CN101812489A - Culture medium for producing griseofulvin by fermentation - Google Patents
Culture medium for producing griseofulvin by fermentation Download PDFInfo
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- CN101812489A CN101812489A CN 201010175051 CN201010175051A CN101812489A CN 101812489 A CN101812489 A CN 101812489A CN 201010175051 CN201010175051 CN 201010175051 CN 201010175051 A CN201010175051 A CN 201010175051A CN 101812489 A CN101812489 A CN 101812489A
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- fermentation
- semen maydis
- maydis powder
- culture medium
- substratum
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- 238000000855 fermentation Methods 0.000 title claims abstract description 23
- 230000004151 fermentation Effects 0.000 title claims abstract description 23
- 239000001963 growth medium Substances 0.000 title claims abstract description 18
- DDUHZTYCFQRHIY-UHFFFAOYSA-N Negwer: 6874 Natural products COC1=CC(=O)CC(C)C11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 DDUHZTYCFQRHIY-UHFFFAOYSA-N 0.000 title claims description 20
- DDUHZTYCFQRHIY-RBHXEPJQSA-N griseofulvin Chemical compound COC1=CC(=O)C[C@@H](C)[C@@]11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 DDUHZTYCFQRHIY-RBHXEPJQSA-N 0.000 title claims description 20
- IIUZTXTZRGLYTI-UHFFFAOYSA-N Dihydrogriseofulvin Natural products COC1CC(=O)CC(C)C11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 IIUZTXTZRGLYTI-UHFFFAOYSA-N 0.000 title claims description 15
- UXWOXTQWVMFRSE-UHFFFAOYSA-N Griseoviridin Natural products O=C1OC(C)CC=C(C(NCC=CC=CC(O)CC(O)C2)=O)SCC1NC(=O)C1=COC2=N1 UXWOXTQWVMFRSE-UHFFFAOYSA-N 0.000 title claims description 15
- 229960002867 griseofulvin Drugs 0.000 title claims description 15
- 239000002609 medium Substances 0.000 claims abstract description 23
- 240000008042 Zea mays Species 0.000 claims abstract description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 15
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 15
- 235000005822 corn Nutrition 0.000 claims abstract description 15
- 238000011218 seed culture Methods 0.000 claims abstract description 13
- 235000013312 flour Nutrition 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims description 31
- 210000000582 semen Anatomy 0.000 claims description 30
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 20
- 239000002994 raw material Substances 0.000 claims description 19
- 238000002360 preparation method Methods 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 229910019142 PO4 Inorganic materials 0.000 claims description 10
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 10
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 10
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 10
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 10
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 10
- 239000011790 ferrous sulphate Substances 0.000 claims description 10
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 10
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 10
- 235000012204 lemonade/lime carbonate Nutrition 0.000 claims description 10
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 10
- 239000010452 phosphate Substances 0.000 claims description 10
- 229910052700 potassium Inorganic materials 0.000 claims description 10
- 239000011591 potassium Substances 0.000 claims description 10
- 229940001516 sodium nitrate Drugs 0.000 claims description 10
- 235000010344 sodium nitrate Nutrition 0.000 claims description 10
- 239000004317 sodium nitrate Substances 0.000 claims description 10
- 210000001161 mammalian embryo Anatomy 0.000 claims description 6
- 238000010298 pulverizing process Methods 0.000 claims description 6
- 239000004382 Amylase Substances 0.000 claims description 5
- 102000013142 Amylases Human genes 0.000 claims description 5
- 108010065511 Amylases Proteins 0.000 claims description 5
- 235000019418 amylase Nutrition 0.000 claims description 5
- 239000006260 foam Substances 0.000 claims description 5
- 206010055018 Gingival cyst Diseases 0.000 claims description 4
- 210000004907 gland Anatomy 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 8
- 235000009566 rice Nutrition 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052799 carbon Inorganic materials 0.000 abstract description 4
- 239000006052 feed supplement Substances 0.000 abstract 2
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 238000012262 fermentative production Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a culture medium for producing fermentation by fermentation, which comprises a seed culture medium and a fermentation medium. In the process of fermentation culture, when total sugar reduces to 2.0-3.0%, a feed supplement culture medium is added; and corn is used as a main carbon source to prepare the fermentation culture medium. Preferentially, the seed culture medium and the feed supplement culture medium are also prepared by using the corn as the main carbon source. Since the price of corn flour is much lower than that of rice flour, the production cost is greatly reduced; in addition, the corn flour has sufficient supply and stable quality, thereby ensuring the stability of fermentation production.
