CN102487801B - Method for soilless culture of bulblets of tissue culture seedlings of oriental lily - Google Patents

Abstract

The invention discloses a method for cultivating seed bulbs of oriental lily tissue culture seedlings without soil, which belongs to the technical field of flower cultivation and breeding. The steps of the method of the present invention are as follows: the oriental lily tissue culture seedlings are treated at 3-5°C for 6-8 weeks before soilless cultivation, and after two growing seasons of cultivation, the N-P-K-Mg ratio in the first year is: after planting, the 4-8 The 11th week was 80-30-40-24mg/L, the 12th-24th week was 200-60-200-48mg/L, and the 25th-30th week was 0-60-400-0mg/L. The ratio of N-P-K-Mg in the second year is: 80-30-80-24mg/L in the 4th-11th week after planting, 100-40-200-36mg/L in the 12th-24th week, and 25-30 weeks after planting. 100-30-300-0mg/L. During the growth period, the ratio of 1:1 to 1:1 is mixed with Junlimiil and green carbendazim, and the ratio of 1:1:1 to mix hymexazol, Zhongshengmycin and Penkang, and the two combinations are used alternately to prevent and control diseases. After the first round of cultivation, the average weight of the small balls increases by 8-11 times, and the diameter increases by 3-4 times. After the second round of cultivation, the average weight of the small balls increases by 7-8 times, and the diameter increases by 2-3 times. The invention has the advantages of simple operation, rapid growth and expansion of lily bulbs, short breeding time and accelerated commercialization of bulbs, and can be widely used in the breeding and cultivation of oriental lily flowers.

Description

A kind of method for soilless cultivation of oriental lily tissue culture seedling ball

Technical field:

The invention relates to a method for soilless cultivation of oriental lily tissue cultured seedlings, belonging to the technical field of flower cultivation and breeding.

Background technique:

Oriental lily is the most popular high-grade flower in the market, and its breeding work is very important, but it is found in practice that the growth cycle of oriental lily bulbs is relatively long. In scientific research and production practice, oriental lily needs to be cultivated for more than 3 years to become a commercial bulb through tissue culture, scale cutting propagation and seed propagation. This is a major obstacle to the commercial production of bulbs in oriental lily breeding and cultivation.

Existing studies mainly focus on the effects of commercial lily cultivation, different fertilization formulas and different cultivation substrates on the quality of lily cut flowers. There are few studies on the soilless cultivation of lily bulbs and the optimization of nutrient solution formula.

Through document search, do not see the public report identical with the present invention.

Invention content:

The purpose of the present invention is to overcome the deficiencies of the prior art, and provide a method for cultivating oriental lily seedling bulbs without soil, so as to speed up the breeding of oriental lily bulbs and the commercialization of newly bred varieties.

The study found that Oriental lily tissue culture seedlings were treated at low temperature first, then transplanted into soilless culture medium, and fertilized with optimized nutrient solution. After two growing seasons of cultivation, the average weight of the small balls increased by 8-8% after the first year of cultivation. 11 times, increase the circumference by 3-4 times, the average weight gain of the seed balls in the second year after cultivation is 7-8 times, and increase the circumference by 2-3 times.

The method for soilless cultivation of oriental lily tissue culture seedling balls of the present invention, its concrete steps are as follows:

a. Oriental lily tissue culture seedlings were treated at 3-5°C for 6-8 weeks;

b. Put the peat moss: perlite = 1:1 special substrate for lily seedlings with a water content of 65% and 50 times of formalin disinfection, seal it for 3-5 days, dry it for 7 days, and put it into a cultivation box with a thickness of 15-20cm ;The day before planting the seedlings, water the substrate thoroughly, and set aside; wash the culture medium adhered to the tissue culture seedlings, remove clustered buds, withered yellow leaves, keep single plants, and plant them in the cultivation box at a depth of 3-5 cm;

c. No nutrient solution is applied in the slow seedling stage in the first 1-3 weeks after planting, but attention should be paid to replenishing water; the formula of nutrient solution in the 4th-11th week after planting is: N-P-K-Mg=80-30-40-24 mg/L, the nutrient solution formula for the 12th-24th week after planting is: N-P-K-Mg=200-60-200-48 mg/L, the nutrient solution formula for the 25th-30th week after planting is: N-P-K-Mg=0 -60-400-0 mg/L; add 1/10 times the general trace elements of flowers to the above nutrient solution; apply the nutrient solution once a week, 2L each time; dig lily bulbs 2-3 weeks after fertilization;

d. Store the excavated small bulbs of lily bulbs in a ventilated refrigerator at -1°C. Take out the bulbs in March of the next year and plant them in the cultivation box after routine disinfection. The method of substrate disinfection and fertilization is the same as the previous year; Zhou’s nutrient solution formula is: N-P-K-Mg=80-30-80-24 mg/L, the nutrient solution formula for the 12th-24th week after planting is: 100-40-200-36 mg/L, and the 25th week after planting -The nutrient solution formula for 30 weeks is: 100-30-300-0 mg/L; 1/10 times the general trace elements of flowers are added to the above nutrient solution;

e. During the growth period, the ratio of 1:1 to 1:1 should be mixed with Bacteria and Green Carbendazim, and the ratio of 1:1:1 should be used to mix Hymexazol, Zhongshengmycin, and Penkang. The two combinations should be used alternately. Apply once a month, mainly to water the substrate to prevent and control diseases.

