CN102139934A - Method for removing heavy metal ions from aqueous solution by utilizing waste bacterial packets from industrial production of flammulina velutipes - Google Patents
Method for removing heavy metal ions from aqueous solution by utilizing waste bacterial packets from industrial production of flammulina velutipes Download PDFInfo
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- CN102139934A CN102139934A CN2011100276234A CN201110027623A CN102139934A CN 102139934 A CN102139934 A CN 102139934A CN 2011100276234 A CN2011100276234 A CN 2011100276234A CN 201110027623 A CN201110027623 A CN 201110027623A CN 102139934 A CN102139934 A CN 102139934A
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- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 48
- 150000002500 ions Chemical class 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000002699 waste material Substances 0.000 title claims abstract description 21
- 239000007864 aqueous solution Substances 0.000 title claims abstract description 19
- 238000009776 industrial production Methods 0.000 title claims abstract description 5
- 240000006499 Flammulina velutipes Species 0.000 title claims abstract 9
- 235000016640 Flammulina velutipes Nutrition 0.000 title claims abstract 9
- 230000001580 bacterial effect Effects 0.000 title abstract description 5
- 239000000243 solution Substances 0.000 claims abstract description 16
- 239000002245 particle Substances 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 58
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 39
- 238000004519 manufacturing process Methods 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims 2
- 235000012343 cottonseed oil Nutrition 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 238000003306 harvesting Methods 0.000 claims 1
- 238000001179 sorption measurement Methods 0.000 abstract description 43
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 abstract description 12
- 229910052759 nickel Inorganic materials 0.000 abstract description 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052793 cadmium Inorganic materials 0.000 abstract description 4
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052725 zinc Inorganic materials 0.000 abstract description 4
- 239000011701 zinc Substances 0.000 abstract description 4
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 abstract description 2
- 239000003463 adsorbent Substances 0.000 abstract 1
- QUQFTIVBFKLPCL-UHFFFAOYSA-L copper;2-amino-3-[(2-amino-2-carboxylatoethyl)disulfanyl]propanoate Chemical compound [Cu+2].[O-]C(=O)C(N)CSSCC(N)C([O-])=O QUQFTIVBFKLPCL-UHFFFAOYSA-L 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 description 32
- 238000010923 batch production Methods 0.000 description 24
- 239000006228 supernatant Substances 0.000 description 18
- 238000010521 absorption reaction Methods 0.000 description 16
- 229920000742 Cotton Polymers 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 238000000703 high-speed centrifugation Methods 0.000 description 6
- 239000002594 sorbent Substances 0.000 description 6
- 239000010949 copper Substances 0.000 description 5
- 239000011133 lead Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000002351 wastewater Substances 0.000 description 5
- 235000015099 wheat brans Nutrition 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 230000000274 adsorptive effect Effects 0.000 description 4
- 239000011572 manganese Substances 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 240000005710 Auricularia polytricha Species 0.000 description 2
- 235000000024 Auricularia polytricha Nutrition 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000009388 chemical precipitation Methods 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 229910052748 manganese Inorganic materials 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000001172 regenerating effect Effects 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 238000001223 reverse osmosis Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- 239000010938 white gold Substances 0.000 description 2
- 229910000832 white gold Inorganic materials 0.000 description 2
- 241000609240 Ambelania acida Species 0.000 description 1
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 244000060011 Cocos nucifera Species 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- 241001537207 Flammulina Species 0.000 description 1
- 241001123597 Funneliformis mosseae Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000222540 Gomphidius Species 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 241000080590 Niso Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000222385 Phanerochaete Species 0.000 description 1
- 241000222393 Phanerochaete chrysosporium Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 235000021438 curry Nutrition 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000002921 fermentation waste Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/30—Wastewater or sewage treatment systems using renewable energies
- Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
Landscapes
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
本发明涉及了一种利用金针菇工厂化生产废菌包去除水溶液中重金属离子的方法，其特征在于将金针菇工厂化生产废弃的菌包自然晒干，后烘至恒重；粉碎后过筛得425～600μm的颗粒作为生物吸附剂；称取一定量的生物吸附剂装入盛有重金属溶液的三角锥形瓶中，生物吸附剂用量为6～10g/L，在23～27℃，100～150r/min条件下置于恒温振荡器中振荡吸附3～5h，该吸附剂对水溶液中8～12mg/L的镉和铅的去除率达95以上；对40～60mg/L的锰、镍、铜、锌的去除率分别为80～85％、65～70％、70～75％、75～80％。本发明的生物吸附剂具有成本低，吸附效率高，工艺简单，操作方便的优点，将有广阔的应用前景。The invention relates to a method for removing heavy metal ions in an aqueous solution by utilizing the waste bacterial packs produced by the industrial production of Flammulina velutipes, which is characterized in that the discarded bacterial packs of Flammulina velutipes are naturally dried, and then baked to constant weight; after being crushed, sieved to obtain 425 Particles of ~600μm are used as biosorbent; weigh a certain amount of biosorbent and put it into a conical flask filled with heavy metal solution. Placed in a constant temperature oscillator for oscillating adsorption for 3-5 hours under the condition of /min, the removal rate of the adsorbent for 8-12mg/L cadmium and lead in aqueous solution is above 95; for 40-60mg/L manganese, nickel, copper , Zinc removal rates were 80-85%, 65-70%, 70-75%, 75-80%. The biosorbent of the invention has the advantages of low cost, high adsorption efficiency, simple process and convenient operation, and will have broad application prospects.
Description
Technical field:
The present invention relates to a kind of method of utilizing biomaterial to remove heavy metal ion in the aqueous solution, be specifically related to a kind of method of utilizing useless bacterium bag in the needle mushroom batch production production process to remove heavy metal ion such as cadmium in the aqueous solution, lead, manganese, nickel, copper, zinc as biological adsorption agent.
Background technology:
How administering heavy metal polluted waste water is the outstanding problem that current environmental protection work faces.At present aspect the heavy metal wastewater thereby of handling lower concentration, mainly contain chemical precipitation method, ion exchange method, chemistry redox method, reverse osmosis method, ultra-filtration method, active carbon absorption technology etc., its cost height, low, the generation secondary pollution of efficient, a little less than the regenerative power, regeneration back adsorptive power does not have obvious raising.Biosorption process particularly aspect the heavy metal wastewater thereby of handling lower concentration, has very wide prospect as a kind of emerging treatment technology.Compare with traditional treatment method (chemical precipitation method, ion exchange method, chemistry redox method, reverse osmosis method, ultra-filtration method, active carbon absorption technology etc.), the biosorption process cost is low, efficient is high, do not produce secondary pollution, regenerative power is strong, and regeneration back adsorptive power does not have obvious reduction.
Radicula byssoidea shows the ability of very strong absorption heavy metal.The mycelium of Auricularia polytricha (Mout) Sacc. (Auriculariapolytricha), Phanerochaete chrysosporium (Phanerochaete chrysosporiu), red suede lid bolete (Gomphidiusvi scidus), rivet mushroom (Gomphidius viscidus), bush mycorrhizal fungi (Glomus mosseae) etc. all can be removed the heavy metal in the aqueous solution effectively.The industrial fermentation waste material black-koji mould filament of inactivation has very strong adsorptive power to the nickel in the aqueous solution, calcium, iron and chromium.Agricultural waste materials, especially rich cellulose, have very strong biological adsorptive power.Rice bran, stalk, wheat bran, wheat husk, Pericarppium arachidis hypogaeae, soybean peel, Exocarpium cocois (Cocos nucifera L), nut-shell, cotton seed hulls, useless tealeaves, corn cob, bagasse, Pericarpium Mali pumilae, beet pulp, Sunflower Receptacle stem, cotton straw etc. are all attempted being used to adsorb heavy metal.
Edible fungus industrial production has become the direction of China's mushroom industry development.The Edible Fungus's Industrialized Production in China kind mainly is white gold needle mushroom (Flammulina velutiper) at present, and its turnout accounts for more than 60% of all edible fungus industrial products.5 tons white gold needle mushroom factory of a daily output just can produce the useless bacterium of about 5-6 ton every day and wrap, and quantum of output is huge.Therefore, the processing of useless bacterium bag is a problem of needing solution badly.At present, the needle mushroom batch production is produced useless bacterium bag and is mainly used to do fertilizer, fodder additives, biogas fermentation raw material or is used for planting other mushroom again, does not see the report used as the heavy metal ion biological adsorption agent.
