CN109603929A - Manufacturing method of valve on chip, microfluidic chip and liquid flow control method - Google Patents
Manufacturing method of valve on chip, microfluidic chip and liquid flow control method Download PDFInfo
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- CN109603929A CN109603929A CN201811330430.4A CN201811330430A CN109603929A CN 109603929 A CN109603929 A CN 109603929A CN 201811330430 A CN201811330430 A CN 201811330430A CN 109603929 A CN109603929 A CN 109603929A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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- General Health & Medical Sciences (AREA)
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Abstract
本发明涉及微流控芯片领域，本发明公开了一种芯片上阀门的制造方法、微流控芯片与液体流动控制方法，芯片制造方法包括以下步骤：在多孔介质基材上设置流道；使蜡液渗透入位于流道上的阀门区域处的多孔介质基材内；待蜡液完全渗透多孔介质基材并经过固化后形成阀门，阀门将流道分隔为互不连通的至少两个区域。本发明同样可以实现流道的阻隔与连通，同时其结构更为简单，操作方便，成本低廉，有助于微流控芯片的推广。
The invention relates to the field of microfluidic chips, and discloses a manufacturing method of a valve on a chip, a microfluidic chip and a liquid flow control method. The chip manufacturing method includes the following steps: setting a flow channel on a porous medium substrate; making The wax liquid penetrates into the porous medium substrate at the valve area on the flow channel; after the wax liquid completely penetrates the porous medium substrate and is cured, a valve is formed, and the valve divides the flow channel into at least two areas that are not connected to each other. The invention can also realize the blocking and communication of the flow channel, and meanwhile, the structure is simpler, the operation is convenient, and the cost is low, which is helpful for the popularization of the microfluidic chip.
Description
Technical field
The present invention relates to micro-fluidic chip field, more particularly, to the micro-fluidic chip field based on porous media substrate,
It is specifically related to flow control method of the liquid on micro-fluidic chip, and realizes the micro-fluidic chip of this method.
Background technique
Based on the micro-fluidic chip of porous media substrate, for example paper substrate micro-fluidic chip is a kind of novel microfluidic analysis
Device, because it has the characteristics that at low cost, the processing is simple, easy to use, therefore in care diagnostic, food quality control and environment prison
There is huge application prospect in the fields such as survey, are increasingly becoming the research hotspot in micro-fluidic chip field in recent years.
When a variety of reactants or reagent participate in detection, generally require to flow in runner using valve to control liquid
Precedence.In addition, being received significant attention in recent years using length method in the method that paper base chip is detected, principle is inspection
Substrate is surveyed after the conversion zone and predetermined substance of chip chemically react, reaction product is logical as mixed solution flows into miniflow
Road, and chromogenic reaction occurs with the color developing agent on channel.After the completion of solution flowing, certain length can be observed in channel
Develop the color section, and then can determine the content of reaction product and detectable substance.The device that this method uses is easy, easy to operate, but
It is in accuracy there is limitation usually will appear reactant not yet in being related to detection process that is secondary or repeatedly reacting
Reaction flows into microchannel completely, influences testing result.It, can be with by the way that hydrophobic valve is arranged in conversion zone and microchannel
Control is realized to the flowing of substrate solution, allows substrate sufficiently to react in conversion zone, the solution after fully reacting enters micro-
Circulation road carries out subsequent reaction again.Therefore, controlled using valve liquid flowing in paper base chip is that it is answered in detection field
Key technology.
The effect of valve is the flowing of control reaction solution, guarantees that detectable substance is reacted completely, and can react in the first step
Valve is opened after the completion, and solution is made to can smoothly enter into channel.Its key is to guarantee the closing and opening of valve.It deposits in the prior art
Valve closure is reached by the method folded and unfolded such as the three-dimensional paper chip detection device of Pop-up in a variety of valve mechanisms
Effect, change the flow path of detectable substance.The method for another example moved using electric textile material as valve controlling flow, positive reason
Detectable substance solution cannot flow through textile material under condition, and the method by applying potential between solution and valve enables solution
Enough barrier fabrics achieve the effect that open valve, however above-mentioned apparatus all has the shortcomings that structure is complicated, at high cost, it is difficult to full
The demand of sufficient people.
