CN102703546B - Preparation method of corn resistant starch - Google Patents
Preparation method of corn resistant starch Download PDFInfo
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- CN102703546B CN102703546B CN201210192270.8A CN201210192270A CN102703546B CN 102703546 B CN102703546 B CN 102703546B CN 201210192270 A CN201210192270 A CN 201210192270A CN 102703546 B CN102703546 B CN 102703546B
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- 229920000294 Resistant starch Polymers 0.000 title claims abstract description 36
- 235000021254 resistant starch Nutrition 0.000 title claims abstract description 36
- 240000008042 Zea mays Species 0.000 title claims abstract description 24
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 24
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 24
- 235000005822 corn Nutrition 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 241000499912 Trichoderma reesei Species 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 10
- 108010028688 Isoamylase Proteins 0.000 claims abstract description 6
- 229920002261 Corn starch Polymers 0.000 claims abstract 2
- 239000008120 corn starch Substances 0.000 claims abstract 2
- 229920002472 Starch Polymers 0.000 claims description 20
- 235000019698 starch Nutrition 0.000 claims description 20
- 239000008107 starch Substances 0.000 claims description 20
- 238000005406 washing Methods 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000000725 suspension Substances 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 238000009835 boiling Methods 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 5
- 239000000872 buffer Substances 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- XHFLOLLMZOTPSM-UHFFFAOYSA-M sodium;hydrogen carbonate;hydrate Chemical compound [OH-].[Na+].OC(O)=O XHFLOLLMZOTPSM-UHFFFAOYSA-M 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 102000011759 adducin Human genes 0.000 claims description 4
- 108010076723 adducin Proteins 0.000 claims description 4
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 239000002953 phosphate buffered saline Substances 0.000 claims description 4
- 210000000813 small intestine Anatomy 0.000 abstract description 6
- 238000000855 fermentation Methods 0.000 abstract description 5
- 230000004151 fermentation Effects 0.000 abstract description 5
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 abstract description 4
- 210000002429 large intestine Anatomy 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 3
- 206010010774 Constipation Diseases 0.000 abstract description 2
- 208000014617 hemorrhoid Diseases 0.000 abstract description 2
- 150000004666 short chain fatty acids Chemical class 0.000 abstract description 2
- 235000013325 dietary fiber Nutrition 0.000 abstract 2
- 241000305071 Enterobacterales Species 0.000 abstract 1
- 208000028774 intestinal disease Diseases 0.000 abstract 1
- 235000021391 short chain fatty acids Nutrition 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 239000000835 fiber Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 238000005191 phase separation Methods 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 208000034598 Caecitis Diseases 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 208000004387 Typhlitis Diseases 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000031891 intestinal absorption Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 235000021404 traditional food Nutrition 0.000 description 1
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of corn resistant starch. By using corn starch, and adopting a method of combining fermentation with Trichodermareesei and enzymolysis with isoamylase, the corn resistant starch is prepared. The corn resistant starch is not absorbed by small intestine like dietary fiber, and can unchangeably enter large intestine. Compared with dietary fiber, the corn resistant starch is more easily fermented by microorganisms in the large intestine, and partial corn resistant starch is utilized by fermentation of enterobacteria to generate short-chain fatty acids such as butyric acid. The corn resistant starch has important significance on preventing enteric diseases such as constipation, typhitis and haemorrhoids.
Description
Technical field
The present invention relates to a kind of preparation method of functional food, be specifically related to a kind of preparation method of resistant starch of corn.
Background technology
For a long time, people think that the starch in meals can be absorbed by complete hydrolysis at people's small intestine, but research is found in recent ten years, has small part starch to be subject to the impact of certain factor or the course of processing, and its structure changes, and produces anti-digestion phenomenon in small intestine.British scholar has found have a small amount of starch to remain in food fibre for 1985, and this starch is also anti-digestive ferment hydrolysis, so found Resistant starch.Starch is divided into three types: (1) is amyloclastic fast, after starch pasting, can in small intestine, digest rapidly, is generally the fresh starch-containing food boiling; (2) amyloclastic at a slow speed, digestion slowly in small intestine, the native starch particles of many cereal just belongs to this type; (3) Resistant starch, the not starch of digested enzymic hydrolysis.Europe Resistant starch association is defined as Resistant starch in 1992 " starch not absorbed by healthy individuals small intestine and the general name of degraded product thereof ".
