CN109805009A - A kind of chlorine-containing disinfectant and preparation method thereof - Google Patents
A kind of chlorine-containing disinfectant and preparation method thereof Download PDFInfo
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- CN109805009A CN109805009A CN201910069561.XA CN201910069561A CN109805009A CN 109805009 A CN109805009 A CN 109805009A CN 201910069561 A CN201910069561 A CN 201910069561A CN 109805009 A CN109805009 A CN 109805009A
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- chlorine
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- containing disinfectant
- sodium
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- 239000000460 chlorine Substances 0.000 title claims abstract description 61
- 229910052801 chlorine Inorganic materials 0.000 title claims abstract description 61
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 239000000645 desinfectant Substances 0.000 title claims abstract description 53
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000005708 Sodium hypochlorite Substances 0.000 claims abstract description 17
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 17
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 13
- 239000011734 sodium Substances 0.000 claims abstract description 13
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 13
- -1 tri methylene phosphonic acid Chemical compound 0.000 claims abstract description 12
- WBIQQQGBSDOWNP-UHFFFAOYSA-N 2-dodecylbenzenesulfonic acid Chemical compound CCCCCCCCCCCCC1=CC=CC=C1S(O)(=O)=O WBIQQQGBSDOWNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 125000002648 azanetriyl group Chemical group *N(*)* 0.000 claims abstract description 11
- 229940060296 dodecylbenzenesulfonic acid Drugs 0.000 claims abstract description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims abstract description 10
- 239000008367 deionised water Substances 0.000 claims abstract description 10
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 10
- 239000001103 potassium chloride Substances 0.000 claims abstract description 10
- 235000011164 potassium chloride Nutrition 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 claims abstract description 8
- 238000004062 sedimentation Methods 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 3
- 238000001914 filtration Methods 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 2
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- 230000001954 sterilising effect Effects 0.000 abstract description 20
- 230000002147 killing effect Effects 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 abstract description 6
- 238000007599 discharging Methods 0.000 abstract description 3
- 230000003647 oxidation Effects 0.000 abstract description 2
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 28
- 230000000052 comparative effect Effects 0.000 description 25
- 238000012360 testing method Methods 0.000 description 14
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 14
- 229940033663 thimerosal Drugs 0.000 description 14
- 238000004659 sterilization and disinfection Methods 0.000 description 8
- 239000004744 fabric Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000003641 microbiacidal effect Effects 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 230000000249 desinfective effect Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 235000014347 soups Nutrition 0.000 description 3
- VPTUPAVOBUEXMZ-UHFFFAOYSA-N (1-hydroxy-2-phosphonoethyl)phosphonic acid Chemical compound OP(=O)(O)C(O)CP(O)(O)=O VPTUPAVOBUEXMZ-UHFFFAOYSA-N 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- SNRUBQQJIBEYMU-UHFFFAOYSA-N dodecane Chemical compound CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- SSILHZFTFWOUJR-UHFFFAOYSA-N hexadecane-1-sulfonic acid Chemical compound CCCCCCCCCCCCCCCCS(O)(=O)=O SSILHZFTFWOUJR-UHFFFAOYSA-N 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229910001950 potassium oxide Inorganic materials 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- SRSXLGNVWSONIS-UHFFFAOYSA-N benzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-N 0.000 description 1
- 229940092714 benzenesulfonic acid Drugs 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 125000001309 chloro group Chemical group Cl* 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 239000001230 potassium iodate Substances 0.000 description 1
- JLKDVMWYMMLWTI-UHFFFAOYSA-M potassium iodate Chemical compound [K+].[O-]I(=O)=O JLKDVMWYMMLWTI-UHFFFAOYSA-M 0.000 description 1
- 235000006666 potassium iodate Nutrition 0.000 description 1
- 229940093930 potassium iodate Drugs 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- HQAITFAUVZBHNB-UHFFFAOYSA-N sodium;pentahydrate Chemical compound O.O.O.O.O.[Na] HQAITFAUVZBHNB-UHFFFAOYSA-N 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 239000002435 venom Substances 0.000 description 1
- 210000001048 venom Anatomy 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of chlorine-containing disinfectant and preparation method thereof, raw material includes that (methylene) the tri methylene phosphonic acid N- of nitrilo three in terms of mass fraction aoxidizes sylvite 1-5 parts, 0.1-1.0 parts of sodium cetanesulfonate, 0.01-1.0 parts of dodecyl benzene sulfonic acid, 0.001-0.5 parts of sodium hydroxide, 40-70 parts of sodium hypochlorite, 22.5-58.889 parts of deionized water；A kind of preparation method of chlorine-containing disinfectant comprises the technical steps that: (methylene) the tri methylene phosphonic acid N- of nitrilo three oxidation sylvite, sodium cetanesulfonate, dodecyl benzene sulfonic acid and sodium hydroxide being added into deionized water, is uniformly mixed；Sodium hypochlorite is added, is uniformly mixed；Chlorine-containing disinfectant is filtered to obtain after standing sedimentation.The present invention not only ensure that the stability and efficient sterilizing effect of chlorine-containing disinfectant, but also be applicable to various condition of water quality in application, is conducive to discharging rapidly for effective chlorine composition, plays the role of quickly killing microorganism.