Description
Technical field
The invention belongs to the microbial fermentation engineering technical field, relate to a kind of substratum of producing griseofulvin by fermentation specifically.
Background technology
Grisovin (griseoflvin) belongs to the microbiotic of aromatic derivant class, is a kind of antifungal antibiotic, mainly treats the infection that Pi Fu Xian Pseudomonas causes, is used for the plant epiphyte resisting disease on agricultural.The generation bacterium that the fermentative production of domestic grisovin is used all be by China from the wild Penicillumpatulum4541 bacterial strain of row filter through too much obtaining for mutagenesis and directive breeding, the bacterial classification that the present invention uses also is the offspring of this bacterial strain.Since 1880s grisovin industrial fermentation is produced, the main carbon source of long-grained nonglutinous rice powder (or claiming rice meal) conduct of always using the southern long-grained nonglutinous rice that abounds with to be processed into.Rice is produced grisovin as main carbon source, and the source of goods is inadequate, raw materials quality there is not guarantee, and the production cost height.
Summary of the invention
The objective of the invention is to overcome the shortcoming of prior art, a kind of sufficient raw is provided, the substratum of the producing griseofulvin by fermentation of low production cost.
The object of the present invention is achieved like this: a kind of substratum of producing griseofulvin by fermentation comprises seed culture medium, fermention medium and supplemented medium; It is characterized in that: described fermention medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 13～17%, lime carbonate 0.5～0.7%, potassium primary phosphate 0.6～1%, sodium-chlor 0.3～0.5%, Repone K 0.5～0.7%, sal epsom 0.06～0.14%, ferrous sulfate 0.06～0.14%, ammonium sulfate 0.06～0.14%, SODIUMNITRATE 0.06～0.14%, surplus are water.
As a further improvement on the present invention, described seed culture medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 8～12%, lime carbonate 0.3～0.5%, potassium primary phosphate 0.3～0.5%, sodium-chlor 0.15～0.25%, Repone K 0.15～0.25%, sal epsom 0.06～0.14%, ferrous sulfate 0.06～0.14%, ammonium sulfate 0.06～0.14%, SODIUMNITRATE 0.06～0.14%, surplus are water.
Further improve as of the present invention, described supplemented medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 15～40%, amylase 0.02～0.1%, foam killer 0.05～0.3%, surplus are water.
Described Semen Maydis powder is to select yellow gingival cyst of mucous gland in the newborn corn for use, and perhaps flour corn forms through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
Described fermention medium, seed culture medium and supplemented medium, preparation method separately takes by weighing each raw material according to the above ratio, drops in the fermentor tank, stirs, and seals sterilization and makes.
The invention has the advantages that: the substratum of producing griseofulvin by fermentation of the present invention, use the abundant corn of produce to be processed into Semen Maydis powder as raw material, substitute long-term raw material with the rice meal producing griseofulvin by fermentation, produce grisovin by fermentation.Its advantage be following some: the Semen Maydis powder price is lower than rice meal, reduces the production cost of company greatly; The ample supply and prompt delivery of Semen Maydis powder, steady quality guarantees the stable of fermentative production; Purchasing department can purchase corn nearby, reduces purchase cost; Logistics department does not need large quantities of transportation by railroad rice, has alleviated transport pressure.
