CN102007855A - Method for grafting melons on double rootstocks - Google Patents
Method for grafting melons on double rootstocks Download PDFInfo
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- 238000009331 sowing Methods 0.000 claims abstract description 8
- 241000353135 Psenopsis anomala Species 0.000 claims abstract description 7
- 244000241257 Cucumis melo Species 0.000 claims description 42
- 239000000758 substrate Substances 0.000 claims description 18
- 240000008067 Cucumis sativus Species 0.000 claims description 14
- 239000003415 peat Substances 0.000 claims description 12
- 239000002985 plastic film Substances 0.000 claims description 12
- 229920006255 plastic film Polymers 0.000 claims description 12
- 239000002689 soil Substances 0.000 claims description 12
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 11
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 claims description 6
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 claims description 4
- 229910052902 vermiculite Inorganic materials 0.000 claims description 4
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- 235000009849 Cucumis sativus Nutrition 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 2
- 244000241235 Citrullus lanatus Species 0.000 claims 1
- 241000219122 Cucurbita Species 0.000 abstract description 4
- 238000003032 molecular docking Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 7
- 241000219109 Citrullus Species 0.000 description 5
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- 208000035240 Disease Resistance Diseases 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
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- 241000223218 Fusarium Species 0.000 description 2
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- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
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Abstract
本发明公开了一种瓜类双砧木嫁接方法,包括如下步骤:(1)将砧木白籽南瓜和黑籽南瓜成对同时播种;(2)将瓜类种子在砧木播种5-8天时播种;(3)当砧木白籽南瓜第一真叶的直径达4.5cm-6cm时,将白籽南瓜去除第一真叶,黑籽南瓜去除生长点,利用靠接法将砧木嫁接在一起;同时,将瓜类幼苗在下胚轴离子叶1.5cm-2.5cm处用刀片斜切,将切下的瓜类接穗用嫁接针斜插入白籽南瓜的下胚轴离子叶0.3cm-0.7cm位置处;(4)育苗管理,2-4天后定植。本发明将两种砧木的靠接及将瓜类接穗与砧木的插接同时进行,极大提高了操作效率,并大大降低了随之产生的管理成本。非常适用于嫁接育苗生产的工厂化。The invention discloses a method for grafting melons with double rootstocks, comprising the following steps: (1) sowing white-seeded pumpkins and black-seeded pumpkins in pairs at the same time; (2) sowing melon seeds 5-8 days after sowing the rootstocks; (3) When the diameter of the first true leaf of the stock white-seed pumpkin reaches 4.5cm-6cm, the first true leaf of the white-seed pumpkin is removed, and the growth point of the black-seed pumpkin is removed, and the rootstock is grafted together by the abutment method; meanwhile, The melon seedlings are obliquely cut with a blade at the hypocotyl ion leaf 1.5cm-2.5cm, and the cut melon scion is obliquely inserted into the hypocotyl ion leaf 0.3cm-0.7cm position of the white-seeded pumpkin with a grafting needle; ( 4) Seedling management, planting after 2-4 days. The present invention carries out the docking of two kinds of rootstocks and the plugging of melon scions and rootstocks at the same time, which greatly improves the operation efficiency and greatly reduces the management cost produced accordingly. It is very suitable for factory production of grafted seedlings.
Description
技术领域technical field
本发明涉及农作物栽培技术,特别是涉及一种瓜类嫁接方法。The invention relates to crop cultivation techniques, in particular to a melon grafting method.