Description
Technical field
The present invention relates to disinfectant preparation technical fields, more specifically, it is related to a kind of chlorine-containing disinfectant and its preparation
Method.
Background technique
Chlorine-containing disinfectant Yin Qiyi is diffused into bacterium surface, and various microorganisms can be killed in the short time, is that one kind efficiently disappears
Toxic agent is widely used in the disinfecting of textile, hotel, hospital, food-processing industry, family etc..But chlorine-containing disinfectant
In effective component be sodium hypochlorite, since sodium hypochlorite property is unstable, vulnerable to the influence of light, heat and humidity, losing it has
Imitate ingredient.Sodium hypochlorite is dissolved in unstable after water, decomposes and generates chlorine, not only pollutes environment, but also bactericidal effect is significantly
Discount.
Summary of the invention
In view of the deficiencies of the prior art, the present invention intends to provide a kind of chlorine-containing disinfectant, not only guarantee
Stability and the efficient sterilizing effect of chlorine-containing disinfectant, and the present invention is applicable to various condition of water quality in application, has
Conducive to discharging rapidly for effective chlorine composition, play the role of quickly killing microorganism.
Above-mentioned technical purpose of the invention has the technical scheme that
A kind of chlorine-containing disinfectant, in terms of mass fraction, raw material includes (methylene) the tri methylene phosphonic acid N- of nitrilo three oxidation sylvite 1-5
Part, 0.1-1.0 parts of sodium cetanesulfonate, 0.01-1.0 parts of dodecyl benzene sulfonic acid, 0.001-0.5 parts of sodium hydroxide, secondary chlorine
Sour sodium 40-70 parts, 22.5-58.889 parts of deionized water.
Further, the pH value of the chlorine-containing disinfectant controls between 8.0-14.0.
The purpose of the present invention is to provide a kind of chlorine-containing disinfectants and preparation method thereof, have.The advantages of.
Above-mentioned technical purpose of the invention has the technical scheme that
A kind of preparation method of chlorine-containing disinfectant, comprises the technical steps that:
(1) (methylene) the tri methylene phosphonic acid N- of nitrilo three is added into deionized water and aoxidizes sylvite, sodium cetanesulfonate, dodecyl
Benzene sulfonic acid and sodium hydroxide are uniformly mixed；
(2) sodium hypochlorite is added into mixture obtained by step (1), is uniformly mixed；
(3) chlorine-containing disinfectant is filtered to obtain after standing sedimentation.
Further, in terms of mass fraction, (methylene) the tri methylene phosphonic acid N- of nitrilo three aoxidizes sylvite 1-5 parts, cetyl sulfonic acid
0.1-1.0 parts of sodium, 0.01-1.0 parts of dodecyl benzene sulfonic acid, 0.001-0.5 parts of sodium hydroxide, 40-70 parts of sodium hypochlorite, go from
Sub- water 22.5-58.889 parts.
Further, the pH value of the chlorine-containing disinfectant is controlled in 8.0-14.0.
Further, the standing sedimentation time is 20-30 hours in step (3).
Further, filtering uses 200 mesh filter clothes in step (3).
In conclusion the invention has the following advantages:
The first, the present invention is by aoxidizing sylvite and sodium cetanesulfonate and dodecane for (methylene) the tri methylene phosphonic acid N- of nitrilo three
Base benzene sulfonic acid compounding, is in three in same solution, so that aqueous sodium hypochlorite solution is in chemical reaction dynamic balancing state,
It ensure that the stability and efficient sterilizing effect of chlorine-containing disinfectant of the present invention.
The second, the present invention is applicable to various condition of water quality in application, is conducive to discharging rapidly for effective chlorine composition, rises
To the quick effect for killing microorganism.
Specific embodiment
Invention is further described in detail with reference to embodiments.