Batch production is produced needle mushroom and is only gone out a damp mushroom, these the culture material of cotton seed hulls, wheat bran and corn cob in the useless bacterium bag after comprising fermentation, also contain a large amount of golden mushroom myceliums, specific surface area is huge, but the functional group of containing multiple bonding heavy metal ion, these all make it become possibility as biological adsorption agent.
The new purposes of the useless bacterium bag of exploitation Biosorbent for heavy metals to handle heavy metal-containing waste water, is " treatment of wastes with processes of wastes against one another ", " turning waste into wealth ", and the recycling that wraps for the processing and the useless bacterium of heavy metal-containing waste water provides new approach.This measure has taken into account environment and economic benefit, will have broad application prospects!
Summary of the invention:
The object of the present invention is to provide a kind of cost low, the adsorption efficiency height, technology is simple, and the easy to operate useless bacterium bag that utilizes the needle mushroom batch production to produce is removed the method for heavy metal ion in the aqueous solution.
Purpose of the present invention can reach by following measure: a kind of method of utilizing the needle mushroom batch production to produce heavy metal ion in the useless bacterium bag removal aqueous solution is characterized in that comprising the steps:
(1), useless bacterium bag nature that the needle mushroom batch production is produced dries, after put into 55～65 ℃ loft drier and dry to constant weight;
(2), the useless bacterium bag that will dry pulverizes the back and crosses standard sieve, the particle that obtains 425～600 μ m is loaded in the valve bag as biological adsorption agent;
(3), above-mentioned biological adsorption agent is put into 55～65 ℃ of loft drier and dried by the fire 20～30h; After pack in the triangular pyramidal bottle that fills heavy metal solution, biological adsorption agent consumption 6～10g/L at 23～27 ℃, places constant temperature oscillator vibration absorption 3～5h under 100～150r/min condition.
In order further to realize purpose of the present invention, the useless bacterium bag that described needle mushroom batch production is produced is meant by cotton seed hulls, wheat bran, corn cob according to 1: 1: 1 ratio, add the water mixing by material-water ratio 1: 1.2～1.5 and make substratum, pack, normal pressure moist heat sterilization 10-12h is cooled to room temperature, inoculation needle mushroom bacterial classification, the bacterium and the first damp fruiting process through 35～45 days, the useless bacterium bag of depleted after the needle mushroom of gathering.
The present invention can produce following positively effect compared with the prior art:
1, batch production production needle mushroom only goes out a damp mushroom, these culture materials of cotton seed hulls, wheat bran and corn cob in the useless bacterium bag after comprising fermentation, also contain a large amount of golden mushroom myceliums, useless bacterium bag specific surface area is huge, but the functional group of containing multiple bonding heavy metal ion, the adsorption efficiency height, the cadmium of 8～12mg/L and plumbous clearance reach more than 95% in testing the aqueous solution; Clearance to the manganese of 40～60mg/L, nickel, copper, zinc is respectively 80～85%, 65～70%, 70～75%, 75～80%.
2, useless bacterium bag biological adsorption agent is produced in the needle mushroom batch production, the adsorption efficiency height, and technology is simple, and is easy to operate, and cost is low.
3, this method has promptly been widened the range of choice of biological adsorption agent, has made full use of the agriculture production waste again---and useless bacterium bag is produced in the needle mushroom batch production, is " treatment of wastes with processes of wastes against one another ", " turning waste into wealth ".This measure has taken into account environment and economic benefit, will have broad application prospects!
Embodiment:
Below the specific embodiment of the present invention is elaborated:
Raw material is prepared: the needle mushroom batch production is produced useless bacterium bag from Shandong Province highly dense curry favour moral agricultural-food company limited, be meant by cotton seed hulls, wheat bran, corn cob according to 1: 1: 1 ratio, add the water mixing by material-water ratio 1: 1.2～1.5 and make substratum, pack, normal pressure moist heat sterilization 10-12h is cooled to room temperature, inoculation needle mushroom bacterial classification, the bacterium and the first damp fruiting process through 35～45 days, the useless bacterium bag of depleted after the needle mushroom of gathering.From the useless bacterium bag of the needle mushroom of other producer, also can adopt as long as the culture material composition is the same.
