CN1006330B - Method of bod determination - Google Patents
Method of bod determinationInfo
- Publication number
- CN1006330B CN1006330B CN 88100833 CN88100833A CN1006330B CN 1006330 B CN1006330 B CN 1006330B CN 88100833 CN88100833 CN 88100833 CN 88100833 A CN88100833 A CN 88100833A CN 1006330 B CN1006330 B CN 1006330B
- Authority
- CN
- China
- Prior art keywords
- oxygen demand
- water
- biochemical oxygen
- deactivator
- water sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims abstract description 16
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 41
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 41
- 239000001301 oxygen Substances 0.000 claims abstract description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 39
- 238000003556 assay Methods 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 abstract description 8
- 238000012360 testing method Methods 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract 5
- 239000003638 chemical reducing agent Substances 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 abstract 1
- 239000012895 dilution Substances 0.000 description 10
- 238000010790 dilution Methods 0.000 description 10
- 230000036284 oxygen consumption Effects 0.000 description 10
- 230000003647 oxidation Effects 0.000 description 6
- 238000007254 oxidation reaction Methods 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000002351 wastewater Substances 0.000 description 3
- 150000001299 aldehydes Chemical class 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000007655 standard test method Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides a method for measuring biochemical oxygen demand, which belongs to a testing method. The accuracy of the existing method used in general for testing the biochemical oxygen demand is poor, and particularly, when polluted water contains reductants, such as Fe<2+>, Mn<2+>, SO3<2->, S<2->, aldehyde, etc., the existing method measures the sum of biochemical oxygen demand and chemical oxygen demand. The method provided by the present invention changes a mode of twice measurement before and after cultured into a mode of once measurement after cultured, the measurement procedure is simplified, and the accuracy is improved. In addition, the method is favourable for applying an oxygen dissolved measurement instrument to the measurement of the biochemical oxygen demand.
Description
The present invention relates to a kind of method of testing, the method for testing of specifically a kind of water, waste water, it comprises the pre-service of water sample, cultivation, chemical analysis or the instrumental analysis process of water sample.
Biochemical oxygen demand is to weigh sanitary sewage, industrial waste water, polluted surface water, an important indicator of biodegradable organic content.Currently used method is " water and waste water standard test method " the 15th edition disclosed assay method.It comprises the steps: sample is contained in the airtight bottle well, under defined terms this bottle is cultivated a period of time of stipulating, reaches to cultivate before beginning cultivation to finish mensuration dissolved oxygen DO later on, calculates biochemical oxygen demand from the difference of that begin and last dissolved oxygen DO.The existing weak point of this method is: 1. contain just like Fe in sewage
2+, Mn
2+, SO
2- 3, S
2-, during reductive agent such as aldehydes, in incubation, these materials also can with the dissolved oxygen DO reaction in the water, and produce the chemical oxidation oxygen consumption, with this method measured be biochemical oxygen consumption and chemical oxidation oxygen consumption sum, rather than biochemical oxygen demand really.2. respectively measure dissolved oxygen DO one time before and after cultivating, twice mensuration of different time easily causes error, and when especially measuring dissolved oxygen DO with dissolved oxygen meter, error is bigger.
The object of the present invention is to provide an a kind of assay method of biochemical oxygen demand, reduce error at measurment.
The object of the present invention is achieved like this: take two parts of the good water samples of seeding-dilution water or dilution water pre-service.Portion wherein adds Hg
2+As deactivator, deactivator can also be H
+Another part does not add deactivator.Two parts of water samples are put into incubator simultaneously, and after a period of time of cultivation regulation, the dissolved oxygen DO with dissolved oxygen meter or two parts of water samples of iodometric determination calculates biochemical oxygen demand.
Method of the present invention compared with prior art has following advantage:
1, in being added with the water sample of deactivator, inactivation of bacteria does not produce biological oxygen consumption, but Fe
2+, Mn
2+, SO
2- 3, S
2-, reductive agent such as aldehydes still produces the chemical oxidation oxygen consumption, therefore by the measured dissolved oxygen DO of the water sample that is added with deactivator, the dissolved oxygen DO when having the chemical oxidation oxygen consumption not have biochemical oxygen consumption.And what recorded by the water sample that does not add deactivator is that chemical oxidation oxygen consumption, dissolved oxygen DO when biochemical oxygen consumption is arranged are promptly arranged.When with being added with the poor of deactivator and two parts of water sample dissolved oxygen DOs that do not add deactivator after cultivating, when calculating biochemical oxygen demand, can eliminate the chemical oxidation oxygen consumption, record real biochemical oxygen demand.
