CN102475909A - Porous scaffold material for tissue engineering - Google Patents
Porous scaffold material for tissue engineering Download PDFInfo
- Publication number
- CN102475909A CN102475909A CN2010105534416A CN201010553441A CN102475909A CN 102475909 A CN102475909 A CN 102475909A CN 2010105534416 A CN2010105534416 A CN 2010105534416A CN 201010553441 A CN201010553441 A CN 201010553441A CN 102475909 A CN102475909 A CN 102475909A
- Authority
- CN
- China
- Prior art keywords
- high polymer
- gene recombination
- porous support
- spider
- support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000463 material Substances 0.000 title claims abstract description 34
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 39
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 18
- 229920000642 polymer Polymers 0.000 claims abstract description 14
- 230000006798 recombination Effects 0.000 claims description 16
- 238000005215 recombination Methods 0.000 claims description 16
- 241000239290 Araneae Species 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 10
- 229920000954 Polyglycolide Polymers 0.000 claims description 9
- 239000004633 polyglycolic acid Substances 0.000 claims description 9
- 229920001661 Chitosan Polymers 0.000 claims description 6
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 6
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 6
- 239000004626 polylactic acid Substances 0.000 claims description 5
- -1 acrylic ester Chemical class 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 229920002101 Chitin Polymers 0.000 claims description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 2
- 125000001931 aliphatic group Chemical group 0.000 claims description 2
- 150000008064 anhydrides Chemical class 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 229920001577 copolymer Polymers 0.000 claims description 2
- 150000002148 esters Chemical class 0.000 claims description 2
- JMANVNJQNLATNU-UHFFFAOYSA-N glycolonitrile Natural products N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 claims description 2
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 2
- 229920002627 poly(phosphazenes) Polymers 0.000 claims description 2
- 229920001610 polycaprolactone Polymers 0.000 claims description 2
- 239000004632 polycaprolactone Substances 0.000 claims description 2
- VPVXHAANQNHFSF-UHFFFAOYSA-N 1,4-dioxan-2-one Chemical compound O=C1COCCO1 VPVXHAANQNHFSF-UHFFFAOYSA-N 0.000 claims 1
- 229920002959 polymer blend Polymers 0.000 claims 1
- 229920001872 Spider silk Polymers 0.000 abstract description 12
- 238000002156 mixing Methods 0.000 abstract description 9
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000012545 processing Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000003519 biomedical and dental material Substances 0.000 abstract 1
- 230000007850 degeneration Effects 0.000 description 13
- 239000007788 liquid Substances 0.000 description 12
- 239000003361 porogen Substances 0.000 description 12
- 235000018102 proteins Nutrition 0.000 description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000012620 biological material Substances 0.000 description 7
- 239000012153 distilled water Substances 0.000 description 7
- 238000007654 immersion Methods 0.000 description 7
- 108010022355 Fibroins Proteins 0.000 description 6
- 102000008186 Collagen Human genes 0.000 description 5
- 108010035532 Collagen Proteins 0.000 description 5
- 238000004108 freeze drying Methods 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 4
- 239000012467 final product Substances 0.000 description 4
- 239000006260 foam Substances 0.000 description 4
- 235000019253 formic acid Nutrition 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 239000012460 protein solution Substances 0.000 description 3
- 125000001424 substituent group Chemical group 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 108010028203 spidroin 2 Proteins 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 1
- 102000016942 Elastin Human genes 0.000 description 1
- 108010014258 Elastin Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003398 denaturant Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229920002549 elastin Polymers 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 125000004836 hexamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000009465 prokaryotic expression Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Images
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention belongs to the field of biomedical material scientific engineering, and relates to a porous scaffold material for tissue engineering. The tissue engineering scaffold material is obtained by blending the gene recombinant spider silk protein and the biodegradable high polymer, wherein the ratio (mass ratio) of the gene recombinant spider silk protein to the biodegradable high polymer is as follows: the porous scaffold material obtained by the invention has good biocompatibility, degradability and superior mechanical properties, and not only has simple processing technology and low cost, but also is suitable for industrialized mass production.