背景技术Background technique
葫芦科瓜类作物是大众喜爱的食物。随着种植业结构的调整和对设施农业扶持力度的加大,瓜类作物保护地栽培面积逐年上升,产量与经济效益明显提高,对农业增效、农民增收发挥了积极作用。但是瓜类作物重茬种植导致枯萎病、蔓枯病等病害的严重发生,制约了瓜类作物的种植和发展。目前,嫁接栽培是生产上用来防治瓜类作物枯萎病等土传病害最有效的方法,白籽南瓜由于亲和性好,对瓜类作物品质影响相对较小,被普遍用作砧木。它比自根瓜类作物明显表现出产量高和抗病性好的特点。但是,随着嫁接栽培技术的普及及推广应用,在生产中又出现的新问题是,由于连作障碍的日益严重,以及新的致病生理小种的产生,在一些地区使用单一砧木的嫁接苗也会表现出严重的病害发生,而且这种现象日趋严重。另外用白籽南瓜嫁接的植株,在田间生产中还常常表现出早衰,亦严重影响了瓜类作物的产量和品质。如果改用其它砧木如黑籽南瓜虽然能表现出很好的抗病性和生长势,但对瓜类作物的品质风味影响很大,大大降低了其商品价值。Cucurbitaceae melon crops are popular foods. With the adjustment of the planting industry structure and the increase of support for facility agriculture, the cultivated area of melon crops has increased year by year, and the output and economic benefits have increased significantly, which has played a positive role in increasing agricultural efficiency and farmers' income. However, the repeated cropping of melon crops leads to the serious occurrence of diseases such as Fusarium wilt and creeping blight, which restricts the cultivation and development of melon crops. At present, grafting cultivation is the most effective method for preventing and controlling soil-borne diseases such as fusarium wilt of melon crops in production. White-seeded pumpkin is widely used as a rootstock because of its good compatibility and relatively little impact on the quality of melon crops. Compared with self-root melon crops, it obviously shows the characteristics of high yield and good disease resistance. However, with the popularization and application of grafting cultivation techniques, new problems have emerged in production. Due to the increasingly serious obstacles to continuous cropping and the emergence of new pathogenic physiological races, grafted seedlings with a single rootstock are used in some areas. It will also show serious disease occurrence, and this phenomenon is becoming more and more serious. In addition, the plants grafted with white-seeded pumpkin often show premature aging in field production, which also seriously affects the yield and quality of melon crops. If other rootstocks such as black seed pumpkin can be used instead to show good disease resistance and growth vigor, it has a great impact on the quality and flavor of melon crops, greatly reducing its commercial value.
发明内容Contents of the invention
本发明的目的是克服现有技术存在的不足,提供一种瓜类双砧木嫁接方法。The purpose of the invention is to overcome the deficiencies in the prior art and provide a method for grafting melons with double rootstocks.
本发明的技术方案概述如下:Technical scheme of the present invention is summarized as follows:
一种瓜类双砧木嫁接方法,包括如下步骤:A kind of melon double stock grafting method, comprises the steps:
一种瓜类双砧木嫁接方法,包括如下步骤:A kind of melon double stock grafting method, comprises the steps:
(1)将砧木白籽南瓜和黑籽南瓜相距1cm-1.5cm成对同时播种在育苗基质中;(1) rootstock white-seed pumpkin and black-seed pumpkin are sowed in pairs simultaneously in the seedling-raising substrate at a distance of 1cm-1.5cm;
(2)将瓜类种子在所述砧木播种5-8天时播种于装有育苗基质的育苗盘中;(2) melon seeds are sown in a seedling tray equipped with a seedling substrate when the rootstock is sown for 5-8 days;
(3)当所述砧木白籽南瓜第一真叶的直径达4.5cm-6cm时,将白籽南瓜去除第一真叶,黑籽南瓜去除生长点,利用靠接法将所述砧木白籽南瓜和黑籽南瓜嫁接在一起,用夹子固定;同时,将两片子叶展开角度为90-150度的瓜类幼苗在下胚轴离子叶1.5cm-2.5cm处用刀片斜切,去除根部,将切下的瓜类接穗用嫁接针斜插入白籽南瓜的下胚轴离子叶0.3cm-0.7cm位置处;(3) When the diameter of the first true leaf of the rootstock white seeded pumpkin reaches 4.5cm-6cm, the first true leaf of the white seeded pumpkin is removed, and the growth point of the black seeded pumpkin is removed, and the rootstock white seeded Pumpkin and black-seeded pumpkin are grafted together and fixed with clips; at the same time, two melon seedlings with cotyledons at an angle of 90-150 degrees are cut obliquely with a blade at 1.5cm-2.5cm of the hypocotyl ion leaf, and the roots are removed. The cut melon scion is obliquely inserted into the hypocotyl ion leaf 0.3cm-0.7cm position of the white-seeded pumpkin with a grafting needle;
(4)在25-30℃,用地膜直接覆盖在嫁接苗上以保证空气相对湿度为100%,同时用遮阳网覆盖避光,保持22-26小时,去除地膜,支起拱棚,改用塑料薄膜覆盖,使空气相对湿度为100%,继续用遮阳网覆盖避光45-50小时,去除遮阳网,按正常育苗管理5-15天,去除白籽南瓜第二真叶及生长点,按正常育苗管理,2-4天后定植。(4) At 25-30°C, cover the grafted seedlings directly with plastic film to ensure that the relative humidity of the air is 100%. Cover with film so that the relative air humidity is 100%, continue to cover with sunshade nets to avoid light for 45-50 hours, remove the sunshade nets, manage for 5-15 days according to normal seedling management, remove the second true leaves and growth points of white seeded pumpkin, and press normal Seedling management, planting after 2-4 days.
