CN101406203B - Plant growth regulator and production thereof - Google Patents
Plant growth regulator and production thereof Download PDFInfo
- Publication number
- CN101406203B CN101406203B CN2007101641597A CN200710164159A CN101406203B CN 101406203 B CN101406203 B CN 101406203B CN 2007101641597 A CN2007101641597 A CN 2007101641597A CN 200710164159 A CN200710164159 A CN 200710164159A CN 101406203 B CN101406203 B CN 101406203B
- Authority
- CN
- China
- Prior art keywords
- parts
- earthworm
- supernatant
- compound
- add
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 36
- 239000005648 plant growth regulator Substances 0.000 title claims abstract description 20
- 241000361919 Metaphire sieboldi Species 0.000 claims abstract description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 26
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 26
- 102000019197 Superoxide Dismutase Human genes 0.000 claims abstract description 24
- 108010012715 Superoxide dismutase Proteins 0.000 claims abstract description 24
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 22
- 241000223253 Rhodotorula glutinis Species 0.000 claims abstract description 18
- 241001474374 Blennius Species 0.000 claims abstract description 17
- 230000000975 bioactive effect Effects 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 17
- 229910052761 rare earth metal Inorganic materials 0.000 claims abstract description 14
- 150000002910 rare earth metals Chemical class 0.000 claims abstract description 14
- 229940024606 amino acid Drugs 0.000 claims abstract description 13
- 150000001413 amino acids Chemical class 0.000 claims abstract description 13
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 11
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims abstract description 11
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000011702 manganese sulphate Substances 0.000 claims abstract description 11
- 235000007079 manganese sulphate Nutrition 0.000 claims abstract description 11
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims abstract description 11
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 claims abstract description 11
- 235000012239 silicon dioxide Nutrition 0.000 claims abstract description 11
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 11
- 229940046009 vitamin E Drugs 0.000 claims abstract description 11
- 239000011709 vitamin E Substances 0.000 claims abstract description 11
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 11
- 102000030523 Catechol oxidase Human genes 0.000 claims abstract description 9
- 108010031396 Catechol oxidase Proteins 0.000 claims abstract description 9
- 235000000346 sugar Nutrition 0.000 claims abstract description 8
- 150000001875 compounds Chemical class 0.000 claims description 50
- 239000007788 liquid Substances 0.000 claims description 49
- 239000006228 supernatant Substances 0.000 claims description 46
- 201000010099 disease Diseases 0.000 claims description 32
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 32
- 240000008042 Zea mays Species 0.000 claims description 30
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 30
- 239000008363 phosphate buffer Substances 0.000 claims description 29
- 239000003795 chemical substances by application Substances 0.000 claims description 28
- 230000003750 conditioning effect Effects 0.000 claims description 28
- 239000012530 fluid Substances 0.000 claims description 26
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 24
- 235000005822 corn Nutrition 0.000 claims description 24
- 238000005119 centrifugation Methods 0.000 claims description 22
- 102000004118 Ammonia-Lyases Human genes 0.000 claims description 19
- 108090000673 Ammonia-Lyases Proteins 0.000 claims description 19
- 238000012545 processing Methods 0.000 claims description 19
- 239000007787 solid Substances 0.000 claims description 18
- 238000000605 extraction Methods 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 17
- 239000013543 active substance Substances 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 16
- 229920003266 Leaf® Polymers 0.000 claims description 14
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 14
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 14
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 14
- MVZXTUSAYBWAAM-UHFFFAOYSA-N iron;sulfuric acid Chemical compound [Fe].OS(O)(=O)=O MVZXTUSAYBWAAM-UHFFFAOYSA-N 0.000 claims description 12
- 239000011259 mixed solution Substances 0.000 claims description 12
- 239000013049 sediment Substances 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 11
- -1 PPO compound Chemical class 0.000 claims description 10
- 239000007853 buffer solution Substances 0.000 claims description 10
- 229910000358 iron sulfate Inorganic materials 0.000 claims description 10
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 10
- 239000011686 zinc sulphate Substances 0.000 claims description 10
- 235000009529 zinc sulphate Nutrition 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 9
- 239000006166 lysate Substances 0.000 claims description 9
- 239000002504 physiological saline solution Substances 0.000 claims description 9
- 150000003839 salts Chemical class 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 8
- 241000251468 Actinopterygii Species 0.000 claims description 8
- 241001093951 Ailanthus altissima Species 0.000 claims description 8
- 240000002234 Allium sativum Species 0.000 claims description 8
- 235000007650 Aralia spinosa Nutrition 0.000 claims description 8
- 235000003092 Artemisia dracunculus Nutrition 0.000 claims description 8
- 240000001851 Artemisia dracunculus Species 0.000 claims description 8
- 235000011201 Ginkgo Nutrition 0.000 claims description 8
- 244000194101 Ginkgo biloba Species 0.000 claims description 8
- 235000008100 Ginkgo biloba Nutrition 0.000 claims description 8
- 240000007049 Juglans regia Species 0.000 claims description 8
- 235000009496 Juglans regia Nutrition 0.000 claims description 8
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims description 8
- 244000302512 Momordica charantia Species 0.000 claims description 8
- 235000009811 Momordica charantia Nutrition 0.000 claims description 8
- 235000009812 Momordica cochinchinensis Nutrition 0.000 claims description 8
- 235000018365 Momordica dioica Nutrition 0.000 claims description 8
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 8
- 240000000528 Ricinus communis Species 0.000 claims description 8
- 235000004443 Ricinus communis Nutrition 0.000 claims description 8
- 241000612118 Samolus valerandi Species 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 241000949456 Zanthoxylum Species 0.000 claims description 8
- 244000273928 Zingiber officinale Species 0.000 claims description 8
- 235000006886 Zingiber officinale Nutrition 0.000 claims description 8
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 8
- 235000004611 garlic Nutrition 0.000 claims description 8
- 235000008397 ginger Nutrition 0.000 claims description 8
- 210000002966 serum Anatomy 0.000 claims description 8
- 235000014347 soups Nutrition 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 235000020234 walnut Nutrition 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 6
- 101001091385 Homo sapiens Kallikrein-6 Proteins 0.000 claims description 6
- 102100034866 Kallikrein-6 Human genes 0.000 claims description 6
- 239000012267 brine Substances 0.000 claims description 6
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 6
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims description 6
- 238000000108 ultra-filtration Methods 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 4
- 238000000855 fermentation Methods 0.000 claims description 4
- 230000004151 fermentation Effects 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 238000009413 insulation Methods 0.000 claims description 4
- 229960004441 tyrosine Drugs 0.000 claims description 4
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 3
- 229930003268 Vitamin C Natural products 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 239000013530 defoamer Substances 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 241001233061 earthworms Species 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000008101 lactose Substances 0.000 claims description 3
- 230000003204 osmotic effect Effects 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000002002 slurry Substances 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 claims description 3
- 235000019154 vitamin C Nutrition 0.000 claims description 3
- 239000011718 vitamin C Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 2
- 235000015393 sodium molybdate Nutrition 0.000 claims 4
- 239000011684 sodium molybdate Substances 0.000 claims 4
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims 4
- 239000000126 substance Substances 0.000 abstract description 6
- 108700023158 Phenylalanine ammonia-lyases Proteins 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 229910000365 copper sulfate Inorganic materials 0.000 abstract 1
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 abstract 1
- 229910000360 iron(III) sulfate Inorganic materials 0.000 abstract 1
- 229940099596 manganese sulfate Drugs 0.000 abstract 1
- MEFBJEMVZONFCJ-UHFFFAOYSA-N molybdate Chemical compound [O-][Mo]([O-])(=O)=O MEFBJEMVZONFCJ-UHFFFAOYSA-N 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 229910000368 zinc sulfate Inorganic materials 0.000 abstract 1
- 229960001763 zinc sulfate Drugs 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 36
- 238000012360 testing method Methods 0.000 description 33
- 239000007921 spray Substances 0.000 description 23
- 235000013339 cereals Nutrition 0.000 description 22
- 239000003337 fertilizer Substances 0.000 description 20
- 230000000694 effects Effects 0.000 description 19
- 230000012010 growth Effects 0.000 description 17
- 239000002689 soil Substances 0.000 description 16
- 240000007594 Oryza sativa Species 0.000 description 15
- 235000007164 Oryza sativa Nutrition 0.000 description 15
- 235000009566 rice Nutrition 0.000 description 15
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 235000003228 Lactuca sativa Nutrition 0.000 description 9
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 8
- 235000001014 amino acid Nutrition 0.000 description 8
- 229940095054 ammoniac Drugs 0.000 description 8
- 229940088598 enzyme Drugs 0.000 description 8
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 241000208822 Lactuca Species 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 230000008901 benefit Effects 0.000 description 7
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 6
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 230000004720 fertilization Effects 0.000 description 6
- 235000009973 maize Nutrition 0.000 description 6
- VLAPMBHFAWRUQP-UHFFFAOYSA-L molybdic acid Chemical compound O[Mo](O)(=O)=O VLAPMBHFAWRUQP-UHFFFAOYSA-L 0.000 description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 230000036512 infertility Effects 0.000 description 5
- 208000000509 infertility Diseases 0.000 description 5
- 231100000535 infertility Toxicity 0.000 description 5
- 230000035800 maturation Effects 0.000 description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 239000000575 pesticide Substances 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- 230000010152 pollination Effects 0.000 description 5
- 238000009331 sowing Methods 0.000 description 5
