CN109603562A - A kind of enzyme preparation and its application method for beer filtration Membrane cleaning - Google Patents
A kind of enzyme preparation and its application method for beer filtration Membrane cleaning Download PDFInfo
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- CN109603562A CN109603562A CN201811565752.7A CN201811565752A CN109603562A CN 109603562 A CN109603562 A CN 109603562A CN 201811565752 A CN201811565752 A CN 201811565752A CN 109603562 A CN109603562 A CN 109603562A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2321/00—Details relating to membrane cleaning, regeneration, sterilization or to the prevention of fouling
- B01D2321/16—Use of chemical agents
- B01D2321/166—Use of enzymatic agents
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Abstract
The present invention provides a kind of enzyme preparation for beer filtration Membrane cleaning, by mass percentage, including acid protease 0.5%-1%, neutral proteinase 0.5%-1%, dextranase 5%-20%, neutral cellulase 5%-10%, acidic cellulase 10%-30%, pectase 10%-30%, the sodium chloride solution 8%-69% that mass concentration is 10%.The enzyme preparation passes through the synergistic function between all kinds of single enzyme preparations, can the substances such as effectively hydrolyzing yeast, albumen, glucan, colloidal type, membrane flux can be significantly improved, sodium hydroxide wash number is reduced, extend filtering membrane lifetime and reduce production cost.The present invention also provides a kind of application methods of enzyme preparation for beer filtration Membrane cleaning, it is 0.05%-0.5% that the enzyme preparation, which is diluted to mass concentration with warm water, then to washed-alkali cleaning-washing after the filter membrane that is in neutrality cleaned using the enzyme preparation, can the substances such as fast hydrolyzing yeast, albumen, glucan, colloidal type, it is simple to operate.
Description
Technical field
The present invention relates to filtering technical field of membrane more particularly to a kind of enzyme preparation for beer filtration Membrane cleaning and
Its application method.
Background technique
It, can at normal temperature can be the residual in draft beer using supermicro filtration membrane filtering technique in draft beer production
Yeast and pollution sources are separated off, therefore can replace high-temperature instantaneous sterilization or pasteurization, to keep draft beer original
Flavor, and when energy-saving, therefore supermicro filtration membrane technology is widely used.And the quality of film properties is filtered to product
Quality has vital effect.Low-temperature filtering is the key link for producing draft beer, but filtered in actual production
Cheng Zhong, filter membrane are easy to be blocked by yeast, albumen, glucan, colloid substance and certain inorganic salts etc., or even on filter membrane
Agglomeration causes about 4000 tons of draft beers of every filtering just to need replacing new filter membrane, spends ten thousand yuan of about 30-40, makes to be produced into
This pressure is excessive.
Currently used cleaning method is physics and chemical method, and physical method is clear to the carry out such as surface lumps, impurity
Reason, or directly with the hot water of certain pressure or backpressure flushing etc.；Chemical method is using acid, alkali and surfactant, metal
Chelating agent etc. dissolves the blocking substance of filter membrane, emulsion dispersion reaches cleaning effect, is finally rinsed again with a large amount of water.
Currently used cleaning sequence is: warm water, thermokalite, hot water, cold water are successively cleaned and are regenerated.Water consumption is big, is easy to filter membrane
It causes to damage, and pollutes environment, it is undesirable to the organic species removal effect of filter membrane blocking.
Summary of the invention
The purpose of the present invention is to provide a kind of enzyme preparation for beer filtration Membrane cleaning, which passes through each
Synergistic function between class enzyme preparation, the organic and inorganic matter that can be attached on the filter membrane of effectively hydrolyzing draft beer are residual
Slag.
The object of the invention is also to provide a kind of application methods of enzyme preparation for beer filtration Membrane cleaning, lead to
The application method for crossing the enzyme preparation can quickly and efficiently hydrolyze the organic and inorganic matter attached on the filter membrane of draft beer
Residue, it is simple to operate.
In order to achieve the above object, present invention firstly provides a kind of enzyme preparations for beer filtration Membrane cleaning, by matter
Measure percentage meter, including following components: acid protease 0.5%-1%, neutral proteinase 0.5%-1%, dextranase 5%-
20%, neutral cellulase 5%-10%, acidic cellulase 10%-30%, pectase 10%-30% and mass concentration are
10% sodium chloride solution 8%-69%.
