CN109628441A - A kind of full-automatic nucleic acid purifier - Google Patents
A kind of full-automatic nucleic acid purifier Download PDFInfo
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- CN109628441A CN109628441A CN201811495036.6A CN201811495036A CN109628441A CN 109628441 A CN109628441 A CN 109628441A CN 201811495036 A CN201811495036 A CN 201811495036A CN 109628441 A CN109628441 A CN 109628441A
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 37
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 37
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 37
- 238000012360 testing method Methods 0.000 claims abstract description 155
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 84
- 238000011534 incubation Methods 0.000 claims abstract description 27
- 230000007246 mechanism Effects 0.000 claims abstract description 24
- 238000010438 heat treatment Methods 0.000 claims abstract description 6
- 230000003287 optical effect Effects 0.000 claims description 8
- 238000009423 ventilation Methods 0.000 claims description 8
- 230000005540 biological transmission Effects 0.000 claims description 5
- 238000009434 installation Methods 0.000 claims description 5
- 230000008878 coupling Effects 0.000 claims description 4
- 238000010168 coupling process Methods 0.000 claims description 4
- 238000005859 coupling reaction Methods 0.000 claims description 4
- 230000001360 synchronised effect Effects 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 abstract description 27
- 238000007689 inspection Methods 0.000 abstract description 11
- 238000001821 nucleic acid purification Methods 0.000 abstract description 11
- 239000012445 acidic reagent Substances 0.000 abstract description 10
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000001179 sorption measurement Methods 0.000 description 23
- 238000005336 cracking Methods 0.000 description 19
- 239000007788 liquid Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 8
- 230000006872 improvement Effects 0.000 description 7
- 238000005457 optimization Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000010009 beating Methods 0.000 description 4
- 238000007664 blowing Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000000746 purification Methods 0.000 description 3
- 239000000428 dust Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- QTCANKDTWWSCMR-UHFFFAOYSA-N costic aldehyde Natural products C1CCC(=C)C2CC(C(=C)C=O)CCC21C QTCANKDTWWSCMR-UHFFFAOYSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- ISTFUJWTQAMRGA-UHFFFAOYSA-N iso-beta-costal Natural products C1C(C(=C)C=O)CCC2(C)CCCC(C)=C21 ISTFUJWTQAMRGA-UHFFFAOYSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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Abstract
The invention discloses a kind of full-automatic nucleic acid purifiers, including workbench, rack for test tube, three-dimensional grips, incubate slot, centrifuge, work holder, refrigerated structure and high-temperature heating area, front and rear panels are respectively arranged at edges at two ends above the workbench, one end is arranged in parallel with sliding rail X-axis above the workbench, and described workbench one end is provided with incubation slot.The beneficial effects of the present invention are: the degree of automation that the present invention works is higher, use is time saving and energy saving, carries out the efficiency of nucleic acid purification faster, and the present invention can carry out the nucleic acid reagent purifying of a variety of different type inspections respectively, and suitability is high.Mounting plate at the top of test tube fixed mechanism is fastened with centrifugation motor spindle, and the rack for test tube in test tube fixed mechanism is made to keep 45 degree inclinations, and then is protected when centrifuge starts turning outside the reagent being mounted in rack for test tube will not be got rid of in rotation and spill, and environment is avoided pollution；The purifying instrument is easy to operate, has good social benefit, is suitble to promote the use of.
Description
Technical field
The present invention relates to a kind of purifying instrument, specially a kind of full-automatic nucleic acid purifier belongs to nucleic acid purifier application skill
Art field.
Background technique
With the fast development of people's living standard, nucleic acid purifier uses the people that more and more frequently, works in DNA detection
Member facilitates staff to carry out further by being incubated, being shaken, after centrifugal purification to nucleic acid reagent on nucleic acid purifier
DNA detection, guarantee detection accuracy.
Existing nucleic acid purifier remains many shortcomings when in use, and existing nucleic acid purifier is using
When need the test tube in artificial transfer rack for test tube incubated, shaken, centrifugal purification, when use, is time-consuming and laborious, and existing core
Acid purifying instrument takes time and effort to install, the low efficiency of work, and the rack for test tube in existing nucleic acid purifier in use can not be right
Test tube carries out individually independent placement, while easily causing test tube interior reagent to be write freely and easily generating the process shaken and collided, together
When before nucleic acid purification, need constantly to pass through invisible spectro nucleic acid hot wind and brush holding temperature, the examination in existing purifying instrument
When pipe support is passed through hot wind, heat distribution it is not uniform enough, and the centrifuge in existing nucleic acid purifier to nucleic acid reagent be centrifuged when,
Reagent is easily write freely and easily and causes environmental pollution out.