所述步骤(1)的育苗基质设置在育苗穴盘中。The seedling raising substrate in the step (1) is arranged in the seedling raising tray.
所述育苗穴盘的苗穴为正方形或圆形。The seedling hole of the seedling raising tray is square or circular.
所述育苗盘中的育苗基质为底部为3cm厚草炭土,上部为2cm厚蛭石,所述瓜类种子播种在所述草炭土和蛭石之间。The seedling raising substrate in the described seedling raising tray is peat soil with a thickness of 3 cm at the bottom and vermiculite with a thickness of 2 cm at the top, and the melon seeds are sown between the peat soil and the vermiculite.
所述瓜类为黄瓜、甜瓜或西瓜。The melons are cucumbers, melons or watermelons.
所述黄瓜或甜瓜的种子的播种密度为25cm×50cm均匀播种750粒。The sowing density of the seeds of the cucumber or muskmelon is 25cm×50cm and 750 seeds are evenly sown.
本发明将两种砧木的靠接及将瓜类接穗与砧木的插接同时进行,极大提高了操作效率,并大大降低了随之产生的管理成本。非常适用于嫁接育苗生产的工厂化。The present invention carries out the docking of two kinds of rootstocks and the plugging of melon scions and rootstocks at the same time, which greatly improves the operation efficiency and greatly reduces the management cost produced accordingly. It is very suitable for factory production of grafted seedlings.
采用本发明的方法,嫁接成活率达95%以上,由于双砧木的根系的作用,使幼苗长势明显好于单砧木的嫁接苗,并能更大程度减少黑籽南瓜对瓜类品质的影响。具有双砧木根系的瓜类作物如黄瓜在重茬严重地块,表现出很好的抗病性,田间发病株率明显低于单砧木嫁接的植株,产量提高30%以上,甜瓜和西瓜的果实糖度(可溶性固形物)与单砧木嫁接果实相同。By adopting the method of the present invention, the survival rate of grafting reaches more than 95%. Due to the effect of the root system of the double rootstock, the growth of the seedlings is obviously better than that of the grafted seedlings of the single rootstock, and the influence of the black-seeded pumpkin on the quality of melons can be reduced to a greater extent. Melon crops with double rootstock roots, such as cucumbers, show good disease resistance in heavily cropped fields, and the diseased plant rate in the field is significantly lower than that of single rootstock grafted plants, and the yield is increased by more than 30%. The fruit sugar content of melon and watermelon (Soluble solids) are the same as single rootstock grafted fruit.
具体实施方式Detailed ways
下面结合具体实施例对本发明作进一步的说明。The present invention will be further described below in conjunction with specific examples.