Embodiment
Embodiment 1: a kind of substratum of producing griseofulvin by fermentation, comprise seed culture medium, fermention medium and supplemented medium, described seed culture medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 10%, lime carbonate 0.4%, potassium primary phosphate 0.4%, sodium-chlor 0.2%, Repone K 0.2%, sal epsom 0.1%, ferrous sulfate 0.1%, ammonium sulfate 0.1%, SODIUMNITRATE 0.1%, surplus are water; Described fermention medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 15%, lime carbonate 0.6%, potassium primary phosphate 0.8%, sodium-chlor 0.4%, Repone K 0.6%, sal epsom 0.1%, ferrous sulfate 0.1%, ammonium sulfate 0.1%, SODIUMNITRATE 0.1%, surplus are water; Described supplemented medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 28%, amylase 0.05%, foam killer 0.15%, surplus are water.Wherein, described Semen Maydis powder is to select for use yellow gingival cyst of mucous gland in the newborn corn to form through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
Embodiment 2: a kind of substratum of producing griseofulvin by fermentation, comprise seed culture medium, fermention medium and supplemented medium, described seed culture medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 9%, lime carbonate 0.35%, potassium primary phosphate 0.35%, sodium-chlor 0.16%, Repone K 0.17%, sal epsom 0.08%, ferrous sulfate 0.07%, ammonium sulfate 0.09%, SODIUMNITRATE 0.08%, surplus are water.Described fermention medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 13%, lime carbonate 0.55%, potassium primary phosphate 0.7%, sodium-chlor 0.35%, Repone K 0.56%, sal epsom 0.08%, ferrous sulfate 0.09%, ammonium sulfate 0.07%, SODIUMNITRATE 0.08%, surplus are water; Described supplemented medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 25%, amylase 0.03%, foam killer 0.1%, surplus are water.Wherein, described Semen Maydis powder is to select for use flour corn to form through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
Embodiment 3: a kind of substratum of producing griseofulvin by fermentation, comprise seed culture medium, fermention medium and supplemented medium, described seed culture medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 11%, lime carbonate 0.42%, potassium primary phosphate 0.45%, sodium-chlor 0.22%, Repone K 0.21%, sal epsom 0.12%, ferrous sulfate 0.13%, ammonium sulfate 0.11%, SODIUMNITRATE 0.12%, surplus are water; Described fermention medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 17%, lime carbonate 0.65%, potassium primary phosphate 0.9%, sodium-chlor 0.45%, Repone K 0.65%, sal epsom 0.11%, ferrous sulfate 0.12%, ammonium sulfate 0.10%, SODIUMNITRATE 0.11%, surplus are water; Described supplemented medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 30%, amylase 0.08%, foam killer 0.2%, surplus are water.Wherein, described Semen Maydis powder is to select for use flour corn to form through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
Claims (5)
1. the substratum of a producing griseofulvin by fermentation comprises seed culture medium, fermention medium and supplemented medium; It is characterized in that: described fermention medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 13～17%, lime carbonate 0.5～0.7%, potassium primary phosphate 0.6～1%, sodium-chlor 0.3～0.5%, Repone K 0.5～0.7%, sal epsom 0.06～0.14%, ferrous sulfate 0.06～0.14%, ammonium sulfate 0.06～0.14%, SODIUMNITRATE 0.06～0.14%, surplus are water.
2. according to the substratum of the described producing griseofulvin by fermentation of claim 1, it is characterized in that: described seed culture medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 8～12%, lime carbonate 0.3～0.5%, potassium primary phosphate 0.3～0.5%, sodium-chlor 0.15～0.25%, Repone K 0.15～0.25%, sal epsom 0.06～0.14%, ferrous sulfate 0.06～0.14%, ammonium sulfate 0.06～0.14%, SODIUMNITRATE 0.06～0.14%, surplus are water.
3. according to the substratum of claim 1 or 2 described producing griseofulvin by fermentation, it is characterized in that: described supplemented medium is formed by the preparation of raw material of following weight ratio, and Semen Maydis powder 15～40%, amylase 0.02～0.1%, foam killer 0.05～0.3%, surplus are water.