Summary of the invention
The object of the invention is that needing manually to shift rack for test tube when in use to solve existing nucleic acid purifier
In test tube incubated, shaken, centrifugal purification, when use, is time-consuming and laborious, and existing nucleic acid purifier takes time and effort to install,
The low efficiency of work, and the rack for test tube in existing nucleic acid purifier can not carry out individually independent put to test tube in use
It sets, while being shaken and the process of collision easily causes test tube interior reagent to be write freely and easily generating, while before nucleic acid purification, needed pair
Invisible spectro nucleic acid constantly passes through hot wind and brushes holding temperature, when the rack for test tube in existing purifying instrument is passed through hot wind, heat point
Cloth is not uniform enough, and the centrifuge in existing nucleic acid purifier to nucleic acid reagent be centrifuged when, reagent is easily write freely and easily and causes ring out
The problem of border is polluted, and propose a kind of full-automatic nucleic acid purifier.
The purpose of the present invention can be achieved through the following technical solutions: a kind of full-automatic nucleic acid purifier, including workbench,
Rack for test tube, three-dimensional grips incubate slot, centrifuge, work holder, refrigerated structure and high-temperature heating area, the workbench top
Front and rear panels are respectively arranged at edges at two ends, one end is arranged in parallel with sliding rail X-axis, and the sliding rail above the workbench
Sliding rail Y-axis is provided with below X-axis both ends, described workbench one end is provided with incubation slot, and the incubation slot internal sleeve is equipped with examination
Pipe support, and the incubation slot side is provided with centrifuge, and three-dimensional grips, the workbench middle part are mounted in the sliding rail X-axis
There are three the holders that works for setting, and there are three suction nozzle framves for side setting in the middle part of the workbench, and suction nozzle frame edge is set
Several reagent bottle racks are equipped with, and described workbench one end is provided with the refrigerated structure of indent, the refrigerated structure side
It is provided with the high-temperature heating area of indent；
Wherein, it is provided with stepper motor in the three-dimensional grips, and the stepper motor side is symmetrical arranged that there are two folders
Pawl, the stepper motor side are vertically arranged with electric pipettor；
The rack for test tube ventilation slot, support tube, fixing sucking disk, air inlet pipe, connecting tube, pipe cap, the first jack, the first rubber
Circle, the second jack, internal tube plate, the second rubber ring, outer tube and inner tube, surrounding offers ventilation slot on the outside of the rack for test tube, institute
The installation of rack for test tube bottom surrounding is stated there are four the support tube, four support bottom of the tube are mounted on fixing sucking disk, and four
The support tube is connected by connecting tube, and the connecting tube side is connected with air inlet pipe, and described air inlet pipe one end is equipped with
Pipe cap, the rack for test tube top are uniformly provided with several first jacks, are arranged on the inside of each first jack
First rubber ring, the outer pipe bottom are equipped with inner tube at the top of the first rubber ring, the rack for test tube and at the top of outer tube
Plate, corresponding first jack in said inner tube plate top are uniformly provided with several second jacks, and each described second inserts
The second rubber ring is arranged on the inside of hole, said inner tube bottom end passes through the second rubber ring and is mounted on the inside of outer tube；
It is cylindrical structure at the top of the centrifuge, and both ends are provided with the mounting groove of indent at the top of the centrifuge,
And hollow reagent fixed mechanism is embedded with inside the mounting groove；
Further technological improvement of the invention is: it is arc-shaped structure at the top of several described front and rear panels, and
Foreboard is symmetrical set with back plate, and front and rear panels cooperation, sealing state when making the purifying instrument overall work avoids rack for test tube
In reagent fall into dust impurity, improve the quality of nucleic acid purification.
Further technological improvement of the invention is: thermostat and pendulum shake connecting rod, and work are embedded with inside the incubation slot
It is internally provided with electrical cabinet as platform, electrical cabinet powers to each device of the purifying instrument.
Further technological improvement of the invention is: one end is passed by transmission optical axis connection between two sliding rail Y-axis
The junction of dynamic optical axis and sliding rail Y-axis is provided with shaft coupling, and is arranged with and sliding rail X-axis phase in two sliding rail Y-axis
The lower margin matched connect above the lower margin with sliding rail X-axis end by bolt, and sliding rail X-axis pass through synchronous belt and three-dimensional grips,
And sliding rail Y-axis is movably connected by the motor inside lower margin with sliding rail X-axis, guarantees that three-dimensional grips being capable of multidirectional adjusting shifting
It is dynamic.
Further technological improvement of the invention is: reagent fixed mechanism one end side wall is welded with cylindrical connection
Axis, and the reagent fixed mechanism bottom end is tiltedly installed with the mounting plate of rectangle.
Further technological improvement of the invention is: being provided with the cooperation to match with connecting shaft in the middle part of mounting groove both ends
Hole, connecting shaft are embedded in matching hole.
Further technological improvement of the invention is: being provided with motor below the centrifuge, the motor passes through shaft
It is rotatablely connected with centrifuge, motor drives the rack for test tube installed in centrifuge rotation to be centrifuged nucleic acid reagent by rotation.
Further technological improvement of the invention is: reagent fixed mechanism is inclined to set, and reagent fixed mechanism inclines
Rake angle is 45 degree, and then protects the reagent being mounted in rack for test tube not spilt by getting rid of in rotation when centrifuge starts turning
Out, environment is avoided pollution, and guarantees the stability that nucleic acid reagent is centrifuged.