Description
Technical field
The invention belongs to field of biomedical materials, relate to a kind of tissue engineering rack material specifically.
Background technology
The depletion of tissue and organ, damage are topmost clinical medicine problems; And present Therapeutic Method mainly is organ transplantation, surgical repair, artificial substituent; Though these methods can play a role; But all there is such or such deficiency in they, are the method that cost " cures the wound with wound " like organ transplantation to sacrifice health tissues; There is the problem of biocompatibility in existing artificial substituent.Up to the eighties in 20th century; American scholar has proposed the organizational project regenerative medicine; It is principle and a method of utilizing life sciences and engineering science, and research and development is used for substituting tissue or the part of organ or substituent (Langer R, the Vacanti J P.Tissue engineering.Science of repertoire; 1993,260:920) 0 group
The development of weaver's journey has improved the depletion of tissue engineering tissue and organ, the treatment level of damage, has improved patient's quality of life, reduces medical treatment cost effectively.
One of main problem in science of tissue engineering research is to supply cell to carry out the interaction of timbering material and the cell and the timbering material of vital movement, and core is to set up the three dimensions complex that is made up of cell and biomaterial.Ideal biomaterial plays a crucial role in organizational project, and becomes the main flow of Tissue Engineering Study.
Experienced first generation inert material in the research of biomaterial, the second filial generation has activity or has after the degraded character, has developed into having degradable and bioactive third generation biomaterial (L.L.Hench etal. Science, 2002,295:1014) o concurrently
At present; Natural biologic material mainly is collagen protein in the organizational project; The substitute of synthetic mainly is that polylactic acid (PLA), polyglycolic acid (PGA) and polylactic acid and polyglycolic acid copolymer (PLGA) o will get ideal natural scaffold materials certain difficulty is arranged; And have some problems, strong like the antigenicity of collagen protein, mechanical strength is not enough, easy degeneration of collagen protein etc. in processing procedure; Though the substitute of synthetic also has biodegradable characteristics; Material implanted like U.S. FDA approval; Be made into absorbable suture, clamping plate, screw and dressing etc., but these materials need improve also at aspects such as the control of biocompatibility, physicochemical property, degradation rate and slow-releasing.Therefore, but at present the emphasis point of research more be to seek synthetic, good biocompatibility, degradable timbering material.
As the third generation biological material exploitation of organizational project, spider silk fibroin has advantageous condition.The Aranea cortina has the permeability of the good transparency, biodegradability and water one air interface.Similar with elastin laminin with collagen protein, spider silk fibroin tool self-assembly character is regulated so that mechanical support to be provided through secondary structure; Compare with polyester, the pliability and the elasticity of silk make it stand weight and fatigue.The fibroin good biocompatibility plays same cell adhesion, expansion, differentiation and growth with collagen.Silk substrate also has the mechanical induction effect, through the hardness of adjustment silk substrate, provides the final mechanical property of control substrate to imitate the mechanical property of natural body tissue and support growth in the host tissue.Therefore it shows great application potential (Winkler S on medical application, surgical sutures, biomaterial lining form and support, cell growth supporting bracket and sustained release substrate; Kaplan DL.Molecular biology of spidersilk. Reviews in Molecular Biotechnology; 2000,74:85).
The Li Min of Fujian Normal University etc. under the support of Fujian Province's natural science fund major scientific and technological projects (2001F 006), Ministry of Education's key project (02072) and project of national nature science fund project (30370414) respectively at (Li Min, Zhang Wenxian, Huang Jiankun etc. in 2002; The structure of spider dragline silk GFP and the expression in escherichia coli, biological engineering journal, 2002; 331) and (Li Min in 2004 18 (3):; Huang Jiankun, Tu Guiyun etc., structure, the expression and purification of RGD-spider dragline silk GFP; Biomedical engineering's magazine; 2004,21 (6): 1006) successively disclose the 26S Proteasome Structure and Function characteristic of this research group according to spider silk, using gene engineering is the modern biotechnology of core; Utilize the prokaryotic expression system; In conjunction with shot gun method and physical map spectrometry the full gene of spider dragline silk albumen is cloned, checked order and expresses, made up the spider's thread protein gene of multiple special sequence, but set up the technology such as fermentation tank high density fermentation process conditions of production; Make the public to have established the research and development basis for widely applying spider silk fibroin as the organizational project new material with simple and easy to do, low-cost and effective isolation and purification method scale preparation recombinant spider silk protein.