As a further improvement of the above technical scheme, by mass percentage, the dosage of dextranase is 8%-15%,
The dosage of acidic cellulase is 15%-25%, and the dosage of pectase is 15%-25%, the sodium chloride that mass concentration is 10%
The dosage of solution is 25%-55%.
As a further improvement of the above technical scheme, by mass percentage, including following components: acid protease
0.8%, neutral proteinase 0.8%, dextranase 15%, neutral cellulase 8%, acidic cellulase 20%, pectase
15%, the sodium chloride solution 40.4% that mass concentration is 10%.
A kind of application method of the enzyme preparation for beer filtration Membrane cleaning, comprising the following steps:
Step a. takes 0.5-1 parts of acid protease respectively, and 0.5-1 parts of neutral proteinase, 5-20 parts of dextranase, neutrality is fine
Tie up plain 5-10 part of enzyme, 10-30 part of acidic cellulase, 10-30 parts of pectase and mass concentration for 10% sodium chloride solution 8-
69 parts are mixed to form enzyme preparation, and the enzyme preparation is diluted with water to the enzymes soln that mass concentration is 0.05%-0.5%；
Step b. successively washed beer filtration film to be cleaned, alkali cleaning, sterile washing obtain mistake to be processed
Filter membrane；
Step c. is recycled in rinse bath flows into the enzymes soln, and the filter membrane to be processed is put into the cleaning
It is cleaned in slot, the enzymes soln is discharged after the completion of cleaning.
As a further improvement of the above technical scheme, after the completion of the step c, continue to adopt the filter membrane to be processed
Use sterile water wash.
As a further improvement of the above technical scheme, in the step a, the temperature of the water is 45 DEG C -55 DEG C.
As a further improvement of the above technical scheme, in the step a, the temperature of the water is 50 DEG C.
As a further improvement of the above technical scheme, in the step c, the scavenging period of the filter membrane to be processed is
1-6h。
As a further improvement of the above technical scheme, in the step c, the rate of circulating flow of the enzymes soln is
20-30kL/h。
As a further improvement of the above technical scheme, in the step b, the sterile water wash to sterile water wash liquid
PH be 6.5-7.5.
Beneficial effects of the present invention: the present invention provides a kind of enzyme preparation for beer filtration Membrane cleaning, including acid
Property proteinase-10 .5%-1%, neutral proteinase 0.5%-1%, dextranase 5%-20%, neutral cellulase 5%-10%,
Acidic cellulase 10%-30%, pectase 10%-30%, the sodium chloride solution 8%-69% that mass concentration is 10%, the enzyme
Preparation, being capable of effectively hydrolyzing yeast, albumen, glucan, colloidal type by the synergistic function between all kinds of single enzyme preparations
Equal substances can significantly improve membrane flux, reduce sodium hydroxide wash number, extend filtering membrane lifetime and reduce production cost.
The present invention also provides a kind of application methods of enzyme preparation for beer filtration Membrane cleaning, which is used
Warm water is diluted to the enzymes soln that mass concentration is 0.05%-0.5%, then to washed-alkali cleaning-washing after be in
Property filter membrane carry out enzymes soln wash cycles, can the substances such as fast hydrolyzing yeast, albumen, glucan, colloidal type, operation
It is simple and convenient.
At the same time, which can be used in combination with any cleaning agent, only need to be clear with pure water after other modes cleaning
PH is washed till to reuse for neutral left and right.
To enable the above objects, features and advantages of the present invention to be clearer and more comprehensible, preferred embodiment is cited below particularly, makees detailed
It is described as follows.
Specific embodiment
Term as used herein:
" by ... preparation " it is synonymous with "comprising".Term "comprising" used herein, " comprising ", " having ", " containing "
Or its any other deformation, it is intended that cover non-exclusionism includes.For example, composition, step, method comprising listed elements,
Product or device are not necessarily limited to those elements, but may include not expressly listed other elements or such composition, step
Suddenly, method, product or the intrinsic element of device.