Compared with prior art, the beneficial effects of the present invention are:
1, the degree of automation for working of the present invention is higher, and use is time saving and energy saving, carries out the efficiency of nucleic acid purification faster, and this
Invention can carry out the nucleic acid reagent purifying of a variety of different type inspections respectively, and suitability is high.
2, the fixing sucking disk on the rack for test tube in the present invention, which is adsorbed on table top, is fixed, while passing through the first rubber pad
Fixed outer tube, the fixed inner tube of the second rubber pad, buffers and fixes test tube in collision and shaking, avoid interior reagent from writing freely and easily, together
When each test tube individually stored in the main body of the tube, effectively classify, be easy to use；The main body of the tube surrounding is equal simultaneously
Ventilation slot is offered, is constantly brushed convenient for hot wind and is blown into rack for test tube, internal temperature is maintained, is distributed heat more in rack for test tube
Uniformly.
3, rack for test tube is put by the test tube fixed mechanism set at centrifuge both ends, the connecting shaft on test tube fixed mechanism is embedded
In matching hole, cooperates mounting plate and centrifugation motor spindle at the top of test tube fixed mechanism to fasten, make the test tube in test tube fixed mechanism
Frame keeps 45 degree of inclinations, and then protects the reagent being mounted in rack for test tube not got rid of in rotation when centrifuge starts turning
Outside spilling, environment is avoided pollution, and guarantees the stability that nucleic acid reagent is centrifuged；The purifying instrument is easy to operate, has good
Social benefit is suitble to promote the use of.
Detailed description of the invention
In order to facilitate the understanding of those skilled in the art, the present invention will be further described below with reference to the drawings.
Fig. 1 is entirety perspective view of the present invention.
Fig. 2 is stereochemical structure decomposing schematic representation of the present invention.
Fig. 3 is the stereochemical structure connection schematic diagram of sliding rail X-axis of the present invention and electric pipettor.
Fig. 4 is sliding rail Y-axis of the present invention and the connection schematic diagram for being driven optical axis.
Fig. 5 is a-quadrant detail enlarged diagram in Fig. 1 of the present invention.
Fig. 6 is the schematic perspective view of rack for test tube of the present invention.
Fig. 7 is the inclination schematic diagram of rack for test tube of the present invention.
Fig. 8 is the stereochemical structure decomposing schematic representation of rack for test tube of the present invention.
Fig. 9 is centrifuge schematic perspective view of the present invention.
Figure 10 is the top view of centrifuge of the present invention.
Figure 11 is the schematic perspective view of centrifuge of the present invention.
Figure 12 is the schematic perspective view of reagent fixed mechanism of the present invention.
Figure 13 is the bottom substance schematic diagram of reagent fixed mechanism of the present invention.
Figure 14 is the top view of mounting plate of the present invention.
Figure 15 is the front view of centrifuge of the present invention.
In figure: 1, workbench；2, rack for test tube；3, foreboard；4, back plate；5, sliding rail X-axis；6, sliding rail Y-axis；10, slot is incubated；
11, centrifuge；14, three-dimensional grips；15, stepper motor；16, clamping jaw；17, electric pipettor；18, it is driven optical axis；20, shaft coupling
Device；21, lower margin；22, work holder；25, suction nozzle frame；26, reagent bottle rack；27, refrigerated structure；28, it is heated at high temperature area；
113, mounting groove；114, reagent fixed mechanism；1144, connecting shaft；1148, mounting plate；116, matching hole；117, shaft；118,
Motor；202, ventilation slot；203, support tube；204, fixing sucking disk；205, air inlet pipe；206, connecting tube；207, pipe cap；208,
One jack；209, the first rubber ring；210, the second jack；211, internal tube plate；212, the second rubber ring；213, outer tube；214, interior
Pipe.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality
Applying example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff all other embodiment obtained without creative efforts belongs to what the present invention protected
Range.
