CN101703561A - Eucommia bark effective position for treating osteoporosis and preparation method thereof - Google Patents
Eucommia bark effective position for treating osteoporosis and preparation method thereof Download PDFInfo
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- CN101703561A CN101703561A CN200910186208A CN200910186208A CN101703561A CN 101703561 A CN101703561 A CN 101703561A CN 200910186208 A CN200910186208 A CN 200910186208A CN 200910186208 A CN200910186208 A CN 200910186208A CN 101703561 A CN101703561 A CN 101703561A
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- eucommia bark
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- 208000001132 Osteoporosis Diseases 0.000 title claims abstract description 28
- 241000208688 Eucommia Species 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 34
- 238000010828 elution Methods 0.000 claims abstract description 16
- 239000011347 resin Substances 0.000 claims abstract description 14
- 229920005989 resin Polymers 0.000 claims abstract description 14
- 230000001476 alcoholic effect Effects 0.000 claims description 37
- 239000000284 extract Substances 0.000 claims description 36
- 239000003480 eluent Substances 0.000 claims description 27
- 241000208689 Eucommia ulmoides Species 0.000 claims description 19
- 238000001035 drying Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000012141 concentrate Substances 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 10
- 239000000796 flavoring agent Substances 0.000 claims description 8
- 235000019634 flavors Nutrition 0.000 claims description 8
- 239000006071 cream Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- 208000024891 symptom Diseases 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 5
- 239000012567 medical material Substances 0.000 claims description 4
- 230000000274 adsorptive effect Effects 0.000 claims 2
- 238000000638 solvent extraction Methods 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 abstract 4
- 239000002674 ointment Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 10
- 201000010099 disease Diseases 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 210000000988 bone and bone Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000001179 sorption measurement Methods 0.000 description 5
- 229940079593 drug Drugs 0.000 description 4
- 230000000857 drug effect Effects 0.000 description 4
- 239000000469 ethanolic extract Substances 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- 238000010992 reflux Methods 0.000 description 4
- 241000700159 Rattus Species 0.000 description 3
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 238000002481 ethanol extraction Methods 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 229930002330 retinoic acid Natural products 0.000 description 3
- 229960001727 tretinoin Drugs 0.000 description 3
- 230000037182 bone density Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 208000008035 Back Pain Diseases 0.000 description 1
- 206010017076 Fracture Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 208000008930 Low Back Pain Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000002805 bone matrix Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000001009 osteoporotic effect Effects 0.000 description 1
- 238000011552 rat model Methods 0.000 description 1
- 108010048734 sclerotin Proteins 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to an eucommia bark effective position for treating osteoporosis and a preparation method thereof, which are characterized in that eucommia bark is extracted by using 0 to 90 percent ethanol solution, and extraction liquid is concentrated to have no ethanol taste; the concentrated extraction liquid passes through a nonpolar macroporous resin column and is eluted by using 0 and 30 percent ethanol solution, and elution liquid is collected and merged; and the collected and merged elution liquid is concentrated and dried to prepare black brown dried ointment which is the eucommia bark effective position for treating the osteoporosis. The eucommia bark effective position has the advantages that: compared with other positions, the obtained effective position can treat the osteoporosis more effectively.
Description
Technical field
The present invention relates to a kind of medicine for the treatment of osteoporosis, is the medicine of feedstock production with the Cortex Eucommiae specifically, the invention still further relates to the preparation method of this medicine, belongs to the field of Chinese medicines.
Background technology
Osteoporosis is impaired with the osseous tissue microstructure, bone ore deposit composition and bone matrix equal proportion ground constantly reduce, the sclerotin attenuation, bone trabecula quantity reduces, the disease of a kind of general metabolism obstacle that increase of bone fragility and risk of fractures degree raise, be a kind of commonly encountered diseases of postmenopausal women and old people, become one of human modal disease.China's traditional Chinese medical science thinks that osteoporotic basic reason is " suffering from a deficiency of the kidney ".The Cortex Eucommiae is the Chinese medicine of China, and its property is sweet, and temperature is returned liver, kidney channel, has invigorating the liver and kidney, bone and muscle strengthening, and antiabortive effect clinically is used for diseases such as hypertension, lumbago due to renal deficiency, weakness of the waist and knees, osteoporosis.