Embodiment 1:
1, the needle mushroom batch production is produced useless bacterium bag and dries naturally earlier, after put into 55～65 ℃ loft drier and dry to constant weight;
2, the useless bacterium bag that will dry is pulverized the back and is crossed standard sieve, and the particle that obtains 425～600 μ m is loaded in the valve bag as biological adsorption agent.
3, above-mentioned biological adsorption agent is put into 55～65 ℃ of loft drier and dried by the fire 20～30h; The 0.3g waste bacterium bag grain packed into to be filled in the 150ml triangular pyramidal bottle of 50ml, pH=6,10mg/LCd (NO3) 24H2O solution, and promptly the sorbent material consumption is 6g/L, 23 ℃, places constant temperature oscillator vibration absorption 4h under the 120r/min condition.Afterwards, high speed centrifugation is got supernatant liquor, Cd in the supernatant liquor
2+Concentration measure with flame atomic absorption spectrophotometer (GBC932-AA).Useless bacterium bag biological adsorption agent is to Cd
2+Clearance Re calculate by following formula:
In the formula, R
eClearance for heavy metal ion; C
0Initial concentration (mg/L) for heavy metal ion in the solution; C
eConcentration (mg/L) for heavy metal ion in the supernatant liquor after the adsorption equilibrium.
As calculated, heavy metal ion biological adsorption agent provided by the invention---the useless bacterium bag of needle mushroom batch production production reaches 96.5% to the clearance of the cadmium of 10mg/L in the aqueous solution.
Embodiment 2:
1, the needle mushroom batch production is produced useless bacterium bag and dries naturally earlier, after put into 55～65 ℃ loft drier and dry to constant weight;
2, the useless bacterium bag that will dry is pulverized the back and is crossed standard sieve, and the particle that obtains 425～600 μ m is loaded in the valve bag as biological adsorption agent.
3, above-mentioned biological adsorption agent is put into 55～65 ℃ of loft drier and dried by the fire 20～30h; The 0.4g waste bacterium bag grain packed into fill 50ml, pH=5,10mg/L Pb (NO
3)
2In the 150ml triangular pyramidal bottle of solution, promptly the sorbent material consumption is 8g/L, 25 ℃, places constant temperature oscillator vibration absorption 3h under the 120r/min condition.Afterwards, high speed centrifugation is got supernatant liquor, Pb in the supernatant liquor
2+Concentration measure with flame atomic absorption spectrophotometer (GBC932-AA).Useless bacterium bag biological adsorption agent is to Pb
2+Clearance R
eCalculate by following formula:
In the formula, R
eClearance for heavy metal ion; C
0Initial concentration (mg/L) for heavy metal ion in the solution; C
eConcentration (mg/L) for heavy metal ion in the supernatant liquor after the adsorption equilibrium.
As calculated, heavy metal ion biological adsorption agent provided by the invention---the useless bacterium bag of needle mushroom batch production production can reach 95.9% to the clearance of the lead of 10mg/L in the aqueous solution.
Embodiment 3:
1, the needle mushroom batch production is produced useless bacterium bag and dries naturally earlier, after put into 55～65 ℃ loft drier and dry to constant weight;
2, the useless bacterium bag that will dry is pulverized the back and is crossed standard sieve, and the particle that obtains 425～600 μ m is loaded in the valve bag as biological adsorption agent.