Please refer to shown in Fig. 1-15, a kind of full-automatic nucleic acid purifier, including workbench 1, rack for test tube 2, three-dimensional grips 14,
It incubates slot 10, centrifuge 11, work holder 22, refrigerated structure 27 and is heated at high temperature area 28, at 1 top edges at two ends of workbench
It is respectively arranged with foreboard 3 and back plate 4,1 top one end of workbench is arranged in parallel with sliding rail X-axis 5, and below 5 both ends of sliding rail X-axis
It is provided with sliding rail Y-axis 6,1 one end of workbench, which is provided with, incubates slot 10, incubates 10 internal sleeve of slot and is equipped with rack for test tube 2, and incubates slot 10
Side is provided with centrifuge 11, three-dimensional grips 14 is mounted in sliding rail X-axis 5, there are three the holders that works for setting in the middle part of workbench 1
22, and there are three suction nozzle framves 25 for the setting of 1 middle part side of workbench, 25 edge of suction nozzle frame is provided with several reagent bottle racks
26, and 1 one end of workbench is provided with the refrigerated structure 27 of indent, 27 side of refrigerated structure is provided with the high-temperature heating area of indent
28；
Wherein, it is provided with stepper motor 15 in three-dimensional grips 14, and 15 side of stepper motor is symmetrical arranged that there are two clamping jaws
16,15 side of stepper motor is vertically arranged with electric pipettor 17；
2 ventilation slot 202 of rack for test tube, support tube 203, fixing sucking disk 204, air inlet pipe 205, connecting tube 206, pipe cap 207,
One jack 208, the first rubber ring 209, the second jack 210, internal tube plate 211, the second rubber ring 212, outer tube 213 and inner tube 214,
2 outside surrounding of rack for test tube offers ventilation slot 202, and there are four support tube 203, four supports for the installation of 2 bottom surrounding of rack for test tube
203 bottom of pipe is mounted on fixing sucking disk 204, and four support tubes 203 are connected by connecting tube 206, the connection of 206 side of connecting tube
Be provided with air inlet pipe 205,205 one end of air inlet pipe is equipped with pipe cap 207, be uniformly provided at the top of rack for test tube 2 several first insert
Hole 208, each first jack, 208 inside are arranged with the first rubber ring 209, and 213 bottom of outer tube passes through the first rubber ring 209,
Internal tube plate 211 is installed, corresponding first jack 208 is uniform at the top of internal tube plate 211 at the top of rack for test tube 2 and at the top of outer tube 213
Several the second jacks 210 are offered, each second jack, 210 inside is arranged with the second rubber ring 212,214 bottom end of inner tube
213 inside of outer tube is mounted on across the second rubber ring 212；
It is cylindrical structure at the top of centrifuge 11, and 11 top both ends of centrifuge are provided with the mounting groove 113 of indent, and
Hollow reagent fixed mechanism 114 is embedded with inside mounting groove 113；
It is arc-shaped structure at the top of several foreboards 3 and back plate 4 as a kind of technical optimization scheme of the invention, and
Foreboard 3 is symmetrical set with back plate 4, and foreboard 3 and back plate 4 cooperate, and sealing state when making the purifying instrument overall work avoids trying
Reagent in pipe support 2 falls into dust impurity, improves the quality of nucleic acid purification.
As a kind of technical optimization scheme of the invention, incubates and be embedded with thermostat and pendulum shake connecting rod inside slot 10, and work
Make platform 1 and be internally provided with electrical cabinet, electrical cabinet powers to each device of the purifying instrument.
As a kind of technical optimization scheme of the invention, one end is connected by transmission optical axis 18 between two sliding rail Y-axis 6,
The junction of transmission optical axis 18 and sliding rail Y-axis 6 is provided with shaft coupling 20, and is arranged with and sliding rail X-axis 5 in two sliding rail Y-axis 6
The lower margin 21 to match is connect above lower margin 21 with 5 end of sliding rail X-axis by bolt, and sliding rail X-axis 5 passes through synchronous belt and three
The motor that dimension fixture 14 and sliding rail Y-axis 9 pass through inside lower margin 4 is movably connected with sliding rail X-axis 5, guarantees 14 energy of three-dimensional grips
It is more than enough to be moved to adjusting.
As a kind of technical optimization scheme of the invention, 114 one end side wall of reagent fixed mechanism is welded with cylindrical company
Spindle 1144, and 114 bottom end of reagent fixed mechanism is tiltedly installed with the mounting plate 1148 of rectangle.
As a kind of technical optimization scheme of the invention, it is provided with and 1144 phase of connecting shaft in the middle part of 113 both ends of mounting groove
Matched matching hole 116, connecting shaft 1144 are embedded in matching hole 116.
As a kind of technical optimization scheme of the invention, motor 118 is provided with below centrifuge 11, motor 118 is by turning
Axis 117 and centrifuge 11 are rotatablely connected, and motor 118 drives the rack for test tube 2 installed in centrifuge 11 to rotate to core by rotation 117
Acid reagent centrifugation.
As a kind of technical optimization scheme of the invention, reagent fixed mechanism 114 is inclined to set, and reagent fixed mechanism
114 tilt angle is 45 degree, so protect when centrifuge starts turning the reagent that is mounted in rack for test tube 2 when rotating not
It can be got rid of outside spilling, avoid pollution environment, and guarantee the stability that nucleic acid reagent is centrifuged.