- 235000013311 vegetables Nutrition 0.000 description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- 102000003992 Peroxidases Human genes 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 108040007629 peroxidase activity proteins Proteins 0.000 description 4
- 239000011591 potassium Substances 0.000 description 4
- 229910052700 potassium Inorganic materials 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- 239000011701 zinc Substances 0.000 description 4
- 229910052725 zinc Inorganic materials 0.000 description 4
- ATROHALUCMTWTB-WYMLVPIESA-N (z)-n-diethoxyphosphinothioyloxybenzenecarboximidoyl cyanide Chemical compound CCOP(=S)(OCC)O\N=C(/C#N)C1=CC=CC=C1 ATROHALUCMTWTB-WYMLVPIESA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- 244000269722 Thea sinensis Species 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 210000003608 fece Anatomy 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 238000003973 irrigation Methods 0.000 description 3
- 230000002262 irrigation Effects 0.000 description 3
- 239000010871 livestock manure Substances 0.000 description 3
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 3
- 239000003053 toxin Substances 0.000 description 3
- 231100000765 toxin Toxicity 0.000 description 3
- 108010011619 6-Phytase Proteins 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- 240000008415 Lactuca sativa Species 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 235000014676 Phragmites communis Nutrition 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 229920005610 lignin Polymers 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 125000000018 nitroso group Chemical group N(=O)* 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000002686 phosphate fertilizer Substances 0.000 description 2
- 229950001664 phoxim Drugs 0.000 description 2
- 229940085127 phytase Drugs 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000001863 plant nutrition Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000005070 ripening Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 210000004894 snout Anatomy 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- WURBVZBTWMNKQT-UHFFFAOYSA-N 1-(4-chlorophenoxy)-3,3-dimethyl-1-(1,2,4-triazol-1-yl)butan-2-one Chemical compound C1=NC=NN1C(C(=O)C(C)(C)C)OC1=CC=C(Cl)C=C1 WURBVZBTWMNKQT-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241001530056 Athelia rolfsii Species 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 102000002568 Multienzyme Complexes Human genes 0.000 description 1
- 108010093369 Multienzyme Complexes Proteins 0.000 description 1
- 101000905241 Mus musculus Heart- and neural crest derivatives-expressed protein 1 Proteins 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 235000019082 Osmanthus Nutrition 0.000 description 1
- 241000333181 Osmanthus Species 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- JJEJDZONIFQNHG-UHFFFAOYSA-N [C+4].N Chemical compound [C+4].N JJEJDZONIFQNHG-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 238000000556 factor analysis Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000009746 freeze damage Effects 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 235000021552 granulated sugar Nutrition 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- UFHLMYOGRXOCSL-UHFFFAOYSA-N isoprothiolane Chemical compound CC(C)OC(=O)C(C(=O)OC(C)C)=C1SCCS1 UFHLMYOGRXOCSL-UHFFFAOYSA-N 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 229960005190 phenylalanine Drugs 0.000 description 1
- 235000008729 phenylalanine Nutrition 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000007198 pollen germination Effects 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical class [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 description 1
- 229910001950 potassium oxide Inorganic materials 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 150000004053 quinones Chemical class 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- JXOHGGNKMLTUBP-HSUXUTPPSA-N shikimic acid Chemical compound O[C@@H]1CC(C(O)=O)=C[C@@H](O)[C@H]1O JXOHGGNKMLTUBP-HSUXUTPPSA-N 0.000 description 1
- JXOHGGNKMLTUBP-JKUQZMGJSA-N shikimic acid Natural products O[C@@H]1CC(C(O)=O)=C[C@H](O)[C@@H]1O JXOHGGNKMLTUBP-JKUQZMGJSA-N 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000002426 superphosphate Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- QSOHVSNIQHGFJU-UHFFFAOYSA-L thiosultap disodium Chemical compound [Na+].[Na+].[O-]S(=O)(=O)SCC(N(C)C)CSS([O-])(=O)=O QSOHVSNIQHGFJU-UHFFFAOYSA-L 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a plant growth regulator and a method for producing the same. The plant growth regulator is characterized by comprising raw materials according to the following weight ratio: 1 to 20 weight portions of copper sulfate, 1 to 50 weight portions of zinc sulfate, 1 to 30 weight portions of manganese sulfate, 1 to 40 weight portions of ferric sulfate, 1 to 25 weight portions ofsodium molybdate, 0.1 to 1 weight portion of amino acid, 1 to 20 weight portions of nucleic acid protein, 0.001 to 0.002 weight portion of vitamin E, 2 to 6 weight portions of agricultural rare earth, 1 to 30 weight portions of silicic acid, 5 to 20 weight portions of sugar, and 1,000 to 2,000 weight portions of water, wherein each one hundred weight portions of the regulator contains 0.5 weight portion of potato polyphenol oxidase complex, 0.5 weight portion of rhodotorula glutinis L-phenylalanine ammonia-lyase complex, 0.5 weight portion of earthworm superoxide dismutase, 0.5 weight portionof seaweed bioactive substances, and 0.5 weight portion of anti-pest composite.
Description
Technical field
The present invention relates to a plant growth regulators and production method thereof, the present invention relates to a kind of composition that promotes that plant increases.The present invention both can fixed nitrogen, phosphorus decomposing, potassium decomposing; Decomposition of cellulose and organic acid; Can decompose phenol, H again
2S, nitroso; Secretion growth stimulant, antibiotic, multiple functional enzyme.It can satisfy plant nutrition, increases crop yield, improves quality, improves resistance, also can reduce the remaining toxin in the plant corpus simultaneously.
Technical background
In agricultural production, the main method that promotes the crops level of production to improve is to increase the needed fertilizer of plant growing, the application of single chemical fertilizer is played very big facilitation to modern agricultural development, but, use incorrect meeting to destroy soil structure, make edaphon very rare, organic matter decomposition is obstructed, nutritive element runs off, and has reduced the soil resource availability even has polluted well, river, lake because of loss.Especially partially nitrogen, and lack the cooperation of other elements, often make quality, resistance, the mouthfeel of crops all be subjected to bigger influence, nitrogen fertilizer application will be detrimental to health and environment partially.Crops nitrate enters in people's carcass carcinogenesis.Throughout the year a large amount of use chemical fertilizer easily causes hardening soil degree to improve constantly, cause grain, really, vegetable matter constantly descends, the shallow water resource is by pollution by chemical fertilizer, the content of organic matter in the soil is fewer and feweri, ecotope is damaged.In addition, agricultural products in China residue of pesticide problem is more serious.The producer uses in violation of rules and regulations high-toxic pesticide or excess to use the phenomenon ubiquity of agricultural chemicals on vegetable and fruit, causes that residue of pesticide exceed standard in many agricultural product.
There are more quality problems in the product of China's microbial manure production at present, and is low such as effective viable bacteria content, and assorted bacterium rate is higher, and the term of validity is short etc.Also have the formation of some products to show significantly unreasonable, for example in a kind of product, add tens kinds of microorganisms, this just exists a reproduction speed fast, slow, the problem in anti-adversity difference, contention nutrient, contention space, and how the bacterial classification that breeding amount is few works in soil.The bacterial classification difference, to making the effect difference of deposits yields, have to increase to have and subtract, the crop that has is effective, the crop that has is invalid substantially. the nutriment (comprising nitrogen, phosphorus, potassium and multiple mineral element) that bio-fertilizer can not directly provide crop to absorb, but make and assist crop absorption nutriment or produce the growth that somatotropin stimulates crop by the positive activity of microorganism in soil of a large amount of work.The chemical fertilizer or other additive that add underexercise in the bio-fertilizer that has make that nitrogen, phosphorus, the potassium content of chemical fertilizer in the fertilizer is too high to cause effective bacterium death, and lose the effect of bio-fertilizer.
Existing fertilizer all is that the mankind apply extraneous strength to plant, and has ignored oneself's immunity of plant itself, self-protection, and self-anti-poor environment, therefore the ability of oneself's supply nutrition, needs to propose a plant growth regulators.
Summary of the invention
The object of the present invention is to provide a plant growth regulators and production method thereof, elements such as the potato PPO compound that contains among the present invention, rhodotorula glutinis L-phenylalnine ammonialyase compound, earthworm superoxide dismutase, seaweed bio active substance, disease-resistant worm compound, phytase, copper, zinc, manganese, iron are the indispensable most important elements of plant health, and the sterilization of preventing and curing diseases of plant is played special effect; The present invention with plant this as this, from natural zooblast, plurality of active ingredients in the plant cell that plant is required extracts respectively, prepares as required according to different crops then, real high efficiency, the specific aim that realizes plant health care product, the present invention can fixed nitrogen, phosphorus decomposing, potassium decomposing; Decomposition of cellulose and organic acid; Decompose phenol, H
2S, nitroso; Secretion growth stimulant, antibiotic, multiple functional enzyme.It can satisfy plant nutrition, increases crop yield, improves quality, improves resistance, also can reduce the remaining toxin in the plant corpus simultaneously, the remains of pesticide on the crops of can effectively degrading.
The objective of the invention is to be realized by following technical proposals: a plant growth regulators comprises the following weight proportion raw material:
Copper sulphate 1-20
Zinc sulphate 1-50
Manganese sulphate 1-30
Iron sulfate 1-40
Molybdic acid is received 1-25
Amino acid 0.1-1
Nucleic acid-protein 1-20
Vitamin E 0.001-0.002
Agricultural rare-earth 2-6
Silicic acid 1-30
Sugar 5-20
Water 1000-2000;
Contain the potato PPO compound of 0.5 weight portion, the rhodotorula glutinis L-phenylalnine ammonialyase compound of 0.5 weight portion, the earthworm superoxide dismutase of 0.5 weight portion, the seaweed bio active substance of 0.5 weight portion, the disease-resistant worm compound of 0.5 weight portion in the described conditioning agent of per 100 weight portions.