3, above-mentioned biological adsorption agent is put into 55～65 ℃ of loft drier and dried by the fire 20～30h; The 0.5g waste bacterium bag grain packed into fill 50ml, pH=5,50mg/L MnSO
4H
2In the 150ml triangular pyramidal bottle of O solution, promptly the sorbent material consumption is 10g/L, 25 ℃, places constant temperature oscillator vibration absorption 5h under the 120r/min condition.Afterwards, high speed centrifugation is got supernatant liquor, Mn in the supernatant liquor
2+Concentration measure with flame atomic absorption spectrophotometer (GBC932-AA).Useless bacterium bag biological adsorption agent is to Mn
2+Clearance R
eCalculate by following formula:
In the formula, R
eClearance for heavy metal ion; C
0Initial concentration (mg/L) for heavy metal ion in the solution; C
eConcentration (mg/L) for heavy metal ion in the supernatant liquor after the adsorption equilibrium.
As calculated, heavy metal ion biological adsorption agent provided by the invention---the useless bacterium bag of needle mushroom batch production production is 81.2% to the clearance of the manganese of 50mg/L in the aqueous solution.
Embodiment 4:
1, the needle mushroom batch production is produced useless bacterium bag and dries naturally earlier, after put into 55～65 ℃ loft drier and dry to constant weight;
2, the useless bacterium bag that will dry is pulverized the back and is crossed standard sieve, and the particle that obtains 425～600 μ m is loaded in the valve bag as biological adsorption agent.
3, above-mentioned biological adsorption agent is put into 55～65 ℃ of loft drier and dried by the fire 20～30h; The 0.4g waste bacterium bag grain packed into fill 50ml, pH=5,50mg/L NiSO
46H
2In the 150ml triangular pyramidal bottle of O solution, promptly the sorbent material consumption is 8g/L, 27 ℃, place constant temperature oscillator vibration absorption 4h under the 120r/min condition, and each handles triplicate.Afterwards, high speed centrifugation is got supernatant liquor, Ni in the supernatant liquor
2+Concentration measure with flame atomic absorption spectrophotometer (GBC932-AA).Useless bacterium bag biological adsorption agent is to Ni
2+Clearance R
eCalculate by following formula:
In the formula, R
eClearance for heavy metal ion; C
0Initial concentration (mg/L) for heavy metal ion in the solution; C
eConcentration (mg/L) for heavy metal ion in the supernatant liquor after the adsorption equilibrium.As calculated, heavy metal ion biological adsorption agent provided by the invention---the useless bacterium bag of needle mushroom batch production production is 68.7% to the clearance of the nickel of 50mg/L in the aqueous solution.
Embodiment 5:
1, the needle mushroom batch production is produced useless bacterium bag and dries naturally earlier, after put into 55～65 ℃ loft drier and dry to constant weight;
2, the useless bacterium bag that will dry is pulverized the back and is crossed standard sieve, and the particle that obtains 425～600 μ m is loaded in the valve bag as biological adsorption agent.
3, above-mentioned biological adsorption agent is put into 55～65 ℃ of loft drier and dried by the fire 20～30h; The 0.45g waste bacterium bag grain packed into fill 50ml, pH=5,50mg/L CuSO
45H
2In the 150ml triangular pyramidal bottle of O solution, promptly the sorbent material consumption is 9g/L, 25 ℃, place constant temperature oscillator vibration absorption 4h under the 120r/min condition, and each handles triplicate.Afterwards, high speed centrifugation is got supernatant liquor, Cu in the supernatant liquor
2+Concentration measure with flame atomic absorption spectrophotometer (GBC932-AA).Useless bacterium bag biological adsorption agent is to Cu
2+Clearance R
eCalculate by following formula:
In the formula, Re is the clearance of heavy metal ion; C0 is the initial concentration (mg/L) of heavy metal ion in the solution; Ce is the concentration (mg/L) of heavy metal ion in the supernatant liquor after the adsorption equilibrium.
As calculated, heavy metal ion biological adsorption agent provided by the invention---the useless bacterium bag of needle mushroom batch production production is 72.3% to the clearance of the copper of 50mg/L in the aqueous solution.
Embodiment 6:
1, the needle mushroom batch production is produced useless bacterium bag and dries naturally earlier, after put into 55～65 ℃ loft drier and dry to constant weight;
2, the useless bacterium bag that will dry is pulverized the back and is crossed standard sieve, and the particle that obtains 425～600 μ m is done
For biological adsorption agent is loaded in the valve bag.