Because the basic research of the Cortex Eucommiae is deep not enough, the Cortex Eucommiae is clear and definite not enough to the effective site of various disease, a kind of Cortex Eucommiae single medicinal material system " full eucommia bark capsules " is arranged in the market, its method for making is according to the 12 in the Sanitation Ministry medicine standard Chinese traditional patent formulation preparation, all effective ingredient of the Cortex Eucommiae are all extracted, promptly blood pressure lowering can be used for, also invigorating the liver and kidney, bone and muscle strengthening can be used for.But different diseases has its different effective substance, all extracts active ingredients is come out to be used for the treatment of a kind of disease make utilization ratio of drug low, and drug waste is big.
Summary of the invention
The objective of the invention is to provides a kind of eucommia bark effective position for the treatment of osteoporosis at top described deficiency, makes it have best drug effect active to treatment osteoporosis symptom.
Another object of the present invention is to provide a kind of preparation method for the treatment of the eucommia bark effective position of osteoporosis.
The technical solution adopted in the present invention is:
A kind of eucommia bark effective position for the treatment of osteoporosis is characterized in that: it is to be made by following steps,
(1) extract: with concentration is that 0～80% alcoholic solution extracts Cortex Eucommiae medical material or decoction pieces 1～5 time, extracts 1-3 hour with 5-20 times of weight solvent at every turn, and extracting solution merges and is concentrated into that not have the alcohol flavor and make its relative density be 1.05～1.25, gets eucommia ulmoides extract.
(2) separate: make into suspension adding entry after the cooling of step (1) gained eucommia ulmoides extract, last nonpolar macroporous adsorption resin (as D101, GDX-101～105, GDX-201～203, GDX-301, HP-10, HP-20 etc.) post, elder generation's water eluting, reuse concentration is the alcoholic solution eluting below 30%, comprise that concentration is 30% alcoholic solution, merges eluent then.
(3) drying: step (2) gained eluent concentrate drying is become the dried cream of pitchy promptly.
In the described step (2), type of elution can be directly is alcoholic solution eluting below 30% with concentration, comprises that concentration is 30% alcoholic solution, collects eluent.
Drying means in the described step (3) also can be: step (2) gained eluent directly is spray dried to powder behind concentrating under reduced pressure.
Drying means in the described step (3) also can be: step (2) gained eluent concentrating under reduced pressure is become behind the thick paste shape lyophilization more promptly.
In the described step (3), concentrate control temperature≤60 ℃.
A kind of preparation method for the treatment of the eucommia bark effective position of osteoporosis is characterized in that: it is to be made by following steps,
(1) extract: with concentration is that 0～80% alcoholic solution extracts Cortex Eucommiae medical material or decoction pieces 1～5 time, extracts 1-3 hour with 5-20 times of weight alcoholic solution at every turn, and extracting solution merges and is concentrated into that not have the alcohol flavor and make its relative density be 1.05～1.25, gets eucommia ulmoides extract.
(2) separate: make into suspension adding entry after the cooling of step (1) gained eucommia ulmoides extract, last nonpolar macroporous adsorption resin (as D101, GDX-101～105, GDX-201～203, GDX-301, HP-10, HP-20 etc.) post, elder generation's water eluting, reuse concentration is the alcoholic solution eluting below 30%, comprise that concentration is 30% alcoholic solution, merges eluent then.
(3) drying: step (2) gained eluent concentrate drying is become the dried cream of pitchy promptly.
In the described step (2), type of elution can be directly is alcoholic solution eluting below 30% with concentration, comprises that concentration is 30% alcoholic solution, collects eluent.
Drying means in the described step (3) also can be: step (2) gained eluent directly is spray dried to powder behind concentrating under reduced pressure.
Drying means in the described step (3) also can be: step (2) gained eluent concentrating under reduced pressure is become behind the thick paste shape lyophilization more promptly.
In the described step (3), concentrate control temperature≤60 ℃.
Beneficial effect of the present invention: the present invention separates Cortex Eucommiae water-alcohol extraction through nonpolar macroporous adsorption resin, reuse concentration is that 0～30% ethanol carries out eluting, the effective site of the osteoporosis that obtains medical treatment, make curative effect of medication good, instant effect, other effective site of the Cortex Eucommiae of separating can be used for the treatment of other disease, has improved utilization ratio of drug.
The specific embodiment
Embodiment one:
Get Cortex Eucommiae decoction pieces 5kg, with concentration is 80% alcoholic solution reflux, extract, three times, alcoholic solution with 8 times of weight extracted 2 hours, collect ethanol extract, be concentrated into the eucommia ulmoides extract that does not have alcohol flavor (relative density 1.2), make into suspension with adding entry after the eucommia ulmoides extract cooling, last non-polar macroporous resin (D101) post, successively water, concentration are that 30% alcoholic solution is washed till eluent and is colourless respectively, water and 30% ethanol elution are merged, be that concentrate drying becomes the dried cream of pitchy promptly under 60 ℃ the situation in temperature.
Embodiment two:
Get Cortex Eucommiae decoction pieces 5kg, with concentration is 40% alcoholic solution reflux, extract, three times, at every turn with the ethanol extraction of 8 times of weight 2 hours, collect and merge ethanol extract, be concentrated into the eucommia ulmoides extract that does not have alcohol flavor (relative density 1.1), make into suspension with adding entry after the eucommia ulmoides extract cooling, last non-polar macroporous resin (GDX-201) post, successively water, concentration are that 30% alcoholic solution is washed till eluent and is colourless respectively, water and 30% ethanol elution are merged, be under 40 ℃ the situation, directly to be spray dried to powder behind the concentrating under reduced pressure in temperature.
Embodiment three:
Get Cortex Eucommiae decoction pieces 5kg, with water boiling and extraction three times, at every turn with the water extraction of 8 times of weight 2 hours, collect and merge aqueous extract, be concentrated into relative density and be 1.05 eucommia ulmoides extract, eucommia ulmoides extract cooling back is added low amounts of water make into suspension, last non-polar macroporous resin (HP-20) post, successively water, concentration are that 30% alcoholic solution is washed till eluent and is colourless respectively, water and 30% ethanol elution are merged, be under 40 ℃ the situation in temperature, concentrating under reduced pressure becomes behind the thick paste shape lyophilization more promptly.
Embodiment four:
Get Cortex Eucommiae decoction pieces 5kg, with concentration is 40% alcoholic solution reflux, extract, five times, at every turn with the ethanol extraction of 5 times of weight 1 hour, collect the merging ethanol extract, be concentrated into the eucommia ulmoides extract that does not have alcohol flavor (relative density 1.05), make into suspension adding entry after the eucommia ulmoides extract cooling, last non-polar macroporous resin (GDX-301) post, with concentration is that 30% alcoholic solution is washed till eluent and is colourless, with the gained eluent in temperature is under 20 ℃ the situation, directly to be spray dried to powder behind the concentrating under reduced pressure.
Embodiment five:
Get Cortex Eucommiae decoction pieces 5kg, with concentration be 50% alcoholic solution reflux, extract, once, at every turn with the ethanol extraction of 20 times of weight 3 hours, collect ethanol extract, be concentrated into the eucommia ulmoides extract that does not have alcohol flavor (relative density 1.25), make into suspension adding entry after the eucommia ulmoides extract cooling, last non-polar macroporous resin (GDX-203) post, with concentration is that 20% alcoholic solution is washed till eluent and is colourless, is that concentrate drying becomes the dried cream of pitchy promptly under 50 ℃ the situation in temperature with the gained eluent.