2, measure dissolved oxygen DO twice with cultivating the back before will cultivating, once measure after making into to cultivate, but lowering apparatus, reagent error helps dissolved oxygen meter is introduced in the mensuration of biochemical oxygen demand.
3, simplified operation.
Method of the present invention can realize by following embodiment:
Water sample dilutes with seeding-dilution water or dilution water.Good water sample is divided into two parts to get pre-service, adds HgCL in portion wherein
2As deactivator, HgCL
2Addition be 2.6～20mg/L.Also can do deactivator, with HCL the pH value of water sample be transferred to below 2.1 and get final product with HCL.Two parts of water samples are put into incubator simultaneously, cultivated five down at 20 ℃ ± 1 ℃.Take out the dissolved oxygen DO of water sample nutrient solution with dissolved oxygen meter or two parts of water samples of iodometric determination.Be calculated as follows out five-day BOD.
BOD（mg/L）＝〔（D
1-D
2）-（B
1-B
2）f〕 1/(p)
In the formula: BOD is a biochemical oxygen demand
D
1Dissolved oxygen DO (mg/L) for the water sample nutrient solution that contains deactivator
D
2Dissolved oxygen DO (mg/L) for the water sample nutrient solution that do not contain deactivator.
B
1Be the seeding-dilution water that contains deactivator or the dissolved oxygen DO (mg/L) of dilution water.
B
2Be the seeding-dilution water that do not contain deactivator or the dissolved oxygen DO (mg/L) of dilution water.
F is seeding-dilution water or dilution water proportion in the water sample nutrient solution.
P is waste water proportion in the water sample nutrient solution.
Claims (4)
1, a kind of assay method of biochemical oxygen demand, it comprises the processing of giving of water sample, the cultivation of water sample, chemistry or instrumental analysis process, it is characterized in that: the water sample that portion is added deactivator was cultivated 5 days simultaneously with a water sample that does not add deactivator, measure the dissolved oxygen DO of two parts of water samples simultaneously, and calculate biochemical oxygen demand, its computing formula is:
BOD(mg/l)=[(D
1-D
2)-(B
1-B
2)f] 1/(P)
2, the assay method of a kind of biochemical oxygen demand according to claim 1 is characterized in that: described deactivator is Hg
2+Or H
+
3, the assay method of a kind of biochemical oxygen demand according to claim 2 is characterized in that: Hg
2+(with HgCL
2Meter) consumption is 2.6～20mg/L.
4, the assay method of a kind of biochemical oxygen demand according to claim 2 is characterized in that: use H
+(adding with HCL), water transfer sample pH value was less than 2.1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 88100833 CN1006330B (en) | 1988-02-08 | 1988-02-08 | Method of bod determination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 88100833 CN1006330B (en) | 1988-02-08 | 1988-02-08 | Method of bod determination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN88100833A CN88100833A (en) | 1988-08-24 |
| CN1006330B true CN1006330B (en) | 1990-01-03 |
Family
ID=4831522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 88100833 Expired CN1006330B (en) | 1988-02-08 | 1988-02-08 | Method of bod determination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1006330B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102353417A (en) * | 2011-08-26 | 2012-02-15 | 河南师范大学 | Method for directly measuring quantity of biodegraded organic matter of biological active carbon |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034444C (en) * | 1994-01-21 | 1997-04-02 | 上海市环境保护科学研究院 | Method and device for continuously measuring chemical oxygen demand |
| CN114047228B (en) * | 2021-11-09 | 2023-08-04 | 同济大学 | Device and method for analyzing oxygen-consuming pollutants of sediment |
-
1988
- 1988-02-08 CN CN 88100833 patent/CN1006330B/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102353417A (en) * | 2011-08-26 | 2012-02-15 | 河南师范大学 | Method for directly measuring quantity of biodegraded organic matter of biological active carbon |
Also Published As
| Publication number | Publication date |
|---|---|
| CN88100833A (en) | 1988-08-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C13 | Decision | ||
| GR02 | Examined patent application | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |