CN102405939A - Nematicidal composition containing paecilomyces lilacinus and carbosulfan - Google Patents
Nematicidal composition containing paecilomyces lilacinus and carbosulfan Download PDFInfo
- Publication number
- CN102405939A CN102405939A CN2011102895188A CN201110289518A CN102405939A CN 102405939 A CN102405939 A CN 102405939A CN 2011102895188 A CN2011102895188 A CN 2011102895188A CN 201110289518 A CN201110289518 A CN 201110289518A CN 102405939 A CN102405939 A CN 102405939A
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- CN
- China
- Prior art keywords
- paecilomyces lilacinus
- carbosulfan
- composition
- nematode
- root
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a nematicidal composition containing paecilomyces lilacinus and carbosulfan, which comprises paecilomyces lilacinus and carbosulfan, wherein the weight ratio of the paecilomyces lilacinus to the carbosulfan is 100-1000: 1-4, and the viable bacteria content of the paecilomyces lilacinus is 1-100 hundred million cfu/g. The two pesticides are mutually synergistic in the prevention and treatment of the nematode, and the chemical pesticide and the microbial pesticide act together. And realizes the function of simultaneously preventing and controlling the nematodes and other pests by one-time application of the medicament. The mixed use of the two pesticides greatly reduces the use amount of the single pesticide.
Description
Technical field
The invention belongs to agrochemical field, particularly relate to the nematicidal composition that comprises Paecilomyces lilacinus and carbosulfan.
Background technology
Nematode has another name called worm, belongs to invertebrate Nemathelminthes Nematoda, and the nematode hazard of plant symptom that is caused that causes harm is taken as Disease Processing over a long time.Compare with pathogenic microorganisms such as fungi, bacterium, viruses, pathogenic nematode has initiatively trend and thrusts the host, and shifts the characteristics that cause harm voluntarily with lancet.The causing harm of nematode, be not only and draw plant nutrition and cause that the underproduction and quality descend, and the plant roots cell transition is increased becomes goiter and tumor, loses the ability of absorption nutrient and moisture, and plant is declined extremely; In addition, causing harm of nematode, also can cause plant to suffer the attack of pathogen more easily and cause crop that disease takes place.
Though the control of nematodosis has obtained bigger progress, also there is certain problem.The consciousness that mainly shows integrated control is thin, puts undue emphasis on the importance of chemical pesticide, has ignored the reasonable utilization of other prophylactico-therapeutic measures; Monocrop continuous cropping, cropping index are high, or the unreasonable of chemical fertilizer use, and cause the trend that being of root knot nematode disease increase the weight of year by year, up to the present, form commercialization production and be widely used in the nematode biological prevention and control agent in land for growing field crops very few both at home and abroad.
The chemical agent that is used to prevent and treat root knot nematode disease has fumigation type, like Celfume, chloropicrin and D-D mixture etc.Also have; Like carbofuran, Aldicarb and Isofenphos methyl; A collection of chemical agents such as phonamiphos, Nemacur, gram line pellet, phoxim, Mi Leer and carbosulfan are processed into the nematicide formulation at present; And obtained good effect aspect preventing and treating in the root knot nematode disease of crops such as peanut, tobacco, sugarcane, cucumber, traditional Chinese medicine, at present, the nematocide of widespread usage also has the last ten thousand, metham-sodium, dazomet, lythidathion and dithiocyano-methane etc. both at home and abroad.
The bacterium that parasitizes root-knot nematode has Pasteurella,, puncture Pasteur bacillus, Pseudomonas fluorescence; Bacillus sphaericus, bacillus subtilis, bacillus thuringiensis,Bt, rhizosphere bacteria has preventive effect to Meloidogyne incognita; Can reduce the root footing, increase root system, it is heavy to increase root.
Paecilomyces lilacinus can effectively be prevented and treated root-knot nematode (Meloidogynespp.) and SCN (Heterodera sp.), to the ovum parasitic rate of Meloidogyne incognita up to 60%～70%.Paecilomyces lilacinus is is all prevented and treated usefulness to multiple nematode, and its host has root-knot nematode, SCN, golden nematode, golden nematode, is biological prevention and control agent (Amoncho etal, 1995 of the optimum future of control root-knot nematode; Angieri et al, 1997; Khan et al, 1988,1992,2000).Because this bacterium can adapt to different climatic conditions, and line eggs is had higher parasitic rate, can effectively control soil nematodes quantity, obviously reduce harm, be widely used in the production practices.At present, the nematologist of nearly 70 countries in the world prevents and treats plant nematodes such as root-knot nematode, SCN with it, and has obtained preventive effect preferably.