Summary of the invention
In order to solve the deficiency of existing tissue engineering material in biocompatibility, biological degradability and mechanical performance; The object of the invention be exactly according to the unique mechanical property of spider silk and biocompatibility with and essence be proteinic construction features, but the recombinant spider silk proteins and the biodegradable high polymer of disclosed scale preparation prepare good biocompatibility, degradable tissue engineering bracket material for primary raw material in utilization.This timbering material can not only be degraded to human body can absorb aminoacid, has good biocompatibility and mechanical performance preferably, and can produce in batches.
For realizing that the technical scheme that the object of the invention adopted is: with gene recombination spider silk fibroin and biodegradable high polymer is that primary raw material prepares good biocompatibility, degradable timbering material.Wherein the shared mass percent of gene recombination spider silk fibroin is 55-95%, and the shared mass percent of biodegradable high polymer is 45-50%.
Biodegradable high polymer is meant polylactic acid (PLA), polyglycolic acid (PGA), gathers breast among the present invention
Acid and polyglycolic acid copolymer (PLGA), paracyanogen for acrylic ester, polycaprolactone, gather the dioxa hexamethylene
Ketone and copolymer thereof, gather anhydride, poe, poly phosphazene, poly butyric, other linear aliphatic adoptions
Ester, polyamino acid, chitin, chitosan, cellulose, polyvinyl alcohol, in the polyoxyethylene a kind of or they
Combination.
The present invention can prepare porous support materials with the pore method, concrete technology:
1. the preparation support prepares liquid: get the gene recombination spider's thread protein powder and be dissolved in 80-98% formic acid and obtain egg
White solution adds high polymer again, and mixing promptly obtains containing the support preparation of gene recombination spider's thread protein and high polymer
Liquid.Wherein the shared mass percent of gene recombination spider's thread protein is 55-95%, and biodegradable height gathers
The shared mass percent of thing is 45-50%.
2. interpolation porogen: add porogen such as sodium chloride, mixing.
3. reverse mould, heating: the support that will be added with porogen prepares liquid to be poured in the mould county, places 55-90 ℃ of baking oven 10-60 minute formation support.
4. degeneration: support is immersed in taking-up after degeneration 10-15 hour in the denaturant solution (like ethanol),
Placed distilled water immersion 8-12 hours.
5. lyophilization: the support after the degeneration in-70 0C lyophilizations got final product in 1-5 hour the porous support material
Material.
The invention has the beneficial effects as follows:
1. the porous support materials that adopts gene recombination spider's thread protein to make is because its essence is natural egg
The white matter molecule makes it have good biocompatibility and degradability.
2. add biodegradable high polymer and not only further improve porous support mechanical performance, regulation and control bubble
The degradable characteristic of foam timbering material, and help reducing the cost of foam stand.
3. the processing technique of support is simple, and cost is low, also is fit to for industrialized mass.
Description of drawings
Fig. 1, Fig. 2 are foam stand material appearance figure.
Fig. 3, Fig. 4 are foam stand material profile sem photographs.
According to embodiment the present invention is further specified below.
The specific embodiment
EXAMPLE l
1. the preparation support prepares liquid: get 2. 25g gene recombination spider's thread protein powder and be dissolved in 7.5ml 98% formic acid and obtain 30% (w/v) protein solution, add the 0.45g polyvinyl alcohol again, and mixing, the support that promptly obtains containing gene recombination spider's thread protein and polyvinyl alcohol prepares liquid.
2. interpolation porogen: add 1. 5g porogen sodium chloride, mixing.
3. reverse mould, heating: the support that will be added with porogen prepares liquid to be poured in the cylindrical die (R=2cm), puts into 70 ℃ of baking ovens 30 minutes.
4. degeneration, distilled water immersion: support was immersed in the ethanol degeneration 15 hours, distilled water immersion 10 hours.