Another object of the present invention is realized by following technical proposals: the production method of a plant growth regulators, comprise the following weight proportion raw material, copper sulphate 1-20, zinc sulphate 1-50, manganese sulphate 1-30, iron sulfate 1-40, molybdic acid are received 1-25, amino acid 0.1-1, nucleic acid-protein 1-20, vitamin E 0.001-0.002, agricultural rare-earth 2-6, silicic acid 1-30, sugared 5-20, water 1000-2000; Contain the potato PPO compound of 0.5 weight portion, the rhodotorula glutinis L-phenylalnine ammonialyase compound of 0.5 weight portion, the earthworm superoxide dismutase of 0.5 weight portion, the seaweed bio active substance of 0.5 weight portion, the disease-resistant worm compound of 0.5 weight portion in the described conditioning agent of per 100 weight portions;
The extraction step of I, rhodotorula glutinis L-phenylalnine ammonialyase compound:
A, preparation medium comprise following weight percentages in the medium: sucrose 1%, corn steep liquor 3%, earthworm slurry 2%, sodium chloride (NaCL) 0.5%, L-tyrosine 0.1%, dipotassium hydrogen phosphate (K
2HPO
4) 0.1%, defoamer 0.01%, all the other are clean waters, regulate the pH value 5.0～5.5 of medium;
B, medium is carried out sterilization processing, under 30 ℃ of temperature, inoculate bacterial classification then, under 30 ℃ of temperature, cultivated 12-15 hour, under 40 ℃ of temperature, cultivated 5-7 hour, generate zymotic fluid;
C, zymotic fluid is carried out centrifugal treating, collect wet cell;
D, working concentration are that 0.9% physiological saline carries out carrying out washing treatment to wet cell, add 1 gram wet cell in every ml physiological saline, then physiological saline are carried out centrifugal treating, collect wet cell;
E, wet cell and buffer solution are mixed into cell suspending liquid, add 1 gram wet cell in per 4 milliliters of buffer solutions; In cell suspending liquid, add concentration then again and be 10% triton x-100 osmotic treated; The concentration of described buffer solution is 50mmol/L, pH value 8.8;
F, use processor for ultrasonic wave are handled described cell suspending liquid ultrasonic disruption, produce broken liquid;
G, to described broken liquid multilayer filtered through gauze, produce filtered fluid, filtered fluid is carried out centrifugal treating, get supernatant;
H, select molecular cut off 20,000 PS milipore filters to carry out ultrafiltration and concentration to supernatant to handle, obtain once concentration liquid;
The degree of saturation scale of I, the actual volume according to once concentration liquid, temperature and sulfate of ammoniac calculates and reaches 38% degree of saturation and should add sulfate of ammoniac consumption in the concentrate, prepares out solid sulphuric acid iron;
J, once concentration liquid is directly poured in the beaker, added solid sulphuric acid iron, then once concentration liquid is carried out refrigerated centrifuge and handle, obtain the secondary supernatant;
K, to secondary supernatant ultrafiltration and concentration, obtain secondary concentration liquid;
The degree of saturation scale of L, the actual volume according to secondary concentration liquid, temperature and sulfate of ammoniac calculates from the required sulfate of ammoniac consumption of 38%-75% degree of saturation, prepares out solid sulphuric acid iron;
M, secondary concentration liquid is directly poured in the beaker, added solid sulphuric acid iron, then secondary concentration liquid is carried out refrigerated centrifuge and handle, abandoning supernatant keeps sediment, obtains rhodotorula glutinis L-phenylalnine ammonialyase compound;
N, with the freeze-drying of rhodotorula glutinis L-phenylalnine ammonialyase compound, preserve down at-20 ℃ then;
II, potato PPO (PPO) compound extraction step:
A, with potato clean, section, carry out homogenized then; In the homogenized process, add the 0.03M phosphate buffer of 1 liter of parts by volume in the potato pulp of per 1 kilogram-mass part, mix, obtain potato pulp; The temperature of described 0.03M phosphate buffer is 1 ℃, pH value 5.5;
After B, the homogenized, use the multilayer gauze that potato pulp is filtered, then filtered fluid is carried out centrifugation and handle, obtain the polyphenol oxidase zyme extract;
C, in the polyphenol oxidase zyme extract, add solid ammonium sulfate and make it reach 40% degree of saturation, carry out centrifugation then and handle, obtain supernatant;
D, in supernatant, add solid ammonium sulfate and make it reach 70% degree of saturation, carry out centrifugation then and handle, collect thick enzyme sediment, obtain the potato PPO compound;
The extraction step of III, earthworm superoxide dismutase:
A, the earthworm that will live place light salt brine to clean, and the content of salt is no more than 0.3% in the light salt brine; The earthworm that will live is rubbed processing, and in the rubbing process, the lactose that adds about 0.5 vitamin C that restrains and 0.5 gram in per 1000 gram earthworms obtains earthworm juice as protectant;
B, earthworm juice is obtained earthworm freeze after freezing processing under-4 ℃～-1 ℃ the temperature;
C, earthworm frozen put into the phosphate buffer homogenized, the earthworm of per 1 kilogram-mass part is frozen the phosphate buffer of 3 liters of parts by volume of configuration, and the parameter of phosphate buffer is 0.05mol/L, pH value 7.8, and homogenized finishes, carry out centrifugation and handle, obtain one time supernatant;
D, supernatant is used the multilayer filtered through gauze, then filtered fluid is carried out centrifugation and handle, obtain the secondary supernatant;
E, will be in the above-mentioned clear liquid of secondary add the acetone mixed processing of 1.25 times of parts by volume, leave standstill processing after, the collecting precipitation thing;
F, described sediment joined in the phosphate buffer dissolve, obtain lysate; The phosphate buffer of 3 liters of parts by volume of sediment configuration of per 1 kilogram-mass part, the parameter of phosphate buffer is 0.05mol/L, pH value 7.8;
G, to the water-bath heating 20 minutes under 65 ℃ of conditions of described lysate, left standstill 10 minutes, water-bath is heated to 65 ℃ again, be cooled to 4 ℃ then rapidly, lysate is carried out centrifugation handle, obtain three times supernatant, contain the earthworm superoxide dismutase in this supernatant;
H, the earthworm superoxide dismutase is preserved under-4 ℃ of conditions;
IV, seaweed bio active substance extraction step:
A, get 1 kilogram of clean marine alga, add 1 kilogram of wash clean earthworm, add one piece of raw egg and carry out homogenized, obtain mixed serum;
B, by per 1 kilogram of mixed serum add sucrose 40 grams, dusty yeast 20 grams, ammonium sulfate 1.0 grams, 1000 gram water stir the back, be warmed up to 40 ℃, fermentation process obtains zymotic fluid;
C, the 3mol/L phosphate buffer that adds 5 times of volumes in described zymotic fluid mix, and are heated to 100 ℃ of insulations 20-48 hour, obtain mixed solution;
D, the mixed solution temperature is cooled to normal temperature, the pH value of adjusting mixed solution with ammoniacal liquor reaches between the 6-7 then, and acquisition seaweed bio active substance extract is preserved under-4 ℃ of conditions;
V, disease-resistant worm compound extraction step:
A, balsam pear, ginger, garlic, rape flower, ginkgo skin, tree-of-heaven, fish Teng, green peel of walnut, castor bean leaf, bunge pricklyash leaf, tarragon are cleaned up with clear water, dry then;
B, above-mentioned raw materials is prepared burden by weight, mix then to pulverize and form crushed material, wherein, 10 parts in balsam pear, 3 parts in ginger, 7 parts in garlic, 10 parts of rape flowers, 10 parts of ginkgo skins, 10 parts of tree-of-heavens, 10 parts of fish Tengs, 10 parts of green peel of walnut, 10 parts of castor bean leaves, 10 parts of bunge pricklyash leafs, 10 parts of tarragons;
C, with the water that crushed material adds 5 times of parts by weight, add 1 ‰ phosphoric acid again, under 100 ℃ of conditions boiling 0.5-1 hour, soak then and placed 5-8 hour, obtain disease-resistant worm compound soup;
D, enantiopathy worm compound soup use the multilayer filtered through gauze, filtering liquid medicine is carried out centrifugation handle, and collect supernatant;
E, the pH value of using ammoniacal liquor to adjust supernatant reach 6.5-7, obtain disease-resistant worm compound, and disease-resistant worm compound is preserved under-4 ℃ of conditions.
Compared with the prior art the present invention has following advantage:
1, elements such as the copper that contains among the present invention, zinc, manganese, iron, rare earth, vitamin, carbohydrate are the indispensable most important elements of plant health, and the sterilization of preventing and curing diseases of plant is played special effect.
2, the superoxide dismutase that contains among the present invention (SOD) has synergistic effect to the nutritive element of plant absorbing, can improve resistance against diseases and the defence capability of crops greatly.
3, the superoxide dismutase that contains among the present invention, phytase, the agricultural rare-earth remains of pesticide on the crops of can effectively degrading.
Embodiment
Embodiment one:
One plant growth regulators, it comprises the following weight proportion raw material, is measurement unit with the gram:
Copper sulphate (in copper) 1
Zinc sulphate (in zinc) 1
Manganese sulphate (in manganese) 1
Iron sulfate (in iron) 1
Molybdic acid is received (in molybdenum) 1
Amino acid 0.1
Nucleic acid-protein 1
Vitamin E 0.001
Agricultural rare-earth 2
Silicic acid (in silicon) 1
Sugar (industrial granulated sugar) 5
Water (clean water) 1000;
Contain the potato PPO compound of 0.5 gram, the rhodotorula glutinis L-phenylalnine ammonialyase compound of 0.5 gram, the earthworm superoxide dismutase of 0.5 gram, the seaweed bio active substance of 0.5 gram, the disease-resistant worm compound of 0.5 gram in the described conditioning agent of per one hectogram.
The polyphenol oxidase multienzyme complex that uses in the present embodiment, phenylalnine ammonialyase compound, superoxide dismutase, seaweed bio active substance, disease-resistant worm compound extracting method can use embodiment four disclosed methods, are not described in detail at this.