Resistant starch is the same not by intestinal absorption with food fibre, can intactly enter large intestine, compared with food fibre, is more easily fermented by the microorganism in large intestine, and part produces short chain fatty acid as butyric acid etc. for entero-bacte fermentation utilization.In addition, Resistant starch is significant for intestinal tract diseases such as preventing constipation, typhlitis, hemorrhoid.Resistant starch more has superiority than traditional food fibre aspect a lot, such as it does not have special smell, particle diameter is less, retention ability is lower and more can adapt to multiple food-processing treatment and processing environment, has very much the potentiality of utilization.Relevant Study on Preparation of Resistant Starch, abroad development is very fast nearly ten years, studies very active, domestic in the ground zero stage.
Summary of the invention
The invention provides a kind of method of preparing Resistant starch taking W-Gum as raw material.The method, in conjunction with microorganism fermentation and enzyme process preparation, taking W-Gum as raw material, is prepared Resistant starch effectively.
Object of the present invention is achieved by the following technical programs:
The preparation method that a kind of resistant starch of corn is provided, comprises the following steps:
(1) take a certain amount of W-Gum, be placed in container, add sodium bicarbonate water soaking, process for 115 DEG C and within 20-30 minute, carry out gelatinization;
(2) W-Gum after gelatinization is cooled to room temperature, distilled water wash three times;
(3) W-Gum after washing is prepared into substratum, culture medium prescription is: W-Gum 50-100g/L, and peptone 5-10g/L, sodium-chlor 10-20g/L, extractum carnis 5-10g/L, all the other are water, regulate pH value to 6.0;
(4) in above-mentioned substratum, access Trichodermareesei (Trichoderma reesei), inoculum size is that 5%, 38 DEG C of culture volume ratio is cultivated 40-50 hour;
(5) by above-mentioned culture centrifuging and taking throw out, adopt three phase separation, obtain the starch of time heavy phase, use the phosphoric acid buffer of pH6.5 to wash three times, be finally prepared into suspension;
(6) in above-mentioned suspension, add isoamylase with the amount of 10U/1g dry starch, in 45 DEG C of enzymolysis 24-48 hour;
(7) after enzymolysis, adopt the boiling water enzyme that goes out, utilize afterwards 95% washing with alcohol 3 times, abandoning supernatant;
(8) throw out is dried, pulverize, cross 60 mesh sieves, obtain Resistant starch.
The invention has the beneficial effects as follows:
The present invention adopts microbe fermentation method and enzyme process combined utilization to prepare resistant starch of corn, for the preparation of Resistant starch provides one approach easily, has good industrial application prospect.
Embodiment
Further describe the present invention below in conjunction with specific embodiment.
Embodiment 1:
The preparation of resistant starch of corn:
Get 80g W-Gum, be placed in the triangular flask of 1L, adding massfraction is 10% sodium bicarbonate water 300mL, leaves standstill 5 hours, and 115 DEG C of gelatinizations are processed 25 minutes;
The W-Gum of above-mentioned gelatinization processing is cooled to room temperature, utilizes distilled water centrifuge washing three times, each centrifugal condition is 10000rpm, 10min;
W-Gum after washing is become to fermention medium according to following formulated: W-Gum 70g/L, peptone 8g/L, sodium-chlor 16g/L, extractum carnis 7g/L, all the other are water, regulate pH value to 6.0;
Inoculate Trichodermareesei (Trichoderma reesei) above-mentioned containing in the fermention medium of W-Gum, 5%, 38 DEG C of shaking table that inoculation volume is culture volume is cultivated 45 hours, and rotating speed is 200rpm;
Cultivate completely, by above-mentioned culture centrifuging and taking throw out, adopt three phase separation, the operating parameters of triphase separator is: rotary drum rotating speed 2500r/min, and poor rotating speed 40r/min, obtains the starch of time heavy phase;
By the phosphate buffered saline buffer centrifuge washing of 100mM concentration pH6.5 three times for above-mentioned W-Gum, each centrifugal condition is 10000rpm, and 10min, finally makes suspension;
In above-mentioned suspension, add the ratio of 10U to add isoamylase with every gram of dry starch, in 45 DEG C of enzymolysis 36 hours;
After enzymolysis, 10 minutes enzymes that go out of boiling water bath, are cooled to room temperature, centrifugal afterwards, taking precipitate, and centrifugal condition is 12000rpm, 20min;
Add the ratio of 50mL with every gram of throw out, add 95% ethanol, centrifuge washing 3 times, abandoning supernatant, centrifugal condition is 12000rpm, 20min;
Finally, the throw out obtaining, 80 DEG C of oven dry, is pulverized, crossed 60 mesh sieves, obtain resistant starch of corn.