Summary of the invention
For overcome the deficiencies in the prior art, the present invention proposes a kind of manufacturing method of valve on chip, existing for solving
There are valve device structure complexity, problem at high cost.
The present invention also proposes a kind of micro-fluidic chip and liquid flow control method.
The present invention solves scheme used by its technical problem:
The manufacturing method of valve on a kind of chip, comprising the following steps:
Runner is set on porous media substrate；
Infiltrate through wax liquor in the porous media substrate at the valve region on runner；
Porous media substrate is permeated completely after wax liquor and forms valve after solidification, and runner is divided by valve not to be connected mutually
At least two logical regions.
Mode as a further improvement of the foregoing solution, the valve region on porous media substrate applies wax, then right
Porous media substrate is heated, so that wax is melted into wax liquor.
Mode as a further improvement of the foregoing solution applies wax in valve region by way of printing.
Runner is divided into mutual disconnected conversion zone and detection by mode as a further improvement of the foregoing solution, valve
Region.
A kind of micro-fluidic chip is made by the manufacturing method of valve in said chip.
A kind of liquid flow control method based on above-mentioned micro-fluidic chip a, comprising the following steps: region wherein
Interior addition reagent removes the wax of valve region after meeting and imposing a condition so that reagent flowed to from current region it is adjacent another
A region.
Mode as a further improvement of the foregoing solution, the method for removing the wax of valve region are as follows: apply to valve solvable
Solve the solvent of wax.
Mode as a further improvement of the foregoing solution, solvent are acetone, isopropanol, toluene, acetonitrile or n-hexane.
Mode as a further improvement of the foregoing solution imposes a condition are as follows: the reagent in current region has completely reacted
Or it is thoroughly mixed.
The beneficial effects of the present invention are:
Compared with the prior art the present invention equally may be implemented the barrier of runner be connected to, while its structure is more simple,
It is easy to operate, it is low in cost, facilitate the popularization of micro-fluidic chip.
Detailed description of the invention
Invention is further explained with reference to the accompanying drawings and examples.
Fig. 1 is the flow chart of micro-fluidic chip manufacturing method embodiment of the present invention；
Fig. 2 is the front view of micro-fluidic chip embodiment of the present invention；
Fig. 3 is that different solvents fully open volume histograms needed for valve；
Fig. 4 is that different solvents are completely dissolved histogram the time required to valve；
Fig. 5 is influence schematic diagram of the valve transparency to valve barriering effect.
Specific embodiment
It is carried out below with reference to technical effect of the embodiment and attached drawing to design of the invention, specific structure and generation clear
Chu, complete description, to be completely understood by the purpose of the present invention, scheme and effect.It should be noted that the case where not conflicting
Under, the features in the embodiments and the embodiments of the present application can be combined with each other.
It should be noted that unless otherwise specified, when a certain feature referred to as " fixation ", " connection " are in another feature,
It can directly fix, be connected to another feature, and can also fix, be connected to another feature indirectly.In addition, this
The descriptions such as up, down, left, right, before and after used in invention are only relative to the mutual of each component part of the invention in attached drawing
For positional relationship.
In addition, unless otherwise defined, the technology of all technical and scientific terms used herein and the art
The normally understood meaning of personnel is identical.Term used in the description is intended merely to description specific embodiment herein, without
It is to limit the present invention.Term " and or " used herein includes the arbitrary of one or more relevant listed items
Combination.