White sugar among the present invention is hydrocarbon, can promote the absorption to each trace elements of plant.Elements such as the copper that the present invention contains, zinc, manganese, iron, rare earth, vitamin, carbohydrate are the indispensable most important elements of plant health, and the sterilization of preventing and curing diseases of plant is played special effect.All plants that contain Ye Lvjia are all worked, crops and flue-cured tobacco, fruit tree, tealeaves etc. all have good effect of increasing production widely to vegetables, paddy rice, corn etc., it is improved about output 20%-50%, the piece root crop is increased production about 50%-80%, and low temperature cold current, overcast and rainy low light photograph and arid heat wave etc. are had functions such as tangible resistance, defence.
In the present embodiment, employed polyphenol oxidase (PPO), phenylalnine ammonialyase phenylalaninammo-nialyase (PAL), peroxidase (PO) belong to the defensive ferment of plant; Employed superoxide dismutase (Superoxide Dismutase is called for short SOD) is to remove the important enzyme .SOD of oxygen radical and the activity of CAT in the organism, reduce MDA content and membrane permeability, improving chlorophyll mass fraction and root system vigor has and is significantly just imitating .SOD and can improve crop anti-adversity, is playing a very important role aspect disease, freeze injury, aging, arid and the storage endurance.PAL is the constant speed enzyme of shikimic acid metabolic pathway, is the key enzyme in the antibacterial material building-up processes such as phenol, protective plant protecting agent, lignin.The effect synthetic and heteroauxin of PO and ethene is relevant, and two kinds of hormones can influence the great change of metabolism, and influences the metabolism of phenol with PO.Itself has disease resistance phenol, and its oxidation quinones sterilizing power is stronger, can the oxidation ectodesm and cause the pathogen expansion to be obstructed, cause the irritated resistance reaction of plant; The phenol material can also form the lignin precursor, makes the host cell lignification and disease-resistant.Even PPO itself also has inhibitory action to plant disease.
Occupation mode of the present invention is to use spraying apparatus to be sprayed directly on the blade face of crops.
Embodiment two:
Present embodiment is the preferred version on embodiment one basis, and it comprises the following weight proportion raw material, is measurement unit with the gram:
Copper sulphate 10
Zinc sulphate 25
Manganese sulphate 15
Iron sulfate 20
Molybdic acid receives 12
Amino acid 0.5
Nucleic acid-protein 10
Vitamin E 0.0015
Agricultural rare-earth 4
Silicic acid 20
Sugar 12
Water 1500.
Embodiment three:
Present embodiment is the preferred version on embodiment one basis, and it comprises the following weight proportion raw material, is measurement unit with the gram:
Copper sulphate 20
Zinc sulphate 50
Manganese sulphate 30
Iron sulfate 40
Molybdic acid receives 25
Amino acid/11
Nucleic acid-protein 20
Vitamin E 0.002
Agricultural rare-earth 6
Silicic acid 30
Sugar 20
Water 2000.
Embodiment four:
The production method of one plant growth regulators, comprise the following weight proportion raw material, copper sulphate 1-20, zinc sulphate 1-50, manganese sulphate 1-30, iron sulfate 1-40, molybdic acid are received 1-25, amino acid 0.1-1, nucleic acid-protein 1-20, vitamin E 0.001-0.002, agricultural rare-earth 2-6, silicic acid 1-30, sugared 5-20, water 1000-2000; Contain the potato PPO compound of 0.5 weight portion, the rhodotorula glutinis L-phenylalnine ammonialyase compound of 0.5 weight portion, the earthworm superoxide dismutase of 0.5 weight portion, the seaweed bio active substance of 0.5 weight portion, the disease-resistant worm compound of 0.5 weight portion in the described conditioning agent of per 100 weight portions;
The extraction step of I, rhodotorula glutinis L-phenylalnine ammonialyase compound:
A, preparation medium comprise following weight percentages in the medium: sucrose 1%, corn steep liquor 3%, earthworm slurry 2%, sodium chloride (NaCL) 0.5%, L-tyrosine 0.1%, dipotassium hydrogen phosphate (K
2HPO
4) 0.1%, defoamer 0.01%, all the other are clean waters, regulate the pH value 5.0～5.5 of medium;
B, medium is carried out sterilization processing, under 30 ℃ of temperature, inoculate bacterial classification then, under 30 ℃ of temperature, cultivated 15 hours, under 40 ℃ of temperature, cultivated 7 hours, generate zymotic fluid; Conventional equipment is used in sterilization;
C, zymotic fluid was carried out centrifugal treating 7 minutes, collect wet cell, the centrifugal separator rotating speed is 5000rpm;
D, working concentration are that 0.9% physiological saline carries out carrying out washing treatment to wet cell, add 1 gram wet cell in every ml physiological saline, then physiological saline are carried out centrifugal treating 7 minutes, collect wet cell, and the centrifugal separator rotating speed is 5000rpm;
E, wet cell and buffer solution are mixed into cell suspending liquid, add 1 gram wet cell in per 4 milliliters of buffer solutions; In cell suspending liquid, added concentration (quality volumetric concentration) then again and be 10% triton x-100 (TrionX-100) osmotic treated 7 hours; The concentration of described buffer solution is 50mmol/L, pH value 8.8; Described buffer solution is Pehanorm-hydrochloride buffer (Tris-HCL);
F, use processor for ultrasonic wave were handled 25 minutes described cell suspending liquid ultrasonic disruption under 850W power, produced broken liquid;
G, to described broken liquid with three layers of filtered through gauze, produce filtered fluid, filtered fluid was carried out centrifugal treating 15 minutes, get supernatant (containing rhodotorula glutinis L-phenylalnine ammonialyase compound in the supernatant), the centrifugal separator rotating speed is 5000rpm;
H, select molecular cut off 20,000 PS milipore filters to carry out ultrafiltration and concentration to supernatant to handle, obtain once concentration liquid;
The degree of saturation scale of I, the actual volume according to once concentration liquid, temperature and sulfate of ammoniac calculates and reaches 38% degree of saturation and should add sulfate of ammoniac consumption in the concentrate, prepares out solid sulphuric acid iron;
J, once concentration liquid is directly poured in the beaker, the limit is slowly stirred the limit and is slowly added the solid sulphuric acid iron that weighs up, and after treating all to add dissolving, slowly stirs 10 minutes again; Then once concentration liquid is carried out refrigerated centrifuge and handled 10 minutes, obtain the secondary supernatant, centrifugal rotational speed is 5000rpm, and temperature condition is-4 ℃;
K, to secondary supernatant ultrafiltration and concentration, obtain secondary concentration liquid;
The degree of saturation scale of L, the actual volume according to secondary concentration liquid, temperature and sulfate of ammoniac calculates from the required sulfate of ammoniac consumption of 38%-75% degree of saturation, prepares out solid sulphuric acid iron;
M, secondary concentration liquid is directly poured in the beaker, the limit is slowly stirred the limit and is slowly added the solid sulphuric acid iron that weighs up, and after treating all to add dissolving, slowly stirs 10 minutes again; Then secondary concentration liquid is carried out refrigerated centrifuge and handled 10 minutes, abandoning supernatant keeps sediment, obtains rhodotorula glutinis L-phenylalnine ammonialyase compound, and centrifugal rotational speed is 5000rpm, and the temperature condition of refrigerated centrifuge is-4 ℃;
N, with the freeze-drying of rhodotorula glutinis L-phenylalnine ammonialyase compound, preserve down at-20 ℃ then;
II, potato PPO (PPO) compound extraction step:
A, with potato clean, section, use the plant tissue refiner to carry out homogenized then; In the homogenized process, add the 0.03M phosphate buffer of 1 liter of parts by volume in the potato pulp of per 1 kilogram-mass part, mix, obtain potato pulp; The temperature of described 0.03M phosphate buffer is 1 ℃, pH value 5.5, comprises EDTA, 5% glycerine, 1% polyvinylpyrrolidone of 0.02M mercaptoethanol, 0.001M in the described 0.03M phosphate buffer;
After B, the homogenized, use 4 layers of gauze that potato pulp is filtered, then filtered fluid is carried out centrifugation and handle, obtain the polyphenol oxidase zyme extract; The centrifugal separator rotating speed is 6000rpm, centrifugal treating 10 minutes;
C, in the polyphenol oxidase zyme extract, add solid ammonium sulfate and make it reach 40% degree of saturation, stir while adding and reach abundant dissolving, carry out centrifugation then and handle, obtain supernatant; The centrifugal separator rotating speed is 6000rpm, centrifugal treating 20 minutes;
D, add solid ammonium sulfate make it reach 70% degree of saturation in supernatant, carry out centrifugation then and handle, the reject supernatant is collected thick enzyme sediment, obtains the potato PPO compound; The centrifugal separator rotating speed is 6000rpm, centrifugal treating 20 minutes;
The extraction step of III, earthworm superoxide dismutase:
A, the earthworm that will live place light salt brine to clean three times, and the content of salt is no more than 0.3% in the light salt brine; The earthworm that will live is rubbed processing, and in the rubbing process, the lactose that adds about 0.5 vitamin C that restrains and 0.5 gram in per 1000 gram earthworms obtains earthworm juice as protectant;
B, earthworm juice is obtained earthworm freeze after freezing processing under-4 ℃～-1 ℃ the temperature;
C, earthworm frozen put into the phosphate buffer homogenized, the earthworm of per 1 kilogram-mass part is frozen the phosphate buffer of 3 liters of parts by volume of configuration, the parameter of phosphate buffer is 0.05mol/L, pH value 7.8, continuous stirring 1～2 hour, homogenized finishes, and carries out centrifugation and handles, and obtains one time supernatant, the centrifugal separator rotating speed is 15000rpm, centrifugal treating 30 minutes;