Embodiment 2:
The preparation of resistant starch of corn:
Get 100g W-Gum, be placed in the triangular flask of 1L, adding massfraction is 10% sodium bicarbonate water 300mL, leaves standstill 5 hours, and 115 DEG C of gelatinizations are processed 30 minutes;
The W-Gum of above-mentioned gelatinization processing is cooled to room temperature, utilizes distilled water centrifuge washing three times, each centrifugal condition is 10000rpm, 10min;
W-Gum after washing is become to fermention medium according to following formulated: W-Gum 80g/L, peptone 10g/L, sodium-chlor 20g/L, extractum carnis 10g/L, all the other are water, regulate pH value to 6.0;
Inoculate Trichodermareesei (Trichoderma reesei) above-mentioned containing in the fermention medium of W-Gum, 5%, 38 DEG C of shaking table that inoculation volume is culture volume is cultivated 50 hours, and rotating speed is 200rpm;
Cultivate completely, by above-mentioned culture centrifuging and taking throw out, adopt three phase separation, the operating parameters of triphase separator is: rotary drum rotating speed 2500r/min, and poor rotating speed 40r/min, obtains the starch of time heavy phase;
By the phosphate buffered saline buffer centrifuge washing of 100mM concentration pH6.5 three times for above-mentioned W-Gum, each centrifugal condition is 10000rpm, and 10min, finally makes suspension;
In above-mentioned suspension, add the ratio of 10U to add isoamylase with every gram of dry starch, in 45 DEG C of enzymolysis 48 hours;
After enzymolysis, 10 minutes enzymes that go out of boiling water bath, are cooled to room temperature, centrifugal afterwards, taking precipitate, and centrifugal condition is 12000rpm, 20min;
Add the ratio of 50mL with every gram of throw out, add 95% ethanol, centrifuge washing 3 times, abandoning supernatant, centrifugal condition is 12000rpm, 20min;
Finally, the throw out obtaining, 80 DEG C of oven dry, is pulverized, crossed 60 mesh sieves, obtain resistant starch of corn.
Embodiment 3:
The preparation of resistant starch of corn:
Get 70g W-Gum, be placed in the triangular flask of 1L, adding massfraction is 10% sodium bicarbonate water 300mL, leaves standstill 5 hours, and 115 DEG C of gelatinizations are processed 20 minutes;
The W-Gum of above-mentioned gelatinization processing is cooled to room temperature, utilizes distilled water centrifuge washing three times, each centrifugal condition is 10000rpm, 10min;
W-Gum after washing is become to fermention medium according to following formulated: W-Gum 60g/L, peptone 5g/L, sodium-chlor 10g/L, extractum carnis 5g/L, all the other are water, regulate pH value to 6.0;
Inoculate Trichodermareesei (Trichoderma reesei) above-mentioned containing in the fermention medium of W-Gum, 5%, 38 DEG C of shaking table that inoculation volume is culture volume is cultivated 40 hours, and rotating speed is 200rpm;
Cultivate completely, by above-mentioned culture centrifuging and taking throw out, adopt three phase separation, the operating parameters of triphase separator is: rotary drum rotating speed 2500r/min, and poor rotating speed 40r/min, obtains the starch of time heavy phase;
By the phosphate buffered saline buffer centrifuge washing of 100mM concentration pH6.5 three times for above-mentioned W-Gum, each centrifugal condition is 10000rpm, and 10min, finally makes suspension;
In above-mentioned suspension, add the ratio of 10U to add isoamylase with every gram of dry starch, in 45 DEG C of enzymolysis 24 hours;
After enzymolysis, 10 minutes enzymes that go out of boiling water bath, are cooled to room temperature, centrifugal afterwards, taking precipitate, and centrifugal condition is 12000rpm, 20min;
Add the ratio of 50mL with every gram of throw out, add 95% ethanol, centrifuge washing 3 times, abandoning supernatant, centrifugal condition is 12000rpm, 20min;
Finally, the throw out obtaining, 80 DEG C of oven dry, is pulverized, crossed 60 mesh sieves, obtain resistant starch of corn.
The detection of resistant starch of corn:
In the time that resistant starch of corn is detected, adopt the conventional technology in this area to carry out.