Embodiment 1
Conventional inspection pollution inspection inspection process:
Sample is put into the reagent bottle of reagent bottle rack 26 → takes by No.1 rack for test tube to be put into outside 2 upper layer of No.1 rack for test tube
It sets in cracking tube, the program editted by this purifying instrument, purifies the electricity in the PLC controller control three-dimensional grips 14 inside instrument
Dynamic pipettor 17 draws reagent, and 320 μ L of No. 3 reagent bottle reagents → by stepper motor 15 in three-dimensional grips 14 is added and drives clamping jaw
16 crawl rack for test tubes 2, which are put it into, to be incubated in slot 10, is vibrated by pendulum shake connecting rod to rack for test tube 2, by thermostat to rack for test tube 2
56 DEG C of incubation temperature, the time: rack for test tube 2 is sandwiched centrifugal rotational speed in centrifuge by 15min → by who fixture 14: 12000
Turn/1min, at this time DNA in the big pipe of 2 cylinder of No.1 rack for test tube → 2 upper layer cracking tube whole plate of No.1 rack for test tube taken away → by one
Number big liquid in pipe of 2 cylinder of rack for test tube is successively transferred in No. two external cracking tubes in 2 upper layer of rack for test tube → and 12000 turns of centrifugation/
1min, at this time DNA in No. two big pipes of 2 cylinder of rack for test tube → No. two external cracking tube whole plates in 2 upper layer of rack for test tube are taken away → to
First be added in No. two big pipes of 2 cylinder of rack for test tube after 600 μ L of No. 2 bottle reagents be added then 10 μ L of kingpin reagent is blown and beaten after mix →
No. two big liquid in pipe of rack for test tube cylinder are successively transferred in No. three rack for test tube upper layer adsorption tubes → it is centrifuged 6000 turns/3min again
Centrifugation 12000 turns/1min → sequentially adds 450 μ L of headpin reagent → 12000 turns/1min of centrifugation → headpin reagent is added again
450 μ L → 12000 turns/1min of centrifugation is centrifuged the DNA of 12000 turns/3min → at this time in No. three rack for test tube upper layer adsorption tubes again, will
No. three rack for test tube upper layer adsorption tube whole plates are transferred in No. four big tube layer of rack for test tube cylinder → incubation temperature: 75 DEG C, the time:
3min；Effect is volatilization rinsing AW → sequentially add No. 4 bottle reagent A E10 μ L → incubation temperatures to adsorption tube: 75 DEG C, the time:
12000 turns/1min of 3min → centrifugation at this time DNA in No. four big pipes of rack for test tube cylinder → upper layer adsorption tube whole plate is taken away after its
No. four pipe supports of remaininging are put into refrigerated structure 27, complete nucleic acid purification.
Embodiment 2:
Bone examines inspection process: No.1 rack for test tube is put into the reagent bottle of reagent bottle rack 26 → take sample to be put into one
In number external cracking tube in 2 upper layer of rack for test tube, the program editted by this purifying instrument purifies the PLC controller inside instrument and passes through
Electric pipettor 17 in three-dimensional grips 14 draws reagent, and 320 μ L of No. 3 reagent bottle reagents → by walking in three-dimensional grips 14 is added
It drives clamping jaw 16 to grab rack for test tube 2 into motor 15 to put it into incubation slot 10, vibrate rack for test tube 2 by pendulum shake connecting rod, lead to
Thermostat being crossed to 56 DEG C of 2 incubation temperature of rack for test tube, the time, rack for test tube 2 is sandwiched centrifuge by: 15min → by who fixture 14
Middle centrifugal rotational speed: 12000 turns/1min, at this time DNA in the big pipe of 2 cylinder of No.1 rack for test tube → by 2 upper layer of No.1 rack for test tube crack
Pipe whole plate is taken away → the big liquid in pipe of 2 cylinder of No.1 rack for test tube is successively transferred in No. two external cracking tubes in 2 upper layer of rack for test tube
12000 turns/1min of → centrifugation, at this time DNA in No. two big pipes of 2 cylinder of rack for test tube → by No. two external cracking in 2 upper layer of rack for test tube
Addition 10 μ L of kingpin reagent is right after 600 μ L of No. 2 bottle reagents is taken → be first added into No. two big pipes of 2 cylinder of rack for test tube to pipe whole plate away
Mixed after blowing and beating afterwards → No. two big liquid in pipe of rack for test tube cylinder are successively transferred in No. three rack for test tube upper layer adsorption tubes → from
6000 turns/3min of the heart is centrifuged 12000 turns/1min → again and sequentially adds 450 μ L of headpin reagent → 12000 turns/1min of centrifugation → again
Secondary 450 μ L of addition headpin reagent → 12000 turns/1min of centrifugation is centrifuged the DNA of 12000 turns/3min → at this time in No. three rack for test tubes again
In the adsorption tube of upper layer, No. three rack for test tube upper layer adsorption tube whole plates are transferred in No. four big tube layer of rack for test tube cylinder → are incubated temperature
Degree: 75 DEG C, the time: 3min；Effect sequentially adds No. 4 bottle reagent A E10 μ L → incubation temperature for volatilization rinsing AW → to adsorption tube
Degree: 75 DEG C, the time: 12000 turns/1min of 3min → centrifugation at this time DNA in No. four big pipes of rack for test tube cylinder → upper layer is adsorbed
Remaining No. four pipe support is put into refrigerated structure 27 after pipe whole plate is taken away, completes nucleic acid purification.