D, supernatant is used 4 layers of filtered through gauze, then filtered fluid is carried out centrifugation and handle, obtain the secondary supernatant, the centrifugal separator rotating speed is 6000rpm, centrifugal treating 20 minutes;
E, with adding the acetone mixed processing of 1.25 times of parts by volume in the above-mentioned clear liquid of secondary, left standstill the collecting precipitation thing 1 hour;
F, described sediment joined in the phosphate buffer dissolve, obtain lysate; The phosphate buffer of 3 liters of parts by volume of sediment configuration of per 1 kilogram-mass part, the parameter of phosphate buffer is 0.05mol/L, pH value 7.8;
G, to the water-bath heating 20 minutes under 65 ℃ of conditions of described lysate, left standstill 10 minutes, water-bath is heated to 65 ℃ again, be cooled to 4 ℃ then rapidly, lysate is carried out centrifugation to be handled, obtain three times supernatant, contain earthworm superoxide dismutase (earthworm superoxide dismutase-peroxidase complex enzyme) in this supernatant; The centrifugal separator rotating speed is 6000rpm, centrifugal treating 20 minutes;
H, the earthworm superoxide dismutase is preserved under-4 ℃ of conditions;
IV, seaweed bio active substance extraction step:
A, get 1 kilogram of clean marine alga, add 1 kilogram of wash clean earthworm, add one piece of raw egg and carry out homogenized, obtain mixed serum;
B, by per 1 kilogram of mixed serum add sucrose 40 grams, dusty yeast 20 grams, ammonium sulfate 1.0 grams, 1000 gram water stir the back, be warmed up to 40 ℃, fermentation time 84 hours obtains zymotic fluid;
C, the 3mol/L phosphate buffer that adds 5 times of volumes in described zymotic fluid mix, and are heated to 100 ℃ of insulations 48 hours, obtain mixed solution;
D, the mixed solution temperature is cooled to normal temperature, the pH value of adjusting mixed solution with ammoniacal liquor reaches between the 6-7 then, and acquisition seaweed bio active substance extract is preserved under-4 ℃ of conditions;
V, disease-resistant worm compound extraction step:
A, balsam pear, ginger, garlic, rape flower, ginkgo skin, tree-of-heaven, fish Teng, green peel of walnut, castor bean leaf, bunge pricklyash leaf, tarragon are cleaned up with clear water, dry then;
B, above-mentioned raw materials is prepared burden by weight, mix then to pulverize and form crushed material, wherein, 10 parts in balsam pear, 3 parts in ginger, 7 parts in garlic, 10 parts of rape flowers, 10 parts of ginkgo skins, 10 parts of tree-of-heavens, 10 parts of fish Tengs, 10 parts of green peel of walnut, 10 parts of castor bean leaves, 10 parts of bunge pricklyash leafs, 10 parts of tarragons;
C, with the water that crushed material adds 5 times of parts by weight, add 1 ‰ phosphoric acid again, boiling is 1 hour under 100 ℃ of conditions, soak to place then 8 hours, obtains disease-resistant worm compound soup;
D, enantiopathy worm compound soup use 4 layers of filtered through gauze, filtering liquid medicine is carried out centrifugation handle, and collect supernatant; The centrifugal separator rotating speed is 6000rpm, centrifugal treating 10 minutes;
E, the pH value of using ammoniacal liquor to adjust supernatant reach 6.5-7, obtain disease-resistant worm compound, and disease-resistant worm compound is preserved under-4 ℃ of conditions.
In the present embodiment, above-mentioned raw materials is got all the ready, mixed according to common process and get final product.
Conditioning agent of the present invention detects through China Guangzhou Analysis ﹠. Test Center, and testing result is as follows:
| Analysis project | Testing result | Measurement unit |
| Aspartic acid (Asp) | 8.0 | mg/100g |
| Glutamic acid (Glu) | 13.7 | |
| Serine (Ser) | 6.9 | |
| Histidine (His) | 1.3 | |
| Glycine (Gly) | 6.2 | |
| Threonine (Thr) | 4.5 | |
| Arginine (Arg) | 7.0 | |
| Alanine (Ala) | 10.0 | |
| Tyrosine (Tyr) | 1.1 | |
| Valine (Val) | 4.8 | |
| Methionine (Met) | Do not detect |
| Phenyl alanine (Phe) | 1.2 | |
| Isoleucine (Ile) | 1.9 | |
| Leucine (Leu) | 3.4 | |
| Lysine (Lys) | 1.6 | |
| Proline (Pro) | 6.8 | |
| The summation of above free amino acid (Total Free Amino Acids) | 78 | |
| Bata-carotene (β-carolene) | 0.19 | μg/100g |
| Superoxide dismutase (SuperOxide Dismutase) | 5.98×10 3 | U/ml |
The present invention experimentizes through Sichuan Academy of Agricultural Sciences's soil investigation, and the result is as follows:
One, test site
Paddy rice: the wise 6 society's gold-tinted all responsibilitys in the Lu Jia of Jianyang City town appoint with Pengzhou City Gexie Mountain town 7 Wang Zhi of society and contracted field in.
Corn: wise village, the Lu Jia of Jianyang City town 6 society's gold-tinted all responsibilitys appoint ground.
Lettuce: 2 groups of Wang Zhi of Pengzhou City Gexie Mountain town 7 societies and contracted field in.
Tealeaves: tea plantation, Qinglong Zhen Yu Tai village, Pengshan County.
Two, for examination soil
Paddy rice: reed young shoot of a reed wise 6 society's gold-tinted all responsibilitys in Jianyang City's are the paddy soil of purple soil parent material development with appointing, the 2 groups of Wang Zhi in 7 villages, Pengzhou City Gexie Mountain town and contracted field be the paddy soil of new alluviation parent material development.
Corn: wise village, the Lu Jia of Jianyang City town 6 society's gold-tinted all responsibilitys are the dryland soil of purple soil parent material development with appointing.
Lettuce: the 2 groups of Wang Zhi in 7 villages, Pengzhou City Gexie Mountain town and contracted field be the paddy soil of new alluviation parent material development.
Tealeaves: tea plantation, Qinglong Zhen Yu Tai village, Pengshan County is a hilly upland, and soil is yellow earth.
Above soil fertility is medium, irrigation and drainage good two season field or nonirrigated farmland, previous crops rape or wheat.
Three, experimental scheme:
1, cK 2, (mu is executed clear water 50kg) 3, the present invention's (mu is executed 100 times of liquid 50kg) 4, potassium dihydrogen phosphate (mu is executed 150 times of liquid 50kg)
Four, test is implemented:
(1), paddy rice
Test region, Jianyang City
District's group arrangement is at random adopted in test, triplicate, and sub-district area 0.027-0.003 mu, the aisle is 0.5 meter between repetition, and the aisle is 0.25 meter between processing, establishes guard row more than 1 meter all around.
1, experimental cultivar: suitable fragrant 3003.
2, the seedling raising and transplanting time, 200X grew seedlings March 25, and May 16 transplanted.
3, planting density: capable transplantings of width adopted in test, and wide capable 36cm, narrow row 18cm, nest be apart from 15cm, and then every sub-district plants the nest/district of 10 row/districts * 36 nests/OK=360, amounts to and mu plants 1.33 ten thousand nests.
4, Fertilization Level: 0.58 kilogram in urea is executed in every sub-district, 1.62 kilograms of superphosphate, 0.27 kilogram in potassium chloride.
5, field management: spray fertilizer once by test requirements document respectively at seedling stage April 16, spray fertilizer once tillering stage April 29, and shooting stage June 25 spray fertilizer once.And add phoxim control snout moth's larva of rice secondary with dimehypo respectively May 18 and July 18.
The test region, Pengzhou City
Small plot experiment is provided with 3 repetitions, and district's group is arranged at random.5.0 meters of zone leaders, wide 4.0 meters, 20 square metres of areas, between repetition, the aisle, minizone is 50cm, seal stays guard row in week.
1, kind: D excellent 63
2, the seedling raising and transplanting time: 200X sowed April 5, and May 12 transplanted.
3, planting density: transplant specification and be (10+6) very little * 4 cun, every nest is planted individual plant, every sub-district plants 600 strains, mu plants 2.0 ten thousand nests.
4, Fertilization Level: mu is made base fertilizer for 40 kilograms with 25% compound fertilizer dedicated to paddy, transplants back 7 days mu and topdresses once for 20 kilograms with the carbon ammonium.
5, field management: rice striking root phase, shooting stage, blooming stage mu are converted clear water with 200ml conditioning agent of the present invention and are sprayed for 40 kilograms, spray altogether three times.
After transplanting is turned green, the fine shallow irrigation that adopts, low temperature overcast and rainy day weather is with the Tian Weizhu that dries in the air, and the heading filling stage is based on intermittent irrigation.Seedling stage, booting stage, each prevented and treated rice blast once with Fujione.
(2), corn
Small plot experiment is provided with 3 repetitions, and district's group is arranged at random.6.7 meters * 3.0 meters=20.1 meters of every zone leaders
2=0.03 mu.Stay 50 centimetres in aisle between repetition, every sub-district kind 4 row, every row 25 strains, 100 strains of every district.
Establish guard row all around.
1, corn variety: become single 26
2, seedling raising and transplanting: 200X grew seedlings March 22.April 2 transplanted.
3, planting density: adopt the cultivation of single file individual plant, line-spacing 2.25 chis, nest are apart from 0.80 chi, and mu is planted 3333 strains.
4, Fertilization Level: 50 kilograms in urea of mu, 50 kilograms in phosphate fertilizer, farmyard manure 30 is chosen.Phosphate fertilizer is made base fertilizer entirely, and the urea bottom application accounts for 30% (0.45 kilogram/district), carries seed manure and accounts for 20% (0.3 kilogram/district), attacks luxuriant fertilizer and accounts for 50% (0.75 kilogram/district).
5, field management: conditioning agent of the present invention is by test requirements document, April 16 seedling spraying for the first time, shooting stage May 2 sprays for the second time, typhon mouth phase May 15 sprays for the third time.In May 20 with phoxim baythion control of maize snout moth's larva once.