Embodiment 1
The preparation of chlorine-containing disinfectant: (1) it into deionized water 22.5Kg is added nitrilo three (methylene) tri methylene phosphonic acid N- and aoxidizes sylvite
5Kg, sodium cetanesulfonate 1.0Kg, dodecyl benzene sulfonic acid 1.0Kg and sodium hydroxide 0.5Kg are uniformly mixed；(2) to step
(1) sodium hypochlorite 70Kg is added in gained mixture, is uniformly mixed, is cleaned to obtain chlorine-containing disinfectant with 200 mesh filter-cloth filterings；(3)
Chlorine-containing disinfectant is obtained using 200 mesh filter-cloth filterings after standing sedimentation 30 hours.
Embodiment 2
The preparation of chlorine-containing disinfectant: (1) nitrilo three (methylene) tri methylene phosphonic acid N- potassium oxide into deionized water 40.6945Kg is added
Salt 3Kg, cetyl sulfonic acid 0.55Kg, dodecyl benzene sulfonic acid 0.505Kg and sodium hydroxide 0.2505Kg are uniformly mixed；(2)
Sodium hypochlorite 55Kg is added into mixture obtained by step (1), is uniformly mixed, is cleaned to obtain chlorine-containing disinfecting with 200 mesh filter-cloth filterings
Agent；(3) chlorine-containing disinfectant is obtained using 200 mesh filter-cloth filterings after standing sedimentation 24 hours.
Embodiment 3
The preparation of chlorine-containing disinfectant: (1) nitrilo three (methylene) tri methylene phosphonic acid N- potassium oxide into deionized water 58.889Kg is added
Salt 1Kg, sodium cetanesulfonate 0.1Kg, dodecyl benzene sulfonic acid 0.01Kg and sodium hydroxide 0.001Kg are uniformly mixed；(2)
Sodium hypochlorite 40Kg is added into mixture obtained by step (1), is uniformly mixed, is cleaned to obtain chlorine-containing disinfecting with 200 mesh filter-cloth filterings
Agent；(3) chlorine-containing disinfectant is obtained using 200 mesh filter-cloth filterings after standing sedimentation 20 hours.
Comparative example 1
It is on March 25th, 2009 as publication date, a kind of thimerosal disclosed in the patent document of Publication No. CN101390522A.It is right
The thimerosal that ratio 1 provides specifically: 84 thimerosal 500g, Potassiumiodate 3.333g, fatty alcohol containing effective chlorine >=5%
0.625g, polyvinylpyrrolidone 0.625g, sodium carbonate 2.0g.
Comparative example 2
It is on March 8th, 2017 as data of publication of application, application publication number is that one kind disclosed in the patent document of CN106472562A is steady
It is formed 84 thimerosals and preparation method thereof.84 thimerosal of stable type that comparative example 2 provides specifically: first match water 59.5Kg addition
In batch can, stirring is opened, sodium hydroxide 2Kg, HEDP (hydroxy ethylene diphosphonic acid) 1.5Kg, sodium cetanesulfonate
Material-compound tank is added in 3.0Kg, sodium bromide 1.2Kg, EDTA-4Na 0.8Kg, and ingredient is added in sodium hypochlorite 32Kg after mixing evenly
Tank settles 24 hours after mixing evenly and filters, obtains 84 thimerosal of stable type.
Comparative example 3
As data of publication of application be on December 19th, 2017, application publication number is one kind disclosed in the patent document of CN107484771A
84 thimerosals and preparation method thereof.84 thimerosals that comparative example 3 provides specifically: by 0.61Kg metasilicate pentahydrate sodium, 0.123Kg
In tertiary sodium phosphate, 0.509Kg sodium hypochlorite merging reactor, 1.829Kg water is added, stirring keeps solids completely molten in 30 minutes
3.019Kg water is added in solution into reactor again, stirs 21 minutes, it is spare to obtain mixed solution A；By 3.97Kg sodium hydroxide,
9.969Kg sodium hypochlorite, mixed solution A are sequentially added in 79.804Kg and are mixed, and after stirring 32 minutes, are placed 2.2 hours, are obtained
84 thimerosals.
Chlorine-containing disinfectant and the thimerosal of the offer of comparative example 1, comparative example 2 for embodiment 1-3 preparation provide steady
84 thimerosals that 84 thimerosals, comparative example 3 provide of being formed carry out the test of toxicity biological assessment, and the present invention chooses Escherichia coli
Bacteriostatic experiment is carried out with staphylococcus aureus.
Broth medium: 10.00g peptone, 5.00g beef extract and 10.00g sodium chloride are weighed, is dissolved in
In 1000mL distilled water, tepor dissolution adjusts pH to alkalescent, boils, adjusting pH makes after sterilizing to be 7.2~7.4, through 121 DEG C
It is spare after pressuresteam sterilization 30 minutes.