Embodiment 3
Costal cartilage hair examines inspection process: No.1 rack for test tube is put into the reagent bottle of reagent bottle rack 26 → take sample
It is put into the external cracking tube in 2 upper layer of No.1 rack for test tube, the program editted by this purifying instrument, purifies the PLC control inside instrument
Device draws reagent by the electric pipettor 17 in three-dimensional grips 14, and 320 μ L of No. 3 reagent bottle reagents → pass through three-dimensional grips is added
Stepper motor 15 drives clamping jaw 16 to grab rack for test tube 2 and puts it into incubation slot 10 on 14, is shaken by pendulum shake connecting rod to rack for test tube 2
Who swing, by thermostat to 56 DEG C of 2 incubation temperature of rack for test tube, time: 15min → sandwiched rack for test tube 2 by fixture 14
Centrifugal rotational speed in centrifuge: 12000 turns/1min, at this time DNA in the big pipe of 2 cylinder of No.1 rack for test tube → will be on No.1 rack for test tube 2
Floor cracking tube whole plate takes → is successively transferred to the big liquid in pipe of 2 cylinder of No.1 rack for test tube that No. two 2 upper layers of rack for test tube are external to be split away
Solution pipe in → centrifugation 12000 turns/1min, at this time DNA in No. two big pipes of 2 cylinder of rack for test tube → will be outside No. two 2 upper layers of rack for test tube
It sets after 600 μ L of No. 2 bottle reagents is taken → be first added into No. two big pipes of 2 cylinder of rack for test tube to cracking tube whole plate away and kingpin reagent is added
10 μ L mix after then blowing and beating → No. two big liquid in pipe of rack for test tube cylinder are successively transferred to No. three rack for test tube upper layer adsorption tubes
It is interior → centrifugation 6000 turns/3min be centrifuged again 12000 turns/1min → sequentially add 450 μ L of headpin reagent → 12000 turns of centrifugation/
1min → again is added 450 μ L of headpin reagent → 12000 turns/1min of centrifugation and is centrifuged the DNA of 12000 turns/3min → at this time again three
In number rack for test tube upper layer adsorption tube, No. three rack for test tube upper layer adsorption tube whole plates are transferred in No. four big tube layer of rack for test tube cylinder →
Incubation temperature: 75 DEG C, the time: 3min；Effect sequentially adds No. 4 bottle reagent A E10 μ L → temperature for volatilization rinsing AW → to adsorption tube
Educate temperature: 75 DEG C, the time: 12000 turns/1min of 3min → centrifugation at this time DNA in No. four big pipes of rack for test tube cylinder → by upper layer
Remaining No. four pipe support is put into refrigerated structure 27 after adsorption tube whole plate is taken away, completes nucleic acid purification.
Embodiment 4
Concentration inspection inspection process: No.1 rack for test tube is put into the reagent bottle of reagent bottle rack 26 → take sample to be put into one
In number external cracking tube in 2 upper layer of rack for test tube, the program editted by this purifying instrument purifies the PLC controller inside instrument and passes through
Electric pipettor 17 in three-dimensional grips 14 draws reagent, and 320 μ L of No. 3 reagent bottle reagents → by walking in three-dimensional grips 14 is added
It drives clamping jaw 16 to grab rack for test tube 2 into motor 15 to put it into incubation slot 10, vibrate rack for test tube 2 by pendulum shake connecting rod, lead to
Thermostat being crossed to 56 DEG C of 2 incubation temperature of rack for test tube, the time, rack for test tube 2 is sandwiched centrifuge by: 15min → by who fixture 14
Middle centrifugal rotational speed: 12000 turns/1min, at this time DNA in the big pipe of 2 cylinder of No.1 rack for test tube → by 2 upper layer of No.1 rack for test tube crack
Pipe whole plate is taken away → the big liquid in pipe of 2 cylinder of No.1 rack for test tube is successively transferred in No. two external cracking tubes in 2 upper layer of rack for test tube
12000 turns/1min of → centrifugation, at this time DNA in No. two big pipes of 2 cylinder of rack for test tube → by No. two external cracking in 2 upper layer of rack for test tube
Addition 10 μ L of kingpin reagent is right after 600 μ L of No. 2 bottle reagents is taken → be first added into No. two big pipes of 2 cylinder of rack for test tube to pipe whole plate away
Mixed after blowing and beating afterwards → No. two big liquid in pipe of rack for test tube cylinder are successively transferred in No. three rack for test tube upper layer adsorption tubes → from
6000 turns/3min of the heart is centrifuged 12000 turns/1min → again and sequentially adds 450 μ L of headpin reagent → 12000 turns/1min of centrifugation → again
Secondary 450 μ L of addition headpin reagent → 12000 turns/1min of centrifugation is centrifuged the DNA of 12000 turns/3min → at this time in No. three rack for test tubes again
In the adsorption tube of upper layer, No. three rack for test tube upper layer adsorption tube whole plates are transferred in No. four big tube layer of rack for test tube cylinder → are incubated temperature
Degree: 75 DEG C, the time: 3min；Effect sequentially adds No. 4 bottle reagent A E10 μ L → incubation temperature for volatilization rinsing AW → to adsorption tube
Degree: 75 DEG C, the time: 12000 turns/1min of 3min → centrifugation at this time DNA in No. four big pipes of rack for test tube cylinder → upper layer is adsorbed
Remaining No. four pipe support is put into refrigerated structure 27 after pipe whole plate is taken away, completes nucleic acid purification.