(3), lettuce
Small plot experiment is provided with 4 and handles 3 repetitions, and district's group is arranged at random.Be provided with 4 and handle 3 repetitions, district's group is arranged at random, and 6.67 meters of zone leaders are wide 3.0 meters, 20.0 square metres of areas.Every sub-district plants 180 strains, and specification is 1.0 chis * 1.0 chis, amounts to mu and plants 6000 strains.Between processing, handle between, repeat between the aisle be 50 centimetres, stay guard row all around, select the transplantation of seedlings of ad eundem lettuce.Gathering in the crops note separately by the sub-district produces.
1, kind: the blue or green silk of hanging
2, management: carry out according to the land for growing field crops high-yield culture technique.Conditioning agent of the present invention is converted clear water and is sprayed for 40 kilograms, transplants back 10 days, opening quotation phase, expanding stage and respectively sprays once.
(4), tealeaves
Test is provided with three processing, three repetitions, and the sub-district area is 8 * 1.3m.200X sprayed for the first time April 26, investigation on May 29.For the second time spray May 29 the same year, investigation on June 25.Because of this year arid is serious, can not pluck again later on, test there is certain influence.
Four, result of the test
(1), rice test result
Two rice test results find out: use conditioning agent of the present invention and have good effect.Jianyang City is than 39.5 kilograms of spray clear water volume increase, and volume increase 11.51% is increased production 21.0 kilograms than spraying potassium dihydrogen phosphate, and rate of growth is 5.81%.
The paddy rice result of Pengzhou City compares according to 47.8 kilograms of volume increase, volume increase 10.62%; Than 36.7 kilograms of spray clear water volume increase, rate of growth is 7.96%, and than spraying 14.5 kilograms of potassium dihydrogen phosphate volume increase, rate of growth is 3.00%.
The survey showed that: spray the percentage of earbearing tiller that helps improving paddy rice behind the conditioning agent of the present invention, increase grain number per spike and thousand kernel weight.
(2), corn result of the test
The corn result of the test of Jianyang City is found out: using conditioning agent of the present invention also has good effect of increasing production, and than 41.0 kilograms of spray clear water volume increase, rate of growth is 10.81%, and than 24.6 kilograms of spray potassium dihydrogen phosphate volume increase, rate of growth is 6.22%.
The survey showed that in test: spray the grain number per spike and the 100-grain weight that help increasing corn behind the conditioning agent of the present invention.
(3), lettuce result of the test
The lettuce result of the test of Pengzhou City is found out: using conditioning agent of the present invention also has good effect of increasing production, and comparison is according to 306 kilograms of mu volume increase, volume increase 14.62%; Than 268 kilograms of spray clear water mu volume increase, volume increase 12.58%, than 88 kilograms of spray potassium dihydrogen phosphate mu volume increase, rate of growth is 3.81%.
The survey showed that: spray the growth that helps lettuce behind the conditioning agent of the present invention, individual plant weight and commodity individual plant weight all increase to some extent.
(3), tealeaves result of the test
Pengshan County tealeaves result of the test is found out: use conditioning agent of the present invention and help tealeaves increase bud tip quantity and improve hundred bud weight, thereby improve output, increase production 6.38% than spray clear water mu, than spray nutrient volume increase 2.78%.
Conclusion
1, rice application conditioning agent of the present invention has good effect.The Pengzhou City comparison is increased production 38.1 kilograms/mu of 10.62%: two some average specific spray clear water volume increase according to 47.8 kilograms/mu of volume increase, volume increase 9.74%, and than 17.75 kilograms/mu of application of phosphoric acid potassium dihydrogen volume increase, the training productive rate is 4.41%.
2, corn is used conditioning agent of the present invention, and than 41.0 kilograms/mu of spray clear water volume increase, rate of growth is 10.81%, and than 24.6 kilograms/mu of application of phosphoric acid potassium dihydrogen volume increase, rate of growth is 6.22%.
3, lettuce is used conditioning agent of the present invention, and comparison increases production 14.62% according to 306 kilograms/mu of volume increase; Than 268 kilograms/mu of spray clear water volume increase, volume increase 12.58%, than 88 kilograms/mu of application of phosphoric acid potassium dihydrogen volume increase, rate of growth is 3.81%.
4, conditioning agent of the present invention helps improving the percentage of earbearing tiller of paddy rice, and it is heavy with grain to increase grain number per spike.
5, conditioning agent of the present invention helps the growth of lettuce and improves individual plant weight and commodity individual plant weight.
6, conditioning agent of the present invention helps the increase of tea bud tip quantity and improves hundred bud weight, thereby improves output.
The present invention experimentizes through Dayi County rural development office, and the result is as follows:
One, basic condition
(1) place and scale
1, test: with the ground comparative trial be distributed in out Jiang Zhenxiang osmanthus village 12 societies (2 groups), go out Jiang Zhen vegetable garden village 12 societies (2 groups), Mu Jiaying village, He Ming township 13 societies (2 groups), totally 6 groups, 3.2 mu of test area are wherein handled 1.83 mu.
2, demonstration: Maize Production use exemplary distribution former in the Shanxi, please, the crane ring, go out 4 small towns, river, 52 mu of areas.
(2) experimental cultivar and breeding time
1, test: the corn variety for examination is respectively " Great Wall 799 " 3 groups 1.6 mu, April 2～20 sowing, August 2～5 maturation, average 116 days of the time of infertility; " become single 21 " 1 group 0.3 mu, sowing on March 27, August 1 maturation, the 128 days time of infertility; " refined beautiful No. 10 " 1 group 1 mu, March 27 sowing, August 1 maturation, the 134 days time of infertility; " difficult to understand beautiful No. 13 " 1 group 0.3 mu, sowing on March 27, August 15 maturation, the 132 days time of infertility.
2, demonstration: kind has " refined beautiful No. 10 ", " river single 15 ", " No. 2 at prime ", " Great Wall 799 ", " Dongdan 60 " etc., the 25-4 month in March 30 sowing, 28-August 15 July maturation, average 130 days of the time of infertility.
(3) fertilising situation
Execute 12.5 kilograms of purity nitrogens with ground comparative trial mu is flat, 8 kilograms of phosphorus pentoxides, 6.5 kilograms of potassium oxides, demonstration ground fertilizing amount can be represented the Fertilization Level of whole county Maize Production fully with experimental field basic identical.
Two, materials and methods
(1) with the ground comparative trial
1, time of application and number of times: according to maize growth process and " paddy rice, wheat, corn are used " failing to give attention to " plant growth regulator testing program " design, respectively in corn seedling stage and pollination phase application in two dressings.The concrete time is: on May 14,15 seedling stage, the pollination phase is 10-23 day in June.
2, amount of application and concentration: seedling stage and pollination phase all use by 1: 100 times of liquid, and mu is converted 50 kilograms in water.3, be used to shake the formula atomizer spray.
(2) demonstration
Time of application, number of times, consumption, concentration and method etc. are with identical with the ground comparative trial.
Three, test results and analysis
(1) experimental yield result
Find out that from the output statistics contrast processing average yield per mu of using plant growth regulator of the present invention reaches 410.96 kilograms, comparison is according to 356.14 kilograms of increases by 54.82 of (CK), volume increase 15.39%.6 groups is group group volume increase with the ground comparative trials, the amplitude maximum reach 133.5 kilograms, volume increase 38.36%; Increase production minimum reach 30 kilograms, volume increase 11.54%.By the hypothesis test of paired data, t=4.889＞t0.01-4.032.Illustrate to handle to have real difference that processing has extremely significant effect of increasing production than CK with CK.
The demonstration effect of increasing production with fit like a glove with the ground comparative test result, do not repeat comparison (down with) at this.
(2) volume increase factor analysis
Find out that from three factor analyses that spike number, a number and the heavy corn yield of grain constitute handle the average mu of spike number that produces effect and reach 2946.8, maintain an equal level with CK: single grain number per spike reaches 422.8, increases by 327.43 grams than CK, increases by 39.07 than CK, increase by 10.18%; Thousand kernel weight reaches 327.43 grams, increases by 16.02 grams than CK, increases by 5.14%.From a grain number and an effect of heavily increasing production,, grain number per spike accounts for 65.89% of total units increased in production because of increasing production 36.78 kilograms (theoretical calculating, down together); Increase production 19.04 kilograms because of thousand kernel weight, account for 34.1% of total units increased in production.Analysis result explanation: it is the increase that comes from grain number per spike that nearly 2/3rds production-increasing function is arranged, 1st/3rd, because the raising of thousand kernel weight, and spike number does not have notable difference.
(3) increasing yield initial analysis
Because plant growth regulating of the present invention is the conditioning agent of a kind of medicine, fertile dual-purpose type, just corn growth has been produced active influence since seedling stage.At first be to use conditioning agent seedling stage, the nutriment in the middle of the conditioning agent that made Maize Absorption, well developed root system, the leaf look dark green, the suction fertilizer of crop, water absorbing capacity and photosynthesis ability, and disease-resistant (northern and southern leaf blight, banded sclerotial blight) ability strengthens greatly, guaranteed the normal differentiation of male and female flowering, and striving for high yield for big fringe of later stage lays the foundation.Secondly, be to use taking out the hero phase of weaving silk, help the pollination of normally blooming, promote pollen germination and pollen tube growth, prolong the pollen life-span, improve the fertilization ripening rate, realize increasing grain and increase production.According to the species test data analysis, why great disparity is very big for grain number per spike, and reason is not that tassel row number increases (handle with CK and be 14.1 row), but row grain digital display work increases (be treated to 29.98, increase by 2.9 than CK, increase by 10.71%).In addition, the pollination phase uses the sea and helps transfer and the result of photosynthetic product to seed, promotes full seed, and grain heavily increases.