Resistant starch of corn described in described embodiment 1-3 is observed under 2000 times in scanning electronic microscope, and gained resistant starch of corn particle is shaft-like, oval shape or spherical; Mean polymerisation degree DP82.35, average molecular mass Mn that reduction end method mensuration obtains banana natural resistance starch are 15623.86.X-diffracting spectrum is analyzed its crystalline texture and is C type, and average crystallite degree is 30.16%; Average initial gelatinization point, peak temperature and the end gelatinization point that differential scanning calorimeter records is respectively 57.65 DEG C, 71.32 DEG C and 94.23 DEG C, and evenly heat enthalpy is 5.356J/g.
The present embodiment is implemented under taking technical solution of the present invention as prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to the above embodiments.
Claims (2)
1. a preparation method for resistant starch of corn, is characterized in that comprising the following steps:
(1) extracting corn starch, is placed in triangular flask, and adding massfraction is 10% sodium bicarbonate water, leaves standstill 5 hours, processes 20-30 minute for 115 DEG C afterwards;
(2) W-Gum step (1) being obtained is cooled to room temperature, utilizes distilled water centrifuge washing three times;
(3) W-Gum step (2) being obtained becomes fermention medium according to following formulated: W-Gum 60-80g/L, and peptone 5-10g/L, sodium-chlor 10-20g/L, extractum carnis 5-10g/L, all the other are water, regulate pH value to 6.0;
(4) in step (3) fermention medium, inoculate Trichodermareesei (Trichoderma reesei), 5%, 38 DEG C of shaking table that inoculation volume is culture volume is cultivated 40-50 hour, and rotating speed is 200rpm;
(5) culture centrifuging and taking throw out step (4) being obtained, adopts triphase separator to separate, and collects the W-Gum of time heavy phase;
(6) W-Gum concentration step (5) being obtained is 100mM, and the phosphate buffered saline buffer washing of pH6.5 three times, finally makes suspension;
(7) in the suspension obtaining in step (6), add the ratio of 10U to add isoamylase with every gram of dry starch, in 45 DEG C of enzymolysis 24-48 hour;
(8), after step (7) enzymolysis, 10 minutes enzymes that go out of boiling water bath, are cooled to room temperature, afterwards centrifuging and taking throw out;
(9) in the throw out obtaining in step (8), add the ratio of 50mL with every gram of throw out, add 95% ethanol, centrifuge washing 3 times, abandoning supernatant;
(10) throw out step (9) being obtained, 80 DEG C of oven dry, is pulverized, and crosses 60 mesh sieves, obtains resistant starch of corn.
2. the preparation method of resistant starch of corn according to claim 1, the centrifugal condition that it is characterized in that step (8) and (9) is 12000rpm, 20min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210192270.8A CN102703546B (en) | 2012-06-12 | 2012-06-12 | Preparation method of corn resistant starch |
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| CN201210192270.8A CN102703546B (en) | 2012-06-12 | 2012-06-12 | Preparation method of corn resistant starch |
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| CN102703546B true CN102703546B (en) | 2014-07-09 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101427741A (en) * | 2008-12-09 | 2009-05-13 | 吉林农业大学 | Method for preparing resistant starch of corn |
| DE602004023750D1 (en) * | 2003-10-24 | 2009-12-03 | Bayer Cropscience Ag | USE OF LINEAR POLY-ALPHA-1,4-GLUCANES AS A RESISTANT STRENGTH |
| CN101663402A (en) * | 2007-03-06 | 2010-03-03 | 泰特&莱尔组分美国公司 | Production of resistant starch products |
| CN101979638A (en) * | 2010-09-28 | 2011-02-23 | 南宁创新科技医药技术有限公司 | Method for preparing crosslinked porous cassavastarch |
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2012
- 2012-06-12 CN CN201210192270.8A patent/CN102703546B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE602004023750D1 (en) * | 2003-10-24 | 2009-12-03 | Bayer Cropscience Ag | USE OF LINEAR POLY-ALPHA-1,4-GLUCANES AS A RESISTANT STRENGTH |
| CN101663402A (en) * | 2007-03-06 | 2010-03-03 | 泰特&莱尔组分美国公司 | Production of resistant starch products |
| CN101427741A (en) * | 2008-12-09 | 2009-05-13 | 吉林农业大学 | Method for preparing resistant starch of corn |
| CN101979638A (en) * | 2010-09-28 | 2011-02-23 | 南宁创新科技医药技术有限公司 | Method for preparing crosslinked porous cassavastarch |
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