Embodiment 5
CHXLXE examines inspection process: sample is put into the external cracking tube in No.1 rack for test tube upper layer → it is manually added CHXLXE
No.1 rack for test tube → is put into and is incubated in slot 10 → concussion incubation temperature by overlay film after reagent: 56 DEG C, the time: 30min or more → use
Rack for test tube is transferred to by three-dimensional grips 14 is heated at high temperature 28 high temperature incubation temperature of area: 95 DEG C, the time: 10min → centrifugation 12000
Turn/1min is at this time in the big pipe of DNA No.1 rack for test tube cylinder → to take the external cracking tube whole plate in upper layer away, remaining No.1 pipe support is put into
Refrigerated structure 27.
Embodiment 6
Property invade inspection and retain women composition detection process: No.1 rack for test tube is put into the reagent bottle of reagent bottle rack 26 →
Sample is taken to be put into the external cracking tube in 2 upper layer of No.1 rack for test tube, the program editted by this purifying instrument purifies inside instrument
PLC controller draws reagent by the electric pipettor 17 in three-dimensional grips 14, and 320 μ L of No. 3 reagent bottle reagents → pass through is added
Stepper motor 15 drives clamping jaw 16 to grab rack for test tube 2 and puts it into incubation slot 10 in three-dimensional grips 14, passes through pendulum and shakes connecting rod pair
Rack for test tube 2 vibrates, by thermostat to 56 DEG C of 2 incubation temperature of rack for test tube, time: 15min → will be tried by who fixture 14
Pipe support 2 sandwiches centrifugal rotational speed in centrifuge: 12000 turns/1min, at this time DNA in the big pipe of 2 cylinder of No.1 rack for test tube → by No.1
2 upper layer cracking tube whole plate of rack for test tube is taken away → the big liquid in pipe of 2 cylinder of No.1 rack for test tube is successively transferred on No. two rack for test tubes 2
In the external cracking tube of layer → 12000 turns/1min of centrifugation, at this time DNA in No. two big pipes of 2 cylinder of rack for test tube → by No. two rack for test tubes
The external cracking tube whole plate in 2 upper layers is added No. 5 after taking → being first added into No. two big pipes of 2 cylinder of rack for test tube 600 μ L of No. 2 bottle reagents away
Bottle 10 μ L of reagent mixes after then blowing and beating → No. two big liquid in pipe of rack for test tube cylinder are successively transferred to No. three rack for test tube upper layers
In adsorption tube → 6000 turns/3min of centrifugation is centrifuged 12000 turns/1min → again and sequentially adds 450 μ L of headpin reagent → centrifugation
12000 turns/1min → again be added 450 μ L of headpin reagent → 12000 turns/1min of centrifugation be centrifuged again 12000 turns/3min → this
When DNA in No. three rack for test tube upper layer adsorption tubes, No. three rack for test tube upper layer adsorption tube whole plates are transferred to No. four rack for test tube cylinders
In big tube layer → and incubation temperature: 75 DEG C, the time: 3min；Effect sequentially adds No. 4 bottle reagents for volatilization rinsing AW → to adsorption tube
AE10 μ L → incubation temperature: 75 DEG C, the time: 12000 turns/1min of 3min → centrifugation at this time DNA in No. four big pipes of rack for test tube cylinder
It is interior → upper layer adsorption tube whole plate is taken away after remaining No. four pipe support be put into refrigerated structure 27, complete nucleic acid purification.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (8)
1. a kind of full-automatic nucleic acid purifier, it is characterised in that: including workbench (1), rack for test tube (2), three-dimensional grips (14), temperature
Educate slot (10), centrifuge (11), work holder (22), refrigerated structure (27) and high-temperature heating area (28), the workbench (1)
Foreboard (3) and back plate (4) are respectively arranged at the edges at two ends of top, one end is arranged in parallel with sliding rail X above the workbench (1)
Axis (5), and be provided with sliding rail Y-axis (6) below the sliding rail X-axis (5) both ends, described workbench (1) one end is provided with incubation
Slot (10), incubation slot (10) internal sleeve is equipped with rack for test tube (2), and incubation slot (10) side is provided with centrifuge
(11), it is mounted with three-dimensional grips (14) on the sliding rail X-axis (5), there are three the holders that works for setting in the middle part of the workbench (1)
(22), and in the middle part of the workbench (1) there are three suction nozzle frame (25) for side setting, and the suction nozzle frame (25) is if edge is provided with
Dry reagent bottle rack (26), and the workbench (1) one end is provided with the refrigerated structure (27) of indent, the refrigerated structure
(27) side is provided with the high-temperature heating area (28) of indent；
Wherein, it is provided with stepper motor (15) on the three-dimensional grips (14), and the stepper motor (15) side is symmetrical arranged
There are two clamping jaw (16), and stepper motor (15) side is vertically arranged with electric pipettor (17)；