NA culture medium: weighing 10.00g peptone, 5.00g beef extract and 10.00g sodium chloride, is dissolved in 1000mL distillation
In water, tepor dissolution adjusts pH to 7.2~7.4, dissolves by heating after agar is added, and filtering packing is gone out through 121 DEG C of steams
It is spare after bacterium 30 minutes.
The configuration of dilution: 1.00g peptone, being dissolved in 100mL concentration is to adjust in 0.03mo1/L phosphate buffer
Save pH to 7.2~7.4, through 121 DEG C after pressuresteam sterilization 30 minutes it is spare.
The configuration of neutralizer: weighing 1.00g sodium thiosulfate, and being dissolved in 100mL concentration is 0.03mo1/L phosphate-buffered
In liquid, through 121 DEG C after pressuresteam sterilization 15 minutes it is spare.
Sterilizing experimental method: according to the evaluation method of National Standard of the People's Republic of China-disinfection and sterilization effect
Implement with standard (GB159981-1995).
Quantitative sterilizing test: after quantitative sterilizing test measurement is acted on by chlorine-containing disinfectant, sample surviving microorganisms quantity
Test method indicates result with sterilizing rate.For the evaluation to chlorine-containing disinfectant killing effect.At 19~21 DEG C, by bacterium solution
Carry out count plate, and the bacteria suspension for being 5 × 105 and 5 × 106cfu/mL at bacteria containing amount with diluent preparing.By sterilizing test tubes 8
Branch is arranged on rack for test tube, label pipe number.Chlorine-containing disinfectant, reality prepared by the embodiment 1 of 2.5mL sequentially is added to sterilizing test tubes
Thimerosal, the offer of comparative example 2 that the chlorine-containing disinfectant of the preparation of example 2, chlorine-containing disinfectant prepared by embodiment 3, comparative example 1 provide are provided
84 thimerosal of stable type, comparative example 3 provide 84 thimerosals and water.Adding 0.5mL bacteria suspension, (bacteria containing amount is 5 × 105~5
× 106cfu/m L) in each test tube, it mixes and starts to clock.Respectively at 4 different intervals (2min, 5min, 10min
And 20min), it respectively takes 0.5mL bacterium medicine mixed liquor to move into 4.5mL neutralizer and mixes.0.2mL is taken to be inoculated in after neutralization 10min flat
(10,100 or 1000 times of three concentration dilutions are carried out to its bacterium medicine mixed liquor) on ware and carries out count plate.Each sample repeats 3
It is secondary.The dilution of equivalent is used in control, while being handled according to the method described above.Extrapolate each sample survival bacterium number (cfu/
mL)。
Killing rate (%)=(control group survival bacterium number-test group survival bacterium number)/control group survival bacterium number × 100%
Result judgement: testing killing rate >=99.9% of chlorine-containing disinfectant, and it is qualified to be judged to disinfection.
The chlorine-containing disinfectant of embodiment 1-3 preparation and thimerosal, the comparison of the offer of comparative example 1 are provided according to the above method
84 thimerosals of 84 thimerosal of stable type, the offer of comparative example 3 that example 2 provides the results are shown in Table 1 to the killing rate for trying bacterium.
Table 1
As it can be seen from table 1 passing through comparative example 1-3 and comparative example 1-3 it is found that embodiment 1-3 and comparative example 1 have efficiently
Bactericidal effect.Thus, chlorine-containing disinfectant provided by the invention has efficient sterilizing effect.
Qualitative disinfection experiment: at 19~21 DEG C, bacterium solution is subjected to count plate, and be at bacteria containing amount with diluent preparing
The bacteria suspension of 5 × 105 and 5 × 106cfu/mL.Sterilizing test tubes 8 are arranged on rack for test tube, label pipe number.Sequentially to sterilizing
Test tube is added the chlorine-containing disinfectant of the preparation of embodiment 1 of 2.5mL, chlorine-containing disinfectant prepared by embodiment 2, embodiment 3 and prepares
The 84 of 84 thimerosal of stable type, the offer of comparative example 3 that chlorine-containing disinfectant, the thimerosal of the offer of comparative example 1, comparative example 2 provide disappear
Venom and water.Add bacteria suspension 2.5mL in each test tube, mix and record each pipe and add the bacterium time, makes in bacterium medicine mixed liquor containing bacterium
Amount is 105~106cfu/mL.Each pipe takes out 0.5mL after adding bacterium to act on 10min, is added in 4.5mL neutralizer, in
After 10min, takes out 0.5mL and be added in 4.5mL nutrient broth.It is small that the broth tubes of inoculated bacteria are placed on 37 DEG C of cultures 48
When, PRELIMINARY RESULTS is observed, asepsis growth pipe continued culture to the 7th day.Experiment is repeated 3 times.