Conjunction " by ... form " exclude any element that do not point out, step or component.If in claim,
This phrase will make claim closed, so that it is not included the material in addition to the material of those descriptions, but relative
Except customary impurities.When phrase " by ... form " be rather than immediately following theme in the clause that appears in claim main body after
When, only it is limited to element described in the clause；Other elements be not excluded the claim as a whole it
Outside.
Equivalent, concentration or other values or parameter are excellent with range, preferred scope or a series of upper limit preferred values and lower limit
When the Range Representation that choosing value limits, this should be understood as specifically disclosing by any range limit or preferred value and any range
Any pairing of lower limit or preferred value is formed by all ranges, regardless of whether the range separately discloses.For example, when open
When range " 1~5 ", described range should be interpreted as including range " 1~4 ", " 1~3 ", " 1~2 ", " 1~2 and 4~
5 ", " 1~3 and 5 " etc..When numberical range is described herein, unless otherwise stated, otherwise the range is intended to include its end
Value and all integers and score in the range.
" mass parts " refer to the basic measurement unit for indicating the mass ratio relationship of multiple components, and 1 part can indicate arbitrary list
Position quality, can such as be expressed as 1g, may also indicate that 2.689g etc..If we say that the mass parts of component A are a parts, the matter of B component
Measuring part is b parts, then it represents that the quality of component A and the mass ratio a:b of B component.Alternatively, indicating that the quality of component A is aK, B group
The quality divided is bK (K is arbitrary number, indicates multiplying factor).It can not misread, unlike mass fraction, all components
The sum of mass parts be not limited to 100 parts of limitation.
"and/or" is used to indicate that one of illustrated situation or both may to occur, for example, A and/or B includes (A
And B) and (A or B)；
A kind of enzyme preparation for beer filtration Membrane cleaning, by mass percentage, including following components: acid egg
White enzyme 0.5%-1%, neutral proteinase 0.5%-1%, dextranase 5%-20%, neutral cellulase 5%-10% are acid
Cellulase 10%-30%, pectase 10%-30%, the sodium chloride solution 8%-69% that mass concentration is 10%.
In this embodiment, this enzyme preparation is liquid dosage form enzyme preparation.It is easy to use using liquid dosage form, and
The energy is saved when production, purity is high, clear prevent secondary pollution.
Certainly, in other embodiments, the enzyme preparation can also be powder, particle, coating or other immobilization shapes
Formula.
In this embodiment, inventor passes through the life first screened and have degradation function to the residue of filter membrane agglomeration
Object enzyme class is obtained using various biological enzyme viabilities and the synergistic function between them by a large amount of combination experiment
To above-mentioned enzyme preparation component and content, can residue on high efficiency filter film, such as hydrolyzed yeast, albumen, glucan, colloidal type
Substance.
The enzyme preparation of present embodiment is suitable for daily beer filtration film situ cleaning (CIP) process or works as
When filtering film device pressure is more than 300mbar.It can be selected for daily beer filtration film situ cleaning (CIP) process lower
The enzymes soln of concentration, for higher concentration to can be selected when filter membrane equipment pressure is more than the filtering Membrane cleaning of 300mbar
Enzymes soln.
Acid protease, such as can be account for enzyme preparation 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% etc..
Neutral proteinase, such as can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% etc. of the total enzyme preparation of Zhan.
Dextranase, for example, can for the total enzyme preparation of Zhan 5%, 7%, 9%, 10%, 12%, 13%, 15%, 16%,
17%, 18%, 19%, 20% etc..
Neutral cellulase, such as can be 5%, 6%, 7%, 8%, 9%, the 10% of the total enzyme preparation of Zhan.
Acidic cellulase, for example, can for the total enzyme preparation of Zhan 10%, 11%, 14%, 15%, 18%, 20%,
22%, 25%, 27%, 30% etc..
Pectase, for example, can for the total enzyme preparation of Zhan 10%, 12%, 14%, 15%, 17%, 19%, 21%, 22%,
24%, 25%, 27%, 29%, 30% etc..
Sodium chloride solution, for example, can for the total enzyme preparation of Zhan 8%, 10%, 15%, 20%, 25%, 30%, 35%,
40%, 45%, 50%, 55%, 60%, 65%, 69% etc..