5. lyophilization: the support after the degeneration ,-70 ℃ got final product in freezing 2 hours porous support materials.
1. the preparation support prepares liquid: get 2. 5g gene recombination spider's thread protein powder and be dissolved in lOm1980h formic acid and obtain 25% (w/v) protein solution, add the 1.25g chitosan, mixing promptly obtains containing the gene recombination spider's thread egg
Support white and chitosan prepares liquid.
2. interpolation porogen: add 2.Og porogen sodium chloride, mixing.
3. reverse mould, heating: the support that will be added with porogen prepares liquid and pours rectangular die into
(4cmx3cmx3cm), put into 60 ℃ of baking ovens 20 minutes.
4. degeneration, distilled water immersion: support was immersed in the ethanol degeneration 10 hours, distilled water immersion 8 hours.
5. lyophilization: the support after the degeneration ,-70 ℃ got final product in freezing 4 hours porous support materials.
Embodiment 3
1. the preparation support prepares liquid: get 2g gene recombination spider's thread protein powder and be dissolved in 6m198% formic acid and obtain 33% (w/v) protein solution; Add 1.Og chitosan and 0.25g polyvinyl alcohol again; Mixing, the support that promptly obtains containing gene recombination spider's thread protein, chitosan and polyvinyl alcohol prepares liquid.
2. interpolation porogen: add 1. Og porogen sodium chloride, mixing.
3. reverse mould, heating: the support that will be added with porogen prepares liquid to be poured in the rectangular die (4cmx3cmx3cm), puts into 65 ℃ of baking ovens 30 minutes.
4. degeneration, distilled water immersion: support was immersed in the ethanol degeneration 13 hours, distilled water immersion 12 hours.
5. lyophilization: the support after the degeneration ,-70 ℃ got final product in freezing 3 hours porous support materials.
Claims (1)
1. the present invention relates to a kind of tissue engineered porous scaffold material, it is characterized in that obtaining porous support materials with gene recombination spider's thread protein and biodegradable high polymer blend,
(1). the protein of preparation porous support materials is gene recombination spider's thread protein;
(2). the high polymer of preparation porous support materials is biodegradable high polymer; Comprise polylactic acid (PLA), polyglycolic acid (PGA), polylactic acid and polyglycolic acid copolymer (PLGA), paracyanogen for acrylic ester, polycaprolactone, gather dioxanone and copolymer thereof, gather anhydride, poe, poly phosphazene, poly butyric, other linear aliphatic adoption esters, polyamino acid, chitin, chitosan, cellulose, polyvinyl alcohol, polyoxyethylene etc.
(3). the high polymer of preparation porous support materials can be wherein a kind of or their combination,
(4)., the proportioning of gene recombination spider's thread protein and biodegradable high polymer (mass ratio) is: the shared mass percent of gene recombination spider's thread protein is 55-95%, the shared mass percent of biodegradable high polymer is 45-50%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105534416A CN102475909A (en) | 2010-11-22 | 2010-11-22 | Porous scaffold material for tissue engineering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105534416A CN102475909A (en) | 2010-11-22 | 2010-11-22 | Porous scaffold material for tissue engineering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102475909A true CN102475909A (en) | 2012-05-30 |
Family
ID=46088766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105534416A Pending CN102475909A (en) | 2010-11-22 | 2010-11-22 | Porous scaffold material for tissue engineering |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102475909A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865090A (en) * | 2014-03-14 | 2014-06-18 | 山东理工大学 | Method for improving hydrophilia of polypeptide film by using polycaprolactone and polyethylene glycol |
| CN105088779A (en) * | 2015-08-05 | 2015-11-25 | 广州赛莱拉生物基因工程有限公司 | Collagen attachment membrane and preparation method thereof |
| WO2020183465A1 (en) * | 2019-03-11 | 2020-09-17 | Seevix Material Sciences Ltd. | Compositions comprising dragline spider silk |