Four, benefit analysis
(1) economic benefit
Calculate by actual production, handling than CK mu is flat increases by 54.82 kilograms, and by 1.4 yuan of calculating of market price per kilogram, mu is flat to increase income 76.75 yuan.Remove 15 yuan of conditioning agent costs (2 times, totally 1000 milliliters), the peasant uses and throws 1 15 yuan of workers, and the flat net income of mu can reach 46.75 yuan, goes into operation than reaching 1: 2.56.The economic benefit highly significant.
(2) social benefit
Grain security is the cardinal task of our country, and corn is the third-largest cereal crops in the county, whole county cereal crops, occupies critical role in the production.Test, the demonstration proof is used " failing to give attention to " plant growth substance and have significant effect of increasing production on Maize Production.By more than 50 mu of calculating of this year demonstration, can increase kilogram surplus the corn 2700, if on more than the 50000 mu of corn in the whole county, generally apply, can increase more than 250 ten thousand kilograms of total grain outputs newly.This is to guarantee that grain aggregate does not subtract, and realizes the effective way of grain security.
(3) ecological benefits
Plant growth regulator of the present invention is to adopt biochemical synthetic method and add natural drug (containing alkaloid compositions such as sterilization, desinsection) to develop.Analyze through relevant departments' quality inspection, nuisanceless material and toxin composition, noresidue, pollution-free, be ecological, environmental protective class fertilizer, the medicine dual-purpose type conditioning agent that meets national environmental standard fully and produce the non-polluted farm product requirement, its wide popularization and application, very favourable to environmental and ecological environment, have significant ecological benefits.
Five, discuss and build
(1) test, demonstration fully prove: plant growth regulator of the present invention is used on corn, has significant effect of increasing production, 30～133.5 kilograms of the flat volume increase of mu, amplification 11.54～38.36%.The main cause of volume increase is: effectively promoted nourishing and growing of corn seedling stage, guaranteed that the normal differentiation of bud and the hero of taking out of middle and later periods weave silk, helped improving the fertilization ripening rate, realized increasing the grain weightening finish and increase production.
(2) since this year the county influenced by natural calamities such as high temperature rarely seen in the history, dry season, regional area (mainly be hills and along the mountain one band) corn is disaster-stricken serious, output is lower, and situation about being reflected can not be represented the long-term level of production, also needs the coming year carry out test, demonstration in nearly step.
(3) except that the experiment and demonstration of on paddy rice, wheat, corn three generalized grain crops, having carried out plant growth regulator of the present invention and, suggestion also should be carried out test, demonstration on economic crops such as rape, vegetables, fruit tree, edible mushroom, traditional Chinese medicine, flowers, extensively explore application effect and application process on Different Crop, for large scale application provides scientific basis.
Claims (4)
1. a plant growth regulators is characterized in that: comprise the following weight proportion raw material:
Copper sulphate 1-20
Zinc sulphate 1-50
Manganese sulphate 1-30
Iron sulfate 1-40
Sodium molybdate 1-25
Amino acid 0.1-1
Nucleic acid-protein 1-20
Vitamin E 0.001-0.002
Agricultural rare-earth 2-6
Silicic acid 1-30
Sugar 5-20
Water 1000-2000;
Contain the potato PPO compound of 0.5 weight portion, the rhodotorula glutinis L-phenylalnine ammonialyase compound of 0.5 weight portion, the earthworm superoxide dismutase of 0.5 weight portion, the seaweed bio active substance of 0.5 weight portion, the disease-resistant worm compound of 0.5 weight portion in the described conditioning agent of per 100 weight portions;
Described seaweed bio active substance is to be obtained by following extraction step:
A, get 1 kilogram of clean marine alga, add 1 kilogram of wash clean earthworm, add one piece of raw egg and carry out homogenized, obtain mixed serum;
B, by per 1 kilogram of mixed serum add sucrose 40 grams, dusty yeast 20 grams, ammonium sulfate 1.0 grams, 1000 gram water stir the back, be warmed up to 40 ℃, fermentation process obtains zymotic fluid;
C, the 3mol/L phosphate buffer that adds 5 times of volumes in described zymotic fluid mix, and are heated to 100 ℃ of insulations 20-48 hour, obtain mixed solution;
D, the mixed solution temperature is cooled to normal temperature, the pH value of adjusting mixed solution with ammoniacal liquor reaches between the 6-7 then, and acquisition seaweed bio active substance extract is preserved under-4 ℃ of conditions;
Described disease-resistant worm compound is to be obtained by following extraction step:
A, balsam pear, ginger, garlic, rape flower, ginkgo skin, tree-of-heaven, fish Teng, green peel of walnut, castor bean leaf, bunge pricklyash leaf, tarragon are cleaned up with clear water, dry then;
B, above-mentioned raw materials is prepared burden by weight, mix then to pulverize and form crushed material, wherein, 10 parts in balsam pear, 3 parts in ginger, 7 parts in garlic, 10 parts of rape flowers, 10 parts of ginkgo skins, 10 parts of tree-of-heavens, 10 parts of fish Tengs, 10 parts of green peel of walnut, 10 parts of castor bean leaves, 10 parts of bunge pricklyash leafs, 10 parts of tarragons;
C, with the water that crushed material adds 5 times of parts by weight, add 1 ‰ phosphoric acid again, under 100 ℃ of conditions boiling 0.5-1 hour, soak then and placed 5-8 hour, obtain disease-resistant worm compound soup;
D, enantiopathy worm compound soup use the multilayer filtered through gauze, filtering liquid medicine is carried out centrifugation handle, and collect supernatant;
E, the pH value of using ammoniacal liquor to adjust supernatant reach 6.5-7, obtain disease-resistant worm compound, and disease-resistant worm compound is preserved under-4 ℃ of conditions.
2. plant growth regulator according to claim 1 is characterized in that: comprise the following weight proportion raw material:
Copper sulphate 10
Zinc sulphate 25
Manganese sulphate 15
Iron sulfate 20
Sodium molybdate 12
Amino acid 0.5
Nucleic acid-protein 10
Vitamin E 0.0015
Agricultural rare-earth 4
Silicic acid 20
Sugar 12
Water 1500.
3. plant growth regulator according to claim 1 is characterized in that: comprise the following weight proportion raw material:
Copper sulphate 20
Zinc sulphate 50
Manganese sulphate 30
Iron sulfate 40
Sodium molybdate 25
Amino acid/11
Nucleic acid-protein 20
Vitamin E 0.002
Agricultural rare-earth 6
Silicic acid 30
Sugar 20
Water 2000.
4. the production method of a plant growth regulators, it is characterized in that, comprise the following weight proportion raw material, copper sulphate 1-20, zinc sulphate 1-50, manganese sulphate 1-30, iron sulfate 1-40, sodium molybdate 1-25, amino acid 0.1-1, nucleic acid-protein 1-20, vitamin E 0.001-0.002, agricultural rare-earth 2-6, silicic acid 1-30, sugared 5-20, water 1000-2000; Contain the potato PPO compound of 0.5 weight portion, the rhodotorula glutinis L-phenylalnine ammonialyase compound of 0.5 weight portion, the earthworm superoxide dismutase of 0.5 weight portion, the seaweed bio active substance of 0.5 weight portion, the disease-resistant worm compound of 0.5 weight portion in the described conditioning agent of per 100 weight portions;
The extraction step of I, rhodotorula glutinis L-phenylalnine ammonialyase compound:
A, preparation medium comprise following weight percentages in the medium: sucrose 1%, corn steep liquor 3%, earthworm slurry 2%, sodium chloride (NaCL) 0.5%, L-tyrosine 0.1%, dipotassium hydrogen phosphate (K
2HPO
4) 0.1%, defoamer 0.01%, all the other are clean waters, regulate the pH value 5.0～5.5 of medium;
B, medium is carried out sterilization processing, under 30 ℃ of temperature, inoculate bacterial classification then, under 30 ℃ of temperature, cultivated 12-15 hour, under 40 ℃ of temperature, cultivated 5-7 hour, generate zymotic fluid;
C, zymotic fluid is carried out centrifugal treating, collect wet cell;
D, working concentration are that 0.9% physiological saline carries out carrying out washing treatment to wet cell, add 1 gram wet cell in every ml physiological saline, then physiological saline are carried out centrifugal treating, collect wet cell;
E, wet cell and buffer solution are mixed into cell suspending liquid, add 1 gram wet cell in per 4 milliliters of buffer solutions; In cell suspending liquid, add concentration then again and be 10% triton x-100 osmotic treated; The concentration of described buffer solution is 50mmol/L, pH value 8.8;
F, use processor for ultrasonic wave are handled described cell suspending liquid ultrasonic disruption, produce broken liquid;
G, to described broken liquid multilayer filtered through gauze, produce filtered fluid, filtered fluid is carried out centrifugal treating, get supernatant;
H, select molecular cut off 20,000 PS milipore filters to carry out ultrafiltration and concentration to supernatant to handle, obtain once concentration liquid;
The degree of saturation scale of I, the actual volume according to once concentration liquid, temperature and ammonium sulfate calculates and reaches 38% degree of saturation and should add ammonium sulfate consumption in the concentrate, prepares out solid sulphuric acid iron;
J, once concentration liquid is directly poured in the beaker, added solid sulphuric acid iron, then once concentration liquid is carried out refrigerated centrifuge and handle, obtain the secondary supernatant;
K, to secondary supernatant ultrafiltration and concentration, obtain secondary concentration liquid;
The degree of saturation scale of L, the actual volume according to secondary concentration liquid, temperature and ammonium sulfate calculates from the required ammonium sulfate consumption of 38%-75% degree of saturation, prepares out solid sulphuric acid iron;