The enzyme preparation includes following components as a preferred method: acid protease 0.5%-1%, neutral proteinase
0.5%-1%, dextranase 8%-15%, neutral cellulase 5%-10%, acidic cellulase 15%-25%, pectase
15%-25%, the sodium chloride solution 25%-55% that mass concentration is 10%.
As a kind of more preferable mode, which includes following components: acid protease 0.8%, neutral proteinase
0.8%, dextranase 15%, neutral cellulase 8%, acidic cellulase 20%, pectase 15%, mass concentration 10%
Sodium chloride solution 40.4%.
A kind of application method of the enzyme preparation for beer filtration Membrane cleaning, comprising the following steps:
Step a. takes 0.5-1 parts of acid protease respectively, and 0.5-1 parts of neutral proteinase, 5-20 parts of dextranase, neutrality is fine
Plain 5-10 parts of the enzyme of dimension, 10-30 parts of acidic cellulase, 10-30 parts of pectase, the sodium chloride solution 8- that mass concentration is 10%
69% part is mixed to form enzyme preparation, and it is molten that the enzyme preparation is diluted with water to the enzyme preparation that mass concentration is 0.05%-0.5%
Liquid；
Step b. successively washed beer filtration film to be cleaned, alkali cleaning, sterile washing obtain mistake to be processed
Filter membrane；
Step c. is recycled in rinse bath flows into the enzymes soln, and the filter membrane to be processed is put into the cleaning
It is cleaned in slot, the enzymes soln is discharged after the completion of cleaning.
Acid protease, such as can be 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part etc..
Neutral proteinase, such as can be 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part etc..
Dextranase, such as can be 5 parts, 6 parts, 7 parts, 8 parts, 10 parts, 12 parts, 13 parts, 15 parts, 17 parts, 20 parts etc..
Neutral cellulase, such as can be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts etc..
Acidic cellulase, such as can be 10 parts, 11 parts, 14 parts, 15 parts, 18 parts, 20 parts, 22 of the total enzyme preparation of Zhan
Part, 25 parts, 27 parts, 30 parts etc..
Pectase, for example, can for 10 parts of the total enzyme preparation of Zhan, 12 parts, 14 parts, 15 parts, 17 parts, 19 parts, 21 parts, 22 parts,
24 parts, 25 parts, 27 parts, 29 parts, 30 parts etc..
Sodium chloride solution, such as can be 8 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 of the total enzyme preparation of Zhan
Part, 45 parts, 50 parts, 55 parts, 60 parts, 65 parts, 69 parts etc..
In this specific embodiment, the sequence of the step a and step b and regardless of front and back can carry out, can step simultaneously
A prior to step b, can also step b prior to step a.
Optionally, after the completion of the step c, sterile water can be used to the filter membrane to be processed according to cleaning situation continued
Cleaning.
Optionally, in the step a, the temperature of the water is 45 DEG C -55 DEG C.
Optionally, in the step a, the temperature of the water is 50 DEG C.
Enzyme has enzymatic activity most strong, the feature of enzymatic reaction speed maximum in optimum temperature range.In suitable temperature
In range, temperature is every to increase 10 DEG C, and enzymatic reaction speed can correspondingly increase 1~2 times.The optimum temperature of enzyme in different organisms
It is different.But the excessively high or too low enzyme that will lead to inactivates.In the present embodiment, enzyme preparation has efficient when water temperature is 50 DEG C
Catalysis and hydrolysis ability.
Optionally, in the step b, the pH of the sterile water wash to sterile water wash liquid is 6.5-7.5.
Preferably, in the step b, the pH of the sterile water wash to sterile water wash liquid is 7.
Optionally, in the step c, the scavenging period of the filter membrane to be processed is 1-6h.
Optionally, in the step c, the rate of circulating flow of the enzymes soln is 20-30kL/h.
Embodiment 1
The present embodiment 1 provides a kind of enzyme preparation for beer filtration Membrane cleaning, according to the mass fraction, including it is following
Component: 0.5 part of acid protease, 0.5 part of neutral proteinase, 5 parts of dextranase, 5 parts of neutral cellulase, acidic cellulase
10 parts, 10 parts of pectase, mass concentration is 69 parts of sodium chloride solution of 10%.
Embodiment 2
The present embodiment 2 provides a kind of enzyme preparation for beer filtration Membrane cleaning, by mass percentage, including with
Lower component: 1 part of acid protease, 1 part of neutral proteinase, 20 parts of dextranase, 10 parts of neutral cellulase, acidic cellulase
30 parts, 30 parts of pectase, mass concentration is 8 parts of sodium chloride solution of 10%.
Embodiment 3
The present embodiment 3 provides a kind of enzyme preparation for beer filtration Membrane cleaning, by mass percentage, including with
Lower component: 0.8 part of acid protease, 0.8 part of neutral proteinase, 15 parts of dextranase, 8 parts of neutral cellulase, acid fiber
20 parts of plain enzyme, 20 parts of pectase, mass concentration is 35.4 parts of sodium chloride solution of 10%.
Embodiment 4
The present embodiment 4 provides a kind of enzyme preparation for beer filtration Membrane cleaning, by mass percentage, including with
Lower component: 0.8 part of acid protease, 0.8 part of neutral proteinase, 15 parts of dextranase, 8 parts of neutral cellulase, acid fiber
20 parts of plain enzyme, 15 parts of pectase, mass concentration is 40.4 parts of sodium chloride solution of 10%.
Comparative example 1
This comparative example 1 provides a kind of enzyme preparation for beer filtration Membrane cleaning, and enzyme preparation type, which is removed, lacks acid
Except property protease, remaining is identical as the type of embodiment 4, content by mass percentage, including following components: neutral egg
0.8 part of white enzyme, 15 parts of dextranase, 8 parts of neutral cellulase, 20 parts of acidic cellulase, 15 parts of pectase, mass concentration is
41.2 parts of 10% sodium chloride solution.
Comparative example 2
This comparative example 2 provides a kind of enzyme preparation for beer filtration Membrane cleaning, and enzyme preparation type, which is removed, lacks Portugal
Except dextranase, remaining is identical as the type of embodiment 4, content by mass percentage, including following components: acidic protein
0.8 part of enzyme, 0.8 part of neutral proteinase, 8 parts of neutral cellulase, 20 parts of acidic cellulase, 15 parts of pectase, mass concentration
For 55.4 parts of sodium chloride solution of 10%.
Comparative example 3
This comparative example 3 provides a kind of enzyme preparation for beer filtration Membrane cleaning, enzyme preparation type except lacking in
Property cellulase except, remaining is identical as the type of embodiment 4, content by mass percentage, including following components: acid
.8 parts of proteinase-10,0.8 part of neutral proteinase, 15 parts of dextranase, 20 parts of acidic cellulase, 15 parts of pectase, mass concentration
For 48.4 parts of sodium chloride solution of 10%.
Comparative example 4
This comparative example 4 provides a kind of enzyme preparation for beer filtration Membrane cleaning, selects 100 parts of dextranase.
Comparative example 5
This comparative example 4 provides a kind of enzyme preparation for beer filtration Membrane cleaning, selects acidic cellulase 100
Part.
Comparative example 6
This comparative example 4 provides a kind of enzyme preparation for beer filtration Membrane cleaning, selects 100 parts of pectase enzyme.
Comparative example 7
To the filter membrane blocked by residue, filtering film device pressure carries out CIP cleaning when being more than 300mbar, and CIP is clear
Washing step includes washing-alkali cleaning-washing-pickling-washing, and circulation is three times.
The raw material of one, above-described embodiment 1 to 4 of table and comparative example 1 to 7 composition
Cleaning test control
Above-described embodiment 1 to 4 and comparative example 1 to 6 are cleaned, comprising the following steps:
Content of each raw material as described in table one is taken respectively, is mixed to form enzyme preparation, is 50 DEG C by the enzyme preparation temperature
Water be diluted to mass concentration be 0.5% enzymes soln；
Beer filtration film to be cleaned is washed, alkali cleaning, the sterile pH for being washed to sterile water wash liquid be 7,
Obtain filter membrane to be processed；
Circulation flows into the enzymes soln in rinse bath, by the filter membrane to be processed be put into the rinse bath into
Row cleaning, the time of cleaning are 2h, and the rate of circulating flow of enzymes soln is 20-30kL/h, and the enzyme system is discharged after the completion of cleaning
Agent solution.