4. according to the substratum of claim 1 or 2 described producing griseofulvin by fermentation, it is characterized in that: described Semen Maydis powder is to select yellow gingival cyst of mucous gland in the newborn corn for use, and perhaps flour corn forms through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
5. according to the substratum of the described producing griseofulvin by fermentation of claim 3, it is characterized in that: described Semen Maydis powder is to select yellow gingival cyst of mucous gland in the newborn corn for use, and perhaps flour corn forms through the embryo of peeling, take off, pulverizing; Fineness 80 orders of Semen Maydis powder sieve more than 60%, and sugared content is more than 80%, and protein content is less than 9%, and moisture is less than 9%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010175051 CN101812489A (en) | 2010-04-29 | 2010-04-29 | Culture medium for producing griseofulvin by fermentation |
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| CN 201010175051 CN101812489A (en) | 2010-04-29 | 2010-04-29 | Culture medium for producing griseofulvin by fermentation |
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| CN101812489A true CN101812489A (en) | 2010-08-25 |
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| CN 201010175051 Pending CN101812489A (en) | 2010-04-29 | 2010-04-29 | Culture medium for producing griseofulvin by fermentation |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103275047A (en) * | 2013-05-23 | 2013-09-04 | 内蒙古格林特制药有限责任公司 | Preparation method of griseofulvin |
| CN106480121A (en) * | 2016-09-27 | 2017-03-08 | 宁夏泰瑞制药股份有限公司 | A kind of culture medium of utilization penicillium patulum producing griseofulvin by fermentation and cultural method |
| CN108641969A (en) * | 2018-06-21 | 2018-10-12 | 赤峰制药股份有限公司 | A kind of griseofulvin production bacterium culture medium |
| CN110317841A (en) * | 2019-07-10 | 2019-10-11 | 内蒙古格林特制药有限责任公司 | A kind of griseofulvin fermentation production medium |
| CN110669678A (en) * | 2019-10-22 | 2020-01-10 | 赤峰制药股份有限公司 | Griseofulvin strain solid culture medium |
| CN112094874A (en) * | 2020-09-16 | 2020-12-18 | 内蒙古格林特制药有限责任公司 | Culture medium for producing griseofulvin through fermentation |
| CN115644062A (en) * | 2022-11-03 | 2023-01-31 | 天津科润农业科技股份有限公司 | Culture medium and method for improving embryo induction rate of Chinese cabbage with embryos difficult to produce |
-
2010
- 2010-04-29 CN CN 201010175051 patent/CN101812489A/en active Pending
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| 《亚热带植物通讯》 19941231 沈玉芬 等 灰黄霉素产生菌耐前体变株F-1012的选育及其发酵特性 19-25 1-5 第23卷, 第2期 2 * |
| 《药物生物技术》 19951231 林琳 等 灰黄霉素产生菌耐前体变株F-208的选育及其特性的研究 28-32 1-4 第2卷, 第4期 2 * |
| 《西南农业学报》 20051231 杨红玉 等 灰霉菌培养及其对拟南芥的侵染 431-434 1-5 第18卷, 第4期 2 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103275047A (en) * | 2013-05-23 | 2013-09-04 | 内蒙古格林特制药有限责任公司 | Preparation method of griseofulvin |
| CN103275047B (en) * | 2013-05-23 | 2014-09-03 | 内蒙古格林特制药有限责任公司 | Preparation method of griseofulvin |
| CN106480121A (en) * | 2016-09-27 | 2017-03-08 | 宁夏泰瑞制药股份有限公司 | A kind of culture medium of utilization penicillium patulum producing griseofulvin by fermentation and cultural method |
| CN108641969A (en) * | 2018-06-21 | 2018-10-12 | 赤峰制药股份有限公司 | A kind of griseofulvin production bacterium culture medium |
| CN110317841A (en) * | 2019-07-10 | 2019-10-11 | 内蒙古格林特制药有限责任公司 | A kind of griseofulvin fermentation production medium |
| CN110317841B (en) * | 2019-07-10 | 2022-11-22 | 内蒙古格林特制药有限责任公司 | Fermentation production culture medium for griseofulvin |
| CN110669678A (en) * | 2019-10-22 | 2020-01-10 | 赤峰制药股份有限公司 | Griseofulvin strain solid culture medium |
| CN112094874A (en) * | 2020-09-16 | 2020-12-18 | 内蒙古格林特制药有限责任公司 | Culture medium for producing griseofulvin through fermentation |
| CN115644062A (en) * | 2022-11-03 | 2023-01-31 | 天津科润农业科技股份有限公司 | Culture medium and method for improving embryo induction rate of Chinese cabbage with embryos difficult to produce |
| CN115644062B (en) * | 2022-11-03 | 2023-11-17 | 天津科润农业科技股份有限公司 | Culture medium and method for improving induction rate of embryo of difficult-to-embryo Chinese cabbage |
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Application publication date: 20100825 |