If meat soup is muddy, then it represents that have bacterium growth, be denoted as the positive；If culture was to the 7th day, meat soup is clarified, then it represents that sterile
Growth, is denoted as feminine gender.Slight haziness is indicated with (+), and (++) indicates that severe is muddy, and (-) indicates clarification.To the meat for being difficult to determine
Soup takes 0.1mL to be inoculated in nutrient agar panel and is smoothened with sterilizing spreading rod, is put in 37 DEG C of culture 48h, observes colonial morphology, and
Smear staining microscopy is done, indicator bacteria growth is judged whether there is, has indicator bacteria growth to be denoted as the positive.3 tests are raw without indicator bacteria
It is long to indicate to reach sterilization effect.
The chlorine-containing disinfectant of embodiment 1-3 preparation and thimerosal, the comparison of the offer of comparative example 1 are provided according to the above method
84 thimerosals of 84 thimerosal of stable type, the offer of comparative example 3 that example 2 provides the results are shown in Table 2 to the inhibitory activity for trying bacterium.
Table 2
From table 2 it can be seen that the mean microbicidal effect of embodiment 1-3 and comparative example 1 was suitable by 48 hours action time,
And it is substantially better than the mean microbicidal effect of comparative example 2 and comparative example 3.But by 168 hours action time, embodiment 1-3
Mean microbicidal effect be substantially better than the mean microbicidal effect of comparative example 1.It follows that chlorine-containing disinfectant tool provided by the invention
There is efficient sterilizing effect, even across 7 days time, chlorine-containing disinfectant of the invention still had preferable bactericidal effect, explanation
The stability of chlorine-containing disinfectant provided by the invention.
In addition, chlorine-containing disinfectant provided by the invention is applicable to various condition of water quality in application, be conducive to effective chlorine
Ingredient discharges rapidly, and plays the role of quickly killing microorganism.
It should be understood that preparation method described in the embodiment of the present invention is only used for illustrating the present invention, rather than to this
The limitation of invention belongs to the simple modifications of preparation method of the present invention under concept thereof of the invention claimed
Range.
Claims (7)
1. a kind of chlorine-containing disinfectant, which is characterized in that in terms of mass fraction, raw material includes (methylene) tri methylene phosphonic acid of nitrilo three N-
Aoxidize sylvite 1-5 parts, 0.1-1.0 parts of sodium cetanesulfonate, 0.01-1.0 parts of dodecyl benzene sulfonic acid, sodium hydroxide 0.001-
0.5 part, 40-70 parts of sodium hypochlorite, 22.5-58.889 parts of deionized water.
2. a kind of chlorine-containing disinfectant according to claim 1, which is characterized in that the pH value control of the chlorine-containing disinfectant exists
Between 8.0-14.0.
3. a kind of preparation method of chlorine-containing disinfectant, which is characterized in that comprise the technical steps that:
(1) (methylene) the tri methylene phosphonic acid N- of nitrilo three is added into deionized water and aoxidizes sylvite, sodium cetanesulfonate, dodecyl
Benzene sulfonic acid and sodium hydroxide are uniformly mixed；
(2) sodium hypochlorite is added into mixture obtained by step (1), is uniformly mixed；
(3) chlorine-containing disinfectant is filtered to obtain after standing sedimentation.
4. a kind of preparation method of chlorine-containing disinfectant according to claim 3, which is characterized in that in terms of mass fraction, nitrogen
(methylene) the tri methylene phosphonic acid N- of river three aoxidizes sylvite 1-5 parts, 0.1-1.0 parts of sodium cetanesulfonate, dodecyl benzene sulfonic acid 0.01-
1.0 parts, 0.001-0.5 parts of sodium hydroxide, 40-70 parts of sodium hypochlorite, 22.5-58.889 parts of deionized water.
5. a kind of preparation method of chlorine-containing disinfectant according to claim 3, which is characterized in that the chlorine-containing disinfectant
PH value is controlled in 8.0-14.0.
6. a kind of preparation method of chlorine-containing disinfectant according to claim 3, which is characterized in that it is heavy to stand in step (3)
Dropping the time is 20-30 hours.
7. a kind of preparation method of chlorine-containing disinfectant according to claim 3, which is characterized in that filtering is adopted in step (3)
With 200 mesh filter clothes.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113197218A (en) * | 2021-03-30 | 2021-08-03 | 天圣制药集团股份有限公司 | Disinfectant and preparation method thereof |
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