Analysis of experimental results:
After the raw material proportioning for taking above-described embodiment 1 to 4 and comparative example 1 to 6, to by residue blocking, its filter membrane
Filter membrane when equipment pressure is more than 300mbar carries out the cleaning method as shown in cleaning test control；It is carried out simultaneously as compared
Mode described in example 7 cleans filter membrane, respectively carries out membrane flux and film recovery rate statistics, logical with the film of normal filtration film
Amount is for 650hL/ (10 inch):
2 routine CIP of table cleaning and the enzyme flux Contrast on effect washed
The enzyme preparation cleaning filter membrane of embodiment 1-4 can significantly improve membrane flux, while film it can be seen from upper table
Recovery rate is high, and the cleaning effect of embodiment 4 is especially excellent.The cleaning effect of embodiment 1-4 is significantly larger than the routine of comparative example 7
CIP cleaning method.At the same time, filter membrane is cleaned by comparing the enzyme preparation of example 1-4, although cleaning effect and conventional CIP
Cleaning effect is preferable, but is not much different, and effect is much worse than embodiment 1-4.Effect is best for embodiment in embodiment 1-4
4, it is secondly embodiment 3, Examples 1 and 2 are close, although embodiment 1-4 effect is not much different, bring economic benefit phase
Poor obvious, the economic benefit of embodiment 4 will be significantly larger than embodiment 1-3.Therefore in the technical solution of the application, acidic protein
Enzyme, neutral proteinase, dextranase, neutral cellulase, acidic cellulase, pectase, which are not only simple combination, to be made
With, the enzyme preparation by the synergistic function between all kinds of single enzyme preparations can effectively hydrolyzing yeast, albumen, Portugal it is poly-
The substances such as sugar, colloidal type can significantly improve membrane flux.
The numberical range of each technological parameter as involved in the present invention can not all embody in the above-described embodiments,
As long as but those skilled in the art's envisioned any numerical value fallen into the above-mentioned numberical range completely can be implemented this
Invention also includes any combination of occurrence in several numberical ranges certainly.Herein, due to space considerations, be omitted to
Out in certain one or more numberical range occurrence embodiment, this disclosure for being not to be construed as technical solution of the present invention do not fill
Point.
The Applicant declares that the present invention is explained by the above embodiments detailed process equipment and process flow of the invention,
But the present invention is not limited to the above detailed process equipment and process flow, that is, it is above-mentioned detailed not mean that the present invention must rely on
Process equipment and process flow could be implemented.It should be clear to those skilled in the art, any improvement in the present invention,
Addition, concrete mode selection of equivalence replacement and auxiliary element to each raw material of product of the present invention etc., fall in protection of the invention
In range.
Claims (10)
1. a kind of enzyme preparation for beer filtration Membrane cleaning, which is characterized in that by mass percentage, including with the following group
Point: acid protease 0.5%-1%, neutral proteinase 0.5%-1%, dextranase 5%-20%, neutral cellulase 5%-
The sodium chloride solution 8%- that 10%, acidic cellulase 10%-30%, pectase 10%-30% and mass concentration are 10%
69%.
2. enzyme preparation according to claim 1, which is characterized in that by mass percentage, the dosage of dextranase is
8%-15%, the dosage of acidic cellulase are 15%-25%, and the dosage of pectase is 15%-25%, mass concentration 10%
Sodium chloride solution dosage be 25%-55%.
3. enzyme preparation according to claim 1, which is characterized in that by mass percentage, including following components: acid egg
White enzyme 0.8%, neutral proteinase 0.8%, dextranase 15%, neutral cellulase 8%, acidic cellulase 20%, pectin
Enzyme 15%, the sodium chloride solution 40.4% that mass concentration is 10%.
4. a kind of application method of the enzyme preparation for beer filtration Membrane cleaning, which comprises the following steps:
Step a. takes 0.5-1 parts of acid protease respectively, and 0.5-1 parts of neutral proteinase, 5-20 parts of dextranase, neutral fibre is plain
5-10 parts of enzyme, 10-30 parts of acidic cellulase, 10-30 parts of pectase and mass concentration are 8-69 parts of sodium chloride solution of 10%
It is mixed to form enzyme preparation, the enzyme preparation is diluted with water to the enzymes soln that mass concentration is 0.05%-0.5%；
Step b. successively washed beer filtration film to be cleaned, alkali cleaning, sterile washing obtain filter membrane to be processed；
Step c. is recycled in rinse bath flows into the enzymes soln, and the filter membrane to be processed is put into the rinse bath
It is cleaned, the enzymes soln is discharged after the completion of cleaning.
