple mikrobe that can reside in same environment altogether each other according to the physilogical characteristics of bacterial classification, through cultivating altogether, concentration technology obtains high-concentration compound microorganism bacterium liquid.Add the active substance of special efficacy,, process and dash fertilising with organic carrier absorption, can be more can stabilized product quality, strengthen application effect.
Described bacterial classification comprises multiple mikrobes such as bacillus, genus lactubacillus, Saccharomycodes, acetic acid Pseudomonas, Azotobacter, rhizobium.Has the function of producing various active enzyme, multiple organic acid, VITAMINs and fixed nitrogen, phosphorus decomposing, potassium decomposing, pest-resistant, disease-resistant, stimulating growth.Be applied in agricultural, can improve crop alimentary effectively, improve resistance, improve quality, increase output.
Describedly cultivating altogether, is to adopt the general deep liquid culture tank of fermentation industry, selects the substratum of suitable growth according to the needs of each bacterial classification, and aeration condition is cultivated, and thalline reaches maximum quantity, and maturation is put jar.
Described cultivation altogether also can adopt deep liquid to leave standstill cultivation as required, requires to select appropriate media according to each bacterial classification, and mainly be under anaerobic to make its fermentation, the viable bacteria and the tunning of acquisition some amount.
The slant medium that said process for preparing strain thereof is used has following component and preferable weight proportion: peptone 8~12g Carnis Bovis seu Bubali cream 4~6g glucose 1~3g agar 12~20g zero(ppm) water 1L.
The substratum that described bacterial classification liquid culture is used has following component and preferable weight proportion:
Soybean hydrolyzate 10~30g peptone 4~10g sucrose 16~24g KH 2PO 43~8g MgSO 41~6g water 1L.
Described bacterial classification deep liquid leaves standstill the substratum of cultivating usefulness, has following component and preferable weight proportion:
Sucrose 10~30g peptone 4~10g yeast extract paste 5-10g KH 2PO 45~10gMgSO 41~6g water 1L.
Described active substance has the activating soil nutritive substance, regulates the material of plant metabolism or stimulation plant growth.
Described organic carrier is selected type organic matter such as vegetable mould, careless carbon, weathered coal for use.
Complex microorganism of the present invention towards system of fertilization Preparation Method positively effect is: in same incubator, cultivate altogether and special producing technology obtains a kind of highly enriched biologically active prod of multiple functional microorganism that has through many bacterium.Improve production efficiency, practiced thrift production cost greatly.The advantage that result of use is good.
Be described further below in conjunction with instance, but not as to qualification of the present invention.
Embodiment
Instance 1.
Slant strains: bacillus megaterium 1., vinelandii are the test tube slant 2., in 25-32 ℃ of constant temperature culture, 24-72h.
Bacteria suspension: with 1. 2. test tube slant of bacterial classification, pour sterilized water respectively into, scrape and get lawn, the preparation bacteria suspension.
Bacterial classification mixes: with 1. 2. bacteria suspension of bacterial classification, proportional mixing.
Liquid nutrient medium: the substratum that the bacterial classification liquid culture is used has following component and weight proportion: soybean hydrolyzate 10~30g peptone 4~10g sucrose 16~24g KH 2PO 43~8g MgSO 41~6g water 1L.
Sterilization: 0.12-0.15Mpa, 121-125 ℃, steam sterilizing 30 minutes.
Inoculation: bacteria suspension is inserted in the substratum of sterilization.
Cultivate: 25-32 ℃, tank pressure 0.04-0.05Mpa cultivates 36-72h.
Concentrate: vacuum decompression concentrates.Add active substance.
Absorption: the vegetable mould sterilization mixes with bacterium liquid.
Instance 2.
Slant strains: milk-acid bacteria 3., sporeformer 4. the test tube slant in 25-38 ℃ of constant temperature culture, 24-72h.
Bacteria suspension: with 3. 4. test tube slant of bacterial classification, pour sterilized water respectively into, scrape and get lawn, the preparation bacteria suspension.
Bacterial classification mixes: with 3. 4. bacteria suspension of bacterial classification, proportional mixing.
Liquid nutrient medium: sucrose 10~30g peptone 4~10g yeast extract paste 5-10gKH 2PO 45~10g MgSO 41~6g water 1L
Sterilization: 0.12-0.15Mpa, 121-125 ℃, steam sterilizing 30 minutes.
Inoculation: bacteria suspension is inserted in the substratum of sterilization.
Cultivate: 25-32 ℃, cultivate 2-7d.
Concentrate: vacuum decompression concentrates.Add active substance.
Absorption: the vegetable mould sterilization mixes with bacterium liquid.
Instance 3.
Mix: instance 1. concentrates bacterium liquid with instance 2. to be mixed.
Absorption: the vegetable mould sterilization mixes with bacterium liquid.
Implementation result of the present invention: impose the negative impact that chemical fertilizer brings from radical change, prevent that fertilizer damage, poisoning and excessive from imposing the environmental pollution that chemical fertilizer causes.
The functional characteristics of this product:
1, highly enriched, full speed absorbs fast.Be to use the high reactivity ecoalimental body that modern biological skill special process is processed.Have biological metabolism can with multielement nutrient science compound, good synergistic function, plant recovery of nutrient is high.
2, activating soil, organic, the culture fertility of increase.There is improving effect in Baijiang soil, sandy soil, saltings, can regulates the soil water, fertilizer, gas, heat, chemical fertilizer, pesticide residue in the soil are decomposed in absorption, promote soil aggregate to form.
3, root of hair is fast, short living, short long short precocious.Can regulate the farm crop physiological process, enhancement of plant immunizing power has drought resisting, cold-resistant, resistant to lodging, anti-continuous cropping, anti-dead, preventing disease and pest, and is remarkable towards executing effect in good time especially in continuous cropping continuous cropping and saline and alkaline plot, obviously improves crop yield and quality comprehensively.
4, economy conveniently saves labor, suitable various farm crop and edaphic condition.
Application process
1, using sufficient on the basis of base fertilizer, every mu of usefulness is dashed 1 bag (5 kilograms) of fertilising.
2, in crops seedling stage, growth most productive period, reproduction productive phase, dash in good time and execute.Like every kind of farm crop whole growth phase, adhere to dashing and execute 2-3 time, raising the output 15-30%.
3, every bag stirs with the 30-50 kg of water earlier, and stream slowly pours downstream.

Claims (7)

1. complex microorganism is towards the system of fertilization Preparation Method; Comprise selection, combination and the cultivation of function bacterial classification; The control of culture medium prescription and preparation, culture condition and design; It is characterized in that taking the speed of growth respectively and the cycle is different, nutrition complement, two kinds of mikrobes can residing in same environment altogether each other,, obtain high-concentration compound microorganism bacterium liquid through cultivating altogether and concentration technology according to the physilogical characteristics of bacterial classification.Add active substance,, obtain a kind of complex microorganism and dash fertilising with organic carrier absorption.
2. complex microorganism according to claim 1 is characterized in that the selection of function bacterial classification towards the system of fertilization Preparation Method, adopts to comprise multiple mikrobes such as bacillus, genus lactubacillus, Saccharomycodes, acetic acid Pseudomonas, Azotobacter, rhizobium.Has the function of producing various active enzyme, multiple organic acid, VITAMINs and fixed nitrogen, phosphorus decomposing, potassium decomposing, pest-resistant, disease-resistant, stimulating growth.Be applied in agricultural, can improve crop alimentary effectively, improve resistance, improve quality, increase output.Select aerobic training method, two bacterium are cultivated.The inoculative proportion that two bacterium are cultivated: 0.1-2: 1-10.
3. complex microorganism according to claim 2 is characterized in that the function culture of strains towards the system of fertilization Preparation Method, has the ratio of soybean hydrolyzate and each component in the substratum: soybean hydrolyzate 10~50g peptone 4~10g sucrose 16~24g KH 2PO 43~8g MgSO 41~6g water 1L.
4. complex microorganism according to claim 2 is towards the system of fertilization Preparation Method; It is characterized in that spawn culture adopts bacterial classification in vital movement; Can produce various active enzyme and secondary metabolite, like the mikrobe of multiple organic acid, small-molecular peptides, VITAMINs, oligosaccharide substance.The anaerobism training method of selecting deep layer to leave standstill, compound cultivation.Inoculum size proportioning: 1: 5-40.
5. complex microorganism according to claim 4 is towards the system of fertilization Preparation Method, it is characterized in that having following component and weight proportion in the anaerobic culture medium that deep layer leaves standstill: sucrose 10~30g peptone 4~10g yeast extract paste 5-10g KH 2PO 45~10g MgSO 41~6g water 1L.
6. complex microorganism according to claim 1 is towards the system of fertilization Preparation Method, it is characterized in that active substance selects material with activating soil nutrition for use, regulates plant metabolism or stimulate the material of plant growth.
7. complex microorganism according to claim 1 is characterized in that organic carrier towards the system of fertilization Preparation Method, selects type organic matter such as vegetable mould, careless carbon, weathered coal for use.
CN2011101500720A 2011-06-07 2011-06-07 Preparation method of composite microbe flush fertilizer Pending CN102815974A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2011101500720A CN102815974A (en) 2011-06-07 2011-06-07 Preparation method of composite microbe flush fertilizer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2011101500720A CN102815974A (en) 2011-06-07 2011-06-07 Preparation method of composite microbe flush fertilizer

Publications (1)

Publication Number Publication Date
CN102815974A true CN102815974A (en) 2012-12-12

Family

ID=47300478

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011101500720A Pending CN102815974A (en) 2011-06-07 2011-06-07 Preparation method of composite microbe flush fertilizer

Country Status (1)

Country Link
CN (1) CN102815974A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105505823A (en) * 2015-12-31 2016-04-20 北京沃土天地生物科技有限公司 Compound microbial agent, water-soluble fertilizer containing same and applications of compound microbial agent and water-soluble fertilizer

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1092397A (en) * 1993-03-18 1994-09-21 中国科学院沈阳应用生态研究所 Multi-component organic composite fertilizer and preparation method thereof
CN1304912A (en) * 2000-12-31 2001-07-25 李承海 Process for preparing microecological organic fertilizer
US20080190158A1 (en) * 2007-02-09 2008-08-14 Unlv Biofertilizer for treatment to improve growth of turf grass and method of developing the biofertilizer
CN101805223A (en) * 2010-03-16 2010-08-18 范鸿基 High-efficiency biological soluble fertilizer and strain preparation thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1092397A (en) * 1993-03-18 1994-09-21 中国科学院沈阳应用生态研究所 Multi-component organic composite fertilizer and preparation method thereof
CN1304912A (en) * 2000-12-31 2001-07-25 李承海 Process for preparing microecological organic fertilizer
US20080190158A1 (en) * 2007-02-09 2008-08-14 Unlv Biofertilizer for treatment to improve growth of turf grass and method of developing the biofertilizer
CN101805223A (en) * 2010-03-16 2010-08-18 范鸿基 High-efficiency biological soluble fertilizer and strain preparation thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105505823A (en) * 2015-12-31 2016-04-20 北京沃土天地生物科技有限公司 Compound microbial agent, water-soluble fertilizer containing same and applications of compound microbial agent and water-soluble fertilizer

Similar Documents

Publication Publication Date Title
AU2009292604B2 (en) Antagonistic bacteria for controlling the fusarium wilt of continuous cropping banana and their microbial organic fertilizer
CN101734956B (en) Method for preparing microorganism fermentation liquor from fish skins or fish scales and bacterial manure containing fermentation liquor
CN101239847B (en) Liquid composite microbial fertilizer and its preparation method
CN101717315B (en) Special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof
CN101974428A (en) Complex microbial preparation capable of resisting replant obstacle resistance and preparation method thereof
CN109320345A (en) A composite microbial inoculant for improving crop yield and quality and use thereof
CN103834565A (en) Micro-ecological flora of crop root system probiotics and application thereof
CN104818233B (en) One plant of bacillus containing Death Valley and its functional form vegetables nursery bio-matrix of preparation
CN103194410A (en) Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same
CN100569704C (en) A K3 strain capable of dissolving calcium phosphate in soil and its microbial organic fertilizer
CN103484396A (en) New strain of streptomyces thermocarboxydus and application thereof
CN105316255B (en) A kind of method of soil beneficial microbe mixed fermentation
CN106946626A (en) Prevent composite microbic bacterial fertilizer of root knot nematode disease and preparation method thereof
CN103320371A (en) Bacterium having growth-promoting effect in synergism with AM fungus and application of bacterium in vegetable growth promoting
CN106661539A (en) Homogeneous microorganism extract using useful and functional microorganisms and method for producing same
CN101659570A (en) Preparation method of biofertilizer
CN102391972B (en) A cherry rhizosphere-promoting Pseudomonas and its application
CN102815973A (en) Preparation method of biological selenium-rich fertilizer
CN108484267A (en) A kind of complex microorganism ecological organic fertilier and its preparation method and application
CN108330092A (en) One plant of husky good fortune bacillus and its microbial inoculum and application with growth-promoting function
CN104860732A (en) Microbial strain organic fertilizer for improving soil alkalinity
CN109468343A (en) A kind of biogas promoter by anaerobic fermentation of straw and its preparation method and application
CN108947679A (en) A kind of microbial organic fertilizer and preparation method thereof
CN104498413B (en) Functional vegetable seedling biological matrix containing bacillus subtilis G10 and preparation method thereof
CN104774788B (en) Lawn salt tolerant strengthens the preparation method and application of complex microbial community in garbage compost

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20121212