In recent years, Research in China person has also done a large amount of biological and ecological methods to prevent plant disease, pests, and erosion research to this bacterium, also confirms that this bacterium has higher parasitic rate to root-knot nematode, can effectively control the quantity of root-knot nematode in the soil, obviously reduces harm.Paecilomyces lilacinus has just reduced the illness that other pathogens of host plant cause to a certain extent to inhibition or the antagonism of microorganism on every side, better plays the biological and ecological methods to prevent plant disease, pests, and erosion effect.There is research to show that also this bacterium can also produce the metabolite of multiple biologically active.
Carbosulfan, english common name carbosulfan, chemical name: 2; 3-dihydro-2; To be that carbofuran is low poison derivative for 2-dimethyl benzofuran-7-base (dibutylamino sulphur) methyl carbamate, carbosulfan, is the inhibitor of cholinesterase; Have tag, stomach toxicity and systemic action, the various pests such as aphid, mite, wireworm, colorado potato beetle, tealeaves cicada, oriental fruit moth, apple skin worm that is used to prevent and treat crops such as oranges and tangerines, fruit tree, cotton, paddy rice.Various nematodes had the good activity of killing.Insecticidal spectrum is wider, and aphid, citrus rust mite etc. is all had very high killing effect; Instant effect promptly played a role after the dispenser in 20 minutes.But use consumption big when this agricultural chemicals exists independent nematicide too, effect is undesirable, and problems such as being easy to generate pesticide resistance is used in long-term single agent.
For realizing killing better various insects, the present invention is applied to crop control nematode and has obtained unforeseeable effect through carbosulfan and Paecilomyces lilacinus is composite.
Summary of the invention
For solving the problems of the technologies described above; The invention provides a kind of composition of preventing and treating nematode; Said composition comprises Paecilomyces lilacinus bacterium and carbosulfan, and the weight ratio of Paecilomyces lilacinus and carbosulfan is 100-1000: 1-4, and wherein the viable bacteria amount that contains of Paecilomyces lilacinus is 1-100 hundred million cfu/ gram.
Wherein 1-100 hundred million cfu/ gram is meant that generally the bacterial number alive in every gram medicine is hundred million of 1-100, or perhaps the quantity of living spores is hundred million of 1-100.
In the above-mentioned composition, the weight ratio of preferred Paecilomyces lilacinus and carbosulfan is 200-800: 1-3.
In the above-mentioned composition, the viable bacteria amount that contains of preferred Paecilomyces lilacinus is 1-10 hundred million cfu/ gram, especially is preferably 500,000,000 cfu/ gram.
Above-mentioned nematicidal composition of the present invention can also contain other insecticide or nematocide; For example Acetamiprid, acrinathrin, Aldicarb, α-cypermethrin, aluminum phosphate, Amitraz, nimbin, azinphos-methyl, Benfuracard micro, β-cyfloxylate, β-cypermethrin, Biphenthrin, borax, Brodifacoum, fenisobromolate, Buprofezin, Buprofezin, cadusafos, carbaryl, Padan's hydrochloride, bromine worm are clear, chlopyrifos, citronella oil, four mite piperazines, copper, coumatetralyl, cynock, cyfloxylate, plictran, cypermethrin, fly eradication amine, d_allethrin, dazomet, decis, demeton-methyl, diazinon, dichlorvos, dicofol, difenacoum, diflubenzuron, disulfoton, emaricin, 5a,6,9,9a-hexahydro-6,9-methano-2,4, sumicidin, phonamiphos, phonamiphos, ethylene dibromide, second mite azoles, fenamiphos, fenamiphos, fenazaquin, benzene fourth element, fenbutatin oxide, sumithion, fenoxycarb, fenpropathrin, azoles mite ester, fenthion, sumicidin, fluorine worm nitrile, flufenoxuron, flumethrin, lythidathion, Fumngillin, furfural, garlic extract, hydramethylnon, Imidacloprid, indenes worm prestige, λ-cyhalothrin, Acarus tritici urea, magnesium phosphide, mancozeb, maple lactone, malathion, the methaldehyde, metham-sodium, acephatemet, methidathion, methiocarb, Methomyl, Celfume, methyl-parathion, Menite, more visit rhzomorph, mineral oil, fluorine uride, omethoate, neighbour-phenylphenol, oxamyl, metilomerkaptofosoksid, parathion, permethrin, phenthoate dimephenthoate cidial, thimet, phosmet, phoxim, Aphox, vulcanize sulphur, fatty acid sylvite, Profenofos, alkynes mite spy, unden, protein hydrolysate, Toyodan, pyrethrin, pyrrole propyl ether, quinalphos, rapeseed oil, rotenone, silica-based repellent agent, prodan, pleocidin, spiral shell mite ester, sulphur, tartar emetic, taufluvalinate, worm hydrazides, Temefos, Terbufos, Ravap, tetradecene yl acetate, tetradiphon, thiophene worm quinoline, thiophene worm piperazine, the two prestige of sulphur more, fill in logical sequence, chlorophos, bell urea, spy lure ketone, cypermethrin, zinc phosphide etc. extremely; If possible contain a kind of in the above-mentioned insecticide incessantly, can contain more than one.The insecticide that adds other is generally the effect that strengthens composition kill pests of the present invention, on the other hand, also can strengthen the control efficiency of nematode.