The invention discloses a kind of micro-fluidic chip manufacturing methods with wax valve, and based on the micro-fluidic chip
Liquid flow control method, central scope are the wax valves that setting infiltrates through inside porous media substrate on runner, work as valve
In the presence of door, runner is divided at least two regions, to realize complete reaction of the reagent in a region or complete
Mixing；And wax valve can quickly be removed by organic solvent, so that the connection between region is realized, under entering reagent
One region participates in reaction, to realize the detection of reagent.Compared with the prior art, the resistance of runner equally may be implemented in the present invention
Every be connected to, while its structure is more simple, easy to operate, low in cost, facilitates the popularization of micro-fluidic chip.
It is illustrated below by way of specific embodiment.
Referring to Fig.1, the flow chart of micro-fluidic chip manufacturing method one embodiment of the present invention is shown comprising following tool
Body step:
Step 1
Runner is set on porous media substrate, and the porous media substrate in the present embodiment uses filter paper, in the present embodiment
It also may include the step of preparing runner on filter paper either directlying adopt the filter paper for being prepared with runner.This implementation
A kind of embodiment of example building runner is that curable liquid hydrophobic material is applied on porous media substrate, is dredged to liquid
Water material carries out curing process after permeating porous media substrate completely, can form hydrophobic runner after liquid hydrophobic material solidification
Wall, flow path wall is around runner is formed, and in addition to this, the present invention can also construct runner, the present invention couple using other known methods
This is without limitation.
Step 2
Apply wax liquor in the valve region of runner, infiltrates through wax liquor in the filter paper at the valve region on runner.
Specifically, the present embodiment first uses wax printer that the wax of molten condition is printed upon valve region, then valve region is carried out
Heating, so that the wax that valve region has been cured melts again can be obtained above-mentioned wax liquor.In addition it is also possible to which solid state wax is molten
Solution forms solution in volatilizable solvent, and solution is then coated in valve region, can be so that solid state wax after solvent volatilization
It is attached on filter paper, finally valve region is heated, so that above-mentioned wax can be obtained in the solid state wax thawing of valve region
Liquid.
Step 3
Cooling and solidifying is carried out after above-mentioned wax liquor permeates filter paper completely can form wax valve in runner, and wax valve will flow
Road is divided into mutual disconnected at least two region, and mutual disconnected conversion zone and detection zone are specifically divided into the present embodiment
Domain.By increasing more wax valves, runner can be divided into more disconnected regions.
Step 4
Plastic packaging is carried out to the one side of filter paper, to prevent the leakage of reagent solution.
Referring to Fig. 2, show that the present invention is based on the front views of micro-fluidic chip one embodiment made from the above method.Such as
Shown in figure, micro-fluidic chip includes porous media substrate 1, flow path wall 2 and valve region 3, and flow path wall 2 surrounds and forms runner, valve
Runner is divided into mutually disconnected conversion zone 101 and detection zone 102 by door region 3.
The invention also discloses a kind of liquid flow control methods based on above-mentioned micro-fluidic chip, comprising the following steps:
Reagent is added in conversion zone 101, after the reagent of conversion zone 101 has completely reacted or is thoroughly mixed, removes valve
The wax in door region 3, reagent can be detected from 101 flow detection region 102 of conversion zone.
The method that valve is removed in the present embodiment is to apply the solvent that can dissolve wax to valve, and solvent can be acetone, isopropyl
Alcohol, toluene, acetonitrile or n-hexane.Referring to Fig. 3, different solvents are shown to the solute effect of valve.As shown, opening same
The usage amount of the valve of sample size, toluene and acetonitrile is minimum.
Referring to Fig. 4, the time diagram of different solvents dissolution valve is shown.Under the premise of same dose, toluene tool
There is shortest solution rate, therefore consider to shorten the dissolution time of valve region wax, it is preferred to use toluene is as removal valve
Solvent.