| US12030919B2 (en) | 2016-02-11 | 2024-07-09 | Seevix Material Sciences Ltd. | Composite materials comprising synthetic dragline spider silk |
| US12453688B2 (en) | 2020-04-23 | 2025-10-28 | Seevix Material Sciences Ltd. | Cosmetic compositions comprising dragline spider silk |
-
2010
- 2010-11-22 CN CN2010105534416A patent/CN102475909A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865090A (en) * | 2014-03-14 | 2014-06-18 | 山东理工大学 | Method for improving hydrophilia of polypeptide film by using polycaprolactone and polyethylene glycol |
| CN103865090B (en) * | 2014-03-14 | 2016-04-27 | 山东理工大学 | A kind of method that polycaprolactone and polyoxyethylene glycol improve poly-peptide film wetting ability |
| CN105088779A (en) * | 2015-08-05 | 2015-11-25 | 广州赛莱拉生物基因工程有限公司 | Collagen attachment membrane and preparation method thereof |
| CN105088779B (en) * | 2015-08-05 | 2018-01-30 | 广州赛莱拉生物基因工程有限公司 | A kind of collagem membrane patch and preparation method thereof |
| US12030919B2 (en) | 2016-02-11 | 2024-07-09 | Seevix Material Sciences Ltd. | Composite materials comprising synthetic dragline spider silk |
| WO2020183465A1 (en) * | 2019-03-11 | 2020-09-17 | Seevix Material Sciences Ltd. | Compositions comprising dragline spider silk |
| US12453688B2 (en) | 2020-04-23 | 2025-10-28 | Seevix Material Sciences Ltd. | Cosmetic compositions comprising dragline spider silk |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Shen et al. | Engineering a highly biomimetic chitosan-based cartilage scaffold by using short fibers and a cartilage-decellularized matrix | |
| Sharma et al. | Biomaterials in tooth tissue engineering: a review | |
| CN110141687B (en) | A kind of gradient material for guiding periodontal hard and soft tissue regeneration and preparation method thereof | |
| Keane et al. | Biomaterials for tissue engineering applications | |
| Khang et al. | Biomaterials: tissue engineering and scaffolds | |
| CN106267341A (en) | One can organize induction bio-medical material | |
| CN102058902B (en) | Method for preparing mesh-shaped bionic bone porous stent material | |
| Wu et al. | Advancing scaffold‐assisted modality for in situ osteochondral regeneration: a shift from biodegradable to bioadaptable | |
| Bölgen et al. | Stem cell suspension injected HEMA-lactate-dextran cryogels for regeneration of critical sized bone defects | |
| CN101264341A (en) | Three-dimensional porous tissue engineering scaffold material, its preparation and application | |
| CN102475909A (en) | Porous scaffold material for tissue engineering | |
| CN102294049A (en) | Bioactive glass and chitosan composite bone repair material and preparation method and application thereof | |
| Lin et al. | Enhancing bone regeneration by combining mesenchymal stem cell sheets with β‐TCP/COL‐I scaffolds | |
| Wu et al. | Nature‐inspired strategies for the treatment of osteoarthritis | |
| CN114432492B (en) | A tissue engineering scaffold suitable for cartilage and its preparation method | |
| Morelli et al. | Polymers from renewable resources | |
| CN101249277A (en) | Three-dimensional porous tissue engineering scaffold material, preparation and application of its fiber bonding method | |
| Song et al. | In vitro extracellular matrix deposition by vascular smooth muscle cells grown in fibroin scaffolds, and the regulation of TGF-β1 | |
| CN109437826B (en) | Magnesium phosphate bone cement capable of being printed in 3D mode and preparation method and application thereof | |
| Sun et al. | Chirality‐induced bionic scaffolds in bone defects repair—a review | |
| Rondón et al. | Biomateriales utilizados en ingeniería de tejidos para la fabrica-ción de andamios Biomaterials used in tissue engineering for the manufacture of scaffolds | |
| Tao et al. | A simple and low-cost method to develop porous egg white scaffolds with controllable shape for cartilage regeneration | |
| CN100355468C (en) | Gene recombination spider's thread protein high polymer organizational engineering porous stent material | |
| CN108578780B (en) | A kind of preparation method of artificial bone scaffold with silver ion and mechanical gradient | |
| CN113368311A (en) | Hydroxyapatite/polyurethane porous bone repair material with shape memory |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120530 |