Rack for test tube (2) ventilation slot (202), support tube (203), fixing sucking disk (204), air inlet pipe (205), connecting tube
(206), pipe cap (207), the first jack (208), the first rubber ring (209), the second jack (210), internal tube plate (211), second
Rubber ring (212), outer tube (213) and inner tube (214), rack for test tube (2) outside surrounding offer ventilation slot (202), institute
Stating the installation of rack for test tube (2) bottom surrounding, there are four the support tube (203), and four support tube (203) bottoms are mounted on
Fixing sucking disk (204), four support tubes (203) are connected by connecting tube (206), connecting tube (206) the side connection
It is provided with air inlet pipe (205), described air inlet pipe (205) one end is equipped with pipe cap (207), is uniformly opened at the top of the rack for test tube (2)
Equipped with the first jack (208) described in several, the first rubber ring (209) are arranged on the inside of each first jack (208),
Outer tube (213) bottom passes through the first rubber ring (209), at the top of the rack for test tube (2) and is located at installation at the top of outer tube (213)
Have internal tube plate (211), at the top of said inner tube plate (211) corresponding first jack (208) be uniformly provided with several described the
Two jacks (210), each second jack (210) inside are arranged with the second rubber ring (212), said inner tube (214) bottom
End is mounted on the inside of outer tube (213) across the second rubber ring (212)；
It is cylindrical structure at the top of the centrifuge (11), and both ends are provided with the installation of indent at the top of the centrifuge (11)
Slot (113), and hollow reagent fixed mechanism (114) is embedded with inside the mounting groove (113).
2. a kind of full-automatic nucleic acid purifier according to claim 1, which is characterized in that several described foreboards (3) and
It is arc-shaped structure at the top of back plate (4), and foreboard (3) is symmetrical set with back plate (4).
3. a kind of full-automatic nucleic acid purifier according to claim 1, which is characterized in that the incubation slot (10) is internal embedding
Equipped with thermostat and pendulum shake connecting rod, and workbench (1) is internally provided with electrical cabinet.
4. a kind of full-automatic nucleic acid purifier according to claim 1, which is characterized in that two sliding rail Y-axis (6) it
Between one end shaft coupling (20) is provided with by transmission optical axis (18) connection, transmission optical axis (18) and the junction of sliding rail Y-axis (6),
And the lower margin (21) to match with sliding rail X-axis (5) is arranged on two sliding rail Y-axis (6), it is logical above the lower margin (21)
It crosses bolt to connect with sliding rail X-axis (5) end, and sliding rail X-axis (5) passes through synchronous belt and three-dimensional grips (14) and sliding rail Y-axis (9)
It is movably connected by the internal motor of lower margin (4) with sliding rail X-axis (5).
5. a kind of full-automatic nucleic acid purifier according to claim 1, which is characterized in that the reagent fixed mechanism
(114) one end side wall is welded with cylindrical connecting shaft (1144), and reagent fixed mechanism (114) bottom end inclination is set
It is equipped with the mounting plate (1148) of rectangle.
6. a kind of full-automatic nucleic acid purifier according to claim 5, which is characterized in that in the middle part of mounting groove (113) both ends
It is provided with the matching hole (116) to match with connecting shaft (1144), connecting shaft (1144) is embedded in matching hole (116).
7. a kind of full-automatic nucleic acid purifier according to claim 1, which is characterized in that set below the centrifuge (11)
It is equipped with motor (118), the motor (118) is rotatablely connected by shaft (117) and centrifuge (11).
8. a kind of full-automatic nucleic acid purifier according to claim 1, which is characterized in that the reagent fixed mechanism
(114) it is inclined to set, and the tilt angle of reagent fixed mechanism (114) is 45 degree.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811495036.6A CN109628441A (en) | 2018-12-07 | 2018-12-07 | A kind of full-automatic nucleic acid purifier |
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| CN201811495036.6A CN109628441A (en) | 2018-12-07 | 2018-12-07 | A kind of full-automatic nucleic acid purifier |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112375756A (en) * | 2020-11-12 | 2021-02-19 | 安徽森芃生物科技有限责任公司 | Method for fully-automatic nucleic acid extraction aiming at solid sample |
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Effective date of registration: 20191205 Address after: 230000 8316, 3rd floor, R & D building, No. 5, Gushui Road, Anhui Changfeng Shuangfeng Economic Development Zone, Hefei City, Anhui Province Applicant after: Anhui senpeng Biotechnology Co.,Ltd. Address before: Heyang city of Rongcheng West Weihai 264300 Shandong Province Applicant before: SHANDONG SENPENG BIOTECHNOLOGY CO.,LTD. |
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