Referring to Fig. 5, influence schematic diagram of the valve transparency of the present invention to valve barriering effect is shown.Before valve preparation
It needs first to draw out design drawing, then valve is printed on porous media substrate according to the pattern on design drawing, wherein designing
Each region has different transparencies on figure, and the wax of the higher region printing of transparency is fewer, i.e. the transparency of design drawing (can also
Referred to as valve transparency) it is inversely proportional with the wax amount printed, as shown, the wax amount of printing is fewer, then the wax of valve region is fewer,
The obstructing capacity of valve is weaker, but solvent volume needed for dissolving valve is fewer；Conversely, the wax amount of printing is more, valve area
Domain wax is more, and the obstructing capacity of valve is stronger, and solvent volume needed for dissolving valve is more.On the other hand, the barrier energy of valve
Power is also by the effect length of valve, and valve length is longer, and obstructing capacity is stronger, i.e. the longer valve of length, allows less wax
Amount, however the wax measurer has lower limit, i.e., after wax amount is less than setting value, even if increasing valve length cannot guarantee that with enough
Obstructing capacity.
Usage mode in conjunction with Fig. 2, in the present embodiment are as follows: starch is added dropwise in the detection zone of micro-fluidic chip 102 first
Indicator, dropwise reaction object p-methyl benzenesulfonic acid and potassium iodide in nonoverlapping two zonules of 101 internal mutual of conversion zone, really
Two solvents are protected not contact.It is separated between conversion zone 101 and detection zone 102 by valve region 3.When detection,
Conversion zone 101 instills Potassiumiodate sample, and Potassiumiodate sample generates triiodo yin after sufficiently reacting with the reactant of conversion zone 101
Then ion removes the wax of valve region 3, the sample with triiodo anion flows into detection zone 102, anti-with starch indicator
It answers and develops the color.If not set valve, Potassiumiodate sample will flow into detection zone 102 before complete reaction, cause to generate
Triiodo anion amount substantially reduce, thus significantly reduced with the react length of colour developing of starch indicator, influence detection effect
Fruit.
It is to be illustrated to what preferable implementation of the invention carried out, but the invention is not limited to the implementation above
Example, those skilled in the art can also make various equivalent variations on the premise of without prejudice to spirit of the invention or replace
It changes, these equivalent deformations or replacement are all included in the scope defined by the claims of the present application.
Claims (9)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811330430.4A CN109603929B (en) | 2018-11-09 | 2018-11-09 | Manufacturing method of valve on chip, micro-fluidic chip and liquid flow control method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811330430.4A CN109603929B (en) | 2018-11-09 | 2018-11-09 | Manufacturing method of valve on chip, micro-fluidic chip and liquid flow control method |
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| CN109603929A true CN109603929A (en) | 2019-04-12 |
| CN109603929B CN109603929B (en) | 2021-01-05 |
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Cited By (4)
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| CN112067383A (en) * | 2020-08-13 | 2020-12-11 | 哈尔滨工业大学（深圳） | Method and chip for controlling liquid flow in porous medium material |
| CN113198552A (en) * | 2021-03-29 | 2021-08-03 | 苏州深得源健康科技有限公司 | Micro-fluidic chip for extracting nucleic acid and preparation method and application method thereof |
| CN114235793A (en) * | 2021-12-09 | 2022-03-25 | 汪开继 | Noninvasive uric acid instant detection device, detection system and detection method |
| CN117000323A (en) * | 2023-09-22 | 2023-11-07 | 北京芯迈微生物技术有限公司 | Microfluidic chip |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112067383A (en) * | 2020-08-13 | 2020-12-11 | 哈尔滨工业大学（深圳） | Method and chip for controlling liquid flow in porous medium material |
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| CN114235793A (en) * | 2021-12-09 | 2022-03-25 | 汪开继 | Noninvasive uric acid instant detection device, detection system and detection method |
| CN117000323A (en) * | 2023-09-22 | 2023-11-07 | 北京芯迈微生物技术有限公司 | Microfluidic chip |
| CN117000323B (en) * | 2023-09-22 | 2024-01-12 | 北京芯迈微生物技术有限公司 | A microfluidic chip |
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| CN109603929B (en) | 2021-01-05 |
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