实施例1Example 1
一种瓜类双砧木嫁接方法,包括如下步骤:A kind of melon double stock grafting method, comprises the steps:
(1)将砧木白籽南瓜和黑籽南瓜相距1cm成对同时播种在装有育苗基质的苗穴为正方形的育苗穴盘中;(1) rootstock white-seed pumpkin and black-seed pumpkin are sown in pairs at the same time at a distance of 1 cm in the seedling hole that is equipped with a seedling-raising substrate in a square seedling-raising tray;
(2)将黄瓜种子在砧木播种6天时播种于装有育苗基质的育苗盘中,育苗盘中的育苗基质底部为3cm厚草炭土,黄瓜种子播种在草炭土上,再在上面覆盖2cm厚蛭石,黄瓜种子的播种密度为25cm×50cm均匀播种750粒;(2) Cucumber seeds were sowed in the seedling tray that the seedling substrate was housed when the rootstock was sown for 6 days. The bottom of the seedling substrate in the seedling tray was 3 cm thick peat soil, and the cucumber seeds were sown on the peat soil, and then covered with 2 cm thick leech Stone, the sowing density of cucumber seeds is 25cm×50cm sow 750 evenly;
(3)当砧木白籽南瓜第一真叶的直径达5cm时,将白籽南瓜去除第一真叶,黑籽南瓜去除生长点,利用靠接法将砧木白籽南瓜和黑籽南瓜嫁接在一起,用夹子固定;同时,将两片子叶展开角度为120度的瓜类幼苗在下胚轴离子叶2.0cm处,去除根部,将切下的黄瓜接穗用嫁接针斜插入白籽南瓜的下胚轴离子叶0.5cm位置处;(3) When the diameter of the first true leaf of rootstock white-seed pumpkin reaches 5cm, the first true leaf of white-seed pumpkin is removed, and the growth point of black-seed pumpkin is removed, and the rootstock white-seed pumpkin and black-seed pumpkin are grafted on Together, fix them with clips; at the same time, place two melon seedlings with cotyledons at an angle of 120 degrees at the hypocotyl ion leaf 2.0 cm, remove the root, and insert the cut cucumber scion obliquely into the hypogerm of white-seeded pumpkin with a grafting needle At the position of 0.5cm of the axis ion leaf;
(4)在25-30℃,用地膜直接覆盖在嫁接苗上以保证空气相对湿度为100%,同时用遮阳网覆盖避光,保持24小时,去除地膜,支起拱棚,改用塑料薄膜覆盖,使空气相对湿度为100%,继续用遮阳网覆盖避光48小时,去除遮阳网,按正常育苗管理12天,去除白籽南瓜第二真叶及生长点,按正常育苗管理,3天后定植。(4) At 25-30°C, cover the grafted seedlings directly with plastic film to ensure that the relative humidity of the air is 100%, and at the same time cover it with a sunshade net to avoid light, keep it for 24 hours, remove the plastic film, set up a shed, and cover with plastic film , so that the relative humidity of the air is 100%, continue to cover with sunshade nets to avoid light for 48 hours, remove the sunshade nets, and manage them according to normal seedling cultivation for 12 days, remove the second true leaf and growth point of white-seeded pumpkin, manage them according to normal seedling cultivation, and plant them after 3 days .
实施例2Example 2
一种瓜类双砧木嫁接方法,包括如下步骤:A kind of melon double stock grafting method, comprises the steps:
(1)将砧木白籽南瓜和黑籽南瓜相距1.5cm成对同时播种在装有育苗基质的苗穴为圆形的育苗穴盘中;(1) rootstock white-seed pumpkin and black-seed pumpkin are 1.5cm apart and sowed in pairs simultaneously in the seedling hole that seedling-raising substrate is housed in the circular seedling-raising tray;
(2)将甜瓜种子在砧木播种5天时播种于装有育苗基质的育苗盘中,育苗盘中的育苗基质底部为3cm厚草炭土,甜瓜种子播种在草炭土上,再在上面覆盖2cm厚蛭石,甜瓜种子的播种密度为25cm×50cm均匀播种750粒;(2) Melon seeds are sowed in the seedling tray that seedling substrate is housed when rootstock sows 5 days, and the bottom of the seedling substrate in the seedling tray is 3cm thick peat soil, and melon seed is sowed on peat soil, then covers 2cm thick leech above Stone, the sowing density of melon seeds is 25cm×50cm and 750 seeds are evenly sown;
(3)当砧木白籽南瓜第一真叶的直径达4.5cm时,将白籽南瓜去除第一真叶,黑籽南瓜去除生长点,利用靠接法将所述砧木白籽南瓜和黑籽南瓜嫁接在一起,用夹子固定;同时,将两片子叶展开角度为90度的瓜类幼苗在下胚轴离子叶1.5cm处,去除根部,将切下的甜瓜接穗用嫁接针斜插入白籽南瓜的下胚轴离子叶0.3cm位置处;(3) When the diameter of the first true leaf of the rootstock white-seed pumpkin reaches 4.5cm, the first true leaf of the white-seed pumpkin is removed, and the growth point of the black-seed pumpkin is removed, and the rootstock white-seed pumpkin and black-seed Graft pumpkins together and fix them with clips; at the same time, place two melon seedlings with cotyledons at an angle of 90 degrees at 1.5 cm from the hypocotyl ion leaf, remove the root, and insert the cut melon scion obliquely into the white-seeded pumpkin with a grafting needle The hypocotyl ion lobe 0.3cm position;
(4)在25-30℃,用地膜直接覆盖在嫁接苗上以保证空气相对湿度为100%,同时用遮阳网覆盖避光,保持22小时,去除地膜,支起拱棚,改用塑料薄膜覆盖,使空气相对湿度为100%,继续用遮阳网覆盖避光45小时,去除遮阳网,按正常育苗管理5天,去除白籽南瓜第二真叶及生长点,按正常育苗管理,2天后定植。(4) At 25-30°C, cover the grafted seedlings directly with plastic film to ensure that the relative humidity of the air is 100%, and at the same time cover it with a sunshade net to avoid light, keep it for 22 hours, remove the plastic film, set up an arch shed, and cover with plastic film , so that the relative air humidity is 100%, continue to cover with sunshade nets to avoid light for 45 hours, remove the sunshade nets, manage for 5 days according to normal seedling cultivation, remove the second true leaf and growth point of white-seeded pumpkin, manage according to normal seedling cultivation, and plant after 2 days .
实施例3Example 3
一种瓜类双砧木嫁接方法,包括如下步骤:A kind of melon double stock grafting method, comprises the steps:
(1)将砧木白籽南瓜和黑籽南瓜相距1.2cm成对同时播种在装有育苗基质的苗穴为正方形的育苗穴盘中;(1) rootstock white-seed pumpkin and black-seed pumpkin are 1.2cm apart and sowed in pairs simultaneously in the seedling hole that is equipped with seedling-raising matrix in the seedling-raising hole tray of square;
(2)将西瓜种子在砧木播种7天时播种于装有育苗基质的育苗盘中,育苗盘中的育苗基质底部为3cm厚草炭土,西瓜种子播种在草炭土上,再在上面覆盖2cm厚蛭石;(2) Watermelon seeds were sowed in the seedling tray that the seedling substrate was housed when the rootstock was sown for 7 days. The bottom of the seedling substrate in the seedling tray was 3cm thick peat soil, and the watermelon seeds were sown on the peat soil, and then covered with 2cm thick leeches stone;
(3)当砧木白籽南瓜第一真叶的直径达6cm时,将白籽南瓜去除第一真叶,黑籽南瓜去除生长点,利用靠接法将砧木白籽南瓜和黑籽南瓜嫁接在一起,用夹子固定;同时,将两片子叶展开角度为150度的瓜类幼苗在下胚轴离子叶2.5cm处,去除根部,将切下的瓜类接穗用嫁接针斜插入白籽南瓜的下胚轴离子叶0.7cm位置处;(3) When the diameter of the first true leaf of rootstock white-seed pumpkin reaches 6cm, the first true leaf of white-seed pumpkin is removed, and the growth point of black-seed pumpkin is removed, and the rootstock white-seed pumpkin and black-seed pumpkin are grafted on Together, fix them with clips; at the same time, put two melon seedlings whose cotyledons have an expansion angle of 150 degrees on the hypocotyl ion leaf 2.5cm, remove the root, and insert the cut melon scion obliquely into the lower part of the white-seeded pumpkin with a grafting needle. At the position of 0.7cm of hypocotyl ion lobe;
(4)在25-30℃,用地膜直接覆盖在嫁接苗上以保证空气相对湿度为100%,同时用遮阳网覆盖避光,保持26小时,去除地膜,支起拱棚,改用塑料薄膜覆盖,使空气相对湿度为100%,继续用遮阳网覆盖避光50小时,去除遮阳网,按正常育苗管理8天,去除白籽南瓜第二真叶及生长点,按正常育苗管理,4天后定植。(4) At 25-30°C, cover the grafted seedlings directly with mulch film to ensure that the relative air humidity is 100%, and at the same time cover it with a sunshade net to avoid light, keep it for 26 hours, remove the mulch film, set up an arch shed, and cover with plastic film , so that the relative humidity of the air is 100%, continue to cover with sunshade nets to avoid light for 50 hours, remove the sunshade nets, manage for 8 days according to normal seedling cultivation, remove the second true leaf and growth point of white-seeded pumpkin, manage according to normal seedling cultivation, and plant after 4 days .