Above-mentioned composition of the present invention is prepared into wetting powder, suspending agent, granule or microcapsule formulations.The method that the application above-mentioned composition prepares said preparation can be the common method preparation that those skilled in the art prepare preparation.
Line insect types that above-mentioned composition of the present invention can be used to kill such as plant nematode class, preferred, above-mentioned composition of the present invention can be used for killing tobacco root-knot nematode or paddy rice root-knot nematode.
The crop that above-mentioned composition of the present invention can be used to prevent and treat the nematodiasis invasion and attack has: can be used for the nematoda control of vegetable crop fruit trees such as soybean, beet, potato, wheat class, corn, paddy rice, cotton, peanut, banana, citrus, pineapple, tea shoot, sugarcane, sweet potato, tobacco, red bayberry, Momordica grosvenori, kiwi fruit, peach, tomato, capsicum, eggplant, persimmon, carrot, grape, strawberry, fig, cucumber, celery, pumpkin, muskmelon, watermelon, balsam pear, Kidney bean; Gymnospermous plants is like pine tree.
The purposes that the present invention also provides above-mentioned composition to be used to prevent and treat nematode, wherein said nematode are preferably tobacco root-knot nematode or paddy rice root-knot nematode.
Composition of the present invention can be used through root when nematoda control, and leaves and stems is used, and concrete application process such as liquid level spray, and root is mixed soil and sowed, and uses through filling root soluble in water etc.Amount of application is every mu of (667 square metres) 1-10kg, and preferred, amount of application is 1-5kg.
Preferably, the invention provides and use the method that above-mentioned composition is used to prevent and treat plant root-knot nematode, it is characterized in that composition mixed that soil is sowed or water-soluble filling root is applied to plant root soil, said crop is paddy rice or tobacco.
The beneficial effect of composition of the present invention is:
Composition of the present invention is developed for effectively preventing and treating nematode, can effectively prevent and treat nematode on the one hand, two kinds of mutual Synergistics of agricultural chemicals in the control of nematode, and chemical pesticide and microbial pesticide concur.In addition,, when the nematicide aspect strengthens the nematicide effect of Paecilomyces lilacinus, can also kill the insect of crop growth environment, realize that the applied once medicine prevents and treats the effect of nematode and other insects simultaneously because carbosulfan can also desinsection.The usage amount that greatly reduces independent agricultural chemicals is used in the mixing of two kinds of agricultural chemicals.
Embodiment
In order to understand the present invention, further specify the present invention with embodiment below, but do not limit the present invention.
Paecilomyces lilacinus among raw material sources: the embodiment 1 is 500,000,000 spore alive/g Paecilomyces lilacinus granules that Seoul Heilongjiang Qianger biochemical technology development Co., Ltd sells.The former medicine of carbosulfan is that the market purchase obtains.
Embodiment 1 Paecilomyces lilacinus and carbosulfan composition are to the preventive effect of paddy rice root-knot nematode
Use Paecilomyces lilacinus and carbosulfan and handle the paddy rice root-knot nematode; Comprise independent application control paddy rice root-knot nematode and two kinds of active ingredient mixed applying control paddy rice root-knot nematodes, application process is root soil to be irritated root use, and the concentration of Paecilomyces lilacinus agricultural chemicals is 500,000,000 living spores/grams; Application dosage is that the weight that every mu (667 square metres) are used is calculated; The carbosulfan application dosage was that every mu (667 square metres) weight of using is calculated, and carries out dispenser to the paddy rice root-knot nematode of identical nematodiasis, measured preventive effect at 24 hours; Repeated test 5 times is calculated through mean value.The value of preventive effect is meant that the percentage of killing nematode representes, according to the synergy situation of two kinds of medicaments of cobby formula clearing. specifically test retrieve data and see table.