Oriental lily tissue culture seedlings are cultured without soil in the above two growing seasons. After the first round of cultivation, the average weight of the small balls increases by 8-11 times, and the circumference increases by 3-4 times. After the second round of cultivation, the average weight of the small balls increases. 7-8 times, increase the circumference 2-3 times.

Compared with the conventional method, the invention has the advantages of simple operation, rapid growth and expansion of lily bulbs, shorter breeding time, and accelerated commercialization of bulbs, and can be widely used in the breeding and cultivation of oriental lilies.

Specific implementation method:

The method for soilless cultivation of oriental lily tissue culture seedling balls of the present invention, its concrete steps are as follows:

a. Oriental lily tissue culture seedlings were treated at 3°C or 5°C for 6 weeks or 7 weeks or 8 weeks;

b. Seal the peat moss: perlite = 1:1 special substrate for lily seedlings with a water content of 65% and 50 times of formalin disinfection, seal it for 3 days or 5 days, dry it for 7 days, and put it into a cultivation box with a thickness of 15 cm or 20cm; the day before planting the seedlings, water the substrate thoroughly and set aside; wash the medium adhered to the tissue culture seedlings, remove clustered buds and withered yellow leaves, keep a single plant, and plant it in a cultivation box at a depth of 3 cm or 5 cm;

c. No nutrient solution is applied in the slow seedling stage in the first 1-3 weeks after planting, but attention should be paid to replenishing water; the formula of nutrient solution in the 4th-11th week after planting is: N-P-K-Mg=80-30-40-24 mg/L, the nutrient solution formula for the 12th-24th week after planting is: N-P-K-Mg=200-60-200-48 mg/L, the nutrient solution formula for the 25th-30th week after planting is: N-P-K-Mg=0 -60-400-0 mg/L; add 1/10 times the general trace elements of flowers to the above nutrient solution; apply the nutrient solution once a week, 2L each time; dig lily bulbs 2-3 weeks after fertilization;

d. Store the excavated small bulbs of lily bulbs in a ventilated refrigerator at -1°C. Take out the bulbs in March of the next year and plant them in the cultivation box after routine disinfection. The method of substrate disinfection and fertilization is the same as the previous year; Zhou’s nutrient solution formula is: N-P-K-Mg=80-30-80-24 mg/L, the nutrient solution formula for the 12th-24th week after planting is: 100-40-200-36 mg/L, and the 25th week after planting -The nutrient solution formula for 30 weeks is: 100-30-300-0 mg/L; 1/10 times the general trace elements of flowers are added to the above nutrient solution;

e. During the growth period, the ratio of 1:1 to 1:1 should be mixed with Bacteria and Green Carbendazim, and the ratio of 1:1:1 should be used to mix Hymexazol, Zhongshengmycin, and Penkang. The two combinations should be used alternately. Apply once a month, mainly to water the substrate to prevent and control diseases.

Oriental lily tissue culture seedlings are cultured without soil in the above two growing seasons. After the first round of cultivation, the average weight of the small balls increases by 8-11 times, and the circumference increases by 3-4 times. After the second round of cultivation, the average weight of the small balls increases. 7-8 times, increase the circumference 2-3 times.

The inventor has used this method in the bulb breeding of oriental lily. Practice has proved that this method is not only easy to operate and low in cost, but also has a fast speed of breeding bulbs through tissue cultured seedlings and a short commercialization time, which is 1 year shorter than the conventional method as a whole.

Claims (1)

1. a method for soilless cultivation of lily oriental tissue culture seedling balls, is characterized in that the concrete steps of the method are as follows: a. Oriental lily tissue culture seedlings were treated at 3-5°C for 6-8 weeks; b. Put the peat moss: perlite = 1:1 special substrate for lily seedlings with a water content of 65% and 50 times of formalin disinfection, seal it for 3-5 days, dry it for 7 days, and put it into a cultivation box with a thickness of 15-20cm ;The day before planting the seedlings, water the substrate thoroughly, and set aside; wash the culture medium adhered to the tissue culture seedlings, remove clustered buds, withered yellow leaves, keep single plants, and plant them in the cultivation box at a depth of 3-5 cm; c. No nutrient solution is applied in the slow seedling stage in the first 1-3 weeks after planting, but attention should be paid to replenishing water; the formula of nutrient solution in the 4th-11th week after planting is: N-P-K-Mg=80-30-40-24 mg/L, the nutrient solution formula for the 12th-24th week after planting is: N-P-K-Mg=200-60-200-48 mg/L, the nutrient solution formula for the 25th-30th week after planting is: N-P-K-Mg=0 -60-400-0 mg/L; add 1/10 times the general trace elements of flowers to the above nutrient solution; apply the nutrient solution once a week, 2L each time; dig lily bulbs 2-3 weeks after fertilization; d. Store the excavated small bulbs of lily bulbs in a ventilated refrigerator at -1°C. Take out the bulbs in March of the next year and plant them in the cultivation box after routine disinfection. The method of substrate disinfection and fertilization is the same as the previous year; Zhou’s nutrient solution formula is: N-P-K-Mg=80-30-80-24 mg/L, the nutrient solution formula for the 12th-24th week after planting is: 100-40-200-36 mg/L, and the 25th week after planting -The nutrient solution formula for 30 weeks is: 100-30-300-0 mg/L; 1/10 times the general trace elements of flowers are added to the above nutrient solution; e. During the growth period, the ratio of 1:1 to 1:1 should be mixed with Bacteria and Green Carbendazim, and the ratio of 1:1:1 should be used to mix Hymexazol, Zhongshengmycin, and Penkang. The two combinations should be used alternately. Apply once a month, mainly to water the substrate to prevent and control diseases.