M, secondary concentration liquid is directly poured in the beaker, added solid sulphuric acid iron, then secondary concentration liquid is carried out refrigerated centrifuge and handle, abandoning supernatant keeps sediment, obtains rhodotorula glutinis L-phenylalnine ammonialyase compound;
N, with the freeze-drying of rhodotorula glutinis L-phenylalnine ammonialyase compound, preserve down at-20 ℃ then;
II, potato PPO (PPO) compound extraction step:
A, with potato clean, section, carry out homogenized then; In the homogenized process, add the 0.03M phosphate buffer of 1 liter of parts by volume in the potato pulp of per 1 kilogram-mass part, mix, obtain potato pulp; The temperature of described 0.03M phosphate buffer is 1 ℃, pH value 5.5;
After B, the homogenized, use the multilayer gauze that potato pulp is filtered, then filtered fluid is carried out centrifugation and handle, obtain the polyphenol oxidase zyme extract;
C, in the polyphenol oxidase zyme extract, add solid ammonium sulfate and make it reach 40% degree of saturation, carry out centrifugation then and handle, obtain supernatant;
D, in supernatant, add solid ammonium sulfate and make it reach 70% degree of saturation, carry out centrifugation then and handle, collect thick enzyme sediment, obtain the potato PPO compound;
The extraction step of III, earthworm superoxide dismutase:
A, the earthworm that will live place light salt brine to clean, and the content of salt is no more than 0.3% in the light salt brine; The earthworm that will live is rubbed processing, and in the rubbing process, the lactose that adds about 0.5 vitamin C that restrains and 0.5 gram in per 1000 gram earthworms obtains earthworm juice as protectant;
B, earthworm juice is obtained earthworm freeze after freezing processing under-4 ℃～-1 ℃ the temperature;
C, earthworm frozen put into the phosphate buffer homogenized, the earthworm of per 1 kilogram-mass part is frozen the phosphate buffer of 3 liters of parts by volume of configuration, and the parameter of phosphate buffer is 0.05mol/L, pH value 7.8, and homogenized finishes, carry out centrifugation and handle, obtain one time supernatant;
D, supernatant is used the multilayer filtered through gauze, then filtered fluid is carried out centrifugation and handle, obtain the secondary supernatant;
E, will be in the above-mentioned clear liquid of secondary add the acetone mixed processing of 1.25 times of parts by volume, leave standstill processing after, the collecting precipitation thing;
F, described sediment joined in the phosphate buffer dissolve, obtain lysate; The phosphate buffer of 3 liters of parts by volume of sediment configuration of per 1 kilogram-mass part, the parameter of phosphate buffer is 0.05mol/L, pH value 7.8;
G, to the water-bath heating 20 minutes under 65 ℃ of conditions of described lysate, left standstill 10 minutes, water-bath is heated to 65 ℃ again, be cooled to 4 ℃ then rapidly, lysate is carried out centrifugation handle, obtain three times supernatant, contain the earthworm superoxide dismutase in the supernatant;
H, the earthworm superoxide dismutase is preserved under-4 ℃ of conditions;
IV, seaweed bio active substance extraction step:
A, get 1 kilogram of clean marine alga, add 1 kilogram of wash clean earthworm, add one piece of raw egg and carry out homogenized, obtain mixed serum;
B, by per 1 kilogram of mixed serum add sucrose 40 grams, dusty yeast 20 grams, ammonium sulfate 1.0 grams, 1000 gram water stir the back, be warmed up to 40 ℃, fermentation process obtains zymotic fluid;
C, the 3mol/L phosphate buffer that adds 5 times of volumes in described zymotic fluid mix, and are heated to 100 ℃ of insulations 20-48 hour, obtain mixed solution;
D, the mixed solution temperature is cooled to normal temperature, the pH value of adjusting mixed solution with ammoniacal liquor reaches between the 6-7 then, and acquisition seaweed bio active substance extract is preserved under-4 ℃ of conditions;
V, disease-resistant worm compound extraction step:
A, balsam pear, ginger, garlic, rape flower, ginkgo skin, tree-of-heaven, fish Teng, green peel of walnut, castor bean leaf, bunge pricklyash leaf, tarragon are cleaned up with clear water, dry then;
B, above-mentioned raw materials is prepared burden by weight, mix then to pulverize and form crushed material, wherein, 10 parts in balsam pear, 3 parts in ginger, 7 parts in garlic, 10 parts of rape flowers, 10 parts of ginkgo skins, 10 parts of tree-of-heavens, 10 parts of fish Tengs, 10 parts of green peel of walnut, 10 parts of castor bean leaves, 10 parts of bunge pricklyash leafs, 10 parts of tarragons;
C, with the water that crushed material adds 5 times of parts by weight, add 1 ‰ phosphoric acid again, under 100 ℃ of conditions boiling 0.5-1 hour, soak then and placed 5-8 hour, obtain disease-resistant worm compound soup;
D, enantiopathy worm compound soup use the multilayer filtered through gauze, filtering liquid medicine is carried out centrifugation handle, and collect supernatant;
E, the pH value of using ammoniacal liquor to adjust supernatant reach 6.5-7, obtain disease-resistant worm compound, and disease-resistant worm compound is preserved under-4 ℃ of conditions;
VI, above-mentioned raw materials is got all the ready, mixed according to common process and get final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101641597A CN101406203B (en) | 2007-10-08 | 2007-10-08 | Plant growth regulator and production thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101641597A CN101406203B (en) | 2007-10-08 | 2007-10-08 | Plant growth regulator and production thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101406203A CN101406203A (en) | 2009-04-15 |
| CN101406203B true CN101406203B (en) | 2011-06-01 |
Family
ID=40569591
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007101641597A Expired - Fee Related CN101406203B (en) | 2007-10-08 | 2007-10-08 | Plant growth regulator and production thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101406203B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206103A (en) * | 2010-03-30 | 2011-10-05 | 陈金明 | Active biological nutrient solution, and manufacturing method and purpose thereof |
| CN102326588A (en) * | 2011-06-28 | 2012-01-25 | 蔡明� | Plant growth nutrient solution |
| FR2999386B1 (en) | 2012-12-17 | 2015-04-03 | Bionov | COMPOSITION FOR STIMULATING THE VITALITY OF PLANTS |
| CN104322565A (en) * | 2014-10-21 | 2015-02-04 | 苏州科大微龙信息技术有限公司 | Nano-copper rice blast preventing-treating agent and preparation method thereof |
| CN106083482A (en) * | 2016-06-22 | 2016-11-09 | 沙玉涵 | Plant nature auxin and preparation method thereof |
| CN109362813A (en) * | 2018-11-05 | 2019-02-22 | 贵州好菇粮农业科技有限公司 | A kind of mushroom growth regulator and preparation method thereof |
| CN114931151A (en) * | 2022-05-13 | 2022-08-23 | 深圳市和维斯科技有限公司 | Holographic plant growth promoter and preparation method and use method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1273776A (en) * | 1999-01-27 | 2000-11-22 | 魏宏儒 | Chemical for preventing and treating vascular bundle diseases of plant |
| CN1289538A (en) * | 1999-09-29 | 2001-04-04 | 杨宝详 | Plant growth regulator and preparing process thereof |
-
2007
- 2007-10-08 CN CN2007101641597A patent/CN101406203B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1273776A (en) * | 1999-01-27 | 2000-11-22 | 魏宏儒 | Chemical for preventing and treating vascular bundle diseases of plant |
| CN1289538A (en) * | 1999-09-29 | 2001-04-04 | 杨宝详 | Plant growth regulator and preparing process thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101406203A (en) | 2009-04-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102515952B (en) | Chitosan coated alga bioorganic fertilizer and preparation method thereof | |
| CN102030581B (en) | Novel multi-functional biological organic seed dressing agent and method for preparing same | |
| CN108440146A (en) | A kind of full trophic function type suspension liquid fertilizer of high concentration and preparation method thereof | |
| CN102351592A (en) | High-efficiency multifunctional foliage fertilizer and production method thereof | |
| CN101406203B (en) | Plant growth regulator and production thereof | |
| CN103875491B (en) | A kind of organic scented rice implantation methods | |
| CN111233578A (en) | Composition and method for biologically controlling orobanche coerulescens | |
| CN104130068A (en) | Compound multifunctional biological foliage fertilizer | |
| CN104478565A (en) | Ferment liquid fertilizer and preparation method thereof | |
| CN107624518A (en) | Increase the method for biological organic C storage and carbon sequestration amount | |
| CN110054530A (en) | A kind of compound organic green manure of apple tree and preparation method thereof | |
| CN109485508A (en) | A kind of control method of plant root rot | |
| CN111675555A (en) | Application of anabaena and/or spirulina extract as biostimulant in agricultural production | |
| CN107176865A (en) | The sweetened liquid fertilizer method of preparation and use of marine alga earthworm hydrolyzate efficient bio-active | |
| CN102260116B (en) | Plant nutrient containing oligosaccharide and organic silicon | |
| CN113475293A (en) | Water and fertilizer integrated diversified combined drip irrigation planting method for low-hill forests | |
| CN116199544A (en) | A kind of selenium-enriched active organic fertilizer in saline-alkali land and preparation method thereof | |
| CN111011139A (en) | Planting method of selenium-rich pollution-free potatoes | |
| CN104844285A (en) | Method for preparing bio-organic fertilizer for improving immunity of cherry tomatoes | |
| CN104230404A (en) | Preparation method of enzymatic hydrolysis algae organic fertilizer and product thereof | |
| KR101238338B1 (en) | Fertilizing method of grape having a planty of mineral and the grape thereby | |
| CN111592405B (en) | Organic water-soluble fertilizer capable of resisting wheat dry hot air and powdery mildew as well as preparation and application thereof | |
| CN115873917A (en) | Enzymolysis small molecular peptide composition and preparation method and application thereof | |
| CN114920598A (en) | Biological fertilizer and preparation method and application thereof | |
| CN106699304A (en) | Biological leaf fertilizer and production method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110601 Termination date: 20171008 |