| Composition | Amount of application/every mu | Theoretical preventive effect | Actual preventive effect | The synergy situation |
| Paecilomyces lilacinus | 1Kg | - | 53.69％ | - |
| Paecilomyces lilacinus | 4Kg | - | 76.24％ | - |
| Carbosulfan | 5g | - | 49.98％ | - |
| Carbosulfan | 15g | - | 85.85％ | - |
| Paecilomyces lilacinus+carbosulfan | 1Kg+5g | 76.84％ | 84.68％ | Synergistic |
| Paecilomyces lilacinus+carbosulfan | 1Kg+15g | 93.45％ | 100.00％ | Synergistic |
| Paecilomyces lilacinus+carbosulfan | 4Kg+5g | 88.16％ | 98.45％ | Synergistic |
| Paecilomyces lilacinus+carbosulfan | 4Kg+15g | 96.64％ | 100.00％ | Synergistic |
Find that through above-mentioned experiment Paecilomyces lilacinus has strengthened the independent preventive effect to the paddy rice root-knot nematode through mixing with carbosulfan.Embody the effect of Synergistic for the control of paddy rice root-knot nematode.The paddy rice of having used composition is on average increased production 2-3% than using Paecilomyces lilacinus separately and using the carbosulfan raising the output separately.The insect of rice root is answered and reduces, and total eradicative rate of other insects has reached more than 82%.。
Claims (7)
1. composition of preventing and treating nematode, said composition comprises Paecilomyces lilacinus bacterium and carbosulfan, and the weight ratio of Paecilomyces lilacinus and carbosulfan is 100-1000: 1-4, and wherein the viable bacteria amount that contains of Paecilomyces lilacinus is 1-100 hundred million cfu/ gram.
2. composition according to claim 1, wherein the weight ratio of Paecilomyces lilacinus and carbosulfan is 200-800: 1-3.
3. composition according to claim 1, wherein the viable bacteria amount that contains of Paecilomyces lilacinus is 1-10 hundred million cfu/ gram.
4. composition according to claim 1, wherein the viable bacteria amount that contains of Paecilomyces lilacinus is 500,000,000 cfu/ grams.
5. according to each described composition of claim 1-4, said composition is prepared into wetting powder, suspending agent, granule or microcapsule formulations.
6. each composition of the claim 1-4 purposes that is used to prevent and treat nematode, wherein said nematode is tobacco root-knot nematode or paddy rice root-knot nematode.
7. each composition of the claim 1-4 method that is used to prevent and treat plant root-knot nematode is characterized in that composition mixed that soil is sowed or water-soluble filling root is applied to plant root soil, and said crop is paddy rice or tobacco.
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| WO2014086749A3 (en) * | 2012-12-03 | 2014-08-28 | Bayer Cropscience Ag | Composition comprising a biological control agent and an insecticide |
| CN104818216A (en) * | 2015-01-20 | 2015-08-05 | 鲁东大学 | Paecilomyces lilacinus for prevention and control of meloidogyne diseases of tomato and grape |
| CN105875661A (en) * | 2016-04-29 | 2016-08-24 | 山东胜伟园林科技有限公司 | Biological pesticide containing Paecilomyces lilacinus for preventing and controlling soybean diseases and insect pests and preparation method thereof |
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| CN103789211B (en) * | 2012-10-31 | 2015-09-30 | 国家海洋局第三海洋研究所 | One strain has the abyssal sediment source Paecilomyces lilacinus A65 of anti-tealeaves pathomycete activity |
| CN103789211A (en) * | 2012-10-31 | 2014-05-14 | 国家海洋局第三海洋研究所 | Paecilomyces lilacinus A65 coming from deep-sea sediment and having anti-pathomycete activity for tea leaves |
| CN105025722A (en) * | 2012-12-03 | 2015-11-04 | 拜耳作物科学股份公司 | Compositions comprising biological control agents and pesticides |
| WO2014086749A3 (en) * | 2012-12-03 | 2014-08-28 | Bayer Cropscience Ag | Composition comprising a biological control agent and an insecticide |
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| CN104818216A (en) * | 2015-01-20 | 2015-08-05 | 鲁东大学 | Paecilomyces lilacinus for prevention and control of meloidogyne diseases of tomato and grape |
| CN104818216B (en) * | 2015-01-20 | 2018-01-16 | 鲁东大学 | One plant is used to prevent and treat tomato and the Paecilomyces lilacinus of grape root knot nematode disease evil |
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