5. the application method of enzyme preparation according to claim 4, which is characterized in that after the completion of the step c, continue to institute
Filter membrane to be processed is stated using sterile water wash.
6. the application method of enzyme preparation according to claim 4, which is characterized in that in the step a, the temperature of the water
It is 45 DEG C -55 DEG C.
7. the application method of enzyme preparation according to claim 4, which is characterized in that in the step a, the temperature of the water
It is 50 DEG C.
8. the application method of enzyme preparation according to claim 4, which is characterized in that in the step b, the sterile water is clear
The pH for being washed till sterile water wash liquid is 6.5-7.5.
9. the application method of enzyme preparation according to claim 4, which is characterized in that in the step c, the mistake to be processed
The scavenging period of filter membrane is 1-6h.
10. the application method of enzyme preparation according to claim 4, which is characterized in that in the step c, the enzyme preparation
The rate of circulating flow of solution is 20-30kL/h.
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Cited By (1)
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| US12371641B2 (en) | 2021-08-02 | 2025-07-29 | Ecolab Usa Inc. | Booster composition for cleaning fermentation equipment and methods of use |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01304007A (en) * | 1988-05-31 | 1989-12-07 | Yuho Chem Kk | Washing agent for ion exchange membrane |
| WO1996023579A1 (en) * | 1995-02-01 | 1996-08-08 | Henkel-Ecolab Gmbh & Co. Ohg | Method of cleaning membrane filters |
| CN1295610A (en) * | 1998-03-27 | 2001-05-16 | 诺沃挪第克公司 | Acid cleaning composition containing acid protease |
| CN1803034A (en) * | 2005-01-10 | 2006-07-19 | 中国农业大学 | Method for recovering filtration rate of hyperfiltration membrane |
| CN1970743A (en) * | 2006-11-28 | 2007-05-30 | 天津达美科技有限公司 | Ultrafiltration cleaning of enzyme |
| CN102078770A (en) * | 2010-12-02 | 2011-06-01 | 华南农业大学 | Solid compound enzyme for cleaning filtering membrane of draught beer |
| CN102133504A (en) * | 2010-01-25 | 2011-07-27 | 滕国新 | Filter membrane cleaning agent and cleaning technology |
| CN102492663A (en) * | 2011-12-01 | 2012-06-13 | 广西大学 | Pure draft beer film cleaning and regenerating enzyme preparation and cleaning method using same |
-
2018
- 2018-12-20 CN CN201811565752.7A patent/CN109603562A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01304007A (en) * | 1988-05-31 | 1989-12-07 | Yuho Chem Kk | Washing agent for ion exchange membrane |
| WO1996023579A1 (en) * | 1995-02-01 | 1996-08-08 | Henkel-Ecolab Gmbh & Co. Ohg | Method of cleaning membrane filters |
| CN1295610A (en) * | 1998-03-27 | 2001-05-16 | 诺沃挪第克公司 | Acid cleaning composition containing acid protease |
| CN1803034A (en) * | 2005-01-10 | 2006-07-19 | 中国农业大学 | Method for recovering filtration rate of hyperfiltration membrane |
| CN1970743A (en) * | 2006-11-28 | 2007-05-30 | 天津达美科技有限公司 | Ultrafiltration cleaning of enzyme |
| CN102133504A (en) * | 2010-01-25 | 2011-07-27 | 滕国新 | Filter membrane cleaning agent and cleaning technology |
| CN102078770A (en) * | 2010-12-02 | 2011-06-01 | 华南农业大学 | Solid compound enzyme for cleaning filtering membrane of draught beer |
| CN102492663A (en) * | 2011-12-01 | 2012-06-13 | 广西大学 | Pure draft beer film cleaning and regenerating enzyme preparation and cleaning method using same |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12371641B2 (en) | 2021-08-02 | 2025-07-29 | Ecolab Usa Inc. | Booster composition for cleaning fermentation equipment and methods of use |
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