The present invention utilizes that the osteoporosis rat model is object of study due to the retinoic acid, respectively Cortex Eucommiae water-alcohol extraction is carried out drug effect relatively through the isolating different parts of nonpolar macroporous adsorption resin, the result shows, concentration is that 0～30% alcoholic solution eluate shows best drug effect.
For superiority of the present invention, the present invention has designed two comparative experimentss:
1. get and add entry after the eucommia ulmoides extract cooling of gained of embodiment 2 and make into suspension, last non-polar macroporous resin (GDX-201) post, successively water, concentration are that 30% alcoholic solution, concentration are that 50% alcoholic solution, concentration are that 70% alcoholic solution and concentration are 90% alcoholic solution eluting respectively, collect the eluting position that obtains respectively, utilize retinoic acid to make the female rats osteoporosis model, with the positive medicine of ALPHA D3, by observing rat bone density drug effect has been carried out relatively in each position, the results are shown in Table 1.Cortex Eucommiae extract is after nonpolar macroporous adsorption resin separates as can be seen from Table 1, and water elution position activity is the strongest, secondly is 30% ethanol elution position.50%～90% ethanol elution position activity is not strong.According to this result, think that water elution position and 30% ethanol elution position are Cortex Eucommiae osteoporosis effective site.
Table 1 Cortex Eucommiae different parts to the influence of osteoporosis rat bone density due to the retinoic acid (min ± s, n=10)
Compare with model group: * P＜0.05, * * P＜0.01; Compare with ALPHA D3: △ P＜0.05
2. water and the 30% ethanol elution merging back extractum interest rate to embodiment 1～3 gained compares, and the results are shown in Table 2.As can be seen from Table 2, be used to the different yield that can influence effective site of the concentration of alcohol that extracts.Concentration of alcohol is low more, and the interest rate of effective site is high more.
The water of table 2 embodiment 1～3 gained and 30% ethanol elution merge back extractum interest rate
Claims (10)
1. eucommia bark effective position for the treatment of osteoporosis, it is characterized in that: it is to be made by following steps,
(1) extract: with concentration is that 0～80% alcoholic solution extracts Cortex Eucommiae medical material or decoction pieces 1～5 time, and at every turn with solvent extraction 1-3 hour of 5-20 times of weight, extracting solution merges and is concentrated into that not have the alcohol flavor and make its relative density be 1.05～1.25, gets eucommia ulmoides extract;
(2) separate: make into suspension adding entry after the cooling of step (1) gained eucommia ulmoides extract, last nonpolarity macroporous adsorptive resins chromatographic column, first water eluting, reuse concentration is the alcoholic solution eluting below 30%, comprise that concentration is 30% alcoholic solution, merges eluent then; (3) drying: step (2) gained eluent concentrate drying is become the dried cream of pitchy promptly.
2. a kind of eucommia bark effective position for the treatment of osteoporosis according to claim 1, it is characterized in that: in the described step (2), type of elution can be directly is alcoholic solution eluting below 30% with concentration, comprises that concentration is 30% alcoholic solution, collects eluent.
3. a kind of eucommia bark effective position for the treatment of osteoporosis according to claim 1 is characterized in that: right wants the drying means in the described step (3) to be: step (2) gained eluent directly is spray dried to powder behind concentrating under reduced pressure.
4. a kind of eucommia bark effective position for the treatment of osteoporosis according to claim 1 is characterized in that: the drying means in the described step (3) is: step (2) gained eluent concentrating under reduced pressure is become behind the thick paste shape lyophilization more promptly.
5. according to claim 1,3 or 4 described a kind of eucommia bark effective positions for the treatment of osteoporosis, it is characterized in that: in the described step (3), concentrate control temperature≤60 ℃.
6. preparation method for the treatment of the eucommia bark effective position of osteoporosis symptom, it is characterized in that: it is to be made by following steps,
(1) extract: with concentration is that 0～80% alcoholic solution extracts Cortex Eucommiae medical material or decoction pieces 1～5 time, and the alcoholic solution with 5-20 times of weight extracted 1-3 hour at every turn, and extracting solution merges and is concentrated into that not have the alcohol flavor and make its relative density be 1.05～1.25, gets eucommia ulmoides extract;
(2) separate: make into suspension adding entry after the cooling of step (1) gained eucommia ulmoides extract, last nonpolarity macroporous adsorptive resins chromatographic column, first water eluting, reuse concentration is the alcoholic solution eluting below 30%, comprise that concentration is 30% alcoholic solution, merges eluent then;
(3) drying: step (2) gained eluent concentrate drying is become the dried cream of pitchy promptly.
7. a kind of preparation method for the treatment of the eucommia bark effective position of osteoporosis symptom according to claim 6, it is characterized in that: in the described step (2), type of elution can be directly is alcoholic solution eluting below 30% with concentration, comprises that concentration is 30% alcoholic solution, collects eluent.
8. a kind of preparation method for the treatment of the eucommia bark effective position of osteoporosis symptom according to claim 6 is characterized in that: the drying means in the described step (3) also can be: step (2) gained eluent directly is spray dried to powder behind concentrating under reduced pressure.
9. a kind of preparation method for the treatment of the eucommia bark effective position of osteoporosis symptom according to claim 6 is characterized in that: the drying means in the described step (3) also can be: step (2) gained eluent concentrating under reduced pressure is become behind the thick paste shape lyophilization more promptly.
10. according to claim 6,8 or 9 described a kind of preparation methoies for the treatment of the eucommia bark effective position of osteoporosis symptom, it is characterized in that: in the described step (3), concentrate control temperature≤60 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910186208A CN101703561A (en) | 2009-10-13 | 2009-10-13 | Eucommia bark effective position for treating osteoporosis and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910186208A CN101703561A (en) | 2009-10-13 | 2009-10-13 | Eucommia bark effective position for treating osteoporosis and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101912433A (en) * | 2010-08-30 | 2010-12-15 | 南通远大生物科技发展有限公司 | Method for extracting active component for resisting osteoporosis from eucommia bark |
| CN111686147A (en) * | 2020-06-28 | 2020-09-22 | 江西普正制药股份有限公司 | Eucommia ulmoides extract and application thereof in treating osteoporosis |
| CN113413395A (en) * | 2021-06-30 | 2021-09-21 | 江西普正制药股份有限公司 | Composition containing pinoresinol diglucoside and preparation method and application thereof |
| CN115844941A (en) * | 2022-12-26 | 2023-03-28 | 江西普正制药股份有限公司 | Composition of eucommia ulmoides extract and application of composition in preparation of anti-osteoporosis medicine |
-
2009
- 2009-10-13 CN CN200910186208A patent/CN101703561A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101912433A (en) * | 2010-08-30 | 2010-12-15 | 南通远大生物科技发展有限公司 | Method for extracting active component for resisting osteoporosis from eucommia bark |
| CN111686147A (en) * | 2020-06-28 | 2020-09-22 | 江西普正制药股份有限公司 | Eucommia ulmoides extract and application thereof in treating osteoporosis |
| CN113413395A (en) * | 2021-06-30 | 2021-09-21 | 江西普正制药股份有限公司 | Composition containing pinoresinol diglucoside and preparation method and application thereof |
| CN115844941A (en) * | 2022-12-26 | 2023-03-28 | 江西普正制药股份有限公司 | Composition of eucommia ulmoides extract and application of composition in preparation of anti-osteoporosis medicine |
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Open date: 20100512 |