3, above-mentioned biological adsorption agent is put into 55～65 ℃ of loft drier and dried by the fire 20～30h; The 0.35g waste bacterium bag grain packed into fill in the 150ml triangular pyramidal bottle of 50ml, pH=5,50mg/L ZnSO47H2O solution, be that the sorbent material consumption is 7g/L, 25 ℃, place constant temperature oscillator vibration absorption 4h under the 120r/min condition, each handles triplicate.Afterwards, high speed centrifugation is got supernatant liquor, and the concentration of Zn2+ is measured with flame atomic absorption spectrophotometer (GBC932-AA) in the supernatant liquor.Useless bacterium bag biological adsorption agent calculates by following formula the clearance Re of Zn2+:
In the formula, R
eClearance for heavy metal ion; C
0Initial concentration (mg/L) for heavy metal ion in the solution; C
eConcentration (mg/L) for heavy metal ion in the supernatant liquor after the adsorption equilibrium.As calculated, heavy metal ion biological adsorption agent provided by the invention---the useless bacterium bag of needle mushroom batch production production is 76.3% to the clearance of the zinc of 50mg/L in the aqueous solution.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110027623A CN102139934B (en) | 2011-01-21 | 2011-01-21 | A method for removing heavy metal ions in aqueous solution by utilizing the industrial production waste bacteria bag of Flammulina velutipes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110027623A CN102139934B (en) | 2011-01-21 | 2011-01-21 | A method for removing heavy metal ions in aqueous solution by utilizing the industrial production waste bacteria bag of Flammulina velutipes |
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| Publication Number | Publication Date |
|---|---|
| CN102139934A true CN102139934A (en) | 2011-08-03 |
| CN102139934B CN102139934B (en) | 2012-10-17 |
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| CN201110027623A Expired - Fee Related CN102139934B (en) | 2011-01-21 | 2011-01-21 | A method for removing heavy metal ions in aqueous solution by utilizing the industrial production waste bacteria bag of Flammulina velutipes |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103521193A (en) * | 2013-10-25 | 2014-01-22 | 鲁东大学 | Method for improving adsorption capacity of enoki mushroom industrialized production waste bacteria bags to heavy metals |
| CN107088399A (en) * | 2017-05-25 | 2017-08-25 | 东北农业大学 | A kind of preparation method and applications of germ bran biological carbon adsorbent |
| CN108046854A (en) * | 2018-01-06 | 2018-05-18 | 天津大学 | Mushroom bran for heavy metal adsorption-bacterial manure fermentation recycles coupling device and method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002018484A (en) * | 2000-07-10 | 2002-01-22 | Idemitsu Kosan Co Ltd | Treatment method for wastewater containing hardly decomposable substances |
| CN1541510A (en) * | 2003-04-28 | 2004-11-03 | 上海浦东天厨菇业有限公司 | Culture medium formula of factory run golden mushroom and its preparation process |
| CN1657429A (en) * | 2005-01-28 | 2005-08-24 | 湖南大学 | Method for removing cadmium-containing industrial wastewater by using mushroom culture medium waste |
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2011
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002018484A (en) * | 2000-07-10 | 2002-01-22 | Idemitsu Kosan Co Ltd | Treatment method for wastewater containing hardly decomposable substances |
| CN1541510A (en) * | 2003-04-28 | 2004-11-03 | 上海浦东天厨菇业有限公司 | Culture medium formula of factory run golden mushroom and its preparation process |
| CN1657429A (en) * | 2005-01-28 | 2005-08-24 | 湖南大学 | Method for removing cadmium-containing industrial wastewater by using mushroom culture medium waste |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103521193A (en) * | 2013-10-25 | 2014-01-22 | 鲁东大学 | Method for improving adsorption capacity of enoki mushroom industrialized production waste bacteria bags to heavy metals |
| CN107088399A (en) * | 2017-05-25 | 2017-08-25 | 东北农业大学 | A kind of preparation method and applications of germ bran biological carbon adsorbent |
| CN108046854A (en) * | 2018-01-06 | 2018-05-18 | 天津大学 | Mushroom bran for heavy metal adsorption-bacterial manure fermentation recycles coupling device and method |
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| CN102139934B (en) | 2012-10-17 |
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