实施例4Example 4
一种瓜类双砧木嫁接方法,包括如下步骤:A kind of melon double stock grafting method, comprises the steps:
(1)将砧木白籽南瓜和黑籽南瓜相距1.3cm成对同时播种在育苗基质中;(1) rootstock white-seed pumpkin and black-seed pumpkin are sowed in pairs simultaneously in the seedling-raising substrate at a distance of 1.3 cm;
(2)将黄瓜种子在砧木播种8天时播种于装有育苗基质的育苗盘中,育苗盘中的育苗基质底部为3cm厚草炭土,黄瓜种子播种在草炭土上,再在上面覆盖2cm厚蛭石,黄瓜种子的播种密度为25cm×50cm均匀播种750粒;(2) Cucumber seeds were sowed in the seedling tray that the seedling substrate was equipped with when the rootstock was sown for 8 days, the bottom of the seedling substrate in the seedling tray was 3cm thick peat soil, the cucumber seeds were sown on the peat soil, and covered with 2cm thick leeches Stone, the sowing density of cucumber seeds is 25cm×50cm sow 750 evenly;
(3)当砧木白籽南瓜第一真叶的直径达5.5cm时,将白籽南瓜去除第一真叶,黑籽南瓜去除生长点,利用靠接法将所述砧木白籽南瓜和黑籽南瓜嫁接在一起,用夹子固定;同时,将两片子叶展开角度为120度的瓜类幼苗在下胚轴离子叶2.5cm处,去除根部,将切下的黄瓜接穗用嫁接针斜插入白籽南瓜的下胚轴离子叶0.5cm位置处;(3) When the diameter of the first true leaf of the rootstock white-seed pumpkin reaches 5.5cm, the first true leaf of the white-seed pumpkin is removed, and the growth point of the black-seed pumpkin is removed, and the rootstock white-seed pumpkin and black-seed Graft the pumpkins together and fix them with clips; at the same time, place two melon seedlings with cotyledons at an angle of 120 degrees at 2.5 cm from the hypocotyl ion leaf, remove the root, and insert the cut cucumber scion obliquely into the white-seeded pumpkin with a grafting needle 0.5cm position of the hypocotyl ion lobe;
(4)在25-30℃,用地膜直接覆盖在嫁接苗上以保证空气相对湿度为100%,同时用遮阳网覆盖避光,保持24小时,去除地膜,支起拱棚,改用塑料薄膜覆盖,使空气相对湿度为100%,继续用遮阳网覆盖避光48小时,去除遮阳网,按正常育苗管理15天,去除白籽南瓜第二真叶及生长点,按正常育苗管理,3天后定植。(4) At 25-30°C, cover the grafted seedlings directly with plastic film to ensure that the relative humidity of the air is 100%, and at the same time cover it with a sunshade net to avoid light, keep it for 24 hours, remove the plastic film, set up a shed, and cover with plastic film , so that the relative humidity of the air is 100%, continue to cover with sunshade nets to avoid light for 48 hours, remove the sunshade nets, manage them according to normal seedling cultivation for 15 days, remove the second true leaves and growth points of white-seeded pumpkin, manage them according to normal seedling cultivation, and plant them after 3 days .
表1为用本发明的方法对黄瓜、甜瓜、西瓜进行嫁接实验数据。Table 1 is carrying out grafting experiment data to cucumber, muskmelon, watermelon with the method of the present invention.
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