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CN109477086A - 一种基因组dna特异性编辑方法和应用 - Google Patents

一种基因组dna特异性编辑方法和应用 Download PDF

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Publication number
CN109477086A
CN109477086A CN201780043459.1A CN201780043459A CN109477086A CN 109477086 A CN109477086 A CN 109477086A CN 201780043459 A CN201780043459 A CN 201780043459A CN 109477086 A CN109477086 A CN 109477086A
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genome dna
editing method
specific editing
genome
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陈奇涵
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Abstract

一种调节核酸的甲基化/去甲基化状态的方法，具体为：一种可以在细胞内由sgRNA序列引导的在基因组上定点去除一个或多个甲基化碱基的方法。

Description

PCT国内申请，说明书已公开。

Claims (15)

  1. PCT国内申请，权利要求书已公开。
CN201780043459.1A 2016-07-13 2017-06-14 一种基因组dna特异性编辑方法和应用 Pending CN109477086A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201610550293X 2016-07-13
CN201610550293 2016-07-13
PCT/CN2017/088281 WO2018010516A1 (zh) 2016-07-13 2017-06-14 一种基因组dna特异性编辑方法和应用

Publications (1)

Publication Number Publication Date
CN109477086A true CN109477086A (zh) 2019-03-15

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US (1) US20230151341A1 (zh)
CN (1) CN109477086A (zh)
WO (1) WO2018010516A1 (zh)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019041296A1 (zh) 2017-09-01 2019-03-07 上海科技大学 一种碱基编辑系统及方法
CN109021111B (zh) * 2018-02-23 2021-12-07 上海科技大学 一种基因碱基编辑器
CN111788232A (zh) * 2018-02-23 2020-10-16 上海科技大学 用于碱基编辑的融合蛋白
CN108753823B (zh) * 2018-06-20 2022-09-23 李广磊 利用碱基编辑技术实现基因敲除的方法及其应用
CN111165342A (zh) * 2020-01-19 2020-05-19 安徽省农业科学院水稻研究所 一种偏籼型水稻恢复系的选育方法
CN114540325B (zh) * 2022-01-17 2022-12-09 广州医科大学 靶向dna去甲基化的方法、融合蛋白及其应用
EP4479436A1 (en) * 2022-02-17 2024-12-25 Correctsequence Therapeutics Mutant cytidine deaminases with improved editing precision

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015089406A1 (en) * 2013-12-12 2015-06-18 President And Fellows Of Harvard College Cas variants for gene editing
WO2015133554A1 (ja) * 2014-03-05 2015-09-11 国立大学法人神戸大学 標的化したｄｎａ配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体
CN105112446A (zh) * 2015-06-25 2015-12-02 中国医学科学院基础医学研究所 使用单倍体干细胞高效建立遗传修饰动物模型的方法
WO2016025131A1 (en) * 2014-08-13 2016-02-18 E. I. Du Pont De Nemours And Company Genetic targeting in non-conventional yeast using an rna-guided endonuclease

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3744842A1 (en) * 2013-03-15 2020-12-02 The General Hospital Corporation Using truncated guide rnas (tru-grnas) to increase specificity for rna-guided genome editing

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015089406A1 (en) * 2013-12-12 2015-06-18 President And Fellows Of Harvard College Cas variants for gene editing
WO2015133554A1 (ja) * 2014-03-05 2015-09-11 国立大学法人神戸大学 標的化したｄｎａ配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体
WO2016025131A1 (en) * 2014-08-13 2016-02-18 E. I. Du Pont De Nemours And Company Genetic targeting in non-conventional yeast using an rna-guided endonuclease
CN105112446A (zh) * 2015-06-25 2015-12-02 中国医学科学院基础医学研究所 使用单倍体干细胞高效建立遗传修饰动物模型的方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BINYAMIN A. KNISBACHER等: "DNA Editing by APOBECs: A Genomic Preserver and Transformer", 《TRENDS IN GENETICS》 *
MARTA VAZQUEZ‑VILAR等: "A modular toolbox for gRNA–Cas9 genome engineering in plants based on the GoldenBraid standard", 《PLANT METHODS》 *
PRIYANGA WIJESINGHE等: "Efficient deamination of 5-methylcytosines in DNA by human APOBEC3A, but not by AID or APOBEC3G", 《NUCLEIC ACIDS RESEARCH》 *
阴晓彤等: "哺乳动物DNA甲基化修饰的动态调控机制", 《生命的化学》 *

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WO2018010516A1 (zh) 2018-01-18

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